| spirochete from the rabbit tick, haemaphysalis leporispalustris (packard). i. isolation and preliminary characterization. | an unidentified spirochete, referred to as the 277f agent, was isolated from haemaphysalis leporispalustris ticks from two cottontail rabbits by inoculation of the tick suspension into embryonated chicken eggs. because of its minute width (0.1 mu), the organism was difficult to see when stained by the giemsa method, but was readily demonstrated by silver impregnation or fluorescent-antibody procedures. in dark-field microscopy, the spirochetes appeared uniformly and rather tightly coiled, and ex ... | 1968 | 4171295 |
| characterization of spirochetes isolated from ticks (ixodes tanuki, ixodes turdus, and ixodes columnae) and comparison of the sequences with those of borrelia burgdorferi sensu lato strains. | ixodes persulcatus serves as a tick vector for borrelia garinii and borrelia afzelii in japan; however, unidentified spirochetes have been isolated from other species of ticks. in this study, 13 isolates from ticks (6 from ixodes tanuki, 6 from ixodes turdus, and 1 from ixodes columnae) and 3 isolates from voles (clethrionomys rufocanus) were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, rrna gene restriction fragment length polymorphism, partial sequencing of the o ... | 1996 | 8779571 |
| free-living spirochetes from cape cod microbial mats detected by electron microscopy. | spirochetes from microbial mats and anaerobic mud samples collected in salt marshes were studied by light microscopy, whole mount and thin section transmission electron microscopy. enriched in cellobiose-rifampin medium, selective for spirochaeta bajacaliforniensis, seven distinguishable spirochete morphotypes were observed. their diameters ranged from 0.17 micron to > 0.45 micron. six of these morphotypes came from southwest cape cod, massachusetts: five from microcoleus-dominated mat samples c ... | 1996 | 9018691 |
| association of oral spirochetes from sites of periodontal health with development of periodontitis. | the purpose of this investigation was to determine whether the presence of disease-associated bacteria in health-associated plaque correlated with susceptibility to periodontitis over time. sites of periodontal health were identified in 65 adults. six months later (recall 1), plaque was collected from sites that remained in periodontal health, and specific bacteria were detected using monoclonal antibodies in a microscopic assay. the spirochete morphogroup was identified by phase contrast micros ... | 1997 | 9444597 |
| association of oral spirochetes from periodontally healthy sites with development of gingivitis. | the purpose of this investigation was to determine whether the presence of selected disease-associated bacteria in health-associated plaque correlated with future gingivitis. sites of periodontal health were identified in 65 adults. six months later (recall 1) plaque was collected from sites that remained in periodontal health, and 5 species of specific bacteria and pathogen-related oral spirochetes were detected using monoclonal antibodies in a microscopic assay. members of the spirochete morph ... | 1998 | 9609381 |
| adherence to and penetration through endothelial cells by oral treponemes. | oral spirochetes were co-incubated with monolayers of endothelial cells seeded into multiwell plates or onto filters mounted in plastic chambers. attachment was assessed using an enzyme-linked immunosorbent assay and scanning electron microscopy. invasiveness was determined by monitoring media beneath filters within chambers for spirochetes using darkfield microscopy. transmission electron microscopy was used to estimate intercellular and intracellular passage of spirochetes through monolayers. ... | 1999 | 10895695 |
| comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis. | research in the past decade has led to the recognition of brachyspira (formerly serpulina) pilosicoli as the primary etiologic agent of colonic spirochetosis (cs), an emerging cause of colitis in humans and animals. attachment of spirochetes to the epithelial surface of the lower intestine is considered to be the hallmark of cs. however, because b. pilosicoli, b. aalborgi and unclassified flagellated bacteria are found singly or together in humans and non-human primates with cs lesions, attachme ... | 2001 | 11708743 |
| isolation of an unidentified spirochete from hen's eggs after inoculation with liver tissue from hens. | | 1947 | 20341597 |