pantoea punctata sp. nov., pantoea citrea sp. nov., and pantoea terrea sp. nov. isolated from fruit and soil samples.a total of 37 bacterial strains with the general characteristics of the family enterobacteriaceae were isolated from fruit and soil samples in japan as producers of 2,5-diketo-d-gluconic acid from d-glucose. these organisms were phenotypically most closely related to the genus pantoea (f. gavini, j. mergaert, a. beji, c. mielearek, d. izard, k. kersters, and j. de ley, int. j. syst. bacteriol. 39:337-345, 1989) and were divided into three phenotypic groups. we selected nine representative strain ...19921581180
mechanisms of biocontrol by pantoea dispersa of sugar cane leaf scald disease caused by xanthomonas albilineans.albicidins, a family of phytotoxins and antibiotics produced by xanthomonas albilineans, are important in sugar cane leaf scald disease development. the albicidin detoxifying bacterium pantoea dispersa (syn. erwinia herbicola) sb1403 provides very effective biocontrol against leaf scald disease in highly susceptible sugar cane cultivars. the p. dispersa gene (albd) for enzymatic detoxification of albicidin has recently been cloned and sequenced. to test the role of albicidin detoxification in bi ...19979134719
characterization of pantoea dispersa uq68j: producer of a highly efficient sucrose isomerase for isomaltulose biosynthesis.isolation, identification and characterization of a highly efficient isomaltulose producer.200415186446
characterization of the highly efficient sucrose isomerase from pantoea dispersa uq68j and cloning of the sucrose isomerase gene.sucrose isomerase (si) genes from pantoea dispersa uq68j, klebsiella planticola uq14s, and erwinia rhapontici wac2928 were cloned and expressed in escherichia coli. the predicted products of the uq14s and wac2928 genes were similar to known sis. the uq68j si differed substantially, and it showed the highest isomaltulose-producing efficiency in e. coli cells. the purified recombinant wac2928 si was unstable, whereas purified uq68j and uq14s sis were very stable. uq68j si activity was optimal at p ...200515746363
reliability of diagnostic techniques for erwinia amylovora, the causative agent of fire blight disease.a total of 20 putative strains of erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody for pta-elisa (plate-trapped antigen-enzyme linked immunosorbent assay) at a concentration corresponding to a620 = 0.1, while at a620 readings of 0.01 and 0.001 the results were nega ...200516295660
emended description of the genus pantoea, description of four species from human clinical samples, pantoea septica sp. nov., pantoea eucrina sp. nov., pantoea brenneri sp. nov. and pantoea conspicua sp. nov., and transfer of pectobacterium cypripedii (hori 1911) brenner et al. 1973 emend. hauben et al. 1998 to the genus as pantoea cypripedii comb. nov.bacterial strains belonging to dna hybridization groups (hg) ii, iv and v, in the erwinia herbicola-enterobacter agglomerans complex, of brenner et al. [int j syst bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus pantoea, but have never been formally described and classified. additionally, it has been shown in several studies that pectobacterium cypripedii is more closely related to species of pantoea than to those of pectobacterium. in this study, the phylogenetic po ...201019946052
bacterial species associated with sound and botrytis-infected grapes from a greek vineyard.grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ...201121315469
analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested pcr-denaturing gradient gel electrophoresis and the plate count method.the diversity of bacteria associated with the fermentation of inyu, also known as black soy sauce, was studied through the nested pcr-denaturing gradient gel electrophoresis (dgge) of samples collected from the fermentation stages of the inyu production process. the dgge profiles targeted the bacterial 16s rdna and revealed the presence of citrobacter farmeri, enterobacter cloacae, enterobacter hormaechei, enterococcus faecium, klebsiella pneumoniae, pantoea agglomerans, salmonella enterica, ser ...201323200659
formulation of medium constituents by multiresponse analysis of central composite design to enhance chitinase production in pantoea the present study, a high chitinase producing strain pantoea dispersa was isolated from the sea dumps at bhavnagar, india. chitin, urea, cacl2 and mgso4 x 7h2o were variables used in central composite design for chitinase production. chitinase, biomass and ph were the responses used in different models to evaluate individually fit ones. quadratic model was found to be fit for chitinase response whereas in the case of biomass and ph, linear model was found to be fit without the effect of other ...200415587121
isolation and identification of marine chitinolytic bacteria and their potential in antifungal biocontrol.chitinolytic marine bacterial strains (30) were isolated from the sea dumps at bhavnagar, india. they were screened as chitinase producers on the basis of zone of clearance on chitin agar plates incorporated with calcofluor white m2r for the better resolution. out of these, three strains namely, pseudomonas sp., pantoea dispersa and enterobacter amnigenus showed high chitinase production. they were also found to produce proteases and therefore have a good potential for use as antifungal biocontr ...200415339036
isolation of a pantoea dispersa-like strain fron a 71-year-old woman with acute myeloid leukemia and multiple myeloma. 200312608369
high affinity binding of albicidin phytotoxins by the alba protein from klebsiella oxytoca.albicidins are a family of phytotoxins and antibiotics which play an important role in the pathogenesis of sugarcane leaf scald disease. the alba gene from klebsiella oxytoca encodes a protein which inactivates albicidin by heat-reversible binding. albicidin ligand binding to a recombinant alba protein, purified by means of a glutathione s-transferase gene fusion system, is an almost instant and saturable reaction. kinetic and stoichiometric analysis of the binding reaction indicated the presenc ...19989493391
the gene for albicidin detoxification from pantoea dispersa encodes an esterase and attenuates pathogenicity of xanthomonas albilineans to sugarcane.albicidin phytotoxins are pathogenicity factors in a devastating disease of sugarcane known as leaf scald, caused by xanthomonas albilineans. a gene (albd) from pantoea dispersa has been cloned and sequenced and been shown to code for a peptide of 235 amino acids that detoxifies albicidin. the gene shows no significant homology at the dna or protein level to any known sequence, but the gene product contains a gxsxg motif that is conserved in serine hydrolases. the albd protein, purified to homog ...19979275238
fungal and bacterial diversity isolated from aquilaria malaccensis tree and soil, induces agarospirol formation within 3 months after artificial infection.aquilaria malaccensis lam, commonly known as agarwood, is a highly valuable species used in production of agar oil from its infected wood, which is utilized in pharmaceutical and perfumery industry. agar oil formation in agarwood takes years through the natural process which is induced by natural or artificial injury or microbial infection. the role of soil fungi and bacteria in artificial induction is still an unexplored area. in the present study, we isolated the fungal and bacterial community ...201728747900
indoleacetic acid production and plant growth promoting potential of bacterial endophytes isolated from rice (oryza sativa l.) seeds.bacterial endophytes from the phyllosphere and rhizosphere have been used to produce bioactive metabolites and to promote plant growth. however, little is known about the endophytes residing in seeds. this study aimed to isolate and identify seed-borne bacterial endophytes from rice and elucidate their potential for phytohormone production and growth enhancement. the isolated endophytes included micrococcus yunnanensis rwl-2, micrococcus luteus rwl-3, enterobacter soli rwl-4, leclercia adecarbox ...201728605980
the isolation and identification of pantoea dispersa strain jfs as a non-pathogenic surrogate for salmonella typhimurium phage type 42 in flour.salmonella is a common pathogen which has been the cause of foodborne illness outbreaks implicating a variety of commodities, including low-moisture foods such as flour. salmonella costs more than any other pathogen in the united states in terms of health care expenses and time of lost work. heat treatment can be used to reduce salmonella and other pathogens in flour to safe levels. however, in low-moisture foods, process times must be increased to achieve adequate lethality, possibly resulting ...201626686596
pantoea dispersa: an unusual cause of neonatal sepsis.neonatal septicemia is the most important cause of neonatal mortality. a wide variety of bacteria both aerobic and anaerobic can cause neonatal sepsis. genus pantoea is a member of enterobacteriaceae family that inhabits plants, soil and water and rarely causes human infections, however, pantoea dispersa has not been reported as a causative organism for neonatal sepsis. we hereby report two neonates with early onset sepsis caused by pantoea dispersa. early detection and appropriate antibiotic th ...201324120830
bacteria associated with piezodorus guildinii (hemiptera: pentatomidae), with special reference to those transmitted by feeding.the redbanded stink bug, piezodorus guildinii (westwood) (hemiptera: heteroptera: pentatomidae), is a rapidly growing pest damaging southern us agriculture. pentatomid stink bugs are known to vector bacterial, fungal, and viral plant diseases. however, bacteria associated with redbanded stink bugs and their vector potential have not yet been assessed. in this study, we 1) cultured and identified bacteria transmitted by feeding of redbanded stink bug and 2) described bacteria from guts of redband ...201728025221
16s ribosomal dna sequence-based identification of bacteria in laboratory rodents: a practical approach in laboratory animal bacteriology diagnostics.correct identification of bacteria is crucial for the management of rodent colonies. some bacteria are difficult to identify phenotypically outside reference laboratories. in this study, we evaluated the utility of 16s ribosomal dna (rdna) sequencing as a means of identifying a collection of 30 isolates of rodent origin which are conventionally difficult to identify. sequence analysis of the first approximate 720 to 880 bp of the 5'- end of 16s rdna identified 25 isolates (83.33%) with ≥ 99% sim ...201424876090
pantoea beijingensis sp. nov., isolated from the fruiting body of pleurotus eryngii.four gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from a fruiting body of the edible mushroom pleurotus eryngii showing symptoms of soft rot disease in beijing, china. partial 16s rrna gene sequencing, together with partial rpob sequencing, placed these isolates in the genus pantoea. multilocus sequence analysis based on the partial sequences of gyrb, rpob, infb and atpd revealed pantoea dispersa and pantoea gaviniae as their closest phylogenetic relatives and ...201324013967
indole-3-acetic acid biosynthetic pathway and aromatic amino acid aminotransferase activities in pantoea dispersa strain gpk.this investigation deals with the production of iaa by a bacterial isolate pantoea dispersa strain gpk (pdg) identified by 16s rrna gene sequence analysis. hplc and mass spectral analysis of metabolites from bacterial spent medium revealed that, iaa production by pdg is trp-dependent and follows indole-3-pyruvic acid (ipya) pathway. substrate specificity study of aromatic amino acid aminotransferase (aat) showed high activities, only when tryptophan (trp) and α-ketoglutarate (α-kg) were used as ...201323448265
identification of the corn pathogen pantoea stewartii by mass spectrometry of whole-cell extracts and its detection with novel pcr primers.pantoea stewartii subsp. stewartii is the causative agent of stewart's wilt, a bacterial disease transmitted by the corn flea beetle mainly to sweet corn (zea mays). in many countries, it is classified as a quarantine organism and must be differentiated from other yellow enteric bacteria frequently occurring with corn. we have created novel primers from the psts-glms region of p. stewartii for use in conventional pcr (cpcr) and quantitative pcr (qpcr). to facilitate rapid diagnosis, we applied m ...201020656863
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
bioconversion of lutein to form aroma compounds by pantoea investigate the conversion of lutein, a carotenoid, to aroma compounds by pantoea dispersa y08, a lutein-degrading bacterium isolated from marigold flower residue. bioconversion conditions, including substrate concentration, applied co-solvent and reaction time, were optimized.201526067662
pantoea rodasii sp. nov., pantoea rwandensis sp. nov. and pantoea wallisii sp. nov., three novel pantoea species isolated from eucalyptus.several gram-negative, facultatively anaerobic bacterial isolates were obtained from eucalyptus seedlings showing symptoms of bacterial blight and dieback in colombia, rwanda and south africa. partial 16s rrna gene sequencing, together with partial gyrb sequencing, placed the isolates in the genus pantoea and indicated they constituted three novel species. multilocus sequence analysis (mlsa) based on partial sequences of gyrb, rpob, infb and atpd revealed p. dispersa, p. eucrina and p. cypripedi ...201121841003
the albicidin resistance factor albd is a serine endopeptidase that hydrolyzes unusual oligoaromatic-type peptides.the para-aminobenzoic acid-containing peptide albicidin is a pathogenicity factor synthesized by xanthomonas albilineans in infections of sugar cane. albicidin is a nanomolar inhibitor of the bacterial dna gyrase with a strong activity against various gram-negative bacteria. the bacterium pantoea dispersa expresses the hydrolase albd, conferring natural resistance against albicidin. we show that albd is a novel type of endopeptidase that catalyzes the cleavage of albicidin at a peptide backbone ...201526057615
pantoea dispersa bacteremia caused by central line-associated bloodstream infection. 201425179511
combined effects of clay immobilized azospirillum brasilense and pantoea dispersa and organic olive residue on plant performance and soil properties in the revegetation of a semiarid area.the reestablishment of autochthonous shrubs species is an essential strategy for recovering degraded soils under semiarid mediterranean areas. a field experiment was carried out to assess the effectiveness of an immobilized microbial inoculant (azospirillum brasilense and pantoea dispersa) and the addition of organic olive residue (alperujo), for plant growth promotion of cistus albidus l. and enhancement of soil properties. sixteen months after planting, the microbial inoculant and organic resi ...201423895777
comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis.detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ...201020526836
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