| phenotypic and genotypic identification of yeasts from cheese. | eighty-five years strains isolated from different cheeses of austria, denmark, france, germany, and italy were identified using physiological methods and genotypically using random amplified polymorphic dna polymerase chain reaction (rapd-pcr) analysis. good congruence was found between the phenotypic and genotypic data for 39 of the isolates. however, 26 isolates of geotrichum could only be identified to the species level using the genotypic methods and 7 isolates were correctly identified to t ... | 1999 | 10510714 |
| differentiation of debaryomyces hansenii and candida famata by rrna gene intergenic spacer fingerprinting and reassessment of phylogenetic relationships among d. hansenii, c. famata, d. fabryi, c. flareri (=d. subglobosus) and d. prosopidis: description of d. vietnamensis sp. nov. closely related to d. nepalensis. | the intergenic spacer rdna amplification and alui fingerprinting (igsaf) method detected four distinct groups among 170 debaryomyces hansenii strains: d. hansenii var. hansenii; candida famata var. famata; d. hansenii var. fabryi and c. famata var. flareri. igs sequence comparison of representative strains showed that d. hansenii var. hansenii and c. famata var. famata belonged to one species, whereas d. hansenii var. fabryi and c. famata var. flareri belonged to two different ones. this confirm ... | 2009 | 19385997 |
| delimitation of the species of the debaryomyces hansenii complex by intron sequence analysis. | the delineation of species among strains assigned to debaryomyces hansenii was examined using a gene genealogies-based approach in order to compare spliceosomal intron sequences found in four housekeeping genes (act1, tub2, rpl31 and rpl33). this revealed four distinct groups of strains containing, respectively, d. hansenii var. hansenii cbs 767(t), d. hansenii var. fabryi cbs 789(t), candida famata var. flareri cbs 1796(t) (the anamorph of d. hansenii var. fabryi cbs 789(t)) and debaryomyces ty ... | 2009 | 19406826 |
| polyphasic re-examination of debaryomyces hansenii strains and reinstatement of d. hansenii, d. fabryi and d. subglobosus. | the type species of the genus debaryomyces, debaryomyces hansenii, is a highly heterogeneous species. it has been isolated from a large diversity of natural sources including fruit, air, water, soil, but most frequently from processed food products. the species delineation of this yeast species urgently needs clarification. the heterogeneity in taxonomic characteristics has resulted in the description of varieties linked to d. hansenii. the aim of this study was to re-examine and classify d. han ... | 2008 | 20396575 |
| xylh encodes a xylose/h+ symporter from the highly related yeast species debaryomyces fabryi and debaryomyces hansenii. | the closely related yeasts debaryomyces fabryi and debaryomyces hansenii are excellent xylose consumers. we previously described the activity of a high-affinity xylose/h(+) symport from an industrial strain of d. hansenii subsequently reclassified as d. fabryi. we now report the identification of the gene encoding this permease, ay347871.2. this was retrieved from d. fabryi gdna using a degenerate primer pcr strategy, based on conserved regions from the amino acid sequences of three well-charact ... | 2013 | 23809840 |
| candida guilliermondii and other species of candida misidentified as candida famata: assessment by vitek 2, dna sequencing analysis, and matrix-assisted laser desorption ionization-time of flight mass spectrometry in two global antifungal surveillance programs. | candida famata (teleomorph debaryomyces hansenii) has been described as a medically relevant yeast, and this species has been included in many commercial identification systems that are currently used in clinical laboratories. among 53 strains collected during the sentry and artemis surveillance programs and previously identified as c. famata (includes all submitted strains with this identification) by a variety of commercial methods (vitek, microscan, api, and auxacolor), dna sequencing methods ... | 2013 | 23100350 |
| immune gene expression profile of penaeus monodon in response to marine yeast glucan application and white spot syndrome virus challenge. | immunostimulant potential of eight marine yeast glucans (yg) from candida parapsilosis r20, hortaea werneckii r23, candida spencermartinsiae r28, candida haemulonii r63, candida oceani r89, debaryomyces fabryi r100, debaryomyces nepalensis r305 and meyerozyma guilliermondii r340 were tested against wssv challenge in penaeus monodon post larvae (pl). structural characterization of these marine yeast glucans by proton nuclear magnetic resonance (nmr) indicated structures containing (1-6)-branched ... | 2015 | 25555812 |
| draft genome of debaryomyces fabryi cbs 789t, isolated from a human interdigital mycotic lesion. | the yeast genus debaryomyces comprises species isolated from various natural habitats, man-made environments, and clinical materials. here, the draft genome of d. fabryi cbs 789(t), isolated from a human interdigital mycotic lesion, is presented. | 2016 | 26847909 |
| development of species-specific primers for rapid identification of debaryomyces hansenii. | in this work, we developed a specific pcr assay for debaryomyces hansenii strains that uses a putative homologous pad1 region (729 bp) present in this yeast species as a target. the amplification of this sequence with the d. hansenii specific primer pair (dhpadf/dhpadr) was found to be a rapid, specific and an affordable method enabling identification of d. hansenii from other yeast strains. primers were tested in almost 100 strains, 49 strains from type culture collection belonging to the genus ... | 2015 | 25462930 |
| a simple mathematical model that describes the growth of the area and the number of total and viable cells in yeast colonies. | we propose a model, based on the gompertz equation, to describe the growth of yeasts colonies on agar medium. this model presents several advantages: (i) one equation describes the colony growth, which previously needed two separate ones (linear increase of radius and of the squared radius); (ii) a similar equation can be applied to total and viable cells, colony area or colony radius, because the number of total cells in mature colonies is proportional to their area; and (iii) its parameters es ... | 2014 | 25099389 |
| salt and oxidative stress tolerance in debaryomyces hansenii and debaryomyces fabryi. | we report the characterization of five strains belonging to the halotolerant highly related debaryomyces hansenii/fabryi species. the analysis performed consisted in studying tolerance properties, membrane characteristics, and cation incell amounts. we have specifically investigated (1) tolerance to different chemicals, (2) tolerance to osmotic and salt stress, (3) tolerance and response to oxidative stress, (4) reactive oxygen species (ros) content, (5) relative membrane potential, (6) cell vol ... | 2013 | 23122272 |