| [detection of human papillomavirus (hpv) dna in carcinoma of uterine cervix and genital tract diseases in jiangsu province, china]. | pst-1 cleaved dna restriction fragment length polymorphism (rflp) of biopsy samples from 41 patients with squamous cell carcinoma of uterine cervix collected in jiangsu province, china were examined for hpvs by southern blot hybridization using hpv 16 dna as a probe. 20 of the 41 samples were positive for hpvs when hybridized under non-stringent condition. hpv was not detectable in samples collected in the same time period from patients with cervical adenocarcinoma (n = 2), vaginal carcinoma (n ... | 1992 | 1349522 |
| detection of human papilloma virus (hpv) in laryngeal carcinoma tissue. | using alpha-32p-dctp labelled human papillomavirus type 16 dna genome probe (chpv 16 dna, cloned and identified from a chinese cervical invasive carcinoma), 3 hpv 16 genomic relative sequences were detected in 6 biopsies of laryngeal carcinoma. the results showed serious hpv infection in laryngeal carcinoma. this indicates that hpv infection may play an important pathogenetic role in cancer development. | 1991 | 1651831 |
| [detection of human papillomavirus (hpv) type 16 dna relative sequence in the tissue of laryngeal carcinoma]. | by using alpha 32-p-dctp labeled chinese human papillomavirus type 16 dna (chpv 16 dna) probe, we have adopted southern blot method and detected hpv subtype 16 dna (1/6) and hpv 16 dna relative sequence (3/6) in 6 biopsy specimens of laryngeal carcinoma. the result showed a severe infection of hpv in laryngeal carcinoma. this indicates that hpv infection may play a pathogenetic role in the cancer. | 1991 | 1653582 |
| the glycoprotein genes and gene junctions of the fish rhabdoviruses spring viremia of carp virus and hirame rhabdovirus: analysis of relationships with other rhabdoviruses. | the nucleotide sequences of the glycoprotein genes and all of the internal gene junctions of the fish pathogenic rhabdoviruses spring viremia of carp virus (svcv) and hirame rhabdovirus (hirrv) have been determined from cdna clones generated from viral genomic rna. the svcv glycoprotein gene sequence is 1588 nucleotides (nt) long and encodes a 509 amino acid (aa) protein. the hirrv glycoprotein gene sequence comprises 1612 nt, coding for a 508 aa protein. in sequence comparisons of 15 rhabdoviru ... | 1996 | 8806175 |
| virions of heliothis armigera entomopoxvirus contain a homologue of the vaccinia vp8 major core protein. | an antigenic 30 k virion protein of heliothis armigera entomopoxvirus (haepv) has been identified as a homologue of the chordopoxvirus (chpv) vp8 major virion core protein. like its homologue in vaccinia virus, the mature haepv 30 k protein is derived by post-translational cleavage of a precursor at a conserved aga motif. the haepv 30 k protein is the first epv structural virion protein to be described, and elucidation of its characteristics provides evidence for the assumption that morphologica ... | 1999 | 10499447 |
| matrix protein of chandipura virus inhibits transcription from an rna polymerase ii promoter. | chandipura virus (chpv) is a vesiculovirus, related to, but phylogenetically distinct from, vesicular stomatitis virus (vsv). the matrix protein of vsv, as well as its role in virus assembly, inhibits the transcription from promoters for host rna polymerases i and ii. cloning and expression of the matrix protein of chpv in human cells showed that this protein is also functional in its inhibitory effect on transcription of a reporter gene from the cytomegalovirus immediate-early promoter, despite ... | 1999 | 10595413 |
| the genome of fowlpox virus. | here we present the genomic sequence, with analysis, of a pathogenic fowlpox virus (fpv). the 288-kbp fpv genome consists of a central coding region bounded by identical 9.5-kbp inverted terminal repeats and contains 260 open reading frames, of which 101 exhibit similarity to genes of known function. comparison of the fpv genome with those of other chordopoxviruses (chpvs) revealed 65 conserved gene homologues, encoding proteins involved in transcription and mrna biogenesis, nucleotide metabolis ... | 2000 | 10729156 |
| poxvirus genomes: a phylogenetic analysis. | the evolutionary relationships of 26 sequenced members of the poxvirus family have been investigated by comparing their genome organization and gene content and by using dna and protein sequences for phylogenetic analyses. the central region of the genome of chordopoxviruses (chpvs) is highly conserved in gene content and arrangement, except for some gene inversions in fowlpox virus (fpv) and species-specific gene insertions in fpv and molluscum contagiosum virus (mcv). in the central region 90 ... | 2004 | 14718625 |
| purification and partial characterization of an entomopoxvirus (dlepv) from a parasitic wasp of tephritid fruit flies. | an insect poxvirus [entomopoxvirus (epv)] occurs in the poison gland apparatus of female diachasmimorpha longicaudata, a parasitic wasp of the caribbean fruit fly, anastrepha suspensa and other tephritid fruit flies. the dlepv virion is 250-300 nm in diameter, has a "bumpy" appearance and a unipartite double stranded dna genome of 290-300 kb. dlepv dna restriction fragment profiles differed from those reported for amsacta moorei epv (amepv) and melanoplus sanguinipes epv (msepv), the only two ep ... | 2002 | 15455044 |
| complete genome sequences of chandipura and isfahan vesiculoviruses. | chandipura virus (chpv) and isfahan virus (isfv) are two members of the genus vesiculovirus from asia. both are arthropod-transmitted and are able to infect humans, but neither causes vesicular stomatitis in livestock. the complete genome sequence for each virus has been determined. the negative-sense rna genome comprises 11,119 nt (chpv) or 11,088 nt (isfv). the most variable of the non-transcribed regions is the intergenic spacer at the g-l gene junction (4 bases in isfv, 20 in chpv). phylogen ... | 2004 | 15614433 |
| detection of chandipura virus from sand flies in the genus sergentomyia (diptera: phlebotomidae) at karimnagar district, andhra pradesh, india. | a total of 191 adult sand flies belonging to the genus sergentomyia were collected from seven villages in karimnagar and warangal districts of andhra pradesh state, india, after an outbreak of encephalitis due to chandipura virus (chpv). fifteen pools, each containing two specimens, were tested by reverse transcriptase-polymerase chain reaction and sequencing. one pool of sergentomyia from kolanur village in karimnagar district was positive for chpv. | 2005 | 15962804 |
| propagation of chandipura virus in chick embryos. | stocks of three indian chandipura virus (chpv) isolates; one isolate from an adult febrile case in 1965 from chandipura town, maharashtra, and two isolates from two pediatric encephalitis cases from andhra pradesh, 2003 were inoculated in 10-day-old chick embryos by allantoic route. all three virus isolates replicated in chick embryos showing titre of log 10(12) to log 10(13) eid50. the results demonstrated that chick embryos are susceptible to chpv and virus grows to high titres in this system. ... | 2005 | 16235730 |
| vertical and venereal transmission of chandipura virus (rhabdoviridae) by aedes aegypti (diptera: culicidae). | experiments in the laboratory documented vertical and venereal transmission of chandipura virus (chpv) in aedes aegypti (l.). the minimum filial infection rate among the progeny of infected females was 1.2%; the rate among male and female progeny was 0.9 and 1.4%, respectively. the venereal infection rate of chpv among inseminated females was 32.7%. our study indicates the possible occurrence of vertical and venereal transmission of chpv in insect vectors. | 2005 | 16363175 |
| genetic characterization of the capra hircus papillomavirus: a novel close-to-root artiodactyl papillomavirus. | the healthy skin of a seven-year-old female goat (capra hircus) was sampled with a cotton tip swab. total genomic dna was extracted from the sample and subjected to multiply primed rolling-circle amplification (rca). restriction analysis showed that the complete genome of a papillomavirus was amplified, measuring approximately 7,600 bp in length. the capra hircus papillomavirus (chpv-1) genome was cloned from this rolling-circle amplification product, and the complete nucleotide sequence was det ... | 2006 | 16430985 |
| genome of crocodilepox virus. | here, we present the genome sequence, with analysis, of a poxvirus infecting nile crocodiles (crocodylus niloticus) (crocodilepox virus; crv). the genome is 190,054 bp (62% g+c) and predicted to contain 173 genes encoding proteins of 53 to 1,941 amino acids. the central genomic region contains genes conserved and generally colinear with those of other chordopoxviruses (chpvs). crv is distinct, as the terminal 33-kbp (left) and 13-kbp (right) genomic regions are largely crv specific, containing 4 ... | 2006 | 16641289 |
| venereal transmission of chandipura virus by phlebotomus papatasi (scopoli). | experiments were conducted in the laboratory on phlebotomus papatasi to determine the possible role of males in maintaining or sustaining the chandipura virus (chpv) activity in nature. this study indicated that infected males are capable of passing on the virus to female sand flies while mating. the infection rate was found to be 12.5% in uninfected females when mated with infected males. the occurrence of venereal transmission of this virus may have epidemiologic importance in the natural cycl ... | 2006 | 17172384 |
| experimental transmission of chandipura virus by phlebotomus argentipes (diptera: psychodidae). | experiments were carried out to demonstrate the susceptibity and transmission potential of phlebotomus argentipes (annandale & brunetti) for chandipura virus (chpv). in india, p. argentipes is one of the predominant species found in many areas endemic for chpv. although its laboratory colonization is difficult, we have demonstrated that 65% of p. argentipes were susceptible to chpv infection by the oral route. transmission experiments were carried out by intrathoracic inoculation because of re-f ... | 2007 | 17297040 |
| chandipura virus: a major cause of acute encephalitis in children in north telangana, andhra pradesh, india. | a hospital-based surveillance was undertaken between may 2005 and april 2006 to elucidate the contribution of chandipura virus (chpv) to acute viral encephalitis cases in children, seroconversion in recovered cases and to compare the seroprevalences of anti-chpv igm and n antibodies in areas reporting cases with those without any case of acute viral encephalitis. during this period, 90 cases of acute encephalitis were hospitalized in the pediatric wards of mahatma gandhi memorial (mgm) hospital, ... | 2008 | 18041027 |
| immune response during acute chandipura viral infection in experimentally infected susceptible mice. | age dependent susceptibility was observed in chandipura virus (chpv) infected mice through intravenous and intraperitoneal route. adult mice were susceptible only through intracerebral route of infection. immature neuron and some other biological variables including immature immune system are considered to be important factor for age related susceptibility in some diseases. as chandipura virus infects both young and adult mice brain through intracerebral route the role of immune system during pe ... | 2008 | 18937835 |
| quantification of n-(3-chloro-2-hydroxypropyl)valine in human haemoglobin as a biomarker of epichlorohydrin exposure by gas chromatography-tandem mass spectrometry with stable-isotope dilution. | epichlorohydrin (ech) is an important industrial intermediate for the production of polymers and surface coatings. animal experiments support the classification of ech as a carcinogen, and a significant contribution to the cancer risk of ech exposed humans has to be considered. upon uptake, epichlorohydrin reacts with nucleophilic moieties of n- and s-containing macromolecules to form stable adducts, e.g. with haemoglobin. in this article, we describe a gc-tandem ms method for the quantitative a ... | 2009 | 19059011 |
| development and evaluation of a real-time one step reverse-transcriptase pcr for quantitation of chandipura virus. | chandipura virus (chpv), a member of family rhabdoviridae was attributed to an explosive outbreak of acute encephalitis in children in andhra pradesh, india in 2003 and a small outbreak among tribal children from gujarat, western india in 2004. the case-fatality rate ranged from 55-75%. considering the rapid progression of the disease and high mortality, a highly sensitive method for quantifying chpv rna by real-time one step reverse transcriptase pcr (real-time one step rt-pcr) using taqman tec ... | 2008 | 19091082 |
| recombinant glycoprotein based vaccine for chandipura virus infection. | chandipura virus (chpv) has emerged as an important pediatric encephalitis-causing pathogen with very high mortality in india. no specific vaccine or treatment is available till date. we attempted to prepare a candidate vaccine employing recombinant chpv glycoprotein (rgp). the glycoprotein gene (g-gene) of chpv was expressed using baculovirus expression system. the rgp was purified by hplc and used for mice immunization, 3 doses, and 4 weeks apart. one microgram rgp was found to be optimum. ser ... | 2009 | 19428894 |
| bioinformatics comparison of g protein of isfahan virus with the same proteins of two other closely related viruses of the genus vesiculovirus. | since there are not much experimental data available about different structural properties of isfahan virus (isfv), in the present investigation, computational study of g protein of isfv was performed and the results were compared with g proteins of chandipura virus (chpv) and piry virus (pv). calculation of amino acid compositions of g proteins of viruses was done by pseaac server. predictions of localization, sequence of signal peptides, c, n and o glycosylation sites, transmembrane helices, c ... | 2009 | 19508221 |
| characterization of the structure of the phosphoprotein of chandipura virus, a negative stranded rna virus probing intratryptophan energy transfer using single and double tryptophan mutants. | the phosphoprotein (p protein) of chandipura virus (chpv), a negative stranded rna virus, is involved in both transcription and replication phases of the viral life cycle. the two tryptophan (trp) residues of chpv, located at 105 and 135 respectively and two single trp mutants w135f and w105f and a double trp mutant w135f/w105f have been characterized by steady state and time-resolved fluorescence and phosphorescence at 298 k and 77 k. results indicate that trp135 is more buried with less polar ... | 2010 | 19895867 |
| evaluation of the immunogenicity of a recombinant glycoprotein-based chandipura vaccine in combination with commercially available dpt vaccine. | chandipura virus (chpv) belongs to family rhabdovoridae and has emerged as an encephalitis causing pathogen with high mortality among pediatric population from three indian states. the recombinant glycoprotein (rgp) was shown to be an excellent vaccine candidate as evaluated in a murine model. as the disease is predominantly rural, to ensure maximum coverage for chandipura vaccine, an attempt was made to evaluate combination of rgp and a commercially available dpt vaccine (chp-dpt). when chp-dpt ... | 2010 | 20005854 |
| intracranial administration of p gene sirna protects mice from lethal chandipura virus encephalitis. | in parts of india, chandipura virus (chpv) has emerged as an encephalitis causing pathogen in both epidemic and sporadic forms. this pediatric disease follows rapid course leading to 55-75% mortality. in the absence of specific treatment, effectiveness of rna interference (rnai) was evaluated. | 2010 | 20062542 |
| development of an enzyme-linked immunosorbent assay to detect chicken parvovirus-specific antibodies. | here we report the development and application of an enzyme-linked immunosorbent assay (elisa) to detect parvovirus-specific antibodies in chicken sera. we used an approach previously described for other parvoviruses to clone and express viral structural proteins in insect cells from recombinant baculovirus vectors. in baculovirus recombinant-infected sf9 cells, the chicken parvovirus (chpv) structural viral protein 2 (vp2) was detected as an abundant protein, and the 60-kda vp2 strongly reacted ... | 2009 | 20095161 |
| determination and analysis of the full-length chicken parvovirus genome. | viral enteric disease in poultry is an ongoing problem in many parts of the world. many enteric viruses have been identified in turkeys and chickens, including avian astroviruses, rotaviruses, reoviruses, and coronaviruses. through the application of a molecular screening method targeting particle-associated nucleic acid (pan), we recently described the detection and partial characterization of a novel enteric parvovirus in chickens. subsequent surveys of intestinal homogenates from turkeys and ... | 2010 | 20097398 |
| the hr motif in the rna-dependent rna polymerase l protein of chandipura virus is required for unconventional mrna-capping activity. | chandipura virus (chpv) is an emerging human pathogen associated with acute encephalitis and is related closely to vesicular stomatitis virus (vsv), a prototype rhabdovirus. here, we demonstrate that the rna polymerase l protein of chpv exhibits a vsv-like rna:gdp polyribonucleotidyltransferase (prntase) activity, which transfers the 5'-monophosphorylated (p-) viral mrna start sequence to gdp to produce a capped rna, and that the conserved hr motif in the chpv l protein is essential for the prnt ... | 2010 | 20107017 |
| chandipura virus growth kinetics in vertebrate cell lines, insect cell lines & embryonated eggs. | since not much information on chandipura virus is available, an attempt was made to study the growth kinetics of the virus in certain vertebrate, invertebrate cell lines and embryonated chicken eggs. | 2010 | 20716815 |
| chandipura virus encephalitis outbreak among children in nagpur division, maharashtra, 2007. | an outbreak of acute encephalitis syndrome (aes) among children from nagpur division, maharashtra was investigated to confirm the aetiology and to describe clinico-epidemiological features. | 2010 | 20966517 |
| chipmunk parvovirus is distinct from members in the genus erythrovirus of the family parvoviridae. | the transcription profile of chipmunk parvovirus (chppv), a tentative member of the genus erythrovirus in the subfamily parvovirinae of the family parvoviridae, was characterized by transfecting a nearly full-length genome. we found that it is unique from the profiles of human parvovirus b19 and simian parvovirus, the members in the genus erythrovirus so far characterized, in that the small rna transcripts were not processed for encoding small non-structural proteins. however, like the large non ... | 2010 | 21151930 |
| naturally occurring parvoviral infection in hungarian broiler flocks. | the major enteric disease (ed) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. viruses from numerous families have been identified in the intestinal tracts of poultry with ed, such as astroviridae, coronaviridae, reoviridae, rotaviridae, and parvoviridae. the objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (chpv) and turkey parvovirus (tupv) in hungarian flocks ... | 2011 | 21500039 |
| reviewing host proteins of rhabdoviridae: possible leads for lesser studied viruses. | rhabdoviridae, characterized by bullet-shaped viruses, is known for its diverse host range, which includes plants, arthropods, fishes and humans. understanding the viral-host interactions of this family can prove beneficial in developing effective therapeutic strategies. the host proteins interacting with animal rhabdoviruses have been reviewed in this report. several important host proteins commonly interacting with animal rhabdoviruses are being reported, some of which, interestingly, have mo ... | 2011 | 22116291 |
| high prevalence of turkey parvovirus in turkey flocks from hungary experiencing enteric disease syndromes. | samples collected in 2008 and 2009, from 49 turkey flocks of 6 to 43 days in age and presenting clinical signs of enteric disease and high mortality, were tested by polymerase chain reaction and reverse transcription-polymerase chain reaction for the presence of viruses currently associated with enteric disease (ed) syndromes: astrovirus, reovirus, rotavirus, coronavirus, adenovirus, and parvovirus. turkey astroviruses were found in 83.67% of the cases and turkey astrovirus 2 (tast-2) in 26.53%. ... | 2011 | 22017049 |
| whole genomes of chandipura virus isolates and comparative analysis with other rhabdoviruses. | the chandipura virus (chpv) belonging to the vesiculovirus genus and rhabdoviridae family, has recently been associated with a number of encephalitis epidemics, with high mortality in children, in different parts of india. no full length genome sequences of chpv isolates were available in genbank and little is known about the molecular markers for pathogenesis. in the present study, we provide the complete genomic sequences of four isolates from epidemics during 2003-2007. these sequences along ... | 2012 | 22272333 |
| comparative analysis of recombinant human papillomavirus 8 l1 production in plants by a variety of expression systems and purification methods. | human papillomavirus 8 (hpv-8), one of the high-risk cutaneous papillomaviruses (chpvs), is associated with epidermodysplasia verruciformis and nonmelanoma skin cancer in immuno-compromised individuals. currently, no vaccines against chpvs have been reported; however, recent studies on cross-neutralizing properties of their capsid proteins (cp) have fostered an interest in vaccine production against these viruses. we examined the potential of producing hpv-8 major cp l1 in nicotiana benthamiana ... | 2012 | 22260326 |
| establishment of cell line from embryonic tissue of pipistrellus ceylonicus bat species from india & its susceptibility to different viruses. | pipistrellus ceylonicus bat species is widely distributed in south asia, with additional populations recorded in china and southeast asia. bats are the natural reservoir hosts for a number of emerging zoonotic diseases. attempts to isolate bat-borne viruses in various terrestrial mammalian cell lines have sometimes been unsuccessful. the bat cell lines are useful in isolation and propagation of many of the viruses harboured by bats. new stable bat cell lines are needed to help such investigation ... | 0 | 24056599 |
| chandipura virus induces neuronal death through fas-mediated extrinsic apoptotic pathway. | chandipura virus (chpv; genus vesiculovirus, family rhabdoviridae) is an emerging tropical pathogen with a case fatality rate of 55 to 75% that predominantly affects children in the age group of 2 to 16 years. although it has been established as a neurotropic virus causing encephalitis, the molecular pathology leading to neuronal death is unknown. the present study elucidates for the first time the mechanism of cell death in neurons after chpv infection that answers the basic cause of chpv-media ... | 2013 | 24027318 |
| isolation of chandipura virus (vesiculovirus: rhabdoviridae) from sergentomyia species of sandflies from nagpur, maharashtra, india. | an outbreak of acute encephalitis syndrome was reported from vidarbha region of maharashtra s0 tate, india, during july 2012. anti-igm antibodies against chandipura virus (chpv) were detected in clinical samples. sandfly collections were done to determine their role in chpv transmission. | 0 | 25027088 |
| elucidating the interacting domains of chandipura virus nucleocapsid protein. | the nucleocapsid (n) protein of chandipura virus (chpv) plays a crucial role in viral life cycle, besides being an important structural component of the virion through proper organization of its interactions with other viral proteins. in a recent study, the authors had mapped the associations among chpv proteins and shown that n protein interacts with four of the viral proteins: n, phosphoprotein (p), matrix protein (m), and glycoprotein (g). the present study aimed to distinguish the regions of ... | 2013 | 24288532 |
| monocytes and b cells support active replication of chandipura virus. | interaction between immune system and chandipura virus (chpv) during different stages of its life cycle remain poorly understood. the exact route of virus entry into the blood and cns invasion has not been clearly defined. the present study was undertaken to assess the population in pbmc that supports the growth of virus and to detect active virus replication in pbmc as well as its subsets. | 2016 | 27628855 |
| changing clinical scenario in chandipura virus infection. | chandipura virus (chpv) (vesiculovirus: rhabdoviridae) garnered global attention as an emerging neurotropic pathogen inflicting high mortality in children within 24 h of commencement of symptoms. the 2003-2004 outbreaks in central india witnessed case fatality rates ranging from 56-75 per cent in andhra pradesh and gujarat with typical encephalitic symptoms. due to the acute sickness and rapid deterioration, the precise mechanism of action of the virus is still unknown. recent studies have shown ... | 0 | 27748295 |
| detection and molecular characterization of chicken astrovirus associated with chicks that have an unusual condition known as "white chicks" in brazil. | chicken astrovirus (castv) is one of many viruses related to enteric diseases in poultry that are associated with runting-stunting syndrome (rss), which affects young chickens. castv was also recently associated with an unusual condition in chicks called "white chicks." some hatcheries in certain states of brazil have reported several incubation problems, mortality, and the presence of chicks with white plumages over the past several months. these chicks were termed locally as "white chicks." th ... | 2016 | 26976903 |
| molecular detection of chicken parvovirus in broilers with enteric disorders presenting curving of duodenal loop, pancreatic atrophy, and mesenteritis. | enteric disorders are an important cause of economic losses in broiler chickens worldwide. several agents have been associated with enteric problems, such as viruses, bacteria, and parasites. in this study, broiler chickens showing signs of enteric disorders were subjected to molecular diagnosis for several viral agents and also for pathological examination for elucidating this problem. thus, the chickens were screened for avian nephritis virus (anv), chicken astrovirus (castv), avian rotavirus ... | 2016 | 26908891 |
| molecular survey of enteric viruses in commercial chicken farms in korea with a history of enteritis. | several enteric viruses have increasingly received attention as potential causative agents of runting-stunting syndrome (rss) in chickens. a molecular survey was performed to determine the presence of a broad range of enteric viruses, namely chicken astrovirus (castv), avian nephritis virus (anv), chicken parvovirus (chpv), infectious bronchitis virus (ibv), avian rotavirus (avrv), avian reovirus (arv), and fowl adenovirus (fadv), in intestinal samples derived from 34 commercial chicken flocks t ... | 2013 | 24135590 |
| development of a novel rapid micro-neutralization elisa for the detection of neutralizing antibodies against chandipura virus. | chandipura virus (chpv) is a leading cause of acute encephalitis with high mortality in paediatric population in india. a micro-neutralization elisa (mn-elisa) assay was developed for the detection of neutralizing antibodies (nab) against chpv. this novel method gives read-out in the form of elisa optical density (od) values and has a shorter turn-around time (tat) as compared to the conventional cytopathic effect (cpe)-based neutralization assay (mn-cpe). the assay was developed using an indian ... | 2017 | 27856212 |
| network analysis reveals common host protein/s modulating pathogenesis of neurotropic viruses. | network analysis through graph theory provides a quantitative approach to characterize specific proteins and their constituent assemblies that underlie host-pathogen interactions. in the present study, graph theory was used to analyze the interactome designed out of 50 differentially expressing proteins from proteomic analysis of chandipura virus (chpv, family: rhabdoviridae) infected mouse brain tissue to identify the primary candidates for intervention. using the measure of degree centrality, ... | 2016 | 27581498 |
| microglial activation induces neuronal death in chandipura virus infection. | neurotropic viruses induce neurodegeneration either directly by activating host death domains or indirectly through host immune response pathways. chandipura virus (chpv) belonging to family rhabdoviridae is ranked among the emerging pathogens of the indian subcontinent. previously we have reported that chpv induces neurodegeneration albeit the root cause of this degeneration is still an open question. in this study we explored the role of microglia following chpv infection. phenotypic analysis ... | 2016 | 26931456 |
| graph theoretic network analysis reveals protein pathways underlying cell death following neurotropic viral infection. | complex protein networks underlie any cellular function. certain proteins play a pivotal role in many network configurations, disruption of whose expression proves fatal to the cell. an efficient method to tease out such key proteins in a network is still unavailable. here, we used graph-theoretic measures on protein-protein interaction data (interactome) to extract biophysically relevant information about individual protein regulation and network properties such as formation of function specifi ... | 2015 | 26404759 |
| chandipura virus perturbs cholesterol homeostasis leading to neuronal apoptosis. | chandipura virus (chpv; genus vesiculovirus, family rhabdoviridae) induces neuronal death through the fas-mediated extrinsic apoptosis pathway. what propels this apoptosis remains unclear, although oxysterols have been reported to be key players in neurodegeneration. in our study of chpv-infected brain samples, we observed over-expression of genes such as apolipoprotein e, cyp46a1, srebf-1 and nsdhl. this backs up the hypothesis that chpv replication demands cholesterol that is supplied by apoli ... | 2015 | 26118540 |
| host interactions of chandipura virus matrix protein. | the rhabdovirus matrix (m) protein is a multifunctional virion protein that plays major role in virus assembly and budding, virus-induced inhibition of host gene expression and cytopathic effects observed in infected cells. the myriad roles played by this protein in the virus biology make it a critical player in viral pathogenesis. therefore, discerning the interactions of this protein with host can greatly facilitate our understanding of virus infections, ultimately leading to both improved the ... | 2015 | 25944354 |
| neuroinvasion by chandipura virus. | chandipura virus (chpv) is an arthropod borne rhabdovirus associated with acute encephalitis in children below the age of 15 years in the tropical states of india. although the entry of the virus into the nervous system is among the crucial events in the pathogenesis of chpv, the exact mechanism allowing chpv to invade the central nervous system (cns) is currently poorly understood. in the present review, based on the knowledge of host interactors previously predicted for chpv, along with the su ... | 2014 | 24713200 |
| a peptide targeted against phosphoprotein and leader rna interaction inhibits growth of chandipura virus -- an emerging rhabdovirus. | the fatal illness caused by chandipura virus (chpv), an emerging pathogen, presently lacks any therapeutic option. previous research suggested that interaction between the virally encoded phosphoprotein (p) and the positive sense leader rna (le-rna) may play an important role in the viral lifecycle. in this report, we have identified a β-sheet/loop motif in the c-terminal domain of the chpv p protein as essential for this interaction. a synthetic peptide encompassing this motif and spanning a co ... | 2013 | 24036128 |
| predicting the host protein interactors of chandipura virus using a structural similarity-based approach. | chandipura virus (chpv), alike other pathogens, exploits the cellular infrastructure of their hosts through complex network of interactions for successful infection. chpv being a recently emerged pediatric encephalitic virus, the mechanisms involved in the establishment of viral persistence are still ill defined. because the protein interface between chpv and its host provides one means by which the virus invades and seize control of their human host machinery, the authors in this study have emp ... | 2013 | 23847124 |
| characterization of the chandipura virus leader rna-phosphoprotein interaction using single tryptophan mutants and its detection in viral infected cells. | the phosphoprotein (p protein) of the chandipura virus (chpv), a negative strand rna virus, is involved in both transcription and replication of the viral life cycle. interaction between the p protein and the viral leader (le) rna under in vitro conditions has been previously reported for chpv and other negative strand rna viruses such as the rinderpest virus (rpv). however, till date, the region of the p protein involved in le rna binding remains undefined. moreover, the in vivo occurrence of t ... | 2013 | 23063516 |
| intraviral protein interactions of chandipura virus. | chandipura virus (chpv) is an emerging rhabdovirus responsible for several outbreaks of fatal encephalitis among children in india. the characteristic structure of the virus is a result of extensive and specific interplay among its five encoded proteins. the revelation of interactions among chpv proteins can help in gaining insight into viral architecture and pathogenesis. in the current study, we carried out comprehensive yeast two-hybrid (y2h) analysis to elucidate intraviral protein-protein i ... | 2012 | 22763614 |
| chandipura virus: an emerging tropical pathogen. | chandipura virus (chpv), a member of rhabdoviridae, is responsible for an explosive outbreak in rural areas of india. it affects mostly children and is characterized by influenza-like illness and neurologic dysfunctions. it is transmitted by vectors such as mosquitoes, ticks and sand flies. an effective real-time one step reverse-transcriptase pcr assay method is adopted for diagnosis of this virus. chpv has a negative sense rna genome encoding five different proteins (n, p, m, g, and l). p prot ... | 2012 | 22721825 |
| interaction of chandipura virus n and p proteins: identification of two mutually exclusive domains of n involved in interaction with p. | the nucleocapsid protein (n) and the phosphoprotein (p) of nonsegmented negative-strand (nns) rna viruses interact with each other to accomplish two crucial events necessary for the viral replication cycle. first, the p protein binds to the aggregation prone nascent n molecules maintaining them in a soluble monomeric (n(0)) form (n(0)-p complex). it is this form that is competent for specific encapsidation of the viral genome. second, the p protein binds to oligomeric n in the nucleoprotein comp ... | 2012 | 22485180 |
| chpvdb--a sequence annotation database for chandipura virus. | databases containing proteomic information have become indispensable for virology studies. as the gap between the amount of sequence information and functional characterization widens, increasing efforts are being directed to the development of databases. for virologist, it is therefore desirable to have a single data collection point which integrates research related data from different domains. chpvdb is our effort to provide virologist such a one-step information center. we describe herein th ... | 2009 | 19293996 |
| emergence of enteric viruses in production chickens is a concern for avian health. | several viruses have been identified in recent years in the intestinal contents of chickens and turkeys with enteric problems, which have been observed in commercial farms worldwide, including brazil. molecular detection of these viruses in brazil can transform to a big threat for poultry production due to risk for intestinal integrity. this disease is characterized by severely delayed growth, low uniformity, lethargy, watery diarrhea, delayed feed consumption, and a decreased conversion rate. c ... | 2014 | 24578633 |
| characterization of the inhibitory effect of an extract of prunella vulgaris on ebola virus glycoprotein (gp)-mediated virus entry and infection. | currently, no approved antiviral therapeutic is available for treatment or prevention of ebola virus (ebov) infection. in this study, we characterized an ebov-glycoprotein (gp) pseudotyped hiv-1-based vector system in different cell cultures, including human umbilical vein endothelial cells (huvecs) and human macrophages, for the screening of anti-ebov-gp agent(s). based on this system, we demonstrated that an aqueous extract (chpv) from the chinese herb prunella vulgaris displayed a potent inhi ... | 2016 | 26778707 |
| incidence of parvoviruses in chickens infected with marek's disease virus. | the aim of the study was to determine co-occurrence of marek's disease virus (mdv) and chicken parvovirus (chpv) co-occurrence in field chicken flocks. the materials for the study derived from 115 broiler chickens or layer hens originated from 23 farms with suspicion of marek's disease (md). dual infection with mdv and chpv was found in 23 (20%) examined chickens. the results obtained suggest a possibility of influence of chpv infection on efficacy of the md vaccines. | 2013 | 24195296 |
| human hemorrhagic fever causing arenaviruses: molecular mechanisms contributing to virus virulence and disease pathogenesis. | arenaviruses include multiple human pathogens ranging from the low-risk lymphocytic choriomeningitis virus (lcmv) to highly virulent hemorrhagic fever (hf) causing viruses such as lassa (lasv), junin (junv), machupo (macv), lujo (lujv), sabia (sabv), guanarito (gtov), and chapare (chpv), for which there are limited preventative and therapeutic measures. why some arenaviruses can cause virulent human infections while others cannot, even though they are isolated from the same rodent hosts, is an e ... | 2015 | 26011826 |
| drug discovery technologies and strategies for machupo virus and other new world arenaviruses. | seven arenaviruses cause viral hemorrhagic fever in humans: the old world arenaviruses lassa and lujo, and the new world clade b arenaviruses machupo (macv), junín (junv), guanarito (gtov), sabiá (sabv), and chapare (chpv). all of these viruses are risk group 4 biosafety pathogens. macv causes human disease outbreak with high case-fatality rates. to date, at least 1,200 cases with ≈200 fatalities have been recorded. | 2012 | 22607481 |
| update on the college of american pathologists experience with high-risk human papillomavirus proficiency testing for cytology. | - since 2008, the college of american pathologists has provided the human papillomavirus for cytology laboratories (chpv) proficiency testing program to help laboratories meet the requirements of the clinical laboratory improvement amendments of 1988. | 2016 | 27557412 |
| age-specific performance of carehpv versus papanicolaou and visual inspection of cervix with acetic acid testing in a primary cervical cancer screening. | human papillomavirus (hpv) is recommended as a primary screening tool for cervical screening. assessment of age-specific performance of newer hpv carehpv dna testing is important as risk of cervical intraepithelial neoplasia (cin) varies at different ages. we aim to evaluate carehpv in comparison to papanicolaou (pap) test and visual inspection of the cervix with acetic acid (via) cervical screening tests for the detection of high-grade cin. | 2016 | 26248549 |
| adjunct screening of cervical or vaginal samples using carehpv testing with pap and aided visual inspection for detecting high-grade cervical intraepithelial neoplasia. | to evaluate the performance of carehpv dna testing for human papillomavirus (hpv) on cervical and vaginal samples (chpv and vhpv respectively) in adjunct screening together with visual inspection of the cervix by acetic acid (via) or papanicolaou (pap) tests in the detection of high-grade cervical intraepithelial neoplasia (cin). | 2015 | 25548076 |
| human papillomavirus viral load on carehpv testing of self-collected vaginal samples vs. clinician-collected cervical samples. | to compare viral load on carehpv dna testing in self-collected vaginal (vhpc) and clinician-collected cervical (chpc) samples for the detection of high-grade cervical intra-epithelial neoplasia (cin). | 2014 | 25171269 |
| the college of american pathologists' first 3 years' experience with high-risk human papillomavirus proficiency testing for cytology and other laboratories. | the college of american pathologists (cap) human papillomavirus (high-risk) survey for cytopathology and other laboratories (chpv) meets the clinical laboratory improvement amendments of 1988 (clia '88) requirements for 5 proficiency testing challenges analyzed 3 times per year. this study reports laboratory performance for chpv from 2008 through 2010. | 2013 | 23627450 |
| vectorial capacity of culex gelidus (theobald) mosquitoes to certain viruses of public health importance in india. | culex gelidus, a widely prevalent mosquito in india and southeast asia region, is an important vector of japanese encephalitis virus (jev). experimental studies have shown its potential to transmit west nile, kunjin, murray valley encephalitis and ross river viruses. an attempt was therefore made to study its susceptibility and vector competence to some of the arboviruses of public health importance in india. | 2015 | 26119548 |
| expression of chicken parvovirus vp2 in chicken embryo fibroblasts requires codon optimization for production of naked dna and vectored meleagrid herpesvirus type 1 vaccines. | meleagrid herpesvirus type 1 (mehv-1) is an ideal vector for the expression of antigens from pathogenic avian organisms in order to generate vaccines. chicken parvovirus (chpv) is a widespread infectious virus that causes serious disease in chickens. it is one of the etiological agents largely suspected in causing runting stunting syndrome (rss) in chickens. initial attempts to express the wild-type gene encoding the capsid protein vp2 of chpv by insertion into the thymidine kinase gene of mehv- ... | 2013 | 23861017 |
| chicken parvovirus viral loads in cloacal swabs from malabsorption syndrome-affected and healthy broilers. | chicken parvovirus (chpv) has been associated with malabsorption syndrome (mas) in broilers. however, the participation of this virus in such syndrome is unclear, since it may be detected in diseased and healthy chickens. in the course of these studies, it was argued whether chpv genome loads might be correlated to the occurrence of mas. to check such a hypothesis, a sybr green-based quantitative polymerase chain reaction was developed to detect and quantify chpv genomes. cloacal swabs from 68 b ... | 2016 | 27627905 |
| genetic and phylogenetic analysis of a novel parvovirus isolated from chickens in guangxi, china. | a previously unidentified chicken parvovirus (chpv) strain, associated with runting-stunting syndrome (rss), is now endemic among chickens in china. to explore the genetic diversity of chpv strains, we determined the first complete genome sequence of a novel chpv isolate (gx-ch-pv-7) identified in chickens in guang xi, china, and showed moderate genome sequence similarity to reference strains. analysis showed that the viral genome sequence is 86.4 %-93.9 % identical to those of other chpvs. gene ... | 2016 | 27503240 |
| chicken parvovirus and its associations with malabsorption syndrome. | malabsorption syndrome (mas) is a multifactorial syndrome which is characterized by enteric disorders and reduced growth rates of broilers. such condition is responsible for significant economic losses to the poultry industry. a possible association between chicken parvovirus (chpv) infections and the occurrence of mas has been proposed. however, such association has not to date been elucidated in view that chpv has been detected in healthy as well as in mas-affected chickens. this study aimed t ... | 2016 | 27473992 |
| host specificity and phylogenetic relationships of chicken and turkey parvoviruses. | inoculation of specific-pathogen-free chickens and turkeys with five chicken parvoviruses (chpv) and five turkey parvoviruses (tupv) resulted in productive virus replication only in the homologous host species. a phylogenetic tree based on nucleotide sequences of the vp1 gene segment revealed a host-specific clustering of the virus strains. these results suggest that the vp1 gene plays an essential role in host specificity of chpv and tupv strains and could be a relevant target sequence for stra ... | 2015 | 26292551 |
| isolation and molecular characterisation of chicken parvovirus from brazilian flocks with enteric disorders. | 1. the presence of parvovirus in chickens with enteric disease was investigated in commercial flocks in brazil. 2. the intestinal contents of chickens exhibiting clinical signs of diarrhoea, weight loss or mortality were examined, and chicken parvovirus (chpv) was identified using a polymerase chain reaction (pcr) assay. the samples were sequenced and inoculated into specific-pathogen-free (spf) embryonated eggs to isolate the virus. 3. necropsies showed that the embryos were dwarfish, haemorrha ... | 2015 | 25633520 |
| genetic characterization of three novel chicken parvovirus strains based on analysis of their coding sequences. | chicken parvovirus (chpv) is one of the causative agents of viral enteritis. recently, the genome of the abu-p1 strain of chpv was fully sequenced and determined to have a distinct genomic composition compared with that of vertebrate parvoviruses. however, no comparative sequence analysis of coding regions of chpvs was possible because of the lack of other sequence information. in this study, we obtained the nucleotide sequences of all genomic coding regions of three chpvs by polymerase chain re ... | 2015 | 25510852 |
| chicken parvovirus-induced runting-stunting syndrome in young broilers. | previously we identified a novel parvovirus from enteric contents of chickens that were affected by enteric diseases. comparative sequence analysis showed that the chicken parvovirus (chpv) represented a new member in the parvoviridae family. here, we describe some of the pathogenic characteristics of chpv in young broilers. following experimental infection, 2-day-old broiler chickens showed characteristic signs of enteric disease. runting-stunting syndrome (rss) was observed in four of five exp ... | 2013 | 23678740 |
| genetic characterization of parvoviruses circulating in turkey and chicken flocks in poland. | between 2008 and 2011, commercial turkey and chicken flocks in poland were examined for the presence of turkey parvovirus (tupv) and chicken parvovirus (chpv). clinical samples (10 individual faecal swabs/flock) from 197 turkey flocks (turkeys aged 1 to 19 weeks) and 45 chicken flocks (chickens aged 3 to 17 weeks) were collected in different regions of the country and tested using a pcr assay that targeted the ns1 gene (3'orf). the prevalence of tupv was 29.4 % in the flocks tested, while chpv i ... | 2012 | 23011307 |
| identification and phylogenetic diversity of parvovirus circulating in commercial chicken and turkey flocks in croatia. | phylogenetic diversity of parvovirus detected in commercial chicken and turkey flocks is described. nine chicken and six turkey flocks from croatian farms were tested for parvovirus presence. intestinal samples from one turkey and seven chicken flocks were found positive, and were sequenced. natural parvovirus infection was more frequently detected in chickens than in turkeys examined in this study. sequence analysis of 400 nucleotide fragments of the nonstructural gene (ns) showed that our sequ ... | 2011 | 22312994 |
| neuropathogenesis by chandipura virus: an acute encephalitis syndrome in india. | chandipura virus (chpv) has been contributing to the rising number of premature deaths due to acute encephalitis syndrome for over a decade in india. chpv belongs to the family rhabdoviridae. neuropathogenesis of chpv has been well established but the exact route of entry into the central nervous system (cns) and the triggering factor for neuronal death are still unknown. rabies virus and vesicular stomatitis virus, which are related closely to chpv, enter the cns retrogradely from peripheral or ... | 2017 | 28731002 |
| genetic characterization of the glycoprotein g of chandipura viruses in india with emphasis on an outbreak of 2015. | chandipura virus (chpv) is found to be associated with sporadic encephalitis outbreaks in humans in india since 1965. we report here, the investigation of chpv activity during the period of june-august 2015 in the state of gujarat, which revealed 24.44% positivity among 45 referred encephalitis cases. phylogenetic study of the g gene sequences of strains from gujarat 2015 along with available sequences of additional strains from different geographical locations and isolation years (1965-2015), i ... | 2017 | 28882516 |
| evaluation of specific humoral and cellular immune responses against the major capsid l1 protein of cutaneous wart-associated alpha-papillomaviruses in solid organ transplant recipients. | infection with different species of cutaneous human papillomaviruses (chpv) of genus alpha (cαhpvs) and associated skin disease are highly prevalent in solid organ transplant recipients (otr), documenting the importance of the immunological control of hpv infection. | 2015 | 25439730 |
| cutaneous papillomavirus infection in patients with rheumatoid arthritis or systemic lupus erythematosus. a case-control study. | previous studies informed an increased prevalence of cutaneous papillomavirus (chpv) infection in patients with systemic lupus erythematosus (sle). the main objective of our study was to evaluate factors associated with chpv infection in patients with either rheumatoid arthritis (ra) or sle, and to determine whether sle itself is an independent risk factor for chpv infection. we included 670 patients (in consecutive selection) in this cross-sectional study (550 with ra and 120 with sle). all pat ... | 2013 | 23722231 |
| assessment of a quick reference table algorithm for determining initial postoperative pressure settings of programmable pressure valves in patients with idiopathic normal pressure hydrocephalus: sinphoni subanalysis. | management of overdrainage complications in shunted patients with idiopathic normal pressure hydrocephalus (inph) remains a difficult task despite the use of programmable pressure valves. | 2012 | 22653392 |
| programmable shunt valves: in vitro assessment of safety of the magnetic field generated by a portable game machine. | cerebrospinal fluid (csf) shunts are frequently used to treat hydrocephalus. the use of a programmable shunt valve allows physicians to easily change the opening pressure. since patients with adjustable csf shunt valves may use portable game machines, the permanent magnets in these machines may alter the shunt valve programmed settings or permanently damage the device. this study investigated the risk of unintentional valve adjustment associated with the use of game machines in patients with pro ... | 2011 | 21946726 |
| [effect of magnetic fields from home-use magnetic induction therapy apparatuses on adjustable cerebrospinal fluid shunt valves]. | cerebrospinal fluid (csf) shunts are frequently used to treat hydrocephalus. the use of a programmable valve allows the operator to easily change the opening pressure. in japan, many people use magnetic induction therapy apparatuses in their homes. however, exposing patients with adjustable csf shunt valves to the permanent magnets included in these apparatuses may alter the shunt valve's programmed settings or permanently damage the device. therefore, the goal of this study was to determine the ... | 2010 | 20697146 |
| overdrainage of cerebrospinal fluid caused by detachment of the pressure control cam in a programmable valve after 3-tesla magnetic resonance imaging. | the authors report a rare case of overdrainage of the csf caused by the malfunction of a codman-hakim programmable valve (chpv) following a 3-t mr imaging procedure. nine years ago this 72-year-old woman underwent ventriculoperitoneal shunt placement with a chpv system for hydrocephalus due to subarachnoid hemorrhage. the postoperative course was uneventful and the system functioned well. a radiograph obtained immediately after 3-t mr imaging revealed that the pressure control cam in the valve s ... | 2010 | 19408984 |
| disparity between adjusted and actual opening cerebrospinal fluid pressure in a patient with the codman hakim programmable valve: occult form of shunt failure due to head banging. case report. | although the codman hakim programmable valve (chpv) system is popular, several problems arising from its design have been described. the authors report an additional cause of shunt failure associated with the use of the chpv system. a 7-year-old girl who had previously undergone placement of a ventriculoperitoneal shunt with the chpv system was admitted in a comatose state. radiography did not demonstrate any breakage or change in the opening pressure of the valve. manual pumping of the valve re ... | 2006 | 17328269 |
| do adjustable shunt valves pressure our budget? a retrospective analysis of 541 implanted codman hakim programmable valves. | based on a retrospective study in which a total of 541 codman hakim programmable valves (chpv) were implanted in 477 patients over a 6-year period, this cost analysis was performed. by using a valve with an adjustable opening pressure, valve exchange to alter the opening pressure and surgical evacuation of subdural haematomas and hygromas can be avoided. dividing the added cost for using the chpv by the cost of implanting a non-programmable hakim valve results in 105.8 valve exchanges, which wou ... | 2001 | 11478056 |