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biting behavior of anopheles mosquitoes in costa marques, rondonia, brazil.mosquito collections were made in and near costa marques, rondonia, brazil, to determine anopheline anthropophilic/zoophilic behavior. collections from a non-illuminated, bovine-baited trap and indoor and outdoor human-bait collections were compared. anopheles darlingi and anopheles deaneorum were more anthropophilic than the other anophelines collected. the remainder of the anopheles species were collected much more frequently in bovine-baited traps than in human-bait collections. anopheles dar ...19911815282
colonization and maintenance of anopheles deaneorum in brazil.anopheles deaneorum, an important malaria vector and a member of the anopheles albitarsis complex, was colonized by an artificial mating technique. morphological and behavioral differences between an. deaneorum and an. albitarsis from costa marques, rondonia, brazil, are discussed. the essential methods and colonization techniques are described. immature development and mortality rates were reduced when dried grass was added to larval rearing pans. males frequently mate more than once using the ...19902230782
[variation of anopheles density with deltamethrin-impregnated mosquito nets in an endemic malaria area of the brazilian amazon].in 1992 a survey on the use of deltamethrin-impregnated mosquito nets was conducted in the municipality of costa marques, rondonia. in the intradomicile, impregnated nets decreased the vector density at rates similar to those for non-impregnated nets during low-transmission periods; during high anopheline density periods, they led to a significant reduction in vector density, while in the non-impregnated net group there was an increase in the number of anophelines captured. there was no change i ...199910409781
the anopheles albitarsis complex with the recognition of anopheles oryzalimnetes wilkerson and motoki, n. sp. and anopheles janconnae wilkerson and sallum, n. sp. (diptera: culicidae).the anopheles (nyssorhynchus) albitarsis complex includes six species: an. albitarsis, anopheles oryzalimnetes wilkerson and motoki, n. sp., anopheles marajoara, anopheles deaneorum, anopheles janconnae wilkerson and sallum, n. sp. and an. albitarsis f. except for an. deaneorum, species of the complex are indistinguishable when only using morphology. the problematic distinction among species of the complex has made study of malaria transmission and ecology of an. albitarsis s.l. difficult. conse ...200919876554
analysis of the evolutionary forces shaping mitochondrial genomes of a neotropical malaria vector complex.many vectors of human malaria belong to complexes of morphologically indistinguishable cryptic species. here we report the analysis of the newly sequenced complete mitochondrial dna molecules from six recognized or putative species of one such group, the neotropical anopheles albitarsis complex. the molecular evolution of these genomes had been driven by purifying selection, particularly strongly acting on the rna genes. directional mutation pressure associated with the strand-asynchronous asymm ...201121241811
identification of a fibrinogen-related protein (fbn9) gene in neotropical anopheline mosquitoes.malaria has a devastating impact on worldwide public health in many tropical areas. studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. a member of the fibrinogen-related protein family, fbn9, has been well studied in anopheles gambiae and has been shown to be an important component of the mosquito immune system. however, little is known about this gene in neotropical anopheline speci ...201121288344
mitochondrial genome sequences reveal deep divergences among anopheles punctulatus sibling species in papua new guinea.members of the anopheles punctulatus group (ap group) are the primary vectors of human malaria in papua new guinea. the ap group includes 13 sibling species, most of them morphologically indistinguishable. understanding why only certain species are able to transmit malaria requires a better comprehension of their evolutionary history. in particular, understanding relationships and divergence times among anopheles species may enable assessing how malaria-related traits (e.g. blood feeding behavio ...201323405960
abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary colombian malaria endemic area.in colombia for several years, the urabá-bajo cauca and alto sinú region has registered the highest numbers of malaria cases in the country. malaria vector incrimination and the characterization of entomological parameters will allow for a better understanding of malaria transmission dynamics and the design of effective vector control strategies for this region.201323497535
hybrid sterility in crosses between two brazilian sibling species of the anopheles albitarsis complex.complexes of cryptic species are common in several taxa and this is also the case in the anopheles genus, a group including all known human malaria vectors. the anopheles albitarsis complex comprises at least nine cryptic species, some of which are implicated as vectors of human malaria. several different types of data have been generated for this species complex such as cytogenetics, alloenzymes, morphological and feeding behavioral, hybridization experiments, rapd-pcr and rflp and mitochondria ...201425471342
cryptic species in the anopheles (nyssorhynchus) albitarsis (diptera: culicidae) complex: incongruence between random amplified polymorphic dna-polymerase chain reaction identification and analysis of mitochondrial dna coi gene sequences.random amplified polymorphic dna (rapd) diagnostic bands are one tool used to differentiate cryptic mosquito species in the anopheles albitarsis complex. monophyly of four species (a. albitarsis lynch-arribálzaga, a. albitarsis b, a. deaneorum rosa-freitas, and a. marajoara galvão & damasceno) currently identified with the rapd technique was assessed using sequences of the cytochrome oxidase i (coi) mitochondrial dna (mtdna) gene. maximum parsimony, maximum likelihood, and bayesian analyses supp ...017082822
comparative mt genomics of the tipuloidea (diptera: nematocera: tipulomorpha) and its implications for the phylogeny of the tipulomorpha.a traditionally controversial taxon, the tipulomorpha has been frequently discussed with respect to both its familial composition and relationships with other nematocera. the interpretation of internal relationships within the tipuloidea, which include the tipulidae sensu stricto, cylindrotomidae, pediciidae and limoniidae, is also problematic. we sequenced the first complete mitochondrial (mt) genome of symplecta hybrida (meigen, 1804), which belongs to the subfamily chioneinae of family limoni ...201627341029
an overview of malaria transmission from the perspective of amazon anopheles vectors.in the americas, areas with a high risk of malaria transmission are mainly located in the amazon forest, which extends across nine countries. one keystone step to understanding the plasmodium life cycle in anopheles species from the amazon region is to obtain experimentally infected mosquito vectors. several attempts to colonise anopheles species have been conducted, but with only short-lived success or no success at all. in this review, we review the literature on malaria transmission from the ...201525742262
integrated vector management targeting anopheles darlingi populations decreases malaria incidence in an unstable transmission area, in the rural brazilian amazon.studies on vector behaviour should be conducted in order to evaluate the effectiveness of vector control measures on malaria protection in endemic areas of latin america, where p. vivax predominates. this work aims to investigate the fauna of anopheline mosquitoes and verify the impact of integrated vector management in two colonization projects in the careiro municipality, western brazilian amazon.201223088224
contribution of fish farming ponds to the production of immature anopheles spp. in a malaria-endemic amazonian town.in the past decade fish farming has become an important economic activity in the occidental brazilian amazon, where the number of new fish farms is rapidly increasing. one of the primary concerns with this phenomenon is the contribution of fishponds to the maintenance and increase of the anopheline mosquito population, and the subsequent increase in human malaria burden. this study reports the results of a 2-year anopheline abundance survey in fishponds and natural water bodies in a malaria-ende ...201526573145
the mitochondrial genome of elodia flavipalpis aldrich (diptera: tachinidae) and the evolutionary timescale of tachinid flies.tachinid flies are natural enemies of many lepidopteran and coleopteran pests of forests, crops, and fruit trees. in order to address the lack of genetic data in this economically important group, we sequenced the complete mitochondrial genome of the palaearctic tachinid fly elodia flavipalpis aldrich, 1933. usually found in northern china and japan, this species is one of the primary natural enemies of the leaf-roller moths (tortricidae), which are major pests of various fruit trees. the 14,932 ...201323626734
population dynamics, structure and behavior of anopheles darlingi in a rural settlement in the amazon rainforest of acre, brazil.anopheles darlingi is the major vector of malaria in south america, and its behavior and distribution has epidemiological importance to biomedical research. in brazil, an. darlingi is found in the northern area of the amazon basin, where 99.5% of the disease is reported.201121702964
a new mtdna coi gene lineage closely related to anopheles janconnae of the albitarsis complex in the caribbean region of colombia.an understanding of the taxonomic status and vector distribution of anophelines is crucial in controlling malaria. previous phylogenetic analyses have supported the description of six species of the neotropical malaria vector anopheles (nyssorhynchus) albitarsis s.l. (diptera: culicidae): an. albitarsis, anopheles deaneorum, anopheles marajoara, anopheles oryzalimnetes, anopheles janconnae and an. albitarsis f. to evaluate the taxonomic status of an. albitarsis s.l. mosquitoes collected in vario ...021225199
lineage divergence detected in the malaria vector anopheles marajoara (diptera: culicidae) in amazonian brazil.cryptic species complexes are common among anophelines. previous phylogenetic analysis based on the complete mtdna coi gene sequences detected paraphyly in the neotropical malaria vector anopheles marajoara. the "folmer region" detects a single taxon using a 3% divergence threshold.201020929572
seasonal distribution of malaria vectors (diptera: culicidae) in rural localities of porto velho, rondônia, brazilian amazon.we conducted a survey of the malaria vectors in an area where a power line had been constructed, between the municipalities of porto velho and rio branco, in the states of rondônia and acre, respectively. the present paper relates to the results of the survey of anopheles fauna conducted in the state of rondônia. mosquito field collections were performed in six villages along the federal highway br 364 in the municipality of porto velho, namely porto velho, jaci paraná, mutum paraná, vila abunã, ...026200969
adaptation of a south american malaria vector to laboratory colonization suggests faster-male evolution for mating ability.anopheles (nyssorhynchus) albitarsis (diptera: culicidae) is one of the very few south american mosquito vectors of malaria successfully colonized in the laboratory. these vectors are very hard to breed because they rarely mate in artificial conditions. a few years ago a free-mating laboratory colony of an. albitarsis sensu stricto was established after about 30 generations of artificial-mating. to begin to understand the process of adaptation of these malaria vectors to the laboratory we have c ...200415132759
malaria vectors in south america: current and future scenarios.malaria remains a significant public health issue in south america. future climate change may influence the distribution of the disease, which is dependent on the distribution of those anopheles mosquitoes competent to transmit plasmodium falciparum. herein, predictive niche models of the habitat suitability for p. falciparum, the current primary vector anopheles darlingi and nine other known and/or potential vector species of the neotropical albitarsis complex, were used to document the current ...201526283539
geographic distribution, evolution, and disease importance of species within the neotropical anopheles albitarsis group (diptera, culicidae).the anopheles albitarsis group of mosquitoes comprises eight recognized species and one mitochondrial lineage. our knowledge of malaria vectorial importance and the distribution and evolution of these taxa is incomplete. we constructed ecological niche models (enms) for these taxa and used hypothesized phylogenetic relationships and enms to investigate environmental and ecological divergence associated with speciation events. two major clades were identified, one north (clade 1) and one south (c ...024820570
hybridization evidence supporting separate species status for anopheles albitarsus and anopheles deaneorum (diptera: culicidae) in brazil. 19911895089
comparative susceptibility of anopheline mosquitoes to plasmodium falciparum in rondonia, brazil.five anopheline species, anopheles deaneorum, an. albitarsis, an. triannulatus, an. oswaldoi, and an. mediopunctatus were compared to an. darlingi for susceptibility to infection by p. falciparum in costa marques, rondonia, brazil. laboratory reared f1 an. darlingi and anopheline test species were allowed to feed at the same time on falciparum malaria patients who had gametocytes in their blood, and who had not yet been treated with quinine. mosquitoes were dissected and examined for oocysts on ...19911858963
anopheles deaneorum: a new potential malaria vector in state of santa catarina, brazil (diptera: culicidae). 201323563843
analysis of reproductive isolation between sibling species anopheles albitarsis sensu stricto and anopheles deaneorum, two malaria vectors belonging to the albitarsis complex (diptera: culicidae).complexes of sibling species are common among mosquitoes, and their existence within vector species can have important epidemiological consequences. anopheles albitarsis sensu stricto and anopheles deaneorum rosa-freitas are two putative vectors of malaria parasites belonging to the albitarsis species complex (diptera: culicidae). using an induced mating technique, we studied the reproductive isolation between these two closely related species and their reciprocal hybrids. evidence for hybrid ma ...200415535617
intragenomic rdna its2 variation in the neotropical anopheles (nyssorhynchus) albitarsis complex (diptera: culicidae).we cloned and sequenced the rdna internal transcribed spacer 2 (its2) of 4 species belonging to the neotropical anopheles (nyssorhynchus) albitarsis complex, that is, a. albitarsis; a. albitarsis b; anopheles marajoara, a proven malaria vector; and anopheles deaneorum, a suspected vector. even though the its2 sequences of these species were very similar (< or =1.17% divergence), we found differences suitable for species identification and intragenomic variation of possible consequence in phyloge ...200817158469
composition of anopheline (diptera: culicidae) community and its seasonal variation in three environments of the city of puerto iguazú, misiones, argentina.in order to extend the knowledge of the composition of the anopheline community and the seasonal variation related to anthropogenic modifications in the city of puerto iguazú, adult females were captured between 2009 and 2012. samples were collected in three environments with different degrees of anthropogenic modification: urban, periurban, and wild. alpha diversity was evaluated as the 'true' diversity of the species in each environment. among environments, range-abundance curves were used to ...201729194513
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