| an indirect hemagglutination test for the diagnosis of eperythrozoon suis infection in swine. | an indirect hemagglutination test was developed to detect naturally occurring eperythrozoonosis in swine and to follow experimentally induced infections serologically. the antigen used was prepared from the plasma of acutely infected, splenectomized pigs. the test was specific and reliable in detecting swine latently infected with the disease. | 1975 | 1163869 |
| [the effect of eperythrozoon suis infection on the osmotic fragility of erythrocytes]. | osmotic fragility of erythrocytes was tested in weaned pigs experimentally infected with eperythrozoon (e.) suis. acute eperythrozoonosis of splenectomized pigs led to an increase of osmotic fragility. it is supposed that e. suis infection causes a structural change in erythrocyte membrane. possible mechanisms of this cell membrane injury are discussed. | 1992 | 1471973 |
| [light and electron microscopic studies of eperythrozoon suis]. | a light and electron microscopic study was carried out on pigs experimentally infected with eperythrozoon (e.) suis during the acute and chronic phase of infection. during the acute phase eperythrozoon organisms can be recognised by light microscopy as bright to dark orange points or circles. in contrast, in the chronically infected pigs e. suis appear as bright yellow or green small dots on the edge of the erythrocytes or in the plasma. by scanning electron microscopy, immature, juvenile and ma ... | 1992 | 1496521 |
| evaluation of an enzyme-linked immunosorbent assay for detection of eperythrozoon suis antibodies in swine. | an elisa was developed and tested to detect antibodies to eperythrozoon suis in swine. results were compared with those of the indirect hemagglutination (iha) test. antigen isolated from swine heavily infected with e suis was used for both tests. comparison of the elisa with the iha test revealed a significant (p less than 0.001) correlation between results. of 114 samples obtained from 9 swine infected with e suis, 87.7% were seropositive (titer greater than or equal to 200) via the elisa, and ... | 1992 | 1534474 |
| isolation of cold agglutinins in eperythrozoon suis-infected pigs. | a simple and reliable procedure is described for isolating the cold agglutinins associated with eperythrozoon suis-infection in swine. after initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using ficoll-paque, resuspended in rpmi-medium and warmed to 40 degrees c. the cold agglutinins could be removed from the supernatant by a subsequent centrifugation. double immunodiffusion, polyacrylamide gel electrophoresis and imm ... | 1992 | 1570680 |
| prevalence of pseudorabies virus infection and associated infections in six large swine herds in illinois. | sera were collected from 6 large farrow-to-finish swine herds infected with pseudorabies virus (prv) in illinois. all herds were participating in the large herd cleanup study, a usda-initiated project to evaluate the feasibility of eradicating pseudorabies from large farms (greater than 400 sows) by use of a combination of vaccination and management changes. herd size ranged between 425 and 1,500 breeding females. between april and july 1990, sera for measurement of prv antibodies were obtained ... | 1991 | 1651912 |
| in vitro and in vivo glucose consumption in swine eperythrozoonosis. | one complication of swine eperythrozoonosis is the hypoglycemia that occurs during parasitemia. to determine the cause of the hypoglycemia, we studied glucose consumption in splenectomized pigs infected with eperythrozoon suis. with the rapid rise of erythroparasites, the in vitro glucose consumption of parasited whole blood increased dramatically, and hypoglycemia developed. because mature porcine erythrocytes are impermeable to glucose, the increased glucose consumption is most logically the r ... | 1990 | 2220194 |
| detection of eperythrozoon suis dna from swine blood by whole organism dna hybridizations. | a procedure is described for isolating eperythrozoon suis dna of sufficient quantity and purity to serve as a probe in whole-organism dna hybridizations for detecting parasitized swine. the e. suis organisms were isolated from the blood of infected swine; the dna was recovered and digested with restriction endonucleases and resolved on agarose gels. in dna hybridizations using recovered e. suis dna, blood samples from parasitized swine could be differentiated from uninfected, control samples. a ... | 1990 | 2238458 |
| recombinant dna probe detecting eperythrozoon suis in swine blood. | a genomic library to eperythrozoon suis dna was constructed in lambda gt11, and from this library, e suis clone ksu-2 was identified as a potential diagnostic probe. in hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the ksu-2 probe hybridized strongly with purified e suis organisms and blood samples from splenectomized swine that were parasitized with e suis. however, the probe under stringent conditions did not give radiographic indi ... | 1990 | 2240802 |
| development of a recombinant anaplasma marginale dna probe. | an anaplasma marginale dna probe has been developed by using an improved method for the isolation of genomic dna. purified genomic a. marginale dna from the st. croix isolate was partially digested with sau 3a1 into fragments (greater than or equal to 5.0 kb). the restriction fragments were cloned using standard techniques in the pbr322 vector and used to transform e. coli (dh5) host cells. the recombinant a. marginale dna library was screened by the colony lifting procedure. colonies containing ... | 1990 | 2260276 |
| [the diagnosis of eperythrozoon suis infection]. | eperythrozoon infection can be diagnosed clinically and hematologically only during acute attacks. in the febrile phase it is possible to detect the eperythrozoon organisms in the blood smear. the best method for staining is that by acridine-orange and examination by fluorescence microscopy. serological identification (iha, kbr, elisa) is suitable for herd diagnosis only, because of the undulating level of the titre. in the individual case only positive results are regarded as significant. the b ... | 1990 | 2264051 |
| [disseminated intravascular coagulation in eperythrozoonosis of swine]. | investigations were carried out on the influence of latent and clinically manifest eperythrozoon suis infection upon haemostasis in swine. the study was carried out with 14 german landrace pigs. latent eperythrozoonosis was induced in 7 animals by experimental infection. splenectomy of these 7 animals and 2 spontaneously infected pigs led to clinical manifestation of eperythrozoonosis. five clinically healthy pigs were splenectomized and served as controls. in healthy pigs splenectomy was follow ... | 1990 | 2302156 |
| [serologic progression studies using cf and elisa for the detection of antibodies against eperythrozoon suis infection of swine]. | serological studies by means of a cf-test and elisa were undertaken for evaluation of antibody titers against eperythrozoon suis infection in swine. the animals were partly splenectomized and underwent artificial infection. it could be shown that the splenectomized animals showed higher and longer lasting antibody titers than the non splenectomized swine. it is stated, that the above mentioned tests can be used in diagnosis on a herd basis but not on a single animal basis. | 1990 | 2302160 |
| [eperythrozoon infection in swine: effect on the acid-base balance and the glucose, lactate and pyruvate content of venous blood]. | the influence of latent and of splenectomy-induced clinically manifest eperythrozoon suis infection on the following parameters of the carbohydrate metabolism and the acid-base status was tested in venous blood of german landrace pigs: levels of glucose, lactic and pyruvic acid, blood-ph, base excess, actual bicarbonate concentration, standard bicarbonate concentration, pco2, po2. the latent e. suis infection resulted in a consistent decrease of blood glucose level. 23 days after infection, bloo ... | 1990 | 2311527 |
| [the acid-base equilibrium and carbohydrate metabolism during infection with eperythrozoon suis]. | following on from clinical observations which point to severe metabolic disturbances in association with acute eperythrozoon (e.) suis infection, the parameters of acid-base balance (po2, pco2, ph, actual bicarbonate, standard bicarbonate, base excess) as well as the glucose-, lactate- and pyruvate levels, were measured in venous blood during the course of eperythrozoonotic infection. glucose consumption was investigated in in vitro experiments with differing numbers of pathogens. acute e. suis ... | 1990 | 2402971 |
| [eperythrozoon infection in swine as a disease factor]. | infection with eperythrozoon suis (e. suis) normally takes a latent course and depends on various factors. only animals suffering extreme stress show clinically manifest attacks with anemia, fever and occasionally jaundice. stress in the form of high animal-concentration in the stables, poor climatic conditions, change of stables or food or chronic general diseases predispose the pigs to clinical illness. the diagnosis of e. suis infection is made difficult by the prevalence of general diseases ... | 1989 | 2684152 |
| [indirect hemagglutination for eperythrozoon suis detection in experimentally and spontaneously infected swine]. | ten splenectomized and non-splenectomized pigs were experimentally infected with e. suis bearing red blood cells in order to determine the antibody response. all animals were monitored for antibody titer by indirect hemagglutination over a period of 80-290 days postinfection. latent e. suis infection only yielded a detectable antibody titer in one pig. acutely infected pigs had a titer ranging up to 1:640. maximum antibody response lasted only 2 months and dropped below the level of detection of ... | 1989 | 2800785 |
| isolation of infective and non-infective eperythrozoon suis bodies from the whole blood of infected swine. | | 1988 | 3218050 |
| [eperythrozoonosis in swine--an overlooked disease?]. | in the present work a review is given on the aetiology, clinical symptoms, prevalence and significance of the disease eperythrozoonosis in swine. clinical outbreaks are characterized by fever, anemia and icterus. clinical signs are most likely to be observed in young suckling piglets and in sows at the time of farrowing. in most cases infection with eperythrozoon suis leads to a carrier state of symptomless, subclinical infection which, however, may be activated under conditions of stress. epery ... | 1986 | 3523435 |
| experimental porcine eperythrozoonosis: t-lymphocyte suppression and misdirected immune responses. | immune responses and hematologic alterations were investigated in splenectomized pigs after im inoculation with eperythrozoon suis. early hematologic alterations were massive parasitism of rbc, severe hypoglycemia, moderate bilirubinemia, and mild anemia; later findings included severe anemia, minimal parasitism of rbc, spontaneous agglutination of rbc at 25 c and 4 c which was reversible at 37 c, transient thrombocytopenia, and mild bilirubinemia. the humoral immune responses consisting of a tr ... | 1985 | 3874572 |
| erythrocyte membrane alterations associated with the attachment and replication of eperythrozoon suis: a light and electron microscopic study. | erythrocytes from pigs with experimental porcine eperythrozoonosis were examined using light microscopy and scanning and transmission electron microscopy. three distinct forms of eperythrozoon suis were attached to the plasma surface of erythrocyte membrane. erythrocytes were initially parasitized by one or several immature forms. immature forms enlarged and developed into juvenile and mature forms. the parasite replicated by budding of small immature forms from larger immature forms, juvenile f ... | 1985 | 3984160 |
| [a contribution on splenectomy in swine]. | splenectomy as a diagnostic operation has gained great importance for discovering latent infection with eperythrozoon suis. the surgical technique is described. a specially developed thread ligature guide for the ligature of the a. and v. lienalis at the dorsal pole of the spleen is presented, that allows exact setting of the ligature. for maintaining deep anaesthesia the hypnotic etomidat is applicated by permanent infusion. | 1984 | 6528324 |
| pathogenicity of eperythrozoon suis alone and when mixed with babesia trautmanni in experimentally-infected pigs. | the pathogenicity of a single infection with eperythrozoon suis and of a mixed infection of e. suis and babesia trautmanni in experimentally-infected pigs, was described. in the pigs with a single infection, parasitaemia of e. suis was observed 4 days after blood inoculation. although parasitaemia with this parasite was also observed on the 4th day in a mixed infection, the parasitaemia of b. trautmanni was not noted until 14 days after inoculation at a period when that of e. suis had started to ... | 1983 | 6685398 |
| eperythrozoon suis in naturally infected pigs: a light and electron microscopic study. | the light and electron microscopic characteristics of natural infections with eperythrozoon suis are reported. e. suis are enclosed by a single membrane, and they reach a length of up to 600 nm and a diameter of 375 to 500 nm. neither distinct organelles nor nuclear structures appear in the parasites cytoplasm. they replicate by binary fission, and have close contact with red blood cells without showing any signs of membrance alteration. massive phagocytosis of whole red blood cells not parasiti ... | 1982 | 7147624 |
| studies on the blood parasites of pigs in ibadan, nigeria. | the blood parasites in the blood of local pigs kept under free range, and exotic pigs kept under intensive management of ibadan were investigated. the species found were babesia trautmanni, b. perroncitoi, eperythrozoon suis and e. parvum. eperythrozoon suis was the most predominant in both breeds. most of the local pigs carried mixed infections, while the same number of the exotic pigs carried single and mixed infections. mixed infection of individual pigs with all four blood parasites was comm ... | 1982 | 7201715 |
| [significance and course of a cold agglutinin in eperythrozoon suis infection of swine]. | after infection with eperythrozoon suis, pigs began to produce cold agglutinins of immunoglobulin type igm, that because of the similarity between the pathogenic antigen and antigen on the erythrocyte membrane caused agglomeration. the progression of this cold agglutinin was measured with an enzyme immunoassay especially invented for this purpose, and compared with serum-igm, agglutination strength, pathogenic effect as well as number of erythrocytes in the blood. the cold agglutinin extracted f ... | 1994 | 7945184 |
| comparison of an improved polymerase chain reaction protocol and the indirect hemagglutination assay in the detection of eperythrozoon suis infection. | the ability of an improved polymerase chain reaction (pcr) protocol to detect eperythrozoon suis dna in the blood of experimentally infected nonsplenectomized pigs was evaluated. the protocol utilizes previously described e. suis-specific primers and a proprietary dna-releasing reagent in a 2-step amplification cycle followed by visualization of the 492-bp amplification product on agarose gels. this pcr protocol successfully amplified e. suis dna in blood from all postinfection samples and from ... | 1994 | 7948201 |
| eperythrozoon suis in weaned pigs. | | 1994 | 8135021 |
| in situ hybridizations of eperythrozoon suis visualized by electron microscopy. | eperythrozoon suis is an extracellular red blood cell parasite that causes icteroanemia and poor growth performance in feeder pigs and has been associated with anemias in baby pigs and reproductive failures in sows. at present, few efficient tests are available for the diagnosis and study of e. suis infection in swine. this report discusses how a recently developed recombinant dna probe (ksu-2) specific to e. suis dna was utilized in in situ dna hybridizations that couple biotinylated taqi diges ... | 1993 | 8236784 |
| on the transmissibility of eperythrozoon suis by stomoxys calcitrans and aedes aegypti. | the stable fly, stomoxys calcitrans (linnaeus), and the yellow fever mosquito, aedes aegypti (linnaeus), were utilized to determine their capability to transmit eperythrozoon suis splitter between swine. three groups of each insect in each trial were allowed to feed on a pig previously infected with e. suis and then transferred to susceptible splenectomized pigs. as a control, one group of each insect was fed on a non-infected pig and then transferred to a susceptible pig. stable flies were tran ... | 1993 | 8291187 |
| experimental infections and natural outbreaks of eperythrozoonosis in pigs identified by pcr-dna hybridizations. | eperythrozoon-specific dna amplification reactions and subsequent hybridizations with an eperythrozoon dna probe (ksu-2) were used in experimental infection studies to identify eperythrozoon suis dna in the blood of splenectomized and nonsplenectomized pigs. the results indicate that e. suis dna is present in nonsplenectomized pigs at levels that can be amplified by polymerase chain reaction (pcr) and identified in dna hybridizations within 24 hours after infection. the ability of the e. suis pc ... | 1993 | 8373846 |
| detection of eperythrozoon suis using the polymerase chain reaction. | eperythrozoon suis is an extracellular red blood cell parasite that causes icteroanemia in acutely ill pigs and a variety of syndromes in chronically infected pigs. current techniques to detect e. suis infection are limited by variability of parasitemias and antibody responses in infected animals. the polymerase chain reaction (pcr) was investigated to determine its potential as a means of detecting e. suis infection in pigs. with dna samples extracted from either purified e. suis organisms or e ... | 1993 | 8466979 |
| effect of immunosuppressive treatment on eperythrozoon suis infection and porcine peripheral-blood natural-killer-(nk) cell activity. | the effects of immunosuppressive treatment on natural-killer-cell activity and the manifestation of eperythrozoonosis in swine were investigated. after infection with eperythrozoon suis (e. suis), german landrace piglets received daily intravenous (i.v.) applications of cyclophosphamide (cy, endoxan) on 6 consecutive days. a combined immunosuppressive treatment using azathioprine (imurek), a single application of dexamethasone (devan), and two injections of anti-asialo gm1 serum (1:50) was perfo ... | 1995 | 8592905 |
| in vitro maintenance of eperythrozoon suis. | in vitro maintenance of eperythrozoon suis was attempted using a petri dish erythrocyte culture system. in preliminary experiments, the optimal conditions for maintaining e. suis attachment to erythrocytes during incubation were anticoagulation with heparin or citrate solution, incubation with 5 or 10% co2 at 37 degrees c, and incubation with reduced or non-reduced eagle's minimum essential medium. using heparin, a co2 incubator and reduced eagle's medium (rem), e. suis metabolic activity was ev ... | 1996 | 8720557 |
| anaemia and low viability in piglets infected with eperythrozoon suis. | eperythrozoon suis infection was identified in a pig herd during an investigation into anaemia and low viability in newborn piglets and severe regenerative macrocytic anaemia in older piglets. the organisms were identified in the erythrocytes of piglets a few days old. extensive investigations failed to detect other causes of the anaemia and low viability. there was no response to parenteral iron administration alone but the piglets' viability and anaemia responded to the administration of tetra ... | 1997 | 9050175 |
| western immunoblot analysis of haemobartonella muris and comparison of 16s rrna gene sequences of h. muris, h. felis, and eperythrozoon suis. | infectious agents were isolated from the spleens of three wild mice (apodemus argenteus) by intraperitoneal inoculation of the spleen homogenate into laboratory mice. the laboratory mice developed clinical signs and splenomegaly, and three isolates were maintained by passage in mice. tetracyclines were effective in preventing infection of mice with these agents, but streptomycin and penicillin were ineffective. the agents did not grow in bacterial growth media or chicken embryos. in smears of bl ... | 1997 | 9157135 |
| development and evaluation of a pcr-based assay for detection of haemobartonella felis in cats and differentiation of h. felis from related bacteria by restriction fragment length polymorphism analysis. | the 16s rrna gene of haemobartonella felis was amplified by using universal eubacterial primers and was subsequently cloned and sequenced. based on this sequence data, we designed a set of h. felis-specific primers. these primers selectively amplified a 1,316-bp dna fragment of the 16s rrna gene of h. felis from each of four experimentally infected cats at peak parasitemia. no pcr product was amplified from purified dna of eperythrozoon suis, mycoplasma genitalium, and bartonella bacilliformis. ... | 1998 | 9466759 |
| development and evaluation of a polymerase chain reaction assay using the 16s rrna gene for detection of eperythrozoon suis infection. | the 16s ribosomal rna (rrna) gene of eperythrozoon suis was amplified using gene-specific primers developed from genbank sequence accession u88565. the gene was subsequently cloned and sequenced. based on these sequence data, 3 sets of e. suis-specific primers were designed. these primers selectively amplified 1394, 690, and 839 base-pair (bp) fragments of the 16s rrna gene from dna of e. suis extracted from the blood of an experimentally infected pig during a parasitemic episode. no polymerase ... | 1999 | 10353353 |
| genome size of eperythrozoon suis and hybridization with 16s rrna gene. | the genome size of eperythrozoon suis, an unculturable haemotropic mycoplasma, was estimated using pulsed-field gel electrophoresis (pfge). gamma irradiation was used to introduce one (on the average) double-strand break in the e. suis illinois chromosome. restriction enzymes that cut infrequently were also used to analyze genome size. the size estimate for the full-length genome was 745 kilobases (kb), whereas the size estimates based on the summation of restriction fragments ranged from 730 to ... | 2000 | 11109499 |
| 'candidatus mycoplasma haemominutum', a low-virulence epierythrocytic parasite of cats. | the phylogenetic position and some taxonomically relevant characteristics of a small, low-virulence bacterial parasite of cats are described. a 16s rdna analysis revealed that the organism was in the mycoplasma clade and was most closely related to a parasite of pigs previously designated eperythrozoon suis. as the organism has not been cultured in vitro and is maintained in serial passage in cats in vivo, candidatus status is proposed for this novel taxon as 'candidatus mycoplasma haemominutum' ... | 2001 | 11411702 |
| proposal to transfer some members of the genera haemobartonella and eperythrozoon to the genus mycoplasma with descriptions of 'candidatus mycoplasma haemofelis', 'candidatus mycoplasma haemomuris', 'candidatus mycoplasma haemosuis' and 'candidatus mycoplasma wenyonii'. | cell-wall-less uncultivated parasitic bacteria that attach to the surface of host erythrocytes currently are classified in the order rickettsiales, family anaplasmataceae, in the genera haemobartonella and eperythrozoon. recently 16s rrna gene sequences have been determined for four of these species: haemobartonella felis and haemobartonella muris and eperythrozoon suis and eperythrozoon wenyonii. phylogenetic analysis of these sequence data shows that these haemotrophic bacteria are closely rel ... | 2001 | 11411711 |
| revision of haemotrophic mycoplasma species names. | the recently proposed transfer of four rickettsias from the genera haemobartonella and eperythrozoon to the genus mycoplasma with the candidatus status is herein revised. this is because the candidatus designation is for new, incompletely described taxa, in order to give them a provisional status. thus, 'candidatus mycoplasma haemofelis' is revised to mycoplasma haemofelis comb. nov., nom. nov., 'candidatus mycoplasma haemomuris' is revised to mycoplasma haemomuris comb. nov., nom. nov., 'candid ... | 2002 | 11931184 |
| development of a diagnostic pcr assay based on novel dna sequences for the detection of mycoplasma suis (eperythrozoon suis) in porcine blood. | an efficient method of control of porcine eperythrozoonosis (pe) caused by mycoplasma suis is eradication of infection by detection and removal of infected carrier animals. at present, only a few tests are available for the diagnosis of these latent m. suis infections in pigs. the objective of this study was to develop a pcr assay based on novel dna sequences for the identification of m. suis-infected pigs. a 1.8 kb ecori dna fragment of the m. suis genome was isolated from the blood of pigs exp ... | 2003 | 12695043 |
| a picture of eperythrozoon suis. | | 1958 | 13490032 |
| eperythrozoon suis n. sp. and eperythrozoon parvum n. sp., 2 new blood parasites of swine. | | 1950 | 15418178 |
| eperythrozoon suis, the etiologic agent of ictero-anemia or an anaplasmosis-like disease in swine. | | 1950 | 15425763 |
| development of in-situ hybridization for the detection of mycoplasma haemosuis (eperythrozoon suis) in formalin-fixed, paraffin wax-embedded tissues from experimentally infected splenectomized pigs. | mycoplasma haemosuis dna was detected in experimentally infected splenectomized pigs by in-situ hybridization (ish) with a nonradioactive digoxigenin-labelled dna probe. an 839 base pair dna probe targeting a 16s rrna gene was generated by the polymerase chain reaction. eight 6-week-old pigs were inoculated intraperitoneally with 6 ml of m. haemosuis-infected pig blood and eight negative control pigs were inoculated intraperitoneally with 6 ml of m. haemosuis-free blood. two pigs from each group ... | 2005 | 16212975 |
| mycoplasma suis antigens recognized during humoral immune response in experimentally infected pigs. | today, serodiagnostic tests for mycoplasma suis infections in pigs have low accuracies. the development of novel serodiagnostic strategies requires a detailed analysis of the humoral immune response elicited by m. suis and, in particular, the identification of antigenic proteins of the agent. for this study, indirect enzyme-linked immunosorbent assay (elisa) and immunoblot analyses were performed using pre- and sequential postinoculation sera from m. suis-infected and mock-infected control pigs. ... | 2006 | 16426008 |
| eperythrozoon infection in young bulls with scrotal and hindlimb edema, a herd outbreak. | young (9 to 10 mo) aberdeen-angus bulls (n = 5) in excellent body condition and pastured with approximately 35 other bulls of similar age on a farm in southeastern alabama had fever, harsh pulmonary sounds, increased respiratory rate and variable amounts of hindlimb and scrotal edema. bulls had mild microcytic, normochromic anemia. numerous eperythrozoon organisms were identified on blood smears. indirect hemagglutination inhibition test results for eperythrozoon suis antibodies were negative ei ... | 1995 | 16727647 |
| identification of a haemomycoplasma species in anemic reindeer (rangifer tarandus). | during an 18-mo period (may 2002-november 2003), 10 animals in a herd of 19 reindeer (rangifer tarandus) at the national animal disease center (nadc) experienced episodes of anemia. affected animals had histories of weight loss, unthriftiness, occasionally edema of dependent parts and moderate anemia characterized by microcytosis or macrocytosis, hypochromasia, schistocytosis, keratocytosis, acanthocytosis, and dacryocytosis. numerous basophilic punctate to ring-shaped bodies, measuring less tha ... | 2006 | 16870847 |
| porcine eperythrozoonosis in china. | eperythrozoonosis of swine (also designated as porcine mycoplasmosis) is a disease of swine under stress, expressed as a febrile condition with development of an acute ictero-anemia. it is caused by eperythrozoon suis and usually causes a subclinical infection with a latent carrier state that persists for extended periods. in china, this disease has gradually developed as an important intercurrent disease and an emerging swine disease that, in recent years, has spread throughout all provinces ex ... | 2006 | 17135527 |
| [detection of mycoplasma suis in clinical cases with postweaning multisystemic wasting syndrome in swine]. | mycoplasma suis is a swine erythrocyte obligatory parasite. its presence may result in chronic or acute anaemia in different pig categories. it is considered that the postweaning multisystemic wasting syndrome (pmws) is caused by porcine circovirus type 2, but some aspects of the pathogenesis remain unknown. pmws signs are impaired weight gain, anaemia and jaundice in 5 to 12 week-old pigs that suffer from immunosuppression and bacterial co-infections. the pigs with signs of these diseases on th ... | 2006 | 17152209 |
| exploratory study of mycoplasma suis (eperythrozoon suis) on four commercial pig farms in southern brazil. | mycoplasma suis (eperythrozoon suis) was detected by pcr and southern blot in 186 pigs (121 sows, 61 piglets and four boars) on four farms in southern brazil. dna was extracted from blood samples and a 16s rrna gene fragment of m suis was amplified by pcr; southern blot analysis was then performed on all the samples. twenty-two of the sows (18.2 per cent) were positive by pcr, and 40 (33.1 per cent) were positive by southern blot; only one piglet and one boar were positive. the packed cell volum ... | 2007 | 17220522 |
| effect of an immunosuppressor (dexamethasone) on eperythrozoon infection. | the aim was to study the relationship between the infection rate of eperythrozoon suis and the status of the immune system. four pigs with chronic eperythrozoonosis were divided into experimental and control groups. immunosuppression of the experimental group was accomplished by injecting with high-dose glucocorticoid (dexamethasone) on 3 consecutive days. microscopic examination of blood smears was performed to observe the change of infection rate after administration. the blood samples of each ... | 2007 | 17287887 |
| [significance of haemotrophic mycoplasmas in veterinary medicine with particular regard to the mycoplasma suis infection in swine]. | phylogenetic investigations led to the definition of a new group of bacteria called haemotrophic mycoplasmas (haemoplasmas). the life cycle of said globally spread bacteria is dependent on their intimate contact with their host erythrocytes. illnesses showing symptoms of a haemolytic anaemia are usually found in cats and swine. haemoplasmas are small pleomorphic bacteria (0.3-3 microm) with a very small genome (745-1245 kbp). to date there is very limited knowledge on their biology and the host- ... | 2007 | 17290941 |
| first identification and functional characterization of an immunogenic protein in unculturable haemotrophic mycoplasmas (mycoplasma suis hspa1). | the antigenic structures of the haemotrophic mycoplasma suis, an epicellular parasite of porcine erythrocytes, are largely unknown due to its unculturability. in this study, serological proteome and mass spectrometry analyses allowed the characterization of m. suis proteins targeted by the porcine antibody response: two proteins with characteristics of heat shock proteins, two proteins with characteristics of glycolytic enzymes, a rna helicase- and an actin-like protein. the dnak-like protein of ... | 2007 | 17328755 |
| msg1, a surface-localised protein of mycoplasma suis is involved in the adhesion to erythrocytes. | mycoplasma suis is a member of the group of uncultivable haemoplasmas which colonise erythrocytes of a wide range of vertebrates. adhesion to erythrocytes is the crucial step in the unique haemoplasma life cycle. due to the lack of a cultivation system, no adhesion structures have been identified so far. in order to determine potential adhesion molecules of m. suis, we screened genomic m. suis libraries. the protein msg1 with glyceraldehyde-3-phosphate dehydrogenase (gapdh) similarity was identi ... | 2007 | 17336568 |
| first lightcycler real-time pcr assay for the quantitative detection of mycoplasma suis in clinical samples. | mycoplasma suis cannot be cultivated in vitro. therefore, pcr-based methods are irreplaceable for the diagnosis of m. suis infections especially when clinical symptoms are not evident. currently, no easy and reliable method allowing the quantitative detection of m. suis is available. this report describes the development of a quantitative lightcycler pcr assay based on the msg1 gene of m. suis (lc msg1 pcr). no pcr signals were obtained with closely related haemotrophic and non-haemotrophic myco ... | 2007 | 17586075 |
| use of recombinant antigens to detect antibodies against mycoplasma suis, with correlation of serological results to hematological findings. | porcine eperythrozoonosis is a disease with worldwide distribution caused by the unculturable hemotrophic bacterium mycoplasma suis. current serological testing utilizes crude m. suis antigens purified from the blood of experimentally infected pigs. these antigens show high variability and are restricted to specialized laboratories. we evaluated a novel serological assay based on two recombinant m. suis antigens (rmsg1 and rhspa1). antigen specificity was proven by means of sera raised against n ... | 2007 | 17942612 |
| haemotrophic mycoplasmas: recent advances in mycoplasma suis. | haemotrophic mycoplasmas (haemoplasmas) are uncultivable, small epicellular, cell wall less, tetracycline-sensitive bacteria that attach to the surface of host erythrocytes. today, haemotrophic mycoplasmas are found in a large number of animals, with mycoplasma suis being the porcine pathogen. haemoplasmas can cause infections which are clinically marked, either by an overt life-threatening haemolytic anaemia or a mild chronic anaemia, by illthrift, infertility, and immune suppression. the life ... | 2008 | 18358641 |
| prevalence of mycoplasma suis in slaughter pigs, with correlation of pcr results to hematological findings. | porcine infectious anemia is a well-known disease that occurs worldwide and is caused by the unculturable hemotrophic bacterium mycoplasma suis. the actual prevalence and impact of m. suis infections, however, remain fairly unknown. this study examined the prevalence of m. suis in post-weaning pigs by employing a quantitative real-time lightcycler pcr. m. suis infections were detected in 164 out of 1176 feeder pigs (20-30 kg; 13.9%) as well as on 79 out of 196 pig farms (40.3%). the comparison o ... | 2009 | 18687536 |
| establishment of an efficient enzyme-linked immunosorbent assay for the detection of eperythrozoon sius antibody in swine. | the eperythrozoon suis (e. suis) antigen was purified using a sephadex g-200 chromatograph, and thereby, a high-affinity, specific e. suis antigen was collected and confirmed with western blotting. using this antigen, an enzyme-linked immunosorbent assay (elisa) system to detect the antibody against e. suis in swine was established. there was no cross-reaction with swine sera, which were affected with mycoplasmal pneumonia, swine fever, swine colibacillosis, or toxoplasmosis. a comparison of thi ... | 2008 | 18981677 |
| mycoplasma suis invades porcine erythrocytes. | mycoplasma suis belongs to the hemotrophic mycoplasma group and causes infectious anemia in pigs. according to the present state of knowledge, this organism adheres to the surface of erythrocytes but does not invade them. we found a novel m. suis isolate that caused severe anemia in pigs with a fatal disease course. interestingly, only marginal numbers of the bacteria were visible on and between the erythrocytes in acridine orange-stained blood smears for acutely diseased pigs, whereas very high ... | 2009 | 19015255 |
| the 1.8kb dna fragment formerly confirmed as mycoplasma suis (m. suis) specific was originated from the porcine genome. | | 2009 | 19304412 |
| prevalence of mycoplasma suis (eperythrozoon suis) infection in swine and swine-farm workers in shanghai, china. | to determine the prevalence of mycoplasma suis infection in swine, swine-farm workers, and swine veterinarians in shanghai, china. | 2009 | 19566474 |
| phylogenetic analysis of mycoplasma suis isolates based on 16s rrna gene sequence in china. | to perform phylogenetic analysis of mycoplasma suis isolates derived from china to define the nature of this pathogen, nearly complete of 16s rrna genes from chongqing, sichuan, henan and guangdong isolates were amplified by pcr and sequenced. the four sequences from the blood samples in this study, with other 17 hemoplasmas sequences and related 3 mycoplasma sequences available in the genbank, were aligned using clustal x (version 1.83) sequences alignment program. maximum parsimony, neighbor-j ... | 2009 | 19590972 |
| vaccination with the mycoplasma suis recombinant adhesion protein msg1 elicits a strong immune response but fails to induce protection in pigs. | mycoplasma suis is the unculturable pathogen of porcine infectious anemia. the study was aimed to determine the immunogenicity and protective efficacy of msg1, an immunodominant adhesin of m. suis as the first vaccine candidate against m. suis. the results demonstrated that recombinant msg1 and escherichia coli transformants expressing msg1 (e. coli_msg1) induced a strong humoral and cellular immunity against m. suis. the induced antibodies were found to be functionally active as confirmed by an ... | 2009 | 19596408 |
| a review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in brazil. | recent studies have been conducted in brazil using molecular techniques for the detection of hemotrophic mycoplasmas in several mammals. in domestic cats, mycoplasma haemofelis, "candidatus m. haemominutum", and "candidatus m. turicensis" infections have been identified. these species have also been found in free-ranging and captive neotropical felid species. two canine hemoplasmas, mycoplasma haemocanis and "candidatus mycoplasma haematoparvum", have been identified in dogs. in commercial swine ... | 2009 | 19772768 |
| proteomic study of mycoplasma suis using the gel-based shotgun strategy. | mycoplasma suis (m. suis) is an uncultivable pathogen that colonizes the surface of porcine erythrocytes. in the present study, gel-based lc-ms/ms was performed to analyze the proteomic composition of m. suis. we identified 191 proteins with two or more peptides and additional 217 putative proteins with one peptide hit by cross-species searching the mollicutes protein databases. resultant proteins were classified based on their molecular functions. the majority of enzymes involved in central car ... | 2010 | 19969430 |
| occurrence of mycoplasma suis in wild boars (sus scrofa l.). | porcine infectious anemia is a well-known disease that occurs worldwide and is caused by the uncultivable hemotrophic bacterium mycoplasma suis. in this study the occurrence of m. suis in wild boars was investigated by employing a quantitative real-time lightcycler pcr. m. suis infections were detected in 36 out of 359 wild boars (10.03%). sequencing of the 16s rrna gene and subsequent phylogenetic analysis revealed the existence of two genetically distinct m. suis subtypes in the wild boar popu ... | 2010 | 20006452 |
| antibodies to actin in autoimmune haemolytic anaemia. | in autoimmune haemolytic anaemia (aiha), autoreactive antibodies directed against red blood cells are up-regulated, leading to erythrocyte death. mycoplasma suis infections in pigs induce aiha of both the warm and cold types. the aim of this study was to identify the target autoantigens of warm autoreactive igg antibodies. sera from experimentally m. suis-infected pigs were screened for autoreactivity. | 2010 | 20353574 |
| inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from mycoplasma suis. | mycoplasma suis belongs to a group of highly specialized hemotrophic bacteria that attach to the surface of host erythrocytes. hemotrophic mycoplasmas are uncultivable and the genomes are not sequenced so far. therefore, there is a need for the clarification of essential metabolic pathways which could be crucial barriers for the establishment of an in vitro cultivation system for these veterinary significant bacteria.inorganic pyrophosphatases (ppase) are important enzymes that catalyze the hydr ... | 2010 | 20646294 |
| complete genome sequences of two hemotropic mycoplasmas, mycoplasma haemofelis strain ohio2 and mycoplasma suis strain illinois. | we report the complete and fully assembled genomes of mycoplasma haemofelis strain ohio2 and mycoplasma suis strain illinois, which are the first available genomes of these uncultivatable hemoplasma species. the single circular chromosomes of 1,152,484 bp and 742,431 bp for m. haemofelis and m. suis, respectively, are typical of mycoplasma species, having reduced size and low g+c content (38.8% for m. haemofelis and 31.1% for m. suis). their metabolic pathways are reduced, with evidence of adapt ... | 2011 | 21317328 |
| complete genome sequence of the hemotrophic mycoplasma suis strain ki3806. | mycoplasma suis, a member of the hemotrophic mycoplasma (hm) group, parasitize erythrocytes of pigs. increasing evidence suggests that m. suis is also a zoonotic agent. highly pathogenic strains of m. suis (e.g., m. suis ki3806) have been demonstrated to invade erythrocytes. this complete sequenced and manually annotated genome of m. suis ki3806 is the first available from this species and from the hm group. the dna was isolated from blood samples of experimentally infected pigs due to the lack ... | 2011 | 21398558 |
| synthesis, cloning, and expression of mycoplasma suis inorganic pyrophosphatase gene using pcr-based accurate synthesis and overlap-extension pcr, and its immunogenicity analysis. | mycoplasma suis (m. suis), a hemotrophic pathogen of pigs, causes economic losses in swine production throughout the world. inorganic pyrophosphatase (ppa) is a very important gene in m. suis. the ppa gene of m. suis was synthesized by pcr-based accurate synthesis (pas) and overlapextension pcr, inserted into vector pmd18-t, and then subcloned to the prokaryotic expression vector pet28c.the recombinant plasmid pet28c_ppa was transformed to e. coli bl21 for expression under induction of isopropyl ... | 2011 | 21429540 |
| use of a mycoplasma suis-pcr protocol for screening a population of captive peccaries (tayassu tajacu and tayassu pecari). | mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (pcr) of the 16s rna gene of m. suis improves the sensitivity and specificity of detection. the aim of this study was to screen peccaries (tayassu tajacu and t. pecari) for m. suis infection using a specific conventional pcr. a total of 28 blood samples from captiv ... | 2011 | 21439238 |
| complete genome sequence of mycoplasma suis and insights into its biology and adaption to an erythrocyte niche. | mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. thus, the objective herein was to use whole genome sequencing and analysis of m. suis to define pathogenicity mechanisms and biochemical pathways. m. suis was harvested from the blood of an experimentally infected pig. following dna extraction and construction of a paired end library, whole-genome sequencing was performed using gs-fl ... | 2011 | 21573007 |
| a quantitative taqman pcr assay for the detection of mycoplasma suis. | aim: to develop a taqman probe-based, highly sensitive and specific quantitative pcr (qpcr) assay for the detection and quantification of m. suis in the blood of pigs. methods and results: primers and probes specific to m. suis 16s rrna gene were designed. the qpcr assay's specificity, detection limit, intra- and inter-assay variability were evaluated and its performance was compared to a m. suis conventional pcr assay (cpcr). blood of two experimentally infected pigs, 40 indiana pigs, 40 braz ... | 2011 | 21575112 |
| two genetic clusters in swine hemoplasmas revealed by analyses of the 16s rrna and rnase p rna genes. | only two hemoplasma species, eperythrozoon parvum and mycoplasma suis, have been recognized in pigs. here we demonstrate the genetic variations among six hemoplasma strains detected from pigs, by analyzing the 16s rrna and rnase p rna (rnpb) genes, and propose a novel hemoplasma taxon that has not been described previously. phylogenetic trees based on the nucleotide sequence of the 16s rrna gene indicated that these six hemoplasmas were divided into two clusters representing m. suis and a novel ... | 2011 | 21799294 |
| molecular characterization of the uncultivatable hemotropic bacterium mycoplasma haemofelis. | abstract: mycoplasma haemofelis is a pathogenic feline hemoplasma. despite its importance, little is known about its metabolic pathways or mechanism of pathogenicity due to it being uncultivatable. the recently sequenced m. haemofelis str. langford 1 genome was analysed and compared to those of other available hemoplasma genomes.analysis showed that in hemoplasmas genes involved in carbohydrate metabolism are limited to enzymes of the glycolytic pathway, with glucose appearing to be the sole ene ... | 2011 | 21749699 |
| Hemotrophic mycoplasmas induce programmed cell death in red blood cells. | Hemotrophic mycoplasmas (HM) are uncultivable bacteria found on and in the red blood cells (RBCs). The main clinical sign of HM infections is the hemolytic anemia. However, anemia-inducing pathogenesis has not been totally clarified. In this work we used the splenectomized pig as animal model and Mycoplasma suis as a representative for hemotrophic mycoplasmas to study anemia pathogenesis. Eryptosis, i.e. programmed cell death of RBCs, is characterized by cell shrinkage, microvesiculation and pho ... | 2011 | 21691073 |
| the surface-localised α-enolase of mycoplasma suis is an adhesion protein. | mycoplasma suis belongs to the haemotrophic mycoplasmas which colonise red blood cells of a wide range of vertebrates. adhesion to red blood cells is the crucial step in the unique lifecycle of m. suis. due to the lack of a cultivation system, identification of adhesion structures has been difficult. so far, only one adhesion protein, i.e. msg1 was identified. in order to determine further adhesion molecules of m. suis, we screened genomic m. suis libraries and performed southern blot hybridisat ... | 2011 | 22047714 |
| [porcine eperythrozoonosis: from eperythrozoon suis to mycoplasma suis]. | mycoplasma suis (formerly known as eperythrozoon suis ) is the most prevalent agent causing haemolytic anaemia in swine. the disease is also known as porcine eperythrozoonosis. m.suis is a small, pleomorphic bacteria parasitizing porcine erythrocytes. to date, no in vitro cultivation system for m.suis has been established and, therefore, our knowledge about the characteristics of m.suis and the pathogenesis of porcine eperythrozoonosis is rather limited. m.suis can cause acute disease, ... | 2011 | 22138829 |
| development and evaluation of enzyme-linked immunosorbent assay based on recombinant inorganic pyrophosphatase gene antigen for the detection of mycoplasma suis antibodies. | the recombinant ppa protein of mycoplasma suis migrated to 21kda. using this antigen, an elisa system to detect the antibody against m. suis infection in swine was established. the relisa demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with m. suis infection. a comparison of this elisa system with an indirect hemagglutination assay (iha) test using 132 swine samples revealed that the positive rate was 34.0% in elisa and 28.0% in iha. compared ... | 2011 | 21940024 |
| detection of mycoplasma haemocanis, mycoplasma haematoparvum, mycoplasma suis and other vector-borne pathogens in dogs from córdoba and santa fé, argentina. | in argentina, only very few reports are available for canine tick-borne diseases where most are related to parasitic diseases. the objective of this survey was to investigate the prevalence of tick-borne pathogens in 70 dogs from santa fé and córdoba, argentina. | 2016 | 27978844 |
| development of colloidal gold-based immunochromatographic assay for rapid detection of mycoplasma suis in porcine plasma. | a one-step immunochromatographic assay using gold nanoparticles coated with polyclonal antibody (pab) against mycoplasma suis (m. suis) was developed in this study for the detection of m. suis in porcine plasma. the colloidal gold was prepared by the reduction of gold salt with sodium citrate coupled with pab against m. suis. the pab was produced by immunizing the balb/c mice with recombinant msg1 (rmsg1) protein from m. suis expressed in escherichia coli. the optimal concentrations of the captu ... | 2014 | 24434494 |
| identification of b cell epitopes in the msg1 protein of mycoplasma suis. | mycoplasma suis (m. suis) is an extracellular bacterial organism that attaches to and causes deformity and damage to porcine red blood cells. m. suis glyceraldehyde-3-phosphate dehydrogenase-like protein 1 (msg1), a membrane-associated adhesion protein, plays a major role in m. suis attachment and infection of porcine erythrocytes. in order to identify the epitopes in msg1 protein of m. suis, recombinant msg1 (rmsg1) expressed in escherichia coli top10 was purified with affinity chromatography a ... | 2014 | 25171002 |
| use of msg1 protein in a novel blocking elisa for the detection of mycoplasma suis infection. | a blocking elisa based on the monoclonal antibody (mab) 1a7 was developed to detect antibodies against mycoplasma suis. the mab was produced by immunising balb/c mice with recombinant msg1 protein (rmsg1) from m. suis expressed in e. coli. following identification by western blotting, the mab was purified and labelled with horseradish peroxidase. the parameters of the elisa were optimised, and the cut-off value determined as 36.35% by analysing the percentage inhibition of m. suis negative serum ... | 2012 | 22285443 |
| genome sequence of mycoplasma parvum (formerly eperythrozoon parvum), a diminutive hemoplasma of the pig. | we report the complete genome sequence of mycoplasma parvum strain indiana. its circular chromosome is 564,395 bp, which is smaller than that of mycoplasma genitalium, which was previously considered the smallest member of the mollicutes. comparative analyses of the genomes of m. parvum and mycoplasma suis will provide novel insights into the molecular basis of their virulence. | 2013 | 24285648 |
| occurrence and molecular characterization of bartonella spp. and hemoplasmas in neotropical primates from brazilian amazon. | little is known about the prevalence and genetic diversity of bartonella spp. and hemoplasmas in nonhuman primates (nhp). the present study aimed to investigate the occurrence of and assess the phylogenetic position of bartonella spp. and hemoplasma species infecting neotropical nhp from brazilian amazon. from 2009 to 2013, a total of 98 blood samples from nhp belonging to the family cebidae were collected in the island of são luís, state of maranhão, of which 87 nhp were from wild animal screen ... | 2015 | 26577193 |
| identification of mycoplasma suis antigens and development of a multiplex microbead immunoassay. | the aims of the current study were to identify mycoplasma suis antigens and develop a multiplex microbead immunoassay (mia). a m. suis-expression library was screened for immunogens using sera from infected pigs. based on bioinformatics, putative antigens were identified within positive inserts; gene fragments were expressed and purified as polyhistidine fusion proteins, and immunoreactivity was confirmed by western blot. selected antigens were used to develop a mia. sera from noninfected and in ... | 2014 | 24557752 |
| identification of a novel hemoplasma species from pigs in zhejiang province, china. | hemoplasmas belong to mycoplasmataceae (mollicutes: mycoplasmatales) and are able to infect a broad range of mammalian species. we investigated prevalence of hemotropic mycoplasma species in pig farms in the region of zhejiang by a pcr scheme using universal primers targeting 16s rrna and rnase p rna gene (rnpb). representative positive samples from different farms were selected for sequencing of 16s rrna and the 219bp rnpb gene fragments for phylogenetic analysis. sequencing analysis of pcr pro ... | 2017 | 28381682 |
| high occurrence of mycoplasma suis infection in swine herds from non-technified farms in mossoró, state of rio grande do norte, northeastern brazil. | mycoplasma suis, the etiological agent of swine hemoplasmosis, has been neglected in swine herds around the world. swine hemoplasmosis is frequently associated with hemolytic anemia, disgalacty, infertility and immunosuppression, and it results in significant economic losses. this study investigates the occurrence of m. suis in non-technified swine herds in the northeastern region of brazil using quantitative pcr (qpcr) based on the 16s rrna gene. between march and august 2013, blood samples fro ... | 2016 | 27982298 |
| updating the proteome of the uncultivable hemotrophic mycoplasma suis in experimentally infected pigs. | mycoplasma suis belongs to the hemotrophic mycoplasmas that are associated with acute and chronic anemia in a wide range of livestock and wild animals. the inability to culture m. suis in vitro has hindered its characterization at the molecular level. since the publication of m. suis genome sequences in 2011 only one proteome study has been published. aim of the presented study was to significantly extend the proteome coverage of m. suis strain ki_3806 during acute infection by applying three di ... | 2016 | 26678042 |
| identification of mycoplasma suis msg1 interaction proteins on porcine erythrocytes. | adhesion protein msg1 mediating adherence to porcine erythrocytes in mycoplasma suis (m. suis) invasion has been identified previously. in order to determine the host membrane proteins that interact with msg1, recombinant his-tagged msg1 (rmsg1) was used to screen for interacting proteins in the protein extracts of porcine erythrocyte membrane. potential rmsg1-interacting proteins were initially identified as band 3 and β-actin with molecular weight of 46 and 45 kda, respectively. immune fluores ... | 2015 | 25344885 |
| pathobiology of mycoplasma suis. | mycoplasma suis is an uncultivable bacterium lacking a cell wall that attaches to and may invade the red blood cells of pigs. m. suis infections occur worldwide and cause the pig industry serious economic losses due to the disease known as infectious anaemia of pigs or, historically, porcine eperythrozoonosis. infectious anaemia of pigs is characterised predominantly by acute haemolytic or chronic anaemia, along with non-specific manifestations, such as growth retardation in feeder pigs and poor ... | 2014 | 25128978 |
| interactive host cells related to mycoplasma suis α-enolase by yeast two-hybrid analysis. | mycoplasma suis belongs to the haemotrophic mycoplasmas, which colonise the red blood cells of a wide range of vertebrates. adhesion to red blood cells is the crucial step in the unique lifecycle of m. suis. in addition to msg1 protein, α-enolase is the second adhesion protein of m. suis, and may be involved in the adhesion of m. suis to porcine red blood cells (rbc). to simulate the environment of the rbc, we established the cdna library of swine peripheral blood mononuclear cells (pbmc). the y ... | 2014 | 25085536 |
| seroprevalence and risk factors of mycoplasma suis infection in pig farms in central china. | mycoplasma suis, the causative agent of porcine infectious anemia, causes large economic losses to the swine industry worldwide. a questionnaire-based survey was conducted in 69 pig farms in hubei province, china, from november 2011 to august 2013 to ascertain the prevalence and associated risk factors of m. suis. four thousand and four blood samples from pigs of all the age groups were tested for m. suis antibodies using the established rmsg1-elisa assay. among these 4004 samples, 1615 blood sa ... | 2014 | 25081945 |
| quantitative pcr analysis of mycoplasma suis shedding patterns during experimental infection. | the uncultivable hemotrophic bacterium mycoplasma suis causes infectious anemia in pigs worldwide. the mechanisms by which m. suis is transmitted from pig to pig are largely unknown. thus, the present study aimed at investigating urine, feces, saliva, nasal and vaginal secrets as well as environmental samples for the presence of m. suis dna to get insights into potential transmission routes. seven pigs were experimentally infected with m. suis ki3806. samples were taken for 8 days post infection ... | 2014 | 25037994 |
| clinical and haematological characterisation of mycoplasma suis infections in splenectomised and non-splenectomised pigs. | mycoplasma suis causes infectious anaemia in pigs (iap), which can manifest in various degrees of severity depending on the virulence and the host's susceptibility. as m. suis cannot be cultured in vitro experimental infections of splenectomised animals play an essential role for pathogenesis research. the aim of the present study was to characterise the course of experimental infection using the highly virulent and red blood cell (rbc-) invasive m. suis strain ki3806, to compare the experimenta ... | 2014 | 24933162 |