| structural determinants of plant lignans for the formation of enterolactone in vivo. | the quantity of mammalian lignans enterolactone (enl) and enterodiol (end) and of plant lignans secoisolariciresinol (seco) and 7-hydroxymatairesinol (hmr) excreted in a 24-h rat urine sample was measured after a single p.o. dose of an equivalent quantity of secoisolariciresinol diglycoside (sdg), secoisolariciresinol (seco), matairesinol (mr), 7-hydroxymatairesinol (hmr) and enl. plant lignans (seco and hmr) were partially absorbed as such. the aglycone form of seco was more efficiently convert ... | 2002 | 12270222 |
| nutritional ecology of the formosan subterranean termite (isoptera: rhinotermitidae): feeding response to commercial wood species. | the feeding preferences of the formosan subterranean termite, coptotermes formosanus shiraki, were tested in three separate experiments on 28 different wood species. experiment 1 was a multiple-choice test designed to test relative preferences among 24 wood species commercially available in new orleans, la. experiment 2 was a similar study designed to test relative preferences among 21 wood species shown or reported to be unpalatable to the formosan subterranean termite. experiment 3 was a no-ch ... | 2001 | 11332848 |
| phytochemical evidence for the plant origin of brazilian propolis from são paulo state. | propolis and plant secretions from three species, most frequently mentioned as botanical sources of the bee glue in brazil (baccharis dracunculifolia, araucaria angustifolia and eucalyptus citriodora) have been investigated using gc-ms. based on chemical evidence, b. dracunculifolia was shown to be the main propolis source in são paulo state. the antibacterial and antifungal activities of all four materials were also tested, the most active being propolis and baccharis leaf exudate. | 1999 | 10431391 |
| evaluation of propolis. i. evaluation of brazilian and chinese propolis by enzymatic and physico-chemical methods. | to establish a method of evaluating propolis, the effects of the ethanol and water extracts from various collecting of propolis from different countries and plant sources on hyaluronidase activity were investigated along with their absorption spectra and specific absorbance (e(1%)1 cm value). the relations between the hyaluronidase inhibitory activities of these extracts and their e(1%)1 cm values were also examined, and the following was found: 1) the enzyme inhibitory activities of the ethanol ... | 1997 | 9178928 |
| arbuscular mycorrhizal fungi associated with araucaria angustifolia (bert.) o. ktze. | specimens of araucaria angustifolia from a native forest reserve and a reforested area in the state park of campos do jordão were studied to determine the number and diversity of spore populations of arbuscular mycorrhizal fungi (amf) and root colonization. six randomly chosen plots (planted with 8- to 12-year-old plants) were delimited, four in the native forest and two in the reforested area. rhizosphere and root samples were collected during two periods of the year corresponding to the rainy ... | 2003 | 12938034 |
| modulation of acute inflammation by a chitin-binding lectin from araucaria angustifolia seeds via mast cells. | the effects of a lectin (aal) from seeds of araucaria angustifolia were investigated in the model of rat paw edema. in vivo anti-and pro-inflammatory activities, role of sugar residues, inflammatory mediators and systemic toxicity were assessed. intravenous injection of aal (0.1-1 mg/kg) dose-dependently inhibited the dextran-induced increase in edema and vascular permeability, which were prevented by association of the lectin with its binding sugar n-acetyl-glucosamine (glyc-nac). aal also sign ... | 2006 | 16957941 |
| current state and projection of the probable original vegetation of the são carlos region of são paulo state, brazil. | a map of the native vegetation remaining in são carlos county was built based on aerial images, satellite images, and field observations, and a projection of the probable original vegetation was made by checking it against soil and relief surveys. the existing vegetation is very fragmented and improverished, consisting predominantly of cerrados (savanna vegetation of various physiognomies), semideciduous and riparian forest, and regeneration areas. araucaria angustifolia (bertol.) kuntze, found ... | 2003 | 14758712 |
| [larvae culture of ovine gastrintestinal nematodes in sawdust substrates of pinus taeda, p. elliottii and araucaria angustifolia]. | the aim of this study was to verify the influence of sawdust substrates obtained from wood treated with sodium tribromophenate and not treated of pinus elliotti, p. taeda, and araucaria angustifolia in larval cultures of ovine gastrintestinal helminths. feces samples were collected between 8 to 12am, from a lamb naturally infected with gastrintestinal nematodes and analysed by the modified mc master technique for worm egg counting (epg). six groups of 20g of feces were prepared. each group recei ... | 2006 | 17196125 |
| biflavonoids from brazilian pine araucaria angustifolia as potentials protective agents against dna damage and lipoperoxidation. | a biflavonoid fraction (bff) obtained from araucaria angustifolia needles was effective to quench singlet oxygen (1o2), to protect plasmid dna against single strand break (ssb) caused by 1o2 or fenton reaction and to inhibit fenton or uv radiation-induced lipoperoxidation in phosphatidylcholine liposomes. the activity of the biflavonoid fraction (bff) was compared with quercetin, rutin (flavonoids), ginkgetin, amentoflavone (biflavonoids), alpha-tocopherol and trolox. the bff displayed a higher ... | 2004 | 16153416 |
| purification and characterization of two major lectins from araucaria brasiliensis syn. araucaria angustifolia seeds (pinhão). | two major lectins (lectin i and lectin ii) were purified to homogeneity from the seeds of araucaria brasiliensis (gymnospermae). the purity of the lectins was confirmed by polyacrylamide gel electrophoresis, isoelectric focusing, and high performance liquid chromatography. they are glycoproteins in nature containing 6.3 and 2.9%, respectively, of neutral sugar and have absorption coefficients of 3.8 and 4.7, respectively, at 280 nanometers. the molecular weights of both lectins obtained by gel f ... | 1991 | 16668523 |
| purification and biological effects of araucaria angustifolia (araucariaceae) seed lectin. | this paper describes the purification and characterization of a new n-acetyl-d-glucosamine-specific lectin from araucaria angustifolia (aal) seeds (araucariaceae) and its anti-inflammatory and antibacterial activities. aal was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on sephacel-deae. the pure protein has 8.0kda (sds-page) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent ca ... | 2006 | 17045568 |
| sporulation and diversity of arbuscular mycorrhizal fungi in brazil pine in the field and in the greenhouse. | the aim of this work was to assess the sporulation and diversity of arbuscular mycorrhizal fungi (amf) at different forest sites with araucaria angustifolia (bert.) o. ktze. (brazil pine). in addition, a greenhouse experiment was carried out to test the use of traditional trap plants (maize + peanut) or a. angustifolia to estimate the diversity of amf at each site. soil samples were taken in two state parks at southwestern brazil: campos do jordão (parque estadual de campos do jordão [pecj]) and ... | 2007 | 17342509 |
| genetic structure of araucaria angustifolia (araucariaceae) populations in brazil: implications for the in situ conservation of genetic resources. | the distribution of the genetic variation within and among natural populations of a. angustifolia growing in different regions in brazil was assessed at microsatellite and aflp markers. both markers revealed high gene diversity ( h = 0.65; ar = 9.1 for microsatellites and h = 0.27; p = 77.8 % for aflps), moderate overall differentiation ( rst = 0.13 for microsatellites and fst = 0.10 for aflps), but high divergence of the northernmost, geographically isolated population. in a bayesian analysis, ... | 2007 | 17401807 |
| the role of gene flow in shaping genetic structures of the subtropical conifer species araucaria angustifolia. | the morphological features of pollen and seed of araucaria angustifolia have led to the proposal of limited gene dispersal for this species. we used nuclear microsatellite and aflp markers to assess patterns of genetic variation in six natural populations at the intra- and inter-population level, and related our findings to gene dispersal in this species. estimates of both fine-scale spatial genetic structure (sgs) and migration rate suggest relatively short-distance gene dispersal. however, gen ... | 2008 | 18426482 |
| patterns of pollen and seed dispersal in a small, fragmented population of the wind-pollinated tree araucaria angustifolia in southern brazil. | paternity analysis based on eight microsatellite loci was used to investigate pollen and seed dispersal patterns of the dioecious wind-pollinated tree, araucaria angustifolia. the study sites were a 5.4 ha isolated forest fragment and a small tree group situated 1.7 km away, located in paranalpha state, brazil. in the forest fragment, 121 males, 99 females, 66 seedlings and 92 juveniles were mapped and genotyped, together with 210 seeds. in the tree group, nine male and two female adults were ma ... | 2007 | 17928810 |
| evidences of delayed size recovery in araucaria angustifolia populations after post-glacial colonization of highlands in southeastern brazil. | up to date, little is known about the relationship between historical demography and the current genetic structure of a. angustifolia. as a first effort towards overcoming this lack, microsatellite data scored in six populations and isozyme allele frequencies published for 11 natural stands of this species were analysed in order to assess molecular signatures of populations' demographic history. signatures of genetic bottlenecks were captured in all analysed populations of southeastern brazil. a ... | 2008 | 18797796 |
| biflavonoids from araucaria angustifolia protect against dna uv-induced damage. | ultraviolet radiation is one of the most deleterious forms of radiation to terrestrial organisms and is involved in formation of mutagenic pyrimidine dimers and oxidized nucleotides. the biflavonoid fraction (bff), extracted from needles of araucaria angustifolia was capable of protecting calf thymus dna from damage induced by uv radiation. this occurred through prevention of cyclobutane thymine dimer and 8-oxo-7,8-dihydro-2'-deoxyguanosine formation, this being quantified by high-performance li ... | 2009 | 19349053 |
| applications of brazilian pine-fruit shell in natural and carbonized forms as adsorbents to removal of methylene blue from aqueous solutions--kinetic and equilibrium study. | the brazilian pine-fruit shell (araucaria angustifolia) is a food residue, which was used in natural and carbonized forms, as low-cost adsorbents for the removal of methylene blue (mb) from aqueous solutions. chemical treatment of brazilian pine-fruit shell (pw), with sulfuric acid produced a non-activated carbonaceous material (c-pw). both pw and c-pw were tested as low-cost adsorbents for the removal of mb from aqueous effluents. it was observed that c-pw leaded to a remarkable increase in the ... | 2009 | 18930589 |
| central action of araucaria angustifolia seed lectin in mice. | possible central nervous system effects of the gymnosperm lectin from araucaria angustifolia seeds were studied in seizure and open field tests. male swiss mice were administered saline (control), lectin (0.1, 1, and 10 mg/kg), flumazenil (1 mg/kg), or diazepam (1 mg/kg) intraperitoneally. lectin at the highest dose increased time to death in the pentylenetetrazole- and strychnine-induced seizure models as compared with control, but not in the pilocarpine model. in the open field test, lectin re ... | 2009 | 19446042 |
| changes in the 2-de protein profile during zygotic embryogenesis in the brazilian pine (araucaria angustifolia). | araucaria angustifolia is the only native conifer of economic importance in the brazilian atlantic rainforest. due to a clear-cutting form of exploitation this species has received the status of vulnerable. the aim of this work was to investigate and characterize changes in protein expression profile during seed development of this endangered species. for this, the proteome of developing seeds was characterized by 2-de and lc-ms/ms. ninety six proteins were confidently identified and classified ... | 2009 | 19367732 |
| effects of understory structure on the abundance, richness and diversity of collembola (arthropoda) in southern brazil. | the purpose of this study was to investigate the effects of different landscape structures on the understory collembola community. four different forest physiognomies were compared: pinus spp. plantation, eucalyptus spp. plantation, araucaria angustifolia plantation, and a remaining native araucaria forest. three areas containing two sampling units (25 mx2 m each) were selected in each forest physiognomy. understory collembola collection was done with a 1x1 m canvas sheet held horizontally below ... | 2009 | 19618049 |
| new species of stropharia from araucaria angustifolia forests of southern brazil. | two new agaric species of stropharia are described from araucaria angustifolia (brazilian pine) forests of southern brazil. stropharia agaricoides presents agaricus-like basidiomes, small basidiospores (5-7 microm long) and pyriform cheilochrysocystidia. stropharia venusta is characterized by the reddish to pinkish pileus, large basidiospores (12-15 microm long) and subclavate cheiloleptocystidia. | 2009 | 19623934 |
| antiviral activity-guided fractionation from araucaria angustifolia leaves extract. | araucaria angustifolia (bert.) o. kuntze (araucariaceae) is a brazilian medicinal plant traditionally used for the treatment of various illnesses including dried skin, wounds, shingles, and sexually transmitted diseases. | 2009 | 19761825 |
| hosts, distribution and genetic divergence (16s rdna) of amblyomma dubitatum (acari: ixodidae). | we supply information about hosts and distribution of amblyomma dubitatum. in addition, we carry out an analysis of genetic divergence among specimens of a. dubitatum from different localities and with respect to other neotropical amblyomma species, using sequences of 16s rdna gene. although specimens of a. dubitatum were collected on several mammal species as cattle horse, tapirus terrestris, mazama gouazoubira, tayassu pecari, sus scrofa, cerdocyon thous, myocastor coypus, allouata caraya, glo ... | 2010 | 20084537 |
| monoicy in a. angustifolia (bert.) o. kuntze (araucariaceae): i. morphological aspects of the reproductive structures. | araucaria angustifolia (bert.) o. kuntze is a dioecious conifer species native of brazil. the rare occurrence of monoiceous specimens have been attributed to pathogenic infections or other injuries in adult trees. here, the morphological characteristics of male and female cones and pollen grains of a monoiceous a. angustifolia are described. male and female cones and pollen grains presented normal morphology, lacking any sort of injuries or infection and suggesting the existence of further groun ... | 2009 | 19893896 |
| isolation, selection and characterization of root-associated growth promoting bacteria in brazil pine (araucaria angustifolia). | araucaria angustifolia, a unique species of this genus that occurs naturally in brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. root-associated bacteria from a. angustifolia were isolated, selected and characterized for their biotechnological potential of growth promotion and biocontrol of plant pathogenic fungi. ninety-seven strains were isolated and subje ... | 2011 | 21596540 |
| structure of mixed ombrophyllous forests with araucaria angustifolia (araucariaceae) under external stress in southern brazil. | this study is part of the floristic and forest inventory of santa catarina, conceived to evaluate forest resources, species composition and structure of forest remnants, providing information to update forest conservation and land use policy in southern brazilian state of santa catarina (95 000 km2). in accordance to the brazilian national forest inventory (ifn-br), the inventory applies systematic sampling, with 440 clusters containing four crosswise 1 000m2 plots (20 x 50m) each, located on a ... | 2011 | 22017139 |
| identification and characterisation of serine protease inhibitors from araucaria angustifolia seeds. | araucaria angustifolia seeds are characterised by a relatively high content of starch and protein. this study aimed to verify the presence of α-amylase inhibitors in the seeds and to characterise a trypsin inhibitor found in the embryo tissues. inhibitor purification was carried out by the saline extraction of proteins, acetone precipitation and affinity chromatography. two protein bands of molecular weight estimated by sds-page at about 35 kda were further examined by high-performance liquid ch ... | 2016 | 26873059 |
| somatic embryogenesis in araucaria angustifolia (bertol.) kuntze (araucariaceae). | this chapter deals with the features of somatic embryogenesis (se) in araucaria angustifolia, an endangered and native conifer from south brazil. in this species se includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (pems), which precede somatic embryos development. a. angustifolia se model encompasses induction, proliferation, pre-maturation, and maturation steps. double-staining with acetocarmine and evan's blue is useful to evaluate the embryon ... | 2016 | 26619879 |
| quantitative proteomic analysis of araucaria angustifolia (bertol.) kuntze cell lines with contrasting embryogenic potential. | gelc–ms/ms based label free proteomic profiling was used in the large scale identification and quantification of proteins from brazilian pine (araucaria angustifolia) embryogenic cell (ec) lines that showed different propensities to form somatic embryos. using a predicted protein sequence database that was derived from a. angustifolia rna-seq data, 2398 non-redundant proteins were identified. the log2 of the spectral count values of 858 proteins of these proteins showed a normal distribution, an ... | 2016 | 26435419 |
| identification and evaluation of reference genes for quantitative analysis of brazilian pine (araucaria angustifolia bertol. kuntze) gene expression. | quantitative analysis of gene expression is a fundamental experimental approach in many fields of plant biology, but it requires the use of internal controls representing constitutively expressed genes for reliable transcript quantification. in this study, we identified fifteen putative reference genes from an a. angustifolia transcriptome database. variation in transcript levels was first evaluated in silico by comparing read counts and then by quantitative real-time pcr (qrt-pcr), resulting in ... | 2015 | 26313945 |
| inhibition of pancreatic lipase and triacylglycerol intestinal absorption by a pinhão coat (araucaria angustifolia) extract rich in condensed tannin. | the purpose of the present work was to characterize the possible inhibition of pancreatic lipase by a tannin-rich extract obtained from the pinhão (araucaria angustifolia seed) coat, based on the previous observation that this preparation inhibits α-amylases. kinetic measurements of pancreatic lipase revealed that the pinhão coat tannin is an effective inhibitor. inhibition was of the parabolic non-competitive type. the inhibition constants, ki1 and ki2, were equal to 332.7 ± 146.1 μg/ml and 321 ... | 2015 | 26184295 |
| cultural landscapes of the araucaria forests in the northern plateau of santa catarina, brazil. | the araucaria forest is associated with the atlantic forest domain and is a typical ecosystem of southern brazil. the expansion of araucaria angustifolia had a human influence in southern brazil, where historically hunter-gatherer communities used the pinhão, araucaria's seed, as a food source. in the north of the state of santa catarina, the araucaria forest is a mosaic composed of cultivation and pasture inserted between forest fragments, where pinhão and erva-mate are gathered; some local com ... | 2015 | 26054395 |
| effects of single and dual physical modifications on pinhão starch. | pinhão starch was modified by annealing (ann), heat-moisture (hmt) or sonication (snt) treatments. the starch was also modified by a combination of these treatments (ann-hmt, ann-snt, hmt-ann, hmt-snt, snt-ann, snt-hmt). whole starch and debranched starch fractions were analyzed by gel-permeation chromatography. moreover, crystallinity, morphology, swelling power, solubility, pasting and gelatinization characteristics were evaluated. native and single ann and snt-treated starches exhibited a ca- ... | 2015 | 25977003 |
| toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of araucaria angustifolia (bert.) o. kuntze. | somatic embryogenesis is a morphogenetic route useful for the study of embryonic development, as well as the large-scale propagation of endangered species, such as the brazilian pine (araucaria angustifolia). in the present study, we investigated the morphological and ultrastructural organization of a. angustifolia somatic embryo development by means of optical and electron microscopy. the proembryogenic stage was characterized by the proliferation of proembryogenic masses (pems), which are cell ... | 2016 | 25968333 |
| mitochondria and redox homoeostasis as chemotherapeutic targets of araucaria angustifolia (bert.) o. kuntze in human larynx hep-2 cancer cells. | natural products are among one of the most promising fields in finding new molecular targets in cancer therapy. laryngeal carcinoma is one of the most common cancers affecting the head and neck regions, and is associated with high morbidity rate if left untreated. the aim of this study was to examine the antiproliferative effect of araucaria angustifolia on laryngeal carcinoma hep-2 cells. the results showed that a. angustifolia extract (aae) induced a significant cytotoxicity in hep-2 cells com ... | 2015 | 25770932 |
| control of resin production in araucaria angustifolia, an ancient south american conifer. | araucaria angustifolia is an ancient slow-growing conifer that characterises parts of the southern atlantic forest biome, currently listed as a critically endangered species. the species also produces bark resin, although the factors controlling its resinosis are largely unknown. to better understand this defence-related process, we examined the resin exudation response of a. angustifolia upon treatment with well-known chemical stimulators used in fast-growing conifers producing both bark and wo ... | 2015 | 25545585 |
| a new species of swamp rat of the genus scapteromys waterhouse, 1837 (rodentia: sigmodontinae) endemic to araucaria angustifolia forest in southern brazil. | a new species of swamp rat of the genus scapteromys from the meridional plateau of southern brazil is described. morphological, molecular, and karyological analysis support the recognition of the new species, distinct from s. aquaticus and s. tumidus. scapteromys sp. nov. is significantly smaller than the congeneric taxa considering most of the external and craniometric measurements and the pelage is conspicuously grayer and darker. it can be distinguished from s. tumidus by the laterally extend ... | 2014 | 24943159 |
| forest biomass variation in southernmost brazil: the impact of araucaria trees. | a variety of environmental and biotic factors determine vegetation growth and affect plant biomass accumulation. from temperature to species composition, aboveground biomass storage in forest ecosystems is influenced by a number of variables and usually presents a high spatial variability. with this focus, the aim of the study was to evaluate the variables affecting live aboveground forest biomass (agb) in subtropical moist forests of southern brazil, and to analyze the spatial distribution of b ... | 2014 | 24912365 |
| indole-3-acetic acid producing root-associated bacteria on growth of brazil pine (araucaria angustifolia) and slash pine (pinus elliottii). | araucaria forests in brazil today correspond to only 0.7 % of the original 200 km(2) of natural forest that covered a great part of the southern and southeastern area of the atlantic forest and, although araucaria angustifolia is an endangered species, illegal exploitation is still going on. as an alternative to the use of hardwoods, pinus elliottii presents rapid growth and high tolerance to climatic stress and low soil fertility or degraded areas. thus, the objective of this study was to evalu ... | 2014 | 24481491 |
| an improved protocol for intact chloroplasts and cpdna isolation in conifers. | performing chloroplast dna (cpdna) isolation is considered a major challenge among different plant groups, especially conifers. isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. so far, plastid genome sequencing protocols for conifer species have been based mainly on long-range pcr, which is known to be time-consuming and difficult to implement. | 2014 | 24392157 |
| antioxidant and antigenotoxic activities of the brazilian pine araucaria angustifolia (bert.) o. kuntze. | polyphenols are natural products with recognized potential in drug discovery and development. we aimed to evaluate the polyphenolic profile of araucaria angustifolia bracts, and their ability to scavenge reactive species. the antioxidant and antigenotoxic effects of a. angustifolia polyphenols in mrc5 human lung fibroblast cells were also explored. the total polyphenol extract of a. angustifolia was determined by the folin-ciocalteu reagent and the chemical composition was confirmed by hplc. rea ... | 2014 | 26784661 |
| proteomic analysis and polyamines, ethylene and reactive oxygen species levels of araucaria angustifolia (brazilian pine) embryogenic cultures with different embryogenic potential. | somatic embryogenesis is an important biotechnological tool in the large-scale propagation of elite genotypes and ex situ conservation of conifer species. protocols for the induction and proliferation of embryogenic cultures (ecs) of brazilian pine (araucaria angustifolia (bert.) o. ktze) are well established, although the proper formation of mature somatic embryos (ses) is still problematic. thus, the identification of molecular markers for the screening of ecs able to respond to maturation con ... | 2014 | 24327423 |
| growth inhibition of an araucaria angustifolia (coniferopsida) fungal seed pathogen, neofusicoccum parvum, by soil streptomycetes. | araucariaceae are important forest trees of the southern hemisphere. life expectancy of their seedlings can largely be reduced by fungal infections. in this study we have isolated and characterized such a fungus and investigated the potential of streptomyces actinobacteria from the respective rhizosphere to act as antagonists. | 2013 | 23866024 |
| on the use of data mining for estimating carbon storage in the trees. | forests contribute to climate change mitigation by storing carbon in tree biomass. the amount of carbon stored in this carbon pool is estimated by using either allometric equations or biomass expansion factors. both of the methods provide estimate of the carbon stock based on the biometric parameters of a model tree. this study calls attention to the potential advantages of the data mining technique known as instance-based classification, which is not used currently for this purpose. the analysi ... | 2013 | 23758745 |
| immunoanalysis of dehydrins in araucaria angustifolia embryos. | the aim of this study was to describe the dehydrin content of mature araucaria angustifolia embryos, a species of endangered and economically important conifers, native to southern brazil, northeastern argentina, and eastern paraguay. the a. angustifolia seeds have been categorized as recalcitrant. dehydrins were studied by western blot analysis and in situ immunolocalization microscopy using antibodies raised against the k segment, a highly conserved lysine-rich 15-amino acid sequence extensive ... | 2013 | 23263687 |
| the involvement of pump from mitochondria of araucaria angustifolia embryogenic cells in response to cold stress. | in this study, the responses of plant uncoupling mitochondrial protein (pump) and alternative oxidase (aox) in mitochondria from embryogenic cells of a. angustifolia subjected to cold stress (4°c for 24 h or 48 h) is reported. in the mitochondria of stressed cells, pump activity increased by approximately 45% (at 24h and 48 h), which was determined by measuring the oxygen consumption after the addition of linoleic acid and the inhibition by bsa and atp. pump activation was confirmed using transm ... | 2012 | 23116675 |
| structural and component characterization of meristem cells in araucaria angustifolia (bert.) o. kuntze zygotic embryo. | araucaria angustifolia, the brazilian pine, is an endangered native conifer with economic and ecological importance. the female cone develops seeds containing the zygotic embryo, which, at cotyledonary stage, shows well-developed meristems. little is known about the structure of gymnosperm meristems. in the present work, the composition and morphological organization of araucaria angustifolia shoot and root apical meristems were studied during embryo development, using histochemical and microsco ... | 2013 | 23014896 |
| polyamines affect the cellular growth and structure of pro-embryogenic masses in araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines. | polyamines (pas) are abundant polycationic compounds involved in many physiological processes in plants, including somatic embryogenesis. this study investigates the role of pas on cellular growth and structure of pro-embryogenic masses (pems), endogenous pa and proton pump activities in embryogenic suspension cultures of araucaria angustifolia. the embryogenic suspension cultures were incubated with putrescine (put), spermidine (spd), spermine (spm) and the inhibitor methylglyoxal-bis(guanylhyd ... | 2013 | 22998677 |
| glutathione improves early somatic embryogenesis in araucaria angustifolia (bert) o. kuntze by alteration in nitric oxide emission. | in this work, it was observed a straight relationship between the manipulation of the reduced glutathione (gsh)/glutathione disulfide (gssg) ratio, nitric oxide emission and quality and number of early somatic embryos in araucaria angustifolia, a brazilian endangered native conifer. in low concentrations gsh (0.01 and 0.1mm) is a potential no scavenger in the culture medium. furthermore, it can increase the number of early se formed in cell suspension culture media in a few days. however, the ma ... | 2012 | 22921001 |
| study on the stability of β-carotene microencapsulated with pinhão (araucaria angustifolia seeds) starch. | native and hydrolyzed pinhão starches were used as coating materials for β-carotene microencapsulation by freeze-drying. the purpose of the present study was to evaluate the stability of β-carotene encapsulated under three different conditions: in the presence of ultraviolet light at 25±2 °c, in the dark at 25±2 °c and in the dark at 10.0±0.2 °c. the color of the samples was also analyzed. microcapsules prepared with native starch showed the lowest stability during storage. in contrast, microcap ... | 2012 | 24750928 |
| the periodic wetting of leaves enhances water relations and growth of the long-lived conifer araucaria angustifolia. | the importance of foliar absorption of water and atmospheric solutes in conifers was recognised in the 1970s, and the importance of fog as a water source in forest environments has been recently demonstrated. araucaria angustifolia (araucariaceae) is an emergent tree species that grows in montane forests of southern brazil, where rainfall and fog are frequent events, leading to frequent wetting of the leaves. despite anatomical evidence in favour of leaf water absorption, there is no information ... | 2013 | 22672733 |
| female parthenogenetic apomixis and androsporogenetic parthenogenesis in embryonal cells of araucaria angustifolia: interpolation of progenesis and asexual heterospory in an artificial sporangium. | cell fate, development timing and occurrence of reproductive versus apomictic development in gymnosperms are shown to be influenced by culture conditions in vitro. in this study, female parthenogenetic apomixis (fpa), androsporogenetic parthenogenesis (map) and progenesis were demonstrated using embryonal initials of araucaria angustifolia in scaled-up cell suspensions passing through a single-cell bottleneck in darkness and in an artificial sporangium (as). expression was based on defined nutri ... | 2012 | 22669467 |
| cloning and expression of embryogenesis-regulating genes in araucaria angustifolia (bert.) o. kuntze (brazilian pine). | angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. few studies have covered molecular aspects of embryogenesis in the brazilian pine, the only economically important native conifer in brazil. thus eight embryogenesis-regulat ... | 2012 | 22481892 |
| polyamines, iaa and aba during germination in two recalcitrant seeds: araucaria angustifolia (gymnosperm) and ocotea odorifera (angiosperm). | plant growth regulators play an important role in seed germination. however, much of the current knowledge about their function during seed germination was obtained using orthodox seeds as model systems, and there is a paucity of information about the role of plant growth regulators during germination of recalcitrant seeds. in the present work, two endangered woody species with recalcitrant seeds, araucaria angustifolia (gymnosperm) and ocotea odorifera (angiosperm), native to the atlantic rain ... | 2011 | 21685432 |
| differential proteome analysis of mature and germinated embryos of araucaria angustifolia. | araucaria angustifolia is an endangered brazilian native conifer tree. the aim of the present work was to identify differentially expressed proteins between mature and germinated embryos of a. angustifolia, using one and two dimensional gel electrophoresis approaches followed by protein identification by tandem mass spectrometry. the identities of 32 differentially expressed protein spots from two dimensional gel maps were successfully determined, including proteins and enzymes involved in stora ... | 2011 | 21276992 |
| antioxidant activity and low cytotoxicity of extracts and isolated compounds from araucaria angustifolia dead bark. | the antioxidant activities of the extract and its relevant fraction as well as isolated compounds from the dead bark of araucaria angustifolia are presented. this tree represents the mixed ombrophile forest, which is endangered due to extensive logging. the dead bark of araucaria is naturally discarded by the tree, and its hydroalcoholic crude extract has exhibited protective qualities against stress induced by h(2)o(2) in cell culture. using several in vitro models, here we describe the antioxi ... | 2010 | 21208522 |
| erratum to: toward establishing a morphological and ultrastructural characterization of proembryogenic masses and early somatic embryos of araucaria angustifolia (bert.) o. kuntze. | | 2017 | 28133703 |
| elucidation of the polyamine biosynthesis pathway during brazilian pine (araucaria angustifolia) seed development. | | 2017 | 28175909 |
| influence of extrusion cooking on in vitro digestibility, physical and sensory properties of brazilian pine seeds flour (araucaria angustifolia). | brazilian pine seeds (pinhão) are gluten-free products derived from araucaria angustifolia. the commercialization of these seeds is essentially associated with a low level of industrialization. in this context, extrusion cooking is a potential alternative for preparing extrudates of pinhão as a food product, which can be easily digested and is ready for human consumption. brazilian pine seeds flour was processed in a single-screw extruder following a central composite rotatable design. three fac ... | 2017 | 28339105 |
| mps1 (monopolar spindle 1) protein inhibition affects cellular growth and pro-embryogenic masses morphology in embryogenic cultures of araucaria angustifolia (araucariaceae). | somatic embryogenesis has been shown to be an efficient tool for studying processes based on cell growth and development. the fine regulation of the cell cycle is essential for proper embryo formation during the process of somatic embryogenesis. the aims of the present work were to identify and perform a structural and functional characterization of mps1 and to analyze the effects of the inhibition of this protein on cellular growth and pro-embryogenic mass (pem) morphology in embryogenic cultur ... | 2016 | 27064899 |
| elucidation of the polyamine biosynthesis pathway during brazilian pine (araucaria angustifolia) seed development. | polyamines (pas), such as spermidine and spermine, as well as amino acids that are substrates for their biosynthesis, are known to be essential for plant development. however, little is known about the gene expression and metabolic switches associated with the ornithine/arginine and pa biosynthetic pathway during seed development in conifers. to understand these metabolic switches, the enzyme activity of arginine decarboxylase and ornithine decarboxylase, as well as the contents of pas and amino ... | 2016 | 27885170 |
| endangered plant-parrot mutualisms: seed tolerance to predation makes parrots pervasive dispersers of the parana pine. | parrots are largely considered plant antagonists as they usually destroy the seeds they feed on. however, there is evidence that parrots may also act as seed dispersers. we evaluated the dual role of parrots as predators and dispersers of the critically endangered parana pine (araucaria angustifolia). eight of nine parrot species predated seeds from 48% of 526 parana pines surveyed. observations of the commonest parrot indicated that 22.5% of the picked seeds were dispersed by carrying them in t ... | 2016 | 27546381 |
| arabinogalactan-proteins from cell suspension cultures of araucaria angustifolia. | arabinogalactan-proteins (agps), found in the culture medium of suspension cells of araucaria angustifolia grown in plant growth regulator-free and plant growth regulator-containing bm media, bm0 and bm2, respectively, were evaluated quantitatively and qualitatively. the concentrated extracellular fractions (cefs), obtained from suspension cell cultures grown for 20 days in bm0 and bm2 media yielded two fractions, cef-0 and cef-2, respectively. cef-0 and cef-2 was submitted to selective precipit ... | 2010 | 20488499 |
| application of carbon adsorbents prepared from brazilian-pine fruit shell for the removal of reactive orange 16 from aqueous solution: kinetic, equilibrium, and thermodynamic studies. | activated (ac-pw) and non-activated (c-pw) carbonaceous materials were prepared from the brazilian-pine fruit shell (araucaria angustifolia) and tested as adsorbents for the removal of reactive orange 16 dye (ro-16) from aqueous effluents. the effects of shaking time, adsorbent dosage and ph on the adsorption capacity were studied. ro-16 uptake was favorable at ph values ranging from 2.0 to 3.0 and from 2.0 to 7.0 for c-pw and ac-pw, respectively. the contact time required to obtain the equilibr ... | 2010 | 20398999 |
| patterns of genetic diversity in southern and southeastern araucaria angustifolia (bert.) o. kuntze relict populations. | habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. for the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern brazil) of araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern brazil), was tested. the 673 aflps markers revealed a high level of genetic diversity for the species (ht = 0.27), despite anthropogenic ... | 2009 | 21637518 |
| partially acetylated chitosan oligo- and polymers induce an oxidative burst in suspension cultured cells of the gymnosperm araucaria angustifolia. | suspension-cultured cells were used to analyze the activation of defense responses in the conifer a. angustifolia , using as an elicitor purified chitosan polymers of different degrees of acetylation (da 1-69%), chitin oligomers of different degrees of polymerization (dp 3-6), and chitosan oligomer of different da (0-91%). suspension cultured cells elicited with chitosan polymers reacted with a rapid and transient generation of h2o2, with chitosans of high da (60 and 69%) being the most active o ... | 2008 | 19053293 |
| effect of the alkaline treatment on the ultrastructure of c-type starch granules. | the effect of alkaline treatment on the ultrastructure of c-type starch granules was investigated during the alkaline extraction of araucaria angustifolia (pinhao) starch. the efficiency in protein removal was evaluated using intrinsic fluorescence and kjeldahl's method. in parallel, morphological changes of starch granules were observed using scanning electron microscopy and atomic force microscopy. the starch crystallinity was monitored by wide-angle x-ray scattering and the lamellar structure ... | 2008 | 18517249 |
| development of mucilage cells of araucaria angustifolia (araucariaceae). | the roles of mucilage cells were investigated through morphological and cytological analysis during leaf development in young araucaria angustifolia plants. differentiation began in leaf primordia in the shoot apex, when the young cells underwent a greater increase in volume in comparison with other mesophyll cells. the mucilage polysaccharides were synthesized by dictyosomes, from where they were taken by large vesicles and released into a cavity formed by detachment of the tonoplast, which was ... | 2008 | 18239849 |
| application of brazilian pine-fruit shell as a biosorbent to removal of reactive red 194 textile dye from aqueous solution kinetics and equilibrium study. | the brazilian pine-fruit shell (araucaria angustifolia) is a food residue, that was used as biosorbent for the removal of non-hydrolyzed reactive red 194 (nrr) and hydrolyzed reactive red 194 (hrr) forms from aqueous solutions. chemical treatment of brazilian pine-fruit shell (pw), with chromium (cr-pw), with acid (a-pw), and with acid followed by chromium (cr-a-pw) were also tested as alternative biosorbents for the removal of nrr and hrr from aqueous effluents. it was observed that the treatme ... | 2008 | 18178307 |
| occurrence of "mammalian" lignans in plant and water sources. | enterolignans, also called "mammalian" lignans because they are formed in the intestine of mammals after ingestion of plant lignans, were identified for the first time in extracts of four tree species, i.e., in knot heartwood of the hardwood species fagus sylvatica and in knot or stem heartwood of the softwood species araucaria angustifolia, picea smithiana, and abies cilicia. they were also identified for the first time in grain extracts of cultivated plants, i.e., in 15 cereal species, in 3 nu ... | 2007 | 17497165 |
| internal genetic structure and outcrossing rate in a natural population of araucaria angustifolia (bert.) o. kuntze. | the internal genetic structure and outcrossing rate of a population of araucaria angustifolia (bert.) o. kuntze were investigated using 16 allozyme loci. estimates of the mean number of alleles per loci (1.6), percentage of polymorphic loci (43.8%), and expected genetic diversity (0.170) were similar to those obtained for other gymnosperms. the analysis of spatial autocorrelation demonstrated the presence of internal structure in the first distance classes (up to 70 m), suggesting the presence o ... | 2008 | 16982668 |
| adsorption of cu(ii) on araucaria angustifolia wastes: determination of the optimal conditions by statistic design of experiments. | wastes of araucaria angustifolia (named pinhão) natural (pw) and also loaded with congo red (crp) were tested as low-cost adsorbents for cu(ii) removal from aqueous solutions. in order to reduce the total number of experiments to achieve the best conditions of the batch adsorption procedure, three sets of statistical designs of experiments were carried-out for each adsorbent. initially, a full 2(4) factorial design for each adsorbent with two central points (18 experiments) were performed, to op ... | 2007 | 16876938 |
| changes in cell wall composition associated with maturation in the gymnosperm araucaria angustifolia. | a general structural characterization and an investigation on the dynamics of formation of cell wall polysaccharides was performed, using plantlets stem samples from a typical gymnosperm from southern brazil, araucaria angustifolia, as experimental model. microscopic examination and monosaccharide composition of plantlet segments at different heights were carried out to show the representative portions of stem cell wall development. the plantlets were divided in portions (tip, middle and base) w ... | 2006 | 16542718 |
| statistical design of experiments as a tool for optimizing the batch conditions to cr(vi) biosorption on araucaria angustifolia wastes. | in order to reduce the total number of experiments for achieving the best conditions for cr(vi) uptake using araucaria angustifolia (named pinhão) wastes as a biosorbent, three statistical design of experiments were carried out. a full 2(4) factorial design with two blocks and two central points (20 experiments) was experimented (ph, initial metallic ion concentration-c(o), biosorbent concentration-x and time of contact-t), showing that all the factors were significant; besides, several interact ... | 2006 | 16297543 |
| lignans in resin of araucaria angustifolia by gas chromatography/mass spectrometry. | total extract of resin from araucaria angustifolia was analyzed by gas chromatography/mass spectrometry and 32 lignans were identified. lignan acetates are present in the resin and consist of four secoisolariciresinol acetates, six lariciresinol acetates, two 7'-hydroxylariciresinol acetates and an isolariciresinol acetate, which have hitherto not been reported in the plant kingdom. shonanin and 7'-hydroxylariciresinol type lignans are also present in a. angustifolia resin. lignans containing sy ... | 2004 | 15532064 |
| chemical composition and glycemic index of brazilian pine (araucaria angustifolia) seeds. | the seeds of parana pine (araucaria brasiliensis syn. araucaria angustifolia), named pinhão, are consumed after cooking and posterior dehulling, or they are used to prepare a flour employed in regional dishes. native people that live in the south of brazil usually consume baked pinhão. as a result of cooking, the white seeds become brown on the surface due to the migration of some tinted compounds present in the seed coat. in this work, the proximate composition, minerals, flavonoids, and glycem ... | 2004 | 15161207 |
| endogenous protein phosphorylation and casein kinase activity during seed development in araucaria angustifolia. | protein kinases and phosphatases are responsible for several cellular events mediated by protein phosphorylation and dephosphorylation. among these events are cell growth and differentiation and cellular metabolism. casein kinase i (cki) and casein kinase ii (ckii) are involved in the phosphorylation of several substrates. endogenous protein phosphorylation and casein kinase activity were investigated in the megagametophyte of the native brazilian conifer araucaria angustifolia, during seed deve ... | 2002 | 12453576 |
| phenylpropanoid derivatives and biflavones at different stages of differentiation and development of araucaria angustifolia. | chemical investigations carried out with tissues at different developmental stages of araucaria angustifolia established the presence of e and z isomers of octadecyl p-coumarate and octadecyl ferulate in undifferentiated callus; in the seedling stems, the source of explants, three biflavones of the amentoflavone-type were isolated, whereas the diterpene, trans-communic acid, was obtained from the seedling roots. adult stems accumulated the benzaldehydes, vanillin, p-hydroxybenzaldehyde and conif ... | 2000 | 11130667 |
| the steam volatile oil of wollemia nobilis and its comparison with other members of the araucariaceae (agathis and araucaria). | the leaf essential oil of wollemia nobilis (wollemi pine) has been investigated and compared with other members of the family araucariaceae. all araucaroids examined yielded steam volatile oils in low yields. the oil from wollemia nobilis was composed mainly of (+)-16-kaurene (60%), together with alpha-pinene (9%) and germacrene-d (8%). oils from agathis species endemic to australia were high in monoterpenes, in contrast to those isolated from extra-australian species. the major constituents of ... | 2000 | 10793256 |
| germination-associated events and the desiccation sensitivity of recalcitrant seeds - a study on three unrelated species. | the storage behaviour of recalcitrant seeds was assessed using three diverse species: a gymnosperm, araucaria angustifolia (bert.) o. kuntze; a herbaceous monocotyledon, scadoxus membranaceus (bak.) friis nordal; and a woody dicotyledon, landolphia kirkii dyer. seeds were stored under conditions of high relative humidities that maintained seed moisture content and under low relative humidities that caused drying. at regular intervals moisture content was determined, germinability assessed and th ... | 1989 | 24212748 |
| the multiple forms of alpha-amylase enzyme of the araucaria species of south america: a. araucana (mol.) koch and a. angustifolia (bert.) o. kutz : a comparative study. | alpha-amylase is one of the major enzymes present in the seeds of both araucaria species of south america and it initiates starch hydrolysis during germination and early seedling growth. the pattern of the multiple forms of alpha-amylase of the two araucaria species was investigated by electrophoresis and isoelectrofocusing of the native enzyme in polyacrylamide gels. the enzyme forms were compared in the embryo and megagametophyte of quiescent seeds and of seeds imbibed for 18, 48, and 90 hours ... | 1986 | 16664944 |