| dimethylsulfide is an energy source for the heterotrophic marine bacterium sagittula stellata. | dimethylsulfide (dms) is a volatile organosulfur compound, ubiquitous in the oceans, that has been credited with various roles in biogeochemical cycling and in climate control. various oceanic sinks of dms are known - both chemical and biological - although they are poorly understood. in addition to the utilization of dms as a carbon or a sulfur source, some bacteria are known to oxidize it to dimethylsulfoxide (dmso). sagittula stellata is a heterotrophic member of the alphaproteobacteria found ... | 2011 | 21718347 |
| mameliella alba gen. nov., sp. nov., a marine bacterium of the roseobacter clade in the order rhodobacterales. | a gram-negative, non-motile, rod-shaped bacterial strain, jlt354-w(t), that accumulates poly-beta-hydroxybutyrate granules was isolated from the south china sea. phylogenetic analysis based on 16s rrna gene sequences showed that the strain was related to members of the genera antarctobacter, sagittula, oceanicola and loktanella; levels of similarity between strain jlt354-w(t) and members of the above genera were less than 92.0 %. the predominant fatty acid of strain jlt354-w(t) was c(18 : 1)omeg ... | 2010 | 19661508 |
| optimized isolation procedure for obtaining strongly actinide binding exopolymeric substances (eps) from two bacteria (sagittula stellata and pseudomonas fluorescens biovar ii). | different chemical extractants (nacl, edta, hcl and naoh) and physical methods (ultrasonication and heating) were examined by their efficacies of extracting "attached" exopolymeric substances (eps) secreted by marine bacterium sagittula stellata (ss) and terrestrial bacterium pseudomonas fluorescens biovar ii (pf). extraction by 0.5 n hcl for 3 h was best for ss while extraction by 0.05 n nacl for 3-5 h was regarded as optimal for pf. improvements in eps purification included a pre-diafiltration ... | 2009 | 19574036 |
| in situ reverse transcription, an approach to characterize genetic diversity and activities of prokaryotes. | reverse transcription of rna molecules inside intact bacterial cells was carried out by using reverse transcriptase with a single oligonucleotide complementary to specific 16s rrna or mrna sequences. fluorescently labeled nucleotides were incorporated into each transcribed cdna inside cells. this protocol is termed in situ reverse transcription (isrt). in this study, by using species-specific primers targeting unique regions of the 16s rrna sequences, isrt was used successfully to detect and enu ... | 1997 | 16535753 |
| [juvenile fish in a tidal pool, térraba-sierpe forest reserve, puntarenas, costa rica]. | juvenile fish were sampled with a 10 m long net in a tide pool (17,000 m2) on the west margin of boca guarumal, térraba-sierpe forest reserve, puntarenas, costa rica, from october 1992 through january 1994. water temperature and surface salinity were recorded in each visit. the specimens were fixed in 5% formaldehyde and preserved in 70% ethanol. abundance and size data were pooled based on precipitation, a main ecological influence in the reserve. a total of 13,494 individuals from 18 species w ... | 2001 | 15264544 |
| key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine roseobacter lineage. | aromatic compound degradation in six bacteria representing an ecologically important marine taxon of the alpha-proteobacteria was investigated. initial screens suggested that isolates in the roseobacter lineage can degrade aromatic compounds via the beta-ketoadipate pathway, a catabolic route that has been well characterized in soil microbes. six roseobacter isolates were screened for the presence of protocatechuate 3,4-dioxygenase, a key enzyme in the beta-ketoadipate pathway. all six isolates ... | 2000 | 11055908 |
| roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline ekho lake. | eight gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic ekho lake (vestfold hills, east antarctica). the cells contained storage granules and daughter cells could be motile. bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. the strains tolerated a wide range of temperature, ph, concentrations of artificial seawater and nacl, but had an absolute requirement for sodi ... | 1999 | 10028255 |
| antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal ekho lake. | four gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic ekho lake (vestfold hills, east antarctica). the cells contained storage granules and formed rosettes. daughter cells may be motile. growth required sodium ions. nitrate was reduced to nitrite, and dissimilatory reduction of nitrite was possible. dnase and gelatinase were produced. glutamate was metabolized with and without an additional source of combined n ... | 1998 | 9828438 |
| mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. | the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ... | 2008 | 18065535 |
| sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. | a numerically important member of marine enrichment cultures prepared with lignin-rich, pulp mill effluent was isolated. this bacterium was gram negative and rod shaped, did not form spores, and was strictly aerobic. the surfaces of its cells were covered by blebs or vesicles and polysaccharide fibrils. each cell also had a holdfast structure at one pole. the cells formed rosettes and aggregates. during growth in the presence of lignocellulose or cellulose particles, cells attached to the surfac ... | 1997 | 9226910 |