cuticular hydrocarbons of the ectoparasitic wasp cephalonomia hyalinipennis (hymenoptera: bethylidae) and its alternative host, the stored product pest caulophilus oryzae (coleoptera: curculionidae).cuticular hydrocarbons of an ectoparasitic wasp attacking two beetle hosts have been identified and examined for the influence of age, gender, mating status, and host on hydrocarbon composition. the 37 wasp hydrocarbons identified consisted of a series of n-alkanes (c16 to c33), 3-, 5-, 9-, 10-, 11-, and 12-methyl alkanes and a series of z-7 and z-9 monoenes (c23:1 to c27:1). one c25:2 diene was found. no effects of hydrocarbon composition as a function of age, gender, or mating status were foun ...200212173292
bacillus thuringiensis serovar israelensis is highly toxic to the coffee berry borer, hypothenemus hampei ferr. (coleoptera: scolytidae).a native collection of bacillus thuringiensis strains was screened, once a reliable bioassay technique to assess the toxicity against the coffee berry borer (cbb) first-instar larvae was developed. a first round of bioassays with 170 strains indicated that the great majority of them showed no or very little insecticidal activity and that very few showed significant levels of toxicity. interestingly, only those strains that had previously been associated with mosquitocidal activity were also toxi ...200313129610
[occurrence of cephalonomia stephanoderis betrem (hymenoptera: bethylidae) in coffee plantations of the brazilian amazonia].adults of cephalonomia stephanoderis betrem were detected (in november 2003) during the sampling of coffea canephora pierre ex a. froehner berries damaged by hypothenemus hampei ferrari (coleoptera: scolytidae) in ouro preto d'oeste - rondônia, brazil (10 degrees 45's and 62 degrees 15'w). from january to july 2004, the parasitoid was monthly sampled in 200 berries damaged by h. hampei. probably, c. stephanoderis can already exert some parasitism pressure at the coffee berry borer population. th ...200617061809
effect of a bowman-birk proteinase inhibitor from phaseolus coccineus on hypothenemus hampei gut proteinases in vitro.the coffee berry borer, hypothenemus hampei (ferrari), is an important devastating coffee pest worldwide. both trypsin and chymotrypsin enzyme activities from h. hampei larval midgut can be inactivated by proteinaceous enzyme-inhibitors. a serine proteinase inhibitor belonging to the bowman-birk class was purified from a wild accession of phaseolus coccineus l. seeds. the inhibitor (pcbbi1) is a cysteine-rich protein that is heat-stable at alkaline ph. maldi-tof/ms analysis showed that pcbbi1 oc ...200718020416
alimentary canal and reproductive tract of hypothenemus hampei (ferrari) (coleoptera: curculionidae, scolytinae).the alimentary canal and the reproductive tract of males and females of hypothenemus hampei ferrari are described. the alimentary canal of h. hampei showed the crop with several spine-like structures and the midgut with few gastric caeca. we evidenced for the first time that adult females need to feed on coffee in order to produce viable eggs before and during oviposition period. the presence of air bubbles inside the anterior midgut may be due to starvation rather than the response of the envir ...200818506292
increased pathogenicity against coffee berry borer, hypothenemus hampei (coleoptera: curculionidae) by metarhizium anisopliae expressing the scorpion toxin (aait) berry borer (cbb) is the worlds most devastating coffee pest causing an estimated us$500 million worth of losses annually through damage and control costs. beauveria bassiana and metarhizium anisopliae have been employed to control this pest but their low virulence (slow kill and large inoculums) is an important factor constraining their use. m. anisopliae (aait-ma549) has been modified to express the scorpion toxin (aait) in insect hemolymph and this greatly increased pathogenicity again ...200818597773
first record of the coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: scolytidae), in pará nut, bertholletia excelsa (lecythidaceae).we report the occurrence of hypothenemus hampei (ferrari) attacking pará nuts stored in the southeast of para state. the coffee berry borer successfully colonized and reproduced using pará nuts as a food source. based on this observation, the pará nuts can be used as an alternative food source in rearing the coffee berry borer. also, attention should be brought to need of proper storage of these nuts to avoid infestation by this pest.200919618062
activity of bacillus thuringiensis hybrid protein against a lepidopteran and a coleopteran pest.the use of cry proteins from bacillus thuringiensis are an important strategy for biological control. recently it has been demonstrated that cry hybrid proteins (by domain swapping) resulted in improved toxicities in comparison with parental proteins. here, an sn1917 hybrid toxin was constructed and tested against colombian pest insects tecia solanivora (lepidoptera: gelechiidae), a severe potato pest, and hypothenemus hampei (coleoptera: scolytidae), which attacks coffee crops. the sn1917 proto ...201020002185
cloning and expression of an endo-1, 4-beta-xylanase from the coffee berry borer, hypothenemus hampei.abstract: background: the coffee berry borer, hypothenemus hampei, reproduces and feeds exclusively on the mature endosperm of the coffee seed, which has a cell wall composed mainly of a heterogeneous mixture of hemicellulose polysaccharides, including arabinoxylans. xylanases are digestive enzymes responsible for the degradation of xylan based polymers, hydrolyzing them into smaller molecules that are easier to assimilate by insects. we report the cloning, expression and enzymatic characteriza ...201222233686
[diversity of filamentous fungi associated with hypothenemus hampei (ferrari) (coleoptera: scolytidae) and its galleries in berries of coffea canephora (pierre)].field sampling was carried out in ouro preto d'oeste - rondônia (10 degrees 45's and 62 degrees 15'w) to evaluate the mycobiota associated with hypothenemus hampei ferrari [cuticle, mouth, prothorax (mycangia), gut and feces] and its galleries on berries of coffea canephora pierre. ten genera (201 isolates) were directly related with the insect while five genera (20 isolates) were related with galleries on berries. all the genera identified in the insects were also present in their galleries, wh ...200617144127
new record for the coffee berry borer, hypothenemus hampei, in hawaii.the coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae) is endemic to africa and is the most devastating pest of coffee worldwide. the female bores a hole in the coffee berry and deposits her eggs inside. upon hatching, larvae feed on the seeds, thus reducing both quality and yields of the marketable product. the coffee berry borer was found in the district of kona on the island of hawaii in august 2010 and appears to be restricted to that area.201122225430
draft genome of the most devastating insect pest of coffee worldwide: the coffee berry borer, hypothenemus hampei.the coffee berry borer, hypothenemus hampei, is the most economically important insect pest of coffee worldwide. we present an analysis of the draft genome of the coffee berry borer, the third genome for a coleopteran species. the genome size is ca. 163 mb with 19,222 predicted protein-coding genes. analysis was focused on genes involved in primary digestion as well as gene families involved in detoxification of plant defense molecules and insecticides, such as carboxylesterases, cytochrome p450 ...201526228545
genome sequence of the mosquitocidal bacillus thuringiensis strain br58, a biopesticide product effective against the coffee berry borer (hypothenemus hampei).bacillus thuringiensis is an important microbial control agent against insect pests. the draft genome sequence of the brazilian strain br58 described here contains the insecticidal genes cry4a, cry4b, cry10a, cry11a, cry60a, cry60b, and cyt1a, which show toxicity to both aedes aegypti and hypothenemus hampei larvae.201526659669
economic injury level for the coffee berry borer (coleoptera: curculionidae: scolytinae) using attractive traps in brazilian coffee fields.the currently existing sample procedures available for decision-making regarding the control of the coffee berry borer hypothenemus hampei (ferrari) (coleoptera: curculionidae: scolytinae) are time-consuming, expensive, and difficult to perform, compromising their adoption. in addition, the damage functions incorporated in such decision levels only consider the quantitative losses, while dismissing the qualitative losses. traps containing ethanol, methanol, and benzaldehyde may allow cheap and e ...201122299352
cascading indirect effects in a coffee agroecosystem: effects of parasitic phorid flies on ants and the coffee berry borer in a high-shade and low-shade habitat.nonconsumptive effects (nce) of parasites on hosts vary with habitat complexity thereby modifying trait-mediated effects on lower trophic levels. in coffee agroecosystems, pseudacteon sp. phorid fly parasites negatively affect azteca instabilis f. smith ants via nce thereby indirectly benefiting prey. it is unknown how differences in habitat complexity influence azteca-phorid interactions or how phorids affect the coffee berry borer (hypothenemus hampei ferrari), an important pest of coffee (cof ...201122251635
integrated pest management of coffee berry borer: strategies from latin america that could be useful for coffee farmers in hawaii.the coffee berry borer (cbb), hypothenemus hampei ferrari (coleoptera: curculionidae: scolytinae) is the primary arthropod pest of coffee plantations worldwide. since its detection in hawaii (september 2010), coffee growers are facing financial losses due to reduced quality of coffee yields. several control strategies that include cultural practices, biological control agents (parasitoids), chemical and microbial insecticides (entomopathogenic fungi), and a range of post-harvest sanitation pract ...201626848690
spore density and viability of entomopathogenic fungal isolates from indonesia, and their virulence against aphis gossypii glover (homoptera: aphididae).the focus of this study was on quantifying fitness attributes, such as spore density and viability, and determining the virulence level against aphid (aphis gossypii) nymphs of isolates from the fungal species beauveria bassiana and metarhizium anisopliae. the fungal isolates were obtained from several insects, including plutella xylostella, hypothenemus hampei, bronstispa longissima, a. gossypii, tenebrio molitor, and leptocorisa acuta, that were collected from indonesian islands, such as sumat ...201024575186
temperature-dependent development and emergence pattern of hypothenemus hampei (coleoptera: curculionidae: scolytinae) from coffee berries.the coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae: scolytinae), is the most important constrain for coffee production throughout the world. knowledge on the emergence pattern of h. hampei females to infest new berries is crucial to effectively plan control measures. in this laboratory study, we assessed the development of immature stages and the emergence pattern of h. hampei females from the berries by exposing them to temperatures that are typical for high-altitu ...201020857723
coffee berry borer (hypothenemus hampei)—a vector for toxigenic molds and ochratoxin a contamination in coffee berry borer (cbb, hypothenemus hampei ferrari) is a common insect pest in coffee plantations and is a suspected vector of various mycotoxin-producing molds. in the present study, field trials were undertaken consecutively for 3 years to evaluate the impact of cbb on the microbial contamination of arabica and robusta coffee bean varieties, with emphasis laid toward ochratoxin a (ota)-producing fungi. results revealed higher microbial contamination in cbb-infested beans in both the varietie ...201020618085
cry1b and cry3a are active against hypothenemus hampei ferrari (coleoptera: scolytidae).cry1b and cry3 proteins from bacillus thuringiensis are toxic to beetles such as the colorado potato beetle and the cottonwood leaf beetle. we report the development of a suitable rearing, bioassay method and the toxicity of these cry proteins to coffee berry borer first instar larvae.200919465024
natural occurrence of beauveria bassiana in hypothenemus hampei (coleoptera: curculionidae) populations in unsprayed coffee fields.three unsprayed coffee farms (farm 1, 2 and 3) were studied for the natural occurrence of the insect pathogenic fungus beauveria bassiana in hypothenemus hampei populations throughout the rainy season of 2004 (july-november) and 2005 (july-december). b. bassiana infections were found during most sampling dates in both years, on all three farms. the b. bassiana infection levels were higher in 2005 than in 2004 with mean prevalence of 12.1% and 2.7%, respectively. no consistent significant differe ...200817854825
cloning and biochemical characterization of an endo-1,4-β-mannanase from the coffee berry borer hypothenemus hampei.the study of coffee polysaccharides-degrading enzymes from the coffee berry borer hypothenemus hampei, has become an important alternative in the identification for enzymatic inhibitors that can be used as an alternative control of this dangerous insect. we report the cloning, expression and biochemical characterization of a mannanase gene that was identified in the midgut of the coffee berry borer and is responsible for the degradation of the most abundant polysaccharide in the coffee bean.201323965285
an alpha-amylase inhibitor gene from phaseolus coccineus encodes a protein with potential for control of coffee berry borer (hypothenemus hampei).plant alpha-amylase inhibitors are proteins found in several plants, and play a key role in natural defenses. in this study, a gene encoding an alpha-amylase inhibitor, named alphaai-pc1, was isolated from cotyledons of phaseolus coccineus. this inhibitor has an enhanced primary structure to p. vulgaris alpha-amylase inhibitors (alpha-ai1 and alpha-ai2). the alphaai-pc1 gene, constructed with the pha-l phytohemaglutinin promoter, was introduced into tobacco plants, with its expression in regener ...200616901522
phylogenetic origins of african and neotropical beauveria bassiana s.l. pathogens of the coffee berry borer, hypothenemus hampei.a phylogenetic epidemiological study of beauveria bassiana s.l. was conducted for african and neotropical pathogens of the coffee berry borer (cbb), hypothenemus hampei, based on inferences from two nuclear intergenic regions, efutr and bloc. cbb pathogens were distributed among four terminal clades, however, the majority of african and neotropical isolates cluster in a well-supported monophyletic group, informally designated afneo_1. although the relationship between african and neotropical afn ...200616806258
farmers' perceptions, knowledge, and management of coffee pests and diseases and their natural enemies in chiapas, mexico.small farmers' perceptions of coffee coffea arabica l. herbivores and their natural enemies, how those perceptions relate to field infestation levels, and pest management practices being implemented by members from two organic and nonorganic coffee grower organizations in the soconusco region, southeastern mexico, were analyzed through an interview survey, diagnostic workshops, and field sampling. the terms pest, disease, and damage were commonly used as synonyms. the major phytophagous species, ...200415568334
metaparasitylenchus hypothenemi n. sp. (nematoda: allantonematidae), a parasite of the coffee berry borer, hypothenemus hampei (curculionidae: scolytinae).metaparasitylenchus hypothenemi n. sp. (nematoda: allantonematidae) is described from the coffee berry borer, hypothenemus hampei (ferrari) (curculionidae: scolytinae), in chiapas, mexico. this species differs from other members of the genus by its small size, annulated cuticle, lateral fields with 3 ridges, free-living stages with an excretory pore located between the pharyngeal gland orifices, a distinct stylet with basal swellings in free-living females, a postvulval uterine extension, a thin ...200415562611
volatile composition of coffee berries at different stages of ripeness and their possible attraction to the coffee berry borer hypothenemus hampei (coleoptera: curculionidae).the analysis of volatile emissions of coffee berries in different physiological states of ripeness was performed using dynamic headspace and gas chromatography/mass spectrometry analysis for coffea arabica, var. colombia. the composition of the volatiles emitted by coffee berries is dominated by very high levels of alcohols, mainly ethanol, in all stages of ripeness in comparison with other compounds. overripe coffee berries have high volatile emissions and show a composition dominated mainly by ...200415366842
effect of beauveria bassiana and metarhizium anisopliae (deuteromycetes) upon the coffee berry borer (coleoptera: scolytidae) under field conditions.the effect of three strains of the fungus beauveria bassiana (balsamo) vuillemin and two strains of metarhizium anisopliae (metschnikoff) sorokin upon the coffee berry borer, hypothenemus hampei (ferrari), was studied in three coffee farms at different altitudes (450-1,100 m above sea level) in soconusco, chiapas, mexico. the maximum average percentage mycosis varied according to altitude. at 450 m asl (el rincon) mycosis was 14.3% for b. bassiana and 6.3% for m. anisopliae; at 880 m asl (santa ...200011057711
lack of nucleotide variability in a beetle pest with extreme inbreeding.the coffee berry borer beetle hypothenemus hampei (ferrari) (curculionidae: scolytinae) is the major insect pest of coffee and has spread to most of the coffee-growing countries of the world. this beetle also displays an unusual life cycle, with regular sibling mating. this regular inbreeding and the population bottlenecks occurring on colonization of new regions should lead to low levels of genetic diversity. we were therefore interested in determining the level of nucleotide variation in nucle ...19989535165
evaluation of the fungus beauveria bassiana as a potential biological control agent against phlebotomine sand flies in colombian coffee colombia, the entomopathogenic fungus beauveria bassiana (deuteromycotina: hyphomycetes) is widely used to control the coffee berry borer hypothenemus hampei (coleoptera: scolytidae) in coffee plantations. recent studies suggested that this fungus is also pathogenic to several important vectors of disease, including phlebotomus papatasi and lutzomyia longipalpis (diptera: psychodidae). the present study evaluated the use of b. bassiana as a potential biological control agent against phlebotom ...19979281401
increasing coffee berry borer (coleoptera: curculionidae: scolytinae) female density in artificial diet decreases fecundity.three experiments were conducted to determine the influence of number of coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae: scolytinae), females (one, two, or five) reared in artificial diet on fecundity and subsequent development of larvae, pupae, and adults. our results demonstrated that increasing female density from one to two or five individuals did not result in the expected two- or five-fold increase in progeny, despite ample food resources available. instead, d ...201121404844
[susceptibility of the parasitoid phymastichus coffea lasalle (hymenoptera: eulophidae) to beauveria bassiana under laboratory conditions].the coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae), is the most important coffee pest worldwide. beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and phymastichus coffea lasalle (hymenoptera: eulophidae) is an african endoparasitoid of h. hampei adults, recently imported to several latin american and caribbean countries to aid in the coffee berry borer control. the objective of this study was to determine ...200919943017
[temporal and spatial distribution of ants in a light gradient, in a coffee agroforestry system, turrialba, costa rica].shade trees are frequently present in coffee (coffea arabica l.) agroforestry systems of mesoamerica. these systems can harbor a rich entomofauna, including ants, which could be predators of key pests in these systems. however, the role of shade on the distribution and abundance of these ants is unknown, yet such knowledge could suggest guidelines for manipulating certain environmental conditions of their habitat, thereby achieving their conservation and increase. therefore, we studied the effec ...200719086397
activity of alpha-amylase inhibitors from phaseolus coccineus on digestive alpha-amylases of the coffee berry borer.seeds of scarlet runner bean ( phaseolus coccineus l.) were analyzed for alpha-amylase inhibitor (alpha-ai) activity. through the use of polyclonal antibodies raised against pure alpha-ai-1 from common bean ( phaseolus vulgaris l.), typical alpha-alphaiota polypeptides (mr 14-18 kda) as well as a large polypeptide of mr 32000 da, usually referred to as "amylase inhibitor like", were detected. the inhibitor activity present in four accessions of p. coccineus was examined, both in semiquantitative ...200818321052
diversity of bacillus thuringiensis strains isolated from coffee plantations infested with the coffee berry borer hypothenemus hampei.the coffee berry borer hypothenemus hampei ferrari (coleoptera: scolytidae) was first reported infecting costa rican coffee plantations in the year 2000. due to the impact that this plague has in the economy of the country, we were interested in seeking new alternatives for the biological control of h. hampei, based on the entomopathogenic bacteria bacillus thuringiensis. a total of 202 b. thuringiensis isolates obtained from costa rican coffee plantations infested with h. hampei were analyzed t ...200417361568
coffee berry borer hypothenemus hampei (coleoptera: curculionidae): searching for sustainable control strategies.the coffee berry borer hypothenemus hampei (ferrari) is the most serious pest of the world's most valuable tropical export crop. since the last review on this insect was published six years ago, many new studies have contributed to an improved insight into the biology and ecology of the beetle, and have indicated new avenues for integrated and biological control. the latest developments in research, both laboratory and field, on the pest, its natural enemies and their implications for integrated ...200616768810
exploiting the genetic diversity of beauveria bassiana for improving the biological control of the coffee berry borer through the use of strain mixtures.beauveria bassiana is an entomopathogen widely used to control the coffee berry borer in colombia, as part of an integrated pest management strategy. traditionally, the development of fungal insect pathogens as biocontrol agents in crop pests has been oriented towards the selection and formulation of elite clonal strains. instead, we explored the potential application of genetic diversity in b. bassiana by determining the effect of strain mixtures on coffee berry borer mortality compared to clon ...200616362818
biological control of the coffee berry borer hypothenemus hampei (coleoptera: curculionidae) by phymastichus coffea (hymenoptera: eulophidae) in colombia.the potential of the eulophid parasitoid phymastichus coffea lasalle to control coffee berry borer hypothenemus hampei (ferrari) populations under field conditions in colombia was evaluated. parasitoid adults were released one, five and nine days after artificial infestations of 90-, 150- and 210-day-old coffee berries with h. hampei females. the position of the beetle inside the berry and the parasitism levels were assessed ten days after each p. coffea release. parasitism of h. hampei by p. co ...200516197567
mycobiota associated with the coffee berry borer (hypothenemus hampei) in mexico.field surveys were carried out in coffee plantations in chiapas, mexico, to collect and identify fungi associated with the cuticle, gut, faeces and galleries of the coffee berry borer, hypothenemus hampei. insects and coffee berries containing galleries were collected in three coffee farms at different altitudes: rosario izapa (425 m), la alianza (700 m) and monteperla (950 m). an additional sample consisting of coffee berry borers reared in the laboratory on meridic diets was also included. res ...200312967216
assessment of insecticide resistance in five insect pests attacking field and vegetable crops in nicaragua.field populations of hypothenemus hampei (ferrari), plutella xylostella (l.), spodoptera exigua (hübner), helicoverpa zea (boddie) and bemisia tabaci (gennadius) were tested for resistance to several insecticides commonly used in nicariagua. assays were conducted to estimate the ld50s or lc50s and the corresponding resistance ratios. a diagnostic concentration was used to discriminate between susceptible and resistant strains of h. hampei. the tests with >6,000 h. hampei adults collected from si ...200011142313
transcriptome analysis of the entomopathogenic fungus beauveria bassiana grown on cuticular extracts of the coffee berry borer (hypothenemus hampei).the coffee berry borer (cbb; hypothenemus hampei) is a major pest of coffee responsible for significant crop losses worldwide. the entomopathogenic fungus beauveria bassiana represents a natural means of controlling this insect pest; however, little is known concerning the molecular determinants that contribute to the virulence of the fungus towards the cbb. in order to examine genes involved in insect virulence, two expressed sequence tag (est) libraries, representing germinating conidia and gr ...201222461485
cuticle-degrading proteases produced by the entomopathogenic fungus beauveria bassiana in the presence of coffee berry borer cuticle.a brazilian isolate of beauveria bassiana (cg425) that shows high virulence against the coffee berry borer (cbb) was examined for the production of subtilisin-like (pr1) and trypsin-like (pr2) cuticle-degrading proteases. fungal growth was either in nitrate-medium or in cbb cuticle-containing medium under both buffered and unbuffered conditions. in unbuffered medium supplemented with cuticle, the ph of cultures dropped and pr1 and pr2 activities were detected in high amounts only at a ph of 5.5 ...200824031220
genome-wide analysis of transposable elements in the coffee berry borer hypothenemus hampei (coleoptera: curculionidae): description of novel families.the coffee berry borer (cbb) hypothenemus hampei is the most limiting pest of coffee production worldwide. the cbb genome has been recently sequenced; however, information regarding the presence and characteristics of transposable elements (tes) was not provided. using systematic searching strategies based on both de novo and homology-based approaches, we present a library of tes from the draft genome of cbb sequenced by the colombian coffee growers federation. the library consists of 880 sequen ...201728204924
landscape context and scale differentially impact coffee leaf rust, coffee berry borer, and coffee root-knot nematodes.crop pest and disease incidences at plot scale vary as a result of landscape effects. two main effects can be distinguished. first, landscape context provides habitats of variable quality for pests, pathogens, and beneficial and vector organisms. second, the movements of these organisms are dependent on the connectivity status of the landscape. most of the studies focus on indirect effects of landscape context on pest abundance through their predators and parasitoids, and only a few on direct ef ...201222611856
effects of the aspartic protease inhibitor from lupinus bogotensis seeds on the growth and development of hypothenemus hampei: an inhibitor showing high homology with storage proteins.the coffee berry borer hypothenemus hampei is a pest that causes great economic damage to coffee grains worldwide. because the proteins consumed are digested by aspartic proteases in the insect's midgut, the inhibition of these proteases by transferring a gene encoding an aspartic protease inhibitor from lupinus bogotensis benth. to coffee plants could provide a promising strategy to control this pest. five aspartic protease inhibitors from l. bogotensis (lbapi) were accordingly purified and cha ...201424314849
seed-specific stable expression of the α-ai1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer.genetic transformation of coffee (coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. the transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (pha-l) from phaseolus vulgaris were characterized in genetically modified c. arabica expressing the α-amylase inhibitor-1 (α-ai1) gene. the α-ai1 inhibitor shows con ...201726697127
can coffee chemical compounds and insecticidal plants be harnessed for control of major coffee pests?pests and pathogens threaten coffee production worldwide and are difficult to control using conventional methods, such as insecticides. we review the literature on the chemistry of coffee, concentrating on compounds most commonly reported from coffea arabica and coffea canephora. differences in chemistry can distinguish coffee species and varieties, and plants grown under different biogeographic conditions exhibit different chemotypes. a number of chemical groups, such as alkaloids and caffeoylq ...201526458882
molecular markers as a method to evaluate the movement of hypothenemus hampei (ferrari).the objective of this research was to develop a methodology to describe the movement of the coffee berry borer hypothenemus hampei (coleoptera: curculionidae) in the field through: (i) the evaluation of allele variation of a microsatellite marker on polymorphic colombian h. hampei populations; (ii) the invention of a device for releasing h. hampei adults; (iii) the standardization of a release-recapture technique for h. hampei populations; (iv) the estimation of the flight distance of the insect ...201526078300
molecular markers detect cryptic predation on coffee berry borer (coleoptera: curculionidae) by silvanid and laemophloeid flat bark beetles (coleoptera: silvanidae, laemophloeidae) in coffee beans.the coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae), is a serious pest of coffee worldwide. it was first detected in hawai'i in 2010. two predatory beetles, cathartus quadricollis (coleoptera: silvanidae) and leptophloeus sp. (coleoptera: laemophloeidae), have been observed in h. hampei-infested coffee. under laboratory conditions, colony-reared c. quadricollis and leptophloeus sp. prey upon all life stages of h. hampei. however, the h. hampei life cycle occurs almo ...201626487745
caffeine and resistance of coffee to the berry borer hypothenemus hampei (coleoptera: scolytidae).the role of caffeine as a chemical defense of coffee against the berry borer hypothenemus hampei was investigated. no positive correlation was observed between resistance and caffeine content in experiments in which seeds from several coffee species presenting genetic variability for the alkaloid were exposed to adult insects. the same was observed in an experiment with coffee seeds that had their caffeine content doubled by imbibition with caffeine aqueous solutions. other experiments showed th ...200314611159
[reduced survival and infestation of coffee borer, hypothenemus hampei (ferrari) (coleoptera: scolytidae), on coffee fruits, in response to neem sprays in laboratory].aqueous solutions of neem oil and aqueous extracts of neem seeds and leaves were sprayed on coffee fruits for laboratory evaluation of their efficiency in reducing infestation of the coffee borer, hypothenemus hampei (ferrari), in multi-choice preference assays in laboratory. neem oil and extracts reduced infestation of fruits in a dose-dependent manner, acting as a repellent. at 0.5%, 1% and 1.5%, the oil reduced fruit infestation by 30.2%, 42.5% (p > 0.05), and 58.6% (p < 0.05), respectively, ...201620878003
some like it hot: the influence and implications of climate change on coffee berry borer (hypothenemus hampei) and coffee production in east africa.the negative effects of climate change are already evident for many of the 25 million coffee farmers across the tropics and the 90 billion dollar (us) coffee industry. the coffee berry borer (hypothenemus hampei), the most important pest of coffee worldwide, has already benefited from the temperature rise in east africa: increased damage to coffee crops and expansion in its distribution range have been reported. in order to anticipate threats and prioritize management actions for h. hampei we pr ...201121935419
an inhibitor from lupinus bogotensis seeds effective against aspartic proteases from hypothenemus hampei.the coffee berry borer, hypothenemus hampei (ferrari), is one of the most devastating coffee pests (coffea arabica l.) worldwide. digestion in the midgut of h. hampei is facilitated by aspartic proteases. this is the first report of an aspartic protease inhibitor from lupinus bogotensis. the l. bogotensis aspartic protease inhibitor (lbapi) exhibited a molecular mass of 12.84kda, as determined by maldi-tof, and consists of a single polypeptide chain with an isoelectric point of 4.5. in thermal a ...201020347105
semiochemicals used in host location by the coffee berry borer, hypothenemus hampei.the coffee berry borer, hypothenemus hampei is a serious pest in many coffee growing countries. electrophysiological and behavioral responses of h. hampei to volatiles of different phenological stages of coffee, coffea arabica, fruits were studied in order to identify volatile semiochemicals used in host location. volatiles were collected from different phenological stages of c. arabica fruit by air entrainment. electrophysiological recordings were made from insect antennae. behavioral assays we ...200919714284
["conilon" coffee berries bored by hypothenemus hampei (ferrari) (coleoptera: scolytidae): what matters if they drop down during the fruiting phase?].falling of berries bored by hypothenemus hampei (ferrari) may be the major loosing factor during the fruiting period. however, only those bored berries which remain in the soil surface before a new yielding period have been recognized as responsible for the damage level ho achieved by new developing berries. in this paper, we investigated in the plants and in the soil surface, the presence of coffea canephora cv. conilon berries bored by h. hampei during the yielding period in ouro preto d'oeste ...200818575701
insecticidal activity of caffeine aqueous solutions and caffeine oleate emulsions against drosophila melanogaster and hypothenemus hampei.the bioactivity of caffeine aqueous solutions (0.20-2.00 wt %) and caffeine oleate emulsions (20 vol % oil, 2.00 wt % surfactant, 0.04 wt % caffeine, 0.05 wt % oleic acid) was assessed against two biological models: drosophila melanogaster and hypothenemus hampei. the caffeine aqueous solutions showed no insecticidal activity, whereas caffeine oleate emulsions had high bioactivity against both d. melanogaster and h. hampei. by preparing the caffeine oleate emulsions with anionic surfactants (i.e ...200717658827
single-stranded dna conformation polymorphism at the rdl locus in hypothenemus hampei (coleoptera: scolytidae).the homologue of the resistance to dieldrin gene (rdl) in drosophila melanogaster was cloned and sequenced in the scolytid beetle hypothenemus hampei, a coffee pest resistant to cyclodiene insecticides in new caledonia. the amino acid sequence of the rdl exon no. 7 protein product in h. hampei was identical to that in d. melanogaster and showed the same amino acid change as that characterizing susceptible vs. resistant d. melanogaster. samples from natural h. hampei populations (from asia, the p ...19968617613
in vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae) and the fungus hemileia vastatrix the most limiting pests of coffee crops.two genes from streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in escherichia coli in form of a fusion protein with a maltose binding protein (mbp). the goal was to produce and test proteins that inhibit both the coffee berry borer insect hypothenemus hampei and the coffee rust fungus hemileia vastatrix. both recombinant proteins mbp/exochitinase and mbp/endochitinase showed chitinolytic activity. when recombinant purified proteins ...201222464210
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