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life cycle of ixodes (ixodes) loricatus (acari: ixodidae) under laboratory conditions.the life cycle of ixodes (ixodes) loricatus neumann, reared in the laboratory, is described. engorged females collected from opossums trapped in the states of minas gerais and são paulo, brazil, which were used to start the laboratory colonies, were designated as bmg and csp, respectively. larval and nymphal ticks from both colonies fed separately on rattus norvergicus berkenhout or calomys callosus rengger, whereas didelphis marsupialis l and didelphis albiventris lund were used as hosts for bm ...200011004783
natural infection, transovarial transmission, and transstadial survival of rickettsia bellii in the tick ixodes loricatus (acari: ixodidae) from brazil.an ixodes loricatus engorged female, infected with rickettsia bellii, was collected from an opossum (didelphis aurita) in mogi das cruzes, são paulo state, brazil. two consecutive laboratory tick generations (f(1) and f(2)) reared from this single engorged female were evaluated for rickettsia infection by polymerase chain reaction (pcr) targeting specific rickettsia genes. immature ticks fed on naïve wistar rats (rattus norvegicus) and adult ticks fed on opossum (d. aurita), both free of ticks a ...200617114723
the ticks (acari: ixodida: argasidae, ixodidae) of paraguay.the ticks reported in paraguay, which are here reviewed, can be categorized as 'endemic or established' (argas persicus or a sibling species, ornithodoros hasei, o. rostratus, o. rudis, o. talaje/o. puertoricensis, amblyomma aureolatum, am. auricularium, am. brasiliense, am. cajennense, am. calcaratum, am. coelebs, am. dissimile, am. dubitatum, am. incisum, am. longirostre, am. nodosum, am. ovale, am. pacae, am. parvum, am. pseudoconcolor, am. rotundatum, am. scutatum, am. tigrinum, am. triste, ...200717362600
rickettsia infection in five areas of the state of são paulo, brazil.this study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of são paulo. eight flea species (adoratopsylla antiquorum antiquorum, ctenocephalides felis felis, polygenis atopus, polygenis rimatus, polygenis roberti roberti, polygenis tripus, rhopalopsyllus lugubris, and rhopalopsyllus lutzi lutzi), and five tick species (amblyomma aureolatum, amblyomma cajennense, amblyomma dubitatum, ixodes loricatus, and rhipicephalus sanguineus) were ...200718094887
[occurrence of ixodes loricatus neumann, 1899 (acari: ixodidae) parasitizing didelphis albiventris (lund, 1841) (didelphimorphia: didelphidae) in campo grande, ms].this is first report of occurence of ixodes in the state of mato grosso do sul and first description of ixodes loricatus neumann, 1899, parasitizing didelphis albiventris in campo grande, ms, brazil.200819245763
rickettsia bellii infecting amblyomma sabanerae ticks in el salvador.four amblyomma sabanerae ticks collected from a turtle (kinosternon sp.) in san miguel, el salvador, were found by molecular analysis to be infected by rickettsia bellii. we provide the first report of rickettsia bellii in central america, and the first report of a rickettsia species in el salvador.201223265378
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
risk factors for bartonella species infection in blood donors from southeast brazil.bacteria from the genus bartonella are emerging blood-borne bacteria, capable of causing long-lasting infection in marine and terrestrial mammals, including humans. bartonella are generally well adapted to their main host, causing persistent infection without clinical manifestation. however, these organisms may cause severe disease in natural or accidental hosts. in humans, bartonella species have been detected from sick patients presented with diverse disease manifestations, including cat scrat ...201626999057
host blood meal-dependent growth ensures transovarial transmission and transstadial passage of rickettsia sp. phylotype g021 in the western black-legged tick (ixodes pacificus).in this study, we explored the growth dynamics of rickettsia sp. phylotype g021 during transovarial transmission and transstadial passage by ixodes pacificus using real-time quantitative pcr. four parental engorged i. pacificus females were allowed to complete their developmental stages until the f2-generation eggs yielded unfed larvae. all eggs, larvae, nymphs, and adults tested through 2 generations were found to be infected with phylotype g021. hence, we conclude that the efficiency of transo ...201323876278
detection of rickettsia bellii and rickettsia amblyommii in amblyomma longirostre (acari: ixodidae) from bahia state, northeast brazil.studies investigating rickettsial infections in ticks parasitizing wild animals in the northeast region of brazil have been confined to the detection of rickettsia amblyommii in immature stages of amblyomma longirostre collected from birds in the state of bahia, and in immatures and females of amblyomma auricularium collected from the striped hog-nosed skunk (conepatus semistriatus) and armadillos (euphractus sexcinctus) in the state of pernambuco. the current study extends the distribution of r ...201526413074
phylogeography and demographic history of amblyomma variegatum (fabricius) (acari: ixodidae), the tropical bont tick.the genetic diversity of amblyomma variegatum (fabricius) from four caribbean islands and five african countries was compared by analyzing the sequences of three gene fragments, two mitochondrial (12srdna and d-loop-dl), and one nuclear (intergenic transcribed spacer 2 [its2]). genetic variability of the its2 dna fragment consisted of only uninformative single nucleotide mutations, and therefore this gene was excluded from further analyses. mitochondrial gene divergences among african population ...201222448720
survey of ticks (acari: ixodidae) and their rickettsia in an atlantic rain forest reserve in the state of são paulo, brazil.the current study investigated the occurrence of ticks and their rickettsiae in the serra do mar state park, which encompasses one of the largest atlantic rain forest reserves of brazil. from july 2008 to june 2009, a total of 2439 ticks (2,196 free living and 243 collected on hosts) was collected, encompassing the following 13 species: amblyomma aureolatum (pallas), amblyomma brasiliense aragao, amblyomma dubitatum neumann, amblyomma fuscum neumann, amblyomma incisum neumann, amblyomma longiros ...201020939390
isolation of rickettsia rhipicephali and rickettsia bellii from haemaphysalis juxtakochi ticks in the state of são paulo, brazil.in the present study, attempts to isolate rickettsia in cell culture were performed individually in seven specimens of haemaphysalis juxtakochi ticks collected in the state of são paulo (southeastern brazil). rickettsia was successfully isolated by the shell vial technique and established in vero cell culture from six ticks (six isolates). dna extracted from infected cells of these isolates was tested by pcr and dna sequencing, using genus-specific rickettsia primers targeting the genes glta, ht ...200717142361
the parasitism of immature stages of ixodes loricatus (acari: ixodidae) on wild rodents in argentina.ixodes loricatus has long been considered with strict-total specificity to new world marsupials. however, frequent findings of its immature stages on rodents suggest that these vertebrates play an important role in the tick's life cycle. aspects dealing with the ecology of sigmodontinae rodents infestation by i. loricatus are unknown. to contribute to the knowledge of the ecology of this tick species, environmental factors, as well as host species, sex and age, were evaluated to find association ...200516082931
a review of the ticks (acari, ixodida) of brazil, their hosts and geographic distribution - 1. the state of rio grande do sul, southern brazil.a review of the ticks (acari, ixodida) of the state of rio grande do sul, southern brazil, was completed as a step towards a definitive list (currently indicated as 12) of such species, their hosts and distribution. the ticks: argas miniatus (poultry), ixodes loricatus (opossums), amblyomma aureolatum (dogs), a. calcaratum (anteaters), a. cooperi (capybaras), a. nodosum (anteaters), a. tigrinum (dogs) (neotropical) and rhipicephalus sanguineus (dogs) (introduced, cosmopolitan, afrotropical) were ...200010904399
sigmodontinae rodents as hosts for larvae and nymphs of ixodes loricatus neumann, 1899 (acari: ixodidae).larvae and nymphs of ixodes loricatus neumann, 1899 ticks (confirmed by morphological characters and by comparison of 16s mitochondrial rdna sequences) were collected from sigmodontinae wagner, rodents in central and northern argentina and uruguay. a total of 100 larvae and 38 nymphs of i. loricatus were collected on the genera akodon meyen (n = 36 individuals), calomys waterhouse (n = 2), oligoryzomys bang in = 12), oxymycterus waterhouse (n = 9), and scapteromys waterhouse (n = 13). 72 larvae ...200415638143
ticks (acari: ixodidae) associated with small terrestrial mammals in the state of minas gerais, southeastern brazil.from june 2005 to november 2010, 43 small mammals encompassing 6 species of didelphimorphia, 8 species of rodentia, and 1 species of lagomorpha were found parasitized by ticks in the state of minas gerais, southeastern brazil. nine tick species, in total 186 specimens, were identified as follows: amblyomma cajennense (larvae and nymphs) on opossums and rodents; amblyomma ovale (nymphs) on rodents; amblyomma parvum (nymphs) on rodents; amblyomma coelebs (nymphs) on opossums; amblyomma dubitatum ( ...201222585005
ecology of the interaction between ixodes loricatus (acari: ixodidae) and akodon azarae (rodentia: criceridae).the present study explores associations of different factors (i.e. host parameters, presence of other ectoparasites and [mainly biotic] environmental factors) with burdens of ixodes loricatus immature stages in one of its main hosts in argentina, the rodent akodon azarae. for 2 years, rodents were trapped and sampled monthly at 16 points located in four different sites in the parana river delta region. data were analysed with generalized linear mixed models with a negative binomial response (cou ...201526122994
epidemiology of brazilian spotted fever in the atlantic forest, state of são paulo, brazil.the tick-borne bacterium rickettsia rickettsii is the aetiological agent of brazilian spotted fever (bsf). the present study evaluated tick infestations on wild and domestic animals, and the rickettsial infection in these animals and their ticks in 7 forest areas adjacent to human communities in the são paulo metropolitan area (spma). the results were compared to ecological traits of each sampled area. two main tick species, amblyomma aureolatum and rhipicephalus sanguineus, were collected from ...201222716923
factors affecting patterns of amblyomma triste (acari: ixodidae) parasitism in a rodent host.here we offer a multivariable analysis that explores associations of different factors (i.e., environmental, host parameters, presence of other ectoparasites) with the interaction of amblyomma triste immature stages and one of its main hosts in argentina, the rodent akodon azarae. monthly and for two years, we captured and sampled rodents at 16 points located at 4 different sites in the parana river delta region. the analyses were conducted with generalized linear mixed models with a negative bi ...201526104965
serologic evidence of the exposure of small mammals to spotted-fever rickettsia and rickettsia bellii in minas gerais, brazil.sources of pathogenic rickettsia in wildlife are largely unknown in brazil. in this work, potential tick vectors and seroreactivity of small mammals against four spotted-fever group rickettsia (r. rickettsii, r. parkeri, r. amblyommii and r. rhipicephali) and rickettsia bellii from peri-urban areas of uberlândia, a major town in brazil, are described for the first time.201627031460
microorganisms in ticks (acari: ixodidae) collected on marsupials and rodents from santa catarina, paraná and mato grosso do sul states, brazil.information about tick fauna and monitoring of pathogen prevalences in ticks (acari: ixodidae) in various habitat types can enhance knowledge about the epidemiology of tick-borne pathogens in brazil. this work shows the results of a study of tick parasitism of wild rodents and marsupials collected in seven localities in the southern part of brazil, within atlantic forest and cerrado biomes. a total of 61 ticks were collected from small mammals, and after identification to the species level, the ...201727769655
ectoparasite occurrence associated with males and females of wild rodents oligoryzomys flavescens (waterhouse) and akodon azarae (fischer) (rodentia: cricetidae: sigmodontinae) in the punta lara wetlands, argentina.the objective of this research was to study infestation parameters and indexes of ectoparasites associated with each sex of the wild rodents oligoryzomys flavescens (waterhouse) andakodon azarae (fischer) in the punta lara wetlands, argentina. a trend towards higher mean abundance (ma) and ectoparasite specific richness was observed in males of o. flavescens whereas those values were similar for both a. azarae sexes. the prevalence of the following ectoparasites was significantly higher on males ...201021120393
seasonality of immature stages of ixodes loricatus (acari: ixodidae) in the paraná delta, argentina.in this study we analyzed seasonal trends of immature stages of ixodes loricatus parasitizing akodon azarae (sigmodontinae) in a locality of the paraná delta, buenos aires, argentina. the data encompassed two consecutive years and the analysis included the control of potential confounders using generalized linear mixed models (glmm) with a negative binomial response. larvae had within-year differences in abundance, but the peak months differed drastically between both sampling years, indicating ...201425113986
ecology of a tick-borne spotted fever in southern brazil.rio grande do sul is the southernmost state of brazil, bordering uruguay. clinical cases of spotted fever group (sfg) rickettsiosis were recently reported in rio grande do sul. none of these cases was lethal, and all were confirmed by seroconversion to r. rickettsii antigens. because serological cross-reactions are well known to occur between different sfg agents, the sfg agent responsible for the clinical cases remains unknown in rio grande do sul, where no rickettsial agent is known to infect ...201627392739
taxonomic status of ixodes didelphidis (acari: ixodidae).ixodes didelphilis fonseca & aragão was described in brazil in 1952 as a new tick species that differed from ixodes loricatus neumann by the spiracular plate pattern. we have reared four tick colonies from different geographic areas in the laboratory that were started from single engorged females originally identified as i. didelphidis (bmg, colony ) and i. iocicatus (csp, psp, and trj colonies). we analyzed the spiracular plate morphology of f1 adult ticks from each tick colony, compared their ...200211931247
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