| characterization of rhizobial isolates of phaseolus vulgaris by staircase electrophoresis of low-molecular-weight rna. | low-molecular-weight (lmw) rna molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in spain. since lmw rna profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from spanish soils presumptively could be characterized as rhizobium etli, rhizobium gallicum, rhizobium giardinii, rhizobium leguminosarum bv. viciae and bv. trifolii, and sinorhizobium fredii. | 2001 | 11157280 |
| conservation of plasmid-encoded traits among bean-nodulating rhizobium species. | rhizobium etli type strain cfn42 contains six plasmids. we analyzed the distribution of genetic markers from some of these plasmids in bean-nodulating strains belonging to different species (rhizobium etli, rhizobium gallicum, rhizobium giardinii, rhizobium leguminosarum, and sinorhizobium fredii). our results indicate that independent of geographic origin, r. etli strains usually share not only the psym plasmid but also other plasmids containing symbiosis-related genes, with a similar organizat ... | 2002 | 11976134 |
| structural determination of the lipo-chitin oligosaccharide nodulation signals produced by rhizobium giardinii bv. giardinii h152. | rhizobium giardinii bv. giardinii is a microsymbiont of plants of the genus phaseolus and produces extracellular signal molecules that are able to induce deformation of root hairs and nodule organogenesis. we report here the structures of seven lipochitooligosaccharide (lco) signal molecules secreted by r. giardinii bv. giardinii h152. six of them are pentamers of glcnac carrying c 16:0, c 18:0, c 20:0 and c 18:1 fatty acyl chains on the non-reducing terminal residue. four are sulfated at c-6 of ... | 2003 | 12543556 |
| rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. | a polyphasic study was carried out to determine the taxonomic position of two aerobic, cyanide-degrading bacterial strains, designated l61t and l22, which had been isolated from a bioreactor for the treatment of nickel-complexed cyanide. the two isolates exhibited almost identical taxonomic characteristics. phylogenetic analysis inferred from comparative 16s rrna gene sequences indicated that the isolates fall in a sublineage of the genus rhizobium comprising the type strains of rhizobium giardi ... | 2005 | 16280524 |
| genetic characterization of fast-growing rhizobia able to nodulate prosopis alba in north spain. | prosopis is a mimosaceae legume tree indigenous to south america and not naturalized in europe. in this work 18 rhizobial strains nodulating prosopis alba roots were isolated from a soil in north spain that belong to eight different randomly amplified polymorphic dna groups phylogenetically related to sinorhizobium medicae, sinorhizobium meliloti and rhizobium giardinii according to their intergenic spacer and 16s rrna gene sequences. the nodc genes of isolates close to s. medicae and s. melilot ... | 2007 | 18031342 |
| different species and symbiotic genotypes of field rhizobia can nodulate phaseolus vulgaris in tunisian soils. | abstract a collection of 160 isolates of rhizobia nodulating phaseolus vulgaris in three geographical regions in tunisia was characterized by restriction fragment length polymorphism analysis of polymerase chain reaction (pcr)-amplified 16s rdna, nifh and nodc genes. nine groups of rhizobia were delineated: rhizobium gallicum biovar (bv.) gallicum, rhizobium leguminosarum bv. phaseoli and bv. viciae, rhizobium etli bv. phaseoli, rhizobium giardinii bv. giardinii, and four groups related to speci ... | 2002 | 19709241 |
| sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (phaseolus vulgaris l.) in a northern tunisian field. | a total of 40 symbiotic bacterial strains isolated from root nodules of common bean grown in a soil located in the north of tunisia were characterized by pcr-rflp of the 16s rrna genes. six different ribotypes were revealed. nine representative isolates were submitted to phylogenetic analyses of rrs, reca, atpd, dnak, nifh and noda genes. the strains 23c40 and 23c95 representing the most abundant ribotype were closely related to sinorhizobium americanum cfnei 156(t). s. americanum was isolated f ... | 2012 | 22633818 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| rhizobium gallicum sp. nov. and rhizobium giardinii sp. nov., from phaseolus vulgaris nodules. | thirty-one strains of two new genomic species (genomic species 1 and 2) of rhizobia isolated from root nodules of phaseolus vulgaris and originating from various locations in france were compared with reference strains of rhizobia by performing a numerical analysis of 64 phenotypic features. each genomic species formed a distinct phenon and was separated from the other rhizobial species. a comparison of the complete 16s rrna gene sequences of a representative of genomic species 1 (strain r602spt ... | 1997 | 9336898 |
| influence of host cultivars and rhizobium species on the growth and symbiotic performance of phaseolus vulgaris under salt stress. | in order to study the effect of salt stress on the rhizobium-common bean symbiosis, we investigated the response of both partners, separately and in symbiosis. the comparison of the behaviour of five cultivars of phaseolus vulgaris differing in seed colour, growing on nitrates and different concentrations of nacl, showed genotypic variation with respect to salt tolerance. coco blanc was the most sensitive cultivar, whereas smv 29-21 was the most tolerant one. at the rhizobium level, two strains ... | 2005 | 16255168 |
| isolation and characterization of an early colonizing rhizobium sp. r8 from a household toilet bowl. | the bacterial community structure was compared between the third days', one week', and three weeks' biofilm samples from the surface of a household toilet bowl. it was found that the pcr-dgge band pattern of 16s rrna gene was dramatically changed after the third day and was not further changed until three weeks. this result suggests that there are early and late colonizing bacterial groups. one of the early colonizers isolated from the third days' sample was rhizobium sp. r8, a closest relative ... | 2015 | 25707633 |
| rhizobium gei sp. nov., a bacterial endophyte of geum aleppicum. | a bacterial strain, designated as zfjt-2t, was isolated from the stem of geum aleppicum jacq. collected from taibai mountain in shaanxi province, north-west china. cells of strain zfjt-2t were gram-stain-negative, strictly aerobic, rod-shaped and motile by means of a single polar flagellum. the major fatty acids were summed feature 8 (comprising c18 : 1ω7c and/or c18 : 1ω6c), c16 : 0, 11-methyl c18 : 1ω7c and summed feature 3 (comprising c16 : 1ω7c and/or c16 : 1ω6c), and the dna g+c content was ... | 2016 | 27474082 |
| taxonomy of rhizobia and agrobacteria from the rhizobiaceae family in light of genomics. | phylogenomic analyses showed two major superclades within the family rhizobiaceae that corresponded to the rhizobium/agrobacterium and shinella/ensifer groups. within the rhizobium/agrobacterium group, four highly supported clades were evident that could correspond to distinct genera. the shinella/ensifer group encompassed not only the genera shinella and ensifer but also a separate clade containing the type strain of rhizobium giardinii. ensifer adhaerens (casida a(t)) was an outlier within its ... | 2015 | 25660942 |
| Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China. | A total of 159 endophytic bacteria were isolated from surface-sterilized root nodules of wild perennial Glycyrrhiza legumes growing on 40 sites in central and northwestern China. Amplified fragment length polymorphism (AFLP) genomic fingerprinting and sequencing of partial 16S rRNA genes revealed that the collection mainly consisted of Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium and Paenibacillus species. Based on symbiotic properties with the legume hosts Glycyrrhiza uralensis and Gl ... | 2011 | 22066910 |
| rhizobium halophytocola sp. nov., a novel bacterium isolated from the root of a coastal dune plant. | during a study of endophytic bacteria from coastal dune plants, a bacterial strain, designated yc6881t, was isolated from the root of rosa rugosa collected from the coastal dune areas of namhae island. the bacterium was found to be gram-negative, motile, halophilic and heterotrophic with a polar single flagellum. strain yc6881t exhibited growth at temperature 4-37 °c (optimum at 28-32 °c), ph 6.0-9.0 (optimum at ph 7.0-8.0) and was able to grow at nacl concentrations in the range of 0-7.5 % (w/v ... | 2011 | 22021574 |
| rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of sphaerophysa salsula in china. | four gram-negative, aerobic, motile, non-spore, forming rods with a wide ph and temperature range for growth (ph 7.0-11.0, optimum ph 8.0; 20-45°c, optimum 28°c) strains were isolated from root nodules of sphaerophysa salsula and characterized by means of a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences revealed that the four strains formed a new lineage related to the genus rhizobium and the sequence similarities between the isolate and the most related type strain ... | 2011 | 21308410 |
| diverse rhizobia associated with sophora alopecuroides grown in different regions of loess plateau in china. | a total of seventy-five symbiotic bacterial strains isolated from root nodules of wild sophora alopecuroides grown in different regions of china's loess plateau were characterized. based on the combined rflp patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including mesorhizobium alhagi and m. gobiense as the main groups, as well as agrobacterium tumefaciens, m. amorphae, phyllobacterium trifolii, rhizobium giardinii, r. indigofe ... | 2010 | 20965680 |
| rhizobium herbae sp. nov. and rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in china. | seven rhizobium strains associated with various legume species grown in different geographic regions of china were defined as four genomic groups related to rhizobium giardinii based upon ribosomal igs-rflp, phylogenies of 16s rrna and housekeeping genes (atpd, reca and glnii) and dna-dna relatedness. three strains in group i were classified as r. giardinii, since they showed high gene sequence similarities (>97%) and dna-dna relatedness (64.3-67.5%) with r. giardinii h152t. groups ii, iii and i ... | 2010 | 20833881 |
| rhizobium giardinii is the microsymbiont of illinois bundleflower (desmanthus illinoensis (michx.) macmillan) in midwestern prairies. | illinois bundleflower (desmanthus illinoensis (michx.) macmillan) has potential as a grain and forage legume for the american midwest. inoculant-quality rhizobia for this legume have been identified but not previously characterized. rhizobia trapped from 20 soils in the natural range of the illinois bundleflower had characteristics that placed them overwhelmingly within the species rhizobium giardinii, one of the few occasions this species has been recovered from legumes, raising questions on th ... | 2006 | 17110983 |
| phylogenetic analysis of the genera bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium on the basis of 16s rrna gene and internally transcribed spacer region sequences. | a total of 128 strains was isolated from more than 23 legume hosts in korea. phylogenetic relationships between these korean isolates and reference strains of the genera bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were analysed using their 16s rrna gene and internally transcribed spacer (its) region sequences. among the bradyrhizobium strains, dendrograms based on both the 16s rrna gene and its region sequences produced two main groups. the its tree yielded at least two new cluste ... | 2005 | 15653885 |
| ethiopian soils harbor natural populations of rhizobia that form symbioses with common bean ( phaseolus vulgaris l.). | the diversity and taxonomic relationships of 83 bean-nodulating rhizobia indigenous to ethiopian soils were characterized by pcr-rflp of the internally transcribed spacer (its) region between the 16s and 23s rrna genes, 16s rrna gene sequence analysis, multilocus enzyme electrophoresis (mlee), and amplified fragment-length polymorphism. the isolates fell into 13 distinct genotypes according to pcr-rflp analysis of the its region. based on mlee, the majority of these genotypes (70%) was genetical ... | 2004 | 14685648 |