paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. | during a search for xylan-degrading micro-organisms, a sporulating bacterium was recovered from xylan-containing agar plates exposed to air in a research laboratory (salamanca university, spain). the airborne isolate (designated strain xil14t) was identified by 16s rrna gene sequencing as representing a paenibacillus species most closely related to paenibacillus illinoisensis jcm 9907t (99.3 % sequence similarity) and paenibacillus pabuli dsm 3036t (98 % sequence similarity). phenotypic, chemota ... | 2005 | 15653909 |
paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the ebro river delta. | a gram-positive, endospore-forming, xylanase-producing bacterium isolated from a rice field was studied taxonomically. the strain grows at 10-40 degrees c and in the presence of lysozyme or 5 % (w/v) nacl. chemotaxonomic analysis revealed that mk-7 was the predominant menaquinone of the isolated strain, while the major fatty acid was anteiso-c(15 : 0). comparison of 16s rrna gene sequences showed that strain bp-23(t) fell within the radiation of the cluster comprising paenibacillus species. the ... | 2005 | 15774688 |
chitinases produced by paenibacillus illinoisensis and bacillus thuringiensis subsp. pakistani degrade nod factor from bradyrhizobium japonicum. | chitinases are enzymes that hydrolyze internal beta-1,4-n-acetyl-d-glucosamine linkages of chitin. since the backbone of nod factors is a chitin oligomer, we investigated whether chitinases produced by soil bacteria paenibacillus illinoisensis kja-424 and bacillus thuringiensis subsp. pakistani hd 395 are able to degrade nod factor produced by bradyrhizobium japonicum, a phenomenon that could disrupt b. japonicum-soybean signaling and nodule establishment when chitinases are present. purified no ... | 2008 | 16904303 |
paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and paenibacillus urinalis sp. nov., isolated from human urine. | gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403(t)) and a human cerebrospinal fluid sample (strain 4401170(t)). based on genotypic characteristics, these strains belonged to the genus paenibacillus and were closely related. phylogenetic analysis based on 16s rrna gene sequence comparison showed that they clustered with paenibacillus massiliensis 2301065(t) (95.9 and 94.3 % 16s rrna gene sequence similarity, respectively, for strains 5402403(t) and 440117 ... | 2008 | 18319478 |
paenibacillus taichungensis sp. nov., from soil in taiwan. | among a large collection of taiwanese soil isolates, a novel gram-variable, rod-shaped, motile, endospore-forming bacterial strain, strain v10537(t), was subjected to a polyphasic study including 16s rrna and gyrb gene sequence analysis, dna-dna hybridization experiments, cell wall peptidoglycan type, cellular fatty acid composition analysis and comparative phenotypic characterization. 16s rrna gene sequence analysis indicated that the organism belonged to the genus paenibacillus. strain v10537( ... | 2008 | 18984707 |
paenibacillus panacisoli sp. nov., a xylanolytic bacterium isolated from soil in a ginseng field in south korea. | a gram-positive, facultatively anaerobic, motile, spore-forming bacterium, designated gsoil 1411t, was isolated from soil of a ginseng field in pocheon province (south korea) and was characterized using a polyphasic approach. comparative analysis of 16s rrna gene sequences revealed that strain gsoil 1411t belongs to the family paenibacillaceae, with closest sequence similarity to the type strains of paenibacillus xylanilyticus (95.7%), paenibacillus illinoisensis (95.2%) and paenibacillus pabuli ... | 2006 | 17082410 |
isolation and 16s dna characterization of soil microorganisms from tropical soils capable of utilizing the herbicides hexazinone and tebuthiuron. | six non-fermentative bacteria were isolated from colombian (south america) and hawaiian (usa) soils after enrichment with minimal medium supplemented with two herbicides, hexazinone (hex) and tebuthiuron (teb). microscopic examination and physiological tests were followed by partial 16s dna sequence analysis, using the first 527 bp of the 16s rrna gene for bacterial identification. the isolated microorganisms (and in brackets, the herbicide that each degraded) were identified as: from colombia. ... | 2003 | 14649709 |
purification and properties of extracellular dextranase from a bacillus sp. | bacterial strains in the genus bacillus were isolated from natural soil samples and screened for production of extracellular dextranases (e.c.3.2.1.11). one strain, determined by 16srna analysis as paenibacillus illinoisensis exhibiting stable dextranase activity, was chosen for further analysis, and the dextranase from it was purified 733-fold using salt and peg precipitations, two-phase extraction and deae-sepharose chromatography with a total yield of 19%. the purified enzyme had three isofor ... | 2003 | 14581071 |
isolation of paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents. | a bacterium that secreted cyclodextrin glucanotransferase (cgtase) in a medium overlaid with n-hexane was isolated and identified as paenibacillus illinoisensis strain st-12 k. the cgtase of the strain was purified from the culture supernatant. the molecular mass was 70 kda. the enzyme was stable at ph 6 to 10 and active at ph 5.0 to 8.0. the optimum temperature at ph 7.0 was 65 degrees c in the presence of 5 mm cacl2. the enzyme produced mainly beta-cyclodextrin. the total yield of alpha-, beta ... | 2003 | 12728995 |
emended description of paenibacillus amylolyticus and description of paenibacillus illinoisensis sp. nov. and paenibacillus chibensis sp. nov. | the taxonomic position of unidentified group 6 of bacillus circulans as described by nakamura and swezey (l.k. nakamura and j. swezey, int. j. syst. bacteriol. 33:46-52, 1983) was determined, and the taxonomy of paenibacillus amylolyticus was reexamined. the results of pcr amplification of a 16s rrna gene fragment with a specific primer and comparative analysis of 16s rrna gene sequences warranted placing the two taxa in the genus paenibacillus. the levels of dna reassociation among the strains ... | 1997 | 9103613 |
isolation and characterization of methane utilizing bacteria from wetland paddy ecosystem. | methylotrophic bacteria which are known to utilize c1 compounds including methane. research during past few decades increased the interest in finding out novel genera of methane degrading bacteria to efficiently utilize methane to decrease global warming effect. moreover, evaluation of certain known plant growth promoting strains for their methane degrading potential may open up a new direction for multiple utility of such cultures. in this study, efficient methylotrophic cultures were isolated ... | 2014 | 24469547 |
paenibacillus kyungheensis sp. nov., isolated from flowers of magnolia. | a gram-staining-positive, catalase-positive, oxidase-negative, facultatively anaerobic, rod-shaped bacterium designated strain dcy88t, was isolated from flowers of magnolia. phylogenetic analysis based on 16s rrna gene sequence comparison revealed that the strain formed a distinct lineage within the genus paenibacillus that was closely related to paenibacillus hordei rh-n24t (97.8 %). the other most closely related species were paenibacillus illinoisensis nrrl nrs-1356t (94.3 %), paenibacillus h ... | 2015 | 26268929 |
β-cyclodextrin production by the cyclodextrin glucanotransferase from paenibacillus illinoisensis zy-08: cloning, purification, and properties. | the gene encoding the cyclodextrin glucanotransferase (cgtase, ec2.4.1.19) of paenibacillus illinoisensis was isolated, cloned, sequenced and expressed in escherichia coli. sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34-residues. the deduced amino acid sequence of the cgtase from p. illinoisensis zy-08 exhibited highest identity (99 %) to the cgtase sequence from bacillus licheniformis (p14014). the four consensus regions of carbohydrate ... | 2012 | 23264152 |
paenibacillus hordei sp. nov., isolated from naked barley in korea. | a gram-staining positive, facultative aerobic bacterium, designated strain rh-n24(t), was isolated from naked barley in south korea. cells of the isolate were observed to be motile rods by means of peritrichous flagella and showed catalase-positive and oxidase-negative reactions. growth of strain rh-n24(t) was observed at 4-40 °c (optimum: 35-37 °c) and at ph 5.0-9.0 (optimum: ph 6.0-7.0). chemotaxonomic data (major isoprenoid quinone: mk-7; dna g + c content: 53.5 mol %; cell wall type: a1γ-mes ... | 2013 | 22806133 |