| proposal of six new species in the genus aureobacterium and transfer of flavobacterium esteraromaticum omelianski to the genus aureobacterium as aureobacterium esteraromaticum comb. nov. | twelve strains placed in the genera flavobacterium, pseudomonas, and aureobacterium, including soil isolates, were characterized taxonomically. on the basis of morphological, physiological, and chemotaxonomic data, as well as dna-dna hybridization data, we propose that 11 of these strains should be classified in the genus aureobacterium as new combinations or new species, as follows: aureobacterium esteraromaticum comb. nov. (type strain, ifo 3751 [= atcc 8091]), aureobacterium arabinogalactanol ... | 1993 | 8347513 |
| purification and characterization of a prolidase from aureobacterium esteraromaticum. | an edta-insensitive prolidase (proline dipeptidase, ec 3.4.13.9) was isolated from a cell-free extract of aureobacterium esteraromaticum ifo 3752. the enzyme was purified almost to homogeneity using acetone precipitation, hydrophobic chromatography, ion-exchange chromatography, and gel-permeation chromatography. the enzyme has a molecular weight of about 440,000 by gel permeation chromatography, and about 40,000 by sds polyacrylamide gel electrophoresis. the isoelectric point was 4.6. the enzyme ... | 1996 | 8782407 |
| union of the genera microbacterium orla-jensen and aureobacterium collins et al. in a redefined genus microbacterium. | the 16s rrna gene sequences of 19 strains, 11 strains representing validated aureobacterium or microbacterium species and eight strains of non-valid species or isolates, were determined. these sequences were aligned with the sequences of other validated aureobacterium and microbacterium species and related actinobacteria. a comparative sequence analysis of 43 strains revealed that the species of the genera aureobacterium and microbacterium form a monophyletic association in which species of both ... | 1998 | 9734028 |
| cloning of a novel prolidase gene from aureobacterium esteraromaticum. | the prolidase gene from aureobacterium esteraromaticum was cloned and expressed in escherichia coli. the cloned enzyme had the same enzymatic properties as the wild-type enzyme. kinetic analysis of the enzyme indicated that the best substrate was pro-hyp, which was not hydrolyzed by other prolidases. interestingly, there was no homology between the deduced amino acid sequence of a. esteraromaticum prolidase and those of the other sources such as human e. coli and lactobacillus. however, homology ... | 1999 | 9989239 |
| microbacterium aerolatum sp. nov., isolated from the air in the 'virgilkapelle' in vienna. | three rod-shaped, gram-positive strains were isolated from the air of the chapel 'virgilkapelle' in vienna. a representative of these three strains, strain v-73t, shared the highest 16s rdna sequence similarities with members of the genus microbacterium, in particular microbacterium foliorum, microbacterium testaceum, microbacterium esteraromaticum, microbacterium keratanolyticum and microbacterium arabinogalactanolyticum. the strains displayed almost identical biochemical and physiological char ... | 2002 | 12148633 |
| initial characterization of new bacteria degrading high-molecular weight polycyclic aromatic hydrocarbons isolated from a 2-year enrichment in a two-liquid-phase culture system. | to characterize some polycyclic aromatic hydrocarbons (pah)-degrading microorganisms isolated from an enriched consortium degrading high molecular weight (hmw) pahs in a two-liquid-phase (tlp) soil slurry bioreactor, and to determine the effect of low molecular weight (lmw) pah on their growth and hmw pah-degrading activity. | 2003 | 12534823 |
| pair-dependent co-aggregation behavior of non-flocculating sludge bacteria. | two strains of non-flocculating sewage sludge bacteria (xanthomonas sp. s53 and microbacterium esteraromaticum s51) showed 91% and 77% co-aggregation, respectively, with acinetobacter johnsonii s35 using a spectrophometric assay. the co-aggregates in case of xanthomonas sp. s53 and a. johnsonii s35 were above 100 microm and stable against edta (2 mm) and a commercial protease (0.2 mg ml(-1)). protease/periodate pretreatment of the partners did not affect this co-aggregation. on the other hand, c ... | 2003 | 12889835 |
| coaggregation among nonflocculating bacteria isolated from activated sludge. | thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. among these strains, eight showed significant (74 to 99%) coaggregation with acinetobacter johnsonii s35 while only four strains coaggregated, to a lesser extent (43 to 65%), with acinetobacter junii s33. the extent and pattern of coaggregation as well as the aggregate size showed good correlation w ... | 2003 | 14532062 |
| effects of ph amendment on metal working fluid wastewater biological treatment using a defined bacterial consortium. | the aim of this study was to determine whether ph amendment of a highly alkaline metal working fluid (mwf) wastewater would improve biological treatment in a bioreactor system following introduction of a bacterial inoculum (comprised of the following strains: agrobacterium radiobacter, comamonas testosteroni, methylobacterium mesophilicum, microbacterium esteraromaticum, and microbacterium saperdae). the ph values tested were 6, 7, 8, and 9. three replicate batch mode bioreactors inoculated with ... | 2005 | 15625673 |
| coaggregation between acinetobacter johnsonii s35 and microbacterium esteraromaticum strains isolated from sewage activated sludge. | the extent and nature of intergeneric coaggregations among non-flocculating sludge bacteria were studied through examination of the coaggregation abilities of acinetobacter johnsonii s35 with two other strains of non-flocculating sludge bacteria (microbacterium esteraromaticum s38 and m. esteraromaticum s51). at first, the effect of electrolyte concentration as well as the addition of edta and proteases on coaggregation were studied. changes in electrolyte concentration had little effect on the ... | 2003 | 16233476 |
| isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the southwest indian ocean ridge. | microorganisms play an important role in the geobiocycling of arsenic element. however, little is known about the bacteria involved in this process in oceanic environments. in this report, arsenite-resistant bacteria were detected in deep-sea sediments on the southwest indian ridge. from arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2-80 mm. phylogenetic analysis based on 16s rrna showed that they mainly belonged to proteobacteria and actinob ... | 2009 | 18841325 |
| isolation and characterization of biosurfactant-producing alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis. | biosurfactant-producing bacteria belonging to the genera alcanivorax, cobetia and halomonas were isolated from marine sediments with a history of hydrocarbon exposure (aristizábal and gravina peninsulas, argentina). two alcanivorax isolates were found to form naturally occurring consortia with strains closely related to pseudomonas putida and microbacterium esteraromaticum. alkane hydroxylase gene analysis in these two alcanivorax strains resulted in the identification of two novel alkb genes, s ... | 2009 | 18983915 |
| hydrolysis of fenamiphos and its toxic oxidation products by microbacterium sp. in pure culture and groundwater. | a bacterium with an exceptional ability to hydrolyse fenamiphos and its toxic oxidation products fenamiphos sulfoxide and fenamiphos sulfone, all possessing poc bond was isolated from soil. based on 16s rrna gene determination, this bacterium was putatively identified as microbacterium esteraromaticum. the phenols (fenamiphos phenol, sulfoxide phenol and sulfone phenol) formed during bacterial hydrolysis resisted further degradation in mineral salts medium and sterile groundwater, but were trans ... | 2009 | 19195880 |
| microbacterium soli sp. nov., an alpha-glucosidase-producing bacterium isolated from soil of a ginseng field. | five gram-type-positive, aerobic, rod-shaped, non-motile strains of microbacterium (dcy 17(t), ms1, ms2, ms3 and ms4) were isolated from soil from a ginseng field in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, these strains were shown to be related to microbacterium esteraromaticum dsm 8609(t) (96.1 %), m. xylanilyticum dsm 16914(t) (96.0 %), m. aquimaris js54-2(t) (95.6 %), m. insulae ds-66(t) (95.5 %), m. ketosireducens ifo 14548(t) (95.5 %) and m. arabinogalactano ... | 2010 | 19654351 |
| Microbacterium suwonense sp. nov., isolated from cow dung. | An actinomycete strain, designated M1T8B9(T), was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17(T) (98.2%) and Microbacterium esteraromaticum DSM 8609(T) (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosp ... | 2011 | 22068506 |
| enzymatic biotransformation of ginsenoside rb1 to 20(s)-rg3 by recombinant β-glucosidase from microbacterium esteraromaticum. | microbacterium esteraromaticum was isolated from ginseng field. the β-glucosidase gene (bgp1) from m. esteraromaticum was cloned and expressed in escherichia coli bl21 (de3). the bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. the recombinant β-glucosidase enzyme (bgp1) was purified and characterized. the molecular mass of purified bgp1 was 87.5 kda, as determined by sds-page. using 0.1 mg ml(-1) enzyme in 20 mm sodiu ... | 2012 | 22249721 |
| carbazole degradation in the soil microcosm by tropical bacterial strains. | in a previous study, three bacterial strains isolated from tropical hydrocarbon-contaminated soils and phylogenetically identified as achromobacter sp. strain sl1, pseudomonas sp. strain sl4 and microbacterium esteraromaticum strain sl6 displayed angular dioxygenation and mineralization of carbazole in batch cultures. in this study, the ability of these isolates to survive and enhance carbazole degradation in soil were tested in field-moist microcosms. strain sl4 had the highest survival rate (1 ... | 2017 | 26691461 |
| carbazole angular dioxygenation and mineralization by bacteria isolated from hydrocarbon-contaminated tropical african soil. | four bacterial strains isolated from hydrocarbon-contaminated soils in lagos, nigeria, displayed extensive degradation abilities on carbazole, an n-heterocyclic aromatic hydrocarbon. physicochemical analyses of the sampling sites (acpp, mwo, nesu) indicate gross pollution of the soils with a high hydrocarbon content (157,067.9 mg/kg) and presence of heavy metals. phylogenetic analysis of the four strains indicated that they were identified as achromobacter sp. strain sl1, pseudomonas sp. strain ... | 2014 | 24728574 |
| isolation and characterization of novel ginsenoside-hydrolyzing glycosidase from microbacterium esteraromaticum that transforms ginsenoside rb2 to rare ginsenoside 20(s)-rg3. | ginsenoside rb2 was transformed by recombinant glycosidase (bgp2) into ginsenosides rd and 20(s)-rg3. the bgp2 gene consists of 2,430 bp that encode 809 amino acids, and this gene has homology to the glycosyl hydrolase family 2 protein domain. sds-page was used to determine that the molecular mass of purified bgp2 was 87 kda. using 0.1 mg ml(-1) of enzyme in 20 mm sodium phosphate buffer at 40 °c and ph 7.0, 1.0 mg ml(-1) ginsenoside rb2 was transformed into 0.47 mg ml(-1) ginsenoside 20(s)-rg3 ... | 2013 | 23670791 |
| enzymatic transformation of the major ginsenoside rb2 to minor compound y and compound k by a ginsenoside-hydrolyzing β-glycosidase from microbacterium esteraromaticum. | the ginsenoside-hydrolyzing β-glycosidase (bgp3) derived from microbacterium esteraromaticum transformed the major ginsenoside rb2 to more pharmacologically active minor ginsenosides including compounds y and k. the bgp3 gene consists of 2,271 bp encoding 756 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. bgp3 is capable of hydrolyzing beta-glucose links and arabinose links. hplc analysis of the time course of ginsenoside rb2 hydrolysis by bgp3 (0.1 mg enzyme ... | 2012 | 22717707 |
| enzymatic biotransformation of ginsenoside rb1 to compound k by recombinant β-glucosidase from microbacterium esteraromaticum. | we cloned and characterized a β-glucosidase (bgp3) gene from microbacterium esteraromaticum isolated from ginseng field. the bgp3 gene consists of 2,271 bp encoding 756 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. the molecular mass of purified bgp3 was 80 kda, as determined by sds-page. the enzyme (bgp3) catalyzed the conversion of ginsenoside rb1 to the more pharmacologically active minor ginsenoside rd and compound k. the bgp3 hydrolyzed the outer glucose ... | 2012 | 22428991 |