| application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops. | miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ... | 2010 | 20638415 |
| analysis of the solvent accessibility of cysteine residues on maize rayado fino virus virus-like particles produced in nicotiana benthamiana plants and cross-linking of peptides to vlps. | mimicking and exploiting virus properties and physicochemical and physical characteristics holds promise to provide solutions to some of the world's most pressing challenges. the sheer range and types of viruses coupled with their intriguing properties potentially give endless opportunities for applications in virus-based technologies. viruses have the ability to self- assemble into particles with discrete shape and size, specificity of symmetry, polyvalence, and stable properties under a wide r ... | 2013 | 23439009 |
| electron microscopy of maize rayado fino virus in the internal organs of its leafhopper vector. | discrete groups of 20- to 25-nm isometric particles, considered to represent maize rayado fino virus (rfv), were consistently observed in the ectoderm, fat body, midgut, follicular cells of the ovary, muscle, and salivary glands of rfv-infected dalbulus maidis. the particles commonly occurred within cytoplasmic bodies bound by membranes, some of them resembling lysosomes, and were not found in nonviruliferous insects. these findings are congruent with previous studies on the multiplication of rf ... | 1983 | 6832939 |
| multiplication of maize rayado fino virus in the leafhopper vector dalbulus maidis. | the enzyme-linked immunosorbent assay (elisa) was used to demonstrate the increase in titer of maize rayado fino virus (mrfv) in its leafhopper vector, dalbulus maidis. viral antigen concentration attained a maximum in the body of the insect 25 days after virus acquisition and decreased thereafter. substantial differences in concentration were observed among viruliferous leafhoppers. mrfv was serially passed through 5 successive leafhopper populations. the results provide further evidence of mul ... | 1986 | 3721812 |
| variability of geographically distinct isolates of maize rayado fino virus in latin america. | we have examined the molecular epidemiology of the leafhopper-borne maize rayado fino virus (mrfv) in latin america. the coat protein gene and 3' non-translated region of 14 isolates of mrfv collected from latin america and the united states were sequenced and phylogenetic relationships examined. the nucleotide sequence revealed remarkable conservation, with a sequence similarity of 88-99%. phylogenetic analysis of sequence data obtained from a 633 bp fragment showed that mrfv has diverged into ... | 1997 | 9400964 |
| partial characterization of maize rayado fino virus isolates from ecuador: phylogenetic analysis supports a central american origin of the virus. | maize rayado fino virus (mrfv) infects maize and appears to be restricted to, yet widespread in, the americas. mrfv was previously unreported from ecuador. maize plants exhibiting symptoms of mrfv infection were collected at the santa catalina experiment station in quito, ecuador. rt-pcr reactions were performed on total rna extracted from the symptomatic leaves using primers specific for the capsid protein (cp) gene and 3' non-translated region of mrfv and first strand cdna as a template. nucle ... | 2007 | 17386956 |
| presence of a polya tail at the 3' end of maize rayado fino virus rna. | maize rayado fino virus (mrfv) is distinct from other marafiviruses in that its genome reportedly lacks a poly(a) tail at the 3' terminus. we now show that the mrfv genome is indeed polyadenylated. | 2011 | 21170728 |
| complete genome sequence of switchgrass mosaic virus, a member of a proposed new species in the genus marafivirus. | the complete genome sequence of a virus recently detected in switchgrass (panicum virgatum) was determined and found to be closely related to that of maize rayado fino virus (mrfv), genus marafivirus, family tymoviridae. the genomic rna is 6408 nucleotides long. it contains three predicted open reading frames (orfs 1-3), encoding proteins of 227 kda, 43.9 kda, and 31.5 kda, compared to two orfs (1 and 2) for mrfv. the complete genome shares 76 % sequence identity with mrfv. the nucleotide sequen ... | 2012 | 22661377 |
| mutations in the alpha-helical region of the amino terminus of the maize rayado fino virus capsid protein and cp:rna ratios affect virus-like particle encapsidation of rnas. | viral-based nanoplatforms rely on balancing the delicate array of virus properties to optimally achieve encapsidation of foreign materials with various potential objectives. we investigated the use of maize rayado fino virus (mrfv)-virus-like particles (vlps) as a multifunctional nanoplatform and their potential application as protein cages. mrfv-vlps are composed of two serologically related, carboxy co-terminal coat proteins (cp1 and cp2) which are capable of self-assembling in nicotiana benth ... | 2015 | 25102332 |
| infectious maize rayado fino virus from cloned cdna. | a full-length cdna clone was produced from a u.s. isolate of maize rayado fino virus (mrfv), the type member of the genus marafivirus within the family tymoviridae. infectivity of transcripts derived from cdna clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors dalbulus maidis and graminella nigrifrons that transmit the virus in a persistent-propagative manner. infection of maize plants through vascular puncture inocul ... | 2015 | 25651051 |
| orf43 of maize rayado fino virus is dispensable for systemic infection of maize and transmission by leafhoppers. | maize rayado fino virus (mrfv) possesses an open reading frame (orf43) predicted to encode a 43 kda protein (p43) that has been postulated to be a viral movement protein. using a clone of mrfv (pmrfv-us) from which infectious rna can be produced, point mutations were introduced to either prevent initiation from three potential aug initiation codons near the 5'-end of orf43 or prematurely terminate translation of orf43. inoculation of maize seed via vascular puncture inoculation (vpi) resulted in ... | 2016 | 26837893 |
| overexpression and self-assembly of virus-like particles in nicotiana benthamiana by a single-vector dna replicon system. | based on recent developments, virus-like particles (vlps) are considered to be perfect candidates as nanoplatforms for applications in materials science and medicine. to succeed, mass production of vlps and self-assembly into a correct form in plant systems are key factors. here, we report expression of synthesized coat proteins of the three viruses, brome mosaic virus, cucumber mosaic virus, and maize rayado fino virus, in nicotiana benthamiana and production of self-assembled vlps by transient ... | 2014 | 24965559 |
| maize rayado fino virus virus-like particles expressed in tobacco plants: a new platform for cysteine selective bioconjugation peptide display. | maize rayado fino virus (mrfv) virus-like-particles (vlps) produced in tobacco plants were examined for their ability to serve as a novel platform to which a variety of peptides can be covalently displayed when expressed through a potato virus x (pvx)-based vector. to provide an anchor for chemical modifications, three cys-mrfv-vlps mutants were created by substituting several of the amino acids present on the shell of the wild-type mrfv-vlps with cysteine residues. the mutant designated cys 2-v ... | 2011 | 21963393 |
| complete sequence of fig fleck-associated virus, a novel member of the family tymoviridae. | the complete nucleotide sequence and the genome organization were determined of a novel virus, tentatively named fig fleck-associated virus (ffkav). the viral genome is a positive-sense, single-stranded rna 7046 nucleotides in size excluding the 3'-terminal poly(a) tract, and comprising two open reading frames. orf1 encodes a polypeptide of 2161 amino acids (p240), which contains the signatures of replication-associated proteins and the coat protein cistron (p24) at its 3' end. orf2 codes for a ... | 2011 | 21840352 |
| complete nucleotide sequence and genome organization of olive latent virus 3, a new putative member of the family tymoviridae. | the complete nucleotide sequence and the genome organization were determined of a putative new member of the family tymoviridae, tentatively named olive latent virus 3 (olv-3), recovered in southern italy from a symptomless olive tree. the sequenced ssrna genome comprises 7148 nucleotides excluding the poly(a) tail and contains four open reading frames (orfs). orf1 encodes a polyprotein of 221.6kda in size, containing the conserved signatures of the methyltransferase (mtr), papain-like protease ... | 2010 | 20561953 |
| maize rayado fino virus capsid proteins assemble into virus-like particles in escherichia coli. | maize rayado fino virus (mrfv; genus marafivirus; family tymoviridae) is an isometric plant virus of 30 nm containing two components: empty shells and complete virus particles (encapsidating the 6.3 kb genomic rna). both particles are composed of two serologically related, carboxy co-terminal, coat proteins (cp) of apparent molecular mass 21-22 kda (cp2) and 24-28 kda (cp1) in a molar ratio of 3:1, respectively; cp1 contains a 37 amino acid amino terminal extension of cp2. in our study, expressi ... | 2010 | 19914312 |
| [a method for genetic transformation of maize for resistance to viral diseases]. | a system for the genetic transformation of maize was developed for two costa rican varieties: cr-7 and diamantes 8843, that can allow the subsequent transfer of viral-derived genes in order to confer resistance to the disease caused by maize rayado fino virus (mrfv). the method is based on particle bombardment of organogenic calli derived from shoot tips. on the other hand, the molecular construction prfcp-bar, containing the coat protein gene of mrfv and the marker gene bar, was elaborated. for ... | 2004 | 17361571 |
| molecular characterization of the genome of maize rayado fino virus, the type member of the genus marafivirus. | the complete nucleotide sequence of the single-stranded rna genome of maize rayado fino virus (mrfv), the type member of the genus marafivirus, is 6305 nucleotides (nts) in length and contains two putative open reading frames (orfs). the largest orf (nt 97-6180) encodes a polyprotein of 224 kda with sequence similarities at its n-terminus to the replication-associated proteins of other viruses with positive-strand rna genomes and to the papainlike protease domain found in tymoviruses. the c-term ... | 2001 | 11289816 |
| the two capsid proteins of maize rayado fino virus contain common peptide sequences. | virions of maize rayado fino virus (mrfv) were purified and two major capsid proteins (ca. mr 29,000 and 22,000) were resolved by sds-page. when the two major capsid proteins were isolated from gels and compared by one-dimensional peptide mapping after digestion with staphylococcus aureus v-8 protease, indistinguishable peptide maps were obtained, suggesting that these two proteins contain common peptide sequences. some preparations also showed minor protein components that were intermediate bet ... | 1986 | 3721810 |