an effect of 16s rrna intercistronic variability on coevolutionary analysis in symbiotic bacteria: molecular phylogeny of arsenophonus triatominarum.the genes of ribosomal rna are the most popular and frequently used markers for bacterial phylogeny and reconstruction of insect-symbiont coevolution. in primary symbionts, such as buchnera and wigglesworthia, genome economization leads to the establishment of a single copy of these sequences. in phylogenetic studies, they provide sufficient information and yield phylogenetic trees congruent with host evolution. in contrast, other symbiotic lineages (e.g., the genus arsenophonus) carry a higher ...200818485654
in vitro culture and phylogenetic analysis of "candidatus arsenophonus triatominarum," an intracellular bacterium from the triatomine bug, triatoma intracellular symbiotic bacterium was isolated from the hemolymph of triatoma infestans and cultured in an aedes albopictus cell line. 16s ribosomal dna sequence analysis revealed that the bacterium was a member of the gamma-3 subgroup of the class proteobacteria, having 96.2% sequence identity with the most closely related bacterium, arsenophonus nasoniae, the causative agent of the son-killer trait in the parasitoid wasp nasonia vitripennis. these bacteria share morphological features and a ...19979336921
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