| isolation of lectins from hemolymph of decapod crustaceans by adsorption on formalinized erythrocytes. | hemolymph of decapod crustaceans contains lectins of important specificity. an isolation procedure, based on adsorption of hemolymph lectins on red blood cells (rbc) fixed with formaldehyde, is described. hemolymph is let to clot for 3 h at 22-28 degrees c (rt) and for 24 h at 5 degrees c; centrifuged at 13000 g for 30 min; filtered through 5-microm filters; diluted with an equal volume of 50 mm nacl, 100 mm cacl(2); supplemented with protease as well as phenoloxidase inhibitors; centrifuged at ... | 2000 | 10889281 |
| isolation and characterization of a non sex-specific lipoprotein from hemolymph of fresh water crab potamon potamios. | a non sex-specific high density lipoprotein (hdl) was isolated from the hemolymph of male fresh water crab potamon potamios. antiserum against male lipoprotein precipitated a lipoprotein from ovigerous and non ovigerous females. the lipoprotein was isolated by a combination of kbr density gradient ultracentrifugation and gel filtration on bio-gel a-1.5m. two yellow bands with hydrated density of 1.08 and 1.10 g x ml-1 were observed following density gradient ultracentrifugation. native polyacryl ... | 2006 | 1418681 |
| primary structures of decapod crustacean metallothioneins with special emphasis on freshwater and semi-terrestrial species. | cadmium injections induced only a single form of metallothionein (mt) in the midgut gland of potamon potamios, whereas the same treatment induced two isoforms in astacus astacus. the only difference between the two latter isoforms was that one had an extra n-terminal methionine residue. mt from p. potamios showed structural differences from other decapod crustacean mts. it contained a gly-thr motif at positions 8 and 8a, which had previously been found only in certain vertebrate and molluscan mt ... | 1996 | 8921011 |