| immunocytochemical detection of dna and rna in endosymbiont-bearing trypanosomatids. | research about the kinetoplast of trypanosomatids has yielded valuable information about the organization of extranuclear structure. however, the ultrastructural localization of nucleic acids within these protozoa remains uncertain. we have applied cytochemical and immunocytochemical approaches to precisely identify dna and rna in lower endosymbiont-bearing trypanosomatids. using the terminal deoxynucleotidyl transferase (tdt) immunogold technique, we showed that nuclear dna is seen associated w ... | 2003 | 12694905 |
| differential expression of proteolytic enzymes in endosymbiont-harboring crithidia species. | crithidia oncopelti, crithidia deanei and crithidia desouzai are flagellates of the trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. gelatin-sds-page analysis was used to characterize cell-associated and extracellular proteinases in these organisms. our survey indicates that the proteolytic profiles of c. deanei and c. desouzai are identical; that c. oncopelti displays a distinct zymogram; and that species naturally lacking endosymbionts have a more complex e ... | 2001 | 11506910 |
| purification and partial characterization of trypanosoma cruzi triosephosphate isomerase. | the enzyme triosephosphate isomerase (tpi, ec 5.3.1.1) was purified from extracts of epimastigote forms of trypanosoma cruzi. the purification steps included: hydrophobic interaction chromatography on phenyl-sepharose, cm-sepharose, and high performance liquid gel filtration chromatography. the cm-sepharose material contained two bands (27 and 25 kda) with similar isoelectric points (pi 9.3-9.5) which could be separated by gel filtration in high performance liquid chromatography. polyclonal anti ... | 1998 | 9698898 |
| endosymbiosis in protozoa of the trypanosomatidae family. | a small number of trypanosomatids present bacterium endosymbionts in the cytoplasm, which divide synchronously with the host cell. crithidia oncopleti, crithidia deanei. crithidia desouzai, blastocrithidia culicis and herpetomonas roitmani are the best characterized species. the endosymbiont is surrounded by two membranes separated from each other by an electron-lucent space. the presence of the endosymbiont led to the appearance of morphological changes which include the lack of the paraflagell ... | 1999 | 10220875 |