the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria.a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ...1979471028
microbial transformations of some monoterpenoids and sesquiterpenoids.the absolute configurations of fragrances, flavours and drugs are often important for their special properties. the growing interest of organic chemists in chiral synthons has stimulated work on biotransformations, for which readily available and inexpensive compounds can be used as substrates. microbial transformations of 1-menthenes like gamma-terpinene, alpha-terpinene, limonene and alpha-phellandrene give the corresponding 1,2-trans-diols with high stereospecificity. because of the volatilit ...19853848377
corynespora cassiicola leaf spot of pawpaw (carica papaya l.) in nigeria.leaf spot of pawpaw is hereby reported for the first time in nigeria. the symptom is characterized by a papery center surrounded by a yellow halo. the causal organism is corynespora cassiicola. ripe fruits and abaxial surfaces of the leaves were significantly more susceptible to infection than unripe fruits and adaxial surfaces of leaves. growth and sporulation of the fungus on several media was investigated. the organism grew faster on malt-extract agar (mea) derived media and slowest on potato ...199910560625
additions to the mycobiota of the weed lantana camara (verbenaceae) in southeastern brazil.a sequel to the work of systematic surveying the mycobiota of lantana camara, aimed at finding potential biocontrol agents, was carried out during 1995-1996 covering part of its centre of origin in brazil (state of minas gerais). fifty-eight sampling sites, representing the four main climatic types in the state of minas gerais, were surveyed. additional ad hoc collections were made in the states of bahia, espírito santo, parana, rio de janeiro and são paulo. fifteen fungal species were recorded ...200111554581
isolation of scopoletin from leaves of hevea brasiliensis and the effect of scopoletin on pathogens of h. brasiliensis.scopoletin (7-hydroxy-6-methoxy coumarin) which inhibited the conidial germination of corynespora cassiicola was isolated from the uninfected mature leaves of hevea brasiliensis. scopoletin was not detected in uninfected immature rubber leaves. the immature leaves produced scopoletin after being infected with c. cassiicola. the concentration of scopoletin in infected leaves was higher than in uninfected mature leaves. scopoletin also inhibited the conidial germination of other fungal pathogens o ...200212014480
gene expression analysis in cucumber leaves primed by root colonization with pseudomonas chlororaphis o6 upon challenge-inoculation with corynespora cassiicola.root colonization by pseudomonas chlororaphis o6, a non-pathogenic rhizobacterium, induced systemic resistance in cucumber against target leaf spot caused by corynespora cassiicola. a cdna library was constructed using mrna extracted from cucumber leaves 12 h after inoculation with c. cassiicola, using plants colonized by o6. to identify genes involved in o6-mediated induced systemic resistance (isr), we employed a subtractive hybridization method using mrnas extracted from pathogen-challenged c ...200415045660
purification and characterization of cassiicolin, the toxin produced by corynespora cassiicola, causal agent of the leaf fall disease of rubber tree.cassiicolin, a phytotoxin produced by the necrotrophic fungus corynespora cassiicola, was purified to homogeneity from a rubber tree isolate. the optimized protocol involves reverse phase chromatography followed by size exclusion chromatography, with monitoring of the toxicity on detached rubber tree leaves. cassiicolin appeared to be a peptide composed of 27 amino acids, glycosylated on the second residue, with a n-terminal pyroglutamic acid and 6 cysteines involved in disulfide bonds. its mole ...200717113837
structural analysis of cassiicolin, a host-selective protein toxin from corynespora cassiicola.cassiicolin is a host-selective toxin (hst) produced by the fungus corynespora cassiicola (strain ccp). it is responsible for the corynespora leaf fall (clf) disease, which is among the main pathologies affecting rubber tree (hevea brasiliensis). working on purified cassiicolin and using electron microscopy, we have demonstrated that this 27-residue o-glycosylated protein is able to induce cellular damages identical to those induced by the fungus on rubber tree leaves and displays the same host ...200717234212
molecular characterization and diagnosis of qoi resistance in cucumber and eggplant fungal pathogens.abstract the molecular mechanism of qoi fungicide resistance was studied using isolates of cucumber corynespora leaf spot fungus (corynespora cassiicola) and the eggplant leaf mold (mycovellosiella nattrassii). in both pathogens, a mutation at position 143 from glycine to alanine (g143a) was detected in the cytochrome b gene that encodes for the fungicide-targeted protein. moreover, the nucleotide sequence at amino acid position 143 was converted from ggt or gga in sensitive (wild-type) to gct o ...200718943516
galactinol is a signaling component of the induced systemic resistance caused by pseudomonas chlororaphis o6 root colonization.root colonization by pseudomonas chlororaphis o6 in cucumber elicited an induced systemic resistance (isr) against corynespora cassiicola. in order to gain insight into o6-mediated isr, a suppressive subtractive hybridization technique was applied and resulted in the isolation of a cucumber galactinol synthase (csgols1) gene. the transcriptional level of csgols1 and the resultant galactinol content showed an increase several hours earlier under o6 treatment than in the water control plants follo ...200818986260
rare or remarkable microfungi from oaxaca (south mexico)--part ii.microfungi were collected in southern mexico in the vicinity of puerto escondido, oaxaca in 2007. in 2006, samples were gathered from acacia myrmecophytes [(remarkable microfungi from oaxaca of acacia species) part i]. in the present investigation [part ii], we collected microfungi from different parts of a variety of wild and cultivated higher plants belonging to the families anacardiaceae, caricaceae, fabaceae, moraceae, and nyctaginacae. the microfungi found here live as parasites or saprophy ...200819226752
modeling the three-dimensional structures of an unbound single-chain variable fragment (scfv) and its hypothetical complex with a corynespora cassiicola toxin, cassiicolin.rubber trees infected with a host-specific cassiicolin toxin often experience considerable leaf fall, which in turn results in loss of crop productivity. it was recently revealed that cassiicolin-specific single-chain variable fragments (scfv) can successfully reduce the toxic effects of cassiicolin. however, the detailed mechanism of antibody action remains poorly understood. the primary sequence of the newly sequenced cassiicolin-specific scfv was highly homologous to several members of single ...201020232097
[identification of cucumber disease using hyperspectral imaging and discriminate analysis].hyperspectral imaging (400-720 nm) and discriminate analysis were investigated for the detection of normal and diseased cucumber leaf samples with powdery mildew (sphaerotheca fuliginea), angular leaf spot (pseudomopnas syringae), downy mildew (pseudoperonospora cubensis), and brown spot (corynespora cassiicola). a hyperspectral imaging system was es tablished to acquire and pre-process leaf images, as well as to extract leaf spectral properties. owing to the complexity of the original spectral ...201020672633
lack of cross-resistance to a novel succinate dehydrogenase inhibitor, fluopyram, in highly boscalid-resistant isolates of corynespora cassiicola and podosphaera xanthii.recently in japan, isolates resistant to boscalid, a succinate dehydrogenase inhibitor (sdhi), have been detected in corynespora cassiicola (burk. & curt.) wei and podosphaera xanthii (castaggne) braun & shishkoff, the pathogens causing corynespora leaf spot and powdery mildew disease on cucumber, respectively. resistant isolates of c. cassiicola are widely distributed and represent a serious problem in disease control at present. novel sdhi fungicides, including fluopyram, are now under develop ...201121394880
[Early detection of corynespora spot on cucumber leaves based on FTIR spectroscopy].Fourier transform infrared spectroscopy (FTIR) technique was applied in the early detection of corynespora spot on cucumber leaves while the symptom had not appeared. The cucumber leaves were inoculated with Corynespora cassiicola. By observing the changes in the FTIR spectra of infected cucumber leaves at various times of post-infection, three sensitive bands, 1 735, 1 545 and 1 240 cm(-1) were selected for the identification of cucumber corynespora leaf spot. According to the peak areas at the ...201121847920
characterization of a cassiicolin-encoding gene from corynespora cassiicola, pathogen of rubber tree (hevea brasiliensis).corynespora leaf fall (clf) is a major disease of rubber tree (hevea brasiliensis) caused by the ascomycota corynespora cassiicola. here we describe the cloning and characterization of a gene encoding cassiicolin (cas), a glycosylated cystein-rich small secreted protein (ssp) identified as a potential clf disease effector in rubber tree. three isolates with contrasted levels of aggressiveness were analyzed comparatively. the cassiicolin gene was detected - and the toxin successfully purified - f ...201122325885
corynespora cassiicola, a new agent of maduromycetoma. 19695388128
Transformation of structurally diverse steroidal analogues by the fungus Corynespora cassiicola CBS 161.60 results in generation of 8ß-monohydroxylated metabolites with evidence in favour of 8ß-hydroxylation through inverted binding in the 9a-hydroxylase.Corynespora cassiicola has a unique but unexplored ability amongst fungi, in that it can hydroxylate 17a-hydroxyprogesterone at the highly hindered C-8 position of the steroid nucleus. In order to gain greater understanding of the mechanistic basis and capability of the 8ß-hydroxylase we have transformed a range of structurally diverse androgens and progestogens with this organism. This has revealed that both steroid types can be hydroxylated at the 8ß-position. The collective data has demonstra ...201122009141
design, synthesis, biological activities, and 3d-qsar of new n,n'-diacylhydrazines containing 2-(2,4-dichlorophenoxy)propane moiety.a series of new n,n'-diacylhydrazine derivatives were synthesized efficiently under microwave irradiation. their structures were characterized by (1) h nmr, ms, and elemental analysis. various biological activities of these compounds were tested. most of them exhibited higher herbicidal activities against dicotyledonous weeds than monocotyledonous weeds. in addition, favorable in vivo fungicidal activities were also found of these compounds against cladosporium cucumerinum, corynespora cassiicol ...201121816005
phaeohyphomycosis caused by a plant pathogen, corynespora cassiicola.we report a case of subcutaneous phaeohyphomycosis caused by corynespora cassiicola. molecular identification of this pathogen on grasses confirms that it may be involved in human infection, as previously reported once in pre-molecular literature. in vitro antifungal susceptibility data of the strain are provided. the patient was successfully treated with oral terbinafine with topical povidone iodine in accordance with the results obtained through in vitro susceptibility testing.201121281061
synthesis and antifungal activities of new pyrazole derivatives via 1,3-dipolar cycloaddition reaction.a series of cycloadducts--pyrazoles via 1,3-dipolar cycloaddition reactions of generated nitrilimines with n-(4-chloro-2-fluorophenyl)maleimide were described. the novel compounds synthesized were characterized by (1)h nmr, ms, and elemental analysis. the fungicidal tests showed that most of the title compounds exhibit significant fungicidal activities against corynespora cassiicola.201020486935
molecular and pathogenic variation identified among isolates of corynespora cassiicola.pathogenicity tests and inter simple sequence repeat (issr) molecular fingerprinting markers were utilized to analyze 24 corynespora cassiicola isolates obtained from a lot of hevea clones grown in most rubber nurseries and a few plantations in china. the c. cassiicola isolates were collected from hainan and yunnan provinces, china, from 2006 to 2008. the assay of 24 c. cassiicola isolates on detached leaves of four different hevea rubber clones (genotypes pr 107, dafeng 95, rrim 600, and reyan ...200918841502
genetic variation in corynespora cassiicola: a possible relationship between host origin and virulence.genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was investigated using rapd-pcr analysis. five genetic groups were identified using rapd-pcr profiles generated by eight random primers. results indicate that there is a significant genetic variation among c. cassiicola isolates collected from different host plants. these results should facilitate the development of rubber clones with enhanced res ...200312884953
biotransformations of the cardiovascular drugs mexrenone and canrenone.microbial transformation studies of the cardiovascular drugs mexrenone (1) and canrenone (2) were conducted. thirty-nine biotransformations of mexrenone and 84 biotransformations of canrenone were analyzed. metabolism of the substrate was observed in the majority of these cases. several monohydroxylated derivatives were detected by hplc-ms-uv and subsequently identified. two new mexrenone derivatives, 11alpha- (3) and 12beta-hydroxymexrenone (4), and the known metabolite 6beta-hydroxymexrenone ( ...200312662091
isolation and identification of 5-hydroxyl-5-methyl-2-hexenoic acid from actinoplanes sp. hbdn08 with antifungal activity.a bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antifungal activity from actinoplanes sp. hbdn08. the structure of the antifungal metabolite was elucidated as 5-hydroxyl-5-methyl-2-hexenoic acid on the basis of spectral analysis. this compound showed strong in vitro antifungal activity against botrytis cinerea, cladosporium cucumerinum and corynespora cassiicola, with an ic(50) of 32.45, 27.17, and 30.66 mg/l, respectively; ...201020584599
synthesis and fungicidal activity of aryl carbamic acid-5-aryl-2-furanmethyl ester.chitin, a major structural component of insect cuticle and fungus cell wall but absent in plants and vertebrates, is regarded as a safe and selective target for pest control agents. chitin synthesis inhibitors (csis) have been well-known as insect growth regulators (igrs) but rarely found as fungicides in agriculture. to find novel csis with good activity, benzoylphenylurea, a typical kind of csis, was chosen as the lead compound and 26 novel aryl carbamic acid-5-aryl-2-furanmethyl esters were d ...201020151651
[cucumber diseases diagnosis using multispectral imaging technique].for a reliable diagnosis of plant diseases and insect pests, spectroscopy analysis technique and mutispectral imaging technique are proposed to diagnose five cucumber diseases, namely trichothecium roseum, sphaerotheca fuliginea, cladosporium cucumerinum, corynespora cassiicola and pseudoperonospora cubensis. in the experiment, the cucumbers' multispectral images of 14 visible lights channels, near infrared channel and panchromatic channel were captured using narrow-band multispectral imaging sy ...200919445229
fungal biotransformation of (+/-)-linalool.the biotransformation of (+/-)-linalool was investigated by screening 19 fungi. product accumulation was enhanced by substrate feeding and, for the first time, lilac aldehydes and lilac alcohols were identified as fungal biotransformation byproduct using spme-gc-ms headspace analysis. aspergillus niger dsm 821, botrytis cinerea 5901/02, and b. cinerea 02/fbii/2.1 produced different isomers of lilac aldehyde and lilac alcohol from linalool via 8-hydroxylinalool as postulated intermediate. linaloo ...200818426215
antifungal constituent from gordonia dassanayakei.the hexane extract of the stem bark of gordonia dassanayakei showed high antifungal activity against plant pathogenic fungi curvularia sp., colletotrichum gloeosporioides, rhizoctonia solani, corynespora cassiicola, and fusarium sp. a compound responsible for the antifungal activity was isolated and identified as 3-formyl-2,4-dihydroxy-6-methylbenzoic acid 3-hydroxy-4-(methoxycarbonyl)-2,5-dimethylphenyl ester (1).200111429257
isolation of antifungal compounds from gardenia jasminoides.gardenia jasminoides e. (rubiaceae) methanol extracts showed the highest level of antifungal activity against pleurotus ostreatus, a wood-rotting fungus, compared to five other methanol plants extracts; [thuja orientalis l. (cupressaceae), datura innoxia (solanaceae), ligustrum japonicum t. (oleaceae), juniperus chinensis var. procumbens (cupressaceae) and mallotus japonica m. (euphorbiaceae)] and selected for further analysis. two antifungal compounds were isolated from n-butanol and ethyl acet ...200919817121
morphological and inter simple sequence repeat (issr) markers analyses of corynespora cassiicola isolates from rubber plantations in malaysia.morphological features and inter simple sequence repeat (issr) polymorphism were employed to analyse 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in malaysia. the c. cassiicola isolates used in this study were collected from several states in malaysia from 1998 to 2005. the morphology of the isolates was characteristic of that previously described for c. cassiicola. variations in colony and conidial morphology were observed not only among ...200818568417
[horticultural plant diseases multispectral classification using combined classified methods].the research on multispectral data disposal is getting more and more attention with the development of multispectral technique, capturing data ability and application of multispectral technique in agriculture practice. in the present paper, a cultivated plant cucumber' familiar disease (trichothecium roseum, sphaerotheca fuliginea, cladosporium cucumerinum, corynespora cassiicola, pseudoperonospora cubensis) is the research objects. the cucumber leaves multispectral images of 14 visible light ch ...201020384138
subcutaneous infection caused by corynespora cassiicola, a plant pathogen.we describe a 69-year-old female farmer with diabetes mellitus who developed subcutaneous infection due to a plant pathogen, corynespora cassiicola. the organism was identified based on characteristic morphotypes and confirmed by sequence analysis of the internal transcribed spacer (its) regions. the patient was treated successfully with amphotericin b therapy.201019925826
host specialization and phylogenetic diversity of corynespora cassiicola.the fungus corynespora cassiicola is primarily found in the tropics and subtropics, and is widely diverse in substrate utilization and host association. isolate characterization within c. cassiicola was undertaken to investigate how genetic diversity correlates with host specificity, growth rate, and geographic distribution. c. cassiicola isolates were collected from 68 different plant species in american samoa, brazil, malaysia, and micronesia, and florida, mississippi, and tennessee within the ...200919671003
aromatase inhibitory, radical scavenging, and antioxidant activities of depsidones and diaryl ethers from the endophytic fungus corynespora cassiicola l36.isolation of a broth extract of the endophytic fungus corynespora cassiicola l36 afforded three compounds, corynesidones a (1) and b (3), and corynether a (5), together with a known diaryl ether 7. compounds 1, 3, 5, and 7 were relatively non-toxic against cancer cells, and inactive toward normal cell line, mrc-5. corynesidone b (3) exhibited potent radical scavenging activity in the dpph assay, whose activity was comparable to ascorbic acid. based on the orac assay, compounds 1, 3, 5, and 7 sho ...200919230943
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