Publications

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genetic variation of symbiotic fungi cultivated by the macrotermitine termite odontotermes formosanus (isoptera: termitidae) in the ryukyu archipelago.fungus-growing termites have a mutualistic relationship with their cultivated fungi. to improve understanding of genetic aspects of this relationship, we examined molecular markers in the fungus-growing termite odontotermes formosanus and its fungi termitomyces spp. from the ryukyu archipelago. based on the polymorphic band patterns obtained from arbitrarily primed polymerase chain reaction methods, we constructed cladograms for related colonies of the termites and fungi. the resulting trees ind ...200212144675
termite-regulated fungal monoculture in fungus combs of a macrotermitine termite odontotermes formosanus.the mechanism of the exclusive growth of termitomyces in fungus combs with fungi-growing termites, o. formosanus was examined using laboratory scale fungus combs. in the combs without the termites, vigorous growth of unidentified fungi was observed although no significant change was found in the case of the combs with termites. in addition, these results were reproducible even when incubated in a separated dish, suggesting that the physicochemical conditions were not the reason for the growth. w ...200516141705
characterization of a newly discovered china variety of metarhizium anisopliae (m. anisopliae var. dcjhyium) for virulence to termites, isoenzyme, and phylogenic analysis.the efficacy of a new virulent metarhizium anisopliae variety (m. anisopliae var. dcjhyium, dq288247) obtained from odontotermes formosanus in china was evaluated against the subterranean termite, o. formosanus, in the laboratory. the new variety was compared with four other virulent m. anisopliae isolates and was found to be highly infectious and virulent against termites. m. anisopliae var. dcjhyium could cause approximately 100% mortality of termites 3 days post-inoculation in the concentrati ...200716949807
trabulsiella odontotermitis sp. nov., isolated from the gut of the termite odontotermes formosanus shiraki.bacterial strains eant 3-9(t) and eant 3-3, isolated from the gut of the termite odontotermes formosanus, were characterized using a polyphasic taxonomic approach. cells of these organisms were gram-negative, non-pigmented, rod-shaped, non-endospore-forming and facultatively anaerobic. phenotypic, physiological and genetic characteristics of the two strains were highly similar. phylogenetic analyses using 16s rrna gene sequences showed that strain eant 3-9(t) formed a monophyletic branch towards ...200717392189
comamonas odontotermitis sp. nov., isolated from the gut of the termite odontotermes formosanus.a bacterial strain, designated dant 3-8(t), isolated from the gut of the termite odontotermes formosanus, was investigated by a polyphasic taxonomic approach. the cells were rod-shaped, gram-negative, non-pigmented, non-spore-forming and non-fermentative. phylogenetic analyses using the 16s rrna gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus comamonas, its closest neighbours being comamonas testosteroni ...200717392226
pathogenicity of a new china variety of metarhizium anisopliae (m. anisopliae var. dcjhyium) to subterranean termite odontotermes formosanus.the pathogenicity of a new china variety of metarhizium anisopliae (m. anisopliae var. dcjhyium) against the subterranean termite odontotermes formosanus and the effect of the fungal fermented solution on hemolymph intracellular calcium were studied in laboratory. conidia from the m. anisopliae var. dcjhyium were highly virulent for o. formosanus causing approximately 100% mortality 3 days post-inoculation in the concentration of 3x10(8) conidia/ml. the conidial clumps with conidial chains distr ...200917482440
new acrylamide and oxazolidin derivatives from a termite-associated streptomyces sp.two new compounds, named 2-formylpyrrole-4-acrylamide (1) and dihydrostreptazolin (2) were isolated from the fermentation broth of by-4, an actinomycetes residing in the gut of odontotermes formosanus. the structures of 1 and 2 were elucidated by extensive spectral analysis (1h, 13c, 2d nmr, and hr-esims). the isolated compounds were assayed for cytotoxic and antimicrobial activities.201121485273
effect of wood hardness and secondary compounds on feeding preference of odontotermes formosanus (isoptera: termitidae).odontotermes formosanus (shiraki) (isoptera: termitidae) is one of the most destructive plant pests in china, which control relies mainly on baits strategies. baits made from the wood of eight different tree species were used to study the feeding preference of this termite, and conversely wood protection strategies of the tree species. three bait types were used to identify wood protection strategies: solid wood (physical and chemical protection), crude flour (chemical protection) made from grou ...201121735905
Microbial community analysis in the termite gut and fungus comb of Odontotermes formosanus: the implication of Bacillus as mutualists.The microbial communities harbored in the gut and fungus comb of the fungus-growing termite Odontotermes formosanus were analyzed by both culture-dependent and culture-independent methods to better understand the community structure of their microflora. The microorganisms detected by denaturing gradient gel electrophoresis (DGGE), clonal selection, and culture-dependent methods were hypothesized to contribute to cellulose-hemicellulose hydrolysis, gut fermentation, nutrient production, the break ...201122092951
chroniodiplogaster formosiana sp. n. (rhabditida: diplogastridae) from chinese populations of odontotermes formosanus shiraki (isoptera: termitidae).chroniodiplogaster formosiana sp. n. (rhabditida: diplogastridae) is described from a population of odontotermes formosanus shiraki (isoptera: termitidae) collected from dead (unidentified) wood at dinghu park, china. characters of c. formosiana which separate it from c. aerivora (cobb) are a longer tail, the presence of four micropapillae (quadriform condition), and the adjacent position of the second and third macropapillae on the male tail. the diplogasterid pristionchus uniformis fedorko & s ...200619259445
naturalization and control of coptotermes gestroi (blattodea: rhinotermitidae) in a taiwanese forest.coptotermes gestroi(wasmann), an invasive termite species in taiwan, has been a major structural pest in southwestern taiwan.c. gestroiwas recently reported to have infested living trees in a taiwanese forest, showing its potential of becoming an invasive forest pest in taiwan. to determine whetherc. gestroihave naturalized in the forests, we monitored their dispersal flights and estimated their colony development status on the basis of their worker and soldier morphology. the results showed tha ...201627016598
vertical distribution of termites on trees in two forest landscapes in taiwan.termites are a key functional group in the forest ecosystem, but they damage trees. to investigate the termite infestation pattern on the whole tree, we cut 108 blackboard trees,alstonia scholaris(l.) r. br., and 50 japanese cedars,cryptomeria japonica (l. f.) d. don, into sections. the bark surface and cross sections of the tree trunk were examined along the axes. a high percentage of blackboard trees (92.6%) was infested by fungus-growing termites,odontotermes formosanus(shiraki), but damage w ...201627016004
phylogenetic diversity of archaea in the intestinal tract of termites from different lineages.termites are among the few arthropods that emit methane to the atmosphere, which is a significant source of global greenhouse gas due to their huge biomass on earth. in this study, phylogenetic diversity of archaea of five termite species from different lineages were analyzed based on 16s rrna genes. archaea associated with wood-feeding lower termite, r. chinensis were exclusively methanobrevibacter in the order methanobacteriales. this type of methanogens was also found in nasutitermes sp. and ...201525709053
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