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[survey on tick vectors of lyme disease spirochetes in china].since 1987 we have been studying on lyme disease in china. there were 71 isolates of borrelia burgdorferi recovered from six species of ticks including ixodes persulcatus, ixodes granulatus, ixodes rangtangensis, haemaphysalis concinna. haemaphysalis bispinosa and haemaphysalis longicornis, and 55 isolates from ixodes persulcatus collected from heilongjiang, jilin, liaoning, nei-monggol, hebei and xinjiang region (province). ixodes persulcarus is the dominant species accounted for more than 80% ...19921300244
[investigation of rodents and ixodes for lyme disease and four strains of borrelia burgdorferi first isolated from ixodes granulatus supino, rattus confucianus and r. norvegicus in fujian province].with bsk ii medium, 4 strains of borrelia burgdorferi was isolated from ixodes granulatus supino, rattus confucianus and r, novregicus, which ixodes and ruttus all captured from forest areas in northern of fujian province where have found lyme cases. these isolated strains were identified by institute of epidemiology and microbiology, chinese academy of preventive medicine.19921301268
material for the study ofvariation in the malayan langat virus (tp-21 strain).the lack of genetic uniformity of the malayan langat virus, tp-21 strain isolated from ixodes granulatus ticks in malaya, was demonstrated.increased pathogenicity of the virus for white mice and rhesus monkeys after passage through chick-embryo fibroblast cultures at temperatures of 36 degrees c and 40 degrees c is due to the selection of the pathogenic virus particles which are always present in the initial brain-tissue suspension of the tp-21 langat strain.the frequency of the occurrence of pa ...19684973556
lyme disease in taiwan: first human patient with characteristic erythema chronicum migrans skin lesion.we report herein the first laboratory-diagnosed case of lyme disease in a human in taiwan. a 45-year-old taiwanese man living in taipei, in northern taiwan, had an expanding skin lesion (measuring 23 by 15 cm) on his abdomen for 2 to 3 weeks and recurrent attacks of pain and swelling of the knee joint. serologic tests indicated a significantly elevated titer of antibody to borrelia burgdorferi. after appropriate antibiotic treatment for 3 weeks, the skin lesion was cured and the joint swelling w ...19989508317
[investigation on primary vectors of borrelia burgdorferi in china].from 1987 to 1997, primary vectors of borrelia burgdorferi sensu lato (b.b.s.l.) were investigated in china. a total number of 17,000 ticks collected from 20 provinces, city and autonomous region were classified, including 2 families 8 genus 23 species. there were 108 isolates of b.b.s.l. discovered from 8 species of ticks, including ixodes persulcatus, i. granulatus, i. acutitarsus, haemaphysalis concinna, h. longicornis, h. bispinosis, h. cornigera taiwana and dermacentor silvarum. ixodes pers ...199810322682
identification of ehrlichia chaffeensis by nested pcr in ticks from southern china.a total of 717 ticks collected from southern china were examined by nested pcr for the presence of ehrlichia chaffeensis. sixteen (55. 2%) of 29 adult amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) haemaphysalis yeni ticks tested positive. four other species of ticks were negative. selected positive amplicons were confirmed by dna sequencing.200010878087
short report: thai tick typhus, rickettsia honei, and a unique rickettsia detected in ixodes granulatus (ixodidae: acari) from thailand.a unique rickettsia species (rickettsia thailandii sp. nov.) was identified in an ixodes granulatus by use of polymerase chain reaction heteroduplex mobility assay by use of segments of the citrate synthase gene. this tick was collected from rattus rattus from nakhon ratchasima province in 1970. another i. granulatus was infected with thai tick typhus strain tt-118, rickettsia honei sp. nov. stenos, roux, walker & raoult; this tick was removed from a r. rattus collected 4 years later from the sa ...200111716110
emerging rickettsioses of the thai-myanmar border.to investigate the presence of rickettsioses in rural residents of the central thai-myanmar border, we tested the blood of 46 patients with fever. four patients had murine typhus, three patients had scrub typhus, and eight patients had spotted fever group rickettsioses, including the first case of rickettsia felis infection reported in asia.200312737744
evaluation of groel gene analysis for identification of borrelia burgdorferi sensu lato.the nucleotide sequences of the groel genes, the flagellin genes, and the 16s rrna genes from 22 reference strains of borrelia were compared. groel sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of borrelia afzelii and borrelia garinii isolates.200415004091
tick-borne rickettsioses around the world: emerging diseases challenging old concepts.during most of the 20th century, the epidemiology of tick-borne rickettsioses could be summarized as the occurrence of a single pathogenic rickettsia on each continent. an element of this paradigm suggested that the many other characterized and noncharacterized rickettsiae isolated from ticks were not pathogenic to humans. in this context, it was considered that relatively few tick-borne rickettsiae caused human disease. this concept was modified extensively from 1984 through 2005 by the identif ...200516223955
a survey of ectoparasites in gunung stong forest reserve, kelantan, malaysia.a survey of ticks and other ectoparasites was carried out during a national biodiversity scientific expedition at gunung stong forest reserve, kelantan, malaysia from 23-29 may 2003. a total of 272 animals comprised of 12 species of birds, 21 species of bats, 7 species of rodents and 2 species of insects were examined for ticks and other ectoparasites. from these animals, 5 species in 4 genera of ticks; 7 species in 2 families of mesostigmatid mites and 5 species of chiggers were collected. amon ...200516438136
detection of borrelia garinii, borrelia tanukii and borrelia sp. closely related to borrelia valaisiana in ixodes ticks removed from dogs and cats in japan.ticks removed from 1136 dogs and 134 cats all over japan were examined for borrelia infection by pcr and sequencing. the 5s-23s rdna intergenic spacer of borrelia was detected from two ixodes persulcatus ticks from two dogs and two unidentified ixodes spp. from another two dogs in hokkaido, and two ixodes granulatus ticks from two cats in okinawa. additional 2 i. granulatus from the same cats also showed positive. sequence analysis of the pcr products revealed that the one from hokkaido was simi ...200717049744
ticks (ixodidae) and other ectoparasites in ulu muda forest reserve, kedah, malaysia.a survey of ticks and other ectoparasites was carried out during a national biodiversity scientific expedition at ulu muda forest reserve, kedah, malaysia from 23-29 march 2003. a total of 161 animals comprising 20 species of birds, 16 species of bats, six species of non-volant small mammals and 12 species of reptiles were examined for ticks and other ectoparasites. from these animals, nine species in five genera of ticks, 10 species in two families of mesostigmatid mites and five species of chi ...200818564690
novel genospecies of borrelia burgdorferi sensu lato from rodents and ticks in southwestern china.by using multilocus sequence analysis, five borrelia valaisiana-related strains isolated from rodents and ticks in southwestern china were eventually classified as a new genospecies of b. burgdorferi sensu lato rather than b. valaisiana. the finding explained the differences in transmission cycle and phenotype between b. valaisiana strains from europe and b. valaisiana-related strains from eastern asia.200818614645
isolation and identification of a novel spotted fever group rickettsia, strain ig-1, from ixodes granulatus ticks collected on orchid island (lanyu), taiwan.a novel species of spotted fever group (sfg) rickettsia, rickettsia spp. ig-1, was identified and isolated from adult ixodes granulatus ticks collected from orchid island (lanyu), an islet located in southeastern taiwan. serum samples collected from the rodent host rattus losea and sfg-positive human serum reacted with ig-1 using an immunofluorescence assay (ifa). pairwise nucleotide sequence analysis of 16s rrna (rrs), glta, ompa, ompb, and sca4 shows that ig-1 belongs to sfg rickettsiae and ha ...200818689633
rickettsia sp. in ixodes granulatus ticks, japan. 200819046539
human case of rickettsia felis infection, taiwan. 200819046543
first detection and molecular identification of borrelia burgdorferi-like spirochetes in ixodes granulatus ticks collected on kinmen island of taiwan.borrelia burgdorferi-like spirochetes were detected for the first time in ixodes granulatus ticks collected in taiwan. the genetic identities of these detected spirochetes were determined by analyzing the gene sequences amplified by a genospecies-specific polymerase chain reaction assay based on the outer surface protein a (ospa) gene of b. burgdorferi sensu lato. phylogenetic analysis reveals that the sequence similarity of these spirochetes, ranging from 90.5% to 98.7% is highly homogeneous wi ...200919270287
spotted fever group rickettsia sp. closely related to rickettsia japonica, thailand. 200919331747
ectoparasite fauna of rodents and shrews from four habitats in kuala lumpur and the states of selangor and negeri sembilan, malaysia and its public health significance.a total of 204 rodents comprising 14 host species from four different habitats were examined. nine rodent species were trapped from the forest and another five species were trapped from the coastal, rice field and urban habitats. rattus rattus diardii (67%) was the predominant rodent species examined. fifty six (47.3%) rodents and shrews were found to be infested with at least one of the 20 species of ectoparasite recovered. mites belonging to the family trombiculidae were the predominant ectopa ...200920237444
distribution of borrelia burgdorferi sensu lato in china.we genotyped 102 borrelia burgdorferi sensu lato strains isolated from ticks, animals, and patients in 11 provinces in china by pcr-restriction fragment length polymorphism (pcr-rflp) amplification of 5s (rrf)-23s (rrl) rrna gene spacer amplicons and multilocus sequence analysis (mlsa). the results showed that borrelia garinii was the main genotype in china (65/102) and that it was distributed mainly in northern china. borrelia afzelii was the second most frequently found species (22/102), and i ...201021106783
arthropod symbiotes of laonastes aenigmamus (rodentia: diatomyidae).abstract arthropod symbiotes of the laotian rock-rat, laonastes aenigmamus (rodentia: diatomyidae), from laos are examined. this host is a member of diatomyidae previously thought to have gone extinct >10 million yr ago. permanent symbiotes are represented by 2 species, a new species of sucking louse, polyplax sp., near rhizomydis (phthiraptera: polyplacidae), and a new species of fur mite, afrolistrophorus sp., near maculatus (acariformes: listrophoridae). the temporary parasites are represente ...201121506873
Rickettsia honei infection in human, Nepal, 2009.We report a case of Rickettsia honei infection in a human in Nepal. The patient had severe illness and many clinical features typical of Flinders Island spotted fever. Diagnosis was confirmed by indirect immunofluorescent assay with serum and molecular biological techniques. Flinders Island spotted fever may be an endemic rickettsiosis in Nepal.201122000356
distribution of tick-borne diseases in china.as an important contributor to vector-borne diseases in china, in recent years, tick-borne diseases have attracted much attention because of their increasing incidence and consequent significant harm to livestock and human health. the most commonly observed human tick-borne diseases in china include lyme borreliosis (known as lyme disease in china), tick-borne encephalitis (known as forest encephalitis in china), crimean-congo hemorrhagic fever (known as xinjiang hemorrhagic fever in china), q-f ...201323617899
autochthonous lyme borreliosis in humans and ticks in korea.this study aimed at finding epidemiological and clinical features of autochthonous lyme borreliosis in humans through epidemiological investigations and identifying its vectors and pathogens through analysis of ticks.201324159530
emerging tick-borne infections in mainland china: an increasing public health threat.since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ...201526453241
ixodes scapularis and ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis.ixodid ticks are important vectors of a wide variety of viral, bacterial and protozoan pathogens of medical and veterinary importance. although several studies have elucidated tick responses to bacteria, little is known about the tick response to viruses. to gain insight into the response of tick cells to flavivirus infection, the transcriptomes and proteomes of two ixodes spp cell lines infected with the flavivirus tick-borne encephalitis virus (tbev) were analysed.201526582129
induction and suppression of tick cell antiviral rnai responses by tick-borne flaviviruses.arboviruses are transmitted by distantly related arthropod vectors such as mosquitoes (class insecta) and ticks (class arachnida). rna interference (rnai) is the major antiviral mechanism in arthropods against arboviruses. unlike in mosquitoes, tick antiviral rnai is not understood, although this information is important to compare arbovirus/host interactions in different classes of arbovirus vectos. using an ixodes scapularis-derived cell line, key argonaute proteins involved in rnai and the re ...201425053841
detection of langat virus by taqman real-time one-step qrt-pcr method.langat virus (lgtv), one of the members of the tick-borne encephalitis virus (tbev) complex, was firstly isolated from ixodes granulatus ticks in malaysia. however, the prevalence of lgtv in ticks in the region remains unknown. surveillance for lgtv is therefore important and thus a tool for specific detection of lgtv is needed. in the present study, we developed a real-time quantitative reverse-transcription-polymerase chain reaction (qrt-pcr) for rapid detection of lgtv. our findings showed th ...201526360297
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
acarine ectoparasites of panti forest reserve in johore, malaysia.to identify the presence of acarine ectoparasites and determine whether there is any potential public health risk in panti forest reserve, johore, malaysia.023569714
rickettsia felis, an emerging flea-transmitted human pathogen.rickettsia felis was first recognised two decades ago and has now been described as endemic to all continents except antarctica. the rickettsiosis caused by r. felis is known as flea-borne spotted fever or cat-flea typhus. the large number of arthropod species found to harbour r. felis and that may act as potential vectors support the view that it is a pan-global microbe. the main arthropod reservoir and vector is the cat flea, ctenocephalides felis, yet more than 20 other species of fleas, tick ...201124149035
severe fever with thrombocytopenia syndrome virus in ticks collected from humans, south korea, 2013.we investigated the infection rate for severe fever with thrombocytopenia syndrome virus (sftsv) among ticks collected from humans during may-october 2013 in south korea. haemaphysalis longicornis ticks have been considered the sftsv vector. however, we detected the virus in h. longicornis, amblyomma testudinarium, and ixodes nipponensis ticks, indicating additional potential sftsv vectors.025061851
ectoparasites of murids in peninsular malaysia and their associated diseases.a considerable number of rat-borne ectoparasite studies have been conducted since the early 1930s in the malayan peninsula (now known as peninsular malaysia). the majority of studies were field surveys and collections of specimens across the region, and were conducted primarily to catalogue the ectoparasite host distribution and discover novel species. this has generated a signification amount of information, particularly on the diversity and host distribution; other aspects such as morphology, ...201525924677
a tick-borne segmented rna virus contains genome segments derived from unsegmented viral ancestors.although segmented and unsegmented rna viruses are commonplace, the evolutionary links between these two very different forms of genome organization are unclear. we report the discovery and characterization of a tick-borne virus--jingmen tick virus (jmtv)--that reveals an unexpected connection between segmented and unsegmented rna viruses. the jmtv genome comprises four segments, two of which are related to the nonstructural protein genes of the genus flavivirus (family flaviviridae), whereas th ...201424753611
molecular epidemiology of orientia tsutsugamushi in chiggers and ticks from domestic rodents in shandong, northern china.scrub typhus is endemic to a 13,000,000-km² area of the asia-pacific region, and causes an annual incidence of 1 million people. the mortality rate of scrub typhus ranges from 6.1% to 25% in southeast asia. natural infection of orientia tsutsugamushi has been identified in domestic rodents in shandong province. however, infestation of chiggers and ticks on the domestic rodents and prevalence and genotypes of o. tsutsugamushi in these acarina remain unclear.201324499627
biodiversity and macroparasitic distribution of the wild rat population of carey island, klang.a study to determine the diversity and distribution of ectoparasites and endoparasites infesting wild rat population of carey island was carried out from june to december 2010. a total of 81 rats were captured from various locations on carey island. four rat species were identified namely, rattus tiomanicus (45.7%), rattus rattus diardii (25.9%), rattus argentiventer (16%) and rattus norvegicus (12.3%). low diversity of ecto and endoparasites were observed infecting the rodent population with 8 ...201323959485
high prevalence of rickettsia spp. infections in small mammals in taiwan.surveillance for rickettsia spp. is urgently needed due to the recent emergence of many novel rickettsioses around the globe, but previous studies in taiwan have been limited to small areas and no investigation of infections in vertebrate hosts has ever been attempted. we surveyed rickettsial infections systematically in small-mammal hosts trapped between 2006 and 2010 throughout taiwan. fragments of ompb and glta genes in the liver, spleen, and kidney of mammals were targeted by nested polymera ...025629776
nested coevolutionary networks shape the ecological relationships of ticks, hosts, and the lyme disease bacteria of the borrelia burgdorferi (s.l.) complex.the bacteria of the borrelia burgdorferi (s.l.) (bbg) complex constitute a group of tick-transmitted pathogens that are linked to many vertebrate and tick species. the ecological relationships between the pathogens, the ticks and the vertebrate carriers have not been analysed. the aim of this study was to quantitatively analyse these interactions by creating a network based on a large dataset of associations. specifically, we examined the relative positions of partners in the network, the phylog ...201627662832
prevalence of borrelia burgdorferi sensu lato in ticks from eastern china.to explore the tick distribution and prevalence of borrelia in zhejiang province, we performed a survey in nine sites. a total of 447 adult ticks of 11 species were captured and the dominant tick species were haemaphysalis longicornis and ixodes sinensis and the abundance of tick species in different areas varied significantly. overall, 4.70% of the ticks were polymerase chain reaction (pcr) positive for borrelia. the average pcr positive rates were 5.19% for h. longicornis, 3.45% for amblyomma ...201525548382
molecular survey of hard ticks in endemic areas of tick-borne diseases in china.over the past several years, there was a substantial increase in the number of cases of known and novel tick-borne infections in humans in china. to better understand the ticks associated with these infections, we collected hard ticks from animals or around livestock shelters in 29 localities in 5 provinces (beijing, henan, hubei, inner mongolia, and zhejiang) where cases of tick-borne illness were reported. we collected 2950 hard ticks representing 7 species of 4 genera (dermacentor sinicus, ha ...201323538111
complete genome sequencing of borrelia valaisiana and borrelia afzelii isolated from ixodes persulcatus ticks in western siberia.lyme disease, caused by bacteria of the borrelia burgdorferi sensu lato complex, is the most frequent tick-borne infection in eurasia. here, we report the complete genome sequence of the borrelia valaisiana tom 4006 and borrelia afzelii tom 3107 strains isolated from ixodes persulcatus ticks in western siberia.201425540343
surveys of rodent-borne disease in thailand with a focus on scrub typhus assessment.the epidemiology of many rodent-borne diseases in south-east asia remains ill-defined. scrub typhus and lep-tospirosis are common and medically significant, while other zoonotic diseases, such as spotted fever group rickettsiae have been identified, but their overall medical significance is unknown. rodent surveillance was conducted from june 2002 to july 2004 in 18 provinces from thailand. traps were set up for one to three nights. blood and serum samples and animal tissue samples (liver, splee ...200821396076
[isolation and identification of borrelia burgdorferi sensu lato from ticks in six provinces in china.]objective: to understand the carrying status of borrelia burgdorferi in ticks from the mountain areas from six representative provinces, including jilin, shanxi, gansu, qinghai, guizhou and hunan in china. methods: flagging and trapping methods were used to collect ticks in forest area and culture medium was used to isolate the pathogen. nested-pcr was used to detect the germ-carrying rate of ticks. results: more than 2200 ticks from six representative provinces were collected and 1000 ticks wer ...201021223661
the presence of borrelia valaisiana-related genospecies in ticks and a rodent in taiwan.a field survey was conducted to investigate the presence of borrelia burgdorferi sensu lato (s.l.) in six counties of taiwan. spirochetes were successfully isolated from one rodent ear sample out of 485 rodent ears and 53 live, fed tick (ixodes granulatus) samples. the spirochetes were confirmed to be b. burgdorferi s.l. by real-time pcr. in addition, 23 of 113 tick samples were tested positive for borrelia dna according to real-time pcr. the borrelia isolate from the rodent and the 23 borrelia ...201021221950
molecular detection of borrelia valaisiana-related spirochetes from ixodes granulatus ticks in taiwan.borrelia valaisiana-related spirochetes were detected for the first time in ixodes granulatus ticks collected in taiwan. the genetic identities of these detected spirochetes were determined by analyzing the gene sequences amplified by a genospecies-specific polymerase chain reaction assay based on the outer surface protein a (ospa) gene of b. burgdorferi sensu lato. phylogenetic relationships were analyzed by comparing the sequences of ospa gene obtained from 35 strains of borrelia spirochetes r ...201020585840
presence of a novel ehrlichia sp. in ixodes granulatus found in okinawa, japan.ehrlichia-specific dna fragments of ehrlichia omp-1 and groel genes were found in two i. granulatus ticks which had been collected from wild small mammals in a subtropical zone in japan. the dna sequences of groel and 16srdna of the suspected ehrlichia were clustered into a group of e. chaffeensis, e. muris, and ehrlichia sp. hf565 found in i. ovatus, but were distinctly different. therefore the ehrlichia strain was designated as a novel ehrlichia sp. 360. the ehrlichia sp. 360 was detected in i ...200919291093
molecular analysis of ixodes granulatus, a possible vector tick for borrelia burgdorferi sensu lato in taiwan.the genetic identity of ixodes granulatus ticks was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 19 strains of ticks representing seven species of ixodes and two outgroup species (rhipicephalus sanguineus and haemaphysalis inermis). four major clades could be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granul ...200919184580
molecular detection and characterization of spotted fever group rickettsiae in taiwan.rickettsioses are emerging infectious diseases caused by rickettsiae in association with arthropods. we report the detection of spotted fever group rickettsiae (sfgr) in taiwan using molecular methods. phylogenetic analyses of the 17-kd protein and citrate synthase (glta) genes showed that sfgr twkm01 detected in rhipicephalus haemaphysaloides ticks was most similar to rickettsia rhipicephali. three twkm01 isolates were obtained from three individual r. haemaphysaloides ticks. small, intracellul ...200717984347
the tick ixodes granulatus infests rattus rattus populating a small island offshore of singapore.the ixodid tick ixodes granulosus supino 1897 was found infesting rattus rattus in semakau island, one of the small offshore islands fringing singapore to the south. none of the examined r. rattus from the other islands fringing singapore, or from singapore island were found infested. ixodes granulatus occurs, however, on singapore island on rattus annandalei, resident of undisturbed forested habitats. we speculate that invading black rats in semakau replaced autochthonous sylvatic rodent popula ...200616605073
terrestrial distribution of the lyme borreliosis agent borrelia burgdorferi sensu lato in east asia.lyme borreliosis is the most prevalent tick-borne zoonosis and an important emerging infection in europe, north america, and far eastern countries. the geographical distribution of borrelia spp. and the relationship between borrelia spp. and tick spp. in east asian countries have been studied. in northern asian countries, ixodes persulcatus carries eurasian-type borrelia garinii (20047 type), borrelia afzelii, and asian-type b. garinii (variant nt29), whereas borrelia burgdorferi sensu stricto h ...200415623946
ticks (acari: ixodidae) parasitizing wild carnivores in phu khieo wildlife sanctuary, thailand.ixodid ticks were collected and identified from 8 wild carnivore species in phu khieo wildlife sanctuary, northeastern thailand. six tick species belonging to 4 genera were recovered and identified from 132 individuals. these included amblyomma testudinarium (n = 36), haemaphysalis asiatica (n = 58), h. hystricis (n = 31), h. semermis (n = 3), rhipicephalus haemaphysaloides (n = 3), and ixodes granulatus (n = 1). leopard cats (prionailurus bengalensis) (n = 19) were infested with 4 tick species, ...200415270119
detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam.a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ...200312682151
survey of ixodid tick species on domestic cats in japan.various species of ixodid ticks, attached to domestic cats in japan, were identified in spring (april to june) and autumn (september to november). in the spring, a total of 282 ticks, including 61 larvae, 70 nymphs, 127 females and 24 males were collected from 126 cats. of these, 264 were identified up to the species level. in the spring, haemaphysalis longicornis was the most frequently (39.7%, 50/126) found tick species on feline hosts, followed by ixodes ovatus (35.0%, 44/126), ixodes nippone ...200312531297
borrelia sinica sp. nov., a lyme disease-related borrelia species isolated in china.a survey was performed for lyme disease borrelia in the southern part of china, in zhejiang, sichuan and anhui provinces, along the yangtze river valley, in may of 1997 and 1998. twenty isolates from ixodes granulatus, ixodes ovatus, apodemus agrarius and niviventer confucianus were obtained. these isolates were characterized by rflp of the 5s-23s rdna intergenic spacer, sequence analysis of the intergenic spacer, 16s rdna and flagellin gene, dna-dna hybridization analysis, sds-page and western ...200111594614
differentiation of borrelia burgdorferi sensu lato on the basis of rna polymerase gene (rpob) sequences.we determined the nucleotide sequences (329 bp) of the rpob dnas from 22 reference strains of borrelia. no insertions or deletions were observed. deduced amino acid sequences of amplified rpob dna comprised 109 amino acid residues (n(450) to m(558) [escherichia coli numbering]). all amino acid sequences were identical with the exception of those of borrelia lusitaniae potib2 (t(461)-->a) and b. bissettii dn127 (i(498)-->v). each species of b. burgdorferi sensu lato was differentiated as a distin ...200010878043
prevalence and molecular identification of borrelia spirochetes in ixodes granulatus ticks collected from rattus losea on kinmen island of taiwan.ixodes granulatus is widely distributed in various countries of southeast asia and taiwan. although this tick species is presumed to be the vector for the enzoonotic transmission of borrelia spirochetes in the taiwan area, the prevalence of infection and genetic diversity of borrelia spirochetes harbored by this tick species need to be further determined.201222882955
natural history of zoonotic babesia: role of wildlife reservoirs.babesiosis is an emerging zoonotic disease on all inhabited continents and various wildlife species are the principal reservoir hosts for zoonotic babesia species. the primary vectors of babesia are ixodid ticks, with the majority of zoonotic species being transmitted by species in the genus ixodes. species of babesia vary in their infectivity, virulence and pathogenicity for people. various factors (e.g., increased interactions between people and the environment, increased immunosuppression, ch ...201224533312
borrelia, rickettsia, and ehrlichia species in bat ticks, france, 2010.argas vespertilionis, an argasid tick associated with bats and bat habitats in europe, africa, and asia has been reported to bite humans; however, studies investigating the presence of vector-borne pathogens in these ticks are lacking. using molecular tools, we tested 5 a. vespertilionis ticks collected in 2010 from the floor of a bat-infested attic in southwestern france that had been converted into bedrooms. rickettsia sp. avbat, a new genotype of spotted fever group rickettsiae, was detected ...201223171714
characterization of borrelia burgdorferi strains isolated from korea by 16s rdna sequence analysis and pcr-rflp analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons.haenam strains of borrelia burgdorferi, which had been isolated from ixodes granulatus and apodemus agrarius in haenam, korea, were characterized by pcr-rflp analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons and by sequence analysis of the 16s rrna gene (rdna). the msel and dral restriction patterns of the 5s-23s intergenic spacer amplicons of haenam strains differed from those of other b. burgdorferi sensu lato strains. furthermore, in the phylogenetic tree based on the 16s rdna sequen ...200010758897
[studies on epidemiology and etiology of lyme disease in china].from 1987 to 1996, an investigation and study on lyme disease was conducted in sixty counties and districts of twenty-two provinces (autonomous region, municipality). seroepidemiological findings indicated that some residents from forest areas of twenty-two provinces had a high antibody titer for borrelia burgdorferi with positive rate 5.06% (1724/34104). endemic foci of lyme disease were discovered in seventeen provinces by etiological evidence. typical cases of lyme disease were diagnosed in e ...19979812472
ectoparasites of commensal rodents in sulawesi utara, indonesia, with notes on species of medical importance.ectoparasite records are presented for four species of commensal murid rodents (rattus rattus palelae miller & hollister, r. argentiventer (robinson & kloss), r. exulans (peale) and mus musculus castaneus waterhouse) in sulawesi utara, with particular reference to the potential for these arthropods to bite and transmit pathogens to humans. the flea, xenopsylla cheopis (rothschild), was most common on r.r. palelae and is capable of transmitting plague and other pathogens to humans although no cur ...19911768888
prevalence of antibodies against ehrlichia spp. and orientia tsutsugamushi in small mammals around harbors in taiwan.tick-borne ehrlichiosis and mite-borne scrub typhus represent important emerging zoonotic rickettsial diseases. although scrub typhus has been recognized by the taiwanese public health system, information on ehrlichial infections is scarce in taiwan. in this study, the risk of spread of ectoparasites on rodents through aerial and marine transportation was assessed in international and domestic harbors. here, we report the first systematic surveillance of seroprevalence against ehrlichia spp. in ...201626817445
rickettsia felis, an emerging flea-borne rickettsiosis.rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ...201627340613
rickettsial infections in southeast asia: implications for local populace and febrile returned travelers.rickettsial infections represent a major cause of non-malarial febrile illnesses among the residents of southeast asia and returned travelers from that region. there are several challenges in recognition, diagnosis, and management of rickettsioses endemic to southeast asia. this review focuses on the prevalent rickettsial infections, namely, murine typhus (rickettsia typhi), scrub typhus (orientia tsutsugamushi), and members of spotted fever group rickettsiae. information on epidemiology and reg ...201424957537
multilocus sequence typing and dna similarity analysis implicates that a borrelia valaisiana-related sp. isolated in japan is distinguishable from european b. valaisiana.lyme disease borrelia spp. are transmitted by ixodes ticks, and more than 10 species of borreliae have been identified around the world. recently, another borrelia sp. has been reported in asia (japan, korea, china, taiwan and thailand) as borrelia valaisiana-related sp. in the present study, we obtained and genetically characterized 19 b. valaisiana-related sp. strains from mammals and ticks. genetic analyses showed that the borrelia strains were distinct from b. valaisiana found in europe. mul ...201323665530
widespread rickettsia spp. infections in ticks (acari: ixodoidea) in taiwan.ticks are second to mosquitoes as the most important disease vectors, and recent decades have witnessed the emergence of many novel tick-borne rickettsial diseases, but systematic surveys of ticks and tick-borne rickettsioses are generally lacking in asia. we collected and identified ticks from small mammal hosts between 2006 and 2010 in different parts of taiwan. rickettsia spp. infections in ticks were identified by targeting ompb and glta genes with nested polymerase chain reaction. in total, ...201526336223
a preliminary field survey of ectoparasites of rodents in urban park, sarawak, malaysian borneo.a survey of ectoparasites was carried out during eco-zoonoses expedition in bukit aup jubilee park (bajp), sibu, sarawak, malaysian borneo from 5(th) to 9(th) june 2008. a total of nine individuals comprising two species of rodents were captured. the species of rodents screened for ectoparasites were sundamys muelleri and callosciurus notatus. four genera and six species of ectoparasites were collected, namely, ixodes granulatus, ixodes sp., laelaps sedlaceki, laelaps nuttalli, hoplopleura dissi ...201324189685
ectoparasites of small mammals in four localities of wildlife reserves in peninsular malaysia.field surveys of ectoparasites on rodents and scandents were conducted in four localities of wildlife reserves in peninsular malaysia from october 2008 to november 2009. a total of 16 animals comprising 5 species of hosts were caught and examined for ectoparasites. the hosts examined were maxomys rajah, maxomys whiteheadi, leopoldamys sabanus, lariscus insignis and tupaia glis. of these hosts, 9 genera, consisting of 14 species of ectoparasites were extracted. three species of ticks (ixodidae), ...201122299462
species identification of ixodes granulatus (acari: ixodidae) based on internal transcribed spacer 2 (its2) sequences.to investigate the genetic specificity of ixodes granulatus ticks collected from taiwan, the genetic identities and phylogenetic relationships were analyzed by comparing the sequences of the internal transcribed spacer 2 (its2) region obtained from 27 strains of ticks representing twelve species of ixodes. five major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granulatus ticks collected from taiwan and japan were g ...201121153754
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