| re-speciation of the original reference strains of serovars in the citrobacter freundii (bethesda-ballerup group) antigenic scheme of west and edwards. | the antigenic scheme for the bethesda-ballerup group of bacteria established by west and edwards in 1954 has continued to be applied as a serotyping scheme for citrobacter freundii. in 1993, however, the classification of the citrobacter was drastically revised and the species c. freundii redefined by brenner et al. accordingly, to judge the propriety to continuously use a single antigenic scheme for the c. freundii complex, the 90 reference strains listed in the antigenic scheme for c. freundii ... | 1996 | 9013489 |
| structure of the o-specific polysaccharide of citrobacter braakii o7a,3b,1c. | the following structure of the o-specific polysaccharide of citrobacter braakii o7a,3b,1c was established using sugar and methylation analyses and nmr spectroscopy, including 2d cosy, tocsy, noesy, and 1h, 13c heteronuclear single-quantum coherence (hsqc) experiments: (struture: see text). the main d-mannan chain of the polysaccharide studied has the same structure as the o-specific polysaccharide of escherichia coli o9, klebsiella pneumoniae o3, and hafnia alvei pcm 1223. | 2001 | 11454340 |
| cytochrome p450(cin) (cyp176a), isolation, expression, and characterization. | cytochromes p450 are members of a superfamily of hemoproteins involved in the oxidative metabolism of various physiologic and xenobiotic compounds in eukaryotes and prokaryotes. studies on bacterial p450s, particularly those involved in monoterpene oxidation, have provided an integral contribution to our understanding of these proteins, away from the problems encountered with eukaryotic forms. we report here a novel cytochrome p450 (p450(cin), cyp176a1) purified from a strain of citrobacter braa ... | 2002 | 12016226 |
| low-virulence citrobacter species encode resistance to multiple antimicrobials. | citrobacter spp. are gram-negative commensal bacteria that infrequently cause serious nosocomial infections in compromised hosts. they are often resistant to cephalosporins due to overexpression of their chromosomal beta-lactamase. during a recent study of multidrug-resistant enterobacteriaceae (mdre) in solid-organ transplant patients, we found that almost half of patients colonized with mdre carried one or more cefpodoxime-resistant citrobacter freundii, citrobacter braakii, or citrobacter ama ... | 2002 | 12384364 |
| cloning and sequencing of the beta-lactamase gene and surrounding dna sequences of citrobacter braakii, citrobacter murliniae, citrobacter werkmanii, escherichia fergusonii and enterobacter cancerogenus. | to further identify the origins of plasmid-mediated cephalosporinases that are currently spreading worldwide, the chromosomal beta-lactamase genes of citrobacter braakii, citrobacter murliniae, citrobacter werkmanii reference strains and of escherichia fergusonii and enterobacter cancerogenus clinical isolates were cloned and expressed into escherichia coli and sequenced. these beta-lactamases had all a single pi value >8 and conferred a typical ampc-type resistance pattern in e. coli recombinan ... | 2002 | 12393205 |
| environmental isolates of citrobacter braakii that agglutinate with escherichia coli o157 antiserum but do not possess the genes responsible for the biosynthesis of o157 somatic antigen. | while searching for escherichia coli o157 in the aquatic environment of calcutta using an immunodetection procedure, we fortuitously detected five strains of citrobacter braakii, which cross-reacted with the commercially available o157 polyvalent antiserum. the five c. braakii isolates gave positive results when a sensitive dot-elisa was performed with e. coli o157 monoclonal antibody. further, the o157 monoclonal antibody recognized the bands of proteinase k treated whole cells of lipopolysacch ... | 2003 | 12729185 |
| isolation and characterization of a phytase with improved properties from citrobacter braakii. | citrobacter braakii yh-15 produced an intracellular phytase which was purified 12800 fold to homogeneity with the specific activity of 3457 units mg(-1), which is 1.9 times higher than e. coli phytase previously recorded as having the highest specific activity. its molecular weight was 47 kda by sds-page gel. enzyme activity was optimal at ph 4 and at 50 degrees c. the km value for sodium phytate was 0.46 mm with a vmax 6027 u mg(-1). the phytase was resistant to proteases such as trypsin, pepsi ... | 2003 | 14514072 |
| molecular cloning of the phytase gene from citrobacter braakii and its expression in saccharomyces cerevisiae. | the gene, appa, encoding phytase was cloned from a size-selected genomic library of citrobacter braakii yh-15 by southern hybridization using a degenerate probe based on the n-terminal amino acid sequence of the phytase. the deduced amino acid sequence of appa contained the n-terminal rhgxrxp motif and the c-terminal hd motif, which are common in histidine acid phosphatases. it also had significant homology (60% identity) with phytase from escherichia coli, while the physical mapping analysis of ... | 2006 | 16369872 |
| characterization of arylsulfatase formed by derepressed synthesis in citrobacter braakii. | arylsulfatase activity was detected in a bacterial strain, citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. the production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. the purified enzyme hydrolyzed 4-nitrophenyl sulfate, 4-nitrocatechol sulfate, and 3-indoxyl sulfate, and was classified as type i arylsulfatase. | 2006 | 16794337 |
| characterization of the enterobacteriaceae isolated from an artisanal italian ewe's cheese (pecorino abruzzese). | to evaluate some physiological characteristics of the enterobacteriaceae isolated from pecorino cheese. | 2006 | 16882142 |
| bactericidal activity of normal bovine serum (nbs) directed against some enterobacteriaceae with sialic acid-containing lipopolysaccharides (lps) as a component of cell wall. | the sensitivity of bacteria to the bactericidal activity of serum depends on the structure and organization of the bacterial outer membrane. sialic acid has been found in the o-specific region of bacterial lipopolysaccharide (lps) and it plays an essential role in protecting gram-negative bacteria against the bactericidal activity of human and animal serum. the susceptibility of gram-negative bacilli with sialic acid-containing lps to the bactericidal action of normal bovine serum (nbs) was dete ... | 2006 | 17338268 |
| glycophorin a is recognized by an antibody population of the rabbit polyclonal antibodies produced against citrobacter braakii o37. | molecular mimicry was found in the case of citrobacter braakii o37, which shares epitopes with human erythrocytes. it is believed that erythrocyte-membrane proteins band 3 and glycophorin a (gpa) have common epitopes. band 3 was recognized by the anti-c. braakii o37 lipopolysaccharide antibodies (lps-abs) purified on lps-affinity columns. this study aimed to investigate the role of gpa in this molecular mimicry. | 2007 | 17446956 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| isolation of faecal coliform bacteria from the american alligator (alligator mississippiensis). | to determine whether american alligators (alligator mississippiensis) are an unrecognized poikilothermic source of faecal coliform and/or potential pathogenic bacteria in south carolina's coastal waters. | 2010 | 19735329 |
| production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages. | histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ... | 2010 | 20673614 |
| effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants. | rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ... | 2010 | 21124052 |
| structures of the o-polysaccharides of salmonella enterica o59 and escherichia coli o15. | the o-polysaccharide of salmonella enterica o59 was studied using sugar analysis and 2d (1)h and (13)c nmr spectroscopy, and the following structure of the tetrasaccharide repeating unit was established: →2)-β-d-galp-(1→3)-α-d-glcpnac-(1→4)-α-l-rhap-(1→3)-β-d-glcpnac-(1→ accordingly, the o-antigen gene cluster of s. enterica o59 includes all genes necessary for the synthesis of this o-polysaccharide. earlier, another structure has been reported for the o-polysaccharide of salmonella arizonae (s. ... | 2010 | 21167479 |
| toxicological studies on a novel phytase expressed from synthetic genes in aspergillus oryzae. | phytases are widely used as feed additives for monogastric animals, which cannot easily utilise the phosphorus bound in phytate (myo-inositol hexakisphosphate). the current study presents a safety evaluation of a 6-phytase produced by an aspergillus oryzae strain expressing two synthetic genes, both mimicking a phytase gene from a citrobacter braakii strain. oral administration of the phytase preparation to rats at a dose level of 0.86 g total organic solids/kg body weight/day for 13 weeks did n ... | 2011 | 21672596 |
| fecal colonization of enterobacteriaceae carrying plasmid-mediated quinolone resistance determinants in korea. | the aims of the current study were to investigate the prevalence and molecular characteristics of plasmid-mediated quinolone resistance (pmqr) genes from colonizing fecal organisms and to compare the incidence and subtype of these genes according to bacterial species and hospital at five tertiary-care hospitals in korea. a total of 500 nonduplicated clinical isolates of enterobacteriaceae were obtained from fecal specimens at five tertiary-care hospitals between march and may 2008. the pmqr gene ... | 2011 | 21830908 |
| comparative effects of three phytases on the phosphorus and calcium use in the weaned piglet. | the addition of phytase to swine diets has generally increased p digestibility and consequently reduced fecal excretion of p. the comparative effects on p and ca digestibility of dietary inclusion of 5 different phytases were evaluated in the weaned piglet. ronozyme hiphos is a microbial 6-phytase produced by synthetic genes, mimicking a gene from citrobacter braakii, and was compared to the escherichia coli-derived phytases phyzyme and optiphos. in total, 112 weaned piglets (28 d old) were allo ... | 2012 | 23365294 |
| rapid identification of carbapenemase genes in gram-negative bacteria with an oligonucleotide microarray-based assay. | rapid molecular identification of carbapenemase genes in gram-negative bacteria is crucial for infection control and prevention, surveillance and for epidemiological purposes. furthermore, it may have a significant impact upon determining the appropriate initial treatment and greatly benefit for critically ill patients. a novel oligonucleotide microarray-based assay was developed to simultaneously detect genes encoding clinically important carbapenemases as well as selected extended (esbl) and n ... | 2014 | 25068267 |
| palladium nanoparticles produced by fermentatively cultivated bacteria as catalyst for diatrizoate removal with biogenic hydrogen. | a new biological inspired method to produce nanopalladium is the precipitation of pd on a bacterium, i.e., bio-pd. this bio-pd can be applied as catalyst in dehalogenation reactions. however, large amounts of hydrogen are required as electron donor in these reactions resulting in considerable costs. this study demonstrates how bacteria, cultivated under fermentative conditions, can be used to reductively precipitate bio-pd catalysts and generate the electron donor hydrogen. in this way, one coul ... | 2011 | 21590286 |
| cloning and expression of phytase appa gene from shigella sp. cd2 in pichia pastoris and comparison of properties with recombinant enzyme expressed in e. coli. | the phytase gene appas was isolated from shigella sp. cd2 genomic library. the 3.8 kb dna fragment contained 1299 bp open reading frame encoding 432 amino acid protein (appas) with 22 amino acid signal peptide at n-terminal and three sites of n-glycosylation. appas contained the active site rhgxrxp and hdtn sequence motifs, which are conserved among histidine acid phosphatases. it showed maximum identity with phytase appa of escherichia coli and citrobacter braakii. the appas was expressed in pi ... | 2016 | 26808559 |
| cloning, expression and purification of cindoxin, an unusual fmn-containing cytochrome p450 redox partner. | cytochromes p450 (p450s) belong to a superfamily of haemoproteins that catalyse a remarkable variety of oxidative transformations. p450 catalysis generally requires that cognate redox proteins transfer electrons, derived ultimately from nad(p)h, to the p450 for oxygen activation. p450(cin) (cyp176a1) is a bacterial p450 that is postulated to allow citrobacter braakii to live on cineole as its sole carbon source by initiating cineole biodegradation. here we report the cloning, expression, purific ... | 2010 | 20419722 |
| fever and prodromal infections in anti-glomerular basement membrane disease. | anti-glomerular basement membrane (gbm) disease is an autoimmune disorder with rapidly progressive glomerulonephritis and alveolar hemorrhage. fever symptom and prodromal infections have been reported in many cases, but still not been elucidated. | 2017 | 28261931 |
| the species accuracy of the most probable number (mpn) european union reference method for enumeration of escherichia coli in marine bivalves. | continuous european union programmes with specified methods for enumeration of escherichia coli in bivalves for human consumption are currently running. the objective of this research was to examine the species accuracy of the five times three tube most probable number (mpn) eu reference method used for detection of e. coli in marine bivalves. among 549 samples of bivalves harvested from norwegian localities during 2014 and 2015, a total number of 200 bacterial isolates were prepared from random ... | 2016 | 27746195 |
| microbial diversity and dynamics during the production of may bryndza cheese. | diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ... | 2014 | 24291178 |
| aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland. | we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ... | 2015 | 25380369 |
| cineole biodegradation: molecular cloning, expression and characterisation of (1r)-6beta-hydroxycineole dehydrogenase from citrobacter braakii. | the first steps in the biodegradation of 1,8-cineole involve the introduction of an alcohol and its subsequent oxidation to a ketone. in citrobacter braakii, cytochrome p450(cin) has previously been demonstrated to perform the first oxidation to produce (1r)-6beta-hydroxycineole. in this study, we have cloned cind from c. braakii and expressed the gene product, which displays significant homology to a number of short-chain alcohol dehydrogenases. it was demonstrated that the gene product of cind ... | 2010 | 20089292 |
| purification, characterization, and gene cloning of glucose-1-phosphatase from citrobacter braakii. | citrobacter braakii produced an intracellular acid glucose phosphatase (agpc) which was purified 986 fold to homogeneity with the specific activity of 286 units/mg. agpc hydrolyzed a wide variety of phosphorylated compounds with high activity for glucose-1-phosphate and glucose-6-phosphate. the optimum ph and temperature for the enzyme activity was ph 5.0 and 45 degrees c, respectively. the km value for glucose-1-phosphate was 5.12 mm with a vmax 27.8 u mg(-1). its molecular weight was 46 kda by ... | 2009 | 19940380 |
| comparison of antimicrobial efficacy of a fixed dose combination of ceftazidime + sulbactam with ceftazidime and sulbactam alone against five bacteria. | the efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (mic) and time-kill curve analysis in pseudomonas aeruginosa, staphylococcus aureus, acinetobacter baumannii, mycobacterium smegmatis, and citrobacter braakii; mic was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/l in fdc of ceftazidime + sulbactam, respectively, which is lower than ceftazidime and sulbactam individually. time-kill curve analysis demonstrated maximum killing of bacteria after 4 ... | 2009 | 19937210 |
| a new method for gene synthesis and its high-level expression. | an optimized citrobacter braakii phytase gene, appa-c, was chemically synthesized by oligonucleotides synthesis and over-lap pcr method. the appa-c gene encoding 423 amino acids was cloned into expression vector ppic9 and transformed into methylotropic yeast pichia pastoris. from about 2000 transformants, 400 transformants exhibiting phytase activity were obtained. one transformant showing the strongest phytase activity was selected for detailed analyses in 5 l bioreactor. under control of the h ... | 2009 | 19733600 |
| physicochemical treatments of anionic surfactants wastewater: effect on aerobic biodegradability. | the effect of different physicochemical treatments on the aerobic biodegradability of an industrial wastewater resulting from a cosmetic industry has been investigated. this industrial wastewater contains 11423 and 3148mgl(-1) of chemical oxygen demand (cod) and anionic surfactants, respectively. the concentration of cod and anionic surfactants were followed throughout the diverse physicochemical treatments and biodegradation experiments. different pretreatments of this industrial wastewater usi ... | 2009 | 18799262 |
| the critical role of substrate-protein hydrogen bonding in the control of regioselective hydroxylation in p450cin. | cytochrome p450cin (cyp176a1) is a bacterial p450 isolated from citrobacter braakii that catalyzes the hydroxylation of cineole to (s)-6beta-hydroxycineole. this initiates the biodegradation of cineole, enabling c. braakii to live on cineole as its sole source of carbon and energy. p450cin lacks the almost universally conserved threonine residue believed to be involved in dioxygen activation and instead contains an asparagine at this position (asn-242). to investigate the role of asn-242 in p450 ... | 2008 | 18270198 |
| antibodies against citrobacter braakii o37 cells recognize the n-glycan of the band 3 glycoprotein of human erythrocyte membrane. | the phenomenon of molecular mimicry was found previously for citrobacter braakii o37, which shared epitopes with human and horse erythrocytes. the aim of this study was to elucidate the basis of the serological cross-reactivity between anti-c. braakii o37 serum and human erythrocytes. the experiments involved analyzing the epitope on the human erythrocyte membrane, that could be recognized by affinity-purified antibodies. the results indicated a specific glycoprotein fraction in immunoblotting, ... | 2008 | 18266742 |
| [overexpression of citrobacter braakii phytase with high specific activity in pichia pastoris]. | utilization of the phytase with high specific activity is an effective way to improve the fermentation potency of phytase in recombinant host and decrease the production cost. up to now, the phytase appa from citrobacter braakii exhibits the highest specific activity in the all phytases recorded previously. the gene appa encoding phytase was modified according to the bias in codon choice of the high expression gene in pichia pastoris without changing the amino acid sequence and artificially synt ... | 2006 | 17302159 |
| a novel phytase with preferable characteristics from yersinia intermedia. | a yersinia intermedia strain producing phytase was isolated from glacier soil. the phytase gene, appa, was isolated by degenerate pcr and tail-pcr. the full-length fragment contained 2354bp with a 1326-bp open reading frame encoding 441 amino acids. appa contained the active site rhgxrxp and hd sequence motifs that are typical of histidine acid phosphatases. to our knowledge, this is the first report of the detection of phytase activity and cloning of the relevant gene from y. intermedia. the ge ... | 2006 | 17034758 |
| removal of selenate in river and drainage waters by citrobacter braakii enhanced with zero-valent iron. | a cost-effective remediation method is needed to remove selenium (se) from se-contaminated water. in this study, a selenate [se(vi)]-reducing bacterium, citrobacter braakii, that is capable of using molasses as a carbon source to reduce se(vi) from natural river and drainage waters was isolated. during an 8-day experiment, 87-97% of the added se(vi) in new river water and white river water, california, was reduced to elemental se [se(0)] or transformed to organic se. in highly saline drainage wa ... | 2006 | 16390192 |
| citrobacter braakii, an unusual organism as cause of acute peritonitis in pd patients. | | 2005 | 16022099 |
| isolation and characterisation of bacterial strains containing enantioselective dmso reductase activity: application to the kinetic resolution of racemic sulfoxides. | the kinetic resolution of racemic sulfoxides by dimethyl sulfoxide (dmso) reductases was investigated with a range of microorganisms. three bacterial isolates (provisionally identified as citrobacter braakii, klebsiella sp. and serratia sp.) expressing dmso reductase activity were isolated from environmental samples by anaerobic enrichment with dmso as terminal electron acceptor. the organisms reduced a diverse range of racemic sulfoxides to yield either residual enantiomer depending upon the st ... | 2004 | 15322772 |
| stereoselective reductase-catalysed deoxygenation of sulfoxides in aerobic and anaerobic bacteria. | direct and indirect evidence, of unexpected stereoselective reductase-catalysed deoxygenations of sulfoxides, was found. the deoxygenations proceeded simultaneously, with the expected dioxygenase-catalysed asymmetric sulfoxidation of sulfides, during some biotransformations with the aerobic bacterium pseudomonas putida uv4. stereoselective reductase-catalysed asymmetric deoxygenation of racemic alkylaryl, dialkyl and phenolic sulfoxides was observed, without evidence of the reverse sulfoxidation ... | 2004 | 14770234 |
| a practical synthesis of alpha-d-manp-(1-->3)-alpha-d-manp-(1-->2)-[alpha-d-glcp-(1-->3)]-alpha-d-manp-(1-->2)-alpha-d-manp-(1-->2)-alpha-d-manp, an o-specific heterohexasaccharide fragment of citrobacter braakii o7a, 3b, 1c. | an o-specific heterohexasaccharide fragment of citrobacter braakii o7a, 3b, 1c, alpha-d-manp-(1-->3)-alpha-d-manp-(1-->2)-[alpha-d-glcp-(1-->3)]-alpha-d-manp-(1-->2)-alpha-d-manp-(1-->2)-alpha-d-manp was synthesized as its methyl glycoside. acetylation of allyl 4,6-o-benzylidene-alpha-d-mannopyranoside, followed by debenzylidenization and benzoylation gave allyl 2,3-di-o-acetyl-4,6-di-o-benzoyl-alpha-d-mannopyranoside (3), and subsequent deacetylation of 3 with ch(3)cocl-meoh gave the monosaccha ... | 2003 | 14553977 |
| sepsis in a renal transplant recipient due to citrobacter braakii. | cellulitis is usually caused by organisms such as beta-hemolytic streptococci and staphylococcus aureus. citrobacter are gram-negative bacilli that can cause opportunistic infections in immunocompromised hosts. they are rarely implicated in skin or soft tissue infections. the genus citrobacter has been respeciated according to genetic relatedness. citrobacter braakii refers to the genomospecies 6 of the citrobacter freundii complex. there are no detailed studies of infections caused by the newly ... | 2003 | 14515921 |
| structural and serological studies on a new 4-deoxy-d-arabino-hexose-containing o-specific polysaccharide from the lipopolysaccharide of citrobacter braakii pcm 1531 (serogroup o6). | the o-specific polysaccharide of citrobacter braakii pcm 1531 (serogroup o6) was isolated by mild acid hydrolysis of the lipopolysaccharide (lps) and found to contain d-fucose, l-rhamnose, 4-deoxy-d-arabino-hexose and o-acetyl groups in molar ratios 2 : 1 : 1 : 1. on the basis of methylation analysis and 1h and 13c nmr spectroscopy data, the structure of the branched tetrasaccharide repeating unit of the o-specific polysaccharide was established. using various serological assays, it was demonstr ... | 2003 | 12823543 |
| cloning and sequencing of the histidine decarboxylase genes of gram-negative, histamine-producing bacteria and their application in detection and identification of these organisms in fish. | the use of molecular tools for early and rapid detection of gram-negative histamine-producing bacteria is important for preventing the accumulation of histamine in fish products. to date, no molecular detection or identification system for gram-negative histamine-producing bacteria has been developed. a molecular method that allows the rapid detection of gram-negative histamine producers by pcr and simultaneous differentiation by single-strand conformation polymorphism (sscp) analysis using the ... | 2003 | 12732523 |
| in vitro and in vivo antimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens. | the aim of this study was to examine the effect of propolis extracts on the microbial colonization of chicken gastrointestinal tract in vivo. the propolis was administered to both feed mixtures in various amounts except of the control group. the addition of 150 mg propolis to 1 kg of feed was included in the first experimental group, the addition of 450 mg.kg(-1) in the second experimental group, the addition of 600 mg.kg(-1) the third experimental group and 800 mg kg(-1) in the fourth one. the ... | 2012 | 22702827 |
| [identification of citrobacter species and their occurrence in raw products and foods]. | fifty-nine strains of bacteria were tested in study that were isolated from raw materials and foods (raw milk, farm milking parlors, sausage meat, raw potatoes, cheese, frozen oven-ready foods, confectionery products, cold dishes), and they were included in the genus citrobacter using a commercial diagnostic kit enterotest 16 (lachema a.s., brno), numeric identification program tnw (czech collection of microorganisms, faculty of science of masaryk university at brno) and identification key (o'ha ... | 1997 | 9182396 |
| classification of citrobacteria by dna hybridization: designation of citrobacter farmeri sp. nov., citrobacter youngae sp. nov., citrobacter braakii sp. nov., citrobacter werkmanii sp. nov., citrobacter sedlakii sp. nov., and three unnamed citrobacter genomospecies. | dna relatedness studies (hydroxyapatite method) were done on 112 strains of citrobacteria. by using the recommended definition of a genomospecies 11 genomospecies were identified in the genus citrobacter. these genomospecies were separable by their biochemical profiles. citrobacter koseri (citrobacter diversus) and citrobacter amalonaticus proved to be homogeneous species, as previously described. c. amalonaticus biogroup 1, as described by farmer et al. (j. clin. microbiol. 21:46-76, 1985), was ... | 1993 | 8240948 |
| occurrence and characteristics of extended spectrum beta-lactamases-producing enterobacteriaceae from foods of animal origin. | presence of extended spectrum beta-lactamases (esbl) in bacteria is a growing health concern of global significance. the local, regional, national, and international epidemiological studies for extended spectrum beta-lactamases-producing enterobacteriaceae and their encoding genes in foods are still incomplete. the objective of this study was to determine the occurrence of extended spectrum beta-lactamases-producing enterobacteriaceae and the characteristics of their encoding genes from a total ... | 2016 | 26991276 |
| the effects of competition from non-pathogenic foodborne bacteria during the selective enrichment of listeria monocytogenes using buffered listeria enrichment broth. | the growth of listeria monocytogenes during the pathogen specific enrichment of food samples can be limited by the presence of additional microorganisms that are resistant to the selective conditions being applied. if growth is severely limited and minimum post-enrichment threshold levels are not met then the presence of l. monocytogenes may go undetected. several food products were screened for non-pathogenic commensal or spoilage microorganisms that are capable of growth under the conditions c ... | 2014 | 25084660 |
| complete genome sequences of citrobacter braakii strains gta-cb01 and gta-cb04, isolated from ground beef. | citrobacter braakii is a gram-negative bacterium belonging to the enterobacteriaceae family. here, we report 5.2- and 5.0-mb genome assemblies for c. braakii strains gta-cb01 and gta-cb04, respectively. | 2015 | 25573940 |
| characterization of qnrb-like genes in citrobacter species of the american type culture collection. | among five american type culture collection (atcc) citrobacter strains, qnrb60 in citrobacter freundii atcc 6879, an isolate from the preantibiotic era, and qnrb61 in citrobacter braakii atcc 51113(t), a type strain belonging to the c. freundii complex, were identified. meanwhile, a truncated qnrb-like pseudogene was identified in c. freundii atcc 8090(t) and atcc 43864. no qnrb-like sequence was found in citrobacter koseri atcc 27028(t). these findings underscore the close relationship between ... | 2013 | 23529729 |
| mechanism of protein kinetic stabilization by engineered disulfide crosslinks. | the impact of disulfide bonds on protein stability goes beyond simple equilibrium thermodynamics effects associated with the conformational entropy of the unfolded state. indeed, disulfide crosslinks may play a role in the prevention of dysfunctional association and strongly affect the rates of irreversible enzyme inactivation, highly relevant in biotechnological applications. while these kinetic-stability effects remain poorly understood, by analogy with proposed mechanisms for processes of pro ... | 2013 | 23936134 |
| performance of microbial phytases for gastric inositol phosphate degradation. | microbial phytases catalyze dephosphorylation of phytic acid, thereby potentially releasing chelated iron and improving human iron absorption from cereal-based diets. for this catalysis to take place in vivo, the phytase must be robust to low ph and proteolysis in the gastric ventricle. this study compares the robustness of five different microbial phytases, evaluating thermal stability, activity retention, and extent of dephosphorylation of phytic acid in a simulated low-ph/pepsin gastric envir ... | 2015 | 25562369 |
| multiwavelength resonance raman characterization of the effect of growth phase and culture medium on bacteria. | we examine the use of multiwavelength ultraviolet (uv) resonance-raman signatures to identify the effects of growth phase and growth medium on gram-positive and gram-negative bacteria. escherichia coli (e. coli), citrobacter koseri (c. koseri), citrobacter braakii (c. braakii), and bacillus cereus (b. cereus) were grown to logarithmic and stationary phases in nutrient broth and brain heart infusion broth. resonance raman spectra of bacteria were obtained at multiple wavelengths between 220 and 2 ... | 2015 | 26163518 |
| selection of dna aptamers for capture and detection of salmonella typhimurium using a whole-cell selex approach in conjunction with cell sorting. | alternative ligands such as nucleic acid aptamers can be used for pathogen capture and detection and offer advantages over antibodies, including reduced cost, ease of production and modification, and improved stability. dna aptamers demonstrating binding specificity to salmonella enterica serovar typhimurium were identified by whole-cell-systematic evolution of ligands by exponential enrichment (selex) beginning with a combinatorial library of biotin-labeled single stranded dna molecules. aptame ... | 2013 | 23494620 |
| citrobacter braakii bacteremia-induced septic shock after colonoscopy preparation with polyethylene glycol in a critically ill patient: a case report. | polyethylene glycol (peg) is widely used for bowel cleaning in preparation for colonoscopy because of its safety. septic shock after peg preparation is an extremely rare complication. herein, we describe a case of septic shock that occurred immediately after colonoscopy preparation with peg. | 2017 | 28376885 |
| citrobacter braakii carrying plasmid-borne mcr-1 colistin resistance gene from ready-to-eat food from a market in the chaco region of bolivia. | | 2017 | 28333311 |
| contribution of selective conditions to microbial competition in four listeria selective enrichment formulations. | microbial competition during selective enrichment negatively affects listeria monocytogenes populations and may hinder the subsequent detection or recovery of this organism. competition assays among 10 selected strains of listeria and citrobacter braakii were performed in buffered listeria enrichment broth, 3-(n-morpholino)propanesulfonic acid-buffered listeria enrichment broth, university of vermont medium-modified listeria enrichment broth, and fraser broth. the individual contributions of eac ... | 2016 | 28221922 |
| cyp101j2, cyp101j3, and cyp101j4, 1,8-cineole-hydroxylating cytochrome p450 monooxygenases from sphingobium yanoikuyae strain b2. | we report the isolation and characterization of three new cytochrome p450 monooxygenases: cyp101j2, cyp101j3, and cyp101j4. these p450s were derived from sphingobium yanoikuyae b2, a strain that was isolated from activated sludge based on its ability to fully mineralize 1,8-cineole. genome sequencing of this strain in combination with purification of native 1,8-cineole-binding proteins enabled identification of 1,8-cineole-binding p450s. the p450 enzymes were cloned, heterologously expressed (n- ... | 2016 | 27590809 |
| citrobacter braakii bacteremia: case report and review of the literature. | bacteremia is usually caused by gram-positive organisms such as staphylococcus aureus or β hemolytic streptococci. while there is a wealth of information that exits for these types of bacteremia, less is available to address bacteremia that is caused by less common bacteria such as citrobacter. we report an unusual case of citrobacter braakii bacteremia in a patient with multiple comorbidities that presented to the hospital with cellulitis and pleural effusion. blood cultures grew citrobacter br ... | 2016 | 27658860 |
| bacteremia due to citrobacter braakii: a case report and literature review. | among the citrobacter genus, the most commonly isolated bacteria from human specimens are citrobacter freundii and citrobacter koseri, and previous cases of infection due to citrobacter braakii have been rarely reported. we present a case of bacteremia due to c. braakii in a 38-year-old woman with cervical cancer. she was admitted to our hospital with complaints of a fever, chills, and nausea. blood culture results showed gram-negative bacilli identified as c. braakii via matrix-assisted laser d ... | 2016 | 27553069 |
| periungual abscess caused by citrobacter braakii in a patient with chronic paronychia. | | 2016 | 27489449 |
| efficient hydroxylation of 1,8-cineole with monoterpenoid-resistant recombinant pseudomonas putida gs1. | in this work, monoterpenoid hydroxylation with pseudomonas putida gs1 and kt2440 were investigated as host strains, and the cytochrome p450 monooxygenase cyp176a1 (p450cin) and its native redox partner cindoxin (cinc) from citrobacter braakii were introduced in p. putida to catalyze the stereoselective hydroxylation of 1,8-cineole to (1r)-6β-hydroxy-1,8-cineole. growth experiments in the presence of 1,8-cineole confirmed pseudomonads' superior resilience compared to e. coli. whole-cell p. putida ... | 2016 | 27263007 |
| a single-day point-prevalence study of faecal carriers in long-term care hospitals in madrid (spain) depicts a complex clonal and polyclonal dissemination of carbapenemase-producing enterobacteriaceae. | the objective of this study was to describe the prevalence and microbiological characteristics of carbapenemase-producing enterobacteriaceae (cpe) colonizing patients in long-term care hospitals (ltchs) in madrid, spain. | 2016 | 26518051 |
| identification and inhibition of histamine-forming bacteria in blue scad (decapterus maruadsi) and chub mackerel (scomber japonicus). | in this study, we investigated the differences in histamine accumulation between blue scad and chub mackerel and methods of inhibiting histamine-forming bacteria and controlling histamine accumulation in fish. the free histidine contents in blue scad and chub mackerel were 1.45 and 2.75 mg/g, respectively. the histamine-forming bacteria isolated from them were identified as citrobacter freundii, citrobacter braakii, and enterobacter aerogenes using 16s rdna sequence analysis, the vitek 2 compact ... | 2015 | 25710155 |
| characterization of beta-lactamases in faecal enterobacteriaceae recovered from healthy humans in spain: focusing on ampc polymorphisms. | the intestinal tract is a huge reservoir of enterobacteriaceae, some of which are opportunist pathogens. several genera of these bacteria harbour intrinsic antibiotic resistance genes, such as ampc genes in species of citrobacter, enterobacter or escherichia genera. in this work, beta-lactamases and other resistance mechanisms have been characterized in enterobacteriaceae isolates recovered from healthy human faecal samples, focusing on the ampc beta-lactamase genes. fifty human faecal samples w ... | 2015 | 25501887 |
| the interplay of dietary nutrient specification and varying calcium to total phosphorus ratio on efficacy of a bacterial phytase: 1. growth performance and tibia mineralization. | a 14-d experiment was conducted to study the effects of 2 dietary variables on efficacy of a 6-phytase from citrobacter braakii on broiler growth performance and tibia mineralization. diets were formulated with or without nutrient matrix values for phytase as negative or positive control (nc or pc, respectively) and with 2 ca:total p (tp; 2:1 or 2.5:1). the diets were supplemented with 0, 1,000, or 2,000 phytase units (fyt)/kg, thus producing a 2 × 2 × 3 factorial arrangement. birds and feed wer ... | 2014 | 25332135 |
| selective oxidation of glycerol to 1,3-dihydroxyacetone by covalently immobilized glycerol dehydrogenases with higher stability and lower product inhibition. | glycerol dehydrogenase (glydh) catalyzes the regioselective oxidation of glycerol to yield 1,3-dihydroxyacetone (dha); an important building block in chemical industry. three recombinant glydhs from geobacillus stearothermophilus, from citrobacter braakii and from cellulomonas sp. were stabilized by covalent immobilization. the highest activity recoveries (40-50%) of the insoluble preparations were obtained by immobilizing these enzymes in presence of polyethylene glycol (peg). noteworthy, these ... | 2014 | 25164336 |
| in vitro and in vivo degradation of myo-inositol hexakisphosphate by a phytase from citrobacter braakii. | phytases (ec 3.1.3) are widely used in animal feed to increase the availability of phosphorus and decrease the anti nutritive effect of myo-inositol hexakisphosphate (insp₆). the aim of this work was to investigate the stereospecific degradation of insp₆ in vitro and in vivo by a phytase from citrobacter braakii (c. braakii), and to study gastric survival of the phytase as well as the site of action in the gastrointestinal tract. the in vitro results showed that the c. braakii phytase belongs to ... | 2012 | 23098167 |
| occurrence of plasmid- and chromosome-carried mcr-1 in waterborne enterobacteriaceae in china. | the aim of this study was to investigate the prevalence of the polymyxin resistance gene mcr-1 in enterobacteriaceae from environmental water sources in hangzhou, china. colistin-resistant bacteria were isolated from environmental water samples using an enrichment broth culture method, were screened for mcr-1, and then were analyzed for the location and transferability of mcr-1 isolates positive for mcr-1 were further examined to determine their susceptibility profiles and were screened for the ... | 2017 | 28559252 |
| characterization of oxa-48-like-producing enterobacteriaceae isolated from river water in algeria. | the spread of carbapenemase-producing enterobacteriaceae (cpe) is a significant problem for healthcare worldwide. the prevalence of carbapenem-resistant enterobacteriaceae (cpe) in water environments in algeria are unknown. the aim of this study was to screen for the presence of cpe isolates in the soummam river in bejaia, algeria. isolates of enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. the isolate ... | 2017 | 28486169 |