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molecular characterization of the genome of maize rayado fino virus, the type member of the genus marafivirus.the complete nucleotide sequence of the single-stranded rna genome of maize rayado fino virus (mrfv), the type member of the genus marafivirus, is 6305 nucleotides (nts) in length and contains two putative open reading frames (orfs). the largest orf (nt 97-6180) encodes a polyprotein of 224 kda with sequence similarities at its n-terminus to the replication-associated proteins of other viruses with positive-strand rna genomes and to the papainlike protease domain found in tymoviruses. the c-term ...200111289816
first infectious clone of the propagatively transmitted oat blue dwarf virus.oat blue dwarf virus (obdv) is a small, phloem-limited marafivirus that replicates in its leafhopper vector. we have developed complete cdna clones of obdv from which infectious transcripts may be derived--the first such clones for any propagatively transmitted plant virus. prior to clone construction, the reported sequences of the 5' and 3' ends were confirmed using 5' race, primer extension, and ligation-anchored pcr. using vascular puncture of maize seeds with capped transcripts, multiple clo ...201020224895
infectious maize rayado fino virus from cloned cdna.a full-length cdna clone was produced from a u.s. isolate of maize rayado fino virus (mrfv), the type member of the genus marafivirus within the family tymoviridae. infectivity of transcripts derived from cdna clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors dalbulus maidis and graminella nigrifrons that transmit the virus in a persistent-propagative manner. infection of maize plants through vascular puncture inocul ...201525651051
identification and molecular characterization of a marafivirus in rubus spp.an undescribed virus with isometric particles and a diameter of ca. 30 nm was identified in diseased samples of wild and cultivated rubus species and molecularly characterized. its genome was 6,463 nt, excluding the 3'-terminal poly(a) tail, and contained a single open reading frame coding for a 2,035-amino-acid-long precursor polypeptide (p223). the amino terminal portion of p223, identified as a replication-associated polyprotein, contained conserved motifs of methyltransferase, endopeptidase/ ...200919784756
coat protein expression strategy of oat blue dwarf virus.oat blue dwarf virus (obdv) is a member of the genus marafivirus whose genome encodes a 227 kda polyprotein (p227) ostensibly processed post-translationally into its functional components. encoded near the 3' terminus and coterminal with the p227 orf are orfs specifying major and minor capsid proteins (cp). since the cp expression strategy of marafiviruses has not been thoroughly investigated, we produced a series of point mutants in the obdv cp encoding gene and examined expression in protoplas ...201424503092
the complete nucleotide sequence and genomic characterization of grapevine asteroid mosaic associated virus.in analyzing grapevine clones infected with grapevine red blotch associated virus, we identified a small number of isometric particles of approximately 30nm in diameter from an enriched fraction of leaf extract. a dominant protein of 25kda was isolated from this fraction using sds-page and was identified by mass spectrometry as belonging to grapevine asteroid mosaic associated virus (gamav). using a combination of three methods rna-seq, srna-seq, and sanger sequencing of rt- and race-pcr product ...201727720957
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