| identification of streptococci from greek kasseri cheese and description of streptococcus macedonicus sp. nov. | taxonomic studies were performed on some streptococcus-like organisms isolated from naturally fermented greek kasseri cheese. by sds-page analysis of whole-cell proteins the group was found to be quite different from streptococcus thermophilus. comparative 16s and 23s rrna sequence analyses showed that the isolates represent a new species within the genus streptococcus, where they are most closely related to the streptococcus bovis cluster. on the basis of these phylogenetic results and some phe ... | 1998 | 9731293 |
| comparison between streptococcus macedonicus and streptococcus waius strains and reclassification of streptococcus waius (flint et at. 1999) as streptococcus macedonicus (tsakalidou et al. 1998). | two species of dairy streptococci, streptococcus waius and streptococcus macedonicus, were originally characterized by 16s-23s intergenic spacer sequence analysis, random amplified polymorphic dna fingerprinting, pfge analysis and dna-dna reassociation experiments. all genetic data suggested that s. waius strains belong to the previously described species s. macedonicus. likewise, the phenotypic characterization showed that strains of s. macedonicus and s. waius were highly related and easily di ... | 2002 | 12054262 |
| taxonomic dissection of the streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (soda) sequences: reclassification of 'streptococcus infantarius subsp. coli' as streptococcus lutetiensis sp. nov. and of streptococcus bovis biotype 11.2 as streptococcus pasteurianus sp. nov. | the taxonomic dissection of the streptococcus bovis-streptococcus equinus group was carried out upon obtaining sequences for the manganese-dependent superoxide dismutase gene (soda) of the type strains of s. bovis, streptococcus caprinus, s. equinus, streptococcus gallolyticus, streptococcus infantarius, streptococcus macedonicus and streptococcus waius. the soda sequences of 29 streptococcal strains of animal and human origin that were related to s. bovis were also sequenced. a phylogenetic ana ... | 2002 | 12148636 |
| macedocin, a food-grade lantibiotic produced by streptococcus macedonicus aca-dc 198. | streptococcus macedonicus aca-dc 198, a strain isolated from greek kasseri cheese, produces a food-grade lantibiotic named macedocin. macedocin has a molecular mass of 2,794.76 +/- 0.42 da, as determined by electrospray mass spectrometry. partial n-terminal sequence analysis revealed 22 amino acid residues that correspond with the amino acid sequence of the lantibiotics sa-ff22 and sa-m49, both of which were isolated from the pathogen streptococcus pyogenes. macedocin inhibits a broad spectrum o ... | 2002 | 12450808 |
| rapid detection and identification of streptococcus macedonicus by species-specific pcr and dna hybridisation. | the aim of this study was to develop a simple and specific method for the rapid detection and identification of streptococcus macedonicus. the method was based on polymerase chain reaction (pcr) using species-specific primers derived from the 16s rrna gene. specific identification was proven on seven s. macedonicus strains, while 16 strains belonging to different lactic acid bacteria species were tested negative. the pcr assay was capable of detecting 100 pg of s. macedonicus dna, and it was als ... | 2003 | 12485749 |
| microbial and physiological characterization of weakly amylolytic but fast-growing lactic acid bacteria: a functional role in supporting microbial diversity in pozol, a mexican fermented maize beverage. | pozol is an acid beverage obtained from the natural fermentation of nixtamal (heat- and alkali-treated maize) dough. the concentration of mono- and disaccharides from maize is reduced during nixtamalization, so that starch is the main carbohydrate available for lactic acid fermentation. in order to provide some basis to understand the role of amylolytic lactic acid bacteria (alab) in this fermented food, their diversity and physiological characteristics were determined. forty amylolytic strains ... | 2003 | 12902217 |
| indigenous raw milk microbiota influences the bacterial development in traditional cheese from an alpine natural park. | nostrano di primiero is a 6-month ripened cheese produced from raw milk collected in the paneveggio-pale di san martino natural park area in the italian dolomites. in summer, this cheese is made using milk collected from two different areas, passo rolle and vanoi, in the paneveggio natural park. during the experiment, the milk from the two areas was separately processed, and cheeses were made in the same cheese factory using the same technological process. the microbiota of raw milk and cheeses ... | 2004 | 15109791 |
| diversity of lactic acid bacteria isolated from aoc salers cheese. | the objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk salers cheeses at the species and strain levels. the characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: rep-pcr, species and genus specific amplifications and the sequence analysis of 16s rdna for strain typing and taxonomic identification. the strains belonged to lactobacillus plantar ... | 2004 | 15190953 |
| streptococcus macedonicus aca-dc 198 produces the lantibiotic, macedocin, at temperature and ph conditions that prevail during cheese manufacture. | streptococcus macedonicus aca-dc 198, a natural cheese isolate, produces the anticlostridial bacteriocin, macedocin. bacteriocin activity was detected from the mid-exponential growth phase and remained constant during the stationary phase. a secondary model was setup to describe the influence of temperature (20-45 degrees c) and ph (5.1-6.9) on cell growth of and bacteriocin production by s. macedonicus aca-dc 198 during in vitro laboratory fermentations. the optimum temperature for bacteriocin ... | 2006 | 16288813 |
| occurrence of streptococcus macedonicus in italian cheeses. | a new approach for the detection and enumeration of streptococcus macedonicus in cheese was developed. the method which is based on a first screening of cheeses by a pcr assay specific for s. macedonicus followed by plating positive samples on a differential medium (sm medium) was applied to 51 samples derived from pdo and traditional italian cheeses. streptococcus macedonicus was found in 16 of the 51 samples examined in the present work. with the exclusion of an asiago cheese sample in which v ... | 2006 | 16842361 |
| use of artificial neural networks and a gamma-concept-based approach to model growth of and bacteriocin production by streptococcus macedonicus aca-dc 198 under simulated conditions of kasseri cheese production. | growth of and bacteriocin production by streptococcus macedonicus aca-dc 198 were assessed and modeled under conditions simulating kasseri cheese production. controlled fermentations were performed in milk supplemented with yeast extract at different combinations of temperature (25, 40, and 55 degrees c), constant ph (phs 5 and 6), and added nacl (at concentrations of 0, 2, and 4%, wt/vol). the data obtained were used to construct two types of predictive models, namely, a modeling approach based ... | 2007 | 17158625 |
| characterization of the gene cluster involved in the biosynthesis of macedocin, the lantibiotic produced by streptococcus macedonicus. | streptococcus macedonicus aca-dc 198, a food-grade isolate from naturally fermented greek kasseri cheese, produces a lantibiotic named macedocin that has been previously purified and characterized. in the present study, a 15,171 bp region in the s. macedonicus aca-dc 198 chromosome, containing the biosynthetic gene cluster of macedocin, has been sequenced. this region consists of 10 orfs, which correspond to the genes (mcd genes) involved in macedocin biosynthesis, regulation and immunity. the m ... | 2007 | 17581139 |
| lactobacillus fermentum aca-dc 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (tnbs)-induced colitis and salmonella infection in murine models. | lactobacillus fermentum aca-dc 179, lactobacillus plantarum aca-dc 287 and streptococcus macedonicus aca-dc 198 were studied for their probiotic potential. firstly, strains were screened for antimicrobial activity towards a broad range of target strains, including lactic acid bacteria, food spoilage and pathogenic bacteria. l. fermentum aca-dc 179 was active against five streptococci, including the two pathogenic strains streptococcus oralis lmg 14532t and streptococcus pneumoniae lmg 14545t. s. ... | 2008 | 18077037 |
| bacteriocin st91km, produced by streptococcus gallolyticus subsp. macedonicus st91km, is a narrow-spectrum peptide active against bacteria associated with mastitis in dairy cattle. | streptococcus gallolyticus subsp. macedonicus st91km produces a bacteriocin (macedocin st91km) active against streptococcus agalactiae, streptococcus dysgalactiae subsp. dysgalactiae, streptococcus uberis, staphylococcus aureus, and staphylococcus epidermidis. macedocin st91km is, according to tricine-sds page, between 2.0 and 2.5 kda in size. antimicrobial activity remained unchanged after 2 h of incubation at ph 2.0-10.0 and after 100 min at 100 degrees c. the peptide was inactivated after 20 ... | 2008 | 18641698 |
| identification of streptococcus gallolyticus subsp. macedonicus as the etiological agent in a case of culture-negative multivalve infective endocarditis by 16s rdna pcr analysis of resected valvular tissue. | today, pcr using broad-range primers is being used increasingly to detect pathogens from resected heart valves. herein is described the first case of multivalve infective endocarditis where 16s rdna pcr was used to detect a single pathogen from two affected valves in a 61-year-old man. triple heart valve replacement was required despite six weeks of appropriate antimicrobial therapy. the organism was confirmed as streptococcus gallolyticus subsp. macedonicus, a member of the 's. equinus/s. bovis ... | 2008 | 18980096 |
| milk protein fragments induce the biosynthesis of macedocin, the lantibiotic produced by streptococcus macedonicus aca-dc 198. | the aim of the present work was to study the mode of the induction of the biosynthesis of macedocin, the lantibiotic produced by streptococcus macedonicus aca-dc 198. macedocin was produced when the strain was grown in milk but not in mrs or m17 broth. no autoinduction mechanism was observed. production did not depend on the presence of lactose or galactose in the culture medium or on a coculture of the producer strain with macedocin-sensitive or macedocin-resistant strains. induction seemed to ... | 2010 | 20023075 |
| characterization of a bacteriocin produced by lactobacillus sakei r1333 isolated from smoked salmon. | strain r1333, isolated from commercially available smoked salmon, was identified as lactobacillus sakei based on biochemical tests, sugar fermentation reactions (api 50 chl), pcr with species-specific primers and sequencing of the 16s rrna gene. strain r1333 produces a 3811 kda class iia bacteriocin, active against streptococcus caprinus, streptococcus macedonicus, streptococcus spp., l. sakei, lactococcus lactis subsp. lactis, listeria innocua, listeria ivanovii subsp. ivanovii and listeria mon ... | 2011 | 20152920 |
| analysis of bacterial communities of traditional fermented west african cereal foods using culture independent methods. | in this study, the microbial composition of kunu-zaki and ogi, two popular foods in nigeria produced after natural, uncontrolled fermentation of cereals, was assessed by culture-independent molecular profiling methods. in particular, pcr-denaturing gradient gel electrophoresis and construction of 16s rrna gene clone libraries revealed the presence of diverse bacterial communities. dna sequencing of the highly variable v3 region of the 16s rrna genes obtained from pcr-dgge fingerprints identified ... | 2011 | 21272950 |
| extension of tosèla cheese shelf-life using non-starter lactic acid bacteria. | six strains of non-starter lactic acid bacteria (nslab) were used to extend the shelf-life of the fresh cheese tosèla manufactured with pasteurised cows' milk. the acidification kinetics of three lactobacillus paracasei, one lactobacillus rhamnosus and two streptococcus macedonicus were studied in synthetic milk medium. lb. paracasei ndp78 and ndp88 and s. macedonicus ndp1 and pb14-1 showed an interesting acidifying capacity and were further characterised for growth in uht milk and production of ... | 2011 | 21569930 |
| short communication: characterization of microflora in mexican chihuahua cheese. | this work was performed to identify the bacterial species present in 10 chihuahua cheeses obtained from commercial producers in mexico using 16s rrna gene analysis. as expected, some of the agar media initially used for isolation were not very selective, supporting the growth of several unrelated bacterial species. sequence analysis identified potential pathogens, including escherichia coli and staphylococcus aureus, in all raw milk samples and 2 pasteurized milk samples. streptococcus thermophi ... | 2011 | 21700016 |
| culture-independent detection of microorganisms in traditional slovakian bryndza cheese. | in order to investigate the microflora of slovakian bryndza cheese (a cheese containing unpasteurized or pasteurized ewes' milk component) by a culture-independent method, dna was extracted directly from 7 bryndza samples and analysed by an innovative method. using the universal prokaryotic and fungal primers, ribosomal dna internal transcribed spacer (its) regions with variable length were amplified. the standard universal reverse primer l1 aligning to bacterial 23s rdna was found unsuitable fo ... | 2011 | 21849217 |
| suicin 3908, a new lantibiotic produced by a strain of streptococcus suis serotype 2 isolated from a healthy carrier pig. | while streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. we hypothesized that s. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic s. suis strains. two of ten s. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic s. suis isolates. ... | 2015 | 25659110 |
| a perspective study of koumiss microbiome by metagenomics analysis based on single-cell amplification technique. | koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. in the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. this approach aimed at detecting the low-abundant bacteria in the koumiss. briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. then, three diluted bacterial suspensions were randomly picked for further study. by ... | 2017 | 28223973 |
| a large factory-scale application of selected autochthonous lactic acid bacteria for pdo pecorino siciliano cheese production. | the main hypothesis of this study was that the autochthonous lactic acid bacteria (lab) selected for their dairy traits are able to stabilize the production of pdo (protected denomination of origin) pecorino siciliano cheese, preserving its typicality. the experimental plan included the application of a multi-strain lactic acid bacteria (lab) culture, composed of starter (lactococcus lactis subsp. lactis cag4 and cag37) and non starter (enterococcus faecalis psl71, lactococcus garviae psl67 and ... | 2016 | 27375245 |
| the microfloras and sensory profiles of selected protected designation of origin italian cheeses. | approximately 39 italian cheeses carry protected designation of origin (pdo) status. these cheeses differ in their manufacturing technology and the microbial flora which comprise the finished products. the evolution of lactic microflora in cheeses with pdo status is of particular interest because the biochemical activities of these organisms participate in cheesemaking and may play an acknowledged role in the development of organoleptic characteristics during ripening. nonstarter lactic acid bac ... | 2014 | 26082116 |
| molecular characterization of lactic acid bacteria and in situ amylase expression during traditional fermentation of cereal foods. | lactic acid bacteria play an important role in traditional fermented foods consumed in different countries. study of their taxonomic structure and diversity is necessary for starter culture selection, improved safety and nutritional enhancement. to achieve these objectives, microbial genomic typing methods were used to study genetic differences of autochthonous bacteria and their distribution in two traditional african fermented cereal foods. a total of 85 predominant bacterial species were isol ... | 2012 | 22608231 |
| purification and characterization of suicin 65, a novel class i type b lantibiotic produced by streptococcus suis. | bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent streptococcus suis (serotype 2). in this study, a novel bacteriocin produced by s. suis has been identified and characterized. the producing strain s. suis 65 (serotype 2) was fo ... | 2015 | 26709705 |
| comparative genome analysis of streptococcus infantarius subsp. infantarius cj18, an african fermented camel milk isolate with adaptations to dairy environment. | streptococcus infantarius subsp. infantarius (sii) belongs to the streptococcus bovis/streptococcus equinus complex associated with several human and animal infections. sii is a predominant bacterium in spontaneously fermented milk products in africa. the genome sequence of sii strain cj18 was compared with that of other streptococcus species to identify dairy adaptations including genome decay such as in streptococcus thermophilus, traits for its competitiveness in spontaneous milk fermentation ... | 2013 | 23521820 |
| incidence of bacteriocins produced by food-related lactic acid bacteria active towards oral pathogens. | in the present study we investigated the incidence of bacteriocins produced by 236 lactic acid bacteria (lab) food isolates against pathogenic or opportunistic pathogenic oral bacteria. this set of lab contained several strains (≥17%) producing bacteriocins active against food-related bacteria. interestingly only streptococcus macedonicus aca-dc 198 was able to inhibit the growth of streptococcus oralis, streptococcus sanguinis and streptococcus gordonii, while lactobacillus fermentum aca-dc 179 ... | 2013 | 23443163 |
| macedovicin, the second food-grade lantibiotic produced by streptococcus macedonicus aca-dc 198. | streptococcus macedonicus aca-dc 198 was found to produce a second lantibiotic named macedovicin in addition to macedocin. macedovicin was purified to homogeneity and mass spectrometric analysis identified a peptide of approximately 3.4 kda. partial n-terminal sequence analysis and tandem mass spectrometry revealed that macedovicin was identical to bovicin hj50 and thermophilin 1277 produced by streptococcus bovis and streptococcus thermophilus, respectively. macedovicin inhibits a broad spectru ... | 2013 | 23122510 |
| Short communication: Technological and genotypic comparison between Streptococcus macedonicus and Streptococcus thermophilus strains coming from the same dairy environment. | The species Streptococcus thermophilus is widely used for the preparation of several dairy products, and its technological contribution is clear. On the other hand, although Streptococcus macedonicus was first described more than 10 yr ago and, despite the scientific interest around this issue, the exact role of Strep. macedonicus in cheese making has yet to be clarified. In this study, 121 strains belonging to both species and isolated from the same dairy environment were genetically characteri ... | 2011 | 22118078 |
| screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts. | the selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. the aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, bifidobacterium longum and bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. ... | 2011 | 21619939 |
| in vitro and in vivo safety evaluation of the bacteriocin producer streptococcus macedonicus aca-dc 198. | streptococcus macedonicus aca-dc 198, a bacteriocin producer isolated from greek kasseri cheese, was used in a series of in vitro and in vivo experiments in order to evaluate its pathogenic potential. the strain was examined in vitro for haemolytic activity, antibiotic resistance and presence of pathogenicity genes encountered in streptococcus pyogenes. subsequently, the strain was orally administered to mice (8.9 log cfu daily), continuously over a period of 12 weeks, in order to ascertain the ... | 2009 | 19515446 |
| rna arbitrarily primed pcr and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of streptococcus macedonicus. | we have previously reported that an acid tolerance response (atr) can be induced in streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic ph, or acid habituation, as well as at stationary phase. here, we compared the transcriptional profiles of these epigenetic phenotypes, by rna arbitrarily primed pcr (rap-pcr), and their whole-cell chemical compositions, by fourier transform infrared spectroscopy (ft-ir). rap-pcr fingerprints revealed significan ... | 2008 | 18689510 |
| synthesis of oligosaccharide fragments corresponding to the exopolysaccharide released by streptococcus macedonicus sc 136. | hexa-, penta- and tetrasaccharide fragments related to the repeating unit of the exopolysaccharide secreted by streptococcus macedonicus sc 136 were synthesized in a very efficient manner involving minimum number of steps. a general glycosylation condition has been applied for all glycosylation steps and yields were excellent. | 2008 | 17659442 |
| acid tolerance of streptococcus macedonicus as assessed by flow cytometry and single-cell sorting. | an in situ flow cytometric viability assay employing carboxyfluorescein diacetate and propidium iodide was used to identify streptococcus macedonicus acid tolerance phenotypes. the logarithmic-phase acid tolerance response (l-atr) was evident when cells were (i) left to autoacidify unbuffered medium, (ii) transiently exposed to nonlethal acidic ph, or (iii) systematically grown under suboptimal acidic conditions (acid habituation). stationary-phase atr was also detected; this phenotype was gradu ... | 2007 | 17098924 |
| rapid assessment of the physiological status of streptococcus macedonicus by flow cytometry and fluorescence probes. | flow cytometry in combination with fluorescence probes was applied to rapidly assess the physiological status of streptococcus macedonicus aca-dc 198, a newly described member of the lactic acid bacteria group with technologically important features (e.g. lantibiotic production). a sonication procedure was developed for disaggregating typical streptococci chains in order to optimize cell preparations for single cell analysis. single stained live and dead populations of s. macedonicus cells were ... | 2006 | 16934355 |
| reappraisal of the taxonomy of the streptococcus bovis/streptococcus equinus complex and related species: description of streptococcus gallolyticus subsp. gallolyticus subsp. nov., s. gallolyticus subsp. macedonicus subsp. nov. and s. gallolyticus subsp. pasteurianus subsp. nov. | 'streptococcus bovis/streptococcus equinus' is a large bacterial complex including different species frequently isolated from infections of humans (streptococcus gallolyticus, streptococcus infantarius) or animals (s. bovis, s. equinus, streptococcus alactolyticus). the separation of s. bovis into three different biotypes has been partially correlated with genetic differentiation. in addition, recent advances in bacterial phylogeny have led to the inclusion of streptococcus macedonicus and strep ... | 2003 | 12807180 |
| structure and properties of the exopolysaccharide produced by streptococcus macedonicus sc136. | streptococcus macedonicus is a gram positive lactic acid bacterium that is part of the starter flora present in greek sheep and goat cheeses. the s. macedonicus sc136 strain produces a high-molecular-mass, highly texturizing exopolysaccharide composed of d-glucose, d-galactose, and n-acetyl-d-glucosamine in the molar ratio of 3:2:1. the structure of the exopolysaccharide produced by s. macedonicus sc136 was determined by chemical analysis, mass spectrometry, and nuclear magnetic resonance spectr ... | 2001 | 11287400 |
| biochemical properties of streptococcus macedonicus strains isolated from greek kasseri cheese. | a total of 32 streptococcus macedonicus strains, isolated from greek kasseri cheese, were screened for biochemical properties of technological importance in milk fermentation processing, such as acid production, proteolytic and lipolytic activity, citrate metabolism, exopolysaccharide production, antimicrobial activity and biogenic amines production. all strains were found to be moderate acidifiers in milk. only four strains could hydrolyse milk casein, while 11 strains showed lipolytic activity ... | 2000 | 10792542 |
| streptococcus waius sp. nov., a thermophilic streptococcus from a biofilm. | thermophilic streptococci were isolated from biofilms on stainless steel samples exposed to pasteurized skimmed milk and from dairy products from a dairy manufacturing plant. the phenotypic characters of these isolates were distinct from those of other thermophilic streptococci of dairy origin (streptococcus thermophilus and streptococcus bovis). genotypic data [restriction endonuclease analysis, ribotyping, random amplified polymorphic dna (rapd) profiles, dna-dna hybridization and g + c conten ... | 1999 | 10319500 |
| whole-genome sequence of streptococcus macedonicus strain 33mo, isolated from the curd of morlacco cheese in the veneto region (italy). | a genetic characterization of streptococcus macedonicus is important to better understand the characteristics of this lactic acid bacterium, frequently detected in fermented food bacteria communities. this report presents the draft genome sequence description of strain 33mo, the first publicly available genome sequence of an italian s. macedonicus isolate. | 2014 | 25103758 |
| whole-genome sequences of three streptococcus macedonicus strains isolated from italian cheeses in the veneto region. | we report here the genome sequences of three streptococcus macedonicus strains isolated from different cheeses in the veneto region of italy. the presented data aim at increasing the scarce genomic information available for this species, which is frequently encountered in fermented foods and appears to be a promising technological microorganism. | 2017 | 29242223 |
| bacteriocin-like inhibitory substance (blis) activity of streptococcus macedonicus mbf10-2 and its synergistic action in combination with antibiotics. | to characterize the bacteriocin-like inhibitory-substances (blis) activity of streptococcus macedonicus mbf10-2 [named blis(mbf10-2)], a bacteriocinogenic strain isolated from an indonesian tofu byproduct. | 2017 | 29268969 |
| engineered strains of streptococcus macedonicus towards an osmotic stress resistant phenotype retain their ability to produce the bacteriocin macedocin under hyperosmotic conditions. | streptococcus macedonicus aca-dc 198 produces the bacteriocin macedocin in milk only under low nacl concentrations (<1.0%w/v). the thermosensitive plasmid pgh9:iss1 was employed to generate osmotic stress resistant (osmr) mutants of s. macedonicus. three osmr mutants showing integration of the vector in unique chromosomal sites were identified and the disrupted loci were characterized. interestingly, the mutants were able to grow and to produce macedocin at considerably higher concentrations of ... | 2015 | 26319319 |
| whole-genome sequence of the cheese isolate streptococcus macedonicus 679. | it is well recognized that streptococcus macedonicus can populate artisanal fermented foods, especially those of dairy origin. however, the safety of s. macedonicus remains to be established. here, we present the whole-genome sequence of strain 679, which was isolated from a french uncooked semihard cheese made with cow milk. | 2016 | 27660795 |
| periimplant bacteria associated with different transmucosal designs or smoking habits. | this study is to compare periimplant microbiota associated with implant transmucosal designs or smoking habits. | 2015 | 25706261 |
| characterization of teta-like gene encoding for a major facilitator superfamily efflux pump in streptococcus thermophilus. | efflux pumps are membrane proteins involved in the active extrusion of a wide range of structurally dissimilar substrates from cells. a multidrug efflux pump named teta belonging to the major facilitator superfamily (mfs) of transporters was identified in the streptococcus thermophilus dsm 20617(t) genome. the teta-like gene was found in the genomes of a number of s. thermophilus strains sequenced to date and in streptococcus macedonicus aca-dc 198, suggesting a possible horizontal gene transfer ... | 2014 | 24766488 |
| prevalence and comparison of streptococcus infantarius subsp. infantarius and streptococcus gallolyticus subsp. macedonicus in raw and fermented dairy products from east and west africa. | streptococcus infantarius subsp. infantarius (sii) and streptococcus gallolyticus subsp. macedonicus are members of the streptococcus bovis/streptococcus equinus complex (sbsec) associated with human infections. sbsec-related endocarditis was furthermore associated with rural residency in southern europe. sbsec members are increasingly isolated as predominant species from fermented dairy products in europe, asia and africa. african variants of sii displayed dairy adaptations to lactose metabolis ... | 2013 | 24131584 |
| phylogenetic, epidemiological and functional analyses of the streptococcus bovis/streptococcus equinus complex through an overarching mlst scheme. | the streptococcus bovis/streptococcus equinus complex (sbsec) comprises seven (sub)species classified as human and animal commensals, emerging opportunistic pathogens and food fermentative organisms. changing taxonomy, shared habitats, natural competence and evidence for horizontal gene transfer pose difficulties for determining their phylogeny, epidemiology and virulence mechanisms. thus, novel phylogenetic and functional classifications are required. an sbsec overarching multi locus sequence t ... | 2016 | 27329036 |
| mode of action and in vitro susceptibility of mastitis pathogens to macedocin st91km and preparation of a teat seal containing the bacteriocin. | mastitis is considered to be the most economically costly disease affecting the dairy industry. regular dosage of animals with antibiotics, including use of prophylactic concentrations, may select for resistant strains. the purpose of this study was to determine the mode of action of a new bacteriocin (macedocin st91km), to evaluate the antimicrobial resistance of mastitis pathogens to antibiotics commonly used in treatment remedies, and to introduce the possible use of an alternative antimicrob ... | 2010 | 24031473 |
| comparative genomics of the dairy isolate streptococcus macedonicus aca-dc 198 against related members of the streptococcus bovis/streptococcus equinus complex. | within the genus streptococcus, only streptococcus thermophilus is used as a starter culture in food fermentations. streptococcus macedonicus though, which belongs to the streptococcus bovis/streptococcus equinus complex (sbsec), is also frequently isolated from fermented foods mainly of dairy origin. members of the sbsec have been implicated in human endocarditis and colon cancer. here we compare the genome sequence of the dairy isolate s. macedonicus aca-dc 198 to the other sbsec genomes in or ... | 2014 | 24713045 |
| acquisition through horizontal gene transfer of plasmid psma198 by streptococcus macedonicus aca-dc 198 points towards the dairy origin of the species. | streptococcus macedonicus is an intriguing streptococcal species whose most frequent source of isolation is fermented foods similarly to streptococcus thermophilus. however, s. macedonicus is closely related to commensal opportunistic pathogens of the streptococcus bovis/streptococcus equinus complex. | 2015 | 25584532 |
| complete genome sequence of the dairy isolate streptococcus macedonicus aca-dc 198. | the species streptococcus macedonicus is associated with the food environment, especially with fermented dairy products. here we present the complete 2.1-mb genome sequence of strain aca-dc 198, which was isolated from naturally fermented greek kasseri cheese. | 2012 | 22408241 |