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queensland fruit fly virus, a probable member of the picornaviridae.a picornavirus was isolated from various life stages of the queensland fruit fly, dacus tryoni. this virus, queensland fruit fly virus (qffv) has virions with a diameter of 30 nm and a sedimentation coefficient of 178 s. one third of the particles in preparations were empty capsids or natural top component (ntc) with a sedimentation coefficient of 95 s. the buoyant density (rho) of virions and ntc in cscl was 1.34 and 1.30 g/ml respectively; small amounts of a dense component (rho = 1.45 g/ml) w ...19883133998
the white gene of the tephritid fruit fly bactrocera tryoni is characterized by a long untranslated 5' leader and a 12kb first intron.a 300 bp fragment from exon 6 of the white gene of bactrocera tryoni was used to screen a b. tryoni genomic library. one positive (approximately 14 kb) insert contained exons 2-6 of white by nucleotide and amino acid sequence similarity to the white genes of d. melanogaster (o'hare et al., 1984; pepling & mount, 1990). lucilia cuprina (garcia et al., 1996). ceratitis capitata (zwiebel et al., 1995) and anopheles gambiae (besansky et al., 1995). a white 5' cdna fragment containing exons 1, 2 and ...19979359576
Additive and interactive effects of nutrient classes on longevity, reproduction, and diet consumption in the Queensland fruit fly (Bactrocera tryoni).Insect lifespan is often closely linked to diet, and diet manipulations have been central to studies of ageing. Recent research has found that lifespan for some flies is maximised on a very low yeast diet, but once all yeast is removed, lifespan drops precipitously. Although effects of yeast availability on lifespan are commonly interpreted in terms of protein, yeast is a complex mix of nutrients and provides a rich source of vitamins, minerals and sterols. Elucidating which components of yeast ...201122094291
interspecific hybridization as a source of novel genetic markers for the sterile insect technique in bactrocera tryoni (diptera: tephritidae).bactrocera tryoni (froggatt) (diptera: tephritidae) or "qfly," is the most serious horticultural pest in australia, with a bioclimatic range that extends from the tropical north to the temperate south. various australian horticultural exports depend on certification that they originated from b. tryoni-free areas. to eliminate, rather than suppress, b. tryoni in production areas, a sterile insect technique (sit) campaign directed at b. tryoni has been in operation in southeastern australia since ...201020857713
pest fruit fly (diptera: tephritidae) in northwestern australia: one species or two?since 1985, a new and serious fruit fly pest has been reported in northwestern australia. it has been unclear whether this pest was the supposedly benign endemic species, bactrocera aquilonis, or a recent introduction of the morphologically near-identical queensland fruit fly, b. tryoni. b. tryoni is a major pest throughout eastern australia but is isolated from the northwest region by an arid zone. in the present study, we sought to clarify the species status of these new pests using an extensi ...201019602297
laboratory adaptation of bactrocera tryoni (diptera: tephritidae) decreases mating age and increases protein consumption and number of eggs produced per milligram of protein.a significant reduction in age of mating occurred during the first four generations (g1-g4) of laboratory adaptation of wild bactrocera tryoni (froggatt) and this was associated with the earlier attainment of peak egg load although no significant differences were detected in the peak egg load itself. a long term laboratory (ltl) strain had a significantly earlier mating age and higher peak egg load than flies of wild origin or those from the first four laboratory generations. the amount of prote ...200415541191
inferring modes of colonization for pest species using heterozygosity comparisons and a shared-allele test.long-range dispersal of a species may involve either a single long-distance movement from a core population or spreading via unobserved intermediate populations. where the new populations originate as small propagules, genetic drift may be extreme and gene frequency or assignment methods may not prove useful in determining the relation between the core population and outbreak samples. we describe computationally simple resampling methods for use in this situation to distinguish between the diffe ...200312618417
microsatellite analysis of the queensland fruit fly bactrocera tryoni (diptera: tephritidae) indicates spatial structuring: implications for population control.the population structure of a tephritid pest species, the queensland fruit fly bactrocera tryoni (froggatt), has been analysed over a five year period (1994-1998), using six microsatellites. adult fly samples were collected to cover most regions of eastern and central australia where the flies are regularly found. tests for heterogeneity indicated that flies within geographically defined regions were homogeneous. the samples were allocated into five regions, including one very large region, quee ...200111260729
interplasmid transposition of drosophila hobo elements in non-drosophilid insects.a modified hobo element from drosophila melanogaster was introduced into embryos of the housefly, musca domestica (family muscidae) and the queensland fruitfly, bactrocera tryoni (family tephritidae) to assess its ability to transpose. hobo was capable of transposition in these species and transposition products had all of the hallmarks of hobo transposition products recovered from d. melanogaster, including the movement only of sequences precisely delimited by the inverted terminal repeats of h ...19948041366
the transposable element mariner can excise in non-drosophilid insects.plasmid-based excision assays performed in embryos of two non-drosophilid species using the mariner transposable element from drosophila mauritiana resulted in empty excision sites identical to those observed after the excision of mariner from d. mauritiana chromosomes. in the presence of the autonomous mariner element mos1, excision products were recovered from d. melanogaster, d. mauritiana and the blowfly lucilia cuprina. when a hsp82 heat shock promoter-mos1 construct was used to supply mari ...19957500947
host susceptibility of citrus cultivars to queensland fruit fly (diptera: tephritidae).citrus crops are considered to be relatively poor hosts for queensland fruit fly, bactrocera tryoni (froggatt), as for other tephritid species. australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. in this study we conducted laboratory tests and field surveys to determine susceptibility to b. tryoni of six citrus cultivars [(eureka lemon (citrus limon (l.) osbeck); navel and valenci ...201323786078
relative tolerance of six bactrocera (diptera: tephritidae) species to phytosanitary cold treatment.to compare relative cold treatment tolerance across the economically important tephritid fruit flies (diptera: tephritidae), bactrocera carambolae drew & hancock, bactrocera correcta (bezzi), bactrocera cucurbitae (coquillett), four populations of bactrocera dorsalis (hendel), bactrocera zonata (saunders), and bactrocera tryoni (froggatt), eggs (in vitro), and larvae (in infested fruit or on carrot diet) were cold treated at 2.0 ± 0.2 °c for selected durations. the study was performed to assess ...201627660425
isolation and characterization of the bactrocera oleae genes orthologous to the sex determining sex-lethal and doublesex genes of drosophila melanogaster.here we report the isolation and characterization of the olive fruit fly bactrocera oleae genes orthologous to the drosophila melanogaster sex-determining genes sex-lethal (sxl) and doublesex (dsx). fragments of the sxl and dsx orthologous were isolated with rt-pcr. genomic and cdna clones were then obtained by screening a genomic library and separate male and female cdna adult libraries using the rt-pcr products as probes in both cases. b. oleae sxl gene (bosxl) expresses the same pattern of tr ...200515777677
ultrastructure of male reproductive accessory glands and ejaculatory duct in the queensland fruit fly, bactrocera tryoni (diptera: tephritidae).ultrastructure of male reproductive accessory glands and ejaculatory duct in the queensland fruit fly (q-fly), bactrocera tryoni, were investigated and compared with those of other tephritid flies. male accessory glands were found to comprise one pair of mesodermic glands and three pairs of ectodermic glands. the mesodermic accessory glands consist of muscle-lined, binucleate epithelial cells, which are highly microvillated and extrude electron-dense secretions by means of macroapocrine transpor ...200919026762
the complete nucleotide sequence of the mitochondrial genome of bactrocera minax (diptera: tephritidae).the complete 16,043 bp mitochondrial genome (mitogenome) of bactrocera minax (diptera: tephritidae) has been sequenced. the genome encodes 37 genes usually found in insect mitogenomes. the mitogenome information for b. minax was compared to the homologous sequences of bactrocera oleae, bactrocera tryoni, bactrocera philippinensis, bactrocera carambolae, bactrocera papayae, bactrocera dorsalis, bactrocera correcta, bactrocera cucurbitae and ceratitis capitata. the analysis indicated the structure ...201424964138
characterization of irritans mariner-like elements in the olive fruit fly bactrocera oleae (diptera: tephritidae): evolutionary implications.genomic variation among species is commonly driven by transposable element (te) invasion; thus, the pattern of tes in a genome allows drawing an evolutionary history of the studied species. this paper reports in vitro and in silico detection and characterization of irritans mariner-like elements (mles) in the genome and transcriptome of bactrocera oleae (rossi) (diptera: tephritidae). eleven irritans mle sequences have been isolated in vitro using terminal inverted repeats (tirs) as primers, and ...201627392643
raspberry ketone supplement promotes early sexual maturation in male queensland fruit fly, bactrocera tryoni (diptera: tephritidae).raspberry ketone (rk) is highly attractive to sexually mature, but not immature, males of many bactrocera species, including queensland fruit fly ('qfly', bactrocera tryoni), and acts as a metabolic enhancer in a wide diversity of animals. we considered the possibility that, as a metabolic enhancer, rk in adult diet might accelerate sexual maturation of male qflies.201728139095
protein:carbohydrate ratios explain life span patterns found in queensland fruit fly on diets varying in yeast:sugar ratios.dietary restriction extends life span across a vast diversity of taxa, but significant challenges remain in elucidating the underlying mechanisms. distinguishing between caloric and nutrient effects is an essential step. recent studies with drosophila and tephritid fruit flies have reported increased life span as dietary yeast-to-sugar ratios decreased and these effects have been attributed to changes in protein-to-carbohydrate (p:c) ratios of the diets rather than calories. however, yeast is a ...201121904823
alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni.bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ...201020883132
nutrients, not caloric restriction, extend lifespan in queensland fruit flies (bactrocera tryoni).caloric restriction (cr) has been widely accepted as a mechanism explaining increased lifespan (ls) in organisms subjected to dietary restriction (dr), but recent studies investigating the role of nutrients have challenged the role of cr in extending longevity. fuelling this debate is the difficulty in experimentally disentangling cr and nutrient effects due to compensatory feeding (cf) behaviour. we quantified cf by measuring the volume of solution imbibed and determined how calories and nutrie ...200919558564
kinetics of colonization of adult queensland fruit flies (bactrocera tryoni) by dinitrogen-fixing alimentary tract bacteria.the average total population of bacteria remained constant in the alimentary tracts of adult laboratory-raised queensland fruit flies (bactrocera tryoni) although the insects had ingested large numbers of live bacteria as part of their diet. the mean number of bacteria (about 13 million) present in the gut of the insects from 12 to 55 days after emergence was not significantly modified when, at 5 days after emergence, the flies were fed antibiotic-resistant bacteria belonging to two species comm ...199416349328
specialized pheromone and lure application technology as an alternative male annihilation technique to manage bactrocera tryoni (diptera: tephritidae).the results of this study suggest that a novel male annihilation technique (specialized pheromone and lure application technology [splat] incorporating cue-lure [cl] plus spinosad) is as effective as industry standard male annihilation controls, and is worth exploring further to managebactrocera tryoni(froggatt) populations. three lures were evaluated in a contact and feeding bioassay and a cage attractancy trial: 1) splat-cl + spinosad; 2) splat-cl without spinosad; and 3) wick-cl + malathion. ...201627018434
the mating system of the true fruit fly bactrocera tryoni and its sister-species, bactrocera neohumeralis.the frugivorous 'true' fruit fly, bactrocera tryoni (queensland fruit fly), is presumed to have a non-resourced-based lek mating system. this is largely untested, and contrary data exists to suggest bactrocera tryoni may have a resource-based mating system focused on fruiting host plants. we tested the mating system of bactrocera tryoni, and its close sibling bactrocera neohumeralis, in large field cages using laboratory reared flies. we used observational experiments that allowed us to determin ...201627006172
interplasmid transposition of the mariner transposable element in non-drosophilid insects.plasmid-based transposition assays were performed in developing embryos of the australian sheep blowfly lucilia cuprina and the queensland fruit fly bactrocera tryoni, using the mariner transposable element from drosophila mauritiana. transposition products were recovered that were identical in structure to those recovered from d. melanogaster. only sequences delimited by the mariner terminal repeats were transposed and all insertions occurred at ta residues, and duplicated these. these are the ...19979079884
genetic delineation of sibling species of the pest fruit fly bactocera (diptera: tephritidae) using microsatellites.using a large set of microsatellites, the genetic relationships between three closely related australian fruit fly species, bactrocera tryoni (froggatt), b. neohumeralis (hardy) and b. aquilonis(may) were investigated. bactrocera tryoni and b. neohumeralis are sympatric, while b. aquilonisis allopatric to both. the sympatric species, b. tryoni and b. neohumeralis, were found to be genetically distinct. it is likely that despite differences in mating time between these two species, some gene flow ...200312908921
field evaluation of melolure, a formate analogue of cuelure, and reassessment of fruit fly species trapped in sydney, new south wales, australia.in australia, tephritids are usually attracted to either cuelure or methyl eugenol. methyl eugenol is a very effective lure, but cuelure is less effective likely due to low volatility. a new formate analogue of cuelure, melolure, has increased volatility, resulting in improved efficacy with the melon fruit fly, bactrocera cucurbitae coquillett. we tested the efficacy of melolure with fruit fly species in sydney as part of the national exotic fruit fly monitoring programme. this monitoring progra ...201526470243
longevity of mass-produced bactrocera tryoni (diptera: tephritidae) held without food or water.the sterile insect technique is used to manage or control fruit flies throughout the world. the technique relies on large scale production before delivery to release managers. as part of the mass production phase, there are many quality control tests to demonstrate and maintain high quality pupae and flies. one highly desirable characteristic is adults with a long life so that these adults can reach sexual maturity and sterile males mate with wild fertile flies in the field and thus produce no v ...201426470075
wolbachia pseudogenes and low prevalence infections in tropical but not temperate australian tephritid fruit flies: manifestations of lateral gene transfer and endosymbiont spillover?maternally inherited wolbachia bacteria infect many insect species. they can also be transferred horizontally into uninfected host lineages. a wolbachia spillover from an infected source population must occur prior to the establishment of heritable infections, but this spillover may be transient. in a previous study of tephritid fruit fly species of tropical australia we detected a high incidence of identical wolbachia strains in several species as well as wolbachia pseudogenes. here, we have in ...201526385192
the microbiome of field-caught and laboratory-adapted australian tephritid fruit fly species with different host plant use and specialisation.tephritid fruit fly species display a diversity of host plant specialisation on a scale from monophagy to polyphagy. furthermore, while some species prefer ripening fruit, a few are restricted to damaged or rotting fruit. such a diversity of host plant use may be reflected in the microbial symbiont diversity of tephritids and their grade of dependency on their microbiomes. here, we investigated the microbiome of six tephritid species from three genera, including species that are polyphagous pest ...201525666536
the draft genome of the pest tephritid fruit fly bactrocera tryoni: resources for the genomic analysis of hybridising species.the tephritid fruit flies include a number of economically important pests of horticulture, with a large accumulated body of research on their biology and control. amongst the tephritidae, the genus bactrocera, containing over 400 species, presents various species groups of potential utility for genetic studies of speciation, behaviour or pest control. in australia, there exists a triad of closely-related, sympatric bactrocera species which do not mate in the wild but which, despite distinct mor ...201425527032
expression patterns of sex-determination genes in single male and female embryos of two bactrocera fruit fly species during early development.in tephritids, the sex-determination pathway follows the sex-specific splicing of transformer (tra) mrna, and the cooperation of tra and transformer-2 (tra-2) to effect the sex-specific splicing of doublesex (dsx), the genetic double-switch responsible for male or female somatic development. the dominant male determiner (m) is the primary signal that controls this pathway. m, as yet uncharacterized, is y-chromosome linked, expressed in the zygote and directly or indirectly diminishes active tra ...201425116961
combined effects of dietary yeast supplementation and methoprene treatment on sexual maturation of queensland fruit fly.yeast hydrolysate supplements promote maturation of many tephritid flies targeted for control using the sterile insect technique (sit), including queensland fruit fly (bactrocera tryoni; 'q-fly'). recently, application of the juvenile hormone analogue methoprene has been demonstrated to further promote maturation in some species. we here investigate the separate and combined effects of yeast hydrolysate and methoprene treatment on sexual maturation of sterile male and female q-flies. two methods ...201424424344
an improved culturing method for opiine fruit fly parasitoids and its application to parasitoid monitoring in the field.good culturing methods play an important role in the study of insect behavior and its application to pest management. here, we describe and validate a new method for rearing the parasitoid wasp, diachasmimorpha kraussii, which attacks some of the world's worst fruit fly pests and is an internationally used biological control agent. our method differs from standard culturing approaches by presenting adult wasps with host-infested artificial media within a "culturing bag," which mimics a natural ( ...201627650570
yeast: an overlooked component of bactrocera tryoni (diptera: tephritidae) larval gut microbiota.yeasts, often in hydrolyzed form, are key ingredients in the larval and adult diets of tephritid fruit fly colonies. however, very little is known about the presence or role of yeasts in the diets of tephritid fruit flies in nature. previous studies have identified bacteria but not detected yeasts in the gut of queensland fruit fly, bactrocera tryoni (froggatt), one of australia's most economically damaging insect pests of horticultural crops and of significant biosecurity concern domestically a ...201728039426
evaluating irradiation dose for sterility induction and quality control of mass-produced fruit fly bactrocera tryoni (diptera: tephritidae).the sterile insect technique has been routinely used to eradicate fruit fly bactrocera tryoni (froggatt) incursions. this study considers whether fly quality in a mass-rearing facility can be improved by reducing irradiation doses, without sacrificing reproductive sterility. pupae were exposed to one of five target irradiation dose ranges: 0, 40-45, 50-55, 60-65, and 70-75 gy. pupae were then assessed using routine quality control measures: flight ability, sex ratio, longevity under nutritional ...201425026679
estimating effective population size from linkage disequilibrium between unlinked loci: theory and application to fruit fly outbreak populations.there is a substantial literature on the use of linkage disequilibrium (ld) to estimate effective population size using unlinked loci. the ne estimates are extremely sensitive to the sampling process, and there is currently no theory to cope with the possible biases. we derive formulae for the analysis of idealised populations mating at random with multi-allelic (microsatellite) loci. the 'burrows composite index' is introduced in a novel way with a 'composite haplotype table'. we show that in a ...201323894410
do fruit ripening volatiles enable resource specialism in polyphagous fruit flies?frugivorous tephritid fruit flies have lineages with high levels of host generalism. these insects use olfaction to locate fruits, but how they are able to recognize the odors of so many different host species is poorly understood. we used a series of behavioral experiments to investigate the role of fruit ripening volatiles as host cues in the queensland fruit fly, bactrocera tryoni (froggatt), a polyphagous pest in australia. odors of mature guava (psidium guajava) attracted female and male fl ...201627586434
exposure of insect cells to ionising radiation in vivo induces persistent phosphorylation of a h2ax homologue (h2avb).the response of eukaryotic cells to ionising radiation (ir)-induced double-strand dna breaks is highly conserved and involves a dna repair mechanism characterised by the early phosphorylation of histone protein h2ax (producing the active form γh2ax). although the expression of an induced γh2ax variant has been detected in drosophila melanogaster, the expression and radiation response of a γh2ax homologue has not been reported in economically important fruit flies. we use bactrocera tryoni (dipte ...201323793612
diet quality mediates activity patterns in adult queensland fruit fly (bactrocera tryoni).studies linking resource acquisition and trait expression have traditionally treated nutritional resources as a single currency, but recent research has shown that trait expression can depend as much on diet quality (nutrient composition) as on diet quantity (calories). here, we investigate the role of nutrient composition and diet concentration on activity levels of adult queensland fruit flies (bactrocera tryoni froggatt: tephritidae). male and female flies were fed diets that varied in the pr ...201323623835
raspberry ketone analogs: vapour pressure measurements and attractiveness to queensland fruit fly, bactrocera tryoni (froggatt) (diptera: tephritidae).the queensland fruit fly, bactrocera tryoni (froggatt) (q-fly), is a major horticultural pest in eastern australia. effective monitoring, male annihilation technique (mat) and mass trapping (mt) are all important for control and require strong lures to attract flies to traps or toxicants. lure strength is thought to be related in part to volatility, but little vapour pressure data are available for most q-fly lures. raspberry ketone (4-(4-hydroxyphenyl)-2-butanone) and analogs that had esters (a ...201627196605
cost of reproduction in the queensland fruit fly: y-model versus lethal protein hypothesis.the trade-off between lifespan and reproduction is commonly explained by differential allocation of limited resources. recent research has shown that the ratio of protein to carbohydrate (p : c) of a fly's diet mediates the lifespan-reproduction trade-off, with higher p : c diets increasing egg production but decreasing lifespan. to test whether this p : c effect is because of changing allocation strategies (y-model hypothesis) or detrimental effects of protein ingestion on lifespan (lethal prot ...201223097519
helitrons shaping the genomic architecture of drosophila: enrichment of dine-tr1 in α- and β-heterochromatin, satellite dna emergence, and pirna expression.drosophila interspersed elements (dines) constitute an abundant but poorly understood group of helitrons present in several drosophila species. the general structure of dines includes two conserved blocks that may or not contain a region with tandem repeats in between. these central tandem repeats (ctrs) are similar within species but highly divergent between species. it has been assumed that ctrs have independent origins. herein, we identify a subset of dines, termed dine-tr1, which contain hom ...201526408292
a generic individual-based spatially explicit model as a novel tool for investigating insect-plant interactions: a case study of the behavioural ecology of frugivorous tephritidae.computational modelling of mechanisms underlying processes in the real world can be of great value in understanding complex biological behaviours. uptake in general biology and ecology has been rapid. however, it often requires specific data sets that are overly costly in time and resources to collect. the aim of the current study was to test whether a generic behavioural ecology model constructed using published data could give realistic outputs for individual species. an individual-based model ...201626999285
raspberry ketone trifluoroacetate, a new attractant for the queensland fruit fly, bactrocera tryoni (froggatt).queensland fruit fly, bactrocera tryoni (q-fly), is a major pest of horticultural crops in eastern australia. lures that attract male q-fly are important for detection of incursions and outbreaks, monitoring of populations, and control by mass trapping and male annihilation. cuelure, an analog of naturally occurring raspberry ketone, is the standard q-fly lure, but it has limited efficacy compared with lures that are available for some other fruit flies such as methyl eugenol for b. dorsalis. me ...201626922349
expression of the rubber-like protein, resilin, in developing and functional insect cuticle determined using a drosophila anti-rec 1 resilin antibody.the natural elastomeric protein, insect resilin, is the most efficient elastic material known, used to store energy for jumping and flight in a variety of insects. here, an antibody to recombinant drosophila melanogaster pro-resilin is used to examine resilin expression in drosophila and a wider range of insects.201222275226
parasitoids of queensland fruit fly bactrocera tryoni in australia and prospects for improved biological control.this review draws together available information on the biology, methods for study, and culturing of hymenopteran parasitoids of the queensland fruit fly, bactrocera tryoni, and assesses prospects for improving biological control of this serious pest. augmentative release of the native and naturalised australian parasitoids, especially the braconid diachasmimorpha tryoni, may result in better management of b. tryoni in some parts of australia. mass releases are an especially attractive option fo ...201226466726
biosynthesis of the spiroacetal suite in bactrocera tryoni.in pursuit of a more environmentally benign method of controlling the highly pestiferous queensland fruit fly, bactrocera tryoni, the biosynthesis of the minor components in the suite of spiroacetals released by females has been investigated. this follows on the biosynthetic definition of the pathway to the major component, (e,e)-1. the origins of the c(12) and c(13) spiroacetals (e,e)-2 and (e,e)-3, respectively, have been investigated by the administration of over 30 deuterated potential precu ...201121154610
germ-line transformation of the queensland fruit fly, bactrocera tryoni, using a piggybac vector in the presence of endogenous piggybac elements.we report the heritable germ-line transformation of the queensland fruit fly, bactrocera tryoni, using a piggybac vector marked with either the fluorescent protein dsred or egfp. a transformation frequency of 5-10% was obtained. inheritance of the transgenes has remained stable over more than 15 generations despite the presence of endogenous piggybac sequences in the b. tryoni genome. the sequence of insertion sites shows the usual canonical pattern of piggybac integraton into ttaa target sites. ...201120859652
flight control of fruit flies: dynamic response to optic-flow and headwind.insects are magnificent fliers that are capable of performing many complex tasks such as speed regulation, smooth landings, and collision avoidance, even though their computational abilities are limited by their small brain. to investigate how flying insects respond to changes in wind speed and surrounding optic flow, the open-loop sensorimotor response of female queensland fruit flies (bactrocera tryoni) was examined. 136 flies were exposed to stimuli comprising sinusoidally varying optic flow ...201728314748
yeast hydrolysate supplementation increases field abundance and persistence of sexually mature sterile queensland fruit fly, bactrocera tryoni (froggatt).the sterile insect technique (sit) is a non-chemical approach used to control major pests from several insect families, including tephritidae, and entails the mass-release of sterile insects that reduce fertility of wild populations. for sit to succeed, released sterile males must mature and compete with wild males to mate with wild females. to reach sexual maturity, the queensland fruit fly, bactrocera tryoni (froggatt) (diptera: tephritidae), must obtain adequate nutrition after adult emergenc ...201424456807
field performance of lynfield and mcphail traps for monitoring male and female sterile bactrocera tryoni (froggatt) and wild dacus newmani (perkins).mcphail traps, baited with protein food lure, are used worldwide for surveillance of many species of fruit flies. queensland fruit fly (qfly) bactrocera tryoni (froggatt) (diptera: tephritidae) is a native australian fruit fly and normally monitored using lynfield traps baited with cuelure. on some occasions, mcphail traps with wet food lures are deployed to detect female flies or to find the incursion epicentre. this paper reviews field results on the merits of lynfield and mcphail traps for de ...201020309994
the genetic structure of populations of an invading pest fruit fly, bactrocera tryoni, at the species climatic range limit.previous population genetic studies of the queensland fruit fly, bactrocera tryoni froggatt (diptera: tephritidae), in its central range have shown barely detectable genetic differentiation across distances of almost 3000 km (f(st)=0.003). in this study, we investigated the genetic structuring of southern border populations of b. tryoni, in the region extending from the central population to the recently colonized southern range limit. the expectation was that marginal populations would be small ...201019997126
impact of introduction of bactrocera dorsalis (diptera: tephritidae) and classical biological control releases of fopius arisanus (hymenoptera: braconidae) on economically important fruit flies in french polynesia.oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), was discovered on tahiti island in july 1996. eradication programs were conducted from 1997 to 2001, but failed. from 1998 to 2006, b. dorsalis was recovered from 29 different host fruit from the five society islands: tahiti, moorea, raiatea, tahaa, and huahine. analysis of coinfestation patterns by b. dorsalis, bactrocera tryoni (froggatt), and bactrocera kirki (froggatt) suggested b. dorsalis had displaced these two speci ...200717598524
synthesis and absolute configuration of a constitutionally-new [5.6] spiroacetal from b. tryoni (queensland fruit fly).a novel spiroacetal, (2s,6r,8s)-2-methyl-8-ethyl-1,7-dioxaspiro[5.6]dodecane (1), has been identified from the volatile secretions of female b. tryoni by mass spectral analysis and synthesis of an authentic, enantioenriched sample.200717377665
a diverse suite of spiroacetals, including a novel branched representative, is released by female bactrocera tryoni (queensland fruit fly).a remarkably diverse suite of spiroacetals including a novel member of the rare, branched chain class has been identified in the glandular secretions of bactrocera tryoni, the most destructive horticultural pest in australia.200617003870
control of copula duration and sperm storage by female queensland fruit flies.copula duration and sperm storage patterns can directly or indirectly affect fitness of male and female insects. although both sexes have an interest in the outcome, research has tended to focus on males. to investigate female influences, we compared copula duration and sperm storage of queensland fruit fly females that were intact, or had been incapacitated through decapitation or abdomen isolation. we found that copulations were far longer when females had been incapacitated, indicating that c ...201020650280
effect of adult chill treatments on recovery, longevity and flight ability of queensland fruit fly, bactrocera tryoni (froggatt) (diptera: tephritidae).control of queensland fruit fly, bactrocera tryoni (froggatt) (diptera: tephritidae), populations or outbreaks may be achieved through the mass-rearing and inundative release of sterile b. tryoni. an alternative release method is to release chilled adult sterile fruit flies to decrease packaging and transport requirements and potentially improve release efficiencies. two trials were conducted to determine the effect of chilling on the performance of two separate batches of adult b. tryoni, fed e ...201120609276
a role for copula duration in fertility of queensland fruit fly females mated by irradiated and unirradiated males.females of many tephritid fruit flies can mate more than once, and can store ejaculates from multiple males. as well as being an important element of reproductive biology, multiple mating by females is of particular relevance for sterile insect technique programs used to control major tephritid pests. here we investigate the consequences of multiple mating on fertility of queensland fruit fly (bactrocera tryoni) females sequentially mated to irradiated ('sterile') and unirradiated ('normal') mal ...201222906778
the effects of selection for early (day) and late (dusk) mating lines of hybrids of bactrocera tryoni and bactrocera neohumeralis.bactrocera neohumeralis and bactrocera tryoni are closely related tephritid fruit fly species. b. neohumeralis mates throughout the day (in bright light) and b. tryoni mates at dusk. the two species can also be distinguished by the colour of their calli (prothoracic sclerites) which are brown and yellow, respectively. the f1 hybrids can mate both in bright light just before dusk and during dusk and have calli that are partly brown and partly yellow. the f2 hybrids have a wider range of callus pa ...200314686607
genetic consequences of domestication and mass rearing of pest fruit fly bactrocera tryoni (diptera: tephritidae).tephritid fruit flies, an important pest of horticulture worldwide, are increasingly targeted for control or eradication by large-scale releases of sterile flies of the same species. for each species treated, strains must be domesticated for mass rearing to provide sufficiently large numbers of individuals for releases. increases in productivity of domesticated tephritid strains are well documented, but there have been few systematic studies of the genetic consequences of domestication in tephri ...201222812147
barcoding queensland fruit flies (bactrocera tryoni): impediments and improvements.identification of adult fruit flies primarily involves microscopic examination of diagnostic morphological characters, while immature stages, such as larvae, can be more problematic. one of the australia's most serious horticultural pests, the queensland fruit fly (bactrocera tryoni: tephritidae), is of particular biosecurity/quarantine concern as the immature life stages occur within food produce and can be difficult to identify using morphological characteristics. dna barcoding of the mitochon ...201222369549
the integument of the queensland fruit fly, dacus tryoni (frogg.). i. the tergal glands. 19675601899
development and ultrastructure of the fat body cells and ocnocytes of the queensland fruit fly, dacus tryoni (frogg.). 19675601902
the integument of the queensland fruit fly, dacus tryoni (frogg.). ii. development and ultrastructure of the abdominal integument and bristles. 19675601901
carbohydrate diet and reproductive performance of a fruit fly parasitoid, diachasmimorpha tryoni.augmentative releases of parasitoid wasps are often used successfully for biological control of fruit flies in programs worldwide. the development of cheaper and more effective augmentative releases of the parasitoid wasp diachasmimorpha tryoni (cameron) (hymenoptera: braconidae) may allow its use to be expanded to cover queensland fruit fly, bactrocera tryoni (froggatt) (diptera: tephritidae), a serious pest of many vegetables and most fruit production in australia. this demands a fuller unders ...201324224552
genomic resources notes accepted 1 december 2013-31 january 2014.this article documents the public availability of (i) transcriptome sequence data, assembled and annotated contigs and unigenes, and blast hits from the queensland fruit fly, bactrocera tryoni; (ii) 75 single-nucleotide variants (snvs) from 454 sequencing of reduced representation libraries for phalangiidae harvestmen, megabunus armatus, megabunus vignai, megabunus lesserti, and rilaena triangularis; and (iii) expressed sequence tags from 454 sequencing of the lepidopterans lymantria dispar and ...201424655359
close genetic similarity between two sympatric species of tephritid fruit fly reproductively isolated by mating time.two sibling species of tephritid fruit fly, bactrocera tryoni and b. neohumeralis, occur sympatrically throughout the range of b. neohumeralis in australia. isolation between the two species appears to be maintained by a difference in mating time: b. tryoni mates at dusk, whereas b. neohumeralis mates during the middle of the day. a morphological difference in humeral callus color also distinguishes the two species. despite clear phenotypic evidence that b. tryoni and b. neohumeralis are distinc ...200010937263
genetic and molecular markers of the queensland fruit fly, bactrocera tryoni.twenty-six microsatellite markers, along with two restriction fragment length polymorphism (rflp) markers and three morphological markers, have been mapped to five linkage groups, corresponding to the five autosomes of the queensland fruit fly, bactrocera tryoni. all these molecular and genetic markers were genotyped in three-generation pedigrees. eight molecular markers were also localized to the salivary gland polytene chromosomes by in situ hybridization. this provides a substantial starting ...200614557395
the cryptochrome (cry) gene and a mating isolation mechanism in tephritid fruit flies.two sibling species of tephritid fruit fly, bactrocera tryoni and bactrocera neohumeralis, are differentiated by their time of mating, which is genetically determined and requires interactions between the endogenous circadian clock and light intensity. the cryptochrome (cry) gene, a light-sensitive component of the circadian clock, was isolated in the two bactrocera species. the putative amino acid sequence is identical in the two species. in the brain, in situ hybridization showed that cry is e ...200415611172
oviposition and mating behaviour of the queensland fruit-fly (dacus (strumeta) tryoni (frogg.)) and the solanum fruit fly (dacus (strumeta) cacuminatus (hering)). 195212977689
the evolutionary history of mariner-like elements in neotropical drosophilids.the evolutionary history of mariner-like elements (mles) in 49 mainly neotropical drosophilid species is described. so far, the investigations about the distribution of mles were performed mainly using hybridization assays with the mos1 element (the first mariner active element described) in a widely range of drosophilid species and these sequences were found principally in species that arose in afrotropical and sino-indian regions. our analysis in mainly neotropical drosophilid species shows th ...201121336962
tendency for upwind movement in the sibling fruit fly species, bactrocera tryoni and b. neohumeralis and their hybrids (diptera: tephritidae): influence of time of day, sex and airborne pheromone. 200312699539
the period gene in two species of tephritid fruit fly differentiated by mating behaviour.the period gene is important for the generation and maintenance of biological rhythms. it served as an ideal candidate for the investigation of the mating time isolation between two sibling queensland fruit fly species, bactrocera tryoni and bactrocera neohumeralis. we have isolated the homologues of the period gene in the two species, and show that their putative amino acid sequences are identical. no length polymorphism was detected in the thr-gly repeat region. per mrna expression, assayed in ...200212230541
towards a male-only release system for sit with the queensland fruit fly, bactrocera tryoni, using a genetic sexing strain with a temperature-sensitive lethal mutation.flies that are homozygous for the recessive autosomal mutation bent wings have a limited ability to fly and are less tolerant of high temperatures than normal flies in both the egg and puparial stages. the differences between the mutant and normal flies were found sufficient to be the basis of a genetic sexing strain. genetic sexing strains were created using translocations of the autosome bearing the wild-type allele of bent wings (chromosome 2) to the y chromosome, and crossing male flies carr ...200212484529
the scarlet eye colour gene of the tephritid fruit fly: bactrocera tryoni and the nature of two eye colour mutations.a homologue of the drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, bactrocera tryoni. the comparison of the b. tryoni and d. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the dna and the derived amino acid level, respectively. two allelic eye-colour mutations of b. tryoni, orange-eyes and lemon-eyes, have been recovered and found to be colocalized with the st gene. the st gene sequence in the two mutant s ...200312752660
olfaction in the queensland fruit fly, bactrocera tryoni. i: identification of olfactory receptor neuron types responding to environmental odors.the electroantennogram method was used to investigate the number of distinct olfactory receptor neuron types responding to a range of behaviorally active volatile chemicals in gravid queensland fruit flies, bactrocera tryoni. three receptor neuron types were identified. one type responds to methyl butyrate, 2-butanone, farnesene, and carbon dioxide; a second to ethanol; and a third to n-butyric acid and ammonia. the receptor neuron type responding to methyl butyrate, 2-butanone, farnesene, and c ...200111471941
the genome of the queensland fruit fly bactrocera tryoni contains multiple representatives of the mariner family of transposable elements.representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the queensland fruit fly bactrocera tryoni and its sibling species bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. three mariner types were also found in an additional tephritid, bactrocera jarvisi. using genomic library screening and inverse pcr, full-length elements representing the mellifera subfamily (b. tryoni.mar1) and the irritans subfamily ...200111520360
olfaction in the queensland fruit fly, bactrocera tryoni. ii: response spectra and temporal encoding characteristics of the carbon dioxide receptors.single-unit electrophysiology was used to record the nerve impulses from the carbon dioxide receptors of female queensland fruit flies, bactroera tryoni. the receptors responded to stimulation in a phasic-tonic manner and also had a period of inhibition of the nerve impulses after the end of stimulation. at high stimulus intensities. the cell responding to carbon dioxide was presented with a range of environmental odorants and found to respond to methyl butyrate and 2-butanone. the coding charac ...200111471942
suppression of cuelure attraction in male queensland fruit flies provided raspberry ketone supplements as immature adults.tephritid fruit flies are amongst the most damaging insect pests of horticulture globally. some of the key fruit fly species are managed using the sterile insect technique (sit), whereby millions of sterile males are released to suppress reproduction of pest populations. male annihilation technique (mat), whereby sex specific lures are used to attract and kill males, is often used to reduce wild male numbers before sit programs commence, providing released sterile males an increased numerical ad ...201728859132
seminal fluids mediate sexual inhibition and short copula duration in mated female queensland fruit flies.molecules in male seminal fluid transferred to female insects during mating can have potent effects on their subsequent sexual and reproductive behaviour. like many other tephritids, female queensland fruit flies (bactrocera tryoni) typically have diminished sexual receptivity after their first mating. also, copulations of females that do remate tend to be shorter than those of virgins. we here find that virgin females injected with small doses (0.1, 0.2 or 0.5 male equivalents) of extracts from ...200717187820
polymorphic microsatellite markers for population analysis of a tephritid pest species, bactrocera tryoni.to obtain a set of microsatellite markers for the queensland fruit fly bactrocera tryoni, a genomic library was screened with a number of simple repeat oligonucleotide probes. sequencing recovered 22 repeat loci. the microsatellite sequences were short, with repeat numbers ranging from five to 11. of these, 16 polymerase chain reaction (pcr) primer sets yielded amplifiable products, which were tested on 53 flies from five widely separated sites. all loci showed polymorphism in the population sam ...19989819903
bactrocera tryoni and closely related pest tephritids--molecular analysis and prospects for transgenic control strategies.bactrocera tryoni is a serious pest of horticulture in eastern australia. here we review molecular data relevant to pest status and development of a transformation system for this species. the development of transformation vectors for non-drosophilid insects has opened the door to the possibility of improving the sterile insect technique (sit), by genetically engineering factory strains of pest insects to produce male-only broods. transposition assays indicate that all five of the vectors curren ...200414871613
mitotic and polytene chromosome analyses in the queensland fruit fly, bactrocera tryoni (diptera: tephritidae).the queensland fruit fly, bactrocera tryoni, like the mediterranean fruit fly, ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a xx/xy sex chromosome pair. characteristic features of each chromosome are described. chromosomal homology between b. tryoni and c. capitata has been determined by comparing chromosome banding pattern and in situ hybridisation of cloned genes to polytene chromosomes. although the evidence indicates that a number of c ...19989796100
the queensland fruit fly, bactrocera tryoni, contains multiple members of the hat family of transposable elements.members of the hat transposable element family are mobile in non-host insect species and have been used as transformation vectors in some of these species. we report that the queensland fruit fly, bactrocera tryoni, contains at least two types of insect hat elements called homer and a homer-like element (hle). the homer element is 3789 bp in size and contains 12-bp imperfect inverted terminal repeats. the homer element contains a long open reading frame (orf) that encodes a putative transposase. ...199910620037
the bactrocera tryoni homologue of the drosophila melanogaster sex-determination gene doublesex.a homologue of the bifunctional sex-determining gene, doublesex (dsx), has been identified in the tephritid fruit fly, bactrocera tryoni, and has been found to be expressed in a sex-specific manner in adult flies. the male- and female-specific cdnas are identical at their 5' ends but differ at their 3' ends and appear to be the products of alternate splicing. the level of identity of the sex-specific dsx proteins of b. tryoni with the d. melanogaster dsx proteins, across the region corresponding ...19989723873
mobility of hat transposable elements in the old world bollworm, helicoverpa armigera.the p, hobo, hermes, minos and mariner transposable elements have been successfully used as gene vectors to achieve genetic transformation of drosophila melanogaster. the hobo transposable element of d. melanogaster, a member of the hat family of transposable elements, can also transpose in other dipteran species including the house fly musca domestica and the queensland fruit fly, bactrocera tryoni. another hat element, hermes, can also transpose in a wide range of insect species. we report her ...19968933173
australian endemic pest tephritids: genetic, molecular and microbial tools for improved sterile insect technique.among australian endemic tephritid fruit flies, the sibling species bactrocera tryoni and bactrocera neohumeralis have been serious horticultural pests since the introduction of horticulture in the nineteenth century. more recently, bactrocera jarvisi has also been declared a pest in northern australia. after several decades of genetic research there is now a range of classical and molecular genetic tools that can be used to develop improved sterile insect technique (sit) strains for control of ...201425470996
the likely fate of hybrids of bactrocera tryoni and bactrocera neohumeralis.bactrocera tryoni (froggatt) and b. neohumeralis (hardy) (diptera: tephritidae) are sympatric species which hybridise readily in the laboratory yet remain distinct in the field. b. tryoni mates only at dusk and b. neohumeralis mates only during the day, but hybrids can mate at both times. we investigated the inheritance of mating time in successively backcrossed hybrid stocks to establish whether mating with either species is more likely. the progeny of all backcrosses to b. tryoni mated only at ...200312714981
plant-mediated female transcriptomic changes post-mating in a tephritid fruit fly, bactrocera tryoni.female post-mating behaviors are regulated by complex factors involving males, females, and the environment. in insects, plant secondary compounds that males actively forage for, may indirectly modify female behaviors by altering male behavior and physiology. in the tephritid fruit fly, bactrocera tryoni, females mated with males previously fed on plant-derived phenylpropanoids (="lures" based on usage in tephritid literature), have longer mating refractoriness, greater fecundity, and reduced lo ...201829220418
effect of body size, age, and premating experience on male mating success in bactrocera tryoni (diptera: tephritidae).variation in male body size, age, and prior sexual experience may all influence male mating success in tephritid fruit flies. bactrocera tryoni (froggatt) is an australian pest tephritid for which the sterile insect technique (sit) is being actively pursued, and for which information on what makes males more competitive is urgently needed. pair-wise competitive mating trials were run using laboratory-reared flies in walk-in field cages, evaluating young, large, and virgin b. tryoni males against ...201729048584
two gut-associated yeasts in a tephritid fruit fly have contrasting effects on adult attraction and larval survival.yeast-insect interactions have been well characterized in drosophilid flies, but not in tephritid fruit flies, which include many highly polyphagous pest species that attack ripening fruits. using the queensland fruit fly (bactrocera tryoni) as our model tephritid species, we identified yeast species present in the gut of wild-collected larvae and found two genera, hanseniaspora and pichia, were the dominant isolates. in behavioural trials using adult female b. tryoni, a fruit-agar substrate ino ...201728836040
semiochemical mediated enhancement of males to complement sterile insect technique in management of the tephritid pest bactrocera tryoni (froggatt).queensland fruit fly, bactrocera tryoni (froggatt), is the most significant pest of australia's $9 billion horticulture industry. the sterile insect technique (sit) and cue-lure (a synthetic analogue of raspberry ketone (rk))-based male annihilation technique (mat) are two of the most effective management tools against this pest. however, combining these two approaches is considered incompatible as mat kills sterile and 'wild' males indiscriminately. in the present study we tested the effect of ...201729042676
revised distribution of bactrocera tryoni in eastern australia and effect on possible incursions of mediterranean fruit fly: development of australia's eastern trading block.bactrocera tryoni (froggatt) (diptera: tephritidae), commonly called 'queensland fruit fly' in australia, and mediterranean fruit fly (ceratitis capitata wiedemann) (diptera: tephritidae) are the two most economically important fruit fly in australia with b. tryoni in the east and mediterranean fruit fly in the west. the two species coexisted for several decades, but it is believed that b. tryoni displaced mediterranean fruit fly. in southeastern australia, this was deemed inadequate for export ...201729040591
potential impacts of climate change on habitat suitability for the queensland fruit fly.anthropogenic climate change is a major factor driving shifts in the distributions of pests and invasive species. the queensland fruit fly, bactrocera tryoni froggatt (qfly), is the most economically damaging insect pest of australia's horticultural industry, and its management is a key priority for plant protection and biosecurity. identifying the extent to which climate change may alter the distribution of suitable habitat for qfly is important for the development and continuation of effective ...201729026169
efficacy of chemicals for the potential management of the queensland fruit fly bactrocera tryoni (froggatt) (diptera: tephritidae).this study investigated alternative in-field chemical controls against bactrocera tryoni (froggatt). bioassay 1 tested the mortality of adults exposed to fruit and filter paper dipped in insecticide, and the topical application of insecticide to adults/fruit. bioassay 2 measured the mortality of adults permitted to oviposit on fruit dipped in insecticide and aged 0, 1, 3, or 5 days, plus the production of offspring. bioassay 3 tested infested fruit sprayed with insecticide. the field bioassay tr ...201728486404
screening mitochondrial dna sequence variation as an alternative method for tracking established and outbreak populations of queensland fruit fly at the species southern range limit.understanding the relationship between incursions of insect pests and established populations is critical to implementing effective control. studies of genetic variation can provide powerful tools to examine potential invasion pathways and longevity of individual pest outbreaks. the major fruit fly pest in eastern australia, queensland fruit fly bactrocera tryoni (froggatt), has been subject to significant long-term quarantine and population reduction control measures in the major horticulture p ...201728428851
effect of physiological and experiential state ofbactrocera tryoni flies on intra-tree foraging behavior for food (bacteria) and host fruit.using caged host trees on which we manipulated food and oviposition sites, we investigated the foraging behavior of individually-releasedbactrocera tryoni (diptera: tephritidae) females in relation to state of fly hunger for protein, presence or absence of bacteria as a source of protein, degree of prior experience with host fruit, and quality of host fruit for oviposition. one aim was to evaluate whether it is immature or matureb. tryoni females that are responsible for initially inoculating ho ...199128313268
cytogenetic and symbiont analysis of five members of the b. dorsalis complex (diptera, tephritidae): no evidence of chromosomal or symbiont-based speciation events.the bactrocera dorsalis species complex, currently comprising about 90 entities has received much attention. during the last decades, considerable effort has been devoted to delimiting the species of the complex. this information is of great importance for agriculture and world trade, since the complex harbours several pest species of major economic importance and other species that could evolve into global threats. speciation in diptera is usually accompanied by chromosomal rearrangements, part ...201526798263
automated locomotor activity monitoring as a quality control assay for mass-reared tephritid flies.the sterile insect technique (sit) requires vast numbers of consistently high quality insects to be produced over long periods. quality control (qc) procedures are critical to effective sit, both providing quality assurance and warning of operational deficiencies. we here present a potential new qc assay for mass rearing of queensland fruit flies (bactrocera tryoni froggatt) for sit; locomotor activity monitoring. we investigated whether automated locomotor activity monitors (lams) that simply d ...201423589415
optimizing irradiation dose for sterility induction and quality of bactrocera tryoni.the current study is an important step toward calibrating, validating, and improving irradiation methods used for bactrocera tryoni (froggatt) sterile insect technique (sit). we used routine international atomic energy agency/u.s. department of agriculture/food and agriculture organization quality control tests assessing percentage of emergence, flight ability, sex ratio, mortality under stress, reproductive sterility, and sexual competitiveness, as well as a nonstandard test of longevity under ...200919886443
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