| biochemical, electrophoretic and immunohistochemical aspects of malate dehydrogenase in truffles (ascomycotina). | the malate dehydrogenase (mdh; ec 1.1.1.37; l-malate-nad(+)-oxidoreductase) activities of truffles of the genus tuber (tuber melanosporum vittad., tuber brumale vittad., tuber aestivum vittad., tuber magnatum pico, tuber rufum pico) have been characterized with regard to the k(m) and v(max) values in the direct and reverse reactions. the isoelectrofocusing has revealed bands showing pi values ranging from ph 5.85 to 7.8. the mdh of t. melanosporum has been partially purified by hydroxyapatite tr ... | 2000 | 10754250 |
| bacteria of the genus rhodopseudomonas (bradyrhizobiaceae): obligate symbionts in mycelial cultures of the black truffles tuber melanosporum and tuber brumale. | this work aimed at characterizing 12 isolates of the genus tuber including tuber melanosporum (11 isolates) and tuber brumale (one isolate). this was done using internal transcribed spacer (its) sequences, confirming their origin. | 2016 | 27468385 |
| mycorrhization of pecan (carya illinoinensis) with black truffles: tuber melanosporum and tuber brumale. | pecan, carya illinoinensis, is an economically important nut producing tree that can establish ectomycorrhizal symbiosis with a high diversity of fungi. in the southern usa, truffles (tuber spp.) sometimes fruit prolifically in cultivated pecan orchards and regularly associate with pecan roots as ectomycorrhizae (ecms). it has been demonstrated that some valuable european truffle species (e.g., tuber borchii and tuber aestivum) can form ecms with pecan seedlings in nursery conditions. thus, peca ... | 2017 | 27838857 |
| mycorrhiza analyses in new zealand truffières reveal frequent but variable persistence of tuber melanosporum in co-existence with other truffle species. | this study compiles the results from an examination of mycorrhizae on root samples from tuber melanosporum truffières in new zealand. samples were taken over 5 years from 328 trees in 43 truffières established with nursery-inoculated trees. mycorrhizae were analysed using a combination of morphological and molecular techniques, focusing on the identification of tuber species. results show that 49% of the trees, and nearly 90% of the truffières, retained t. melanosporum mycorrhizae up to 21 years ... | 2013 | 22752459 |
| truffle diversity (tuber, tuberaceae) in british columbia. | to improve baseline data for the developing truffle industry in british columbia, we compiled existing tuber species sequences from published and unpublished studies and generated new its sequences for truffles belonging to tuber collected in the province. in doing so, we obtained evidence that 13 species of tuber occur in the province, including six introduced and seven native species, two of which are putative undescribed species. of the native species, the tuber anniae species complex is wide ... | 2016 | 27083929 |
| phylogeny and phylogeography of the tuber brumale aggr. | the tuber brumale (winter truffle) is a black truffle reported from most european countries, belonging to the melanosporum group. its significance in the economy is ambivalent as the winter truffle has been shown to be a frequent contaminant species in the orchards of the perigord truffle and occasionally in those of the summer truffle, yet owing to its delicate fragrance, its trade is worthy of note. the phylogeny and phylogeography of economically important truffles are relatively well-explore ... | 2014 | 24604084 |
| comparison of ectomycorrhizal communities in natural and cultivated tuber melanosporum truffle grounds. | truffles are hypogeous ectomycorrhizal (em) fungi belonging to the genus tuber. although outplanting of truffle-inoculated host plants has enabled the realization of productive orchards, truffle cultivation is not yet standardized. therefore, monitoring the distribution of fungal species in different truffle fields may help us to elucidate the factors that shape microbial communities and influence the propagation and fruiting of tuber spp. in this study, we compared the fungal biodiversity in cu ... | 2012 | 22469019 |
| selection of a set of specific primers for the identification of tuber rufum: a truffle species with high genetic variability. | tuber rufum is a truffle widely distributed throughout europe, which forms mycorrhizal associations with numerous species of broadleaf and coniferous trees. the possibility of t. rufum contamination in commercial truffle-infected plants makes its detection important. to facilitate the identification of t. rufum from mycorrhiza and fruitbodies, species-specific primers were designed and tested. to overcome the high intraspecific genetic variability within the internal transcribed spacer (its) reg ... | 2007 | 18031344 |
| pattern of tuber melanosporum extramatrical mycelium expansion over a 20-year chronosequence in quercus ilex-truffle orchards. | successful cultivation of black truffle (tuber melanosporum) requires a long-term investment and the maintenance of the symbiosis throughout its preproductive and productive years. monitoring the symbiosis over time is challenging, as it requires methods that can detect the belowground proliferation of the fungus associated with its host tree. in this study, we used a chronosequence design to study the expansion pattern of this fungus as the host tree grows. we hypothesize that this expansion ca ... | 2014 | 24469788 |
| genetic diversity and mating type distribution of tuber melanosporum and their significance to truffle cultivation in artificially planted truffieres in australia. | tuber melanosporum is a truffle native to europe and is cultivated in countries such as australia for the gastronomic market, where production yields are often lower than expected. we assessed the genetic diversity of t. melanosporum with six microsatellite loci to assess the effect of genetic drift on truffle yield in australia. genetic diversity as assessed on 210 ascocarps revealed a higher allelic diversity compared to previous studies from europe, suggesting a possible genetic expansion and ... | 2012 | 22773652 |
| calcareous amendments to soils to eradicate tuber brumale from t. melanosporum cultivations: a multivariate statistical approach. | calcareous amendments are being used in tuber melanosporum truffle plantations in attempts to eradicate tuber brumale. however, there are no studies available which provide soil analysis and statistical data on this topic. we studied 77 soil samples to compare the values for carbonates, ph and total organic carbon in t. brumale truffières with the values for t. melanosporum truffières on contaminated farms and in natural areas. statistical analyses indicate that the concentrations of active carb ... | 2009 | 19156449 |
| comparative study and molecular characterization of ectomycorrhizas in tilia americana and quercus pubescens with tuber brumale. | mycorrhizas of tuber brumale on quercus pubescens and tilia americana were obtained in vitro using micropropagated plantlets. mycelium pure cultures were used for inoculation. both the mycelium used for the inoculations, as well as the mycorrhizas which were obtained, were identified using several molecular approaches: analysis of the its region, polymerase chain reaction (pcr) specific primers and sequencing. the mycorrhizas were described from a morphological standpoint. some of their biometri ... | 2002 | 12423745 |
| tilia platyphyllos scop.-tuber brumale vittad. vs. t. platyphyllos scop.-t. borchii vittad. ectomycorrhizal systems: a comparison of structural and functional traits. | ectomycorrhizae are mutualistic associations of several species of fungi with higher plants. their formation involves alterations in the morphology and cell structure of the plant root and fungal mycelium. these modifications are correlated with mrna and protein synthesis in the two symbionts. to gain more information about structural and functional traits involved in ectomycorrhizal formation, two "in vitro" ectomycorrhizal systems, set up by the inoculation of tilia platyphyllos scop. roots wi ... | 2005 | 16122938 |
| multi-cropping edible truffles and sweet chestnuts: production of high-quality castanea sativa seedlings inoculated with tuber aestivum, its ecotype t. uncinatum, t. brumale, and t. macrosporum. | the plantation and management of sweet chestnut (castanea sativa mill.) orchards is a common and traditional land use system in many areas of europe that offers the advantage of simultaneous production of nuts and timber. during the last decades, sweet chestnut has declined dramatically in many regions because of the profound social changes in rural areas coupled with pathogen attacks. truffles, the hypogeous ascocarps of the ectomycorrhizal genus tuber, are currently cultivated using host trees ... | 2018 | 29101543 |