Publications

TitleAbstractYear
Filter
PMID
Filter
isolation of tobacco necrosis virus from strawberry leaves in the czech republic.leaves of symptomless fragaria ananassa duch cv. cacanská raná were grafted onto fragaria vesca indicator clones. thirty-five of 72 grafted indicator plants developed leaf mottle symptoms. isometric virus-like particles were observed in purified preparations from symptomatic leaves of f. vesca. the latter were mechanically inoculated to herbaceous host plants. a virus was successfully purified from nicotiana occidentalis 37 b symptomatic plants by differential and sucrose density gradient centri ...199810358734
multiplex rt-pcr detection of four aphid-borne strawberry viruses in fragaria spp. in combination with a plant mrna specific internal control.the principal aphid-borne viruses infecting strawberry (fragaria spp.) strawberry crinkle virus (scv), strawberry mild yellow edge virus (smyev), strawberry mottle virus (smov) and strawberry vein banding virus (svbv) can cause serious crop losses. in this paper, a multiplex reverse transcriptase polymerase chain reaction (rt-pcr) method is described for the simultaneous detection of all four viruses in combination with a plant mrna specific internal control which can be used as an indicator of ...200312880923
[fragaria vesca as a cholagogue]. 196414255531
correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from bulgarian herbs.the water phase antioxidant activity of extracts from 23 bulgarian medicinal plants was studied in relation to their polyphenol content in comparison with mate, black tea, honeybush and rooibos foreign species. antioxidant activity was measured by the abts (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) cation radical decolorization assay, and the total polyphenol content was assayed according to the folin-ciocalteu method. five bulgarian plant extracts exhibited higher antioxidant activ ...200616906640
wild vascular plants gathered for consumption in the polish countryside: a review.this paper is an ethnobotanical review of wild edible plants gathered for consumption from the end of the 18th century to the present day, within the present borders of poland.200717433114
traditional knowledge of wild edible plants used in the northwest of the iberian peninsula (spain and portugal): a comparative study.we compare traditional knowledge and use of wild edible plants in six rural regions of the northwest of the iberian peninsula as follows: campoo, picos de europa, piloña, sanabria and caurel in spain and parque natural de montesinho in portugal.200717555572
[an orientational examination of the effects of extracts from mixtures of herbal drugs on selected renal functions].the paper aimed to determine the effects of mixtures of selected medicinal plants on some physiological renal functions, i.e. excretion of urine and electrolytes and changes in the quantity of prostaglandins e2 (pge2) and kallikrein-kinins in rat blood plasma after water and salt load. the following medicinal plants were selected for the examination: downy birch (betula pubescens ehrh.), everlasting flower (helichrysum arenarium l. moench.), hawthorn (crataegus oxyacantha l.), woodland strawberr ...200717619305
an inbred line of the diploid strawberry fragaria vesca f. semperflorens for genomic and molecular genetic studies in the rosaceae.abstract:200919878589
active package for wild strawberry fruit (fragaria vesca l.).an antimicrobial active package has been developed to improve the safety and quality of wild strawberries, as well as extending their shelf life. the fruits were packed in equilibrium-modified atmosphere packaging (emap), and the effect on botrytis cinerea growth and on the quality parameters of the fruit by the addition of different amounts of 2-nonanone, an antifungal volatile compound naturally present in strawberries, was investigated during storage at 10 and 22 degrees c. the temperature of ...200717316018
molecular interaction between methylobacterium extorquens and seedlings: growth promotion, methanol consumption, and localization of the methanol emission site.four methylobacterium extorquens strains were isolated from strawberry (fragaria x ananassa cv. elsanta) leaves, and one strain, called me4, was tested for its ability to promote the growth of various plant seedlings. seedling weight and shoot length of nicotiana tabacum, lycopersicon esculentum, sinapis alba, and fragaria vesca increased significantly in the presence of the pink-pigmented facultative methylotroph (ppfm), but the germination behaviour of seeds from six other plants was not affec ...200617043084
effect of fruit extract of fragaria vesca l. on experimentally induced inflammatory bowel disease in albino rats.ulcerative colitis and crohn's disease are chronic recurrent inflammatory bowel disease (ibd) of unknown origin. oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in ibd.201121455415
controlled atmosphere storage of wild strawberry fruit (fragaria vesca l.).controlled atmosphere storage technology to extend the shelf life of "reina de los valles" wild strawberry fruit (fragaria vesca l.) was studied. fruits were stored at 3 degrees c for three weeks in different atmosphere compositions: 0.05% co2/21% o2 (air), 3% co2/18% o2, 6% co2/15% o2, 10% co2/11% o2, and 15% co2/6% o2. the effect of gas composition on soluble solids content, titrable acidity, ph, off-flavor, aroma volatiles, and consumer preference was monitored. the result showed that the 10% ...200616390182
high-efficiency transformation of the diploid strawberry (fragaria vesca) for functional genomics.fragaria vesca l., a diploid (2n = 2x = 14) relative of the commercial octoploid strawberry, is an attractive model for functional genomics research in rosaceae. its small genome size, short reproductive cycle, and facile vegetative and seed propagation make f. vesca a promising candidate for forward and reverse genetics experiments. however, the lack of a high-efficiency transformation protocol required for systematic production of thousands of t-dna insertional mutant lines and high-throughput ...200616320068
isolation and diversity analysis of resistance gene analogues (rgas) from cultivated and wild strawberries.degenerate oligonucleotide primers, designed based on conserved regions of nucleotide binding site (nbs) domains from previously cloned plant resistance genes, were used to isolate resistance gene analogues (rgas) from wild and cultivated strawberries. seven distinct families of rgas of the nbs-lrr type were identified from two related wild species, fragaria vesca and f. chiloensis, and six different fragaria x ananassa cultivars. with one exception (gav-3), the deduced amino acid sequences of s ...200415565466
vegetation geography of western part of elmacik mountain, turkey.the study examines the western part of elmacik mountain from the perspective of vegetation geography. research area is within the phytogeographical region of euro-siberian that is among the flora and phytogeographical region of turkey. according to turkey's grid square system, the research area is located in the a3 square. the main elements of forest formation consist of fagus orientalis, abies nordmanniana subspp. bommuelleriana, pinus nigra subsp. pallasiana, pinus sylvestris, quercus spp., pl ...201223424832
gain and loss of fruit flavor compounds produced by wild and cultivated strawberry species.the blends of flavor compounds produced by fruits serve as biological perfumes used to attract living creatures, including humans. they include hundreds of metabolites and vary in their characteristic fruit flavor composition. the molecular mechanisms by which fruit flavor and aroma compounds are gained and lost during evolution and domestication are largely unknown. here, we report on processes that may have been responsible for the evolution of diversity in strawberry (fragaria spp) fruit flav ...200415522848
functional characterization of enzymes forming volatile esters from strawberry and banana.volatile esters are flavor components of the majority of fruits. the last step in their biosynthesis is catalyzed by alcohol acyltransferases (aats), which link alcohols to acyl moieties. full-length cdnas putatively encoding aats were isolated from fruit of wild strawberry (fragaria vesca) and banana (musa sapientum) and compared to the previously isolated saat gene from the cultivated strawberry (fragaria x ananassa). the potential role of these enzymes in fruit flavor formation was assessed. ...200415326278
the defh9-iaam auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry.the defh9-iaam gene fusion which is expressed specifically in placenta/ovules and promotes auxin-synthesis confers parthenocarpic fruit development to eggplant, tomato and tobacco. transgenic defh9-iaam eggplants and tomatoes show increased fruit production due mainly to an improved fruit set. however, the weight of the fruits is also frequently increased.200415113427
characterization and comparison of the cpk gene family in the apple (malus × domestica) and other rosaceae species and its response to alternaria alternata infection.as one of the ca2+ sensors, calcium-dependent protein kinase (cpk) plays vital roles in immune and stress signaling, growth and development, and hormone responses, etc. recently, the whole genome of apple (malus × domestica), pear (pyrus communis), peach (prunus persica), plum (prunus mume) and strawberry (fragaria vesca) in rosaceae family has been fully sequenced. however, little is known about the cpk gene family in these rosaceae species. in this study, 123 cpk genes were identified from fiv ...201627186637
unfoldome variation upon plant-pathogen interactions: strawberry infection by colletotrichum acutatum.intrinsically disordered proteins (idps) are proteins that lack secondary and/or tertiary structure under physiological conditions. these proteins are very abundant in eukaryotic proteomes and play crucial roles in all molecular mechanisms underlying the response to environmental challenges. in plants, different idps involved in stress response have been identified and characterized. nevertheless, a comprehensive evaluation of protein disorder in plant proteomes under abiotic or biotic stresses ...201526245354
high variability and disomic segregation of microsatellites in the octoploid fragaria virginiana mill. (rosaceae).the objectives of the present study were to develop microsatellite markers for the wild strawberry, fragaria virginiana, to evaluate segregation patterns of microsatellite alleles in this octoploid species, and assess genetic variability at microsatellite loci in a wild population. a genomic library was screened for microsatellite repeats and several pcr primers were designed and tested. we also tested the use of heterologous primers and found that f. virginiana primers amplified products in cul ...200312908097
in vitro antiviral activity of a series of wild berry fruit extracts against representatives of picorna-, orthomyxo- and paramyxoviridae.wild berry species are known to exhibit a wide range of pharmacological activities. they have long been traditionally applied for their antiseptic, antimicrobial, cardioprotective and antioxidant properties. the aim of the present study is to reveal the potential for selective antiviral activity of total methanol extracts, as well as that of the anthocyanins and the non-anthocyanins from the following wild berries picked in bulgaria: strawberry (fragaria vesca l.) and raspberry (rubus idaeus l.) ...201424660461
identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected lactobacillus strains.this study aimed to identify lactic acid bacteria (lab) in byproducts of fruit (malpighia glabra l., mangifera indica l., annona muricata l., and fragaria vesca l.) pulp processing. fifty strains of lab were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequence (16s rrna) analysis. species belonging to lactobacillus genus were the predominant lab in all fruit pulp processing byproducts. the average congruency betwe ...201627625647
infectivity of recombinant strawberry vein banding virus dna.infectivity of the cloned dna genome of strawberry vein banding virus (svbv) was demonstrated by particle bombardment of 4-week-old strawberry (fragaria vesca l. var. uc-5) plants with gold particles coated with the putative full-length 7.9 kb viral dna. vein banding symptoms developed on 15 % of inoculated plants 6-7 weeks post-inoculation. an approximate 1.25-mer of the viral dna was cloned into the binary vector pcgn1547. particle bombardment of this construct into strawberry plants gave an i ...200312771404
root cooperation in a clonal plant: connected strawberries segregate roots.the ability to selectively avoid competition with members of the same clone should be highly advantageous but has not been demonstrated in plants. we found that physical connection between plants in a clone of the wild strawberry fragaria chiloensis induced them to segregate their roots, significantly increasing clonal performance. such increase in performance was not found when plants were grown in containers that artificially divided their rooting zones. there was no effect of connection in a ...200312647182
characterization and complete nucleotide sequence of strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses.an isolate of strawberry mottle virus (smov) was transferred from fragaria vesca to nicotiana occidentalis and chenopodium quinoa by mechanical inoculation. electron micrographs of infected tissues showed the presence of isometric particles of approximately 28 nm in diameter. smov-associated tubular structures were also conspicuous, particularly in the plasmodesmata of c. quinoa. dsrna extraction of smov-infected n. occidentalis yielded two bands of 6.3 and 7.8 kbp which were cloned and sequence ...200211752720
[phytoperfusion of the bladder after adenomectomy].for continuous irrigation of the bladder after prostatic adenomectomy herb infusion (urtica dioica l. 12-15 g/l, hypericum perforatum l. 8-12 g/l. marticaria recutita l. 8-10 g/l, folia plantaginis majoris 7-10 g/l, herba millefolii 4-6 g/l, folia betula 3-5 g/l. artemisia vulgaris l. 1-2 g/l, folia fragaria vesca 3-4 g/l, water 11 maximum) was dripped through a thin suprapubic and urethral drainages. such phytoperfusion of the urinary bladder used in 22 patients reduced postoperative blood loss ...19958571476
clustering of foodstuffs in food hypersensitivity. an inquiry study in pollen allergic patients.600 pollen allergic patients were questioned about hypersensitivity symptoms from various foodstuffs. the answers regarding one foodstuff were compared with those of other foodstuffs making 780 tables, from which the spearmen 's correlation coefficients were calculated. most of the combinations of foodstuffs showed statistically significant correlations. the highest value of the correlation coefficient was found for certain combinations: various nuts reciprocally as well as nuts combined with ap ...19846731203
procyanidins from the roots of fragaria vesca: characterization and pharmacological approach. 19883409389
a new potexvirus associated with strawberry mild yellow edge disease.a physical map of cdna clones prepared from dsrna associated with the my-18 source of strawberry mild yellow edge (smye) was constructed and 854 nucleotides adjacent to the 3' poly(a) tail were sequenced. the larger open reading frame product of mr 25714 showed considerable amino acid homology to the coat protein cistrons of six potexviruses and two carlaviruses. a second product of mr 11216 encoded completely within the coat protein cistron, but in a different frame, has similarities to two pot ...19902191082
composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species.sugars, organic acids, and total phenolic content in fruit of 25 wild and cultivated berry species were identified and quantified with high-performance liquid chromatograph. the composition of sugars, organic acids, and total phenolic compounds in various species of vaccinium, rubus, ribes, and fragaria genus was evaluated. additonally, total phenolics of less known berry species of the morus, amelanchier, sorbus, sambucus, rosa, lycium, actinidia, and aronia genus were determined in wild growin ...201222924969
genome-wide snp identification by high-throughput sequencing and selective mapping allows sequence assembly positioning using a framework genetic linkage map.determining the position and order of contigs and scaffolds from a genome assembly within an organism's genome remains a technical challenge in a majority of sequencing projects. in order to exploit contemporary technologies for dna sequencing, we developed a strategy for whole genome single nucleotide polymorphism sequencing allowing the positioning of sequence contigs onto a linkage map using the bin mapping method.201021192788
de novo assembly and characterization of transcriptomes of early-stage fruit from two genotypes of annona squamosa l. with contrast in seed number.annona squamosa l., a popular fruit tree, is the most widely cultivated species of the genus annona. the lack of transcriptomic and genomic information limits the scope of genome investigations in this important shrub. it bears aggregate fruits with numerous seeds. a few rare accessions with very few seeds have been reported for annona. a massive pyrosequencing (roche, 454 gs flx+) of transcriptome from early stages of fruit development (0, 4, 8 and 12 days after pollination) was performed to pr ...201525766098
comparative genetic mapping points to different sex chromosomes in sibling species of wild strawberry (fragaria).separate sexes have evolved repeatedly from hermaphroditic ancestors in flowering plants, and thus select taxa can provide unparalleled insight into the evolutionary dynamics of sex chromosomes that are thought to be shared by plants and animals alike. here we ask whether two octoploid sibling species of wild strawberry--one almost exclusively dioecious (males and females), fragaria chiloensis, and one subdioecious (males, females, and hermaphrodites), f. virginiana--share the same sex-determini ...201020923978
implementing reverse genetics in rosaceae: analysis of t-dna flanking sequences of insertional mutant lines in the diploid strawberry, fragaria vesca.reverse genetics is used for functional genomics research in model plants. to establish a model system for the systematic reverse genetics research in the rosaceae family, we analyzed genomic dna flanking the t-dna insertions in 191 transgenic plants of the diploid strawberry, fragaria vesca. one hundred and seventy-six t-dna flanking sequences were amplified from the right border (rb) and 37 from the left border (lb) by thermal asymmetric interlaced pcr. analysis of the t-dna nick positions rev ...201020444194
larval survival of fuller's rose weevil, naupactus cervinus, on common groundcover species in orchards of new zealand kiwifruit.fuller's rose weevil, naupactus cervinus (boheman) (curculionidae: entiminae), is an important quarantine pest of new zealand kiwifruit exported to asian markets. both adults and larvae are considered to be polyphagous. in this study, the survival of larval n. cervinus was estimated on common groundcover species of kiwifruit (actinidia spp.) in the bay of plenty, the main region in new zealand where kiwifruit is grown. the botanical composition of groundcover in commercial kiwifruit orchards, ch ...200820298112
[evaluation of anti-inflammatory activity of extracts from siberian plants].experimental investigations have shown that water-alcohol extracts from plants containing alkaloids (aconitum baikalense, aconitum septentrionale, delphinium elatum l., conium maculatum) and salicylic acid (filipendula ulmaria, salix viminalis, fragaria vesca, rubus idaeus) inhibited the development of main symptoms of inflammation, viz. exudation, pain, fever, to the same extent as non-steroidal anti-inflammatory agents. the substances studied in this work may be used to develop new efficient p ...200920017405
evaluation of genome sequencing quality in selected plant species using expressed sequence tags.with the completion of genome sequencing projects for more than 30 plant species, large volumes of genome sequences have been produced and stored in online databases. advancements in sequencing technologies have reduced the cost and time of whole genome sequencing enabling more and more plants to be subjected to genome sequencing. despite this, genome sequence qualities of multiple plants have not been evaluated.201323922843
assessment of anti-oxidant activity of plant extracts using microbial test systems.to evaluate the anti-oxidant properties of extracts from 20 medicinal herbs growing in western siberia using microbial test systems and different in vitro methods.200919187149
cardiovascular effects in vitro of aqueous extract of wild strawberry (fragaria vesca, l.) leaves.in contrast to the strawberry fruits, strawberry leaves as a source of bioactive compounds with potentially beneficial biological effects have been largely overlooked. in this study we examined direct, dose-dependent effects of wild strawberry (fragaria vesca, l.) leaves aqueous extract, in two experimental models and animal species, the isolated guinea pig hearts and rat aortic rings. vasodilatory potential of the wild strawberry leaves extract was compared with vasodilatory activity of aqueous ...200919131227
genetic diversity of pto-like serine/threonine kinase disease resistance genes in cultivated and wild strawberries.degenerate oligonucleotide primers, designed based on conserved regions of several serine-threonine kinases (stk) previously cloned in tomato and arabidopsis, were used to isolate stk candidates in wild and cultivated strawberries. seven distinct classes of stks were identified from three related wild species, i.e., fragaria vesca, fragaria chiloensis, and potentilla tucumanensis, and seven different fragaria x ananassa cultivars. alignment of the deduced amino acid sequences and the pto r prote ...200818618068
template mixing: a method of enhancing detection and interpretation of codominant rapd markers.ten codominant rapd markers, ranging in size from about 300 to about 1350 bp, were identified in mapping populations of chickpea (cicer arietinum l.) and diploid strawberry (fragaria vesca l.). a distinguishing feature of all ten markers, and perhaps of codominant rapd markers in general, was the presence in heterozygous individuals of a non-parental, heteroduplex band migrating more slowly than either of the respective parental bands. this non-parental band could also be generated by mixing par ...199524169884
partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (fragaria vesca).in addition to the role of the cell wall as a physical barrier against pathogens, some of its constituents, such as pectin-derived oligogalacturonides (oga), are essential components for elicitation of defence responses. to investigate how modifications of pectin alter defence responses, we expressed the fruit-specific fragaria x ananassa pectin methyl esterase fape1 in the wild strawberry fragaria vesca. pectin from transgenic ripe fruits differed from the wild-type with regard to the degree an ...200818088306
demethylation of oligogalacturonides by fape1 in the fruits of the wild strawberry fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruit.ectopic expression of the strawberry (fragaria×ananassa) gene fape1 encoding pectin methyl esterase produced in the wild species fragaria vesca partially demethylated oligogalacturonides (ogas), which conferred partial resistance of ripe fruits to the fungus botrytis cinerea. analyses of metabolic and transcriptional changes in the receptacle of the transgenic fruits revealed channelling of metabolites to aspartate and aromatic amino acids as well as phenolics, flavanones, and sesquiterpenoids, ...201121273336
are flower-visiting ants mutualists or antagonists? a study in a gynodioecious wild strawberry.ants are common flower visitors, but their effects on plant reproductive fitness have not often been assessed. flower-visiting ants were studied to determine whether they are antagonists or mutualists and whether they could influence floral or breeding system evolution in gynodioecious wild strawberry (fragaria virginiana). ant and flying pollinator (bees/flies) access to plants was manipulated, and visitation, fruit, and seed set were assessed. ants visited flowers of hermaphrodites more often ...200521652471
pseudomonas fluorescens f113 mutant with enhanced competitive colonization ability and improved biocontrol activity against fungal root pathogens.motility is one of the most important traits for efficient rhizosphere colonization by pseudomonas fluorescens f113rif (f113). in this bacterium, motility is a polygenic trait that is repressed by at least three independent pathways, including the gac posttranscriptional system, the wsp chemotaxis-like pathway, and the sadb pathway. here we show that the kinb gene, which encodes a signal transduction protein that together with algb has been implicated in alginate production, participates in swim ...201121685161
hepatoprotective activity of a phytotherapeutic formula on thioacetamide--induced liver fibrosis model.hepatic fibrosis is a widespread alteration in the liver that primarily consists of increased collagen deposition in the tissue. the aim of the present study was to evaluate the protective effects of poly-phytocompound eh-1501 containing small amounts of silymarin but also other potentially effective substances on thioacetamide (tta)-induced liver fibrosis and to elucidate the mechanisms underlying these protective effects in rats. forty rats were randomly divided into four groups. liver fibrosi ...201122069962
characterization and functional analysis of calmodulin and calmodulin-like genes in fragaria vesca.calcium is a universal messenger that is involved in the modulation of diverse developmental and adaptive processes in response to various stimuli. calmodulin (cam) and calmodulin-like (cml) proteins are major calcium sensors in all eukaryotes, and they have been extensively investigated for many years in plants and animals. however, little is known about cams and cmls in woodland strawberry (fragaria vesca). in this study, we performed a genome-wide analysis of the strawberry genome and identif ...201627990153
development of an efficient transformation method by agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (fragaria vesca) using nptii selection.key message : we developed an efficient agrobacterium -mediated transformation method using an ac/ds transposon tagging construct for f. vesca and high throughput paromomycin spray assay to identify its transformants for strawberry functional genomics. genomic resources for rosaceae species are now readily available, including the fragaria vesca genome, est sequences, markers, linkage maps, and physical maps. the rosaceae genomic executive committee has promoted strawberry as a translational gen ...201323160638
shoot regeneration and agrobacterium-mediated transformation of fragaria vesca l.an efficient and reliable method for shoot regeneration from leaf disks of fragaria vesca l. has been developed. this protocol has been successfully employed to obtain transformed plants using agrobacterium tumefaciens as gene vector. murashige and skoog basal medium supplemented with benzyladenine (4 mg/l) and indole-3-butyric acid (0.25 mg/l) induced the maximum percentage of shoot regeneration (98%) and the highest number of shoot colonies per explant (4.6) after 8 weeks of culture. isolated ...199624178534
genome-wide identification and comparative expression analysis of nbs-lrr-encoding genes upon colletotrichum gloeosporioides infection in two ecotypes of fragaria vesca.anthracnose caused by colletotrichum spp. is one of the most destructive diseases of cultivated strawberry (fragaria×ananassa duchesne) worldwide. the correlation between nbs-lrr genes, the largest class of known resistance genes, and strawberry anthracnose resistance has been elusive. blast search in ncbi identified 94 fvnbss in the diploid genome of strawberry fragaria vesca, with 67 of the tir-nbs-lrr type. at least 36 fvnbss were expressed, with 25% being non-coding genes. two f. vesca ecoty ...201323806759
functional characterization and substrate promiscuity of ugt71 glycosyltransferases from strawberry (fragaria × ananassa).glycosylation determines the complexity and diversity of plant natural products. to characterize fruit ripening-related udp-dependent glycosyltransferases (ugts) functionally in strawberry, we mined the publicly available fragaria vesca genome sequence and found 199 putative ugt genes. candidate ugts whose expression levels were strongly up-regulated during fruit ripening were cloned from f.×ananassa and six were successfully expressed in escherichia coli and biochemically characterized. ugt75t1 ...201526454881
analysis of block of cell proliferation 1 (bop1) activity in strawberry and arabidopsis.block of cell proliferation (bop) proteins are conserved among eukaryotes, and studies in mammals and yeast have described their role in ribosome biogenesis and cell cycle regulation. a bop1 orthologue was identified in plants, and loss-of-function analyses in tobacco cells confirmed similar activities. this report characterizes a role for bop1 activity in planta. two transgenic plant species were used: the diploid strawberry (fragaria vesca) and arabidopsis thaliana. fvbop1 silencing showed cha ...201626940494
infectious cdna clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the disease.a full-length in vivo infectious cdna clone of strawberry mild yellow edge-associated potexvirus (smyeav) was constructed and used to inoculate fragaria vesca 'alpine' seedlings and rubus rosifollus. both host plants could be infected using particle bombardment or agroinoculation, but not by mechanical inoculation. a method that used potted strawberry plants for particle bombardment resulted in high survival and infection rates. the plants developed systemic infection and virus particles were de ...19979292025
quantitative trait variation is revealed in a novel hypomethylated population of woodland strawberry (fragaria vesca).phenotypic variation is determined by a combination of genotype, environment and their interactions. the realization that allelic diversity can be both genetic and epigenetic allows the environmental component to be further separated. partitioning phenotypic variation observed among inbred lines with an altered epigenome can allow the epigenetic component controlling quantitative traits to be estimated. to assess the contribution of epialleles on phenotypic variation and determine the fidelity w ...201627809774
complete nucleotide sequence of strawberry vein banding virus chinese isolate and infectivity of its full-length dna clone.strawberry vein banding virus (svbv) is a double-stranded dna plant virus, which has been found in north america, australia, brazil, japan, europe and several provinces of china. infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins. it is one of the most economically important diseases in asiatic, european and north american strawberry-growing areas.201627716385
transcriptome analysis of woodland strawberry (fragaria vesca) response to the infection by strawberry vein banding virus (svbv).woodland strawberry (fragaria vesca) infected with strawberry vein banding virus (svbv) exhibits chlorotic symptoms along the leaf veins. however, little is known about the molecular mechanism of strawberry disease caused by svbv.201627411713
quarantine strawberry vein banding virus firstly detected in slovakia and serbia.strawberry vein banding virus (svbv) was detected by polymerase chain reaction (pcr) and dot-blot hybridisation in samples of cultivated strawberry plants originating from central slovakia and in samples of wild strawberry plants from south-eastern serbia in federal republic of yugoslavia (fry). this is the first finding of svbv in these countries as well as of svbv in wild strawberry plants in europe.19989770076
genomic identification, phylogeny, and expression analysis of mlo genes involved in susceptibility to powdery mildew in fragaria vesca.the mlo (powdery mildew locus o) gene family is important in resistance to powdery mildew (pm). in this study, all of the members of the mlo family were identified and analyzed in the strawberry (fragaria vesca) genome. the strawberry contains at least 20 members of the mlo family, and the protein sequence contained between 171 and 1485 amino acids, with 0-34 introns. chromosomal localization showed that the mlos were unevenly distributed on each of the chromosomes, except for chromosome 4. the ...201627525914
reprogramming of strawberry (fragaria vesca) root transcriptome in response to phytophthora cactorum.crown rot (phytophthora cactorum) causes significant economic losses in strawberry production. the best control strategy would be to use resistant cultivars, but polygenically inherited resistance makes the breeding of the garden strawberry (fragaria × ananassa) challenging. the diploid wild strawberry fragaria vesca hawaii 4 genotype was shown previously to have resistance against crown rot. to explore the resistance mechanisms, we inoculated the roots of hawaii 4 with p. cactorum in a novel in ...201627518577
the arabidopsis npr1 gene confers broad-spectrum disease resistance in strawberry.although strawberry is an economically important fruit crop worldwide, production of strawberry is limited by its susceptibility to a wide range of pathogens and the lack of major commercial cultivars with high levels of resistance to multiple pathogens. the objective of this study is to ectopically express the arabidopsis thaliana npr1 gene (atnpr1) in the diploid strawberry fragaria vesca l. and to test transgenic plants for disease resistance. atnpr1 is a key positive regulator of the long-la ...201525812515
genome-wide identification and characterization of r2r3myb family in rosaceae.transcription factors r2r3myb family have been associated with the control of secondary metabolites, development of structures, cold tolerance and response to biotic and abiotic stress, among others. in recent years, genomes of rosaceae botanical family are available. although this information has been used to study the karyotype evolution of these species from an ancestral genome, there are no studies that treat the evolution and diversity of gene families present in these species or in the bot ...201627408811
comparative analysis of predicted plastid-targeted proteomes of sequenced higher plant genomes.plastids are actively involved in numerous plant processes critical to growth, development and adaptation. they play a primary role in photosynthesis, pigment and monoterpene synthesis, gravity sensing, starch and fatty acid synthesis, as well as oil, and protein storage. we applied two complementary methods to analyze the recently published apple genome (malus × domestica) to identify putative plastid-targeted proteins, the first using targetp and the second using a custom workflow utilizing a ...201425393533
the strawberry gene fagast affects plant growth through inhibition of cell elongation.the strawberry (fragaria x ananassa) fagast gene encodes a small protein with 12 cysteine residues conserved in the c-terminal region similar to a group of proteins identified in other species with diverse assigned functions such as cell division, elongation, or elongation arrest. this gene is expressed in the fruit receptacle, with two peaks during ripening at the white and the red-ripe stages, both coincident with an arrest in the growth pattern. expression is also high in the roots but confin ...200616804055
synteny analysis in rosids with a walnut physical map reveals slow genome evolution in long-lived woody perennials.mutations often accompany dna replication. since there may be fewer cell cycles per year in the germlines of long-lived than short-lived angiosperms, the genomes of long-lived angiosperms may be diverging more slowly than those of short-lived angiosperms. here we test this hypothesis.201526383694
homomorphic zw chromosomes in a wild strawberry show distinctive recombination heterogeneity but a small sex-determining region.recombination in ancient, heteromorphic sex chromosomes is typically suppressed at the sex-determining region (sdr) and proportionally elevated in the pseudoautosomal region (par). however, little is known about recombination dynamics of young, homomorphic plant sex chromosomes. we examine male and female function in crosses and unrelated samples of the dioecious octoploid strawberry fragaria chiloensis in order to map the small and recently evolved sdr controlling both traits and to examine rec ...201627102236
evolutionary origins and dynamics of octoploid strawberry subgenomes revealed by dense targeted capture linkage maps.whole-genome duplications are radical evolutionary events that have driven speciation and adaptation in many taxa. higher-order polyploids have complex histories often including interspecific hybridization and dynamic genomic changes. this chromosomal reshuffling is poorly understood for most polyploid species, despite their evolutionary and agricultural importance, due to the challenge of distinguishing homologous sequences from each other. here, we use dense linkage maps generated with targete ...201425477420
comparative analysis of fruit aroma patterns in the domesticated wild strawberries "profumata di tortona" (f. moschata) and "regina delle valli" (f. vesca).strawberry is one of the most valued fruit worldwide. modern cultivated varieties (fragaria × ananassa) exhibit large fruits, with intense color and prolonged shell life. yet, these valuable traits were attained at the cost of the intensity and the variety of the aroma of the berry, two characteristics highly appreciated by consumers. wild species display smaller fruits and reduced yield compared with cultivated varieties but they accumulate broader and augmented blends of volatile compounds. be ...201525717332
early metabolic and transcriptional variations in fruit of natural white-fruited fragaria vesca genotypes.strawberry fruits (fragaria vesca) are valued for their sweet fruity flavor, juicy texture, and characteristic red color caused by anthocyanin pigments. to gain a deeper insight into the regulation of anthocyanin biosynthesis, we performed comparative metabolite profiling and transcriptome analyses of one red-fruited and two natural white-fruited strawberry varieties in two tissues and three ripening stages. developing fruit of the three genotypes showed a distinctive pattern of polyphenol accum ...201728327625
endophytic fungal diversity of fragaria vesca, a crop wild relative of strawberry, along environmental gradients within a small geographical area.fungal endophytes are highly diverse ubiquitous asymptomatic microorganisms, some of which appear to be symbiotic. depending on abiotic conditions and genotype of the plant, the diversity of endophytes may confer fitness benefits to plant communities.201728168102
partial preferential chromosome pairing is genotype dependent in tetraploid rose.it has long been recognised that polyploid species do not always neatly fall into the categories of auto- or allopolyploid, leading to the term 'segmental allopolyploid' to describe everything in between. the meiotic behaviour of such intermediate species is not fully understood, nor is there consensus as to how to model their inheritance patterns. in this study we used a tetraploid cut rose (rosa hybrida) population, genotyped using the 68k wagrhsnp array, to construct an ultra-high-density lin ...201728142191
identification and transcript analysis of the tcp transcription factors in the diploid woodland strawberry fragaria vesca.plant-specific teosinte branched 1, cycloidea, and proliferating cell factors (tcp) transcription factors play versatile functions in multiple processes of plant growth and development. however, no systematic study has been performed in strawberry. in this study, 19 fvtcp genes were identified in the diploid woodland strawberry (fragaria vesca) accession heilongjiang-3. phylogenetic analysis suggested that the fvtcp genes were classified into two main classes, with the second class further divid ...201628066489
global identification of alternative splicing via comparative analysis of smrt- and illumina-based rna-seq in strawberry.alternative splicing (as) is a key post-transcriptional regulatory mechanism, yet little information is known about its roles in fruit crops. here, as was globally analyzed in the wild strawberry fragaria vesca genome with rna-seq data derived from different stages of fruit development. the as landscape was characterized and compared between the single-molecule, real-time (smrt) and illumina rna-seq platform. while smrt has a lower sequencing depth, it identifies more genes undergoing as (57.67% ...201727997733
pathway analysis and omics data visualization using pathway genome databases: fragariacyc, a case study.the species-specific plant pathway genome databases (pgdbs) based on the biocyc platform provide a conceptual model of the cellular metabolic network of an organism. such frameworks allow analysis of the genome-scale expression data to understand changes in the overall metabolisms of an organism (or organs, tissues, and cells) in response to various extrinsic (e.g. developmental and differentiation) and/or extrinsic signals (e.g. pathogens and abiotic stresses) from the surrounding environment. ...201727987175
stable epigenetic variants selected from an induced hypomethylated fragaria vesca population.epigenetic inheritance was transmitted through selection over five generations of extreme early, but not late flowering time phenotypic lines in fragaria vesca. epigenetic variation was initially artificially induced using the dna demethylation reagent 5-azacytidine (5-azac). it is the first report to explore epigenetic variant selection and phenotypic trait inheritance in strawberry. transmission frequency of these traits was determined across generations. the early flowering (ef4) and late sto ...201627965682
high-density snp-based genetic maps for the parents of an outcrossed and a selfed tetraploid garden rose cross, inferred from admixed progeny using the 68k rose snp array.dense genetic maps create a base for qtl analysis of important traits and future implementation of marker-assisted breeding. in tetraploid rose, the existing linkage maps include <300 markers to cover 28 linkage groups (4 homologous sets of 7 chromosomes). here we used the 68k wagrhsnp axiom single-nucleotide polymorphism (snp) array for rose, in combination with snp dosage calling at the tetraploid level, to genotype offspring from the garden rose cultivar 'red new dawn'. the offspring proved t ...201627818777
the effect of fragaria vesca extract on smear layer removal: a scanning electron microscopic evaluation.successful endodontic treatment depends on elimination of the microorganisms through chemomechanical debridement. the aim of this in vitro study was to evaluate the effectiveness of fragaria vesca (wild strawberry) extract (fve) on the removal of smear layer (sl).201526526069
polyphenolic glycoconjugates from medical plants of rosaceae/asteraceae family protect human lymphocytes against γ-radiation-induced damage.radioprotective effects of the water-soluble polyphenolic glycoconjugates, isolated from flowers of sanguisorba officinalis l.(so) and erigeron canadensis l.(ec), and from leaves of fragaria vesca l. (fv) and rubus plicatus whe. et n. e. (rp), against γ-radiation-induced toxicity in human peripheral blood lymphocytes were investigated. cell treatment with glycoconjugates (1, 5 and 25μg/ml) prior exposure to 10/15gy radiation resulted in concentration-dependent reduction of dna damage including o ...201727769934
wild fragaria vesca l. fruits: a rich source of bioactive phytochemicals.wild fragaria vesca l. fruits were studied regarding nutritional and phytochemical compounds, and also antioxidant, antibacterial and biofilm formation inhibition activities. the fruits are good sources of carbohydrates (e.g., sucrose), soluble dietary fiber and polyunsaturated fatty acids, mainly linoleic and linolenic acids, as well as other components such as citric and succinic acids, and vitamins b9 and e (mainly γ-tocopherol). significant amounts of soluble sugars, citric acid and some amo ...201627775146
hplc-msn identification and quantification of flavonol glycosides in 28 wild and cultivated berry species.berries and red fruits are rich dietary sources of polyphenols with reported health benefits. more than 50 different flavonols (glycosides of quercetin, myricetin, kaempferol, isorhamnetin, syringetin and laricitrin) have been detected and quantified with hplc-ms(n) in fruits of blueberry, bilberry, cranberry, lingonberry, eastern shadbush, japanese wineberry, black mulberry, chokeberry, red, black and white currants, jostaberry, red and white gooseberry, hardy kiwifruit, goji berry, rowan, dog ...201222980782
plant genotype affects total antioxidant capacity and phenolic contents in fruit.we wanted to determine the total antioxidant capacity (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid equivalent antioxidant assay; teac) of different species and cultivars (strawberry and apple) and to characterize the teac among fruits of the same varieties (peach and apricot) but grafted on different rootstocks.200515723750
identification, isolation, and expression analysis of heat shock transcription factors in the diploid woodland strawberry fragaria vesca.heat shock transcription factors (hsfs) are known to play dominant roles in plant responses to heat, as well as other abiotic or biotic stress stimuli. while the strawberry is an economically important fruit plant, little is known about the hsf family in the strawberry. to explore the functions of strawberry hsfs in abiotic and biotic stress responses, this study identified 17 hsf genes (fvhsfs) in a wild diploid woodland strawberry (fragaria vesca, 2n = 2x = 14) and isolated 14 of these genes. ...201526442049
expression of resistance gene analogs in woodland strawberry (fragaria vesca) during infection with phytophthora cactorum.important losses in strawberry production are often caused by the oomycete phytophthora cactorum, the causal agent of crown rot. however, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. to begin to rectify this situation, a pcr-based approach (nbs profiling) was used to isolate strawberry resistance gene analogs (rgas) with altered expression in response to p. cactorum during a time course (2, 4, 6, 24, 48, 96 and 192 h pos ...201627447867
genome-scale dna variant analysis and functional validation of a snp underlying yellow fruit color in wild strawberry.fragaria vesca is a species of diploid strawberry being developed as a model for the octoploid garden strawberry. this work sequenced and compared the genomes of three f. vesca accessions: 'hawaii 4', 'rügen', and 'yellow wonder'. genome-scale analyses of shared and distinct snps among these three accessions have revealed that 'rügen' and 'yellow wonder' are more similar to each other than they are to 'hawaii 4'. though all three accessions are inbred seven generations, each accession still poss ...201627377763
high resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of rosa.rosaceae is a family containing many economically important fruit and ornamental species. although fluorescence in situ hybridization (fish)-based physical mapping of plant genomes is a valuable tool for map-based cloning, comparative genomics and evolutionary studies, no studies using high resolution physical mapping have been performed in this family. previously we proved that physical mapping of single-copy genes as small as 1.1 kb is possible on mitotic metaphase chromosomes of rosa wichuran ...201526134672
detection of strawberry necrotic shock virus using conventional and taqman(®) quantitative rt-pcr.graft-indexing of an advanced selection from the university of florida strawberry breeding program produced virus-like symptoms on fragaria vesca. however; rt-pcr testing of the material did not detect the presence of any of 16 strawberry virus species or members of virus groups for which strawberries are routinely indexed. large scale sequencing of the material revealed the presence of an isolate of strawberry necrotic shock virus. the nucleotide sequence of this isolate from florida shows a si ...201627283883
the genome of black raspberry (rubus occidentalis).black raspberry (rubus occidentalis) is an important specialty fruit crop in the us pacific northwest that can hybridize with the globally commercialized red raspberry (r. idaeus). here we report a 243 mb draft genome of black raspberry that will serve as a useful reference for the rosaceae and rubus fruit crops (raspberry, blackberry, and their hybrids). the black raspberry genome is largely collinear to the diploid woodland strawberry (fragaria vesca) with a conserved karyotype and few notable ...201627228578
development and preliminary evaluation of a 90 k axiom® snp array for the allo-octoploid cultivated strawberry fragaria × ananassa.a high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry fragaria × ananassa. short-read sequences from one diploid and 19 octoploid accessions were aligned to the diploid fragaria vesca 'hawaii 4' reference genome to identify single nucleotide polymorphisms (snps) and indels for incorporation into a 90 k affymetrix® axiom® array. we report the development and preliminary evaluation of this array.201525886969
genome-wide identification and comparative analysis of the cation proton antiporters family in pear and four other rosaceae species.the monovalent cation proton antiporters (cpas) play essential roles in plant nutrition, development, and signal transduction by regulating ion and ph homeostasis of the cell. the cpas of plants include the na(+)/h(+) exchanger, k(+) efflux antiporter, and cation/h(+) exchanger families. however, currently, little is known about the cpa genes in rosaceae species. in this study, 220 cpa genes were identified from five rosaceae species (pyrus bretschneideri, malus domestica, prunus persica, fragar ...201627193473
genome-wide analysis of the expression of wrky family genes in different developmental stages of wild strawberry (fragaria vesca) fruit.wrky proteins play important regulatory roles in plant developmental processes such as senescence, trichome initiation and embryo morphogenesis. in strawberry, only fawrky1 (fragaria × ananassa) has been characterized, leaving numerous wrky genes to be identified and their function characterized. the publication of the draft genome sequence of the strawberry genome allowed us to conduct a genome-wide search for wrky proteins in fragaria vesca, and to compare the identified proteins with their ho ...201627138272
species-specific duplications driving the recent expansion of nbs-lrr genes in five rosaceae species.disease resistance (r) genes from different rosaceae species have been identified by map-based cloning for resistance breeding. however, there are few reports describing the pattern of r-gene evolution in rosaceae species because several rosaceae genome sequences have only recently become available.201525759136
the wrky transcription factors in the diploid woodland strawberry fragaria vesca: identification and expression analysis under biotic and abiotic stresses.wrky proteins comprise a large family of transcription factors that play important roles in response to biotic and abiotic stresses and in plant growth and development. to date, little is known about the wrky gene family in strawberry. in this study, we identified 62 wrky genes (fvwrkys) in the wild diploid woodland strawberry (fragaria vesca, 2n = 2x = 14) accession heilongjiang-3. according to the phylogenetic analysis and structural features, these identified strawberry fvwrky genes were clas ...201627105420
identification and characterization of histone lysine methylation modifiers in fragaria vesca.the diploid woodland strawberry (fragaria vesca) is an important model for fruit crops because of several unique characteristics including the small genome size, an ethylene-independent fruit ripening process, and fruit flesh derived from receptacle tissues rather than the ovary wall which is more typical of fruiting plants. histone methylation is an important factor in gene regulation in higher plants but little is known about its roles in fruit development. we have identified 45 set methyltran ...201627049067
fragariacyc: a metabolic pathway database for woodland strawberry fragaria vesca.fragariacyc is a strawberry-specific cellular metabolic network based on the annotated genome sequence of fragaria vesca l. ssp. vesca, accession hawaii 4. it was built on the pathway-tools platform using metacyc as the reference. the experimental evidences from published literature were used for supporting/editing existing entities and for the addition of new pathways, enzymes, reactions, compounds, and small molecules in the database. to date, fragariacyc comprises 66 super-pathways, 488 uniqu ...201626973684
characterization of dna methyltransferase and demethylase genes in fragaria vesca.dna methylation is an epigenetic modification essential for gene regulations in plants, but understanding on how it is involved in fruit development, especially in non-climacteric fleshy fruit, is limited. the diploid woodland strawberry (fragaria vesca) is an important model for non-climacteric fruit crops. in this study, we identified dna methyltransferase genes and demethylase genes in fragaria vesca and other angiosperm species. in accordance with previous studies, our phylogenetic analyses ...201626956009
mutation in terminal flower1 reverses the photoperiodic requirement for flowering in the wild strawberry fragaria vesca.photoperiodic flowering has been extensively studied in the annual short-day and long-day plants rice (oryza sativa) and arabidopsis (arabidopsis thaliana), whereas less is known about the control of flowering in perennials. in the perennial wild strawberry, fragaria vesca (rosaceae), short-day and perpetual flowering long-day accessions occur. genetic analyses showed that differences in their flowering responses are caused by a single gene, seasonal flowering locus, which may encode the f. vesc ...201222566495
genetic variation in plant cyp51s confers resistance against voriconazole, a novel inhibitor of brassinosteroid-dependent sterol biosynthesis.brassinosteroids (brs) are plant steroid hormones with structural similarity to mammalian sex steroids and ecdysteroids from insects. the brs are synthesized from sterols and are essential regulators of cell division, cell elongation and cell differentiation. in this work we show that voriconazole, an antifungal therapeutic drug used in human and veterinary medicine, severely impairs plant growth by inhibiting sterol-14α-demethylation and thereby interfering with br production. the plant growth ...201323335967
the fragaria vesca homolog of suppressor of overexpression of constans1 represses flowering and promotes vegetative growth.in the annual long-day plant arabidopsis thaliana, suppressor of overexpression of constans1 (soc1) integrates endogenous and environmental signals to promote flowering. we analyzed the function and regulation of the soc1 homolog (fragaria vesca [fv] soc1) in the perennial short-day plant woodland strawberry (fragaria vesca). we found that fv soc1 overexpression represses flower initiation under inductive short days, whereas its silencing causes continuous flowering in both short days and nonind ...201324038650
genome-wide identification and comparative analysis of the heat shock transcription factor family in chinese white pear (pyrus bretschneideri) and five other rosaceae species.heat shock transcription factors (hsfs), which act as important transcriptional regulatory proteins in eukaryotes, play a central role in controlling the expression of heat-responsive genes. at present, the genomes of chinese white pear ('dangshansuli') and five other rosaceae fruit crops have been fully sequenced. however, information about the hsfs gene family in these rosaceae species is limited, and the evolutionary history of the hsfs gene family also remains unresolved.201525604453
effects of high co₂ levels on fermentation, peroxidation, and cellular water stress in fragaria vesca stored at low temperature in conditions of unlimited o₂.to better understand the tolerance of strawberries (fragaria vesca l.) to high co2 in storage atmospheres, fermentation and cellular damage were investigated. fruits were stored for 3 and 6 days at 0 °c in the presence of different co2 levels (0, 20, or 40%) with 20% o2. changes in pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) gene expression and in fermentative metabolites, as well as in bound water and malondialdehyde (mda) concentrations, were analyzed. in strawberries stored w ...201525568930
Displaying items 1 - 100 of 263