| roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop pecten maximus. | four bacterial strains were isolated from larval cultures and collectors of the scallop pecten maximus. they showed a high level of intragroup genomic relatedness (84-95%) as determined by dna-dna hybridization. the cells were gram-negative, strictly aerobic, motile, ovoid rods. they grew at temperatures from 15 to 37 degrees c and from ph 7.0 to 10, but did not grow in the absence of nacl and required growth factors. they had the ability to use a wide variety of compounds as sole carbon source: ... | 1998 | 9731295 |
| seasonal incidence of autochthonous antagonistic roseobacter spp. and vibrionaceae strains in a turbot larva (scophthalmus maximus) rearing system. | bacteria inhibitory to fish larval pathogenic bacteria were isolated from two turbot larva rearing farms over a 1-year period. samples were taken from the rearing site, e.g., tank walls, water, and feed for larvae, and bacteria with antagonistic activity against vibrio anguillarum were isolated using a replica plating assay. approximately 19,000 colonies were replica plated from marine agar plates, and 341 strains were isolated from colonies causing clearing zones in a layer of v. anguillarum. w ... | 2004 | 15574928 |
| competitive interactions in mixed-species biofilms containing the marine bacterium pseudoalteromonas tunicata. | pseudoalteromonas tunicata is a biofilm-forming marine bacterium that is often found in association with the surface of eukaryotic organisms. it produces a range of extracellular inhibitory compounds, including an antibacterial protein (alpp) thought to be beneficial for p. tunicata during competition for space and nutrients on surfaces. as part of our studies on the interactions between p. tunicata and the epiphytic bacterial community on the marine plant ulva lactuca, we investigated the hypot ... | 2005 | 15811995 |
| isolation of aryl-phosphate ester-degrading bacterium from leachate of a sea-based waste disposal site. | an aryl-phosphate ester (ape)-degrading bacterium was isolated from the leachate of a sea-based waste disposal site. the isolated ape-degrading bacterial strain ys-57 grew well in a medium containing glucose and nacl, and degraded two types of ape:tricresyl phosphate and triphenyl phosphate. the optimal temperature, ph, and nacl concentration for the growth of strain ys-57 were 30 degrees c, 7.0, and 1.0%, respectively. strain ys-57 grew at an ape concentration of 25 mg/l without being inhibited ... | 2004 | 16233737 |
| reclassification of roseobacter gallaeciensis ruiz-ponte et al. 1998 as phaeobacter gallaeciensis gen. nov., comb. nov., description of phaeobacter inhibens sp. nov., reclassification of ruegeria algicola (lafay et al. 1995) uchino et al. 1999 as marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera roseobacter, ruegeria and leisingera. | a heterotrophic, antibiotic-producing bacterium, strain t5(t), was isolated from the german wadden sea, located in the southern region of the north sea. sequence analysis of the 16s rrna gene of this strain demonstrated close affiliation with roseobacter gallaeciensis bs107(t) (99 % similarity), but the results of genotypic (dna-dna hybridization and dna g + c content) and phenotypic characterization revealed that strain t5(t) represents a novel species. the novel organism is strictly aerobic, g ... | 2006 | 16738106 |
| microbial colonization and competition on the marine alga ulva australis. | pseudalteromonas tunicata and roseobacter gallaeciensis are biofilm-forming marine bacteria that are often found in association with the surface of the green alga ulva australis. they are thought to benefit the plant host by producing inhibitory compounds that are active against common fouling organisms. we investigated factors that influence the ability of p. tunicata and r. gallaeciensis to attach to and colonize the plant surface and also the competitive interactions that occur between these ... | 2006 | 16885308 |
| effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (litopenaeus vannamei) following immersion challenge with vibrio parahaemolyticus. | four bacterial strains isolated from the gastrointestinal tract of adult shrimp litopenaeus vannamei, vibrio alginolyticus utm 102, bacillus subtilis utm 126, roseobacter gallaeciensis slv03, and pseudomonas aestumarina slv22, were evaluated for potential use as probiotics for shrimp. in vitro studies demonstrated antagonism against the shrimp-pathogenic bacterium, vibrio parahaemolyticus ps-017. feeding shrimp with diets containing the potential probiotics showed the best feed conversion ratio ... | 2007 | 17544437 |
| low densities of epiphytic bacteria from the marine alga ulva australis inhibit settlement of fouling organisms. | bacteria that produce inhibitory compounds on the surface of marine algae are thought to contribute to the defense of the host plant against colonization of fouling organisms. however, the number of bacterial cells necessary to defend against fouling on the plant surface is not known. pseudoalteromonas tunicata and phaeobacter sp. strain 2.10 (formerly roseobacter gallaeciensis) are marine bacteria often found in association with the alga ulva australis and produce a range of extracellular inhib ... | 2007 | 17965210 |
| phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the arctic. | a gram-negative, psychrophilic, motile, rod-shaped bacterium, designated strain 20188(t), was isolated from marine samples collected from the arctic (7 degrees 00' 24'' n 16 degrees 59' 37'' w), and was identified taxonomically by means of a polyphasic study. on the basis of 16s rrna gene sequence similarity, strain 20188(t) was closely related to members of the genera phaeobacter. 16s rrna gene sequence similarities between strain 20188(t) and the type strains of phaeobacter inhibens, phaeobact ... | 2008 | 18523182 |
| phaeobacter and ruegeria species of the roseobacter clade colonize separate niches in a danish turbot (scophthalmus maximus)-rearing farm and antagonize vibrio anguillarum under different growth conditions. | members of the roseobacter clade colonize a spanish turbot larval unit, and one isolate (phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. here, we demonstrate that roseobacters with antagonistic activity against vibrio anguillarum also colonize a danish turbot larval farm that relies on a very different water source (the danish fiord limfjorden as opposed to the galician atlantic ocean). phylogenetic analyses based on 16s rrna and gyrase b gene sequences re ... | 2008 | 18952864 |
| metabolic fluxes in the central carbon metabolism of dinoroseobacter shibae and phaeobacter gallaeciensis, two members of the marine roseobacter clade. | in the present work the central carbon metabolism of dinoroseobacter shibae and phaeobacter gallaeciensis was studied at the level of metabolic fluxes. these two strains belong to the marine roseobacter clade, a dominant bacterial group in various marine habitats, and represent surface-associated, biofilm-forming growth (p. gallaeciensis) and symbiotic growth with eukaryotic algae (d. shibae). based on information from recently sequenced genomes, a rich repertoire of pathways has been identified ... | 2009 | 19788729 |
| genetic tools for the investigation of roseobacter clade bacteria. | the roseobacter clade represents one of the most abundant, metabolically versatile and ecologically important bacterial groups found in marine habitats. a detailed molecular investigation of the regulatory and metabolic networks of these organisms is currently limited for many strains by missing suitable genetic tools. | 2009 | 20021642 |
| identification and biosynthesis of tropone derivatives and sulfur volatiles produced by bacteria of the marine roseobacter clade. | bacteria of the roseobacter clade are abundant marine bacteria and are important contributors to the global sulfur cycle. the volatiles produced by two of its members, phaeobacter gallaeciensis and oceanibulbus indolifex, were analyzed to investigate whether the released compounds are derived from sulfur metabolism, and which biosynthetic pathways are involved in their formation. both bacteria emitted different sulfides and thioesters, including new natural compounds such as s-methyl phenylethan ... | 2010 | 20024154 |
| pathways and substrate specificity of dmsp catabolism in marine bacteria of the roseobacter clade. | the volatiles released by phaeobacter gallaeciensis, oceanibulbus indolifex and dinoroseobacter shibae have been investigated by gc-ms, and several mesh-derived sulfur volatiles have been identified. an important sulfur source in the oceans is the algal metabolite dimethylsulfoniopropionate (dmsp). labelled [2h6]dmsp was fed to the bacteria to investigate the production of volatiles from this compound through the lysis pathway to [2h6]dimethylsulfide or the demethylation pathway to [2h3]-3-(meth ... | 2010 | 20043308 |
| the jekyll-and-hyde chemistry of phaeobacter gallaeciensis. | emiliania huxleyi, an environmentally important marine microalga, has a bloom-and-bust lifestyle in which massive algal blooms appear and fade. phaeobacter gallaeciensis belongs to the roseobacter clade of a-proteobacteria, the populations of which wax and wane with that of e. huxleyi. roseobacter are thought to promote algal growth by biosynthesizing and secreting antibiotics and growth stimulants (auxins). here we show that p. gallaeciensis switches its secreted small molecule metabolism to th ... | 2011 | 21430694 |
| roseobacticides: small molecule modulators of an algal-bacterial symbiosis. | marine bacteria and microalgae engage in dynamic symbioses mediated by small molecules. a recent study of phaeobacter gallaeciensis, a member of the large roseobacter clade of α-proteobacteria, and emiliania huxleyi, a prominent member of the microphytoplankton found in large algal blooms, revealed that an algal senescence signal produced by e. huxleyi elicits the production of novel algaecides, the roseobacticides, from the bacterial symbiont. in this report, the generality of these findings ar ... | 2011 | 21928816 |
| Tropodithietic acid production in Phaeobacter gallaeciensis is regulated by N-acyl homoserine lactone-mediated quorum sensing. | The production of N-acyl homoserine lactones (AHLs) is widely distributed within the marine Roseobacter clade, and it was proposed that AHL-mediated quorum sensing (QS) is one of the most common cell-to-cell communication mechanisms in roseobacters. The traits regulated by AHL-mediated QS are yet not known for members of the Roseobacter clade, but production of the antibiotic tropodithietic acid (TDA) was supposed to be controlled by AHL-mediated QS in Phaeobacter spp. We describe here for the f ... | 2011 | 21949069 |
| marine bacteria from danish coastal waters show antifouling activity against the marine fouling bacterium pseudoalteromonas sp. strain s91 and zoospores of the green alga ulva australis independent of bacteriocidal activity. | the aims of this study were to determine if marine bacteria from danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. we further assess if antibacterial effect is a good proxy for antifouling activity. we isolated 110 bacteria with anti-vibrio activity from different sample types and locations during a 1-year sampling from danish coastal waters. the strains were identified as pseudoalteromonas, phaeobacter, and vibrionac ... | 2011 | 22003011 |
| genome sequence of the phage-gene rich marine phaeobacter arcticus type strain dsm 23566(t.). | phaeobacter arcticus zhang et al. 2008 belongs to the marine roseobacter clade whose members are phylogenetically and physiologically diverse. in contrast to the type species of this genus, phaeobacter gallaeciensis, which is well characterized, relatively little is known about the characteristics of p. arcticus. here, we describe the features of this organism including the annotated high-quality draft genome sequence and highlight some particular traits. the 5,049,232 bp long genome with its 4, ... | 2013 | 24501630 |
| disruption of cell-to-cell signaling does not abolish the antagonism of phaeobacter gallaeciensis toward the fish pathogen vibrio anguillarum in algal systems. | quorum sensing (qs) regulates phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that qs is not important for antagonism in algal cultures. qs mutants reduced vibrio anguillarum to the same extent as the wild type. consequently, a combination of probiotic phaeobacter and qs inhibitors is a feasible strategy for aquaculture disease control. | 2013 | 23811510 |
| phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life. | phaeobacter gallaeciensis, a member of the abundant marine roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. production of the antibiotic tropodithietic acid (tda) makes p. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pathogens. in addition to tda, several other secondary metabolites are produced, allowing the mutualistic bacterium to also act as an opportunistic pathogen. here we provide the manually annotated genom ... | 2012 | 22717884 |
| jindonia aestuariivivens gen. nov., sp. nov., isolated from a tidal flat on the south-western sea in republic of korea. | a gram-stain-negative, aerobic, non-flagellated, and coccoid, ovoid or rod-shaped bacterium, designated jdtf-65(t), was isolated from a tidal flat on the south-western sea in republic of korea. strain jdtf-65(t) grew optimally at 25°c, at ph 7.0-8.0 and in the presence of 2.0% (w/v) nacl. strain jdtf-65(t) exhibited 16s rrna gene sequence similarities of 97.1-97.6% to the type strains of 'aliisedimentitalea scapharcae', phaeobacter gallaeciensis, phaeobacter inhibens, leisingera aquimarina, trop ... | 2017 | 28251548 |
| a comprehensive analysis of the microbial communities of healthy and diseased marine macroalgae and the detection of known and potential bacterial pathogens. | microorganisms are increasingly being recognized as the causative agents in the diseases of marine higher organisms, such as corals, sponges, and macroalgae. delisea pulchra is a common, temperate red macroalga, which suffers from a bleaching disease. two bacterial strains, nautella italica r11 and phaeobacter gallaeciensis lss9, have been shown in vitro to cause bleaching symptoms, but previous work has failed to detect them during a natural bleaching event. to provide a link between in vitro o ... | 2015 | 25759688 |
| complete genome sequence of the phaeobacter gallaeciensis type strain cip 105210(t) (= dsm 26640(t) = bs107(t)). | phaeobacter gallaeciensis cip 105210(t) (= dsm 26640(t) = bs107(t)) is the type strain of the species phaeobacter gallaeciensis. the genus phaeobacter belongs to the marine roseobacter group (rhodobacteraceae, alphaproteobacteria). phaeobacter species are effective colonizers of marine surfaces, including frequent associations with eukaryotes. strain bs107(t) was isolated from a rearing of the scallop pecten maximus. here we describe the features of this organism, together with the complete geno ... | 2014 | 25197473 |
| cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus cribrihabitans chen et al. 2014. | a gram-stain-negative, strictly aerobic, rod- or oval-shaped, motile, non-spore-forming bacterium, designated strain cc-amhb-3(t), was isolated from coastal surface seawater off hualien, taiwan. the novel strain showed high pairwise 16s rrna gene sequence similarities to ruegeria mobilis nbrc 101030(t) (96.5%), ruegeria scottomollicae lmg 24367(t) (96.4%), phaeobacter aquaemixtae ssk6-1(t) (96.2%), phaeobacter daeponensis tf-218(t) (96.2%), cribrihabitans marinus cz-am5(t) (96.1%) and other spec ... | 2014 | 25180090 |
| seohaeicola nanhaiensis sp. nov., a moderately halophilic bacterium isolated from the benthic sediment of south china sea. | an aerobic, gram-staining negative, non-motile, and rod-shaped bacterial strain, ss011a0-7#2-2(t), was isolated from the sediment of south china sea with the depth of 1,500 m. optimum growth occurred at ph 8.0, 30 °c, and 6 % (w/v) nacl. strain ss011a0-7#2-2(t) did not synthesize bacteriochlorophyll a or carotenoid, neither possess photosynthesis genes. its genome dna g+c content was 67.9 mol%. it contained q-10 as the predominant ubiquinone and c18:1 ω7c (52.3 %) as the major fatty acid. the ma ... | 2014 | 25027448 |
| description of tropicibacter mediterraneus sp. nov. and tropicibacter litoreus sp. nov. | four strains (m15∅_3, m17(t), m49 and r37(t)) were isolated from mediterranean seawater at malvarrosa beach, valencia, spain. together with an older preserved isolate (strain 2om6) from cultured oysters at vinaroz, castellón, spain, the strains were thoroughly characterized in a polyphasic study and were placed phylogenetically within the roseobacter clade in the family rhodobacteraceae. highest 16s rrna sequence similarities of the five strains to the types of any established species correspond ... | 2013 | 23706913 |
| phaeobacter gallaeciensis reduces vibrio anguillarum in cultures of microalgae and rotifers, and prevents vibriosis in cod larvae. | phaeobacter gallaeciensis can antagonize fish-pathogenic bacteria in vitro, and the purpose of this study was to evaluate the organism as a probiont for marine fish larvae and their feed cultures. an in vivo mechanism of action of the antagonistic probiotic bacterium is suggested using a non-antagonistic mutant. p. gallaeciensis was readily established in axenic cultures of the two microalgae tetraselmis suecica and nannochloropsis oculata, and of the rotifer brachionus plicatilis. p. gallaecien ... | 2012 | 22928051 |