| isolation and characterization of cr(vi)-reducing actinomycetes from estuarine sediments. | bioremediation technologies have strong potential use in the less costly and more environmentally friendly removal of highly toxic hexavalent-chromium (cr(vi)) compared with physicochemical technologies. several cr(vi)-reducing bacteria have been isolated; however, there are few studies on cr(vi)-resistant and cr(vi)-reducing actinomycetes. in this study, cr(vi)-reducing actinomycetes were screened from estuarine, marine, and terrestrial samples on the basis of cr(vi)-resistant and cr(vi)-reduci ... | 2015 | 25672321 |
| glucose kinases from streptomyces peucetius var. caesius. | glucose kinases (glks) are enzymes of the glycolytic pathway involved in glucose phosphorylation. these enzymes can use various phosphoryl donors such as atp, adp, and polyphosphate. in several streptomycetes, atp-glucose kinase (atp-glk) has been widely studied and regarded as the main glucose phosphorylating enzyme and is likely a regulatory protein in carbon catabolite repression. in cell extracts from the doxorubicin overproducing strain streptomyces peucetius var. caesius, grown in glucose, ... | 2014 | 24687748 |
| cloning of the gene encoding an endo-acting pectate lyase from streptomyces thermocarboxydus. | an endo-acting family 3 pectate lyase (pl i) gene from streptomyces thermocarboxydus was cloned and expressed in escherichia coli. the deduced pl i sequence had highest similarity to a putative pectate lyase of s. coelicolor. recombinant pl i had significantly lower specific activity, probably a result of incorrect folding, as indicated by circular dichroism spectra analysis. | 2010 | 20139595 |
| effect of microbial activity on the mobility of chromium in soils. | the effect of microbial activity on the chemical state of chromium, in a contaminated soil located in the rhĵne-alpes region (france), has been investigated. this soil contained 4,700 mg kg(-1) cr, with about 40% present in the soluble hexavalent form. indigenous microbial activity was found to significantly reduce cr(vi) to the less mobile form (iii) when the soil was incubated at 30 degrees c in an aqueous medium containing glucose and nutrients. a cr(vi)-reducing strain of streptomyces thermo ... | 2002 | 12003148 |
| streptomyces thermocarboxydovorans sp. nov. and streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. | four moderately thermophilic, carboxydotrophic streptomycetes were the subject of a comparative taxonomic investigation designed to establish their taxonomic relationships. almost complete sequences of the 16s rrna genes of the test strains were determined following the isolation and direct sequencing of the amplified genes. the resultant nucleotide sequences were aligned with the sequences of previously studied streptomycetes, and phylogenetic trees generated by using the neighbour-joining, fit ... | 1998 | 9542077 |
| case of keratitis caused by streptomyces thermocarboxydus. | we describe a case of keratitis caused by streptomyces thermocarboxydus and report the usefulness of molecular analysis in identifying the exact species of streptomyces. a 50-year-old man was diagnosed with keratitis caused by streptomyces sp. which was identified as s. thermocarboxydus by sequencing the 16s rdna. he had no history of trauma or systemic diseases. he was initially treated with topical beta-lactams and fluoroquinolones, and systemic beta-lactams but the keratitis did not improve. ... | 2014 | 24462427 |
| sugar ester synthesis by thermostable lipase from streptomyces thermocarboxydus me168. | the extracellular lipase from streptomyces thermocarboxydus me168 was purified to 9.5-fold with 20% yield, following concentration by acetone precipitation, ion exchange chromatography (resource q) and gel filtration chromatography (superdex 200), respectively. the purified enzyme had an apparent molecular mass of 21 kda by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). the n-terminal sequence of the lipase was asdfddqilg and was different from most other reported lipase. ... | 2012 | 22434352 |
| a novel xylanase with tolerance to ethanol, salt, protease, sds, heat, and alkali from actinomycete lechevalieria sp. hj3. | a xylanase-coding gene (xynahj3, 1,104 bp) was cloned from lechevalieria sp. hj3 harbored in a saline soil sampled from heijing town, aka the "town of salt", on the famous "silk route of the south". the gene encodes a 367-residue polypeptide (xynahj3) with the highest identity of 74.0 % with the endoxylanase from streptomyces thermocarboxydus hy-15. the coding sequence of the mature protein (without the predicted signal peptide from m1 to s22) of xynahj3 was expressed in escherichia coli bl21 (d ... | 2012 | 22430498 |