| novel glycine containing glucolipids from the alkane using bacterium alcanivorax borkumensis. | the polar lipids from the hydrocarbon using and biosurfactant-producing bacterium alcanivorax borkumensis were isolated and identified by mass spectrometry (ms) and nuclear magnetic resonance (nmr) spectroscopy. the biosurfactant produced by this species is an anionic glucose lipid with a tetrameric oxyacyl side chain. the glycolipids extracted from the cell wall consist of this biosurfactant n-terminally esterified with glycine. ten different derivatives of this lipid type were identified and t ... | 1998 | 9714737 |
| alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. | during screening for biosurfactant-producing, n-alkane-degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea water/sediment samples collected near the isle of borkum (north sea), using mihagol-s (c14,15-n-alkanes) as principal carbon source. these gram-negative, aerobic, rod-shaped bacteria use a limited number of organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and pyruvate and its methyl ether. dur ... | 1998 | 9731272 |
| effect of novel biosurfactants on biodegradation of polychlorinated biphenyls by pure and mixed bacterial cultures. | a study was conducted to determine the potential positive effect of novel biosurfactants on the enhancement of aroclor 1248 metabolization in both in vitro and in situ experiments. among two lipopeptides tested the highest activity was found in experiments with a hydrolytically opened form of lichenysin a. lichenysin a itself did not enhance the degradation activity of chosen microorganism-degraders and in most cases inhibited their pcb mineralization rates. glucolipid surfactant from marine bac ... | 1999 | 10423745 |
| petroleum biodegradation in marine environments. | petroleum-based products are the major source of energy for industry and daily life. petroleum is also the raw material for many chemical products such as plastics, paints, and cosmetics. the transport of petroleum across the world is frequent, and the amounts of petroleum stocks in developed countries are enormous. consequently, the potential for oil spills is significant, and research on the fate of petroleum in a marine environment is important to evaluate the environmental threat of oil spil ... | 1999 | 10941786 |
| biodegradation of n-alkylcycloalkanes and n-alkylbenzenes via new pathways in alcanivorax sp. strain mbic 4326. | the degradation of long-chain n-alkylbenzenes and n-alkylcyclohexanes by alcanivorax sp. strain mbic 4326 was investigated. the alkyl side chain of these compounds was mainly processed by beta-oxidation. in the degradation of n-alkylcyclohexanes, cyclohexanecarboxylic acid was formed as an intermediate. this compound was further transformed to benzoic acid via 1-cyclohexene-1-carboxylic acid. | 2001 | 11282659 |
| molecular detection of marine bacterial populations on beaches contaminated by the nakhodka tanker oil-spill accident. | in january 1997, the tanker nakhodka sank in the japan sea, and more than 5000 tons of heavy oil leaked. the released oil contaminated more than 500 km of the coastline, and some still remained even by june 1999. to investigate the long-term influence of the nakhodka oil spill on marine bacterial populations, sea water and residual oil were sampled from the oil-contaminated zones 10, 18, 22 and 29 months after the accident, and the bacterial populations in these samples were analysed by denaturi ... | 2001 | 11359510 |
| development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading alcanivorax strains. | the genus alcanivorax comprises diverse hydrocarbon-degrading marine bacteria. novel 16s rrna-targeted oligonucleotide dna probes (alv735 and alv735-b) were developed to quantify two subgroups of the alcanivorax/fundibacter group by fluorescence in situ hybridization (fish), and the conditions for the single-mismatch discrimination of the probes were optimized. the specificity of the probes was improved further using a singly mismatched oligonucleotide as a competitor. the growth of alcanivorax ... | 2001 | 11472502 |
| [typing and evaluation of hydrocarbon-degrading activity of a bacterial strain isolated from the bahía blanca estuary, argentina]. | during screening for oil-degrading bacteria in sediments of bahía blanca estuary, a bacterial strain was isolated which uses a limited number of organic compounds, especially lineal hydrocarbons. the goals of this study were to characterize the strain and to evaluate its activity on crude oil. based on phenotypic characteristics and the analysis of 16s rdna sequences, it was determined that the strain is strongly related to alcanivorax borkumensis. the strain was cultured, under quasicontinuous ... | 2001 | 11594004 |
| functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria. | we have cloned homologs of the pseudomonas putida gpo1 alkane hydroxylase from pseudomonas aeruginosa pao1, pseudomonas fluorescens cha0, alcanivorax borkumensis ap1, mycobacterium tuberculosis h37rv, and prauserella rugosa nrrl b-2295. sequence comparisons show that the level of protein sequence identity between the homologs is as low as 35%, and that the pseudomonas alkane hydroxylases are as distantly related to each other as to the remaining alkane hydroxylases. based on the observation that ... | 2002 | 11872725 |
| predominant growth of alcanivorax strains in oil-contaminated and nutrient-supplemented sea water. | we found that bacteria closely related to alcanivorax became a dominant bacterial population in petroleum-contaminated sea water when nitrogen and phosphorus nutrients were supplied in adequate quantity. the predominance of alcanivorax bacteria was demonstrated under three experimental conditions: (i) in batch cultures of sea water containing heavy oil; (ii) in columns packed with oil-coated gravel undergoing a continuous sea water flow; and (iii) in a large-scale tidal flux reactor that mimics ... | 2002 | 12000314 |
| oleiphilaceae fam. nov., to include oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. | a bacterial isolate, me102t, was obtained from an n-hexadecane enrichment culture of seawater/sediment samples collected in the harbour of messina (italy). this gram-negative, aerobic, motile, rod-shaped bacterium used a narrow spectrum of organic compounds, including aliphatic hydrocarbons, alkanoates and alkanoles, as carbon and energy sources. none of the sugars, organic acids or amino acids tested was used. during cultivation on n-alkanes as the sole source of carbon and energy, the cells fo ... | 2002 | 12054256 |
| robust hydrocarbon degradation and dynamics of bacterial communities during nutrient-enhanced oil spill bioremediation. | degradation of oil on beaches is, in general, limited by the supply of inorganic nutrients. in order to obtain a more systematic understanding of the effects of nutrient addition on oil spill bioremediation, beach sediment microcosms contaminated with oil were treated with different levels of inorganic nutrients. oil biodegradation was assessed respirometrically and on the basis of changes in oil composition. bacterial communities were compared by numerical analysis of denaturing gradient gel el ... | 2002 | 12406747 |
| [analysis of 16s rdna sequences and dna-dna hybridization of moderately halophilic bacteria from xinjiang region]. | based on the previous studies on numerical taxonomy and 16s rdna pcr-rflp analysis, the moderately halophilic bacteria isolated from xinjiang region constituted a new cluster, and the phylogenetic tree was constructed by comparing with the 16s rdna sequences of the other moderately halophilic bacteria species. in the phylogenetic tree, most of the reference strains were clustered in a group, and the similarity values of 16s rdna sequence were above 96%. however, ai-3, alcanivorax borkumensis and ... | 2002 | 12557387 |
| description of alcanivorax venustensis sp. nov. and reclassification of fundibacter jadensis dsm 1 21 78t (bruns and berthe-corti 1999) as alcanivorax jadensis comb. nov., members of the emended genus alcanivorax. | two strains of a novel bacterium were isolated independently of each other, from different depths in the mediterranean sea, within a time period of 7 months, using two different isolation approaches that were focused on different objectives. both strains, designated iso1 and iso4t, were halophilic, gram-negative, strictly aerobic, straight rods that were oxidase- and catalase-positive. both strains produced mucoid colonies in some defined minimal media and were able to grow with organic acids an ... | 2003 | 12656192 |
| oleispira antarctica gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from antarctic coastal sea water. | the taxonomic characteristics of two bacterial strains, rb-8(t) and rb-9, isolated from hydrocarbon-degrading enrichment cultures obtained from antarctic coastal marine environments (rod bay, ross sea), were determined. these bacteria were psychrophilic, aerobic and gram-negative with polar flagella. growth was not observed in the absence of nacl, occurred only at concentrations of na+ above 20 mm and was optimal at an nacl concentration of 3-5% (w/v). the major cellular fatty acids were monouns ... | 2003 | 12807200 |
| dynamics of microbial populations and strong selection for cycloclasticus pugetii following the nakhodka oil spill. | microbial population changes were monitored immediately after the nakhodka oil spill accident in january 1997 at the heavily oil-contaminated mikuni coast along the sea of japan. the total cell number was almost stable for one year at 2-5 x 10(5) cells ml(-1), while the relative occurrence of culturable heterotrophs and degraders of oil components such as c-heavy oil, kerosene, and n-tetradecane varied, showing a maximum (>50% of the total) immediately following the accident. gene amplification ... | 2003 | 12904913 |
| alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation. | alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. in order to understand why alcanivorax overcomes other bacteria under such cultural conditions, competition experiments between alcanivorax indigenous to seawater and the exogenous alkane-degrading marine bacterium, acinetobacter venetianus strain t4, were conducted. when oil-containing seawater supplemented with ni ... | 2003 | 12919410 |
| whole genome shotgun sequencing guided by bioinformatics pipelines--an optimized approach for an established technique. | while the sequencing of bacterial genomes has become a routine procedure at major sequencing centers, there are still a number of genome projects at small- or medium-size facilities. for these facilities a maximum of control over sequencing, assembling and finishing is essential. at the same time, facilities have to be able to co-operate at minimum costs for the overall project. we have established a pipeline for the distributed sequencing of alcanivorax borkumensis sk2, azoarcus sp. bh72, clavi ... | 2003 | 14651855 |
| genome sequence completed of alcanivorax borkumensis, a hydrocarbon-degrading bacterium that plays a global role in oil removal from marine systems. | in this paper, we provide background to the genome sequencing project of alcanivorax borkumensis, which is a marine bacterium that uses exclusively petroleum oil hydrocarbons as sources of carbon and energy (therefore designated "hydrocarbonoclastic"). it is found in low numbers in all oceans of the world and in high numbers in oil-contaminated waters. its ubiquity and unusual physiology suggest it is globally important in the removal of hydrocarbons from polluted marine systems. a functional ge ... | 2003 | 14651863 |
| thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. | an aerobic, heterotrophic, gram-negative, curved bacterial strain, designated mil-1t, was isolated by extinction dilution from an n-tetradecane enrichment culture that was established from sea water/sediment samples collected in the harbour of milazzo, italy. in the primary enrichment, the isolate formed creamy-white, medium-sized colonies on the surface of the agar. the isolate did not grow in the absence of nacl; growth was optimal at 2.7% nacl. only a narrow spectrum of organic compounds, inc ... | 2004 | 14742471 |
| cloning and functional analysis of alkb genes in alcanivorax borkumensis sk2. | alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. to identify the genes responsible for alkane degradation in this organism, two putative genes for alkane hydroxylases were cloned from alcanivorax borkumensis sk2. they were named alkb1 and alkb2. these genes were subsequently disrupted in a. borkumensis sk2, and the growth phenotypes of the disruptants were examine ... | 2004 | 14871203 |
| characterization of two alkane hydroxylase genes from the marine hydrocarbonoclastic bacterium alcanivorax borkumensis. | the marine gamma-proteobacterium alcanivorax borkumensis is highly specialized in the assimilation of aliphatic hydrocarbons, and makes up a large part of the biomass in oil-polluted marine environments. in addition to the previously identified alkane hydroxylase alkb1, a second alkane hydroxylase (alkb2) showing 65% identity to the pseudomonas aeruginosa alkb2 alkane hydroxylase was identified. unlike alkb1, alkb2 is not flanked by genes involved in alkane metabolism. heterologous expression of ... | 2004 | 14871210 |
| bacterial community dynamics and hydrocarbon degradation during a field-scale evaluation of bioremediation on a mudflat beach contaminated with buried oil. | a field-scale experiment with a complete randomized block design was performed to study the degradation of buried oil on a shoreline over a period of almost 1 year. the following four treatments were examined in three replicate blocks: two levels of fertilizer treatment of oil-treated plots, one receiving a weekly application of liquid fertilizer and the other treated with a slow-release fertilizer; and two controls, one not treated with oil and the other treated with oil but not with fertilizer ... | 2004 | 15128509 |
| microbial communities in oil-contaminated seawater. | although diverse bacteria capable of degrading petroleum hydrocarbons have been isolated and characterized, the vast majority of hydrocarbon-degrading bacteria, including anaerobes, could remain undiscovered, as a large fraction of bacteria inhabiting marine environments are uncultivable. using culture-independent rrna approaches, changes in the structure of microbial communities have been analyzed in marine environments contaminated by a real oil spill and in micro- or mesocosms that mimic such ... | 2004 | 15193328 |
| identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases. | selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (ahs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. first, substrate range mutants of the pseudomonas putida gpo1 and alcanivorax borkumensis ap1 medium-chain-length ahs were obtained by selection experiments with a specially constructed host. in all mutants able to oxidize alkanes longer than c13, w55 (in the case of p. put ... | 2005 | 15601691 |
| mercury adaptation among bacteria from a deep-sea hydrothermal vent. | since deep-sea hydrothermal vent fluids are enriched with toxic metals, it was hypothesized that (i) the biota in the vicinity of a vent is adapted to life in the presence of toxic metals and (ii) metal toxicity is modulated by the steep physical-chemical gradients that occur when anoxic, hot fluids are mixed with cold oxygenated seawater. we collected bacterial biomass at different distances from a diffuse flow vent at 9 degrees n on the east pacific rise and tested these hypotheses by examinin ... | 2005 | 15640191 |
| [preliminary research on bacterial diversity of parece vela basin, pacific ocean by culture-independent method]. | the environmental dna was directly extracted from the sediment in parece vela basin, pacific ocean, at a depth of 5010 m. bacterial 16s rrna gene library of 32 clones was generated using bacterial universal primers and 16s rdna sequences were analyzed phylogenetically. 17 phylotypes were obtained. the library was dominated by gamma-proteobacteria, alpha-proteobacteria and marine uncultured bacteria. sixty-two percent of the cloned sequences was highly related to the known bacteria in the genus h ... | 2005 | 15847151 |
| alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. | two bacterial strains, b-5(t) and no1a, were isolated from the surface water of the bohai sea and deep-sea sediment of the east pacific ocean, respectively. both strains were halophilic, aerobic, gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. they grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. on the basis of 16s rrna gene sequence similarity, strains b-5(t) and no1a were shown to belong to the gamma-proteobacteria. hi ... | 2005 | 15879252 |
| natural microbial diversity in superficial sediments of milazzo harbor (sicily) and community successions during microcosm enrichment with various hydrocarbons. | hydrocarbon-contaminated superficial sediments collected from the harbor of milazzo (tirrenean sea, northern sicily), a zone strongly affected by anthropogenic activities, were examined for in situ biodegradative capacities. a culture-independent molecular phylogenetic approach was used to study the influence of hydrocarbon and nutrient addition on the activity and diversity of the indigenous microbiota during a microcosm evaluation. the autochthonous microbial community in non-polluted sediment ... | 2005 | 16104865 |
| functional expression system for cytochrome p450 genes using the reductase domain of self-sufficient p450rhf from rhodococcus sp. ncimb 9784. | cytochrome p450rhf from rhodococcus sp. ncimb 9784 is a self-sufficient p450 monooxygenase. we report here a simple system for the functional expression of various p450 genes using the reductase domain of this p450rhf, which comprises flavin mononucleotide- and nicotinamide adenine dinucleotide phosphate binding motifs and a [2fe2s] ferredoxin-like center. vector pred was constructed, which carried the t7 promoter, cloning sites for a p450, a linker sequence, and the p450rhf reductase domain, in ... | 2006 | 16195793 |
| isolation and functional analysis of cytochrome p450 cyp153a genes from various environments. | the cytochrome p450 cyp153 family is thought to mediate the terminal hydroxylation reactions of n-alkanes. we isolated 16 new p450 cyp153a genes (central region) from various environments such as petroleum-contaminated soil and groundwater, as well as one from the n-alkane-degrading bacterium alcanivorax borkumensis sk2 (designated p450balk). the sequences of the new p450 genes were extended by pcr to generate full-length chimeric p450 genes, using the n- and c-terminal domains of p450balk. a di ... | 2005 | 16377903 |
| a new method for the detection of alkane-monooxygenase homologous genes (alkb) in soils based on pcr-hybridization. | an improved method was developed that allowed the specific detection of the gene alkb (coding for the rubredoxin dependent alkane monooxygenase) from bacteria without any obvious strain specific discrimination using a combination of pcr and hybridization. this approach enabled a fast culture-independent monitoring of environmental samples for the occurrence of alkb, and an estimation of the gene copy number and the genetic diversity. both parameters provide useful informations for an assessment ... | 2006 | 16522338 |
| population dynamics within a microbial consortium during growth on diesel fuel in saline environments. | the diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near comodoro rivadavia in patagonia (argentina) were investigated. community shifts during long-term incubation with diesel fuel at four salinities between 0 and 20% nacl were monitored by single-strand conformation polymorphism community fingerprinting of the pcr-amplified v4-v5 region of the 16s rrna genes. information obtained by this qualitative approach was extended by ... | 2006 | 16672500 |
| proteomic insights into metabolic adaptations in alcanivorax borkumensis induced by alkane utilization. | alcanivorax borkumensis is a ubiquitous marine petroleum oil-degrading bacterium with an unusual physiology specialized for alkane metabolism. this "hydrocarbonoclastic" bacterium degrades an exceptionally broad range of alkane hydrocarbons but few other substrates. the proteomic analysis presented here reveals metabolic features of the hydrocarbonoclastic lifestyle. specifically, hexadecane-grown and pyruvate-grown cells differed in the expression of 97 cytoplasmic and membrane-associated prote ... | 2006 | 16707669 |
| characterization of hydrocarbonoclastic bacterial communities from mangrove sediments in guanabara bay, brazil. | hydrocarbonoclastic bacterial communities inhabiting mangrove sediments were characterized by combining molecular and culture-dependent approaches. surface sediments were collected at two sampling sites in guanabara bay (rio de janeiro, brazil) and used to inoculate in vitro enrichment cultures containing crude oil to obtain hydrocarbonoclastic bacterial consortia. in parallel, in situ mesocosms (located in the guapimirim mangrove) were contaminated with petroleum. comparison of bacterial commun ... | 2006 | 16815684 |
| predominant growth of alcanivorax during experiments on "oil spill bioremediation" in mesocosms. | mesocosm experiments were performed to study the changes on bacterial community composition following oil spill in marine environment. the analysis of 16s crdna revealed a shift in the structure of initial bacterial population that was drastically different from that one measured after 15 days. the results showed that, after 15 days, bacteria closely related to the genus alcanivorax became the dominant group of bacterial community in petroleum-contaminated sea water nitrogen and phosphorus amend ... | 2007 | 16831537 |
| genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium alcanivorax borkumensis. | alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. although barely detectable in unpolluted environments, a. borkumensis becomes the dominant microbe in oil-polluted waters. a. borkumensis sk2 has a streamlined genome with a paucity of mobile genetic elements and energy generation-related genes, but with a plethora of genes accounting for its wide hydrocarbon substrate range and efficient oil-degradation capabilitie ... | 2006 | 16878126 |
| mutation in a "tesb-like" hydroxyacyl-coenzyme a-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by alcanivorax borkumensis sk2. | a novel mutant of the marine oil-degrading bacterium alcanivorax borkumensis sk2, containing a mini-tn5 transposon disrupting a "tesb-like" acyl-coenzyme a (coa) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (pha), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. the tesb-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-coas and thus represents a hydroxyacyl-coa-specif ... | 2006 | 16997960 |
| phylogenetic diversity and activity of aerobic heterotrophic bacteria from a hypersaline oil-polluted microbial mat. | the diversity and function of aerobic heterotrophic bacteria (ahb) in cyanobacterial mats have been largely overlooked. we used culture-dependent and molecular techniques to explore the species diversity, degradative capacities and functional guilds of ahb in the photic layer (2mm) of an oil-polluted microbial mat from saudi arabia. enrichment isolation was carried out at different salinities (5% and 12%) and temperatures (28 and 45 degrees c) and on various substrates (acetate, glycolate, spiru ... | 2007 | 17056222 |
| analysis of storage lipid accumulation in alcanivorax borkumensis: evidence for alternative triacylglycerol biosynthesis routes in bacteria. | marine hydrocarbonoclastic bacteria, like alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor challenging those bacteria in their natural marine habitats. here we report on the analysis of storage lipid bi ... | 2007 | 17122340 |
| determining the identity and roles of oil-metabolizing marine bacteria from the thames estuary, uk. | crude oil is a complex mixture of different hydrocarbons. while diverse bacterial communities can degrade oil, the specific roles of individual members within such communities remain unclear. to identify the key bacterial taxa involved in aerobic degradation of specific hydrocarbons, microcosm experiments were established using seawater from stanford le hope, thames estuary, uk, adjacent to a major oil refinery. in all microcosms, hydrocarbon degradation was significant within 10 weeks, ranging ... | 2007 | 17227421 |
| isolation of methylophaga spp. from marine dimethylsulfide-degrading enrichment cultures and identification of polypeptides induced during growth on dimethylsulfide. | dimethylsulfide (dms)-degrading enrichment cultures were established from samples of coastal seawater, nonaxenic emiliania huxleyi cultures, and mixed marine methyl halide-degrading enrichment cultures. bacterial populations from a broad phylogenetic range were identified in the mixed dms-degrading enrichment cultures by denaturing gradient gel electrophoresis (dgge). sequences of dominant dgge bands were similar to those of members of the genera methylophaga and alcanivorax. several closely rel ... | 2007 | 17322322 |
| obligate oil-degrading marine bacteria. | over the past few years, a new and ecophysiologically unusual group of marine hydrocarbon-degrading bacteria - the obligate hydrocarbonoclastic bacteria (ohcb) - has been recognized and shown to play a significant role in the biological removal of petroleum hydrocarbons from polluted marine waters. the introduction of oil or oil constituents into seawater leads to successive blooms of a relatively limited number of indigenous marine bacterial genera--alcanivorax, marinobacter, thallassolituus, c ... | 2007 | 17493798 |
| efficacy of intervention strategies for bioremediation of crude oil in marine systems and effects on indigenous hydrocarbonoclastic bacteria. | there is little information on how different strategies for the bioremediation of marine oil spills influence the key indigenous hydrocarbon-degrading bacteria (hydrocarbonoclastic bacteria, hcb), and hence their remediation efficacy. therefore, we have used quantitative polymerase chain reaction to analyse changes in concentrations of hcb in response to intervention strategies applied to experimental microcosms. biostimulation with nutrients (n and p) produced no measurable increase in either b ... | 2007 | 17504493 |
| alcanivorax balearicus sp. nov., isolated from lake martel. | a bacterial strain designated macl04(t) was isolated from lake martel, a subterraneous saline lake in mallorca (spain). the complete 16s rrna gene sequence of this strain showed nearly 100 % similarity to that of alcanivorax dieselolei b-5(t). despite this high similarity, strain macl04(t) showed phenotypic, chemotaxonomic and molecular differences with respect to a. dieselolei, indicating that strain macl04(t) represents a separate species. cells of strain macl04(t) were motile by means of a si ... | 2007 | 17551053 |
| litoricolaceae fam. nov., to include litoricola lipolytica gen. nov., sp. nov., a marine bacterium belonging to the order oceanospirillales. | a gram-negative, non-motile, chemoheterotrophic, facultatively aerobic, short-rod-shaped bacterium, designated imcc1097(t), was isolated from coastal seawater (10 m depth) of the east sea, korea. the temperature, ph and nacl ranges for growth were 15-30 degrees c, ph 5.0-10.0 and 1.5-10 % nacl. the colonies of the strain were very small, having a mean diameter of 0.05 mm. 16s rrna gene sequence data indicated that the strain was most closely related to genera within the class gammaproteobacteria ... | 2007 | 17684259 |
| global features of the alcanivorax borkumensis sk2 genome. | the global feature of the completely sequenced alcanivorax borkumensis sk2 type strain chromosome is its symmetry and homogeneity. the origin and terminus of replication are located opposite to each other in the chromosome and are discerned with high signal to noise ratios by maximal oligonucleotide usage biases on the leading and lagging strand. genomic dna structure is rather uniform throughout the chromosome with respect to intrinsic curvature, position preference or base stacking energy. the ... | 2008 | 18081853 |
| structural determination and proposed biosynthesis of alcanivorone, a novel alpha-pyrone produced by alcanivorax jadensis. | a novel alpha-pyrone designated as alcanivorone was found in a culture broth of the marine bacterium, alcanivorax jadensis, and its structure was determined by an analysis of 1d nmr, 2d nmr and ms data. alcanivorone was produced by a. jadensis only when sodium pyruvate was added to the culture medium as a carbon source. incorporation experiments using stable isotope-labeled pyruvate indicated that alcanivorone was biosynthesized from four molecules of pyruvate. | 2008 | 18408325 |
| a pyrene-degrading consortium from deep-sea sediment of the west pacific and its key member cycloclasticus sp. p1. | a pyrene-degrading bacterial consortium was obtained from deep-sea sediments of the pacific ocean. the consortium degraded many kinds of polycyclic aromatic hydrocarbons (pahs), including naphthalene, phenanthrene, pyrene, acenaphthene, fluorene, anthracene, fluoranthene, 2-methylnaphthalene and 2,6-dimethylnaphthalene, but it did not grow with chrysene and benzo[alpha]pyrene. with methods of plate cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), 72 b ... | 2008 | 18430013 |
| biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the middle atlantic ridge. | the bacteria involved in the biodegradation of polycyclic aromatic hydrocarbons (pahs) in deep sea subsurface environments are largely unknown. in order to reveal their biodiversity, sediments from 2.2 m under the bottom surface at a water depth of 3542 m were sampled on the middle atlantic ridge with a gravity column sampler. the sediments were promptly enriched with either crude oil or a mixture of pahs (naphthalene, phenanthrene and pyrene) as the sole carbon source, and further enriched with ... | 2008 | 18445026 |
| phylogenetic analyses of ribosomal dna-containing bacterioplankton genome fragments from a 4000 m vertical profile in the north pacific subtropical gyre. | high-throughput identification of rrna gene-containing clones in large insert metagenomic libraries is difficult, because of the high background of host ribosomal rna (rrna) and rrna genes. to address this challenge, a membrane hybridization method was developed to identify all bacterial small subunit rrna-containing fosmid clones of microbial community dna from seven different depths in the north pacific subtropical gyre. out of 101,376 clones screened, 751 rdna-containing clones were identifie ... | 2008 | 18494796 |
| niche-specificity factors of a marine oil-degrading bacterium alcanivorax borkumensis sk2. | alcanivorax borkumensis strain sk2 is a cosmopolitan hydrocarbonoclastic marine bacterium, with a specialized metabolism adapted to the degradation of petroleum oil hydrocarbons. transposon mutagenesis was used for functional genome analysis of alcanivorax sk2 to reveal the genetic basis of other environmentally relevant phenotypes, such as biofilm formation, adaptation to uv exposure, and to growth at either low temperature or high salinity. forty-eight relevant transposon mutants deficient in ... | 2008 | 18557784 |
| distribution of oil-degrading bacteria in coastal seawater, toyama bay, japan. | oil-degrading bacteria are considered to play an important role in the biodegradation of spilled or released oil in the sea. the distribution of indigenous oil-degrading bacteria in the coastal seawater of toyama bay, japan, was examined. surface seawater samples with or without oil film in fishing port were analyzed by denaturing gradient gel electrophoresis (dgge) of the pcr-amplified v3 region of bacterial 16s rdna. sequence analysis revealed that several dgge bands clearly detected only in s ... | 2008 | 18561305 |
| construction of a consortium comprising ammonia-oxidizing bacteria and denitrifying bacteria isolated from marine sediment. | a unique consortium of ammonia-oxidizing bacteria (aob) and denitrifying bacteria was obtained via a long-term, 3-step cultivation of isolates from organically-enriched marine sediment. we developed this microbial consortium for possible applications in the remediation of degraded habitats in closed aquaculture or other aquatic environments via microbial degradation. analysis of media components found definitive evidence of nitrogen removal via the coupling of ammonia-oxidation and denitrificati ... | 2008 | 18839627 |
| tree of life based on genome context networks. | efforts in phylogenomics have greatly improved our understanding of the backbone tree of life. however, due to the systematic error in sequence data, a sequence-based phylogenomic approach leads to well-resolved but statistically significant incongruence. thus, independent test of current phylogenetic knowledge is required. here, we have devised a distance-based strategy to reconstruct a highly resolved backbone tree of life, on the basis of the genome context networks of 195 fully sequenced rep ... | 2008 | 18852873 |
| isolation and characterization of biosurfactant-producing alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis. | biosurfactant-producing bacteria belonging to the genera alcanivorax, cobetia and halomonas were isolated from marine sediments with a history of hydrocarbon exposure (aristizábal and gravina peninsulas, argentina). two alcanivorax isolates were found to form naturally occurring consortia with strains closely related to pseudomonas putida and microbacterium esteraromaticum. alkane hydroxylase gene analysis in these two alcanivorax strains resulted in the identification of two novel alkb genes, s ... | 2009 | 18983915 |
| new alk genes detected in antarctic marine sediments. | alkane monooxygenases (alk) are the key enzymes for alkane degradation. in order to understand the dispersion and diversity of alk genes in antarctic marine environments, this study analysed by clone libraries the presence and diversity of alk genes (alkb and alkm) in sediments from admiralty bay, king george island, peninsula antarctica. the results show a differential distribution of alk genes between the sites, and the predominant presence of new alk genes, mainly in the pristine site. sequen ... | 2009 | 19207566 |
| [diversity of bacteria isolated from the south china sea sediments]. | to isolate bacteria from nansha area of south china sea, | 2008 | 19271531 |
| populations of heavy fuel oil-degrading marine microbial community in presence of oil sorbent materials. | to investigate the feasibility of applying sorbent material x-oil in marine oil spill mitigation and to survey the interactions of oil, bacteria and sorbent. | 2009 | 19302488 |
| microbial consortia in mesocosm bioremediation trial using oil sorbents, slow-release fertilizer and bioaugmentation. | an experimental prototype oil boom including oil sorbents, slow-release fertilizers and biomass of the marine oil-degrading bacterium, alcanivorax borkumensis, was applied for sorption and degradation of heavy fuel oil in a 500-l mesocosm experiment. fingerprinting of dna and small subunit rrna samples for microbial activity conducted to study the changes in microbial communities of both the water body and on the oil sorbent surface showed the prevalence of a. borkumensis on the surface of the o ... | 2009 | 19496821 |
| alcanivorax hongdengensis sp. nov., an alkane-degrading bacterium isolated from surface seawater of the straits of malacca and singapore, producing a lipopeptide as its biosurfactant. | a taxonomic study was carried out on strain a-11-3(t), which was isolated from an oil-enriched consortia from the surface seawater of hong-deng dock in the straits of malacca and singapore. cells were aerobic, gram-negative, non-spore-forming irregular rods. the strain was catalase- and oxidase-negative. it grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. 16s rrna gene sequence comparisons showed that strain a-11-3(t) was most closely related to the t ... | 2009 | 19502338 |
| oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment. | petroleum-hydrocarbon-degrading bacteria were obtained after enrichment on crude oil (as a 'chocolate mousse') in a continuous supply of indonesian seawater amended with nitrogen, phosphorus and iron nutrients. they were related to alcanivorax and marinobacter strains, which are ubiquitous petroleum-hydrocarbon-degrading bacteria in marine environments, and to oceanobacter kriegii (96.4-96.5 % similarities in almost full-length 16s rrna gene sequences). the oceanobacter-related bacteria showed h ... | 2009 | 19541999 |
| isolation and characterization of a novel biosurfactant produced by hydrocarbon-degrading bacterium alcanivorax dieselolei b-5. | our goal was to identify a novel biosurfactant produced by a marine oil-degrading bacterium. | 2010 | 19732216 |
| isolation of an alkane-degrading alcanivorax sp. strain 2b5 and cloning of the alkb gene. | a gram-negative coccus, strain 2b5, was isolated from the sea mud of the crude oil-polluted donghai area in china and identified as alcanivorax sp. based on its physiological characteristics and analysis of its 16s rrna gene sequence. strain 2b5 was able to degrade c(13)-c(30)n-alkanes and branched alkanes (pristane and phytane) from crude oil as the sole carbon source. the optimal temperature and ph for strain 2b5 growth and octadecane degradation were 30-37 degrees c and ph 6.0-7.0, respective ... | 2010 | 19733061 |
| arenicella xantha gen. nov., sp. nov., a gammaproteobacterium isolated from a marine sandy sediment. | a gram-negative, aerobic, yellow-pigmented, rod-shaped, non-motile bacterium, strain kmm 3895t, was isolated from a marine sandy sample collected offshore from the sea of japan. phylogenetic analysis based on the 16s rrna gene sequence placed strain kmm 3895t in the class gammaproteobacteria, forming a separate branch, sharing 89.5 % sequence similarity with nitrincola lacisaponensis 4cat and 88-87% similarity with the other members of the cluster, including members of kangiella, spongiibacter, ... | 2010 | 19767361 |
| denitrifying activity and homologous enzyme analysis of alcanivorax dieselolei strain n1203. | an alcanivorax dieselolei strain, termed strain n1203, was isolated from the consortia of ammonia-oxidizing bacteria (aob) combined with denitrifying bacteria from our previous study and was shown to have ability to reduce nitrate to nitrite to either nitrous oxide or molecular nitrogen. analysis of 16s rrna gene sequences established strain n1203 as a member of the species alcanivorax dieselolei. in addition, the ability of strain n1203 to utilize various organic substrates as the sole carbon s ... | 2009 | 19785282 |
| changes of microbial community structures and functional genes during biodegradation of phenolic compounds under high salt condition. | the changes of microbial community structures and functional genes during the biodegradation of single phenol and phenol plus p-cresol under high salt condition were explored. it was found that the phenol-fed system (pfs) exhibited stronger degrading abilities and more stable biomass than that of the phenol plus p-cresol-fed system (pcfs). the microbial community structures were revealed by a modern dna fingerprint technique, ribosomal intergenic spacer analysis (risa). the results indicated tha ... | 2009 | 19803089 |
| [isolation, identification and diversity analysis of petroleum-degrading bacteria in shengli oil field wetland soil]. | the petroleum-degrading bacteria in shengli oil field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by pcr-dgge (denaturing gradient gel electrophoresis). a total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of c12-c26 petroleum hydrocarbon, with a degradation rate of > 90%. these petroleum degraders were phylogeneticly identified a ... | 2009 | 19803182 |
| characterization of marine isoprene-degrading communities. | isoprene is a volatile and climate-altering hydrocarbon with an atmospheric concentration similar to that of methane. it is well established that marine algae produce isoprene; however, until now there was no specific information about marine isoprene sinks. here we demonstrate isoprene consumption in samples from temperate and tropical marine and coastal environments, and furthermore show that the most rapid degradation of isoprene coincides with the highest rates of isoprene production in estu ... | 2009 | 19807779 |
| [predominant strains of polycyclic aromatic hydrocarbon-degrading consortia from deep sea of the middle atlantic ridge]. | in order to identify the predominant strains of polycyclic aromatic hydrocarbon (pah)-degrading consortia harboring in sea water and surface sediment collected from deep sea of the middle atlantic ridge. | 2009 | 19873754 |
| analysis of lipid export in hydrocarbonoclastic bacteria of the genus alcanivorax: identification of lipid export-negative mutants of alcanivorax borkumensis sk2 and alcanivorax jadensis t9. | triacylglycerols (tags), wax esters (wes), and polyhydroxyalkanoates (phas) are the major hydrophobic compounds synthesized in bacteria and deposited as cytoplasmic inclusion bodies when cells are cultivated under imbalanced growth conditions. the intracellular occurrence of these compounds causes high costs for downstream processing. alcanivorax species are able to produce extracellular lipids when the cells are cultivated on hexadecane or pyruvate as the sole carbon source. in this study, we d ... | 2010 | 19933359 |
| isolation of alkane-degrading bacteria from deep-sea mediterranean sediments. | to isolate and identify alkane-degrading bacteria from deep-sea superficial sediments sampled at a north-western mediterranean station. | 2010 | 19943883 |
| production of a recombinant alkane hydroxylase (alkb2) from alcanivorax borkumensis. | alcanivorax borkumensis is an oil-degrading marine bacterium. its genome contains genes coding for three cytochrome p450s and two integral membrane alkane hydroxylases (alkb1 & alkb2), all assumed to perform hydroxylation of different linear or branched alkanes. although, the sequence of alkb2 has been determined, the molecular characterization and the substrate specificity of alkb2 require more precise investigation. in this study, alkb2 from a. borkumensis sk2 was expressed in escherichia coli ... | 2010 | 19953301 |
| gene diversity of cyp153a and alkb alkane hydroxylases in oil-degrading bacteria isolated from the atlantic ocean. | alkane hydroxylases, including the integral-membrane non-haem iron monooxygenase (alkb) and cytochrome p450 cyp153 family, are key enzymes in bacterial alkane oxidation. although both genes have been detected in a number of bacteria and environments, knowledge about the diversity of these genes in marine alkane-degrading bacteria is still limited, especially in pelagic areas. in this report, 177 bacterial isolates, comprising 43 genera, were obtained from 18 oil-degrading consortia enriched from ... | 2010 | 20148932 |
| isolation and characterization of a mutant of the marine bacterium alcanivorax borkumensis sk2 defective in lipid biosynthesis. | in many microorganisms, the key enzyme responsible for catalyzing the last step in triacylglycerol (tag) and wax ester (we) biosynthesis is an unspecific acyltransferase which is also referred to as wax ester synthase/acyl coenzyme a (acyl-coa):diacylglycerol acyltransferase (ws/dgat; atfa). the importance and function of two atfa homologues (atfa1 and atfa2) in the biosynthesis of tags and wes in the hydrocarbon-degrading marine bacterium alcanivorax borkumensis sk2 have been described recently ... | 2010 | 20305021 |
| occurrence, production, and export of lipophilic compounds by hydrocarbonoclastic marine bacteria and their potential use to produce bulk chemicals from hydrocarbons. | petroleum (or crude oil) is a complex mixture of hydrocarbons. annually, millions of tons of crude petroleum oil enter the marine environment from either natural or anthropogenic sources. hydrocarbon-degrading bacteria (hdb) are able to assimilate and metabolize hydrocarbons present in petroleum. crude oil pollution constitutes a temporary condition of carbon excess coupled to a limited availability of nitrogen that prompts marine oil-degrading bacteria to accumulate storage compounds. storage l ... | 2010 | 20354694 |
| [synergic effect of marine obligate hydrocarbonoclastic bacteria in oil biodegradation]. | in order to study the synergic effect of two marine obligate hydrocarbonoclastic bacteria in the oil biodegradation process. | 2010 | 20499640 |
| microbial community structure of a heavy fuel oil-degrading marine consortium: linking microbial dynamics with polycyclic aromatic hydrocarbon utilization. | a marine microbial consortium obtained from a beach contaminated by the prestige oil spill proved highly efficient in removing the different hydrocarbon families present in this heavy fuel oil. seawater cultures showed a complete removal of all the linear and branched alkanes, an extensive attack on three to five-ring polycyclic aromatic hydrocarbons [pahs; including anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, and benzo(a)pyrene] (30-100%), and a considerable depletion of the ... | 2010 | 20528986 |
| identities of epilithic hydrocarbon-utilizing diazotrophic bacteria from the arabian gulf coasts, and their potential for oil bioremediation without nitrogen supplementation. | gravel particles from four sites along the arabian gulf coast in autumn, winter, and spring were naturally colonized with microbial consortia containing between 7 and 400 × 10(2) cm(-2) of cultivable oil-utilizing bacteria. the 16s rrna gene sequences of 70 representatives of oil-utilizing bacteria revealed that they were predominantly affiliated with the gammaproteobacteria and the actinobacteria. the gammaproteobacteria comprised among others, the genera pseudomonas, pseudoalteromonas, shewane ... | 2010 | 20567813 |
| alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. | a taxonomic study was carried out on a novel bacterial strain, designated w11-5(t), which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the pacific ocean. the isolate was gram-reaction-negative and oxidase- and catalase-positive. growth was observed in 0.5-12 % (w/v) nacl and at 10-42 °c. on the basis of 16s rrna gene sequence analysis, strain w11-5(t) was shown to belong to the genus alcanivorax with a close relation to a. dieselolei b-5(t) (93.9 % 16s rrna ... | 2010 | 20584816 |
| diversity and abundance of oil-degrading bacteria and alkane hydroxylase (alkb) genes in the subtropical seawater of xiamen island. | in this report, the diversity of oil-degrading bacteria and alkb gene was surveyed in the seawater around xiamen island. forty-four isolates unique in 16s rrna sequence were obtained after enrichment with crude oil. most of the obtained isolates exhibited growth with diesel oil and crude oil. alkb genes were positively detected in 16 isolates by degenerate polymerase chain reaction (pcr). and for the first time, alkb genes were found in bacteria of gallaecimonas, castellaniella, paracoccus, and ... | 2010 | 20683589 |
| [identification of alma genes involved in long-chain alkane degradation by alcanivorax hongdengensis a-11-3]. | the aim of this study is to identify of genes involved in long-chain (lc) alkane degradation in alcanivorax hongdengensis a-11-3. | 2010 | 20931873 |
| [diversity of oil-degrading bacteria isolated form the indian ocean sea surface]. | in order to investigate the diversity of oil-degrading bacteria in the surface seawater across the india ocean, and to obtain new oil-degrading bacteria. | 2010 | 21090262 |
| re-use of remediated soils for the bioremediation of waste oil sludge. | we investigated the possibility of re-using remediated soils for new bioremediation projects by spiking these soils with waste oil sludge in laboratory based microcosms. the level of total petroleum hydrocarbon (tph) reduction was high (>80%) in naturally attenuated microcosms and was not significantly improved by biostimulation, bioaugmentation and the combined treatment of bioaugmentation and biostimulation by week 12. this indicated that the observed tph reduction might have been related to t ... | 2010 | 21115217 |
| a halotolerant alcanivorax sp. strain with potential application in saline soil remediation. | biodegradation of petroleum compounds in saline environments seems intricate and needs more attention. in this study, tetracosane was used to enrich alkane-degrading bacteria from oil-contaminated saline soils. among the isolates, strain qtet3, with the highest 16s rrna gene sequence similarity to alcanivorax dieselolei b-5(t), was able to grow at a wide range of nacl concentrations and was shown by gc analysis to degrade more than 90% of tetracosane in 10 days. this strain has at least two alkb ... | 2010 | 21153810 |
| multiple alkane hydroxylase systems in a marine alkane degrader, alcanivorax dieselolei b-5. | alcanivorax dieselolei strain b-5 is a marine bacterium that can utilize a broad range of n-alkanes (c(5) -c(36) ) as sole carbon source. however, the mechanisms responsible for this trait remain to be established. here we report on the characterization of four alkane hydroxylases from a. dieselolei, including two homologues of alkb (alkb1 and alkb2), a cyp153 homologue (p450), as well as an alma-like (alma) alkane hydroxylase. heterologous expression of alkb1, alkb2, p450 and alma in pseudomona ... | 2011 | 21261799 |
| transcriptional profiling of the marine oil-degrading bacterium alcanivorax borkumensis during growth on n-alkanes. | the marine oil-degrading bacterium alcanivorax borkumensis sk2 has attracted significant interest due to its hydrocarbonoclastic lifestyle, its alkane-centered metabolism, and for playing an important ecological role in cleaning up marine oil spills. in this study, we used microarray technology to characterize the transcriptional responses of a. borkumensis to n-hexadecane exposure as opposed to pyruvate, which led to the identification of a total of 220 differentially expressed genes, with 109 ... | 2011 | 21470299 |
| polyphasic approach for assessing changes in an autochthonous marine bacterial community in the presence of prestige fuel oil and its biodegradation potential. | a laboratory experiment was conducted to identify key hydrocarbon degraders from a marine oil spill sample (prestige fuel oil), to ascertain their role in the degradation of different hydrocarbons, and to assess their biodegradation potential for this complex heavy oil. after a 17-month enrichment in weathered fuel, the bacterial community, initially consisting mainly of methylophaga species, underwent a major selective pressure in favor of obligate hydrocarbonoclastic microorganisms, such as al ... | 2011 | 21562979 |
| are alkane hydroxylase genes (alkb) relevant to assess petroleum bioremediation processes in chronically polluted coastal sediments? | the diversity of alkb-related alkane hydroxylase sequences and the relationship between alkb gene expression and the hydrocarbon contamination level have been investigated in the chronically polluted etang-de-berre sediments. for this purpose, these sediments were maintained in microcosms and submitted to a controlled oil input miming an oil spill. new degenerated pcr primers targeting alkb-related alkane hydroxylase sequences were designed to explore the diversity and the expression of these ge ... | 2011 | 21660544 |
| voltage-gated sodium channel (nav) protein dissection creates a set of functional pore-only proteins. | many voltage-gated ion channel (vgic) superfamily members contain six-transmembrane segments in which the first four form a voltage-sensing domain (vsd) and the last two form the pore domain (pd). studies of potassium channels from the vgic superfamily together with identification of voltage-sensor only proteins have suggested that the vsd and the pd can fold independently. whether such transmembrane modularity is common to other vgic superfamily members has remained untested. here we show, usin ... | 2011 | 21746903 |
| rubredoxin reductase from alcanivorax borkumensis: expression and characterization. | oil pollution is an environmental problem of increasing importance. alcanivorax borkumensis, with a high potential for biotechnological applications, is a key marine hydrocarbonoclastic bacterium and plays a critical role in the bioremediation of oil-polluted marine systems. in oil degrading bacteria, the first step of alkane degradation is catalyzed by a monooxygenase. the reducing electrons are tunneled from nad(p)h via rubredoxin, one of the most primitive metalloproteins, to the hydroxylase. ... | 2011 | 21714118 |
| development of bioreporter assays for the detection of bioavailability of long-chain alkanes based on the marine bacterium alcanivorax borkumensis strain sk2. | long-chain alkanes are a major component of crude oil and therefore potentially good indicators of hydrocarbon spills. here we present a set of new bacterial bioreporters and assays that allow to detect long-chain alkanes. these reporters are based on the regulatory protein alks and the alkb1 promoter from alcanivorax borkumensis sk2, a widespread alkane degrader in marine habitats. escherichia coli cells with the reporter construct reacted strongly to octane in short-term (6 h) aqueous suspensi ... | 2011 | 21895911 |
| Genes involved in alkane degradation in the Alcanivorax hongdengensis strain A-11-3. | Alcanivorax hongdengensis A-11-3 is a newly identified type strain isolated from the surface water of the Malacca and Singapore Straits that can degrade a wide range of alkanes. To understand the degradation mechanism of this strain, the genes encoding alkane hydroxylases were obtained by PCR screening and shotgun sequencing of a genomic fosmid library. Six genes involved in alkane degradation were found, including alkB1, alkB2, p450-1, p450-2, p450-3 and almA. Heterogeneous expression analysis ... | 2011 | 22207216 |
| Cloning and expression of alkane hydroxylase-1 from Alcanivorax borkumensis in Escherichia coli. | Enzymes with hydroxylating activity on alkanes have potential application as biotransformation catalysts in chemical and pharmaceutical industry. Genome of Alcanivorax borkumensis, a marine bacterium with hydrocarbon dissimilation activity, contains at least two P450 monooxygenases and two nonheme monooxygenases, AlkB1 and AlkB2, respectively. Presumably, all these enzymes possess alkane hydroxylating activity. Both AlkB1 and AlkB2 are membrane proteins. Two accessory proteins, rubredoxin and ru ... | 2011 | 22064680 |
| Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. | Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ... | 2012 | 22130193 |
| hydrocarbon-degrading bacteria and the bacterial community response in gulf of mexico beach sands impacted by the deepwater horizon oil spill. | a significant portion of oil from the recent deepwater horizon (dh) oil spill in the gulf of mexico was transported to the shoreline, where it may have severe ecological and economic consequences. the objectives of this study were (i) to identify and characterize predominant oil-degrading taxa that may be used as model hydrocarbon degraders or as microbial indicators of contamination and (ii) to characterize the in situ response of indigenous bacterial communities to oil contamination in beach e ... | 2011 | 21948834 |