| taxonomic dissection of the genus micrococcus: kocuria gen. nov., nesterenkonia gen. nov., kytococcus gen. nov., dermacoccus gen. nov., and micrococcus cohn 1872 gen. emend. | the results of a phylogenetic and chemotaxonomic analysis of the genus micrococcus indicated that it is significantly heterogeneous. except for micrococcus lylae, no species groups phylogenetically with the type species of the genus, micrococcus luteus. the other members of the genus form three separate phylogenetic lines which on the basis of chemotaxonomic properties can be assigned to four genera. these genera are the genus kocuria gen. nov. for micrococcus roseus, micrococcus varians, and mi ... | 1995 | 7547287 |
| polyphasic taxonomy of nesterenkonia halobia. | a phenotypic study has been carried out on six moderately halophilic gram-positive nonmotile cocci isolated from ponds of a saltern located in huelva, spain. these strains were examined for 150 morphological, physiological, biochemical, and nutritional traits and showed phenotypic characteristics similar to those of nesterenkonia halobia (formerly micrococcus halobius). the guanine-plus-cytosine (g + c) content of their dna ranged between 70 and 72 mol%, values quite similar to those described f ... | 1997 | 9336934 |
| moderately halophilic gram-positive bacterial diversity in hypersaline environments. | moderately halophilic bacteria are microorganisms that grow optimally in media containing 3%-15% (w/v) salt. they are represented by a heterogeneous group of microorganisms included in many different genera. gram-negative moderately halophilic bacteria have been studied in more detail, but studies on gram-positive species are more scarce. recent studies carried out by our research group on gram-positive moderate halophiles have permitted clarifying their taxonomic and phylogenetic position and d ... | 1998 | 9783177 |
| precipitation of carbonates by nesterenkonia halobia in liquid media. | we investigated the precipitation of carbonates by nesterenkonia halobia in a liquid medium at different concentrations of salts and incubation times. n. halobia only produced crystals at salt concentrations of 2.5%, 7.5% and 15%. at 20% salt concentration no crystal formation was observed. calcite, aragonite and dolomite were precipitated in different quantities, depending on the salinity of the medium and incubation time. scanning and transmission electron microscopy, microanalysis and electro ... | 2000 | 10819230 |
| starch-hydrolyzing bacteria from ethiopian soda lakes. | alkaliphilic bacteria were isolated from soil and water samples obtained from ethiopian soda lakes in the rift valley area--lake shala, lake abijata, and lake arenguadi. starch-hydrolyzing isolates were selected on the basis of their activity on starch agar plate assay. sixteen isolates were chosen, characterized, and subjected to 16s rrna gene sequence analysis. all the isolates were gram positive and catalase- and beta-galactosidase positive. all isolates except one were motile endospore-formi ... | 2001 | 11354457 |
| nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline ekho lake, east antarctica, and emended description of the genus nesterenkonia. | an aerobic and heterotrophic isolate, designated ifam el-30t, was obtained from hypersaline ekho lake (vestfold hills, east antarctica). the isolate consisted of gram-positive cocci or short rods which occasionally exhibited branching. the organism was moderately halotolerant, required thiamin.hci and was stimulated by biotin and nicotinic acid. it grew well with glucose, acetate, pyruvate, succinate, malate or glutamate, and hydrolysed dna but not gelatin, starch or tween 80. nitrate was aerobi ... | 2002 | 12148619 |
| [occurrence of bacteria in the mouth from genera of micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus]. | the aim of the study was to assess the prevalence of different bacteria in the oral cavity. the bacteria were present in the oral cavities of 73 (48.7%) of 150 individuals. nesterenkonia halobia, the most frequently isolated species, was found in 20 (27%) individuals, micrococcus luteus in 16 (22%), kocuria kristinae in 12 (16%), kocuria varians in 10 (14%), dermacoccus sedentarius in 9 (12%), micrococcus lylae in 8 (11%), and kytococcus nishinomiyaensis in 3 (4%). mean counts of these microorga ... | 2002 | 12185681 |
| crystallization and preliminary x-ray analysis of an alkaline serine protease from nesterenkonia sp. | a novel calcium-independent serine protease from an alkaliphilic bacterium, nesterenkonia sp. al20, has been purified and crystallized at 296 k using sodium formate as the main precipitant. this enzyme is optimally active at ph 10, exhibits high stability towards autolytic digestion and its stability is not affected by the presence of edta or detergents. the triangular prism-shaped crystals diffracted x-rays to beyond 1.5 a at a synchrotron beamline, with space group r3 and unit-cell parameters ... | 2003 | 12595716 |
| [occurrence and number of bacteria from the micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus genera on skin and mucous membranes in humans]. | the aim of work was to evaluate the frequency of occurrence of bacteria from genera of micrococcus, kocuria, nesterenkonia, kytococcus and dermatococcus on human skin and mucous membranes in healthy population. among 150 investigated persons these bacteria were found in 122 (81.3%). the frequency of isolation was similar in both sex (82.4% in female and 79.7% in male). most often the strains were isolated from oral cavity (48.7%), and from skin of palm and forearm (40.7% and 37.3%). least freque ... | 2003 | 12908417 |
| [susceptibility to antibiotics of bacteria from genera micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus]. | two hundred and nineteen strains from genera micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus isolated from different sources, such as saliva, skin of palm and forearm and from vestibule of nose were tested. the susceptibility to doxycycline, cetriaxone, cefuroxyme, amikacyne, amoksycillin with clavulanic acid, trimethoprim/sulfamethoxazole, ampicillin, ciprofloxacin, penicillin and erythromycin were estimated. in general, bacteria from these genera are sensitive to most of select ... | 2003 | 12908418 |
| nesterenkonia halotolerans sp. nov. and nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of china. | the taxonomic position of two gram-positive strains, yim 70084(t) and yim 70097(t), isolated from hypersaline soils was determined by a polyphasic approach. cells of strain yim 70084(t) are motile cocci, whereas those of strain yim 70097(t) are non-motile rods. the g+c contents of their dna are 64.4 and 66.7 mol%. both strains had chemotaxonomic markers typical of the genus nesterenkonia and formed a coherent cluster with nesterenkonia species in a phylogenetic inference based on 16s rdna sequen ... | 2004 | 15143032 |
| bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. | heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ... | 2004 | 15574939 |
| nesterenkonia sandarakina sp. nov. and nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus nesterenkonia. | two novel actinobacteria isolates, designated yim 70009t and yim 70081t, were characterized in order to determine their taxonomic position. cells of strains yim 70009t and yim 70081t were cocci, although only the latter were motile. the g+c contents of their dnas were 64.0 and 64.5 mol%, respectively. on the basis of chemotaxonomic characteristics and 16s rrna gene sequence analysis, the two isolates were classified in the genus nesterenkonia. dna-dna hybridization and comparison of phenotypic c ... | 2005 | 15653919 |
| [study on isolation and systematic taxonomy of strains of genus nesterenkonia]. | four gram-positive, nesterenkonia-like strains were isolated from hypersaline soils collected from xinjiang province, china and east of egypt. the four strains were studies using a polyphasic approach, which included morphological observation, growth ph and temperature rang, tolerance to nacl, kcl, mgcl12 x 6h2o, cacl2, enzyme profiles, chemotaxonomy, (g + c) mol% content, 16s rdna sequences analysis and dna-dna hybridization, and the only two type strains of the genus nesterenkonia, nesterenkon ... | 2004 | 16110966 |
| diversity of alkaliphilic and alkalitolerant bacteria cultivated from decomposing reed rhizomes in a hungarian soda lake. | bacterial communities associated with decomposing rhizomes of phragmites australis were investigated in lake ferto (neusiedlersee, hungary). alkaliphilic and alkalitolerant strains were isolated on cellulose-containing alkaline medium spread with dilutions of scrapings taken from the surface of the decaying plant material. fifty-one strains were grouped by numerical analysis based on physiological tests and biolog sole carbon source utilization data. the strains identified by 16s rdna sequence c ... | 2005 | 16132425 |
| ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes. | the use of two-phase centrifugal decanters has been widely adopted in the olive oil extraction industry in order to reduce the huge quantities of wastewaters produced during the traditional three-phase extraction process. the resulting sludge-like byproduct, widely known as "alpeorujo", has a ph of 4-6, low water activity (a(w)) and high phytotoxicity. addition of ca(oh)(2) to alpeorujo, which is commonly performed at the olive oil mill to handle disposal problems related to acidic ph and odor e ... | 2006 | 16307869 |
| nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an ethiopian soda lake. | strain dsm 17733(t), isolated from the shore of lake abjata in ethiopia, is a heterotrophic, alkaliphilic, moderately halophilic, gram-positive, strictly aerobic, non-motile,non-endospore-forming bacterium. the organism grows optimally at 30-37 degrees c, ph 9 and 3 % (w/v) nacl. analysis of the cell wall showed the presence of murein of the type l-lys-gly-l-glu, variation a4alpha. the g + c content of the genomic dna was 69.0 mol%. sequence analysis of 16s rrna gene sequence of strain dsm 17733 ... | 2006 | 16738096 |
| nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional korean fermented seafood. | a gram-positive, non-motile, slightly halophilic actinomycete, strain jg-241t, was isolated from jeotgal, a traditional korean fermented seafood, and its taxonomic position was investigated by using a polyphasic approach. strain jg-241t grew optimally at 25-30 degrees c and in the presence of 2-5% (w/v) nacl. the physiological and biochemical properties of strain jg-241t were distinguishable from those of recognized nesterenkonia species. strain jg-241t had a peptidoglycan type based on l-lys-gl ... | 2006 | 17082396 |
| diversity of reed (phragmites australis) stem biofilm bacterial communities in two hungarian soda lakes. | from reed biofilm samples of kelemen-szék (kiskunság national park, knp) and nagy-vadas (hortobágy national park, hnp) altogether 260 bacterial isolates were gained after serial dilutions and plating onto different media. following a primary selection 164 strains were investigated by "traditional" phenotypic tests and clustered by numerical analysis. fifty-six representative strains were selected to ardra and 16s rdna sequence analysis for identification. strains were identified as members of ge ... | 2007 | 18088008 |
| nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil. | a gram-positive, non-motile, moderately halophilic, alkalitolerant actinobacterium, designated strain yim 70179(t), was isolated from a saline soil sample collected from xinjiang province, north-west china, and was subjected to a polyphasic taxonomic study. the cell-wall peptidoglycan type of strain yim 70179(t) was a4alpha, l-lys-gly-l-glu. cells of the isolate contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown glycolipid, mk-8 as major menaquinone and a ... | 2008 | 18523178 |
| cloning and characterization of the gene cluster for biosynthesis of ectoine from nesterenkonia halobia dsm 20541. | the ectabc genes encoding the biosynthesis of ectoine were identified from nesterenkonia halobia dsm 20541. the intergenic regions of the ectabc genes from n. halobia dsm 20541 were more loosely spaced than those that had been reported before. the amino acid sequence deduced from ectabc of the strain was highly homologous to the ectabc of brevibacterium linens bl2 (ecta 50%, ectb 70%, and ectc 68% identities). the osmoprotection of ectabc was studied in the escherichia coli knabc and e. coli xl1 ... | 2008 | 18604501 |
| genus-specific pcr for molecular identification of novel isolates of the genus nesterenkonia. | objective and method: for rapid identification of nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16s rrna gene sequences. results and conclusion: the genus specificity of these primers was validated with reference strains as well as with wild-type isolates. partial sequencing results of 16s rrna gene of the wild-type isolates confirmed that they are members of the genus nesterenkonia. | 2008 | 18652297 |
| nesterenkonia flava sp. nov., isolated from paper-mill effluent. | a gram-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated caas 251t, was isolated from paper-mill effluent in wuhan, china. the organism grew optimally at 40-42 degrees c and at ph 9.0-10.0. the major menaquinones were mk-7, mk-8 and mk-9. the predominant cellular fatty acids were anteiso-c15:0 (34.78 %), anteiso-c17:0 (25.24 %) and c16:0 (13.37 %). the g+c content of the genomic dna was 65.5 mol%. a phylogenetic analysis based on 16s rrna gene sequences showed that strain ... | 2008 | 18676482 |
| phylogenetic and metabolic bacterial diversity of phragmites australis periphyton communities in two hungarian soda ponds. | bacterial diversity of reed (phragmites australis) periphyton communities of kelemen-szék and nagy-vadas (two hungarian soda ponds) was investigated using molecular cloning and cultivation-based techniques. the majority of the 80 kelemen-szék and 72 nagy-vadas bacterial isolates proved to be moderately halophilic and alkaliphilic. a great proportion of the isolates showed phosphatase and urease activity, utilized aesculin, citrate and certain biopolymers (e.g., gelatine and tween 80). partial 16 ... | 2008 | 18679563 |
| crystal structure of an alkaline serine protease from nesterenkonia sp. defines a novel family of secreted bacterial proteases. | | 2008 | 18814301 |
| profiling of microbial communities in a bioreactor for treating hydrocarbon-sulfide-containing wastewater. | a technology of polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) was used to profile the structure and dynamic changes of microbial communities in a bioreactor for treating hydrocarbon-sulfide-containing (hsc) wastewater. the results showed that the heterotrophic genus of acinetobacter and the autotrophic genera of thiobacillus and thiomonas could survive well in all of three operating conditions. some special genera were also observed with changes of micro-ecoenviron ... | 2008 | 18817065 |
| nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. | an alkaliphilic actinobacterium, designated strain caas 252(t), was isolated from the black liquor treatment system of a cotton pulp mill in wuhan, china. cells of strain caas 252(t) were gram-positive, non-motile, non-endospore-forming, short rod-shaped, and grew optimally at 42 degrees c and ph 9-10 in the presence of 3 % (w/v) nacl. strain caas 252(t) contained mk-7, mk-8 and mk-9 as the major menaquinones and anteiso-c(17 : 0), anteiso-c(15 : 0) and c(16 : 0) as the predominant cellular fatt ... | 2009 | 19329621 |
| analysis of bacteria communities in an up-flow fixed-bed (ufb) bioreactor for treating sulfide in hydrocarbon wastewater. | an up-flow fixed-bed (ufb) bioreactor with patented functional polyurethane foam (fpuf) carriers was used to treat sulfide in hydrocarbon wastewater. community compositions of autotrophic and heterotrophic bacteria were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). dgge results showed that a relatively stable bacterial community composed of heterotrophic and autotrophic bacteria formed in the bioreactor by the end of experiment, which ensured 92-100% s ... | 2009 | 19559604 |
| gene cloning, expression, and characterization of a thermostable xylanase from nesterenkonia xinjiangensis cctcc aa001025. | an endo-beta-1,4-xylanase-encoding gene, xyn11nx, was cloned from nesterenkonia xinjiangensis cctcc aa001025 and expressed in escherichia coli. the gene encoded a 192-amino acid polypeptide and a putative 50-amino acid signal peptide. the deduced amino acid sequence exhibited a high degree of similarity with the xylanases from streptomyces thermocyaneoviolaceus (68%) and thermobifida fusca (66%) belonging to glycoside hydrolase family 11. after purification to homogeneity, the recombinant xyn11n ... | 2010 | 19838860 |
| purification and characterization of an organic-solvent-tolerant halophilic α-amylase from the moderately halophilic nesterenkonia sp. strain f. | a halophilic α-amylase produced by nesterenkonia sp. strain f was purified to homogeneity by 80% ethanol precipitation, q-sepharose anion exchange, and sephacryl s-200 gel filtration chromatography. the purified amylase exhibited specific activity of 357 unit/mg protein that corresponds to twofold purification. the molecular mass of the amylase was determined to be 57 kda by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and gel filtration chromatography. the optimal ph and ... | 2011 | 20593298 |
| diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania. | aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ... | 2010 | 20941581 |
| unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate. | nesterenkonia strain an1 was isolated from a screening program for nitrile- and amide-hydrolyzing microorganisms in antarctic desert soil samples. strain an1 showed significant 16s rrna sequence identity to known members of the genus. like known nesterenkonia species, strain an1 was obligately alkaliphilic (optimum environmental ph, 9 to 10) and halotolerant (optimum environmental na(+) content, 0 to 15% [wt/vol]) but was also shown to be an obligate psychrophile with optimum growth at approxima ... | 2011 | 21498772 |
| characterisation of the staphylococcal bacteriophage lysin chap(k). | aims: to develop an efficient purification strategy for the bacteriophage lysin chap(k) . to evaluate its antibacterial spectrum(,) enzymatic properties, optimal reaction conditions and lytic activity against live s. aureus. methods and results: recombinant chap(k) was purified to homogeneity by cation exchange chromatography, with yields of up to 10 mg from 1 litre of e. coli culture. the lytic spectrum of chap(k) includes all staphylococcal species and also members of the genera micrcococcus ... | 2011 | 21812876 |
| chemotactic responses to gas oil of halomonas spp. strains isolated from saline environments in argentina. | in this study, two halophilic bacterial strains isolated from saline habitats in argentina grew in the presence of gas oil. they were identified as halomonas spp. and nesterenkonia sp. by 16s ribosomal rna sequencing. chemotaxis towards gas oil was observed in halomonas spp. by using swimming assays. | 2011 | 21731972 |
| draft genome sequence of nesterenkonia sp. strain f, isolated from aran-bidgol salt lake in iran. | the draft genome of the aerobic, gram-positive, halophilic chemoorganotroph nesterenkonia sp. strain f consists of a 2,812,133-bp chromosome. this study is the first to report the shotgun-sequenced draft genome of a member of the genus nesterenkonia. | 2011 | 21914888 |
| microbial diversity and activity in hypersaline high arctic spring channels. | lost hammer (lh) spring is a unique hypersaline, subzero, perennial high arctic spring arising through thick permafrost. in the present study, the microbial and geochemical characteristics of the lh outflow channels, which remain unfrozen at ≥-18°c and are more aerobic/less reducing than the spring source were examined and compared to the previously characterized spring source environment. lh channel sediments contained greater microbial biomass (~100-fold) and greater microbial diversity reflec ... | 2012 | 22246205 |