| ornibactin production and transport properties in strains of burkholderia vietnamiensis and burkholderia cepacia (formerly pseudomonas cepacia). | several strains of burkholderia vietnamiensis, isolated from the rhizosphere of rice plants, and four strains formerly known as pseudomonas cepacia including two collection strains and two clinical isolates were compared for siderophore production and iron uptake. the b. vietnamiensis (tvv strains) as well as the b. cepacia strains (atcc 25416 and atcc 17759) and the clinical isolates k132 and lmg 6999 were all found to produce ornibactins under iron starvation. the two atcc strains of b. cepaci ... | 1995 | 7580051 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |
| development of rrna-based pcr assays for identification of burkholderia cepacia complex isolates recovered from cystic fibrosis patients. | pcr assays targeting rrna genes were developed to identify species (genomovars) within the burkholderia cepacia complex. each assay was tested with 177 bacterial isolates that also underwent taxonomic analysis by whole-cell protein profile. these isolates were from clinical and environmental sources and included 107 b. cepacia complex strains, 23 burkholderia gladioli strains, 20 ralstonia pickettii strains, 10 pseudomonas aeruginosa strains, 8 stenotrophomonas maltophilia strains, and 9 isolate ... | 1999 | 10488171 |
| burkholderia uboniae sp. nov., l-arabinose-assimilating but different from burkholderia thailandensis and burkholderia vietnamiensis. | a polar multitrichous gram-negative motile rod, ey 3383, originally identified as burkholderia thailandensis, revealed a dna-dna reassociation rate of 36.7%, under stringent conditions, with the type strain of b. thailandensis, despite the 16s rdna homology value between two type strains being as high as 97.9%. the strain was clearly differentiated from the type strain of b. thailandensis by physiological, bio-chemical, and nutritional characteristics, without significant difference in cellular ... | 2000 | 10832977 |
| effects of rice seed surface sterilization with hypochlorite on inoculated burkholderia vietnamiensis. | when a combination of hydrogen peroxide and hypochlorite was used to surface sterilize rice seeds, a 10(2)- to 10(4)-fold decrease in cfu was observed during the first 15 h after inoculation of the rice rhizosphere organism burkholderia vietnamiensis tvv75. this artifact could not be eliminated simply by rinsing the seeds, even thoroughly, with sterile distilled water. when growth resumed, a significant increase in the frequency of rifampin- and nalidixic acid-resistant mutants in the population ... | 2001 | 11425720 |
| detection and activity of insertion sequences in environmental strains of burkholderia. | the presence of two insertion sequences, is406 and is407, was tested by polymerase chain reaction (pcr) amplification in 25 strains representing 15 burkholderia species and the close relative ralstonia pickettii. a total of 50% of the 25 strains contained at least one of the two insertion sequences (iss) and a statistically significant correlation was found between the occurrences of is406 and is407. moreover, pcr-rflp studies of the amplified fragments showed that is406 is largely conserved amo ... | 2001 | 11846770 |
| use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. | during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ... | 2002 | 12354883 |
| genomovar diversity amongst burkholderia cepacia complex isolates from an australian adult cystic fibrosis unit. | in this study, a combination of reca-based pcr assays and 16s rdna restriction fragment length polymorphism (rflp) analysis was used to determine the genomovar diversity of clinical burkholderia cepacia complex isolates. twenty-eight isolates were prospectively collected from patients attending a large australian adult cystic fibrosis (cf) unit, 22 isolates were referred from other australian cf units and a further eight isolates originated from patients without cf. the 28 prospectively collecte ... | 2003 | 12884074 |
| polyclonal outbreak of burkholderia cepacia complex bacteraemia in haemodialysis patients. | we report a polyclonal outbreak of bacteraemia involving 24 patients at a haemodialysis facility in recife (brazil). during the outbreak period (4 june to 11 july, 2001), three burkholderia cepacia complex strains were isolated from human blood and from various water samples collected at different sites in the haemodialysis unit and from dialysate fluids. out of 14 patients with positive blood cultures, six were infected by burkholderia cepacia complex bacteria: three with burkholderia cepacia g ... | 2003 | 12818585 |
| dna-dna hybridization study of burkholderia species using genomic dna macro-array analysis coupled to reverse genome probing. | the present study was aimed at simplifying procedures to delineate species and identify isolates based on dna-dna reassociation. dna macro-arrays harbouring genomic dna of reference strains of several burkholderia species were produced. labelled genomic dna, hybridized to such an array, allowed multiple relative pairwise comparisons. based on the relative dna-dna relatedness values, a complete data matrix was constructed and the ability of the method to discriminate strains belonging to differen ... | 2003 | 12807195 |
| suppression-subtractive hybridisation reveals variations in gene distribution amongst the burkholderia cepacia complex, including the presence in some strains of a genomic island containing putative polysaccharide production genes. | some strains of the burkholderia cepacia complex, including the et12 lineage, have been implicated in epidemic spread amongst cystic fibrosis (cf) patients. suppression-subtractive hybridisation was used to identify genomic regions within strain j2315 (et12 lineage; genomovar iiia) that were absent from a non-transmissible genomovar iiib strain. sequence data from 15 subtracted clones were used to interrogate the genome sequence of strain j2315 and identify genomic regions incorporating the subt ... | 2003 | 12610727 |
| cloning and characterization of the burkholderia vietnamiensis norm gene encoding a multi-drug efflux protein. | polymyxin b-sensitive mutants in burkholderia vietnamiensis (burkholderia cepacia genomovar v) were generated with a mini-tn5 encoding tetracycline resistance. one of the transposon mutants had an insertion in the norm gene encoding a multi-drug efflux protein. expression of b. vietnamiensis norm in an escherichia coli acrab deletion mutant complemented its norfloxacin hypersensitivity, indicating that the protein functions in drug efflux. however, no effect on antibiotic sensitivity other than ... | 2002 | 12399047 |
| evaluation of restriction fragment length polymorphism analysis of 16s rdna as a tool for genomovar characterisation within the burkholderia cepacia complex. | a total of 154 burkholderia cepacia complex strains, isolated from cystic fibrosis and non-cystic fibrosis patients and the environment, representing all nine genomovars and a putative tenth, were analysed by 16s rdna-restriction fragment length polymorphism using the restriction enzymes alui, cfoi and ddei. examining this diverse strain collection resulted in very diverse restriction patterns. only b. cepacia genomovar vi could be identified unambiguously. the same restriction patterns were obs ... | 2002 | 12204364 |
| identification of a general secretory pathway in a human isolate of burkholderia vietnamiensis (formerly b. cepacia complex genomovar v) that is required for the secretion of hemolysin and phospholipase c activities. | members of the burkholderia cepacia complex can secrete proteases, lipases, and hemolysins. we report in this study the identification of a general secretory pathway present in a b. vietnamiensis (formerly genomovar v) clinical isolate, which is required for the efficient secretion of phospholipase c and hemolysin activities. southern blot hybridization experiments revealed that this general secretion pathway is highly conserved among the different genomovars of the b. cepacia complex and is hom ... | 2002 | 12071681 |
| misidentification of a genomovar of burkholderia cepacia by reca restriction fragment length polymorphism. | an 8 year old girl with cystic fibrosis presented with a pulmonary exacerbation from which burkholderia cepacia was cultured. subsequent polymerase chain reaction restriction fragment length polymorphism analysis of the reca gene suggested the presence of b cepacia genomovar v (burkholderia vietnamiensis); however, on subsequent sequence typing, this isolate was confirmed as b cepacia genomovar iiib. this report outlines the potential difficulties in the correct characterisation of the various g ... | 2002 | 11919219 |
| quorum-sensing signals and quorum-sensing genes in burkholderia vietnamiensis. | acyl-homoserine lactone (acyl-hsl) quorum sensing is common to many proteobacteria including a clinical isolate of burkholderia cepacia. the b. cepacia isolate produces low levels of octanoyl-hsl. we have examined an environmental isolate of burkholderia vietnamiensis. this isolate produced several acyl-hsls. the most abundant species was decanoyl-hsl. decanoyl-hsl in b. vietnamiensis cultures reached concentrations in excess of 20 microm. we isolated a b. vietnamiensis dna fragment containing a ... | 2002 | 11807080 |
| differentiation of species combined into the burkholderia cepacia complex and related taxa on the basis of their fatty acid patterns. | using the established commercial system sherlock (midi, inc.), cellular fatty acid methyl ester analysis for differentiation among burkholderia cepacia complex species was proven. the identification key based on the diagnostic fatty acids is able to discern phenotypically related ralstonia pickettii and pandoraea spp. and further distinguish burkholderia pyrrocinia, burkholderia ambifaria, and burkholderia vietnamiensis. | 2006 | 16517920 |
| pcr-based detection and identification of burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients. | pcr amplification of the reca gene followed by restriction fragment length polymorphism (rflp) analysis was investigated for the rapid detection and identification of burkholderia cepacia complex genomovars directly from sputum. successful amplification of the b. cepacia complex reca gene from cystic fibrosis (cf) patient sputum samples containing b. cepacia genomovar i, burkholderia multivorans, b. cepacia genomovar iii, burkholderia stabilis, and burkholderia vietnamiensis was demonstrated. in ... | 2001 | 11724828 |
| burkholderia ambifaria sp. nov., a novel member of the burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. | a polyphasic taxonomic study, including amplified fragment length polymorphism (aflp) fingerprinting, dna-dna hybridizations, dna base-ratio determinations, phylogenetic analysis, whole-cell fatty acid analyses and an extensive biochemical characterization, was performed on 19 burkholderia cepacia-like isolates from the environment and cystic fibrosis (cf) patients. several of the environmental isolates have attracted considerable interest due to their biocontrol properties. the polyphasic taxon ... | 2001 | 11491349 |
| groel encodes a highly antigenic protein in burkholderia pseudomallei. | no recombinant protein is available for serodiagnosis of melioidosis. in this study, we report the cloning of the groel gene, which encodes an immunogenic protein of burkholderia pseudomallei. bidirectional dna sequencing of groel revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kda. basic local alignment search tool analysis showed that the putative protein encoded by groel is homologous to the chaperonins enco ... | 2001 | 11427437 |
| [burkholderia cepacia: dangers of a phytopathogen organism for patients with cystic fibrosis]. | burkholderia cepacia is an environmental bacterium, capable of colonising vegetal and animal tissues, involved in human opportunist nosocomial infections, and above all, in pulmonary colonisations in patients with cystic fibrosis. in these patients, infection may be followed by a severe deterioration with bacteraemia, leading to death. moreover, owing to the epidemic spread of some clones within cystic fibrosis communities, strict preventive guidelines have to be instituted. early detection of b ... | 2001 | 11397673 |
| burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. | the genus burkholderia comprises 19 species, including burkholderia vietnamiensis which is the only known n(2)-fixing species of this bacterial genus. the first isolates of b. vietnamiensis were recovered from the rhizosphere of rice plants grown in a phytotron, but its existence in natural environments and its geographic distribution were not reported. in the present study, most n(2)-fixing isolates recovered from the environment of field-grown maize and coffee plants cultivated in widely separ ... | 2001 | 11375196 |
| burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in italy. | burkholderia cepacia is a 'complex' in which seven genomic species or genomovars have so far been identified. it appears that all seven b. cepacia genomovars are capable of causing infections in vulnerable persons; in particular, the importance of burkholderia multivorans (genomovar ii) and b. cepacia genomovar iii among cystic fibrosis isolates, especially epidemic ones, has been emphasized. in order to acquire a better comprehension of the genomovar composition of environmental populations of ... | 2001 | 11321544 |
| burkholderia cepacia genomovar vi, a new member of the burkholderia cepacia complex isolated from cystic fibrosis patients. | a polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (cf) patients in the usa. these strains were tentatively identified as burkholderia cepacia, burkholderia vietnamiensis and burkholderia or ralstonia sp. using biochemical tests and 16s rdna-based pcr assays. visual comparison of protein profiles indicated that they belonged to a single new group ('group 13'). the polyphasic taxonomic data showed that 18 of these strains represent a new member of the b. cepaci ... | 2001 | 11321071 |
| outbreak of subclinical mastitis in a flock of dairy sheep associated with burkholderia cepacia complex infection. | an outbreak of subclinical mastitis in a flock of 620 milking sheep was investigated. microbiological and epidemiological analyses identified the causative agent as belonging to the burkholderia cepacia complex (formerly pseudomonas cepacia). every ewe in the milking flock was individually tested for subclinical mastitis on two separate occasions, 6 weeks apart, by the california (rapid) mastitis test (cmt). the proportion of cmt-positive ewes was 69 of 393 (17.6%) on the first sampling and 27 o ... | 2001 | 11230416 |
| dna-based diagnostic approaches for identification of burkholderia cepacia complex, burkholderia vietnamiensis, burkholderia multivorans, burkholderia stabilis, and burkholderia cepacia genomovars i and iii. | bacteria of the burkholderia cepacia complex consist of five discrete genomic species, including genomovars i and iii and three new species: burkholderia multivorans (formerly genomovar ii), burkholderia stabilis (formerly genomovar iv), and burkholderia vietnamiensis (formerly genomovar v). strains of all five genomovars are capable of causing opportunistic human infection, and microbiological identification of these closely related species is difficult. the 16s rrna gene (16s rdna) and reca ge ... | 2000 | 10970351 |
| molecular characterization of burkholderia strains isolated from rice cultivars (oryza sativa l.) for species identification and phylogenetic grouping. | the genus burkholderia consists of extremely versatile bacteria that occupy diverse niches and are commonly encountered in the rhizosphere of crop plants. in this study, we characterized three plant growth promoting strains assigned as burkholderia sp. using biochemical and molecular characterization. the burkholderia spp. strains cbmb40, cbpb-him, and cbpb-hod were characterized using biochemical tests, biolog carbon substrate utilization, fatty acid methyl ester analysis, analysis of reca gene ... | 2008 | 18600039 |
| diagnostically and experimentally useful panel of strains from the burkholderia cepacia complex. | two new species, burkholderia multivorans and burkholderia vietnamiensis, and three genomovars (genomovars i, iii, and iv) currently constitute the burkholderia cepacia complex. a panel of 30 well-characterized strains representative of each genomovar and new species was assembled to assist with identification, epidemiological analysis, and virulence studies on this important group of opportunistic pathogens. | 2000 | 10655415 |
| intracellular survival of burkholderia cepacia complex isolates in the presence of macrophage cell activation. | strains of the burkholderia cepacia complex have emerged as a serious threat to patients with cystic fibrosis due to their ability to infect the lung and cause, in some patients, a necrotizing pneumonia that is often lethal. it has recently been shown that several strains of the b. cepacia complex can escape intracellular killing by free-living amoebae following phagocytosis. in this work, the ability of two b. cepacia complex strains to resist killing by macrophages was explored. using fluoresc ... | 1999 | 10627044 |
| discrimination of burkholderia multivorans and burkholderia vietnamiensis from burkholderia cepacia genomovars i, iii, and iv by pcr. | we present a pcr procedure for identification of burkholderia cepacia, burkholderia multivorans, and burkholderia vietnamiensis. 16s and 23s ribosomal dnas (rdnas) of b. multivorans and b. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. specific antisense 16s rdna primers (for b. cepacia, 5'-agc act ccc rcc tct cag-3'; for b. multivorans, 5'-agc act ccc gaa tct ctt-3') and 23s rdna primers (for b. vietnamiensis, ... | 1999 | 10203482 |
| structural characterisation of a rhamnan and a fucorhamnan, both present in the lipopolysaccharide of burkholderia vietnamiensis strain lmg 10926. | the polymeric fraction isolated after mild acid hydrolysis of the lipopolysaccharide (lps) from burkholderia vietnamiensis strain lmg 10926 contained l-rhamnose (rha) and d-fucose (fuc). from nmr studies supported by the results of methylation analysis and smith degradation, it could be inferred that the material was probably a mixture of two glycans. one component was a linear rhamnan with a trisaccharide repeating unit (1); the other was a branched fucorhamnan with a tetrasaccharide repeating ... | 1998 | 9874236 |
| lipopolysaccharide from burkholderia vietnamiensis strain lmg 6999 contains two polymers identical to those present in the reference strain for burkholderia cepacia serogroup o4. | lipopolysaccharide was isolated from strain lmg 6999 of burkholderia vietnamiensis. degradative and nmr spectroscopic studies established the presence of two polymeric fractions based on the following trisaccharide repeating units: i:-->3)-alpha-d-galp-(1-->3)-beta-d-galp-(1-->3)-beta-d-galpnac- (1-->; ii:-->3)-alpha-d-galpnac-(1-->3)-beta-d-galpnac-(1-->4)- alpha-l-rhap-(1-->. the same polymers have previously been found together in lipopolysaccharide from the reference strain for burkholderia ... | 1997 | 9418254 |
| occurrence of multiple genomovars of burkholderia cepacia in cystic fibrosis patients and proposal of burkholderia multivorans sp. nov. | we performed an integrated genotypic and phenotypic analysis of 128 strains of the genera burkholderia, ralstonia, and pseudomonas in order to study the taxonomic structure of burkholderia cepacia and its relationships with other burkholderia species. our data show that presumed b. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as burkholderia vietnamiensis. this group of five phenotypically similar species is ... | 1997 | 9336927 |
| structure of the o-specific polysaccharide from burkholderia vietnamiensis strain lmg 6998. | the putative o-specific polymer containing d-mannose and l-rhamnose was isolated from the lipopolysaccharide obtained from cells walls of burkholderia vietnamiensis strain lmg 6998. nmr and degradative studies showed that the polymer has a linear trisaccharide repeating-unit of the structure shown. the same polymer carrying an o-acetyl group at position 3 of the 4-substituted mannose residue has previously been found as the o antigen in the related species burkholderia cepacia serogroup j. [form ... | 1996 | 9002192 |
| epidemiology of burkholderia cepacia complex species recovered from cystic fibrosis patients: issues related to patient segregation. | studies of the prevalence of burkholderia cepacia complex species amongst cystic fibrosis (cf) patients in different geographical regions, and the association between cross-infection and putative transmissibility markers, will further our understanding of these organisms and help to address infection-control issues. in this study, b. cepacia complex isolates from cf patients in different regions of europe were analysed. isolates were examined for b. cepacia complex species and putative transmiss ... | 2004 | 15184539 |
| rapid solubilization of insoluble phosphate by a novel environmental stress-tolerant burkholderia vietnamiensis m6 isolated from ginseng rhizospheric soil. | we isolated and characterized novel insoluble phosphate (p)-solubilizing bacteria tolerant to environmental factors like high salt, low and high phs, and low temperature. a bacterium m6 was isolated from a ginseng rhizospheric soil and confirmed to belong to burkholderia vietnamiensis by biolog system and 16s rrna gene analysis. the optimal cultural conditions for the solubilization of p were 2.5% (w/v) glucose, 0.015% (w/v) urea, and 0.4% (w/v) mgcl(2).6h(2)o along with initial ph 7.0 at 35 deg ... | 2010 | 20024543 |
| pcr-based identification and characterization of burkholderia cepacia complex bacteria from clinical and environmental sources. | to study the genotypic identification and characterization of the 119 burkholderia cepacia complex (bcc) strains recovered from clinical and environmental sources in japan and thailand. | 2004 | 15482431 |
| [diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-vetiver zizanioides]. | vetiver zizanioides is a perennial grass of the poaceae family, known of its silage, soil and water conservation role. the aim of the study was to collect and identify the resources of the nitrogen-fixing bacteria associated with vetiver zizanioides. | 2009 | 20112669 |
| [phenotypic and genotypic characteristics of non fermenting atypical strains recovered from cystic fibrosis patients]. | we used partial 16s rrna gene (16s dna) sequencing for the prospective identification of nonfermenting gram-negative bacilli recovered from patients attending our cystic fibrosis center (hôpital necker-enfants malades), which gave problematic results with conventional phenotypic tests. during 1999, we recovered 1093 isolates of nonfermenting gram-negative bacilli from 702 sputum sampled from 148 patients. forty-six of these isolates (27 patients) were not identified satisfactorily in routine lab ... | 2003 | 12948761 |
| improved flagellin genotyping in the burkholderia cepacia complex. | oligonucleotide primers designed to n- and c-terminal sequences of burkholderia cepacia complex flic genes were used to amplify and sequence flic genes from a strain of burkholderia vietnamiensis and three isolates of burkholderia multivorans with large flic genes. alignments incorporating the new sequences enabled the design of polymerase chain reaction (pcr) primers for extension of a published pcr/restriction fragment length polymorphism typing method, to include isolates that previously fail ... | 2003 | 14659536 |
| burkholderia cepacia genomovar iii and burkholderia vietnamiensis double infection in a cystic fibrosis child. | herein we report a case of a cystic fibrosis child who was simultaneously infected with burkholderia cepacia genomovar iii and burkholderia vietnamiensis. after antimicrobial therapy only b. cepacia genomovar iii persisted. | 2002 | 15463830 |
| detection of cultured and uncultured burkholderia cepacia complex bacteria naturally occurring in the maize rhizosphere. | the species composition of a burkholderia cepacia complex population naturally occurring in the maize rhizosphere was investigated by using both culture-dependent and culture-independent methods. b. cepacia complex isolates were recovered from maize root slurry on the two selective media pseudomonas cepacia azelaic acid tryptamine (pcat) and trypan blue tetracycline (tb-t) and subjected to identification by a combination of restriction fragment length polymorphism (rflp) analysis and species-spe ... | 2005 | 16232288 |
| transient isolation of burkholderia multivorans and burkholderia cenocepacia from a brazilian cystic fibrosis patient chronically colonized with burkholderia vietnamiensis. | fifteen serial burkholderia cepacia complex isolates recovered over a period of 4 years from a single cystic fibrosis patient were analysed for genomovar status by means of reca sequence determination, and genetic relatedness by rapd-pcr. twelve isolates were assigned as burkholderia vietnamiensis, two as burkholderia cenocepacia and one as burkholderia multivorans. b. vietnamiensis persisted in the airways during 4 years, except in three occasions when b. cenocepacia or b. multivorans were isol ... | 2005 | 16266831 |
| culture-independent analysis of bacterial fuel contamination provides insights into the level of concordance with the standard industry practice of aerobic cultivation. | bacterial diversity in contaminated fuels has not been systematically investigated using cultivation-independent methods. the fuel industry relies on phenotypic cultivation-based contaminant identification, which may lack accuracy and neglect difficult-to-culture taxa. using industry practice aerobic cultivation, 16s rrna gene sequencing and strain genotyping, a collection of 152 unique contaminant isolates from 54 fuel samples was assembled, and demonstrated a dominance of pseudomonas (21%), bu ... | 2011 | 21602386 |
| distribution and genomic location of active insertion sequences in the burkholderia cepacia complex. | this study aimed firstly to establish the distribution and copy number within the burkholderia cepacia complex of three insertion sequences (is402, is407 and is1416) that possess the ability to activate transcription and hence influence gene expression. a second aim was to map the genomic insertion sites of one of the active insertion sequences (is407) to establish putative links between insertion site and downstream gene activation. the resulting data revealed that all three insertion sequences ... | 2006 | 16388024 |
| identification of burkholderia cepacia complex pathogens by rapid-cycle pcr with fluorescent hybridization probes. | members of the burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (cf) patients. the b. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. in this study, a new approach was developed for the rapid identification of b. cepacia genomovar i, burkholderia multivorans (genomovar ii), burkholderia cenocepacia (lineage iii-a and iii-b), burkholderia stab ... | 2006 | 16687590 |
| spatially-correlated mass spectrometric analysis of microbe--mineral interactions. | a new methodology for examining the interactions of microbes with heterogeneous minerals is presented. imaging laser desorption fourier transform mass spectrometry was used to examine the colonization patterns of burkholderia vietnamiensis g4 (previously burkholderia cepacia g4) on a heterogeneous basalt sample. depth-profile imaging found that the bacterium preferentially colonized the plagioclase mineral phase within the basalt. | 2006 | 16815575 |
| characterization of the poly-β-1,6-n-acetylglucosamine polysaccharide component of burkholderia biofilms. | we demonstrated the production of poly-β-1,6-n-acetylglucosamine (pnag) polysaccharide in the biofilms of burkholderia multivorans, burkholderia vietnamiensis, burkholderia ambifaria, burkholderia cepacia, and burkholderia cenocepacia using an immunoblot assay for pnag. these results were confirmed by further studies, which showed that the pnag hydrolase, dispersin b, eliminated immunoreactivity of extracts from the species that were tested (b. cenocepacia and b. multivorans). dispersin b also i ... | 2011 | 21984237 |
| diversity and distribution of burkholderia cepacia complex in the rhizosphere of rice and maize. | a survey of burkholderia cepacia complex (bcc) species was conducted in agricultural fields within hangzhou, china. out of the 251 bacterial isolates recovered on the selective media from the rhizosphere of rice and maize, 112 of them were assigned to bcc by pcr assays. the species composition of the bcc isolates was analyzed by a combination of reca-restriction fragment length polymorphism assays, species-specific pcr tests and reca gene sequencing. the results revealed that the majority belong ... | 2007 | 17233735 |
| expression of the bviir and cepir quorum-sensing systems of burkholderia vietnamiensis. | burkholderia vietnamiensis has both the cepir quorum-sensing system that is widely distributed among the burkholderia cepacia complex (bcc) and the bviir system. comparison of the expression of cepi, cepr, bvii, and bvir-luxcdabe fusions in b. vietnamiensis g4 and the g4 cepr and bvir mutants determined that the expression of bvii requires both a functional cognate regulator, bvir, and functional cepr. the cepir system, however, is not regulated by bvir. unlike the cepir genes in other bcc speci ... | 2007 | 17277056 |
| identifying the genetic basis of ecologically and biotechnologically useful functions of the bacterium burkholderia vietnamiensis. | signature-tagged mutagenesis (stm) was used to identify genetic determinants of fitness associated with two key ecological processes mediated by bacteria. burkholderia vietnamiensis strain g4 was used as a model bacterium to investigate: phenol degradation as a model of bioremediation, and pea rhizosphere colonization as a prerequisite to biological control and phytoremediation. a total of 1900 mutants were screened and 196 putative fitness mutants identified; the genetic basis of 137 of these m ... | 2007 | 17359273 |
| effects of the inoculation of burkholderia vietnamensis and related endophytic diazotrophic bacteria on grain yield of rice. | during a survey of endophytic diazotrophic bacteria associated with different rice varieties in tamilnadu, some "endophytes" were obtained. thirteen bacterial isolates from surface-sterilized roots and shoots were obtained in pure culture, which produced indole acetic acid (iaa) and reduced acetylene to ethylene. polymerase chain reaction (pcr) amplification confirmed the presence of nif-h gene in all the isolates. morphological, biochemical, and molecular characteristics indicated that all of t ... | 2008 | 17406771 |
| reduction of nickel and uranium toxicity and enhanced trichloroethylene degradation to burkholderia vietnamiensis pr1301 with hydroxyapatite amendment. | the use of hydroxyapatite (ha) to sequester metals at mixed waste sites may reduce metal toxicity and facilitate microbial degradation of cocontaminant organics. the constitutive trichloroethylene (tce) degrader, burkholderia vietnamiensis pr1301, grew at 34.1 and 1.7 mm ni at ph 5 and 7, respectively, with 0.01 g ml(-1) ha compared to 17 and 0.85 mm ni without ha. pr1 grew at 4.2 mm u at ph 5 and 7 with 0.01 g ml(-1) ha compared to 1.1 mm u without ha. a similar decrease in the toxicity of ni a ... | 2007 | 17410778 |
| selection of nitrogen-fixing deficient burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations. | burkholderia vietnamiensis is the third most prevalent species of the burkholderia cepacia complex (bcc) found in cystic fibrosis (cf) patients. its ability at fixing nitrogen makes it one of the main bcc species showing strong filiations with environmental reservoirs. in this study, 83% (29 over 35) of the b. vietnamiensis cf isolates and 100% of the environmental ones (over 29) were found expressing the dinitrogenase complex (encoded by the nif cluster) which is essential in n(2) fixation. amo ... | 2007 | 17472633 |
| rapid multiplex immunofluorescent assay to detect antibodies against burkholderia pseudomallei and taxonomically closely related nonfermenters. | an indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (lps) of burkholderia pseudomallei and taxonomically closely related species was developed with the luminex system. lpss of pseudomonas aeruginosa, burkholderia cepacia, burkholderia thailandensis, burkholderia vietnamiensis, b. pseudomallei, and burkholderia mallei were successfully conjugated to luminex microspheres. antibodies measured against the lps of b. pseudomallei-conjugated luminex beads only cross- ... | 2007 | 17642542 |
| identification of genes conferring arsenic resistance to escherichia coli from an effluent treatment plant sludge metagenomic library. | the majority of bacteria elude culture in the laboratory. a metagenomic approach provides culture-independent access to the gene pool of the whole bacterial community. a metagenomic library was constructed from an industrial effluent treatment plant sludge containing about 1.25 gb of microbial community dna. two arsenic-resistant clones were selected from the metagenomic library. clones mt3 and mt6 had eight- and 18-fold higher resistance to sodium arsenate in comparison with the parent strain, ... | 2009 | 19016868 |
| changes in protein expression in burkholderia vietnamiensis pr1 301 at ph 5 and 7 with and without nickel. | burkholderia vietnamiensis pr1(301) (pr1) exhibits ph-dependent nickel (ni) tolerance, with lower ni toxicity observed at ph 5 than at ph 7. the ni tolerance mechanism in pr1 is currently unknown, and traditional mechanisms of ni resistance do not appear to be present. therefore, 2d gel electrophoresis was used to examine changes in protein expression in pr1 with and without ni (3.4 mm) at ph 5 and 7. proteins with both a statistically significant and at least a twofold difference in expression ... | 2008 | 19047749 |
| dna microarray-based detection and identification of burkholderia mallei, burkholderia pseudomallei and burkholderia spp. | we developed a rapid oligonucleotide microarray assay based on genetic markers for the accurate identification and differentiation of burkholderia (b.) mallei and burkholderia pseudomallei, the agents of glanders and melioidosis, respectively. these two agents were clearly identified using at least 4 independent genetic markers including 16s rrna gene, flic, motb and also by novel species-specific target genes, identified by in silico sequence analysis. specific hybridization signal profiles all ... | 2009 | 19366627 |
| isolation of burkholderia cenocepacia and burkholderia vietnamiensis from human sewage. | fresh human sewage was examined from a sewage treatment plant for the presence of members of the burkholderia cepacia complex (bcc) of bacterial organisms and confirmed the presence of viable b. cenocepacia and b. vietnamiensis, by a combination of cultural, phenotypic and genotypic techniques. both these organisms are important respiratory pathogens for patients with cystic fibrosis (cf). presently, the survival dynamics of these organisms in sewage effluent and sludge is, as yet, unknown. ther ... | 2009 | 19370465 |
| letter with regard to the publication of the manuscript entitled "effects of the inoculation of burkholderia vietnamiensis and related endophytic diazotrophic bacteria on grain yield of rice". | | 2009 | 19618099 |
| first structural characterization of burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains. | this is the first structural elucidation of the lipooligosaccharide (los) endotoxin isolated from burkholderia vietnamiensis, a clinically important member of burkholderia cepacia complex, a group of over 10 opportunistic species that are highly problematic in cystic fibrosis. we have characterized a novel los structure extracted from two clonal strains of b. vietnamiensis isolated from a cystic fibrosis patient who underwent lung transplantation. strains were selected from the pretransplantatio ... | 2009 | 19641092 |
| epidemiology and molecular characterization of a clone of burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a french intensive care unit. | clustered cases of nosocomial pulmonary infections were observed in a french intensive care unit. biochemical tests showed the etiologic agents to be part of the bcc (bcc). reca polymerase chain reaction-restriction fragment length polymorphism analysis and molecular phylogeny positioned the isolates into burkholderia cenocepacia. their reca sequences were found identical to those of et12 strains responsible of necrotic pneumonia in cystic fibrosis patients. analyses of a multi locus sequence ty ... | 2010 | 19716254 |
| burkholderia vietnamiensis isolated from root tissues of nipa palm (nypa fruticans) in sarawak, malaysia, proved to be its major endophytic nitrogen-fixing bacterium. | root-associating bacteria of the nipa palm (nypa fruticans), preferring brackish-water affected mud in sarawak, malaysia, were investigated. in a comparison of rhizobacterial microbiota between the nipa and the sago (metroxylon sagu) palm, it was found that the nipa palm possessed a group of burkholderia vietnamiensis as its main active nitrogen-fixing endophytic bacterium. acetylene reduction by the various isolates of b. vietnamiensis was constant (44 to 68 nmol h(-1) in ethylene production ra ... | 2010 | 20834139 |
| in vitro susceptibility of burkholderia vietnamiensis to aminoglycosides. | burkholderia cepacia complex (bcc) bacteria are opportunistic pathogens that can cause severe disease in cystic fibrosis (cf) patients and other immunocompromised individuals and are typically multidrug resistant. here we observed that unlike other bcc species, most environmental and clinical burkholderia vietnamiensis isolates were intrinsically susceptible to aminoglycosides but not to cationic antimicrobial peptides or polymyxin b. furthermore, strains acquired aminoglycoside resistance durin ... | 2011 | 21321142 |
| burkholderia cenocepacia creates an intramacrophage replication niche in zebrafish embryos, followed by bacterial dissemination and establishment of systemic infection. | bacteria belonging to the "burkholderia cepacia complex" (bcc) often cause fatal pulmonary infections in cystic fibrosis patients, yet little is know about the underlying molecular mechanisms. these gram-negative bacteria can adopt an intracellular lifestyle, although their ability to replicate intracellularly has been difficult to demonstrate. here we show that bcc bacteria survive and multiply in macrophages of zebrafish embryos. local dissemination by nonlytic release from infected cells was ... | 2010 | 20086083 |
| five novel acid-tolerant oligotrophic thiosulfate-metabolizing chemolithotrophic acid mine drainage strains affiliated with the genus burkholderia of betaproteobacteria and identification of two novel soxb gene homologues. | five acid-tolerant thiosulfate-metabolizing bacteria were isolated from acid mine drainage samples from garubathan, india. 16s rrna gene analysis revealed that the strains were affiliated with the genus burkholderia of the class of betaproteobacteria. comparative 16s rrna gene sequence analyses indicated that the strains designated as gah1 and gah2 produced a separate phylogenetic branch having burkholderia pyrrocinia atcc 51958(t) (96-98%) as the closest relative. strains gah4 and burkholderia ... | 2011 | 21349327 |
| the plant pathogen pseudomonas fuscovaginae contains two conserved quorum sensing systems involved in virulence and negatively regulated by rsal and the novel regulator rsam. | pseudomonas fuscovaginae is a gram-negative fluorescent pseudomonad pathogenic towards several plant species. despite its importance as a plant pathogen, no molecular studies of virulence have thus far been reported. in this study we show that p. fuscovaginae possesses two conserved n-acyl homoserine lactone (ahl) quorum sensing (qs) systems which we designated pfsi/r and pfvi/r. the pfsi/r system is homologous to the bvii/r system of burkholderia vietnamiensis and produces and responds to c10-h ... | 2011 | 20701623 |
| diversity analysis of burkholderia cepacia complex in the water bodies of west lake, hangzhou, china. | a survey of burkholderia cepacia complex (bcc) species was conducted in water bodies of west lake in china. a total of 670 bacterial isolates were recovered on selective media. out of them, 39.6% (265 isolates) were assigned to the following species: burkholderia multivorans, burkholderia cenocepacia reca lineage iiia, iiib, burkholderia stabilis, burkholderia vietnamiensis, and burkholderia seminalis while b. cenocepacia is documented as a dominant bcc species in water of west lake. in addition ... | 2011 | 21538256 |
| screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40. | the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated. | 2012 | 22017666 |
| Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry. | The main objectives of this work were to obtain a more extensive coverage of the Burkholderia vietnamiensis proteome than previously reported and to identify virulence factors using tandem mass spectrometry. The proteome of B. vietnamiensis was precipitated into four fractions to as extracellular, intracellular, cell surface and cell wall proteins. Two different approaches were used to analyze the proteins. The first was a gel-based method where 1D SDS-PAGE was used for separation of the protein ... | 2011 | 22187530 |
| Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113. | Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) inc ... | 2011 | 21972146 |
| revelation of the ability of burkholderia sp. usm (jcm 15050) pha synthase to polymerize 4-hydroxybutyrate monomer. | the nutrition-versatility of burkholderia sp. strain usm (jcm 15050) has initiated the studies on the use of this bacterium for polyhydroxyalkanoate (pha) production. to date, the burkholderia sp. has been reported to synthesize 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxy-4-methylvalerate monomers. in this study, the pha biosynthetic genes of this strain were successfully cloned and characterized. the pha biosynthetic cluster of this strain consisted of a pha synthase (phac), β-ketothiola ... | 2012 | 22877240 |
| hexavalent chromium reduction under fermentative conditions with lactate stimulated native microbial communities. | microbial reduction of toxic hexavalent chromium (cr(vi)) in-situ is a plausible bioremediation strategy in electron-acceptor limited environments. however, higher [cr(vi)] may impose stress on syntrophic communities and impact community structure and function. the study objectives were to understand the impacts of cr(vi) concentrations on community structure and on the cr(vi)-reduction potential of groundwater communities at hanford, wa. steady state continuous flow bioreactors were used to gro ... | 2013 | 24376771 |
| investigation of aminoglycoside resistance inducing conditions and a putative amrab-oprm efflux system in burkholderia vietnamiensis. | burkholderia cepacia complex (bcc) bacteria are highly virulent, typically multidrug-resistant, opportunistic pathogens in cystic fibrosis (cf) patients and other immunocompromised individuals. b. vietnamiensis is more often susceptible to aminoglycosides than other bcc species, and strains acquire aminoglycoside resistance during chronic cf infection and under tobramycin and azithromycin exposure in vitro, apparently from gain of antimicrobial efflux as determined through pump inhibition. the a ... | 2014 | 24393536 |
| activity of tobramycin against cystic fibrosis isolates of burkholderia cepacia complex grown as biofilms. | pulmonary infection with burkholderia cepacia complex in cystic fibrosis (cf) patients is associated with more-rapid lung function decline and earlier death than in cf patients without this infection. in this study, we used confocal microscopy to visualize the effects of various concentrations of tobramycin, achievable with systemic and aerosolized drug administration, on mature b. cepacia complex biofilms, both in the presence and absence of cf sputum. after 24 h of growth, biofilm thickness wa ... | 2015 | 26503664 |
| polyphasic characterisation of burkholderia cepaciacomplex species isolated from children with cystic fibrosis. | cystic fibrosis (cf) patients with burkholderia cepacia complex (bcc) pulmonary infections have high morbidity and mortality. the aim of this study was to compare different methods for identification of bcc species isolated from paediatric cf patients. oropharyngeal swabs from children with cf were used to obtain isolates of bcc samples to evaluate six different tests for strain identification. conventional (cpt) and automatised (apt) phenotypic tests, polymerase chain reaction (pcr)-reca, restr ... | 2016 | 26814642 |
| antibiotic resistance in burkholderia species. | the genus burkholderia comprises metabolically diverse and adaptable gram-negative bacteria, which thrive in often adversarial environments. a few members of the genus are prominent opportunistic pathogens. these include burkholderia mallei and burkholderia pseudomallei of the b. pseudomallei complex, which cause glanders and melioidosis, respectively. burkholderia cenocepacia, burkholderia multivorans, and burkholderia vietnamiensis belong to the burkholderia cepacia complex and affect mostly c ... | 2016 | 27620956 |
| bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases. | brine shrimp are aquatic crustaceans belonging to a genus of artemia. this organism is widely used for testing the toxicity of chemicals. in this study, brine shrimp were evaluated as an infection model organism to study bacterial virulence. artemia nauplii were infected with various pathogenic bacteria, such as vibrio vulnificus, pseudomonas aeruginosa, burkholderia vietnamiensis, staphylococcus aureus, and escherichia coli, and the susceptibility to these bacteria was investigated by counting ... | 2014 | 25420421 |
| accurate identification of members of the burkholderia cepacia complex in cystic fibrosis sputum. | the burkholderia cepacia complex (bcc) is a group of closely related species which includes opportunistic pathogens causing chronic respiratory infections in immunocompromised patients, or individuals affected by cystic fibrosis (cf). other burkholderia species causing infection in the cf population are burkholderia gladioli and burkholderia pseudomallei. traditional phenotypic analyses have been demonstrated to be inadequate for reliable identifications of isolates of bcc and b. gladioli. a pan ... | 2016 | 26671758 |
| arabidopsis thaliana and pisum sativum models demonstrate that root colonization is an intrinsic trait of burkholderia cepacia complex bacteria. | burkholderia cepacia complex (bcc) bacteria possess biotechnologically useful properties that contrast with their opportunistic pathogenicity. the rhizosphere fitness of bcc bacteria is central to their biocontrol and bioremediation activities. however, it is not known whether this differs between species or between environmental and clinical strains. we investigated the ability of 26 bcc strains representing nine different species to colonize the roots of arabidopsis thaliana and pisum sativum ... | 2014 | 24327425 |
| structure of a novel exopolysaccharide produced by burkholderia vietnamiensis, a cystic fibrosis opportunistic pathogen. | burkholderia vietnamiensis belongs to the burkholderia cepacia complex and is an opportunistic pathogen for cystic fibrosis patients. as many other burkholderia species, it has a mucoide phenotype, producing abundant exopolysaccharide. in general, polysaccharides contribute to bacterial survival in a hostile environment, are recognised as virulence factors and as important components in biofilm formation. the primary structure of the exopolysaccharide produced by b. vietnamiensis lmg 10929 was d ... | 2013 | 23544536 |
| sequential formation of two branched intermediates during protein splicing of class three inteins. | inteins are the protein equivalent of introns. they are seamlessly removed during post-translational maturation of their host protein (extein). inteins from extremophiles played a key role in understanding intein-mediated protein splicing. there are currently three classes of inteins defined by catalytic mechanism and sequence signatures. this study demonstrates splicing of three class 3 mini-inteins: burkholderia vietnamiensis g4 bvi icmo intein, mycobacterium smegmatis mc2 155 msm dnab-1 intei ... | 2017 | 27704298 |
| pathways of reductive degradation of crystal violet in wastewater using free-strain burkholderia vietnamiensis c09v. | a new strain isolated from activated sludge and identified as burkholderia vietnamiensis c09v was used to biodegrade crystal violet (cv) from aqueous solution. to understand the degradation pathways of cv, batch experiments showed that the degradation using b. vietnamiensis c09v significantly depended on conditions such as ph, initial dye concentration and media components, carbon and nitrogen sources. acceleration in the biodegradation of cv was observed in presence of metal ions such as cd and ... | 2014 | 24862483 |
| burkholderia vietnamiensis c09v as the functional biomaterial used to remove crystal violet and cu(ii). | burkholderia vietnamiensis c09v (b.v. c09v) was used to remove both crystal violet (cv) and cu(ii) because dye effluents often contain dyes and metal ions. inhibiting the strain׳s growth through the biosorption of cu(ii) on b.v. c09v and promoting its growth by using cv as a carbon source led to the degradation of cv (30mg/l). it fell to 36.9 percent and the amount of cu(ii) (50mg/l) removed rose to 34.9 percent in the presence of both cv and cu(ii). this outcome is comparable to the single pres ... | 2014 | 24780226 |
| enhancing co-metabolic degradation of trichloroethylene with toluene using burkholderia vietnamiensis g4 encapsulated in polyethylene glycol polymer. | the biodegradation potential of burkholderia vietnamiensis g4 (b. vietnamiensis g4) was evaluated under encapsulation in comparison with direct exposure to trichloroethylene (tce) (0.1, 0.5, 1 and 5 mg/l) and toluene (10 and 50 mg/l), maintaining aerobic conditions. b. vietnamiensis g4 was encapsulated in polyethylene glycol (peg) polymer. under suspended conditions, the degradation rate decreased as the initial tce concentration increased, even with a higher amount of substrate available. howev ... | 2015 | 24701945 |
| bacterial dynamics of biofilm development during toluene degradation by burkholderia vietnamiensis g4 in a gas phase membrane bioreactor. | in this study, the dynamics of living cells (lc) and dead cells (dc) in a laboratory-scale biofilm membrane bioreactor for waste gas treatment was examined. toluene was used as a model pollutant. the bacterial cells were enumerated as fluoromicroscopic counts during a 140 operating day period using baclight nucleic acid staining in combination with epifluorescence and confocal laser scanning microscopy (cslm). overall, five different phases could be distinguished during the biofilm development: ... | 2009 | 19809262 |
| complete nucleotide sequence of the self-transmissible tol plasmid pd2rt provides new insight into arrangement of toluene catabolic plasmids. | in the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pd2rt of pseudomonas migulae strain d2rt isolated from baltic sea water. the pd2rt is 129,894 base pairs in size with an average g+c content of 53.75%. a total of 135 open reading frames (orfs) were predicted to encode proteins, among them genes for catabolism of toluene, plasmid replication, maintenance and conjugative transfer. orfs encoding proteins with putative functions in stress response, tran ... | 2013 | 24095800 |
| origin, duplication and reshuffling of plasmid genes: insights from burkholderia vietnamiensis g4 genome. | using a computational pipeline based on similarity networks reconstruction we analysed the 1133 genes of the burkholderia vietnamiensis (bv) g4 five plasmids, showing that gene and operon duplication played an important role in shaping the plasmid architecture. several single/multiple duplications occurring at intra- and/or interplasmids level involving 253 paralogous genes (stand-alone, clustered or operons) were detected. an extensive gene/operon exchange between plasmids and chromosomes was a ... | 2014 | 24576463 |
| biodegradation of crystal violet using burkholderia vietnamiensis c09v immobilized on pva-sodium alginate-kaolin gel beads. | the strain, burkholderia vietnamiensis c09v was immobilized on pva-alginate-kaolin gel beads as a biomaterial to improve the degradation of crystal violet from aqueous solution. the results show that 98.6% (30 mg l(-1)) crystal violet was removed from aqueous solution using immobilized cells on pva-alginate-kaolin gel beads, while 94.0% crystal violet was removed by free cells after degradation at the ph 5 and 30°c for 30 h. kinetics studies show that the pseudo-second-order kinetics well descri ... | 2012 | 22789742 |
| removal of gaseous trichloroethylene (tce) in a composite membrane biofilm reactor. | a membrane biofilm reactor (mbfr) was investigated for the degradation of trichloroethylene (tce) vapors inoculated by burkholderia vietnamiensis g4. toluene (tol) was used as the primary substrate. the mbfr was loaded sequentially with tol, tce (or both) during 110 days. in this study, a maximum steady-state tce removal efficiency of 23% and a maximum volumetric elimination capacity (ec) of 2.1 g m(-3) h(-1) was achieved. a surface area based maximum elimination capacity (ec(m)) of 4.2 × 10(-3) ... | 2012 | 22486674 |