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synthesis of collagenase by the phytopathogenic bacterium corynebacterium rathayii.the collagenase-producing bacterium tentatively called empedobacter collagenolyticum was recently identified as corynebacterium rathayii. production of collagenase was studied in different media containing peptones or peptides of known sequences. a hexapeptide, pro-ala-gly-pro-pro-gly, is an inducer almost as good as collagen.19901962771
empedopeptin (bmy-28117), a new depsipeptide antibiotic. i. production, isolation and properties.empedopeptin is a new antibiotic produced by empedobacter haloabium nov. sp. (atcc 31962). it is a water-soluble depsipeptide antibiotic containing eight amino acid residues and a c14-fatty acid moiety in the molecule. although structurally unrelated, empedopeptin and vancomycin have similar antimicrobial spectra against aerobic and anaerobic gram-positive bacteria including antibiotic-resistant strains. empedopeptin is highly active in vivo in mice against systemic infections of staphylococcus ...19846501108
[purification and partial characterization of an extracellular aminopeptidase of a collagenolytic bacterium: empedobacter collagenolyticum].an aminopeptidase was purified from the culture filtrates of a collagenolytic bacterium empedobacter collagenolyticum. purification of this enzyme was accomplished by ammonium sulphate precipitation, gel filtration on sephadex-g200 and chromatography on deae-sephacel. the purified enzyme seemed homogeneous on polyacrylamide gel electrophoresis. the molecular weight of the enzyme was about 100,000 daltons as determined by gel filtration on sephadex-g200 but it was only 33,000 daltons by disc gel ...19826819795
rearing stable fly larvae (diptera: muscidae) on an egg yolk medium.the growth and survival of stomoxys calcitrans (l.) larvae on egg yolk medium inoculated with bacteria isolated from a colony of stable flies was evaluated. five species of bacteria--acinetobacter sp., aeromonas sp., empedobacter breve (holmes & owen), flavobacterium odoratum stutzer, and serratia marcescens bizio--were identified according to fatty acid profiles using a microbial identification system. larvae failed to develop on uninoculated plates, confirming that bacteria are required to com ...199910337112
psychrobacters and related bacteria in freshwater fish.three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ...200010716558
a polyphasic taxonomic study of chryseobacterium strains isolated from dairy sources.a polyphasic taxonomic study, employing protein electrophoresis (sds-page), gas chromatographic analysis of cellular fatty acids (fame), mol% g+c determination and dna-dna hybridizations, was undertaken on 103 dairy isolates shown to belong to chryseobacterium. reference strains of the chryseobacterium species, cdc group iib and embedobacter brevis were included. sds-page analysis yielded good differentiation between the investigated species. about half of the strains could be clustered into nin ...199910794147
polyamine distribution profiles in newly validated genera and species within the flavobacterium-flexibacter-cytophaga-sphingobacterium complex.cellular polyamines of 58 strains belonging to the flavobacterium-flexibacter-cytophaga-sphingobacterium complex were analysed by hplc. homospermidine was found in all species of flavobacterium, chryseobacterium, empedobacter, myroides, cellulophaga, salegentibacter, psychroserpens and gelidibacter of the family flavobacteriaceae. flavobacterium ferrugineum located outside of this family also contained homospermidine. cytophaga fermentans and c. xylanolytica belonging to the family bacteroidacea ...200111548199
outbreak of empedobacter brevis endophthalmitis after cataract extraction.we present a series of patients who were operated upon on the same day by the same surgeon. all of those patients developed postoperative endophthalmitis due to empedobacter brevis - a bacterium hitherto unknown in ophthalmologic literature.200211981643
ebr-1, a novel ambler subclass b1 beta-lactamase from empedobacter brevis.empedobacter brevis (formerly designated flavobacterium breve) is a gram-negative aerobe involved in nosocomial infections. the ambler class b beta-lactamase gene bla(ebr-1) was cloned and expressed in escherichia coli from e. brevis clinical strain ass-1, which had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. purified beta-lactamase ebr-1 hydrolyzed penicillins, cephalosporins, and carbapenems efficiently but not aztreonam. kinetic parameters of ebr-1 were similar ...200212234848
novel growth media for rearing larval horn flies, haematobia irritans (diptera: muscidae).experiments were conducted to develop an agar-based medium for rearing immature horn flies, hematobia irritans (l.). larval survival was determined on sterilized manure inoculated with pure and mixed cultures of acinetobacter sp., bacillus pumilus meyer & gottheil, comamonas acidovorans den dooren de jong, pseudomonas mendocina palleroni, flavobacterium sp. and empedobacter breve (holmes & owen). rearing larvae on mixed cultures enhanced pupal weight but not survival. horn fly larvae failed to s ...200312597649
[research on strain of empedobacter: empedobacter uliginosum, var. natricis]. 195813617708
[study of a strain of empedobacter isolated from sputum]. 195913804501
planktonic replication is essential for biofilm formation by legionella pneumophila in a complex medium under static and dynamic flow conditions.legionella pneumophila persists for a long time in aquatic habitats, where the bacteria associate with biofilms and replicate within protozoan predators. while l. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clear whether the bacteria form or replicate within biofilms in the absence of protozoa. in this study, we analyzed surface adherence of and biofilm formation by l. pneumophila in a rich medium that supported axenic replication. biofilm formation by t ...200616597995
inhibitory effect of oxalic acid on bacterial spoilage of raw chilled chicken.oxalic acid was evaluated as a treatment for reducing populations of naturally occurring microorganisms on raw chicken. raw chicken breasts were dipped in solutions of oxalic acid (0, 0.5, 1.0, 1.5, and 2.0%, wt/vol) for 10, 20, and 30 min, individually packed in oxygen-permeable polyethylene bags, and stored at 4 degrees c. total plate counts of aerobic bacteria and populations of pseudomonas spp. and enterobacteriaceae on breasts were determined before treatment and after storage for 1, 3, 7, ...200616924917
description of wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera chryseobacterium and empedobacter.a total of 26 isolates of non-fermenting, gram-negative rods, obtained between 1980 and 2004 by various clinical laboratories in belgium, with phenotypic characteristics resembling those of members of the genera chryseobacterium and empedobacter (indole-positive) and a biochemical profile resembling that of cdc group ii-h, but urease-positive, were collected at the université catholique de louvain microbiology laboratory, belgium. the 16s rrna gene sequences were determined for most of the isola ...200617012555
spectrum of activity, mutation rates, synergistic interactions, and the effects of ph and serum proteins for fusidic acid (cem-102).fusidic acid (cem-102) is a steroidal antimicrobial agent with focused gram-positive activity that acts by preventing bacterial protein synthesis via interacting with elongation factor g. a collection of 114 wild-type isolates (> 80 species) was used to define the contemporary limits of fusidic acid spectrum against gram-positive and gram-negative species. reference broth microdilution and anaerobic agar dilution methods were performed. modifications of standardized test methods included adding ...201020159376
depsides and other polar constituents from origanum dictamnus l. and their in vitro antimicrobial activity in clinical strains.phytochemical investigation of the polar extracts of the aerial parts of origanum dictamnus afforded 15 secondary metabolites. one new depside was isolated, to which the trivial name salvianolic acid p (1) was given, in addition to the known depsides rosmarinic acid (2) and rosmarinic acid methyl ester (3), as well as two monoterpenes, thymoquinone (4) and thymoquinol 2-o-beta-glucopyranoside (5); two simple phenolic acids, oresbiusin a (6) and e-caffeic acid (7); six flavonoids, namely, apigeni ...201020423097
[a case of anaphylactoid purpura suggested to empedobacter (flavobacterium) brevis infection concerned].the patient was an 83 year-old woman with purpura on her right foot and on both crura starting from the end of november 2005. first, she took medical advice from a nearby doctor, but it did not improve. since blisters were formed besides purpura, she visited nagoya city midori municipal hospital. when she visited, she had many purpura on her both legs, her right lower crus and her right foot was swelling with redness, blisters and erosion. histopathological finding showed leukocytoclastic vascul ...201020502105
Gene cloning and characterization of a-amino acid ester acyl transferase in Empedobacter brevis ATCC14234 and Sphingobacterium siyangensis AJ2458.The gene encoding a-amino acid ester acyl transferase (AET), the enzyme that catalyzes the peptide-forming reaction from amino acid methyl esters and amino acids, was cloned from Empedobacter brevis ATCC14234 and Sphingobacterium siyangensis AJ2458 and expressed in Escherichia coli. This is the first report on the aet gene. It encodes a polypeptide composed of 616 (ATCC14234) and 619 (AJ2458) amino acids residues. The V(max) values of these recombinant enzymes during the catalysis of L-alanyl-L- ...201122056425
[purification and study of some properties of a collagenase produced by empedobacter collagenolyticum].a collagenase from empedobacter collagenolyticum was extracted from the culture medium of the bacteria. the complete purification of the enzyme was achieved by successive ammonium sulfate precipitation. sephadex g 200 gel filtration and deae cellulose chromatography. this collagenase is active on insoluble collagen, and on the synthetic peptide pz-pro-leu-gly-pro-d-arg. its optimum activity was at 30 degrees c and at ph 7.6. a strong inhibition was observed with chelating agents such as o-phenan ...19826279175
[inventory of proteolytic activity of a new collagenolytic bacteria empedobacter collagenolyticum].during the growth of empedobacter collagenolyticum on a medium with gelatin, only one proteinase, a collagenase, was excreted in the culture medium. no other proteolytic activity was detected in the extracellular medium or in acellular extracts. the other proteases of this bacteria are principally intracellular peptidases. by electrophoresis of an acellular extract five peptidases were separated; they were aminopeptidases and dipeptidases. some of them exhibited a specificity towards peptides wi ...19826279174
a novel and efficient enzymatic method for the production of peptides from unprotected starting materials.we report herein the development of a novel and efficient enzymatic method for the production of oligopeptides. this newly discovered method is a simple, cost-effective process, using unprotected amino acids as substrates in an aqueous solution and producing peptides in high yield. the target of our initial screen was l-alanyl-l-glutamine, a dipeptide of significant industrial interest by virtue of its widespread use in infusion therapy. by means of the screening of microorganisms that can catal ...200515607239
the incidence of weeksella- and bergeyella-like bacteria in the food environment.eighty-five catalase- and oxidase-positive gram-negative rods and cocci susceptible to penicillin g were isolated from a variety of food sources. the phenotypic relationships of these isolates with reference cultures of bergeyella-like, chryseobacterium, empedobacter, myroides, moraxella, sphingobacterium and weeksella-like strains were examined by numerical taxonomy. seventy-three isolates were recovered in five groups; 80% of the isolates clustered in groups 1, 2 and 3 and produced indole, bea ...19989721639
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