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digestive enzymes of three teleost fishes.the activity of amylase, sucrase, protease and lipase has been examined in wallago attu, clarias batrachus and labeo rohita. the optimum ph value for carbohydrases ranges from 5.0 to 7.0 and that for trypsin between ph 6.8 and 7.8. lipase is active at a slightly more alkaline medium. the optimum ph for a given enzyme varies in different sections of the alimentary canal of the same fish and also from species to species. variations are also found in the optimum substrate concentration for a given ...197514477
seasonal changes in cholesterol content of the muscle of the cat-fish clarias batrachus l. 1979478590
a histochemical study of the mucous cells in the bucco-pharyngeal region of four indian freshwater fishes in relation to their origin, development, occurrence and probable functions.observations on the origin, development and occurrence of mucous cells in the bucco-pharyngeal region of labeo rohita (ham.), cirrhinus mrigala (ham.), catla catla (ham.) and clarias batrachus (linn.) have been made histochemically, by pas technique. the mucous cells have been found to develop from ordinary epithelial cells of the stratum malpighii which is situated immediately above the basement membrane. the different stages in the development of mucous cells are: 1. formation stage: some of t ...1975811054
cyclic changes in serum cholesterol levels of fresh water cat fish clarias batrachus. 1976952092
histamine content of the digestive tract of a catfish clarias batrachus (linn.) in relation to spawning, sex and seasonal variation.histamine content of the digestive tract of a catfish clarias batrachus has been estimated biologically on the atropinized guineapig ileum. a significant decrease of histamine content has been observed with the decrease of gonosomatic index (g.s.i.) during breeding season in female fishes, while no such type of change in histamine content of male fishes during breeding season has been noted. in the summer histamine content of both the sexes was signivicantly lowered in comparison with the histam ...19751158436
thyroid physiology impairment by malathion in the freshwater catfish clarias batrachus.effects of malathion on serum and glandular thyroxine (t4), triiodothyronine (t3), the t3/t4 ratio, peroxidase activity, and extrathyroidal conversion of t4 to t3 have been studied in the freshwater catfish clarias batrachus during the prespawning and spawning phases of its annual reproductive cycle. malathion inhibited t4 secretion in the kidney, but accelerated t4 synthesis in the pharyngeal thyroid. this pesticide also inhibited the extrathyroidal conversion of t4 to t3 in serum during both t ...19921385075
oocyte maturation in clarias batrachus: in vitro effect of various gonadotropins and homologous pituitary homogenate.the effects of five different gonadotropins and homologous pituitary homogenate (hp) on germinal vesicle breakdown (gvbd) were investigated in vitro using folliculated oocytes of clarias batrachus. among all the gonadotropins, salmon gonadotropin (sg-g100) was the most potent in vitro inducer of oocyte maturation. at concentrations of 1, 0.1, 0.01 and 0.001 microgram/ml it induced 86.98 +/- 2.71, 68.74 +/- 2.85, 44.56 +/- 1.75 and 25.90 +/- 2.36% gvbd. next to sg-g100 in inducing gvbd was lutein ...19921512031
pesticides induced changes in circulating thyroid hormones in the freshwater catfish clarias batrachus.1. effects of endosulfan, malathion and carbaryl on circulating t4, t3 and t3/t4 ratio in the freshwater catfish, c. batrachus have been studied during reproductively active phases, i.e. vitellogenic and post-vitellogenic phases of its annual reproductive cycle. 2. endosulfan provoked a significant increase in t4 and a decrease in t3 as well as the t3/t4 ratio. 3. malathion decreased t3 and t3/t4 ratio without altering the level of t4 in circulation. 4. carbaryl caused a decline in the level of ...19911677838
outer membrane protein profiles of edwardsiella ictaluri from fish.outer membrane proteins (omp) prepared with sodium n-lauroyl sarcocinate (sls) from 33 edwardsiella ictaluri isolates from fish were examined by electrophoresis. twenty-eight isolates from channel catfish (ictalurus punctatus) had similar omp profiles. ten bands (71 kilodaltons [kd] to 19.5 kd) were identified in all isolates from channel catfish. one major 35-kd protein comprised most of the protein content of the outer membrane of isolates from channel catfish. differences existed among isolat ...19901689127
carbaryl-induced thyroid dysfunction in the freshwater catfish clarias batrachus.freshwater female catfish, clarias batrachus, were exposed to sublethal concentrations of carbaryl (12 mg/liter for 96 hr and 5 mg/liter for 16 days) during the prespawning and spawning phases of their annual reproductive cycle. the effects on the levels of thyroxine (t4) and triiodothyronine (t3), the t3/t4 ratio, and the extrathyroidal conversion of t4 to t3 were studied in serum, pharyngeal thyroid, and anterior and posterior kidney. carbaryl exposure for 96 hr suppressed t4 but elevated t3 a ...19911907904
effect of endosulfan on thyroid physiology in the freshwater catfish, clarias batrachus.effects of endosulfan on thyroid physiology have been studied in the female freshwater catfish. clarias batrachus during the pre-spawning and spawning phases of its annual reproductive cycle. effects of endosulfan varied with the length (96 h and 16 days) of exposure, reproductive status of the fish and organ. the 96-h endosulfan exposure significantly increased the level of thyroxine (t4) in serum and pharyngeal thyroid follicles concurrent with induction of peroxidase activity. however, triiod ...19912031252
influence of human urinary erythropoietin and l-thyroxine on blood morphology and energy reserves in two tropical species of fed and starved teleosts.erythropoietic responses of fed and starved species of teleosts, viz., clarias batrachus and heteropneustes fossilis, to human urinary erythropoietin and thyroxine have been examined. the effects of these hormones on energy reserves have also been evaluated. twenty-four c. batrachus were divided into two groups: half were fed regularly; the remaining fish were starved 20 days. on the 21st day each group was further divided into three subgroups of four each and received either saline, thyroxine ( ...19892583468
biochemical compartmentation of gluconeogenic enzymes in fish tissues.compartmentation of liver, kidney muscle and gill tissues in relation to glucose-6-phosphatase and fructose 1,6-diphosphatase was examined in the fishes labeo rohita, clarias batrachus and channa punctatus. the anterior region of the right and left lobes of the liver contained the maximum of fructose 1,6-diphosphatase and glucose-6-phosphatase, while the minimum was in the right and left lobes of gill tissue. herbivore fish had the highest gluconeogenic enzyme content followed by carnivore and p ...19863030045
differential distribution of hexokinase in vital organs of three teleost fishes.the phylogeny has influenced the distribution of hexokinase in various tissues of three teleosts, viz., labeo rohita, clarias batrachus and channa punctatus, which differ significantly in a number of parameters like growth rate, feeding habits and ability to get oxygen. among the organs investigated for hexokinase, the anterior region of the right lobe of the liver showed higher activity than the posterior region, followed by the anterior and posterior regions of the left lobe. the middle region ...19883250207
effect of melatonin and 5-methoxytryptamine on sex steroids and thyroid hormones during the prespawning phase of the annual reproductive cycle in the freshwater teleost, clarias batrachus.melatonin and 5-methoxytryptamine have been studied in the freshwater teleost clarias batrachus during the prespawning period. the data have provided evidence of the inhibitory effects on sex steroids and thyroid hormones when the pineal indoleamines melatonin and 5-methoxytryptamine (5-mt) are administered intramuscularly daily in the afternoon for 27 days. the different dose levels had different effects. the higher dose levels of melatonin showed the initiation of counter inhibitory impacts on ...19873478467
impact of sex steroids on plasma t3 and t4 levels in the freshwater catfish clarias batrachus (linn.). 19863570368
effect of pinealectomy on thyroid hormone (t4 and t3) levels in plasma during annual reproductive cycle in the freshwater catfish, clarias batrachus.thyroxine and triiodothyronine (t3) were measured by radioimmunoassay in serum and thyroid gland of the freshwater teleost clarias batrachus. pinealectomy increased the glandular levels of the thyroid hormones but decreased the plasma levels of t4 concomitantly with the increase of t3 levels. most of the effects of pinealectomy on thyroid hormones were restricted to the gonadal development periods. pinealectomy has shown no significant impact on thyroid hormones during the other periods of the r ...19873668789
redescription of bovienia serialis (bovien, 1926) (cestoidea: caryophyllidea) from the catfish clarias batrachus (l.) in india. 19724641408
lytocestus longicollis sp. nov. (cestoidea: caryophyllidea) from the catfish clarias batrachus (l.) in india. 19734775760
ddt toxicity: gluconeogenic enzymes and non-specific phosphomonoesterases in three teleosts.in 3 fish species (labeo rohita, clarias batrachus and channa punctatus) subjected to a sublethal concentration (0.035 mg/l) of ddt continuously for 20 days both hepatic and renal acid and alkaline phosphatases were elevated, whereas the rise in glucose-6-phosphatase and fructose-1,6-diphosphatase was restricted to hepatic tissue. the variations in phosphatase levels were more pronounced in l. rohita than in the other two species.19826294922
induction of ovulation and spawning in the catfish, clarias batrachus (l.) by prostaglandin f2 alpha.administration of low doses of prostaglandin f2 alpha (pgf2 alpha), i.e. 10 and 100 ng/g b.wt, was ineffective in inducing ovulation and spawning in mature catfish. however, doses of 200 ng/g b.wt of pgf2 alpha elicited partial response, and 500 ng/g b.wt of pgf2 alpha induced profuse ovulation and spawning similar to that induced by administration of human chorionic gonadotropin (hcg).19836572600
alterations in enzymes in an indian catfish, clarias batrachus (linn.), exposed to vanadium. 19957742637
antibody dependent haemolysin, complement and opsonin in sera of a major carp, cirrhina mrigala and catfish, clarias batrachus and heteropneustes fossilis.the present communication is a continuation of our earlier study on the natural serum haemagglutinin/lectins of cirrhina mrigala, clarias batrachus and heteropneustes fossilis. sera of cirrhina mrigala, belonging to the major carp family, could not only agglutinate heterologous rabbit erythrocytes, but also lyse them spontaneously. this lysis of rabbit rbc by cirrhina mrigala sera was calcium ion dependent and heat sensitive, indicating thereby that the haemolysis was mediated by the fish serum ...19938281746
comparison of the sublethal effect of mercury and lead on visceral dehydrogenase system in three inland teleosts.the sublethal effect of mercury and lead was investigated on visceral (liver, muscle, gill, kidney and brain) succinic, malic and lactic dehydrogenases in labeo rohita, clarias batrachus and chana punctatus in acute experiments. the highest decrease of succinic, malic and lactic dehydrogenases was recorded in the hepatic tissue in comparison to muscle, brain, kidney and gill. this decrease was greater in l. rohita than in c. batrachus or in c. punctatus. mercury was more effective than lead. mar ...19938329378
observations on the function of mucous cells in the epidermis of the cat-fish clarias batrachus exposed to sodium dodecyl sulfate.response of mucous cells, in the epidermis of the catfish clarias batrachus, to a sublethal concentration of sodium dodecyl sulfate was studied and correlated with the alteration in the status of their secretory activity after different durations of the treatment. at 4h, 8h, 24h, 48h and 72h of the detergent treatment, most mucous cells attained voluminous dimensions and appeared closely approximating to or even overlapping the adjacent ones. at 12h, 36h, and 60h, in contrast, the mucous cells a ...19938397895
sublethal effect of mercury and lead on monoamine oxidase in different regions of the brain in three freshwater teleosts.effect of mercury and lead at 24 and 48 h was investigated on monoamine oxidase (mao) activity in different regions of the brain (telencephalon, cerebellum, diencephalon and medulla oblongata) in labeo rohita, ham., clarias batrachus, l. and channa punctatus, bloch. highest rise in mao activity was recorded in telencephalon with mercury followed by lead. maximum variations in the level of mao activity in different regions of the brain were recorded at 24 h exposure. the observed alterations were ...19958606989
direct mutagenicity of the polycylic aromatic hydrocarbon-containing fraction of smoked and charcoal-broiled foods treated with nitrite in acid solution.the polycyclic aromatic hydrocarbons (pah) containing fractions of smoked and charcoal-broiled foods, namely, sheat fish (kytopterus apogon), mimrow (crossocheilus reba), freshwater catfish (clarias batrachus), chicken wings, rice pork sausage and pork, in addition to naphthalene, acenaphthene, anthracene, phenanthrene, fluoranthene, pyrene, benz[a]anthracene, naphthacene, benzo[a]pyrene, benzo[e]pyrene, 9,10-dimethyl-1,2-benzanthracene, dibenz[ah]anthracene, benzo[ghi]perylene and coronene, wer ...19979146734
vanadium toxicology--an assessment of general health, haematological aspects and energy response in an indian catfish clarias batrachus (linn).the pervasive occurrence of vanadium in nature and its use in various industrial processes has increased its inputs in the environment. this has prompted us to elucidate the impact of vanadium on aquatic environment, the primary body for industrial effluent discharge. the energy response of the fish, clarias batrachus, its haematological status including haemoglobin (hb), haematocrit (ht), leutocrit (lt), mean corpuscular haemoglobin (mch), mean corpuscular volume (mcv), mean corpuscular haemogl ...19989542062
characterization of mboi satellites in cirrhina mrigala and clarias batrachus (pisces).we have cloned and characterized two highly reiterated, tandemly repeated, and a+t rich mboi dna fragments, one in cirrhina mrigala (cyprinidae), with a monomer size of 266 bp, and one in clarias batrachus (clariidae), with a monomer size of 227 bp. the mboi fragment in c. mrigala is species-specific and absent in other carps, such as catla catla and labeo rohita. the mboi fragment in c. batrachus was also present in two other catfishes tested, namely clarias gariepinus and heteropneustes fossil ...19989549057
partial purification of maturation-promoting factor from catfish, clarias batrachus: identification as the histone h1 kinase and its periodic activation.maturation-promoting factor (mpf) has been demonstrated in the 100,000 g supernatant of 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (17 alpha, 20 beta-dp)-induced catfish, clarias batrachus oocytes using deae-cellulose and sephadex g-200 chromatography. partially purified mpf molecule eluted as a single peak on sephadex g-200 with molecular mass of approximately 200 kda in native page. sds-page analysis showed the presence of five proteins of 32, 34, 45, 46 and 48 kda. antibody against the pstai ...19989827048
effect of acute exposure of the organophosphate insecticide rogor on some biochemical aspects of clarias batrachus (linnaeus).indian catfish (clarias batrachus), a common fish of commercial importance, was exposed to a sublethal concentration (1/3 of 96 h lc50 value) of rogor for periods ranging from 24 to 192 h. the changes in glycogen, lactate, lactate dehydrogenase, and glycogen phosphorylase (a, ab) contents of muscle tissue were studied. a gradual decrease in muscle glycogen and an increase in lactate contents were observed. the activity level of lactate dehydrogenase showed a sharp rise initially for 48 h followe ...19999931229
role of thyroidal and testicular hormones in regulation of tissue respiration in male air-breathing fish, clarias batrachus (linn.).in vivo and in vitro effects of thyroidal hormones (mit, dit, t3, t4), propyl thiouracil (ptu), testosterone and cyproterone acetate were studied on the rate of tissue (liver, muscle, kidney and brain) respiration of adult male c. batrachus during winter and summer/rainy seasons. monoiodotyrosine (mit) and diiodothyrosine (dit) increased the respiratory rate in a dose-dependent and temperature-independent manner. triiodothyronine (t3) and thyroxine (t4) stimulated tissue respiration during summe ...200011215315
pesticide induced alterations of non-protein nitrogenous constituents in the serum of a fresh water cat fish, clarias batrachus (linn.).a freshwater cat fish was exposed to sublethal concentrations of two pesticides--carbaryl, a carbamate and phorate, an organophosphorus pesticide for 24, 72, 120 and 168 hr. the alterations in the serum profile of non protein nitrogen compounds demonstrated an increase in urea, uric acid and creatinine throughout the experimental period.200011324161
effects of ovine lh, gh and prolactin, and testosterone on serum testosterone and estradiol-17 beta levels, and seminal vesicle and testicular activity in the catfish clarias batrachus (l.).intraperitoneal administrations of testosterone (0.5 microgram/g body wt), and ovine lh (1.0 microgram/g body wt), gh (5 micrograms/g body wt) and prolactin (10 micrograms/g body wt) daily for 7 days during early prespawning phase (may) in c. batrachus produced varied effects on seminal vesicle (svsi) and testicular (gsi) weights and biochemical correlates. testosterone and lh treatments significantly increased serum testosterone level and concentrations of total proteins, fructose, hexosamines ...200111349526
dietary bovine lactoferrin induces changes in immunity level and disease resistance in asian catfish clarias batrachus.the effects of including bovine lactoferrin (lf) in the diet of the asian catfish (clarias batrachus) on specific and non-specific immunity as well as disease resistance were investigated. the catfish were fed four different diets for 2 weeks: a commercial diet as control and the same diet supplemented with 50, 100 and 200mg bovine lf/kg feed. after 1 and 2 weeks, serum bacterial agglutination titre against aeromonas hydrophila as a measure of specific immunity; natural serum haemolysin titre, l ...200312842607
studies on lethal concentrations and toxicity stress of some xenobiotics on aquatic organisms.three widely used xenobiotics pentachlorophenol (pcp), 2,4-dichlorophenoxyacetic acid (2,4-d) and 2-chloro-2,6-diethyl-n-(butoxymethyl) acetanilide (butachlor) are evaluated for acute toxicity and stress behavior on freshwater fish (heteropneustes fossilis, clarias batrachus, channa punctatus) and mosquito larvae (culex pipiens fatigans). the experiment was carried out by medium treatment using intermittent flow-through system. median lethal concentrations (lc50) were calculated by probit analys ...200414761696
integrated vermi-pisciculture--an alternative option for recycling of solid municipal waste in rural india.vermicomposts as a biofertilizer can be a great option for pond manuring as they never cause any long term harm to the soil like chemical fertilizer. in this study vegetable and horticulture waste was used as an important media for vermiculture. three separate cemented tanks (6 m(3) each) were used in the system as control tank, vermicompost fertilized tank and inorganic fertilizer manured tank. monoculture of fish was carried out with cat fish, clarias batrachus. the produced earthworms were us ...200414987723
purification and properties of cytosolic alanine aminotransferase from the liver of two freshwater fish, clarias batrachus and labeo rohita.cytosolic alanine aminotransferase (c-aat) was purified up to 203- and 120-fold, from the liver of two freshwater teleosts clarias batrachus (air-breathing, carnivorous) and labeo rohita (water-breathing, herbivorous), respectively. the enzyme from both fish showed similar elution profiles on a deae-sephacel ion exchange column. sds-page of purified enzymes revealed two subunits of 54 and 56 kda, in both fish. the apparent km values for l-alanine were 18.5+/-0.48 and 23.55+/-0.60 mm, whereas for ...200414990216
evaluation of biofilm of aeromonas hydrophila for oral vaccination of clarias batrachus--a carnivore model.biofilm of aeromonas hydrophila was evaluated for oral vaccination of walking catfish (clarias batrachus l.). fish were fed with fish paste incorporating biofilm (bf) or free cells (fc) of a. hydrophila for 20 days and monitored for serum antibody production up to 60 days post-vaccination. serum agglutinating antibody titre and relative percent survival (rps) following challenge were found to be significantly higher in catfish fed with bf vaccine compared to that with fc.200415110335
one step purification and partial characterisation of serum immunoglobulin from asiatic catfish (clarias batrachus l.). 200415312666
effect of acute exposure of sodium arsenite (na3 aso3) on some haematological parameters of clarias batrachus (common indian cat fish) in vivo.the effect of acute arsenic exposure in the form of sodium arsenite (na3aso3) were studied on haematological parameters of clarias batrachus. three subleathal concentrations of sodium arsenite (5 ppm, 10 ppm, 15 ppm) were exposed to experimental set of 10 fishes in a glass aquarium filled with 40 l water. the parameters screened after 120 hrs exposure for each experimental set were hb concentration, tec, pcv and mcv mch and mchc were also calculated. statistical analysis shows a significant fall ...200315315139
scaling effects on metabolism of a teleost.scaling effects on citrate synthase (cs), glucose-6-phosphate dehydrogenase (g6-pdh), rna. rna/dna ratio and protein contents of brain, liver and skeletal muscle were studied in a teleost, clarias batrachus. the activity of white skeletal muscle cs decreased significantly as a function of increasing body mass of the fish. it shows that the fulfilment of energy demand in white skeletal muscle is not dependent on aerobic metabolism. the activity of liver g6-pdh decreased with the increasing body m ...200415559933
effects of cyclophosphamide on the immune system and disease resistance of asian catfish clarias batrachus.cyclophosphamide (cyp), a multifunctional alkylating agent is known as a potent immunosuppressor in endotherms. here, an experiment was conducted in an ectothermic asian catfish species, clarias batrachus to investigate its effect on non-specific and specific immunity as well as disease resistance against a common bacterial pathogen challenge. cyp was intraperitoneally injected for 3 consecutive days at a rate of 200 mgkg(-1) body weight. after 72 h post-injection, control and cyp-treated fish w ...200515863012
characterization of a galactose binding serum lectin from the indian catfish, clarias batrachus: possible involvement of fish lectins in differential recognition of pathogens.a lectin with molecular mass around 200 kda was isolated from the serum of the indian catfish clarias batrachus. the bioactivity of this serum lectin was ca2+ and ph dependent. the lectin appeared to be specific for alpha-methyl galactose and sialoglycoproteins like porcine and bovine submaxillary mucin and could agglutinate human, rabbit, mice, rat and chicken erythrocytes. this fish lectin was able to specifically agglutinate different gram negative bacteria. when it was checked against differ ...200515979414
evidence of apoptotic effects of 2,4-d and butachlor on walking catfish, clarias batrachus, by transmission electron microscopy and dna degradation studies.apoptosis or programmed cell death is characterized morphologically by chromatin condensation, cell shrinkage, fragmentation of the nucleus and cytoplasm, and consequently formation of apoptotic bodies. it has also been best characterized by the cleavage of dna into nucleosomal size fragments of 180-200 bp or multiples of the same. contrary to this, under extreme conditions, the cells were found to show adaptive response to apoptosis and unable to regulate their own death; necrosis is therefore ...200616137705
high dietary vitamin c affects growth, non-specific immune responses and disease resistance in asian catfish, clarias batrachus.studies were conducted to determine the immunomodulatory effects of high dietary ascorbic acid (vitamin c) on growth, serum concentration, non-specific immune response and disease resistance of a commercially important asian catfish, clarias batrachus. four practical diets were formulated to contain 0, 500, 1000 and 2000 mg ascorbic acid (aa) equivalent/kg diet, supplied as l-ascorbyl-2-polyphosphate (lapp) and were fed for 2, 4, 6 and 8 weeks. each diet was fed to triplicate groups of catfish w ...200516311902
dietary beta-1,3 glucan potentiates innate immunity and disease resistance of asian catfish, clarias batrachus (l.).this study investigated the effects of short and prolonged administration of a yeast beta-glucan on non-specific immune parameters, growth rate and the disease resistance of asian catfish, clarias batrachus. fish fed with a basal diet (control) and test diet (basal diet supplemented with 0.1% glucan) for 1, 2 and 3 weeks were assayed for superoxide production, serum myeloperoxidase (mpo) content, natural haemagglutinin level, complement and lysozyme activities. fish were weighed at weekly interv ...200616436120
study of serum cholinesterase levels in fish clarias batrachus (linn.) exposed to pesticides carbaryl and phorate.study of the serum cholinesterase levels (sche) in the freshwater catfish clarias batrachus when exposed to sub lethal dose of pesticides phorate and carbamate, was attempted in this paper. sche levels decreased in the fish exposed to both the pesticides, the depletion being more pronounced with phorate. these results can be due to impairment of nervous system, liver damage as well as myocardial infarction. similar findings were not only reported in experimental organisms but also found in human ...200416649625
perturbations in the catfish immune responses by arsenic: organ and cell specific effects.the present study was an attempt to elucidate the effect of non-lethal arsenic (as) exposure (1/10 lc50) on different immunologically important organs and cells of a catfish. clarias batrachus l. were exposed to arsenic trioxide for different time intervals, which resulted in significant, time-dependent changes in total head kidney and splenic leucocyte count (p<0.05) and reduction in the organosomatic indices (p<0.05) of these two important immunocompetent organs. routine histological studies r ...200616765097
dietary immunostimulants influence specific immune response and resistance of healthy and immunocompromised asian catfish clarias batrachus to aeromonas hydrophila infection.in order to determine the efficacy and immunoreversal effect of the 4 dietary immunomodulators, viz. lactoferrin, beta-1,3 glucan, levamisole and vitamin c, on disease resistance of a commercially important catfish, clarias batrachus, fish were fed diets supplemented with various levels of these substances in 2 subgroups, healthy and immunocompromised, during a 30 d trial. an artificial immunosuppressive state was induced by giving 3 intraperitoneal (i.p.) injections of cyclophosphamide (cyp) at ...200616875392
correlation between biochemical properties and adaptive diversity of skeletal muscle myofibrils and myosin of some air-breathing teleosts.functional properties of myofibrils and relative stability of myosin of five teleosts channa punctata, clarias batrachus, m astacembalus armatus, labeo rohita and catla catla adapted to different breathing modes were compared. myofibrillar contractility and m-atpase of air-breathing organ (abo) possessing c.punctata and c. batrachus were low and least affected by ph in the range of 7.1-8.5. however, their myosin isoforms were relatively thermostable, more soluble at sub-neutral ph values, betwee ...200617133765
long-term exposure to arsenic affects head kidney and impairs humoral immune responses of clarias batrachus.the present study was aimed at determining the effects of long-term arsenic exposure on the head kidney (hk) and ensuing humoral immune responses in clarias batrachus l. long-term exposure (150 days) to non-lethal concentrations of arsenic (42.42 microm) resulted in significant time-dependent alterations in hk cell number eventually affecting the hk somatic index. prolonged exposure to arsenic also suppressed hk-b cell proliferation and led to significant reduction in serum immunoglobulin levels ...200717182119
genotypic characterization of listeria spp. isolated from fresh water fish.a total of 200 samples (muscles and viscera, 100 of each) of fresh water fish, walking catfish (clarias batrachus) were screened for listeria spp. all the samples were subjected to a two-step enrichment followed by plating on selective media. confirmation of the isolates was on the basis of biochemical characters, haemolysis on blood agar and christie, atkins, munch petersen test. a total of 39 isolates of listeria spp. were recovered. of these 26 (67%), 8 (21%), 3 (8%) and 2 (5%) were listeria ...200717182144
an attenuated plasmid-cured strain of aeromonas hydrophila elicits protective immunity in clarias batrachus l.wild type aeromonas hydrophila (strain ao1) isolated from the lesions of ulcerative disease syndrome (uds) affected fish bears a 21 kb virulence plasmid. with plasmid curing the isolates became attenuated and failed to induce fatal haemorrhagic ulcers in fish. the objective of the present work was to check the immunogenicity of these plasmid-cured derivatives and determine whether such strains could be used as candidate antigens for eliciting protective immunity to a. hydrophila infections in th ...200717208455
the status of quadriacanthus paperna, 1961 and anacornuatus dubey et al., 1991 (monogenoidea: dactylogyridae) with redescription of q. kobiensis ha ky, 1968, new geographical records for q. bagrae paperna, 1979 and q. clariadis paperna, 1961 from india and a note on speciation in monogenoidea.quadriacanthus paperna, 1961 is revised and emended. q. kobiensis ha ky, 1968 is redescribed from clarias batrachus, and q. bagrae paperna, 1979 and q. clariadis paperna, 1961 are recorded from c. gariepinus, both from the river gomti, lucknow. india is a new geographical record for q. bagrae and q. clariadis. anacornuatus dubey et al., 1991 is considered a junior subjective synonym of quadriacanthus paperna, 1961. quadriacanthus spp. are more specific to their hosts (micro-habitats) than geogra ...200717234447
cell volume regulation in the perfused liver of a freshwater air-breathing cat fish clarias batrachus under aniso-osmotic conditions: roles of inorganic ions and taurine.the roles of various inorganic ions and taurine, an organic osmolyte, in cell volume regulation were investigated in the perfused liver of a freshwater air-breathing catfish clarias batrachus under aniso-osmotic conditions. there was a transient increase and decrease of liver cell volume following hypotonic (-80 mosmol/l) and hypertonic (+80 mosmol/l) exposures,respectively, which gradually decreased/increased near to the control level due to release/uptake of water within a period of 25-30 min. ...200617301497
movement and bioaccumulation of chromium in an artificial freshwater ecosystem.in the present study, a small fresh water aquatic ecosystem was created into a small test tank to evaluate the movement and bioaccumulation of cr (vi) through water, sediment, a macrophyte hydrilla, small fish guppy, and few key organs of magur, clarias batrachus. the cr (vi) intoxication was imposed of as a single dose of 30 mg/l concentration for a wide range of exposure durations like 1, 7, 14 and 21 days. after 1 day of exposure the total cr (vi) load was very high in the water and sediment ...200717569292
levels of cadmium and lead in tissues of freshwater fish (clarias batrachus l.) and chicken in western up (india). 200717721732
estrogen-2/4-hydroxylase activity is stimulated during germinal vesicle breakdown induced by hcg, igf-1, gh and insulin in the catfish heteropneustes fossilis.estrogen-2/4-hydroxylase (eh) activity was measured radiometrically in ovaries of catfish injected with hcg intraperitoneally and in postvitellogenic follicles incubated with different concentrations of hcg, catfish (clarias batrachus) growth hormone (gh), bovine insulin or recombinant human insulin-like growth factor-i (rhigf-i). the change in enzyme activity was correlated with germinal vesicle breakdown (gvbd), an index of oocyte maturation. a single intraperitoneal injection of hcg (100 iu/f ...200817822703
differential affinity of natural haemagglutinin of macrobrachium rosenbergii towards vertebrate erythrocytes: effect of sex, size and moult stage on haemagglutination titre.lectins play important role in innate immunity of animals. the affinity of the natural haemagglutinin of the giant freshwater prawn macrobrachium rosenbergii towards vertebrate erythrocytes and its level with relation to sex, size and moult stages were studied. the strongest agglutinating titres in haemolymph of prawns were marked against guinea pig, chicken, clarias batrachus, and rabbit erythrocytes, and the weakest towards cattle, dog, horse and goat erythrocytes. a moderately agglutinating t ...200717907751
role of virulence plasmid of aeromonas hydrophila in the pathogenesis of ulcerative disease syndrome in clarias batrachus.pathogenic aeromonas hydrophila (strain vb21), a multiple-drug resistance strain contains a plasmid of about 21 kb. after curing of plasmid, the isolates became sensitive to antimicrobials, to which they were earlier resistant. the cured bacteria exhibited significant alterations in their surface structure, growth profile and virulence properties, and failed to cause ulcerative disease syndrome (uds) when injected into the indian catfish clarias batrachus. routine biochemical studies revealed th ...200718341217
catfish (clarias batrachus) serum lectin recognizes polyvalent tn [alpha-d-galpnac1-ser/thr], talpha [beta-d-galp-(1-->3)-alpha-d-galpnac1-ser/thr], and ii [beta-d-galp(1-->4)-beta-d-glcpnac1-] mammalian glycotopes.a new calcium dependent galnac/gal specific lectin was isolated from the serum of indian catfish, clarias batrachus and designated as c. batrachus lectin (cbl). it is a disulfide-linked homodecameric lectin of 74.65kda subunits and the oligomeric form is essential for its activity. binding specificity of cbl was investigated by enzyme-linked lectin-sorbent assay using a series of simple sugars, polysaccharides, and glycoproteins. galnac was more potent inhibitor than gal; and alpha glycosides of ...200818662810
association of cocaine- and amphetamine-regulated transcript and neuropeptide y in the forebrain and pituitary of the catfish, clarias batrachus: a double immunofluorescent labeling study.cocaine- and amphetamine-regulated transcript (cart) and neuropeptide y (npy) are involved in the regulation of food intake, body weight, pituitary hormones, and reproduction. while cart and npy occupy overlapping fields in the brain of mammals, little is known about the interaction between these peptide-containing systems in other vertebrates. we explored neuroanatomical associations between cart and npy in the olfactory system, forebrain and pituitary of the catfish, clarias batrachus, using d ...200818675898
chronic exposure to low concentration of arsenic is immunotoxic to fish: role of head kidney macrophages as biomarkers of arsenic toxicity to clarias batrachus.the present study was aimed at elucidating the effect of chronic low-level arsenic exposure on the head kidney (hk) of clarias batrachus and at determining the changes in head kidney macrophage (hkm) activity in response to arsenic exposure. chronic exposure (30 days) to arsenic (as(2)o(3), 0.50 microm) led to significant increase in arsenic content in the hk accompanied by reduction in both hkm number and head kidney somatic index (hksi). arsenic induced hk hypertrophy, reduction in melano-macr ...200919237206
microbiological quality of finfish and shellfish with special reference to shiga toxin-producing escherichia coli o157.the objective of this study was to determine the microbiological quality of fish and shellfish from kolkata, india, with special emphasis on e. coli o157. fresh and ice-preserved labeo rohita, catla catla, cirrhinus mrigala, oreochromis mossambica, heteropneustesfossilis, clarias batrachus, and penaeus monodon were examined for total heterotrophic bacteria and coliform loads and presence of e. coli and e. coli serotype o157 by culture method. while the total plate count of bacteria was within ac ...200819241559
clarias batrachus (linn.1758) population is lacking genetic diversity in india.genetic similarity and diversity of catfish clarias batrachus (linn.1758) populations collected from three regions of indian riverine system were examined using randomly amplified polymorphic dna-polymerase chain reaction (rapd-pcr). out of 22 random primers tested, six primers produced 462 rapd bands ranging from 105 to 128 polymorphic bands per primer in size between 100 and 1,200 bp. the polymorphic bands in these populations ranged from 26.5 to 30.5. polymorphic bands within populations rang ...201019360481
assessment of clarias batrachus as a source of acetylcholinesterase (ache) for the detection of insecticides.an inhibitive assay of insecticides using acetylcholinesterase (ache) from the local fish clarias batrachus is reported. ache was assayed according to the modified method of ellman. screening of insecticide and heavy metals showed that carbofuran and carbaryl strongly inhibited c. batrachus ache. the inhibition concentration (ic) ic50 values (and the 95% confidence interval) for both carbofuran and carbaryl inhibition on c. batrachus ache at 6.66 (5.97-7.52) and 130.00 (119.3-142.5) microg l(-1) ...200920112875
monoclonal antibodies to snakehead, channa striata immunoglobulins: detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs.snakehead channa striata is an important freshwater food fish in many southeast asian countries. three monoclonal antibodies (c9, c10 and d10) were developed against purified serum immunoglobulins of channa striata (cs-ig) and characterized. c9 and d10 mabs were specific to heavy chain, while c10 mab detected only unreduced cs-ig in western blotting. in competitive elisa, c9 and c10 mabs were specific to c. striata ig and showed no cross reactivity with serum ig of other fish species i.e. channa ...201021167285
establishment and characterization of a fin cell line from indian walking catfish, clarias batrachus (l.).a new cell line, indian catfish fin, derived from the fin tissue of indian walking catfish, clarias batrachus, was established and characterized. the cell line grew well in leibovitz's l-15 medium supplemented with 15% foetal bovine serum (fbs) and has been subcultured more than 110 times since its initiation in 2007. the cells were able to grow at a range of temperature from 28 to 37 °c with optimal growth at 28 °c. the cell line predominantly consists of fibroblast-like cells. the growth rate ...201121401643
lens proteome map and alpha-crystallin profile of the catfish rita rita.crystallins are a diverse group of proteins that constitute nearly 90% of the total soluble proteins of the vertebrate eye lens and these tightly packed crystallins are responsible for transparency of the lens. these proteins have been studied in different model and non-model species for understanding the modifications they undergo with ageing that lead to cataract, a disease of protein aggregation. in the present investigation, we studied the lens crystallin profile of the tropical freshwater c ...201121469600
development of est derived ssrs and snps as a genomic resource in indian catfish, clarias batrachus.clarias batrachus, an indian catfish species, is endemic to the indian subcontinent and potential cultivable species. the genomic resources in c. batrachus in the form of ests containing microsatellite repeats (est-ssr) and single nucleotide polymorphisms (snps) that are associated with the expressed genes from spleen were mined. from a total of 1,937 ests generated, 1,698 unique sequences were obtained, out of which 221 est-ssrs were identified and 54% could be functionally annotated by similar ...201122207175
lytic bacteriophages specific to flavobacterium columnare rescue catfish, clarias batrachus (linn.) from columnaris disease.this investigation was aimed to find out appropriate strategy against antibiotic resistant bacterial fish pathogen, f. columnare. this pathogen was found persistently associated with fishes causing columnaris disease and ensuing mass mortality in hatchery and culture system of sub - himalayan region. nine lytic f. columnare phages (fcp1 - fcp9) specific to its fifteen isolates were isolated from the water and bottom sediments of various geo-climatic regions of north india. the f. columnare phage ...201121882649
effect of ph and salinity on pathogenicity of flavobacterium columnare and myxobacterium sp. in indian cat fish, clarias batrachus (linn.) and heteropneustes fossilis (bloch.).flavobacterium columnare (fc) and myxobacterium sp. recorded persistently associated in fish hatchery and culture system of himalayan and sub - himalayan regions were found to be pathogenic. the ph and salinity played a significant role on the pathogenicity of these potent pathogens in case of clarias batrachus and heteropneustes fossilis. ld50 value of fc was 10(4.5) cfu in both the fishes and those of myxobacterium sp it was 10(6) cfu ml(-1) fish(-1). fish challenged with f. columnare and myxo ...201122319871
an enzymecytochemical study on the adrenal of the freshwater teleost, clarias batrachus (l.).the adrenal homologue of c. batrachus is distributed around the postcardinal vein in the pronephric head kidney. the cortical cells are round or oval in shape. they showed positive reaction for total lipid, glycogen and ascorbic acid. their intense delta5-3beta hsdh activity indicates their capacity for steroid biosynthesis. in addition, the cortical cells of c. batrachus exhibited strong g-6-pd, nadph diaphorase, nadh diaphorase, mao and weak sdh and ldh activity. the presence of mao suggests t ...1983217796
studies on the development of endocrine glands in fishes. ii. development of the pineal organ in clarias batrachus l. 20154455103
in vitro effects of divalent metal ions and metabolic modulators on purified glutamine synthetase from brain of teleostean fish.the purified brain glutamine synthetase of a teleostean fish, clarias batrachus has been examined under the influence of divalent metal ions activation and in vitro studies using different amino acids and metabolic modulators. all observations of the enzyme activity are based on transferase reaction at optimum temperature and ph. the enzyme exhibits an absolute requirement for mn2+ (most potent) mg2+ and co2+. the activity is markedly inhibited by leucine, asparagine, isoleucine, carbamyl phosph ...19951382667
changes in the hypothalamo-neurohypophysial complex of lead treated teleostean fish clarias batrachus (l.). 20133765780
angiotensin-converting enzyme in organs of air-breathing fish.angiotensin-converting enzyme (ace) was measured in tissue homogenates from the african lungfish and six species of air-breathing teleosts (heteropneustes fossilis, clarias batrachus, channa gachua, anabas testudineus, notopterus chitala, and monopterus cuchia) using a standard spectrophotometric assay. in most species, the highest levels of ace activity were found in the respiratory organs (gills and/or accessory respiratory organs). ace was also found in heart and kidney tissues from most spec ...20102830162
role of l-thyroxine, hydrocortisone and progesterone in regulation of blood volume in a fresh-water air-breathing fish, clarias batrachus (linn.).effect of hormones on the blood volume of clarias batrachus were ascertained and it was observed l-thyroxine and progesterone decrease, while hydrocortisone increases the total and relative blood volumes. the ratio of plasma to corpuscular volume is increased by the intraperitoneal injection of l-thyroxine, hydrocortisone and progesterone (higher dose only), thus indicating the regulation of blood volume under the complex control of homeostasis.200085575
effect of lead, zinc, mercury, and copper with and without estrogen on serum vitellogenin level in magur fish (clarias batrachus l.).injection(s) of lead, zinc, and mercuric acetate decreased the serum vitellogenin content in magur fish, while cupric acetate failed to cause any change in the vitellogenin level. estrogen injections on 7th, 8th and 9th d increased the serum vitellogenin level in normal and copper salt treated fish, but were totally ineffective in altering the reduced vitellogenin content in lead, zinc, and mercury salts treated fish. vitellogenin level almost restored to normal level at 6 week in lead, zinc, an ...20072099309
dichlorvos-induced histoarchitectural changes in the oocytes of a freshwater fish.the common edible air-breathing catfish clarias batrachus was exposed to lethal and sublethal concentrations of the organophosphorus insecticide dichlorvos. this produced significant cytoarchitectural changes in the oocytes, including pronounced vacuolation, degeneration and deformation. clumping of the cytoplasm and karyohypertrophy were also evident and rupture of the cell wall, with extrusion of the cytoplasm and the nuclei, was observed.20071793995
the response of nucleus preopticus neurosecretory cells to ovarian pressure in the teleost, clarias batrachus (linn.).the changes undergone by the neurosecretory cells of the nucleus preopticus (npo) were investigated histologically and immunocytochemically following pressure stimulation of the ovaries in the teleost, clarias batrachus. application of intraovarian pressure of 10 mm hg revealed marked hypertrophy of the cells, while pressures of 20 and 30 mm hg resulted in hypertrophy and exhaustion of the cells. no changes were observed when the treatment was preceded by transection of the spinal cord. the resu ...20123436513
an in situ study of the preoptico-neurohypophysial complex of the freshwater teleost clarias batrachus (l.). 20144185231
heavy metal pollutant induced changes in some biochemical parameters in the freshwater fish clarias batrachus l.the data on the effects of hg(ii), as(v) and pb(ii) on the biochemical parameters of the freshwater fish. clarias batrachus l., showed an increased protein content in the liver, kidney, stomach, intestine, testis and ovary, and a decreased content of it in the muscle as compared to control values. a decrease in dry weight and an increase in free amino acid and tissue permeability were recorded in all the organs after treatment with hg(ii), as(v) and pb(ii). in general, the organs were affected b ...20133751618
ameliorating er-stress attenuates aeromonas hydrophila-induced mitochondrial dysfunctioning and caspase mediated hkm apoptosis in clarias batrachus.endoplasmic reticulum (er)-stress and unfolding protein response (upr) has not been implied in aeromonas hydrophila-pathogenicity. we report increased expression of the er-stress markers: chop, bip and phospho-eif2α in a. hydrophila-infected headkidney macrophages (hkm) in clarias batrachus. pre-treatment with er-stress inhibitor, 4-pba alleviated er-stress and hkm apoptosis suggesting er-upr critical for the process. the er-ca(2+) released via inositol-triphosphate and ryanodine receptors induc ...201425059203
aeromonas hydrophila induced head kidney macrophage apoptosis in clarias batrachus involves the activation of calpain and is caspase-3 mediated.the mechanism of macrophage cytotoxicity induced by aeromonas hydrophila is yet unresolved. we observed a. hydrophila induces head kidney macrophage (hkm) apoptosis in clarias batrachus, as evident from hoechst 33342 and annexinv-propidium iodide staining and presence of oligonucleosomal dna ladder. initiation of apoptosis required the bacteria to be alive, be actively phagocytosed into hkm and was dependent on host proteins. elevated cytosolic calcium and consequent calpain activity that declin ...201222366184
autochthonous gut bacteria in two indian air-breathing fish, climbing perch (anabas testudineus) and walking catfish (clarias batrachus): mode of association, identification and enzyme producing ability.scanning electron microscopy (sem) was used to define the location of epithelium-associated bacteria in the gastrointestinal (gi) tract of two indian air-breathing fish, the climbing perch (anabas testudineus) and walking catfish (clarias batrachus). the sem examination revealed substantial numbers of rod shaped bacterial cells associated with the microvillus brush borders of enterocytes in proximal (pi) and distal regions (di) of the gi tract of both the fish species. ten (two each from the pi ...201526999956
monoclonal antibody to serum immunoglobulins of clarias batrachus and its application in immunoassays.serum immunoglobulins of clarias batrachus (cb-ig) were purified by affinity chromatography using bovine serum albumin as capture ligand. under reducing conditions in sds-page, cb-ig was composed of a heavy (h) chain (68.7 kda) and two light (l) chains (27.4 and 26.3 kda). purified cb-ig was used to produce a monoclonal antibody (mab) designated e4 mab that belonged to igg1 subclass. in western blotting, this mab showed binding to h chain of purified cb-ig and putative h chains in reduced sera o ...201223000018
isolation and immunohistochemical identification of flavobacterium psychrophilum from the tissue of catfish, clarias batrachus.this study deals with the identification of bacteria in paraffin embedded tissue of indian catfish, clarias batrachus experimentally infected with f. psychrophilum to ascertain it's precise role in the occurrence and state of carrier stage of coldwater disease in asymptomatic fishes. significant (p < 0.05) mortality was recorded in c. batrachus of this region due to persistent occurrence of creamish/yellowish with non smooth edged colonies of rod shaped flexible gram negative bacterium, f. psych ...201424665767
tapeworms (cestoda: caryophyllidea), parasites of clarias batrachus (pisces: siluriformes) in the indomalayan region.revision of monozoic cestodes (caryophyllidea) parasitic in commercially important walking catfish clarias batrachus (l.) in tropical asia (indomalayan zoogeographical region) was carried out, based on the evaluation of newly collected material from india, indonesia, and thailand, as well as a study of type specimens. instead of the 59 nominal taxa of 15 genera from 3 caryophyllidean families previously described, only 8 species of the lytocestidae are considered to be valid: bovienia indica (ni ...201121506765
seasonal infection of clarias batrachus (bloch) by lytocestus indicus moghe & parasitic effects on its haematology & histopathology. 19744464274
ascorbic acid, garlic extract and taurine alleviate cadmium-induced oxidative stress in freshwater catfish (clarias batrachus).an experiment was conducted to investigate bioaccumulation potential of cadmium (cd) and changes in oxidative stress indices in liver and kidney tissues from cd-exposed catfish (clarias batrachus) with or without simultaneous treatment of water with ascorbic acid, garlic extract or taurine. c. batrachus (n=324) with average length of 20+/-4 cm and weight of 86+/-5 g were used for the present investigation. fishes were divided into nine groups (i to ix) each comprising 36 fishes. the fishes of gr ...200919552941
vascular corrosion replicas of chemo-baroreceptors in fish: the carotid labyrinth in ictaluridae and clariidae.scanning electron microscopy of vascular corrosion replicas and light microscopy revealed a pair of highly vascularized tissues, the carotid labyrinths, in the dorsal head region of the channel catfish, ictalurus punctatus, the black bullhead, i. melas, and the walking catfish, clarias batrachus. the labyrinth consists of a myriad of arterioles that arise from the common carotid artery immediately distal to the origin of the common carotid from the efferent branchial (epibranchial) artery of the ...19817273113
cell volume affects glycogen phosphorylase activity in fish hepatocytes.the activity of glycogen phosphorylase (gpase) in the active a-form (gpase a) is dependent on the hydration state of hepatocytes. we establish that gpase a catalysis in catfish (ameiurus nebulosus) hepatocytes is a function of medium osmolarity and that a linear relationship exists between gpase a activity and osmolarity between 254 mosmol l(-1) and 478 mosmol l(-1). exposure of isolated hepatocytes to hyperosmotic media increases enzyme activity up to 7-fold, indicative of covalent phosphorylat ...200312898165
biochemical composition of some small indigenous fresh water fishes from the river mouri, khulna, bangladesh.biochemical composition of seven small indigenous fresh water fishes namely magur (clarias batrachus), shingi (heteropneustes fossilis), koi (anabas testudineus), foli (notopterus notopterus), royna (nandas nandas), taki (channa punctatus) and tangra (mystus vittatus) from the mouri river khulna, bangladesh was studied in order to evaluate their nutritional values. the mean value of protein, fat, moisture and ash content was found as 14.87 +/- 0.63, 7.90 +/- 1.91, 73.49 +/- 0.69 and 3.74 +/- 0.4 ...200719069978
hypophysiotropic neurons in the hypothalamus of the catfish clarias batrachus: a cobaltous lysine and hrp study.seven hypothalamic nuclei and several isolated perikarya that send projections to the pituitary gland were identified following administration of cobaltous lysine or horseradish peroxidase (hrp) to severed hypophysial stalks of previously hypophysectomized catfish, clarias batrachus. retrogradely labelled neurons were identified in the nucleus preopticus periventricularis, suprachiasmatic nucleus, paraventricular and supraoptic divisions of the magnocellular nucleus preopticus (npo) and nucleus ...19938324622
hypothalamic innervation of the pituitary in the catfish, clarias batrachus (l.): a retrograde horseradish peroxidase study.intrahypophysial administration of horseradish peroxidase (hrp) resulted in extensive labelling of the cells of nucleus preopticus and nucleus lateralis tuberis. besides, isolated retrogradely labelled neurons were observed in the nucleus preopticus periventricularis in the preoptic area, nucleus of the horizontal commissure, nucleus hypothalamicus ventralis in the rostral tuberal area, and nucleus arcuatus hypothalamicus in the caudal tuberal area. a few labelled cells were also observed in the ...19892616047
production of fertile sperm from in vitro propagating enriched spermatogonial stem cells of farmed catfish, clarias batrachus.spermatogenesis is a highly co-ordinated and complex process. in vitro propagation of spermatogonial stem cells (sscs) could provide an avenue in which to undertake in vivo studies of spermatogenesis. very little information is known about the ssc biology of teleosts. in this study, collagenase-treated testicular cells of farmed catfish (clarias batrachus, popularly known as magur) were purified by ficoll gradient centrifugation followed by magnetic activated cell sorting using thy1.2 (cd90.2) a ...201627417669
isolation of heat-tolerant myoglobin from asian swamp eel monopterus albus.myoglobin from asian swamp eel monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. the purified mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by maldi-tof-ms to be 15,525.18 da. using isoelectric focusing technique, the purified mb showed two derivatives with pi of 6.40 and 7.12. six peptide fragments of this protein identified by lc-ms/ms were homologous to mbs of sea raven hemitripterus ...201222538454
physical modification of palm kernel meal improved available carbohydrate, physicochemical properties and in vitro digestibility in economic freshwater fish.unavailable carbohydrates are an important limiting factor for utilization of palm kernel meal (pkm) as aquafeed ingredients. the aim of this study was to improve available carbohydrate from pkm. different physical modifications including water soaking, microwave irradiation, gamma irradiation and electron beam, were investigated in relation to chemical composition, physicochemical properties and in vitro carbohydrate digestibility using digestive enzymes from economic freshwater fish.201323881536
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