Publications

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biological control of monilinia laxa and rhizopus stolonifer in postharvest of stone fruit by pantoea agglomerans eps125 and putative mechanisms of antagonism.treatment of stone fruits (apricot, peach and nectarine) with pantoea agglomerans strain eps125 decreased the incidence and diameter of lesions of brown rot caused by monilinia laxa and soft rot caused by rhizopus stolonifer. root control was achieved on fruits either wounded and subsequently inoculated with the pathogens or non-wounded and naturally infected from orchards. the efficacy of biocontrol was dependent on the concentration of the biocontrol agent and pathogen. at medium to low pathog ...200312781959
solid substrate production of epicoccum nigrum conidia for biological control of brown rot on stone fruits.production of conidia of epicoccum nigrum, a biocontrol agent of the fungal pathogen monilinia laxa, was tested in liquid- and solid-state fermentation. liquid fermentation was conducted in 250 ml erlenmeyer flasks containing 50 ml of a mineral medium (containing per litre: 20 g lactose, 10 g no3k, 1 g k2hpo4, 0.5 g mgso4.7h2o, and 1 ml of a minor-element solution), inoculated with 2 x 10(5) e. nigrum conidia ml(-1), and incubated at 20-25 degrees c and 150 rpm for 7 days. solid-state fermentati ...200415193802
characterization and pcr-based detection of benzimidazole-resistant isolates of monilinia laxa in california.monilinia laxa is a pathogen of brown rot of stone fruit and almond in california, causing blossom blights and fruit rots. in this study, low-level resistance to the benzimidazole fungicides benomyl and thiophanate-methyl was detected in field isolates of m laxa collected from stone fruits and almonds in california. low-resistant (lr) isolates grew in potato dextrose agar (pda) plates amended with benomyl and thiophanate-methyl at 1 and 5 microg ml(-1), respectively, but not in plates amended wi ...200515816017
lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi.this study evaluated the efficacy of lactic acid bacteria (lab) isolated from fresh fruits and vegetables as biocontrol agents against the phytopathogenic and spoilage bacteria and fungi, xanthomonas campestris, erwinia carotovora, penicillium expansum, monilinia laxa, and botrytis cinerea. the antagonistic activity of 496 lab strains was tested in vitro and all tested microorganisms except p. expansum were inhibited by at least one isolate. the 496 isolates were also analyzed for the inhibition ...200819204894
enhancing the adhesion of epicoccum nigrum conidia to peach surfaces and its relationship to the biocontrol of brown rot caused by monilinia laxa.to find a formulation of epicoccum nigrum conidia that enhances its adhesion to peach surfaces and improves its biocontrol efficacy against brown rot caused by monilinia laxa.201020163488
chemical composition and in vitro activity of plant extracts from ferula communis and dittrichia viscosa against postharvest fungi.f. communis and d. viscosa are perennial mediterranean weeds that have been used for different therapeutic purposes in traditional pharmacopeia. plant extracts were obtained from air dried d. viscosa young shoots (dva) and f. communis aerial part (fca) and roots (fcr) with n-hexane. the chemical compositions of the extracts were analyzed by hplc-dad, lc-ms (esi) and lc-q-tof techniques. two sesquiterpene lactones (inuviscolide, tomentosin) and three sesquiterpene acids (costic acid, hydroxycosti ...201121441864
cloning, characterization, expression and antifungal activity of an alkaline serine protease of aureobasidium pullulans pl5 involved in the biological control of postharvest pathogens.an alkaline protease gene was amplified from genomic dna and cdna of the antagonistic yeast-like fungus aureobasidium pullulans pl5, a biocontrol agent effective against monilinia laxa on stone fruit and botrytis cinerea and penicillium expansum on pome fruits. an open reading frame of 1248 bp encoding a 415-amino acid (aa) protein with a calculated molecular weight (m(r)) of 42.9 kda and an isoelectric point (pi) of 4.5 was characterized. the cdnaalp5 gene had an 18-amino acid signal peptide, o ...201122225984
characterization of three-dimensional spatial aggregation and association patterns of brown rot symptoms within intensively mapped sour cherry trees.background and aims characterization of spatial patterns of plant disease can provide insights into important epidemiological processes such as sources of inoculum, mechanisms of dissemination, and reproductive strategies of the pathogen population. whilst two-dimensional patterns of disease (among plants within fields) have been studied extensively, there is limited information on three-dimensional patterns within individual plant canopies. reported here are the detailed mapping of different sy ...201121335328
influence of additives on adhesion of penicillium frequentans conidia to peach fruit surfaces and relationship to the biocontrol of brown rot caused by monilinia laxa.additives, such as sucrose, d-sorbitol, glycerol, sodium alginate, carboxymethyl cellulose, silica gel, gelatine, non-fat skimmed milk and a commercial adhesive were added to conidia of penicillium frequentans at two different points in the production process of the formulation of this fungus to improve conidial adhesion. conidial adhesion was estimated as the number of p. frequentans conidia (no. conidia cm(-2)) and colony-forming units of p. frequentans per unit area (cfu cm(-2)) that adhered ...200818541321
capillary and gel electromigration techniques and maldi-tof ms--suitable tools for identification of filamentous fungi.microbial strains are now spreading out of their original geographical areas of incidence and previously adequate morphological identification methods often must be accompanied by a phenotypic characterization for the successful microbial identification. the fungal genus monilinia represents a suitable example. monilinia species represent important fruit pathogens responsible for major losses in fruit production. four closely related spp. of monilinia: monilinia laxa, monilinia fructigena, monil ...201122284891
high chlorogenic and neochlorogenic acid levels in immature peaches reduce monilinia laxa infection by interfering with fungal melanin biosynthesis.chlorogenic acid (cga) and its isomer, neochlorogenic acid (ncga), were found to be the major phenolic compounds in the flesh and peel of three peach cultivars. their concentrations are especially high in immature fruits (cga, 151-548 mg/kg; ncga, 85-380 mg/kg), whose resistance to the brown rot fungus, monilinia laxa , is very high. the concentrations of these two phenolic compounds decline in maturing fruits (cga, 77-181 mg/kg; ncga, 30-82 mg/kg), and this decline is associated with a concomit ...201121370882
effects of potassium sorbate on postharvest brown rot of stone fruit.the effect of potassium sorbate (k-sorb), a low-toxicity chemical, to control monilinia spp. was investigated. preliminary in vitro studies found the mic of k-sorb for conidial germination and mycelial growth was, respectively, 260 and 1,250 mg/ liter. immersion of naturally infected peach and nectarine fruit in a solution (15 g/liter) of k-sorb for 120 s reduced brown rot by over 80% in four of five trials. although treated fruits showed a significant reduction in firmness with respect to the c ...200818724757
effect of temperature and water activity on in vitro germination of monilinia spp.this study evaluated the effect of temperature (0-38 degrees c) and water activity (a(w): 0.87-0.99) on the lag phase prior to germination and the percentage of germination over time for monilinia laxa, monilinia fructicola and monilinia fructigena.201019519666
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