| effect of bithionol against paragonimus ohirai and paragonimus miyazakii in diffusion chambers implanted intraperitoneally or subcutaneously in rats. | | 1976 | 1255365 |
| the localization of allergens of paragonimus westermani by pleural exudates from patients. | the allergens of the lung fluke paragonimus westermani were localized by indirect immunostaining in adult fluke sections using pleural exudates from 3 patients with p. westermani. immunostaining performed by using pleural exudate with the highest level of specific ige revealed that the p. westermani major allergen (or allergens) was located in the gut epithelium and luminal contents and that minor allergens were in the tegument and parenchyma. the antigens recognized by specific igg were located ... | 1991 | 1779297 |
| paragonimus ohirai: immunobiochemical characterization on the tegumental glycocalyx of excysted juvenile recognized by a monoclonal antibody. | the tegumental glycocalyx of excysted juvenile (ej) of paragonimus ohirai was immunobiochemically characterized using a monoclonal antibody (ms-mab). hplc gel filtration showed that the antigens detected by two-site elisa had a molecular weight of greater than or equal to 2 x 10(6) da (dextran marker). on reduced sds-page, the glycocalyx antigen retained in the stacking gel was cleaved into several much smaller antigens after pronase treatment. the antigenic activity of the glycocalyx was stable ... | 1991 | 1901799 |
| morphological features of spermatozoa of paragonimus ohirai (trematoda: platyhelminthes) examined by a silver nitrate staining technique. | the mature thread-like spermatozoon of paragonimus ohirai was shown to be composed of 7 distinct segments which have morphologically different characteristics, following staining with silver nitrate. the first segment (1s) is a needle-shaped structure with a perforatorium. the 2s is composed of two filaments loosely paired; 3s consists of a somewhat coarse thread (vestige of the cytoplasmic projection); 4s is composed of two flagella tightly paired; in 5s, two silver-positive filaments are indep ... | 1991 | 1945518 |
| fertilization and oogenesis of the lung fluke, paragonimus ohirai (trematoda:troglotrematidae). | | 1988 | 3225118 |
| induction of secondary ige antibody response in rats immunized with x-irradiated metacercariae of paragonimus ohirai. | rats reinfected with paragonimus ohirai (p.o.) elicited little secondary ige response to adult p.o. antigen. in contrast, rats immunized with x-irradiated (2-10 krad) metacercariae elicited not only a marked primary ige response comparable to that in normally infected rats, but also a marked secondary ige response after challenge infection. rats immunized with 2 krad x-irradiated metacercariae yielded a higher secondary ige response than with 5 or 10 krad irradiated ones, and elicited a secondar ... | 1988 | 3338861 |
| genetic variability and differentiation of natural populations in three japanese lung flukes, paragonimus ohirai, paragonimus iloktsuenensis and paragonimus sadoensis (digenea: troglotrematidae). | the genetic differences among 3 closely related species, paragonimus ohirai, paragonimus iloktsuenensis and paragonimus sadoensis, were assessed by electrophoretic analysis of allozymes. fifteen enzymes were analyzed using starch gel electrophoresis. seven of 18 loci were polymorphic. the most variable population (pos) was polymorphic at 6 of the loci (p = 0.333) with a heterozygosity h of 0.054. genetic distances between p. ohirai and p. iloktsuenensis, between p. ohirai and p. sadoensis, and b ... | 1986 | 3746563 |
| paragonimus ohirai: genetic control of tetrazolium oxidase isozymes. | the japanese lung fluke, paragonimus ohirai, has three electrophoretic variants: f, fs, and s of tetrazolium oxidase (ec 1.15.1.1). variant flukes were crossed in the laboratory. in both crosses, s x s and f x f, parental phenotypes appeared in all respective f1 progeny. in a cross of f x s, all f1 individuals derived from each parent showed the same phenotype (fs) indicating a heterozygote. on the other hand, from the cross of fs x s, 13 of fs and 11 s were observed from a parent (fs) while 2 f ... | 1985 | 4076386 |
| experimental studies on the intramolluscan development of an oriental lung fluke, paragonimus ohirai miyazaki, 1939. | | 1965 | 5295714 |
| on the infectivity of an oriental lung fluke, paragonimus ohirai miyazaki, 1939 to some gastropod molluscs, with remarks on that of other species of the genus in japan. a review. | | 1965 | 5326374 |
| kinetics and localization of parasite-specific ige in paragonimus ohirai-infected rats. | | 1982 | 7141778 |
| ige in paragonimus ohirai-infected rats: relationship between titer, migration route, and parasite age. | in rats infected with paragonimus ohirai (p.o.), ige response against the parasite was influenced by both the site of metacercarial inoculation and the age of the inoculated worms, whereas the iha response was not influenced by these variables. intraperitoneal infection and oral infection with 10 metacercariae induced a high level of p.o.-specific ige (maximum pca titer of 1:128 and 1:111, respectively). in contrast, intrapleural infection induced little ige (1:496, maximum titer). thus migratio ... | 1980 | 7391861 |
| cytochemical localization of cytochrome c oxidase activity in mitochondria in the tegument and tegumental and parenchymal cells of the trematodes echinostoma trivolvis, zygocotyle lunata, schistosoma mansoni, fasciola gigantica and paragonimus ohirai. | cytochrome c oxidase in the mitochondria of the tegument and tegumental and parenchymal cells was examined cytochemically in echinostoma trivolvis, zygocotyle lunata, schistosoma mansoni, fasciola gigantica and paragonimus ohirai, trematodes that inhabit different sites in their vertebrate hosts. clear differences in enzyme activity occurred in the mitochondria of these species, probably reflecting the different energy metabolisms of these worms. marked aerobic metabolism occurred in s. mansoni ... | 1995 | 8522764 |
| two types of parenchymal cells in the lung fluke paragonimus ohirai (digenea: troglotrematidae) characterized by the cytochemistry of their mitochondria. | morphology and respiratory function were studied in situ and in the isolated mitochondria of paragonimus ohirai. two types of parenchymal cells (i.e., pc1 and pc2 cells), whose mitochondria differ in terms of morphology and staining for cytochrome c oxidase activity, were found in fluke tissues. enzymatic and spectrophotometric analyses of the isolated mitochondria showed that fluke mitochondria possess both aerobic and anaerobic respiratory chains. these results suggest that there are two mitoc ... | 1996 | 8653591 |
| identification of a new fluke allergen identified by monoclonal ige antibodies for paragonimus miyazakii. | ige-producing hybridomas were acquired from fusing splenic cells to myeloma cells of balb/c mice infected with paragonimus miyazakii. with the use of the monoclonal antibody (mab)xige obtained, the localization of the allergen in p. miyazakii, as well as its molecular weight, was evaluated. the allergen was present in the gut epithelium and luminal contents of adult flukes. because this allergen was absent in the related species paragonimus westermani and paragonimus ohirai, we believe that it i ... | 1998 | 9576503 |
| development of the ovary and the female reproductive cells of the lung fluke, paragonimus ohirai (trematoda: troglotrematidae). | the ultrastructure of the ovary of paragonimus ohirai was investigated in different developmental stages of experimental infection in rats, from the metacercarial stage to the adult stage. the female reproductive cells were observed in order to understand the development of the ovary. during its development in the definitive host, the ovarian primordium and the ovary increased in size and cell number and underwent morphological changes. the blind end of the female genitalia was an undifferentiat ... | 1987 | 3572648 |
| metamorphosis of the excretory system of paragonimus ohirai (trematoda), with special reference to its functional significance. | the fine structure of the excretory system was studied in metacercariae and juveniles of paragonimus ohirai. the former were in vitro excysted, and the latter were collected from the abdominal cavity of a rat 24 hours postinfection. the terminal organs of the excretory system were composed of a flame cell and the first cell of a tubule. in the excysted metacercaria, there was no space between these cells to allow the passage of fluid. this suggests that the terminal organs of p. ohirai may be in ... | 1987 | 3430635 |
| effect of ionophores on in vitro excystment of paragonimus ohirai metacercariae. | ca2+ ionophores (a23187 and ionomycin) induced in vitro excystment of paragonimus ohirai metacercariae, whereas na+ ionophore (monensin) and k+ ionophores (nigericin and valinomycin) did not. the effect of a23187 on in vitro excystment was dependent on the concentration of the ionophore. a high concentration of 1 microm or more induced rapid excystment, but resulted in the death of metacercarial juveniles. the appropriate concentration of 0.2 microm induced in vitro excystment by 5 h after incub ... | 2001 | 11355686 |
| paragonimus ohirai: identification of nucleolar organizer regions (nors) and silver nitrate staining pattern in spermatogenesis. | the identification of nucleolus organizer regions (nors) and the qualitative description of silver nitrate staining patterns in spermatogenesis of paragonimus ohirai are herein reported. the nors of p. ohirai are located on the short arm of chromosome 4 and are associated with a large amount of constitutive heterochromatin. silver (ag) staining was detected from the interphase stage until the prometaphase stage of mitosis in spermatogonial cells but was absent at metaphase. on the other hand, du ... | 1988 | 3191960 |
| novel c-banding patterns in paragonimus ohirai (trematoda: platyhelminthes). | the c-banding pattern for the karyotype of paragonimus ohirai representing individuals in a new population is reported. the short arm of chromosome 4 consisted of a large pericentromeric proximal c-band block and euchromatic tip. this pattern has not been observed previously and is designated as type e. other new observations were: chromosome 5 was composed of pericentromeric heterochromatin, a lightly stained intercalary band at the middle portion of the short arm, and a lightly stained interst ... | 1989 | 2918436 |
| effect of bordetella pertussis adjuvant on parasite-specific ige response in paragonimus ohirai-infected rats. | parasite-specific ige response in rats infected with metacercariae of the lung fluke, paragonimus ohirai, was enhanced about 8 times by bordetella pertussis adjuvant. in rats infected by intraperitoneal transplantation of adult worms, the adjuvant markedly increased the usually low or absent parasite-specific ige response. the most effective time of the adjuvant administration was different between the two modes of infections. | 1988 | 2892799 |
| induction of resistance in oncomelania hupensis nosophora against schistosoma japonicum, but not against paragonimus ohirai, using irradiated miracidia. | oncomelania hupensis nosophora snails sensitized with x-irradiated schistosoma japonicum miracidia demonstrated resistance against a following challenge infection with non-irradiated homologous miracidia. the resistance in o. h. nosophora against s. japonicum was acquired within 1 day of sensitization, and it was strongest in a group challenged at an interval of 3 days. the resistance persisted for at least 4 weeks. histological examinations revealed amoebocyte accumulation around the challenged ... | 1989 | 2592137 |
| development of the excretory bladder of the lung fluke paragonimus ohirai (trematoda: troglotrematidae). | the excretory bladder of paragonimus ohirai was studied during development in rats. parasites were obtained at each development stage, and single worms were prepared for both transmission and scanning electron microscopy (sem). during early transformation (1, 2 days), excretory materials such as calcareous concretions and lipid droplets filled the bladder, and golgi complexes and various types of cytoplasmic bodies were abundant. the bladder epithelia of late transformation (3, 5 days) were char ... | 1990 | 2319421 |
| ultrastructure of mehlis' gland in the lung fluke, paragonimus ohirai (trematoda: troglotrematidae). | mehlis' gland of a digenetic trematode, paragonimus ohirai, is composed of two types of secretory cells, db and cb. the less abundant type (db) produces dense bodies, with the cytoplasm characterized by greatly distended cisternae of rough endoplasmic reticulum. the other type (cb) synthesizes clear, vesicular bodies. its cytoplasm contains numerous mitochondria, rough endoplasmic reticulum with narrow cisternae, and abundant golgi complexes. processes of the two cell types converge on the ootyp ... | 1991 | 1999841 |
| host-parasite relationships between paragonimus ohirai and various crabs. i. morphology of the metacercariae parasitic in sesarma (holometopus) dehaani and s. (h.) haematocheir. | | 1976 | 993576 |
| pyrimidine nucleotide biosynthesis in clonorchis sinensis and paragonimus ohirai. | | 1978 | 372107 |
| antibody responses to fluke cysteine proteinases in paragonimus- and fasciola-infected rats. | igg and igm antibody responses to fluke cysteine proteinases in paragonimus ohirai- and fasciola sp.-infected rats were followed by means of cystatin capture elisa using fluke excretory-secretory products for 10 weeks after infection. the specific igg antibodies were detectable at week 2 postinfection in all p. ohirai-infected and some fasciola-infected rats. levels of specific igg antibodies increased rapidly between week 2 and 6, and slightly thereafter, in both infected groups. from week 3, s ... | 1998 | 9765368 |
| molecular evidence for the synonymy of three species of paragonimus, p. ohirai miyazaki, 1939, p. iloktsuenensis chen, 1940 and p. sadoensis miyazaki et al., 1968 | the paragonimus ohirai group, named after p. ohirai miyazaki, 1939, consists of three nominal species. paragonimus iloktsuenensis chen, 1940 and p. sadoensis miyazaki et al., 1968, the other members of the group, were proposed primarily because of perceived differences in metacercarial morphology and/or host preferences. it has long been recognized that adults of the three were virtually indistinguishable. with the application of genetic techniques, it has become clear that the three forms can e ... | 1997 | 9445405 |
| ultrastructure of the ootype of the lung fluke paragonimus ohirai (digenea, troglotrematidae) in mated and single-infection worms. | the ootype of paragonimus ohirai was studied by both scanning and transmission electron microscopy. the ootype wall in 20-week-old worms from single infections was similar in its epithelial cell architecture and components to that of mated worms at 10 weeks postinfection. the lining epithelium consisted of a single layer of nucleated cells. the cytoplasm displayed a variety of organelles such as occasional golgi complexes, well-developed annulate lamellae, frequent lysosomes, abundant mitochondr ... | 1994 | 8073017 |
| excretory/secretory antigens of adult paragonimus ohirai recognized by a monoclonal antibody. | an igm monoclonal antibody (mab) that reacted with the gut epithelium and luminal contents of adult paragonimus ohirai was prepared from a mouse immunized with the excretory/secretory (es) products of the parasite. the mab reacted with the same sites not only of immature worms and newly excysted juveniles of p. ohirai, but also of adult worms of other paragonimus species, such as p. westermani and p. miyazakii. the mab did not react with adult antigens prepared from any other helminth parasites. ... | 1995 | 7657458 |
| c-band polymorphism in a japanese lung fluke paragonimus ohirai (trematoda; platyhelminthes). | | 1981 | 7319834 |
| ige in paragonimus ohirai-infected rats: effect of x-irradiation. | | 1982 | 7131201 |
| experimental infection of oncomelania quadrasi with paragonimus ohirai. | two hundred oncomelania quadrasi collected from leyte, philippines were exposed to infection with paragonimus ohirai, a rodent type lung fluke. in a group, each snail was exposed individually to 10 miracidia hatched from eggs which were brought from japan to the philippines. in another group, 100 snails were placed in a petri dish and p. ohirai miracidia were added to provide 10 per snail. the observations were made each successive week after exposure. all the snails examined were positive for t ... | 1984 | 6505784 |
| histochemical studies of glycosidase activity in juveniles and adults of the lung fluke paragonimus. | glycosidase activities in the adults and juveniles of the lung fluke paragonimus ohirai and p. westermani adults were demonstrated histochemically. for comparative studies, histochemical examination was also made on the adults of the liver fluke fasciola hepatica. the enzymes examined were n-acetyl-beta-glucosaminidase (ec 3.2.1.30), beta-glucuronidase (ec 3.2.1.31), beta-galactosidase (ec 3.2.1.23), alpha-glucosidase (ec 3.2.1.20) and beta-glucosidase (ec 3.2.1.21). the distribution of beta-glu ... | 1983 | 6220258 |
| evidence for metacercarial polymorphism in lung flukes, paragonimus ohirai and paragonimus iloktsuenensis. | cross breeding experiments between 2 species of lung fluke, paragonimus ohirai and paragonimus iloktsuenensis, were carried out using metacercarial characteristics as distinguishing markers. all metacercariae of f1 obtained were identical to those of p. ohirai. in the f2 and bf1 of f1 x p. iloktsuenensis, both the p. ohirai and p. iloktsuenensis types of metacercariae appeared. in the bf1 of f1 x p. ohirai, however, only the p. ohirai type metacercariae were produced. no intermediate type betwee ... | 1985 | 4093814 |
| the inheritance of enzyme variants of glutamic-oxaloacetic transaminase in paragonimus ohirai. | lung flukes, paragonimus ohirai, from kinosaki, japan, characterized by 3 electrophoretic variants, ff, fs and ss, of glutamic-oxaloacetic transaminase (got: ec 2.6.1.1) were crossed in the laboratory. in the case of a cross between fs and ss, a parent, fs, produced 9 ss and 16 fs clones in the offspring, numbers not significantly different from that expected (1:1) from mendelian inheritance. from the other cross fs x ff, 20 clones originating from the respective 20 miracidia were obtained from ... | 1985 | 3841198 |
| purification and properties of a thiol protease from lung fluke adult paragonimus ohirai. | the thiol protease was purified from adult paragonimus ohirai by alpha 1-antitrypsin-sepharose, sephadex g-75 and cm-cellulose, measuring its activities to hydrolyze hemoglobin and tosyl-l-lysine alpha-naphthyl-ester. the purified protease showed a single band on polyacrylamide disc gel isoelectrophoresis as zymogram with tos-lys-ne and also by protein staining, and its pi was found to be 6.4. the molecular weight was calculated to be 29,000 by gel filtration and 27,000 by sds-polyacrylamide gel ... | 1986 | 3514108 |
| ultrastructure of spermatozoa of the lung fluke, paragonimus ohirai (trematoda: troglotrematidae), in the seminal receptacle. | the seminal receptacle of paragonimus ohirai contains not only mature spermatozoa, but also atypical and degenerate ones, suggesting that abnormal spermatozoa are retained in this organ. the spermatozoon is of a parallel biflagellar type with cortical microtubules, consisting of the anterior region, first mitochondrial region, intermediate (amitochondrial) region, second mitochondrial region, posterior nuclear region (pnr) and tail region (tr). the first third of the spermatozoon exhibits typica ... | 1988 | 3418719 |
| schistosoma japonicum and paragonimus ohirai: antagonism between s. japonicum and p. ohirai in oncomelania nosophora. | antagonistic interactions between schistosoma japonicum and paragonimus ohirai were examined in the snail host, oncomelania nosophora. when p. ohirai-infected snails were exposed to s. japonicum miracidia at intervals of 4 to 18 weeks post-first exposure, only a few snails (0-7%) were found to be superinfected with s. japonicum sporocysts. sporocysts were fewer in number than those of single infected controls. mature s. japonicum cercariae were not observed. furthermore, when the snails were exa ... | 1988 | 3338543 |
| further studies on the effect of cyclosporin a on the course of paragonimus infection in rats. | paragonimus ohirai-infected rats were treated with cyclosporin a (cya) at different times during the course of infection. cya (5 x 80 mg/kg) affected the worm recovery, growth and maturation rates of p. ohirai with respect to control values. this tendency was most remarkable in animals treated 15 days and more after infection with cya (groups b, +15 to +19 days; c, +25 to +29; d, +35 to +39 and e, +45 to +49). in group a (0 to +4), however, the drug did not affect markedly the growth and maturat ... | 1989 | 2600414 |
| ultrastructure of the oviduct of the lung fluke, paragonimus ohirai (trematoda: troglotrematidae). | the oviduct of paragonimus ohirai is lined by an epithelium composed of cells that rest on a prominent basal lamina and exhibit extensive basal infoldings. apical surfaces of the epithelial cells are lamellated, and height and abundance of lamellae vary in different regions. cilia are confined to two separate areas and are variable with respect to their axonemal pattern. there are five principal regions of the oviduct: infundibular (ir), distal ciliary (dcr), preseminal receptacle (psr), proxima ... | 1990 | 2366242 |
| molecular identification of paragonimus ohirai and p. westermani from anhui province, china. | nucleotide sequences of the internal transcribed spacer 2 (its2) region were determined from seven adults of species paragonimus collected from jinde and xiuning counties, anhui province, china. among these, the nucleotide sequence obtained from one paragonimus adult (jinde county) was identical to the its2 sequence of p. ohirai previously reported. in order to confirm the result, partial regions of mitochondrial cytochrome c oxidase i (coi) and nadh dehydrogenase 1 (nd1) from the putative p. oh ... | 2000 | 11147039 |
| involvement of cysteine proteinases in excystment of paragonimus ohirai metacercariae induced by sodium cholate and a23187. | the involvement of intrinsic proteinases in the excystment of paragonimus ohirai metacercariae was studied in in vitro excystment induced by sodium (na) cholate, a bile salt and a23187, a ca2+ ionophore. the effects of various proteinase inhibitors on the in vitro excystment were examined and similar inhibitory profiles were obtained. benzyloxycarbonyl-l-leucyl-l-leucinal (z-leu-leu-h), a cysteine proteinase inhibitor and 4-(2-aminoethyl)-benzenesulfonyl fluoride (pefabloc sc), a serine proteina ... | 2003 | 12590660 |
| ecological analysis on the mechanism of the host preference of miracidia of paragonimus ohirai miyazaki, 1939 in natural condition. | | 1961 | 14454614 |
| host preference of miracidia of paragonimus ohirai miyazaki, 1939 among three species of snails of the genus assiminea. | | 1961 | 14454615 |
| paragonimus ohirai metacercariae in crabs collected along the arakawa river in tokyo, japan. | brackish water crabs infected with paragonimus ohirai metacercariae have been reported in various regions in japan. however, infected crabs have not been identified in tokyo. we therefore collected the crab, chiromantes dehaani, between august 2002 and july 2003 from 12 locations along the arakawa river that flows through tokyo. of the 922 captured crabs, 177 (19%) from 6 locations were infected with paragonimus metacercariae. the prevalence of metacercariae at these 6 locations ranged from 5 to ... | 2004 | 15353842 |
| effects of blockers of ca2+ channels and other ion channels on in vitro excystment of paragonimus ohirai metacercariae induced by sodium cholate. | the inhibitory effects of various ion channel blockers were examined on in vitro excystment of paragonimus ohirai metacercariae induced by a bile salt, sodium cholate. at a concentration of 10 microm, bepridil, a non-selective ca(2+) channel blocker, completely inhibited in vitro excystment, whereas tea, lidocaine, and r(+)-iaa-94, channel blockers against k(+), na(+) and cl(-) ions, respectively, benzamil, an na(+)/h(+) and na(+)/ca(2+) ion exchanger blocker, and r(+)-dioa, a [k(+), cl(-)] cotr ... | 2004 | 15449179 |
| paragonimus westermani and some rare intestinal trematodes recovered from raccoon dogs (nyctereutes procyonoides viverrinus) introduced recently on yakushima island, japan. | helminth parasites were collected from 9 raccoon dogs (nyctereutes procyonoides viverrinus) and 2 japanese weasels (mustela itatsi sho) on yakushima island, japan. the former carnivore was introduced to this world natural heritage area presumably within the last two decades, expanding its population thence, although detailed process(es) of the introduction is unknown. the collected trematodes from raccoon dogs included the triploid form of paragonimus westermani, brachylaima tokudai, maritrema e ... | 2006 | 16891780 |
| evaluation of the mgl method to detect paragonimus eggs and its improvement. | dog feces containing 500 paragonimus westermani eggs per gram were examined by the medical general laboratory (mgl), the simple sedimentation (ss), and the army medical school iii (ams iii) methods. the number of eggs per gram of feces (epg) obtained by the mgl method was 17.2 and was significantly lower than those obtained by the ss method (324.0) and the ams iii method (505.6). when isolated p. westermani eggs were processed by the mgl method and four layers (ether, ether-fecal, formalin layer ... | 2015 | 26243572 |
| identification and characterization of major allergens in excretory/secretory products of the worm paragonimus ohirai. | allergens present in the excretory/secretory (es) products of adult paragonimus ohirai were biochemically identified. immunoblot analysis using sera from p. ohirai-infected rats revealed only two allergens to be major proteins in the es products, with apparent molecular masses (m(r)) of 27 and 29 kd. as the es products contained a high proportion of acidic and neutral cysteine proteinases, we examined whether or not the allergens and the cysteine proteinases were identical. the acidic and neutra ... | 2002 | 12119500 |
| effects of l-type ca2+ channel antagonists on in vitro excystment of paragonimus ohirai metacercariae induced by sodium cholate. | the inhibitory effects of l-type ca2+ channel antagonists on na cholate-induced in vitro excystment (ciie) of paragonimus ohirai metacercariae were studied. at concentrations of 10 microm, nicardipine and nimodipine inhibited ciie completely and by approximately 92%, respectively. nitrendipine and (+/-)-verapamil inhibited ciie by about one half and one third, respectively. nifedipine and diltiazem did not inhibit ciie significantly. at higher concentrations, nitrendipine at 20 microm completely ... | 2006 | 16565818 |