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staphylococcus nepalensis sp. nov., isolated from goats of the himalayan region.four coagulase-negative, novobiocin-resistant cocci, designated cw1(t), pm34, mm3 and rw78, were isolated from the respiratory tract of goats kept in the himalayan region. the four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after sds-page and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (eric)-pcr. strain cw1(t) showed highest 16s rdna sequence simila ...200314657137
effect of industrial and natural biocides on spoilage, pathogenic and technological strains grown in biofilm.this study aimed at investigating bactericide solutions effective on spoilage and pathogenic bacteria while preserving technological bacteria. two compounds of essential oil (thymol and eugenol), one essential oil of satureja thymbra and two industrial biocides (pe 270-30, brillo) were tested on technological strains (staphylococcus equorum, staphylococcus succinus and lactobacillus sakei) grown in monoculture biofilm and on a mixed biofilm of pathogenic bacteria (staphylococcus aureus, listeria ...200717188206
safety improvement and preservation of typical sensory qualities of traditional dry fermented sausages using autochthonous starter cultures.traditional dry fermented sausages are manufactured without addition of starter cultures in small-scale processing units, their fermentation relying on indigenous microflora. characterisation and control of these specific bacteria are essential for the sensory quality and the safety of the sausages. the aim of this study was to develop an autochthonous starter culture that improves safety while preserving the typical sensory characteristics of traditional sausages. an autochthonous starter compo ...200818573561
characterization of toxin production of coagulase-negative staphylococci isolated from food and starter cultures.in this study a comprehensive analysis of toxin production of food associated coagulase-negative staphylococci (cns) was investigated. the strains belong to the following staphylococcal species, staphylococcus carnosus, staphylococcus condimenti, staphylococcus equorum, staphylococcus piscifermentans, staphylococcus succinus, and staphylococcus xylosus, which were isolated from fermented food and starter cultures. a collection of 330 strains were analyzed with respect to their hemolytic activity ...200818752861
binding to extracellular matrix proteins and formation of biogenic amines by food-associated coagulase-negative staphylococci.in connection with a study on the dna microarray based detection of genes involved in safety and technologically relevant properties (seitter (née resch) et al., 2011), food-associated coagulase-negative staphylococci (cns) were investigated phenotypically with regard to their ability to bind to the extracellular matrix proteins (ecm) and to produce biogenic amines. the properties have been shown to be involved in the colonization of injured tissue and invasion into host cells as well as in phar ...201121333369
catalase-positive cocci in fermented sausage: variability due to different pork breeds, breeding systems and sausage production technology.the aim of this study was to compare the ecology of catalase-positive cocci (cpc) present in traditional fermented sausages produced using different breeds of pork, each of which was raised in two different environments and processed using two different technologies. semi-quantitative molecular methods were used to determine bacterial identities. almost all fermentations were characterised by a significant increase in cpc during the first few days of fermentation, reaching values of 10(5)-10(6)  ...201222202871
Combination of culture-dependent and culture-independent molecular methods for the determination of lactic microbiota in sucuk.In this study, the culture-dependent and culture-independent molecular methods were used for the identification of lactic acid bacteria (LAB) in sucuk a Turkish fermented dry sausage. On the one hand, the PCR-DGGE method targetting the V1 and V3 regions of 16S DNA was applied to DNA that was directly extracted from sucuk samples. On the other hand, rep-PCR fingerprinting was performed for the primary differentiation and grouping of the isolates, and the results were confirmed by sequencing of th ...201122209604
vancomycin-resistant gram-positive cocci isolated from the saliva of wild songbirds.we analyzed highly vancomycin-resistant gram-positive bacteria isolated from the saliva of migratory songbirds captured, sampled, and released from a bird-banding station in western kansas. individual bacterial isolates were identified by partial 16s rrna sequencing. most of the bacteria in this study were shown to be staphylococcus succinus with the majority being isolated from the american robin. some of these bacteria were shown to carry vana, vanb, and vanc vancomycin-resistance genes and ha ...201223224296
staphylococcal ecosystem of kitoza, a traditional malagasy meat product.kitoza is a traditional meat product from madagascar manufactured with strips of pork or beef. the process includes a first step of salting and mixing with spices followed by sun-drying or smoking step. as salting and drying select coagulase-negative staphylococci (cns), our aim was to identify the cns species in kitoza with the objective in the future of developing indigenous starters. microbial analyses revealed that the only pathogenic bacterium enumerated was staphylococcus aureus, which was ...201728187327
characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398.the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ...201626840519
screening for probiotic properties of strains isolated from feces of various human groups.the present study searched for potential probiotic strains from various human fecal samples. a total of 67 aerobic and 38 anaerobic strains were isolated from 5 different categories of human feces. systematic procedures were used to evaluate the probiotic properties of the isolated strains. these showed about 75-97% survivability in acidic and bile salt environments. adhesion to intestinal cell line caco-2 was also high. the isolates exhibited hydrophobic properties in hexadecane. the culture su ...201222923108
draft genome sequence of staphylococcus succinus subsp. succinus type strain dsm 14617, isolated from plant and soil inclusions within 25- to 35-million-year-old dominican amber.staphylococcus succinus subsp. succinus type strain dsm 14617 was isolated from plant and soil inclusions within 25- to 35-million-year-old dominican amber. the complete genome sequence of strain dsm 14617(t) includes a genome of 2.88 mb (32.94% g+c content) without any plasmids.201728126945
a novel approach to eliminate detection of contaminating staphylococcal species introduced during clinical testing.we describe here a strategy that can distinguish between staphylococcus species truly present in a clinical sample from contaminating staphylococcus species introduced during the testing process. contaminating staphylococcus species are present at low levels in pcr reagents and colonize lab personnel. to eliminate detection of contaminants, we describe an approach that utilizes addition of sufficient quantities of either non-target staphylococcal cells (staphylococcus succinus or staphylococcus ...201728225823
complete genome sequence of staphylococcus succinus 14bme20 isolated from a traditional korean fermented soybean food.the complete genome sequence of staphylococcus succinus 14bme20, isolated from a korean fermented soybean food and selected as a possible starter culture candidate, was determined. comparative genome analysis with s. succinus csm-77 from a triassic salt mine revealed the presence of strain-specific genes for lipid degradation in strain 14bme20.201728254985
the use of nucleosides and arginine as alternative energy sources by coagulase-negative staphylococci in view of meat fermentation.the ability of coagulase-negative staphylococci (cns) to use alternative energy sources in meat may partially explain their occurrence in fermented meats. of 61 cns strains tested, all metabolized adenosine and inosine in a meat simulation medium (msm). the ability to catabolize arginine via the arginine deiminase (adi) pathway varied between strains. all tested strains of staphylococcus carnosus and staphylococcus epidermidis possessed an arca gene and showed adi activity, whereas other species ...201424387852
staphylococcus equorum and staphylococcus succinus isolated from human clinical specimens.a polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as staphylococcus xylosus and three strains as ambiguous s. xylosus/staphylococcus equorum. in contrast to biotyping, ribotyping with ecori and hindiii restriction endonucleases and whole-cell protein fingerprinting assigned six analysed strains as s. equoru ...200616585638
halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand.microbially induced calcium carbonate precipitation (micp) is a phenomenon based on urease activity of halotolerant and alkaliphilic microorganisms that can be used for the soil bioclogging and biocementation in geotechnical engineering. however, enrichment cultures produced from indigenous soil bacteria cannot be used for large-scale micp because their urease activity decreased with the rate about 5 % per one generation. to ensure stability of urease activity in biocement, halotolerant and alka ...201323529354
draft genome sequence of staphylococcus succinus strain csm-77, a moderately halophilic bacterium isolated from a triassic salt mine.here, we report the draft genome sequence of staphylococcus succinus strain csm-77. this moderately halophilic bacterium was isolated from the surface of a halite sample obtained from a triassic salt mine.201627284152
identification and characterization of novel natural pathogen of drosophila melanogaster isolated from wild captured drosophila spp.drosophila melanogaster is an emerging model system for the study of evolutionary ecology of immunity. however, a large number of studies have used non natural pathogens as very few natural pathogens have been isolated and identified. our aim was to isolate and characterize natural pathogen/s of d. melanogaster. a bacterial pathogen was isolated from wild caught drosophila spp., identified as a new strain of staphylococcus succinus subsp. succinus and named pk-1. this strain induced substantial ...201627492855
molecular diversity and multifarious plant growth promoting attributes of bacilli associated with wheat (triticum aestivum l.) rhizosphere from six diverse agro-ecological zones of india.the diversity of culturable bacilli was investigated in six wheat cultivating agro-ecological zones of india viz: northern hills, north western plains, north eastern plains, central, peninsular, and southern hills. these agro-ecological regions are based on the climatic conditions such as ph, salinity, drought, and temperature. a total of 395 bacilli were isolated by heat enrichment and different growth media. amplified ribosomal dna restriction analysis using three restriction enzymes alui, msp ...201626567901
isolation and characterisation of bacterial colonies from seeds and in vitro cultures of fraxinus spp. from italian sites.culturable bacteria were isolated from seeds, embryos and contaminated in vitro cultures of ash (fraxinus excelsior l., f. ornus l. and f. angustifolia l.) and were identified using morphological and molecular analyses. fourteen morphologically distinct isolates were recovered from seeds of fraxinus spp. 16s rdna sequencing categorised these isolates into ten separate genera. three strains isolated from contaminated in vitro cultures, pantoea agglomerans, staphylococcus succinus and aerococcus v ...201121143738
staphylococcus succinus sp. nov., isolated from dominican amber.two bacterial isolates, designated amg-d1t and amg-d2, were recovered from 25-35-million-year-old dominican amber. amg-d1t and amg-d2 biochemically most closely resemble staphylococcus xylosus; they differ physiologically from other staphylococci. fatty acid analysis and comparisons with extensive databases were unable to show relatedness to any specific taxon. moreover, amg-d1t and amg-d2 contain tuberculostearic acid and meso-diaminopimelic acid, characteristic of the g + c-rich coryneform bac ...19989731292
microbiota of high-pressure-processed serrano ham investigated by culture-dependent and culture-independent methods.the microbiota of serrano dry-cured ham of different chemical composition, subjected or not to high-pressure processing (hpp), was investigated using culture-dependent and culture-independent methods. microbial counts were submitted to analysis of variance with physicochemical parameters (aw, nacl concentration, salt-in-lean ratio and intramuscular fat content) or hpp as main effects. in untreated hams, physicochemical parameters significantly affected counts of aerobic mesophiles, psychrotrophs ...201727835773
dna microarray based detection of genes involved in safety and technologically relevant properties of food associated coagulase-negative staphylococci.aim of the work was to design a polynucleotide based dna microarray as screening tool to detect genes in food associated coagulase-negative staphylococci (cns). a focus was laid on genes with potential health concern and technological relevance. the microarray contained 220 probes for genes encoding antibiotic resistances, hemolysins, toxins, amino acid decarboxylases (e.g. biogenic amine formation), binding proteins to extracellular matrix (ecm), lipases, proteases, stress response factors, or ...201121329998
diversity of microorganisms isolated from amber.> abstract claims that organisms can be cultured from amber, if substantiated, would be significant contributions to our understanding of the evolution, tenacity, and potential spread of life. three reports on the isolation of organisms from amber have been published. cano and borucki recently reported the isolation of bacillus sphaericus and lambert et al. have described a new species designated staphylococcus succinus from 25-40 million year old dominican amber. these characterized organisms w ...199910384010
the application of staphylococci with flavour-generating potential is affected by acidification in fermented dry sausages.differences in the production of bacterial metabolites with potential impact on fermented sausage flavour were found in meat simulation medium when comparing different strains of staphylococcus xylosus and staphylococcus carnosus as starter cultures. overall, higher levels of 3-methyl-1-butanol and acetoin were found for s. xylosus, with some intraspecies variability. in addition, sausage fermentation parameters affected staphylococcal growth and metabolism. strong acidification, as in northern- ...201020688237
staphylococcus succinus subsp. casei subsp. nov., a dominant isolate from a surface ripened cheese.a new subspecies of the species staphylococcus succinus, isolated from a swiss surface ripened cheese, is described. this subspecies is differentiated from the species staphylococcus succinus atcc 700337t on the basis of dna-dna hybridisation, cell wall composition and phenotypic characteristics. staphylococcus succinus subsp. casei could be distinguished among other things by its ability to reduce nitrate, form acid from d-mannose and d-melezitose, ferment adenosine, inosine, d-sorbitol, and 2, ...200212421073
application of different molecular techniques for characterization of catalase-positive cocci isolated from sucuk.this study was carried out for the characterization and discrimination of the indigenous gram positive, catalase-positive cocci (gcc) population in sucuk, a traditional turkish dry-fermented sausage. sucuk samples, produced by the traditional method without starter culture were collected from 8 local producers in kayseri/turkey and a total of 116 gcc isolates were identified by using different molecular techniques. two different molecular fingerprinting methods; namely, randomly amplified polymo ...201424410408
halophilic microorganisms in deteriorated historic buildings: insights into their characteristics.historic buildings are constantly being exposed to numerous climatic changes such as damp and rainwater. water migration into and out of the material's pores can lead to salt precipitation and the so-called efflorescence. the structure of the material may be seriously threatened by salt crystallization. a huge pressure is produced when salt hydrates occupy larger spaces, which leads at the end to cracking, detachment and material loss. halophilic microorganisms have the ability to adapt to high ...201626894235
monitoring of the microbiota of fermented sausages by culture independent rrna-based approaches.in italy, fermented sausages (called "salami") are consumed in large quantities. salami samples from a local meat factory in the area of torino were analyzed at 0, 3, 7, 30 and 45 days of ripening. swab samples from the production environment were also collected at the beginning of the experiment. the diversity of metabolically active microbiota occurring during the natural fermentation of salami was evaluated by using rt-pcr-dgge coupled with rna-based pyrosequencing of the 16s rrna gene. a cul ...201525724303
safety and technological characterization of coagulase-negative staphylococci isolates from traditional korean fermented soybean foods for starter development.to select starters for the production of meju and doenjang, traditional korean fermented soybean foods, we assessed the safety and technological properties of their predominant isolates, staphylococcus saprophyticus, staphylococcus succinus and staphylococcus xylosus. phenotypic antibiotic resistance, hemolysis and biofilm formation were strain-specific. none of the s. succinus isolates exhibited antibiotic resistance or hemolytic activities. thirty-three selected strains, identified through saf ...201627427871
antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures.the resistance of 330 coagulase-negative staphylococci (cns) associated with food or used in starter cultures and belonging to the species staphylococcus carnosus, staphylococcus condimenti, staphylococcus piscifermentans, staphylococcus equorum, staphylococcus succinus and staphylococcus xylosus, against 21 antibiotics was determined using the disk diffusion method. the incidence and number of resistances was found to be species and source of isolation dependent. most strains of s. equorum (63% ...200818625535
sexual activity increases resistance against pseudomonas entomophila in male drosophila melanogaster.maintenance and deployment cost of immunity is high, therefore, it is expected to trade-off with other high cost traits like sexual activity. previous studies with drosophila melanogaster show that male's ability to clear bacteria decreases with increase in sexual activity. we subjected this idea to test using two pathogens (pseudomonas entomophila and staphylococcus succinus) and three different populations of drosophila melanogaster.201324010544
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