| expanding small-molecule functional metagenomics through parallel screening of broad-host-range cosmid environmental dna libraries in diverse proteobacteria. | the small-molecule biosynthetic diversity encoded within the genomes of uncultured bacteria is an attractive target for the discovery of natural products using functional metagenomics. phenotypes commonly associated with the production of small molecules, such as antibiosis, altered pigmentation, or altered colony morphology, are easily identified from screens of arrayed metagenomic library clones. however, functional metagenomic screening methods are limited by their intrinsic dependence on a h ... | 2010 | 20081001 |
| transgenic tomato plants alter quorum sensing in plant growth-promoting rhizobacteria. | two gram-negative, plant growth-promoting rhizobacteria (pgprs), denominated as m12 and m14, were classified by 16s rdna sequencing as burkholderia graminis species. both strains were shown to produce a variety of n-acyl-homoserine lactone (ahl) quorum sensing (qs) signalling molecules. the involvement of these molecules in plant growth promotion and the induction of protection against salt stress was examined. ahl production was evaluated in vitro by thin-layer chromatography using ahl biosenso ... | 2008 | 18384507 |
| classification of the biphenyl- and polychlorinated biphenyl-degrading strain lb400t and relatives as burkholderia xenovorans sp. nov. | strain lb400t is the best-studied polychlorinated biphenyl (pcb) degrader. this organism has previously been allocated in the genus burkholderia, since its 16s rrna gene sequence shows 98.6 % sequence similarity to the type strains of burkholderia graminis and burkholderia terricola. a polyphasic study was undertaken to clarify the actual taxonomic position of this biotechnologically important organism and of two strains, one recovered from a blood culture vial and one from a coffee plant rhizos ... | 2004 | 15388727 |
| burkholderia fungorum sp. nov. and burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. | a polyphasic taxonomic study that included dna-dna hybridizations, dna base ratio determinations, 16s rdna sequence analyses, whole-cell protein and fatty acid analyses and an extensive biochemical characterization was performed on 16 strains isolated from the environment, animals and human clinical samples. the isolates belonged to the genus burkholderia, were phylogenetically closely related to burkholderia graminis, burkholderia caribensis and burkholderia phenazinium and had g+c contents bet ... | 2001 | 11411678 |
| burkholderia graminis sp. nov., a rhizospheric burkholderia species, and reassessment of [pseudomonas] phenazinium, [pseudomonas] pyrrocinia and [pseudomonas] glathei as burkholderia. | in a survey of soil and wheat or maize rhizoplane bacteria isolated using a medium containing azelaic acid and tryptamine as sole carbon and nitrogen sources, respectively, a large proportion of burkholderia-like bacteria were found. among them, a homogeneous group of strains was identifiable based on phenotypic properties, fatty acid composition, dna-dna hybridizations and 16s rdna sequences. according to molecular data, this group belongs to the genus burkholderia but its weak similarity to pr ... | 1998 | 9731297 |