| molecular evidence that the asexual industrial fungus trichoderma reesei is a clonal derivative of the ascomycete hypocrea jecorina. | the relationship of the important cellulase producing asexual fungus trichoderma reesei to its putative teleomorphic (sexual) ancestor hypocrea jecorina and other species of the trichoderma sect. longibrachiatum was studied by pcr-fingerprinting and sequence analyses of the nuclear ribosomal dna region containing the internal transcribed spacers (its-1 and its-2) and the 5.8s rrna gene. the differences in the corresponding its sequences allowed a grouping of anamorphic (asexual) species of trich ... | 1996 | 8755548 |
| molecular reidentification of human pathogenic trichoderma isolates as trichoderma longibrachiatum and trichoderma citrinoviride. | several species of the well-known saprophytic genus trichoderma have been identified as the cause of infections in immunosuppressed humans. because the differentiation and identification of trichoderma species based on morphological characters only, is very difficult, two molecular approaches were applied for species identification. six human pathogenic trichoderma isolates were investigated by pcr-fingerprinting and analysis of ribosomal dna internal transcribed spacer (its) sequences and compa ... | 1999 | 10200931 |
| mycoflora and mycotoxins in brazilian black pepper, white pepper and brazil nuts. | a wide range of field and storage fungi were isolated from black pepper, white pepper and brazil nut kernels from amazonia. a total of 42 species were isolated from both peppers. aspergillus flavus and a. niger were isolated more frequently from black than from white pepper. other potential mycotoxigenic species isolated included: a. ochraceus, a. tamarii, a. versicolor, emericella nidulans and chaetomium globosum, penicillium brevicompactum, p. citrinum, p. islandicum and p. glabrum. species is ... | 2000 | 11229375 |
| identification of trichoderma strains by image analysis of hplc chromatograms. | forty-four trichoderma strains from water-damaged building materials or indoor dust were classified with chromatographic image analysis on full chromatographic matrices obtained by high performance liquid chromatography with uv detection of culture extracts. the classes were compared with morphological identification and rdna sequence data, and for each class all strains were of the same identity. with all three techniques each strain--except one--was identified as the same species. these strain ... | 2001 | 11583856 |
| antimicrobial activity of ergokonin a from trichoderma longibrachiatum. | natural fungal products were screened for antifungal compounds. the mode of action of one of the hits found and the taxonomy of the producing organism were analysed. | 2001 | 11722657 |
| immunoglobulin g antibodies against indoor dampness-related microbes and adult-onset asthma: a population-based incident case-control study. | immunoglobulin g (igg) antibodies against microbes related to indoor dampness problems have been used as potential biomarkers of fungal exposure in clinical investigations. there is limited information on their relation to asthma. we conducted a population-based incident case-control study to assess the risk of asthma in relation to specific igg antibodies to eight dampness-related microbes: aspergillus fumigatus, a. versicolor, cladosporium cladosporioides, fusarium oxysporum, sporobolomyces sa ... | 2002 | 12100029 |
| structural and biochemical studies of gh family 12 cellulases: improved thermal stability, and ligand complexes. | in this review we will describe how we have gathered structural and biochemical information from several homologous cellulases from one class of glycoside hydrolases (gh family 12), and used this information within the framework of a protein-engineering program for the design of new variants of these enzymes. these variants have been characterized to identify some of the positions and the types of mutations in the enzymes that are responsible for some of the biochemical differences in thermal st ... | 2004 | 15950056 |
| nonfatal trichoderma citrinoviride pneumonia in an acute myeloid leukemia patient. | | 2008 | 18180924 |
| citrantifidiene and citrantifidiol: bioactive metabolites produced by trichoderma citrinoviride with potential antifeedant activity toward aphids. | two novel metabolites with potential antifeedant activity were isolated from cultures of the fungus trichoderma citrinoviride strain item 4484 grown in solid-state fermentation on sterile rice kernels. the producing strain was identified at species level by sequence analysis of the internal transcribed spacer regions its-1 and its-2 of the nuclear rdna and a fragment of the translation elongation factor gene tef-1alpha. fractionation by column chromatography and tlc of the culture organic extrac ... | 2008 | 18435538 |
| development of a mutant of trichoderma citrinoviride for enhanced production of cellulases. | considering importance of a microbial strain capable of increased cellulases production and insensitive to catabolite repression for industrial use, we have developed a mutant strain of trichoderma citrinoviride by multiple exposures to ems and ethidium bromide. the mutant produced 0.63, 3.12, 8.22 and 1.94 iu ml(-1) fpase, endoglucanase, beta-glucosidase and cellobiase, respectively. these levels were, respectively, 2.14, 2.10, 4.09 and 1.73 fold higher than those in parent strain. glucose (upt ... | 2009 | 18951016 |
| bisorbicillinoids produced by the fungus trichoderma citrinoviride affect feeding preference of the aphid schizaphis graminum. | we report the effects of some bisorbicillinoids isolated from biomass of the fungus trichoderma citrinoviride on settling and feeding preference of the aphid schizaphis graminum. purification of the fungal metabolites was carried out by a combination of column chromatography and thin-layer chromatography using direct and reverse phases. chemical identification was performed by spectroscopic methods including nuclear magnetic resonance and mass spectrometry. the identified bisorbicillinoids appea ... | 2009 | 19418099 |
| occurrence and characterization of peptaibols from trichoderma citrinoviride, an endophytic fungus of cork oak, using electrospray ionization quadrupole time-of-flight mass spectrometry. | a cork oak endophytic strain of trichoderma citrinoviride, previously selected for its antagonistic potential against various fungal pathogens involved in oak decline, was screened for the production of bioactive secondary metabolites. from liquid culture a mixture of polypeptide antibiotics (peptaibols) belonging to the paracelsin family was isolated and characterized. this peptide mixture was purified by column chromatography and preparative tlc on silica gel, and separated by analytical hplc. ... | 2009 | 19574303 |
| cytotoxic activities of endophytic fungi isolated from the endangered, chinese endemic species dysosma pleiantha. | eleven strains of endophytic fungi which habitat in an endangered, chinese endemic medicinal plant, dysosma pleiantha (hance) woodson, were isolated and tested for their cytotoxic activity using the brine shrimp lethality bioassay. six isolates were found to exhibit some cytotoxic activity. extracts of f1273, f1276, and f1280, which were identified as trichoderma citrinoviride, chaetomium globosum and ascomycete sp., in particular, showed most potent activity with lc50 values of 4.86, 7.71, and ... | 2009 | 19791503 |
| exposure assessment of lovastatin in pu-erh tea. | this paper reports the results of an extensive survey on the levels of lovastatin in pu-erh tea samples. the microbial source of lovastatin was assessed by testing the ability of fungi with higher isolation frequency in the pu-erh tea samples to produce lovastatin on czapek yeast extract agar (cya). lovastatin was not detected in any of the raw pu-erh tea samples without storage but was found in almost all the ripe pu-erh tea samples, with lovastatin contents ranging from 20.61 ng/gdw to 226.38 ... | 2013 | 23587710 |
| trichocitrin, a new fusicoccane diterpene from the marine brown alga-endophytic fungus trichoderma citrinoviride cf-27. | one new diterpene, trichocitrin (1), and four known secondary metabolites, nafuredin (2), 5-hydroxy-2,3-dimethyl-7-methoxychromone (3), 24-methylenecycloartanol (4) and citrostadienol (5), were isolated from the culture of marine brown alga-endophytic trichoderma citrinoviride cf-27. trichocitrin (1) represents the first trichoderma-derived and furan-bearing fusicoccane diterpene, and its structure and relative configuration were identified by analysis of 1d/2d nmr and mass spectroscopic data. c ... | 2016 | 26728965 |
| enhanced reduction of phenol content and toxicity in olive mill wastewaters by a newly isolated strain of coriolopsis gallica. | the search for novel microorganisms able to degrade olive mill wastewaters (omw) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. in this work, the possibility of reducing the phenolic content of omw using new isolates of fungal strains (coriolopsis gallica, bjerkandera adusta, trametes versicolor, trichoderma citrinoviride, phanerochaete chrysosporium, gloeophyllum trabeum, trametes trogii, and fusarium solani) was inv ... | 2014 | 23979847 |
| citrinovirin with a new norditerpene skeleton from the marine algicolous fungus trichoderma citrinoviride. | citrinovirin (1), a novel norditerpene with an unprecedented carbon skeleton along with three known compounds, cyclonerodiol (2), 3-(2-hydroxypropyl)-4-(hexa-2e,4e-dien-6-yl)furan-2(5h)-one (3), and 5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone (4), was isolated from the culture of a marine brown alga-endophytic strain (cf-27) of trichoderma citrinoviride. the structure and relative configuration of 1 were identified by spectroscopic methods, including 1d/2d nmr and ms. its absolute confi ... | 2016 | 27612543 |
| trichoderma species occurring on wood with decay symptoms in mountain forests in central europe: genetic and enzymatic characterization. | the aim of this study was to explore the species diversity of trichoderma obtained from samples of wood collected in the forests of the gorce mountains (location a), karkonosze mountains (location b) and tatra mountains (location c) in central europe and to examine the cellulolytic and xylanolytic activity of these species as an expression of their probable role in wood decay processes. the present study has led to the identification of the following species and species complex: trichoderma atro ... | 2016 | 26586561 |
| the fungus trichoderma regulates submerged conidiation using the steroid pregnenolone. | in previous work, we evolved a population of trichoderma citrinoviride in liquid cultures to speed up its asexual development cycle. the evolved population, called t-6, formed conidia 3 times sooner and in >1000-fold greater numbers. here, we identify the steroid pregnenolone as a molecular signal for this different behavior. media in which the ancestral t. citrinoviride population was grown (called ancestral spent media) contained a submerged conidiation inhibitor. growing the evolved populatio ... | 2016 | 27413801 |
| long chain alcohols produced by trichoderma citrinoviride have phagodeterrent activity against the bird cherry-oat aphid rhopalosiphum padi. | in this study we report the effects of fungal metabolites isolated from cultures of the fungus trichoderma citrinoviride item 4484 on the feeding preference of the aphid rhopalosiphum padi, a major pest of cereal crops. different phagodeterrent metabolites were purified by a combination of direct and reverse phase column chromatography and thin-layer chromatography. chemical investigations, by spectroscopic and chemical methods, led to the identification of different long chain primary alcohols ... | 2016 | 27014220 |
| experimental evolution of trichoderma citrinoviride for faster deconstruction of cellulose. | engineering faster cellulose deconstruction is difficult because it is a complex, cooperative, multi-enzyme process. here we use experimental evolution to select for populations of trichoderma citrinoviride that deconstruct up to five-fold more cellulose. ten replicate populations of t. citrinoviride were selected for growth on filter paper by serial culture. after 125 periods of growth and transfer to fresh media, the filter paper deconstruction increased an average of 2.5 fold. two populations ... | 2016 | 26820897 |
| a rapid, one step molecular identification of trichoderma citrinoviride and trichoderma reesei. | trichoderma species are widely used as production hosts for industrial enzymes. identification of trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. such demands can be fulfilled by a brief method which enables confirmation of strain identity. this co ... | 2015 | 25752236 |
| [antimicrobial activity of substances produced by trichoderma citrinoviride strain vkpm f-1228: optimization of cultivation and assessment of spectrum of individual peptaibols]. | the trichoderma citrinoviride vkpm f-1228 strain produces a complex of peptide-based antibiotics with antibacterial and antimycotic action. synthesis of peptaibols is closely related to the conidiogenesis in the culture. the optimal procedure of the strain cultivation for production of peptaibols is stationary growing for 14 days at a temperature of 28 degrees c and ph 7.5 followed by formation of a dense mycelium film on the modified saburo medium containing 30 gr/l of glucose and 12.5 gr/l of ... | 2015 | 27141640 |
| growth inhibition of the cyanobacterium microcystis aeruginosa and degradation of its microcystin toxins by the fungus trichoderma citrinoviride. | harmful cyanobacterial blooms are recognized as a rapidly expanding global problem that threatens human and ecosystem health. many bacterial strains have been reported as possible agents for inhibiting and controlling these blooms. however, such algicidal activity is largely unexplored for fungi. in this study, a fungal strain kkuf-0955, isolated from decayed cyanobacterial bloom was tested for its capability to inhibit phytoplankton species in batch cultures. the strain was identified as tricho ... | 2014 | 24874888 |
| phylogeny of the clinically relevant species of the emerging fungus trichoderma and their antifungal susceptibilities. | a set of 73 isolates of the emerging fungus trichoderma isolated from human and animal clinical specimens were characterized morphologically and molecularly using a multilocus sequence analysis that included the internal transcribed spacer (its) regions of the nuclear ribosomal dna and fragments of the translation elongation factor 1 alpha (tef1), endochitinase chi18-5 (chi18-5), and actin 1 (act1) genes. the most frequent species was trichoderma longibrachiatum (26%), followed by trichoderma ci ... | 2014 | 24719448 |
| 20-residue and 11-residue peptaibols from the fungus trichoderma longibrachiatum are synergistic in forming na+/k+ -permeable channels and adverse action towards mammalian cells. | certain species of the filamentous fungal genus trichoderma (e.g. trichoderma longibrachiatum and trichoderma citrinoviride) are among the emerging clinical pathogens and also the most common species in the indoor space of mould-damaged buildings. the molecules involved in its pathology are not known. in the present study, we report that 0.5-2.6 wt% of the t. longibrachiatum mycelial biomass consisted of thermostable secondary metabolites mitochondriotoxic to mammalian cells. these were identifi ... | 2012 | 22994321 |
| biochemical and proteomic characterization of a novel extracellular β-glucosidase from trichoderma citrinoviride. | β-glucosidases are of pivotal importance in bioconversion of carbonic biomass into fermentable and other useful metabolites, food industry, biotransformation, glyco-trimming of metabolome, etc. trichoderma citrinoviride when grown on delignified lantana camara produced a β-glucosidase and secreted it out in the medium. the extracellularly secreted β-glucosidase of t. citrinoviride was homogeneity purified and then characterized for its kinetic properties and proteomic characteristics. the 90 kda ... | 2013 | 22438061 |