| treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. | a highly motile, medium-size, saccharolytic spirochete was isolated from an advanced human periodontal lesion in medium omiz-pat supplemented with 1% human serum. the growth of this organism is dependent on either glucose, maltose, starch, or glycogen. the cells contain six endoflagella, three per pole, which overlap in the central region of the cell body. on the basis of its cell morphology and enzyme activities, as well as its sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein a ... | 1997 | 9226916 |
| phylogenetic analysis of cultivable oral treponemes from the smibert collection. | dr. robert smibert from the virginia polytechnic institute, usa, isolated and collected over 200 strains of oral treponemes over a 20-year period. dr. smibert, dr. w.e.c. moore and dr. l.v. moore separated these isolates and reference strains into different groups on the basis of cellular fatty acid analysis. in this study, the 16s rrna genes were sequenced for 47 strains that were representative of these groups. five distinct species were identified on the basis of 16s rrna sequence comparisons ... | 1998 | 9734025 |
| identification of seven treponema species in health- and disease-associated dental plaque by nested pcr. | species-specific nested pcr was used to detect treponema amylovorum, treponema denticola, treponema maltophilum, treponema medium, treponema pectinovorum, treponema socranskii, and treponema vincentii in dental plaque. subjects with periodontitis harbored all species, but t. pectinovorum and t. vincentii were not found in plaque from disease-free subjects. | 1999 | 9986879 |
| pcr-based identification of treponema maltophilum, t amylovorum, t medium, and t lecithinolyticum in primary root canal infections. | molecular genetic methods have significantly contributed to the knowledge about the microbiota associated with infected root canals. albeit spirochetes have been commonly observed in primary root canal infections, only recently they have been identified. the purpose of the present study was to investigate the occurrence of four treponemes-treponema maltophilum, treponema lecithinolyticum, treponema amylovorum, and treponema medium-in cases of primary endodontic infections associated with differe ... | 2003 | 12798152 |
| treponema species associated with abscesses of endodontic origin. | spirochetes have been frequently observed in abscesses of endodontic origin, but they have rarely been identified. this study sought to investigate the prevalence of eight oral treponemes in acute periradicular abscesses using a species-specific nested polymerase chain reaction assay. purulent exudate was collected by aspiration from 19 cases diagnosed as acute periradicular abscesses and dna extracted from the samples was initially amplified using universal 16s rdna primers. a second round of a ... | 2004 | 15327648 |
| analysis of the complement sensitivity of oral treponemes and the potential influence of fh binding, fh cleavage and dentilisin activity on the pathogenesis of periodontal disease. | treponema denticola, a periopathogen, evades complement-mediated killing by binding the negative complement regulatory protein factor h (fh) to its surface via the fhbb protein. paradoxically, bound fh is cleaved by t. denticola's dentilisin protease, a process hypothesized to trigger localized dysregulation of complement activation in periodontal pockets. the ability of other oral treponemes to evade complement-mediated killing and bind and cleave fh has not been assessed. in this report, we de ... | 2014 | 24815960 |
| effect of cigarette smoking on subgingival bacteria in healthy subjects and patients with chronic periodontitis. | cigarette smoking is known to increase the risk of periodontal destruction and developing chronic periodontitis (cp). it is also reported to affect the subgingival bacterial profile among cp patients. however, studies on the effect of smoking on the bacterial profile among healthy subjects are still limited. therefore, the aim of this study was to investigate the impact of smoking on the subgingival bacterial profile in both healthy adults and cp patients. | 2017 | 28327165 |