| sulfobacins a and b, novel von willebrand factor receptor antagonists. i. production, isolation, characterization and biological activities. | sulfobacins a and b, novel von willebrand factor (vwf) receptor antagonists, have been isolated from the culture broth of chryseobacterium sp. (flavobacterium sp.) nr 2993 by ethyl acetate extraction, and by sephadex lh-20 and silica gel column chromatographies. the physico-chemical properties of the sulfobacins indicate that their structures are completely different from that of aurintricarboxylic acid, the one known vwf receptor antagonist. sulfobacins a and b inhibit the binding of vwf to its ... | 1995 | 7592056 |
| sulfobacins a and b, novel von willebrand factor receptor antagonists. ii. structural elucidation. | sulfobacins a and b are novel von willebrand factor (vwf) receptor antagonists produced by chryseobacterium sp. nr 2993. the structures of sulfobacins a and b have been determined to be (2r,3r)-3-hydroxy-2-[(r)-3-hydroxy-15-methylhexadecanamido]-15- methylhexadecanesulfonic acid and (2r,3r)-3-hydroxy-15-methyl-2-[13-methyltetradecanamido]- hexadecanesulfonic acid, respectively, by various 2d nmr experiments and by methanolysis. the absolute configurations of the sulfobacins were determined by a ... | 1995 | 7592057 |
| ribotyping of chryseobacterium meningosepticum: its use as an epidemiological tool and its correlation with serovars. | chryseobacterium meningosepticum (basonym, flavobacterium meningosepticum king 1959) is associated with neonatal meningitis and is isolated from normal and immunocompromised adults. aaf-labelled escherichia coli 16 + 23s rrna was used as a probe for ribotype analysis of 92 clinical isolates from tracheal exsudate, blood culture, cerebrospinal fluid, urine and pus. the 92 isolates belonged to the 15 described serovars of c. meningosepticum, and included 21 strains isolated during an outbreak in a ... | 1996 | 8763627 |
| chryseobacterium meningosepticum: an emerging pathogen among immunocompromised adults. report of 6 cases and literature review. | chryseobacterium meningosepticum is a ubiquitous gram-negative bacillus historically associated with meningitis in premature neonates. we report 15 positive cultures and 6 cases of infection among immunocompromised adults at our institution over a 10-year period and review the english-language literature on c. meningosepticum. excluding the present series, there are 308 reports of positive cultures in the literature, of which 59% were determined to represent true infections. sixty-five percent o ... | 1997 | 9064486 |
| phylogenetic position of riemerella anatipestifer based on 16s rrna gene sequences. | riemerella anatipestifer, the causative agent of septicemia anserum exsudativa (also called new duckling disease), belongs to the family flavobacteriaceae of gram-negative bacteria. we determined the dna sequences of the rrs genes encoding the 16s rrnas of four r. anatipestifer strains by directly sequencing pcr-amplified rrs genes. a sequence similarity analysis confirmed the phylogenetic position of r. anatipestifer in the family flavobacteriaceae in rrna superfamily v and allowed fine mapping ... | 1997 | 9103649 |
| the filamentous morphotype eikelboom type 1863 is not a single genetic entity. | five isolates of a filamentous bacterial morphotype with the distinctive diagnostic microscopic features of eikelboom type 1863 were obtained from activated sludge sewage treatment plants in victoria, australia. on the basis of phenotypic evidence and 16s rdna sequence data, these isolates proved to be polyphyletic. two (ben 06 and ben 06c) are from the chryseobacterium subgroup which is in the cytophaga group, subdivision i of the flexibacter-cytophaga-bacteroides phylum. two (ben 56 and ben 59 ... | 1997 | 9134715 |
| comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods. | in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ... | 1997 | 9210669 |
| immunization with bacterial antigens: flavobacterium and flexibacter infections. | seven bacterial species belonging to the flavobacterium-cytophaga group are currently considered to be pathogenic for fish. because they were only recently described and/or because the disease they provoke has little economic significance, no study has been performed concerning immunization against chryseobacterium scophthalmum, flavobacterium johnsoniae and flexibacter ovolyticus. immunization with flexibacter maritimus has not been investigated, and fish surviving a natural infection appear to ... | 1997 | 9270847 |
| increasing incidence of nosocomial chryseobacterium indologenes infections in taiwan. | to understand the clinical features, antimicrobial therapy, and epidemiology of chryseobacterium indologenes infections, the medical records of 36 patients with nosocomial chryseobacterium indologenes infections seen over a three-year period at national taiwan university hospital were reviewed. the 36 isolates recovered from these patients were studied by molecular typing and determination of antimicrobial susceptibility patterns. nine patients had underlying neoplastic diseases, seven had diabe ... | 1997 | 9323467 |
| susceptibilities of chryseobacterium indologenes and chryseobacterium meningosepticum to cefepime and cefpirome. | in vitro activities of cefepime and cefpirome against 96 isolates of chryseobacterium indologenes and 21 of c. meningosepticum were determined by the agar dilution method. overall, cefepime was more active than cefpirome against c. indologenes (mic at which 50% of the isolates were inhibited [mic50] and mic90, 4 and 16 microg/ml, respectively, for cefepime and 8 and 128 microg/ml, respectively, for cefpirome). both agents had poor potency against c. meningosepticum (mic50 and mic90, 64 and >256 ... | 1997 | 9399547 |
| reappraisal of the antimicrobial susceptibilities of chryseobacterium and flavobacterium species and methods for reliable susceptibility testing. | several flavobacterium species, comprising a heterogeneous group of gram-negative bacilli that are capable of causing opportunistic infections in humans, have recently been reclassified as chryseobacterium or myroides species. intrinsically resistant to a number of antibiotics, these organisms have been reported to be susceptible to vancomycin and certain other drugs that are normally active against gram-positive bacteria. by using the national committee for clinical laboratory standards (nccls) ... | 1997 | 9420049 |
| characterization and sequence of the chryseobacterium (flavobacterium) meningosepticum carbapenemase: a new molecular class b beta-lactamase showing a broad substrate profile. | the metallo-beta-lactamase produced by chryseobacterium (formerly flavobacterium) meningosepticum, which is the flavobacterial species of greatest clinical relevance, was purified and characterized. the enzyme, named blab, contains a polypeptide with an apparent mr of 26000, and has a pi of 8.5. it hydrolyses penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate, a mechanism-based inactivator of active-site serine beta-lactamases. the enzyme was inhibited by ... | 1998 | 9576862 |
| detection of acid production from carbohydrates by riemerella anatipestifer and related organisms using the buffered single substrate test. | one hundred and twenty-one riemerella anatipestifer field strains from wild birds, domesticated poultry and pigs were examined for their ability to produce acid from carbohydrates by using conventional biochemical and buffered single substrate (bss) test methods. the type strains of the species r. anatipestifer and taxometrically related genera chryseobacterium and bergeyella were included in the study. in contrast to 10 indole-positive r. anatipestifer variant strains, only a few of the 111 typ ... | 1998 | 9646458 |
| the incidence of weeksella- and bergeyella-like bacteria in the food environment. | eighty-five catalase- and oxidase-positive gram-negative rods and cocci susceptible to penicillin g were isolated from a variety of food sources. the phenotypic relationships of these isolates with reference cultures of bergeyella-like, chryseobacterium, empedobacter, myroides, moraxella, sphingobacterium and weeksella-like strains were examined by numerical taxonomy. seventy-three isolates were recovered in five groups; 80% of the isolates clustered in groups 1, 2 and 3 and produced indole, bea ... | 1998 | 9721639 |
| [evaluation of antibacterial activities of various antibiotics against glucose non-fermentative gram-negative rods other than pseudomonas aeruginosa]. | mics of piperacillin, sulbactam/cefoperazone, minocycline (mino), gentamicin, amikacin, flomoxef, ceftazidime, cefozopran, cefsulodin and imipenem were determined, against 189 clinical isolated strains of glucose non-fermentative gram-negative rods (nfgnr; acinetobacter baumannii (44), alcaligenes faecalis (5), alcaligenes xylosoxidans (25), burkholderia cepacia (12), chryseobacterium indologenes (23), chryseobacterium meningosepticum (9), pseudomonas fluorescens (8), pseudomonas putida (12), st ... | 1998 | 9755431 |
| community-acquired chryseobacterium meningosepticum pneumonia and sepsis in a previously healthy child. | | 1998 | 9892546 |
| molecular characterization of a carbapenem-hydrolyzing beta-lactamase from chryseobacterium (flavobacterium) indologenes. | chryseobacterium (flavobacterium) indologenes 001 clinical strain was resistant to several beta-lactam classes including carbapenems. shotgun cloning experiments of sau3ai restricted genomic dna of c. indologenes 001 into pbkcmv cloning vector followed by transformation into escherichia coli dh10b gave one recombinant plasmid possessing a 4.2-kb dna insert. it encoded a pi 7.2 beta-lactamase of 239 amino acids (ind-1) which is a metallo-enzyme with a broad spectrum beta-lactam hydrolysis profile ... | 1999 | 10077836 |
| purification and characterization of a metalloprotease from chryseobacterium indologenes ix9a and determination of the amino acid specificity with electrospray mass spectrometry. | the heat-stable protease from chryseobacterium indologenes ix9a was purified to homogeneity using immobilized metal affinity chromatography. the enzyme was characterized as a metalloprotease with an approximate relative molecular mass of 24,000, a ph optimum of 6.5, and a high temperature optimum (50 degrees c). the metal chelator edta and the zn2+-specific chelator 1,10-phenanthroline were identified as inhibitors and atomic absorption analysis showed that the enzyme contained ca2+ and zn2+. th ... | 1999 | 10092488 |
| cloning and characterization of blavim, a new integron-borne metallo-beta-lactamase gene from a pseudomonas aeruginosa clinical isolate. | production of a metallo-beta-lactamase activity was detected in a carbapenem-resistant pseudomonas aeruginosa clinical isolate (isolate vr-143/97) from an italian inpatient at the verona university hospital (northern italy). the metallo-beta-lactamase determinant was isolated from a genomic library of vr-143/97, constructed in an escherichia coli plasmid vector, by screening for clones with reduced susceptibility to imipenem. sequencing of the cloned gene revealed that it encoded a new class b b ... | 1999 | 10390207 |
| cloning of a chryseobacterium (flavobacterium) meningosepticum chromosomal gene (blaa(cme)) encoding an extended-spectrum class a beta-lactamase related to the bacteroides cephalosporinases and the veb-1 and per beta-lactamases. | in addition to the blab metallo-beta-lactamase, chryseobacterium (flavobacterium) meningosepticum ccug 4310 (nctc 10585) constitutively produces a 31-kda active-site serine beta-lactamase, named cme-1, with an alkaline isoelectric ph. the blaa(cme) gene that encodes the latter enzyme was isolated from a genomic library constructed in the escherichia coli plasmid vector pacyc184 by screening for cefuroxime-resistant clones. sequence analysis revealed that the cme-1 enzyme is a new class a beta-la ... | 1999 | 10471563 |
| chryseobacterium meningosepticum bacteremia secondary to central intravenous line-related infection. | | 1999 | 10586158 |
| genetic-biochemical analysis and distribution of the ambler class a beta-lactamase cme-2, responsible for extended-spectrum cephalosporin resistance in chryseobacterium (flavobacterium) meningosepticum. | in vitro synergy between extended-spectrum cephalosporins and either clavulanic acid or cefoxitin was found for chryseobacterium meningosepticum isolates during a double-disk assay on an agar plate. an extended-spectrum beta-lactamase (esbl) gene from a c. meningosepticum clinical isolate was cloned and expressed in escherichia coli dh10b. its protein conferred resistance to most beta-lactams including extended-spectrum cephalosporins but not to cephamycins or to imipenem. its activity was stron ... | 2000 | 10602714 |
| specific proteolysis of human plasminogen by a 24 kda endopeptidase from a novel chryseobacterium sp. | a novel single polypeptide endopeptidase of 24 kda (24k-endopeptidase) was purified with a yield of 300-400 microg/l from conditioned medium of a bacterial strain which was identified as a new species in the genus chryseobacterium sp. on the basis of its 16s rdna sequence and dna:dna hybridizations. the nh(2)-terminal amino acid sequence (val-ala-thr-pro-asn-leu-glu-.) was not found in the availabe databases. the 24k-endopeptidase specifically hydrolyzed the ser(441)-val(442) peptide bond in hum ... | 2000 | 10631010 |
| chryseobacterium meningosepticum (flavobacterium meningosepticum)--a report of five cases in a local hospital. | chrysobacterium meningosepticum (flavobacterium meningosepticum) is a known cause of meningitis in premature and newborn infants. infection due to this organism in adults is uncommon. we report 5 cases of chrysobacterium meningosepticum in adult patients. most of these patients were elderly and had underlying co-morbidities. | 1999 | 10672403 |
| evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli. | the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ... | 2000 | 10699007 |
| psychrobacters and related bacteria in freshwater fish. | three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ... | 2000 | 10716558 |
| characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france. | pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ... | 2000 | 10722487 |
| tla-1: a new plasmid-mediated extended-spectrum beta-lactamase from escherichia coli. | escherichia coli r170, isolated from the urine of an infected patient, was resistant to expanded-spectrum cephalosporins, aztreonam, ciprofloxacin, and ofloxacin but was susceptible to amikacin, cefotetan, and imipenem. this particular strain contained three different plasmids that encoded two beta-lactamases with pis of 7.0 and 9.0. resistance to cefotaxime, ceftazidime, aztreonam, trimethoprim, and sulfamethoxazole was transferred by conjugation from e. coli r170 to e. coli j53-2. the transfer ... | 2000 | 10722503 |
| a polyphasic taxonomic study of chryseobacterium strains isolated from dairy sources. | a polyphasic taxonomic study, employing protein electrophoresis (sds-page), gas chromatographic analysis of cellular fatty acids (fame), mol% g+c determination and dna-dna hybridizations, was undertaken on 103 dairy isolates shown to belong to chryseobacterium. reference strains of the chryseobacterium species, cdc group iib and embedobacter brevis were included. sds-page analysis yielded good differentiation between the investigated species. about half of the strains could be clustered into nin ... | 1999 | 10794147 |
| carbapenemases of chryseobacterium (flavobacterium) meningosepticum: distribution of blab and characterization of a novel metallo-beta-lactamase gene, blab3, in the type strain, nctc 10016. | genes encoding carbapenemases in 15 reference strains of chryseobacterium (flavobacterium) meningosepticum from the united kingdom national collection of type cultures and in one recent clinical isolate were investigated. all the strains hydrolyzed imipenem, but their levels of resistance to carbapenems varied, with imipenem and meropenem mics ranging from 2 to >32 microg/ml. the blab gene, which encodes a molecular-class b carbapenemase, was detected in only six reference strains and in clinica ... | 2000 | 10817691 |
| molecular and biochemical heterogeneity of class b carbapenem-hydrolyzing beta-lactamases in chryseobacterium meningosepticum. | although the carbapenem-hydrolyzing beta-lactamase (chbetal) blab-1 is known to be in chryseobacterium meningosepticum nctc 10585, a second chbetal gene, bla(gob-1), was cloned from another c. meningosepticum clinical isolate (pint). the g+c content of bla(gob-1) (36%) indicated the likely chromosomal origin of this gene. its expression in escherichia coli dh10b yields a mature chbetal with a pi of 8.7 and a relative molecular mass of 28.2 kda. in e. coli, gob-1 conferred resistance to narrow-sp ... | 2000 | 10858348 |
| characteristics of neonatal bacterial meningitis in a teaching hospital in taiwan from 1984-1997. | during the period from 1984 to 1997, 85 bacterial meningitis neonates with positive cerebrospinal fluid cultures were treated. the ages of these patients ranged from 1 to 28 days. the male to female ratio was 1.7 to 1. the most common causative agent was group b beta-hemolytic streptococci (gbs, 31.8%), followed by escherichia coli (20%), proteus mirabilis (7.1%), enterobacter cloacae (5.9%), other streptococci excluding streptococcus pneumoniae (5.9%), chryseobacterium meningosepticum (5.9%), e ... | 2000 | 10917879 |
| a novel protein-deamidating enzyme from chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. | a novel protein-deamidating enzyme, which has potential for industrial applications, was purified from the culture supernatant of chryseobacterium proteolyticum strain 9670(t) isolated from rice field soil in tsukuba, japan. the deamidating activities on carboxybenzoxy (cbz)-gln-gly and caseins and protease activity were produced synchronously by the isolate. both deamidating activities were eluted as identical peaks separated from several proteases by phenyl-sepharose chromatography of the cult ... | 2000 | 10919788 |
| [non-fermenting gram-negative bacilli: distribution in clinical specimens and antimicrobial susceptibility]. | in the present work was studied the prevalence, distribution in clinical specimens, and antimicrobial susceptibility of non-fermentative gram-negative bacilli (nfgnb) from patients attended at hospital "angela i. de llano" (corrientes, argentina). a total of 125 strains of nfgnb were recovered from various clinical specimens from july, 1997 to december, 1998. isolates were identified by classical biochemical tests. drug sensitivity was performed by standard methods with cefotaxime (ctx), ceftazi ... | 1999 | 10932769 |
| atypical chryseobacterium meningosepticum and meningitis and sepsis in newborns and the immunocompromised, taiwan. | from 1996 to 1999, 17 culture-documented systemic infections due to novel, atypical strains of chryseobacterium meningosepticum occurred in two newborns and 15 immunocompromised patients in a medical center in taiwan. all clinical isolates, which were initially misidentified as aeromonas salmonicida by an automated bacterial identification system, were resistant to a number of antimicrobial agents. the isolates were characterized as atypical strains of c. meningosepticum by complete biochemical ... | 2000 | 10998378 |
| genetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from chryseobacterium (flavobacterium) indologenes. | the class b carbapenem-hydrolyzing beta-lactamase ind-1 has been characterized for chryseobacterium indologenes strain 001. with internal primers for the bla gene for ind-1 (bla(ind-1)) and an internal bla(ind-1) probe, pcr amplifications failed, while hybridization results were positive when dna from another c. indologenes isolate, strain cip101026, was used as a template. thus, a bla(ind)-related gene was cloned from this c. indologenes reference strain. sequencing of the insert of a recombina ... | 2000 | 11036018 |
| waterborne nosocomial infections. | waterborne pathogens cause infections in health-care facilities. despite guidelines addressing these pathogens, outbreaks and pseudo-outbreaks continue to occur. we reviewed recent reports of infections caused by pseudomonas aeruginosa, stenotrophomonas maltophilia, chryseobacterium species, nontuberculous mycobacteria, and legionella species. mycobacterium avium complex (mac) infection in hiv patients has been linked to hospital water distribution systems; molecular subtyping showed that mac is ... | 2000 | 11095897 |
| metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum. | eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ... | 2001 | 11181369 |
| chryseobacterium in burn wounds. | chryseobacteria are gram negative organisms, formerly known as flavobacteria, which rarely cause infections of burn wounds. this article documents three cases of chryseobacterium infection in burn wounds and adds to the other two cases that have been reported in english literature. two patients died, with one of the deaths linked to a chryseobacteria bacteraemia. in two patients, there was an associated history of first aid treatment with untreated water. patients whose burn wounds are suspected ... | 2001 | 11226658 |
| protein-glutaminase from chryseobacterium proteolyticum, an enzyme that deamidates glutaminyl residues in proteins. purification, characterization and gene cloning. | a novel protein-deamidating enzyme was purified to homogeneity from chryseobacterium proteolyticum and the gene encoding it was cloned. the enzyme is a monomer with a pi of 10.0, a measured m(r) of approximately 20,000 and a calculated m(r) of 19,860. extensive comparison with streptoverticillium transglutaminase showed that the protein-deamidating enzyme lacked transglutaminase activity in terms of hydroxamate-formation between benzyloxycarbonyl-gln-gly and hydroxylamine, or monodansylcadaverin ... | 2001 | 11231294 |
| chryseobacterium (flavobacterium) meningosepticum outbreak associated with colonization of water taps in a neonatal intensive care unit. | from september 1994 to may 1996, a strain of multi-resistant chryseobacterium (flavobacterium) meningosepticum was isolated from eight neonates on a neonatal intensive care unit. the strain was resistant to ampicillin, ceftazidime, imipenem, gentamicin, ciprofloxacin and trimethoprim-sulphamethoxazole, susceptible to piperacillin and amikacin, and had variable susceptibility to rifampicin and vancomycin. two neonates were infected (one had pneumonia and one septicaemia and meningitis); the remai ... | 2001 | 11247678 |
| high protease activity of chryseobacterium indologenes isolates associated with invasive infection. | in order to understand virulence factors of chryseobacterium indologenes isolates associated with invasive infection, enzymatic activities and cellular fatty acid profiles of 42 isolates recovered at national taiwan university hospital from january 1994 to december 1996 were studied. among them, 12 blood isolates were considered as invasive and 30 (recovered from urine, sputa, infected burn wounds, and catheter tips) were noninvasive. all isolates showed strong activities of alkaline phosphatase ... | 2000 | 11269365 |
| isolation and characterization of a chryseobacterium strain from the gut of the american cockroach, periplaneta americana. | a 16s rdna sequence cloned directly from whole-gut microbiota of the american cockroach, periplaneta americana, indicated the presence of a member of the bacteroides/flavobacterium group most closely related to the genus flavobacterium. in an attempt to confirm this finding, we isolated a yellow-pigmented bacterium (strain fr2) from the hindgut of this insect. strain fr2 was phylogentically and phenotypically most similar to species of flavobacterium and related bacteria, namely chryseobacterium ... | 2001 | 11382221 |
| recurrent bacteremia by chryseobacterium indologenes in an oncology patient with a totally implanted intravascular device. | chryseobacterium indologenes was isolated from the blood cultures of an oncological patient with a totally implantable device. because a catheter-related infection was suspected, the port-a-cath was removed after a 10-day course of piperacillin-tazobactam. differences in susceptibility may exist if either the criteria for either pseudomonas or enterobacteriaceae are used. | 2001 | 11531991 |
| cellulitis and bacteraemia due to chryseobacterium indologenes. | | 2001 | 11545560 |
| polyamine distribution profiles in newly validated genera and species within the flavobacterium-flexibacter-cytophaga-sphingobacterium complex. | cellular polyamines of 58 strains belonging to the flavobacterium-flexibacter-cytophaga-sphingobacterium complex were analysed by hplc. homospermidine was found in all species of flavobacterium, chryseobacterium, empedobacter, myroides, cellulophaga, salegentibacter, psychroserpens and gelidibacter of the family flavobacteriaceae. flavobacterium ferrugineum located outside of this family also contained homospermidine. cytophaga fermentans and c. xylanolytica belonging to the family bacteroidacea ... | 2001 | 11548199 |
| changes in populations of rhizosphere bacteria associated with take-all disease of wheat. | take-all, caused by gaeumannomyces graminis var. tritici, is one of the most important fungal diseases of wheat worldwide. knowing that microbe-based suppression of the disease occurs in monoculture wheat fields following severe outbreaks of take-all, we analyzed the changes in rhizosphere bacterial communities following infection by the take-all pathogen. several bacterial populations were more abundant on diseased plants than on healthy plants, as indicated by higher counts on a pseudomonas-se ... | 2001 | 11571137 |
| the chryseobacterium meningosepticum pafa enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases? | chryseobacterium meningosepticum is an aerobic gram-negative rod widely distributed in natural environments. unlike many bacteria, it produces a phosphate-irrepressible periplasmic alkaline phosphatase (ap). this work describes cloning of the gene encoding that enzyme from c. meningosepticum ccug 4310 (nctc 10585), and preliminary characterization of its product. the gene, named pafa, encodes a protein (pafa) of 546 amino acids with a calculated molecular mass of the mature peptide of 58682 da. ... | 2001 | 11577161 |
| [a pilot study on the value of duplex polymerase chain reaction method in early diagnosis of legionella pneumonia]. | to investigate the value of duplex polymerase chain reaction (dpcr) in early diagnosis of legionella pneumonia by detecting legionella dna in sputum and bronchoalveolar lavage fluid(balf). | 2001 | 11798569 |
| identification of the general acid/base catalyst of a family 3 beta-glucosidase from flavobacterium meningosepticum. | beta-glucosidase from flavobacterium meningosepticum (fbgl) (also known as chryseobacterium meningosepticum) has been classified as a member of the family 3 glycohydrolases. it is a retaining enzyme involving a two-step, double-displacement mechanism. d247 was shown to function as the nucleophile of the enzymatic reaction [li, y.-k., chir, j., and chen, f.-y. (2001) biochem. j. 355, 835-840]. however, the general acid/base catalyst of this enzyme and of all other family 3 glycohydrolases has not ... | 2002 | 11851422 |
| molecular and biochemical characterization of ambler class a extended-spectrum beta-lactamase cga-1 from chryseobacterium gleum. | antibiotic susceptibility testing by disk diffusion of a chryseobacterium gleum isolate, strain cip 103039, showed a typical synergy image between clavulanic acid and expanded-spectrum cephalosporins. shotgun cloning gave a recombinant plasmid in escherichia coli that produced a beta-lactamase, cga-1, with a pi value of 8.9 that conferred resistance to most penicillins (except ureidopenicillins) and narrow-spectrum cephalosporins and an intermediate susceptibility to expanded-spectrum cephalospo ... | 2002 | 11897576 |
| substrate-activated zinc binding of metallo-beta -lactamases: physiological importance of mononuclear enzymes. | we have investigated the influence of substrate binding on the zinc ion affinity of representatives from the three metallo-beta-lactamase subclasses, b1 (bcii from bacillus cereus and blab from chryseobacterium meningosepticum), b2 (cpha from aeromonas hydrophila), and b3 (l1 from stenotrophomonas maltophilia). by competition experiments with metal-free apoenzymes and chromophoric zinc chelators or edta, we determined the dissociation constants in the absence and presence of substrates. for the ... | 2002 | 11967267 |
| overproduction and biochemical characterization of the chryseobacterium meningosepticum blab metallo-beta-lactamase. | the blab metallo-beta-lactamase of chryseobacterium meningosepticum ccug4310 was overproduced in escherichia coli by means of a t7 promoter-based expression system. the overproducing system, scaled up in a 15-liter fermentor, yielded approximately 10 mg of blab protein per liter, mostly released in the culture supernatant. the enzyme was purified by two ion-exchange chromatographic steps with an overall yield of 66%. analysis of the kinetic parameters revealed efficient activities (k(cat)/k(m) r ... | 2002 | 12019109 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| septic arthritis caused by chryseobacterium meningosepticum in an immunocompetent male. | this is the first report of septic arthritis of the knee caused by chryseobacterium meningosepticum. the infection was associated with a penetrating injury to the joint. the patient was cured by flushing of the joint and antibiotic treatment. | 2002 | 12064694 |
| inhibition of bacterial alpha-glucosidases by castanospermine in pure cultures and activated sludge. | castanospermine (cast) is a known and potent inhibitor of various alpha-glucosidases in eukaryotes. in this work, we elucidated whether cast could also be used for determining bacterial alpha-glucosidase activity, when measured with 4-methylumbelliferyl-alpha- d-glucoside as a substrate, both in a complex bacterial community, in activated sludge and in pure cultures of bacterial isolates. we found that 140 microm cast inhibited alpha-glucosidase activity by 30% in a pure culture of pseudomonas s ... | 2002 | 12073134 |
| evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing. | several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ... | 2002 | 12149325 |
| genetic and biochemical characterization of cgb-1, an ambler class b carbapenem-hydrolyzing beta-lactamase from chryseobacterium gleum. | chryseobacterium gleum (previously included in the flavobacterium iib species) is a gram-negative aerobe that is a source of nosocomial infections. an ambler class b beta-lactamase gene was cloned and expressed in escherichia coli from reference strain c. gleum cip 103039 that had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. the purified beta-lactamase, cgb-1, with a pi value of 8.6 and a determined relative molecular mass of ca. 26 kda, hydrolyzed penicillins; nar ... | 2002 | 12183230 |
| ebr-1, a novel ambler subclass b1 beta-lactamase from empedobacter brevis. | empedobacter brevis (formerly designated flavobacterium breve) is a gram-negative aerobe involved in nosocomial infections. the ambler class b beta-lactamase gene bla(ebr-1) was cloned and expressed in escherichia coli from e. brevis clinical strain ass-1, which had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. purified beta-lactamase ebr-1 hydrolyzed penicillins, cephalosporins, and carbapenems efficiently but not aztreonam. kinetic parameters of ebr-1 were similar ... | 2002 | 12234848 |
| bacterial pathogens isolated from cultured bullfrogs (rana castesbeiana). | a commercial bullfrog (rana castesbeiana) operation in south georgia had multiple epizootics of systemic bacterial infections over a 3-year period, 1998-2000. a number of potential pathogens (aeromonas hydrophila, chryseobacterium (flavobacterium) meningosepticum, chryseobacterium (flavobacterium) indolgenes, edwardsiella tarda, citrobacterfreundii, pseudomonas spp., and (streptococcus iniae) were isolated from various tissues. clinically, frogs demonstrated acute onset of torticolis, stupor, an ... | 2002 | 12296400 |
| chromosome-encoded beta-lactamases tus-1 and mus-1 from myroides odoratus and myroides odoratimimus (formerly flavobacterium odoratum), new members of the lineage of molecular subclass b1 metalloenzymes. | myroides odoratus and myroides odoratimimus (formerly designated in a single species as flavobacterium odoratum) are gram-negative aerobes and sources of nosocomial infections in humans. they have variable susceptibility to beta-lactams and a decreased susceptibility to carbapenems. using genomic dnas of m. odoratus cip 103105 and m. odoratimimus cip 103073 reference strains, shotgun cloning of beta-lactamase genes was performed, followed by protein expression in escherichia coli. the deduced am ... | 2002 | 12384365 |
| molecular and biochemical characterization of a carbapenem-hydrolysing beta-lactamase from flavobacterium johnsoniae. | flavobacterium johnsoniae cip100931 is resistant to most beta-lactam antibiotics and has a decreased susceptibility to carbapenems. a beta-lactamase gene was cloned and expressed in escherichia coli dh10b. the purified beta-lactamase, john-1, with a pi value of 9.0 and with a determined relative molecular mass of approximately 27 kda was found to be a monomeric zinc-dependent enzyme that hydrolyses penicillins, narrow- and expanded-spectrum cephalosporins, carbapenems, but not monobactams. seque ... | 2003 | 12562690 |
| analysis of an outbreak due to chryseobacterium meningosepticum in a neonatal intensive care unit. | the aim of this study was to describe the epidemiological and clinical features of an outbreak due to chryseobacterium meningosepticum. during a 11-day period, the outbreak was observed among four newborns in a neonatal intensive care unit (nicu) in a teaching hospital. all patients yielded c. meningosepticum in their blood cultures, in addition one was colonised in the throat. antimicrobial susceptibility assay showed complete resistance to penicillins, cephalosporins, aminoglycosides, imipenem ... | 2003 | 12578312 |
| use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil. | seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from antarctica. the column was maintained at 10 degrees c and continuously fed with a casein-containing medium to enrich protease producers. twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological a ... | 2003 | 12620829 |
| chryseobacterium defluvii sp. nov., isolated from wastewater. | a gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterium (strain b2t) isolated from wastewater of a sequence batch reactor showing enhanced phosphorus removal was investigated to determine its taxonomic status. complete 16s rrna gene sequence analysis indicated that the organism should be placed in the genus chryseobacterium. the strain contained a polyamine pattern with sym-homospermidine as the major compound, menaquinone mk-6 as the predominant menaquinone and ai-c15:0, i-c1 ... | 2003 | 12656158 |
| characterization of a new keratinolytic bacterium that completely degrades native feather keratin. | a novel feather-degrading microorganism was isolated from poultry waste, producing a high keratinolytic activity when cultured on broth containing native feather. complete feather degradation was achieved during cultivation. the bacterium presents potential use for biotechnological processes involving keratin hydrolysis. chryseobacterium sp. strain kr6 was identified based on morphological and biochemical tests and 16s rrna sequencing. the bacterium presented optimum growth at ph 8.0 and 30 degr ... | 2003 | 12677362 |
| the 1.5-a structure of chryseobacterium meningosepticum zinc beta-lactamase in complex with the inhibitor, d-captopril. | the crystal structure of the class-b beta-lactamase, blab, from the pathogenic bacterium, chryseobacterium meningosepticum, in complex with the inhibitor, d-captopril, has been solved at 1.5-a resolution. the enzyme has the typical alphabeta/betaalpha metallo-beta-lactamase fold and the characteristic two metal binding sites of members of the subclass b1, in which two zn2+ ions were identified. d-captopril, a diastereoisomer of the commercial drug, captopril, acts as an inhibitor by displacing t ... | 2003 | 12684522 |
| the molecular class c acid phosphatase of chryseobacterium meningosepticum (olpa) is a broad-spectrum nucleotidase with preferential activity on 5'-nucleotides. | the olpa gene of chryseobacterium meningosepticum, encoding a molecular class c phosphatase, was cloned and expressed in escherichia coli. the gene encodes a 29-kda polypeptide containing an amino-terminal signal peptide typical of bacterial membrane lipoproteins. expression in e. coli results in a functional product that mostly partitions in the outer membrane. a secreted soluble olpa derivative (solpa) lacking the n-terminal cysteine residue for lipid anchoring was produced in e. coli and puri ... | 2003 | 12758163 |
| chryseobacterium joostei sp. nov., isolated from the dairy environment. | among a large collection of south african dairy isolates, a novel chryseobacterium taxon (dna group 3) was previously delineated by a polyphasic taxonomic study (hugo et al., syst appl microbiol 22, 586-595, 1999). in the present paper, this taxon is further characterized using 16s rdna sequencing, fatty acid methyl ester analysis and a comparative phenotypic analysis, resulting in the proposal of a novel species, chryseobacterium joostei sp. nov. (type strain ix 5a(t) = lmg 18212(t) = ccug 4666 ... | 2003 | 12807199 |
| occurrence and antimicrobial resistance of gram-negative bacteria isolated in haemodialysis water and dialysate of renal units: results of a greek multicentre study. | to evaluate the occurrence, identity and antimicrobial resistance of gram-negative bacteria isolated from municipal water supplies, treated water, and dialysate of all 85 greek haemodialysis centres. | 2003 | 12807469 |
| peritonitis caused by chryseobacterium meningosepticum in a patient undergoing continuous ambulatory peritoneal dialysis. | chryseobacterium meningosepticum is rarely encountered as a pathogen causing peritonitis in adults. a 54-year-old woman who underwent continuous ambulatory peritoneal dialysis for 8 years developed peritonitis due to c. meningosepticum. although she received intravenous antibiotics with good in vitro activity against the organism, the fever and signs of peritonitis persisted. the tenckhoff catheter was finally removed on the 25th day of hospitalization and the fever subsided. four isolates of c. ... | 2003 | 12833193 |
| establishment of a multiplex pcr system to diagnose tuberculosis and other bacterial infections. | in order to rapidly diagnose and differentiate tuberculosis from other bacterial infections, a 16s rrna gene (16s rdna)-directed multiplex pcr system was developed. in this system, a pair of universal primers and a tubercle bacillus (tb)-specific primer were designed based on highly conserved regions and tb species-specific variable region of bacterial 16s rdna. a 360 bp fragment was detected in all bacteria tested, and a 210 bp fragment was found only in tb. 19 species of known bacteria includi ... | 2000 | 12840925 |
| chryseobacterium meningosepticum keratitis. | to describe a case of a corneal ulcer caused by chryseobacterium meningosepticum in an eye without significant comorbidities. | 2003 | 12888063 |
| a chryseobacterium meningosepticum outbreak in a neonatal ward. | to report epidemiologic, bacteriologic, and clinical features of a chryseobacterium meningosepticum outbreak. | 2003 | 12940584 |
| new bioactive diterpene polyesters from euphorbia decipiens. | a reinvestigation with a modified extraction procedure of euphorbia decipiens resulted in the isolation and structure elucidation of three new myrsinane-type diterpene esters (1-3). the structures of compounds 1-3 were elucidated by spectroscopic data interpretation. compound 1 showed inhibitory activity against prolyl endopeptidase (pep), whereas compound 2 exhibited dna-damaging activity in a mutant yeast bioassay. | 2003 | 14510601 |
| comparative challenge model of flavobacterium columnare using abraded and unabraded channel catfish, ictalurus punctatus (rafinesque). | the early entry of the fish pathogen flavobacterium columnare and enhancement by abrasion was studied in channel catfish, ictalurus punctatus (rafinesque), using the polymerase chain reaction and a species-specific primer set for a bacterial 16s rrna gene product. evaluations were conducted following an abrasion bath immersion challenge with f. columnare. abrasion, a practice which has historically been used prior to bacterial challenge, had significant effects on the early entry of the pathogen ... | 2003 | 14513970 |
| characterization of a soil-derived bacterial consortium degrading 4-chloroaniline. | a bacterial consortium comprising four different species was isolated from an indonesian agricultural soil using a mixture of aniline and 4-chloroaniline (4ca) as principal carbon sources. the four species were identified as chryseobacterium indologenes sb1, comamonas testosteroni sb2, pseudomonas corrugata sb4 and stenotrophomonas maltophilia sb5. growth studies on aniline and 4ca as single and mixed substrates demonstrated that the bacteria preferred to grow on and utilize aniline rather than ... | 2003 | 14600240 |
| horizontal transfer of segments of the 16s rrna genes between species of the streptococcus anginosus group. | the nature in variation of the 16s rrna gene of members of the streptococcus anginosus group was investigated by hybridization and dna sequencing. a collection of 708 strains was analyzed by reverse line blot hybridization. this revealed the presence of distinct reaction patterns representing 11 different hybridization groups. the 16s rrna genes of two strains of each hybridization group were sequenced to near-completion, and the sequence data confirmed the reverse line blot hybridization result ... | 2003 | 14645285 |
| legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature. | following the initial discovery of two legume-nodulating burkholderia strains (l. moulin, a. munive, b. dreyfus, and c. boivin-masson, nature 411:948-950, 2001), we identified as nitrogen-fixing legume symbionts at least 50 different strains of burkholderia caribensis and ralstonia taiwanensis, all belonging to the beta-subclass of proteobacteria, thus extending the phylogenetic diversity of the rhizobia. r. taiwanensis was found to represent 93% of the mimosa isolates in taiwan, indicating that ... | 2003 | 14645288 |
| chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station mir. | classification of strain w3-b1, which was isolated from condensation water in the russian space laboratory mir, was investigated by a polyphasic taxonomic approach. cells of strain w3-b1 were nonmotile, asporogenous, gram-negative slender rods with rounded ends. 16s rrna gene sequence analysis indicated that organism should be placed in the genus chryseobacterium. this organism contains menaquinone mk-6 as the predominent isoprenoid quinone and 3-oh iso 17:0 (40%), iso 15:0 (33%) as the major fa ... | 2003 | 14666980 |
| antimicrobial susceptibility and epidemiology of a worldwide collection of chryseobacterium spp: report from the sentry antimicrobial surveillance program (1997-2001). | limited data are available on chryseobacterium spp. leading to an evaluation of the patient demographics and susceptibility patterns for chryseobacterium spp. collected in the first 5 years of the sentry antimicrobial surveillance program (1997 to 2001). fifty isolates (24 chryseobacterium meningosepticum, 20 chryseobacterium indologenes, two chryseobacterium gleum, and 4 chryseobacterium spp. isolates) were collected. the highest chryseobacterium prevalence was detected among the elderly. the m ... | 2004 | 14715802 |
| chryseobacterium indologenes bacteremia in a bone marrow transplant recipient with chronic graft-versus-host disease. | we describe a case of chryseobacterium indologenes bacteremia in a leukemia patient with chronic graft-versus-host disease (gvhd) 6 months after allogeneic bone marrow transplantation. blood cultures from a vein and via hickman catheter grew c. indologenes. the patient was successfully treated with piperacillin/tazobactam and the infection did not recur. our case indicates that c. indologenes infection can occur in patients with gvhd after allogeneic bmt and might be treated with a single agent, ... | 2003 | 14723368 |
| septic arthritis caused by chryseobacterium meningosepticum in an elbow joint prosthesis. | chryseobacterium meningosepticum is a gram-negative bacillus historically associated with meningitis and sepsis in premature neonates. clinicians should suspect this organism when gram-negative bacilli are seen on gram-stain and culture, particularly in immunocompromised patients, and in cases of disrupted host tissue integrity. we report the first case of septic arthritis due to this organism. | 2004 | 14746428 |
| [infectious complication caused by bacteria chryseomonas luteola in a patient with a severe combined injury of the head, abdomen and extremities]. | | 2003 | 14997825 |
| characteristics of nosocomial bacterial meningitis in children. | nosocomial meningitis is uncommon in children. we reviewed the medical records of all children who developed bacterial meningitis at least 72 hours after admission to mackay memorial hospital for the period july 1992 through june 2000. clinical manifestations, predisposing factors, pathogens, and outcomes were analyzed. twenty-two cases of nosocomial meningitis were identified, comprising 9.2% (22/239) of all pediatric cases of bacterial meningitis during the study period. the male-to-female rat ... | 2004 | 15060685 |
| amoebae-resisting bacteria isolated from human nasal swabs by amoebal coculture. | amoebae feed on bacteria, and few bacteria can resist their microbicidal ability. amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (arb), which may be human pathogens. to isolate new arb, we performed amoebal coculture from 444 nasal samples. we recovered 7 (1.6%) arb from 444 nasal swabs, including 4 new species provisionally named candidatus roseomonas massiliae, c. rhizobium massiliae, c. chryseobacterium massiliae, and c. amoebinatus massiliae. t ... | 2004 | 15109415 |
| systematic 16s rrna gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans. | clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16s rrna gene sequence analysis. each isolate yielded a > or =1,400-bp sequence containing <5 ambiguities which was compared with the genbank 16s rrna gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (< or =10 cases reported in the literature) human pathogens. eleven new species, "actinobaculum massiliae," ... | 2004 | 15131188 |
| proposal to transfer 'aegyptianella ranarum', an intracellular bacterium of frog red blood cells, to the family flavobacteriaceae as 'candidatus hemobacterium ranarum' comb. nov. | 'aegyptianella ranarum' (order rickettsiales), an ultrastructurally defined small, gram-negative rod, is known to replicate in the red blood cells of frogs. heretofore, this bacterium has not been characterized genetically. we cloned and sequenced the 16s rrna (1310 bp) and gyrb (718 bp) genes of 'a. ranarum' from a canadian frog blood specimen. in situ hybridization (with an 'a. ranarum' 16s rrna gene polymerase chain reaction product as probe) and electron microscopy confirmed that 'a. ranarum ... | 2004 | 15142244 |
| potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. | heterotrophic plate counts (hpcs) are commonly used to assess the general microbiological quality of drinking water. drinking water quality specifications worldwide recommend hpc limits from 100 to 500 cfu ml(-1). a number of recent studies revealed evidence that these bacteria may not be as harmless as generally accepted. it appears that immuno-compromised individuals are particularly at risk. this would include the very young and very old patients with diseases such as aids and patients on the ... | 2004 | 15145586 |
| culture-independent analysis of midgut microbiota in the arbovirus vector culicoides sonorensis (diptera: ceratopogonidae). | differences in midgut microbial communities inhabiting culicoides spp., insect vectors of virus pathogens, may affect the variation observed in the ability of these biting midges to propagate arthropod-borne viruses. as a first step toward addressing this hypothesis, midgut bacterial communities were compared between culicoides species expected to be efficient and inefficient vectors of virus pathogens. we used 16s rdna sequence and restriction fragment information to provisionally identify 36 b ... | 2004 | 15185934 |
| clinical and microbiological analysis of bloodstream infections caused by chryseobacterium meningosepticum in nonneonatal patients. | chryseobacterium meningosepticum bloodstream infections in 11 nonneonatal patients were reported. more than half of the infections were community acquired. pcr assays indicated that the organisms produced extended-spectrum beta-lactamases as well as metallo-beta-lactamases. genotyping showed diverse fingerprints among the isolates. six patients survived without appropriate antibiotic treatment. host factors are the major determinant of the outcomes of c. meningosepticum infections. | 2004 | 15243115 |
| comparative biochemical analysis of three bacterial prolyl endopeptidases: implications for coeliac sprue. | prolyl endopeptidases have potential for treating coeliac sprue, a disease of the intestine caused by proteolytically resistant peptides from proline-rich prolamins of wheat, barley and rye. we compared the properties of three similar bacterial prolyl endopeptidases, including the known enzymes from flavobacterium meningosepticum (fm) and sphingomonas capsulate (sc) and a novel enzyme from myxococcus xanthus (mx). these enzymes were interrogated with reference chromogenic substrates, as well as ... | 2004 | 15245330 |
| identification of carbohydrate degrading bacteria in sub-tropical regions. | several bacteria from the islands of puerto rico, vieques and trinidad were isolated for their carbohydrate degrading activities. these terrestrial and marine bacterium were collected from pineapple agricultural lands, tropical rain forests, coastlands and mangrove swamps. organisms were screened for activity using chromogenic substrates (azcl megazyme international ltd., ireland). the media composition for the effective culturing of some of the marine organisms has also been standardized. gram- ... | 2003 | 15264573 |
| three cases of post-traumatic endophthalmitis caused by unusual bacteria. | three cases of post-traumatic endophthalmitis caused by unusual bacteria are presented. the pathogens identified were: (i) bacillus cereus and citrobacter freundii; (ii) pseudomonas fluorescens; and (iii) chryseobacterium meningosepticum and stenotrophomonas maltophilia. two of these pathogens have not previously been reported to cause endophthalmitis. the available literature regarding the individual cases is summarized and a brief discussion of post-traumatic endophthalmitis is presented, with ... | 2004 | 15281988 |
| chryseobacterium meningosepticum septicemia in a recipient of allogeneic cord blood transplantation. | | 2004 | 15307598 |
| chryseobacterium meningosepticum infections in a dialysis unit. | chryseobacterium species are gram-negative bacteria with an unusual antibiotic profile. chryseobacterium meningosepticum is the species most commonly encountered as a human pathogen. | 2004 | 15334801 |
| an adult case of chryseobacterium meningosepticum meningitis. | chryseobacterium meningosepticum is an uncommon pathogen causing adult bacterial meningitis. herein, we report the case history of one 21-year-old woman with this uncommon central nervous system infection. a diagnosis of adult c. meningosepticum meningitis can only be confirmed by a positive cerebrospinal fluid (csf) culture. the patient had insulin-dependent diabetes mellitus as the underlying condition associated with this infection. the clinical presentations were fever, headache, consciousne ... | 2004 | 15507780 |
| in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients. | owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ... | 2004 | 15523180 |
| effects of enzymatic deamidation by protein-glutaminase on structure and functional properties of alpha-zein. | the performance of novel protein-glutaminase (pg) purified from chryseobacterium proteolyticumon alpha-zein was investigated. highly insoluble alpha-zein was able to be deamidated to the extent of deamidation degree 62% by using 50 mm potassium phosphate (ph 8) containing 11.7% ethanol, at 40 degrees c for 137 h. analysis by sodium dodecyl sulfate polyacrylamide-gel electrophoresis showed that deamidated and non-deamidated zeins have different mobilities. results of circular dichroism spectra re ... | 2004 | 15537323 |
| kaistella koreensis gen. nov., sp. nov., a novel member of the chryseobacterium-bergeyella-riemerella branch. | gram-negative, non-spore-forming, rod-shaped, yellow-pigmented bacteria isolated from a freshwater stream in korea were investigated to determine their taxonomic position. complete 16s rrna gene sequence analysis indicated that the organisms should be placed in the chryseobacterium-bergeyella-riemerella branch in the family flavobacteriaceae. phylogenetically, the strains were most closely related to chryseobacterium balustinum atcc 33487(t) and chryseobacterium scophthalmum lmg 13028(t) (94.3 a ... | 2004 | 15545478 |