| genetic diversity of rhizobial symbionts isolated from legume species within the genera astragalus, oxytropis, and onobrychis. | the genetic diversity of 44 rhizobial isolates from astragalus, oxytropis, and onobrychis spp. originating from different geographic locations was evaluated by mapped restriction site polymorphism (mrsp) analysis of 16s rrna genes and by pcr dna fingerprinting with repetitive sequences (rep-pcr). a comparison of tree topologies of reference strains constructed with data obtained by mrsp and by 16s rrna gene sequence analyses showed that the topologies were in good agreement, indicating that the ... | 1997 | 9406393 |
| conservation of plasmid-encoded traits among bean-nodulating rhizobium species. | rhizobium etli type strain cfn42 contains six plasmids. we analyzed the distribution of genetic markers from some of these plasmids in bean-nodulating strains belonging to different species (rhizobium etli, rhizobium gallicum, rhizobium giardinii, rhizobium leguminosarum, and sinorhizobium fredii). our results indicate that independent of geographic origin, r. etli strains usually share not only the psym plasmid but also other plasmids containing symbiosis-related genes, with a similar organizat ... | 2002 | 11976134 |
| the genetic diversity of rhizobia associated with dalea purpurea vent. in fragmented grasslands of west-central minnesota. | the increase in human population and the spread of agriculture over the past 150 years have transformed the landscape in west-central minnesota into a mosaic of agricultural fields and urban land, leaving only remnants of the once dominant prairie ecosystem. limited natural habitat in this fragmented landscape threatens the diversity and abundance of native legumes and could impact the size and function of associated belowground microbial populations. in this study, boxa1r pcr and 16s rrna gene ... | 2007 | 17538644 |
| characterization of phenol degradation by rhizobium sp. ccnwtb 701 isolated from astragalus chrysopteru in mining tailing region. | to screen high strength phenol degrading bacteria, we selected 108 rhizobial strains isolated from nodules of eight wild legumes species in the mining tailing region of shaanxi province, northwest of china, and cultivated them in a basal salt (bs) medium supplemented with different phenol concentrations as a sole carbon source. the results showed that some of the strains could use phenol as sole carbon source. in order to study the characteristics of phenol degradation, the strain ccnwtb701 isol ... | 2008 | 17624668 |
| 1-aminocyclopropane-1-carboxylate (acc) deaminase genes in rhizobia from southern saskatchewan. | a collection of 233 rhizobia strains from 30 different sites across saskatchewan, canada was assayed for 1-aminocyclopropane-1-carboxylate (acc) deaminase activity, with 27 of the strains displaying activity. when all 27 strains were characterized based on 16s rrna gene sequences, it was noted that 26 strains are close to rhizobium leguminosarum and one strain is close to rhizobium gallicum. polymerase chain reaction (pcr) was used to rapidly isolate acc deaminase structural genes from the above ... | 2009 | 18548183 |
| phenotypic and genotypic characterization of rhizobia associated with acacia saligna (labill.) wendl. in nurseries from algeria. | twenty seven rhizobial strains associated with acacia saligna grown in northern and southern algeria were characterized, including generation time, host-range, the 16s rrna gene and 16s-23s rrna intergenic spacer restriction patterns, 16s rrna gene sequence analysis and tolerance to salinity and drought. cross inoculation tests indicated that 11 slow-growing isolates from northern nurseries were able to nodulate introduced australian acacias exclusively, whereas 16 fast-growing isolates, mainly ... | 2010 | 19836181 |
| phylogenetic diversity of ammopiptanthus rhizobia and distribution of rhizobia associated with ammopiptanthus mongolicus in diverse regions of northwest china. | aiming to investigate the diversity and distribution of rhizobia associated with ammopiptanthus, an endangered evergreen legume widely distributed in deserts, we characterized a total of 219 nodule isolates from nine sampling sites in northwest china with different soil characteristics based upon restriction fragment length polymorphism (rflp) analysis of 16s ribosomal rna (rrna) and symbiotic genes (nodc and nifh). ten isolates representing different 16s rrna-rflp types were selected for furthe ... | 2016 | 27079453 |
| sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (phaseolus vulgaris l.) in a northern tunisian field. | a total of 40 symbiotic bacterial strains isolated from root nodules of common bean grown in a soil located in the north of tunisia were characterized by pcr-rflp of the 16s rrna genes. six different ribotypes were revealed. nine representative isolates were submitted to phylogenetic analyses of rrs, reca, atpd, dnak, nifh and noda genes. the strains 23c40 and 23c95 representing the most abundant ribotype were closely related to sinorhizobium americanum cfnei 156(t). s. americanum was isolated f ... | 2012 | 22633818 |
| Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China. | A total of 159 endophytic bacteria were isolated from surface-sterilized root nodules of wild perennial Glycyrrhiza legumes growing on 40 sites in central and northwestern China. Amplified fragment length polymorphism (AFLP) genomic fingerprinting and sequencing of partial 16S rRNA genes revealed that the collection mainly consisted of Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium and Paenibacillus species. Based on symbiotic properties with the legume hosts Glycyrrhiza uralensis and Gl ... | 2011 | 22066910 |
| characterization of rhizobia from legumes of agronomic interest grown in semi-arid areas of central spain relates genetic differences to soil properties. | a study of symbiotic bacteria from traditional agricultural legumes from central spain was performed to create a collection of rhizobia from soils differing in physicochemical, analytical and/or agroecological properties which could be well-adapted to the environmental conditions of this region, and be used for sustainable agricultural practices. thirty-six isolates were obtained from root-nodules of fifteen legume species (including cicer arietinum, lathyrus sativus, lens culinaris, lupinus spp ... | 2011 | 21953333 |
| microbial degradation of microcystin in florida's freshwaters. | presence of microcystin (mc), a predominant freshwater algal toxin and a suspected liver carcinogen, in florida's freshwaters poses serious health threat to humans and aquatic species. being recalcitrant to conventional physical and chemical water treatment methods, biological methods of mc removal is widely researched. water samples collected from five sites of lake okeechobee (lo) frequently exposed to toxic microcystis blooms were used as inoculum for enrichment with microcystin lr (mc-lr) su ... | 2011 | 21611743 |
| different species and symbiotic genotypes of field rhizobia can nodulate phaseolus vulgaris in tunisian soils. | abstract a collection of 160 isolates of rhizobia nodulating phaseolus vulgaris in three geographical regions in tunisia was characterized by restriction fragment length polymorphism analysis of polymerase chain reaction (pcr)-amplified 16s rdna, nifh and nodc genes. nine groups of rhizobia were delineated: rhizobium gallicum biovar (bv.) gallicum, rhizobium leguminosarum bv. phaseoli and bv. viciae, rhizobium etli bv. phaseoli, rhizobium giardinii bv. giardinii, and four groups related to speci ... | 2002 | 19709241 |
| rhizobia with different symbiotic efficiencies nodulate acaciella angustissima in mexico, including sinorhizobium chiapanecum sp. nov. which has common symbiotic genes with sinorhizobium mexicanum. | bacteria from nodules of the legume acaciella angustissima native to the south of mexico were characterized genetically and their nodulation and competitiveness were evaluated. phylogenetic studies derived from rpob gene sequences indicated that a. angustissima is nodulated by sinorhizobium mexicanum, rhizobium tropici, mesorhizobium plurifarium and agrobacterium tumefaciens and by bacteria related to sinorhizobium americanum, sinorhizobium terangae, rhizobium etli and rhizobium gallicum. a new ... | 2009 | 19120461 |
| genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina). | a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ... | 2009 | 19074602 |
| diverse bacteria isolated from root nodules of wild vicia species grown in temperate region of china. | in the present study, a total of 154 bacterial strains isolated from nodules of eighteen vicia species mainly grown in the temperate chinese provinces were characterized by ardra, its pcr-rflp, box-pcr, sequencing of 16s rdna, nodc, nifh, atpd and glnii, and nodulation tests. the results demonstrated that most of the r. leguminosarum strains were effective microsymbionts of the wild vicia species, while genomic species related to rhizobium gallicum, mesorhizobium huakuii, ensifer meliloti and br ... | 2008 | 18704366 |
| mesorhizobium albiziae sp. nov., a novel bacterium that nodulates albizia kalkora in a subtropical region of china. | a novel mesorhizobium group associated with albizia kalkora [wang et al. (2006), syst appl microbiol 29, 502-517] was further characterized. the seven strains in this group showed similar protein patterns and were different from defined mesorhizobium species in sds-page of whole-cell proteins. the representative strain ccbau 61158(t) formed a novel mesorhizobium lineage in phylogenetic analyses of 16s rrna, atpd, glnii and nifh genes. however, its nodc gene sequence was more similar to that of r ... | 2007 | 17551028 |
| diversity and geographical distribution of rhizobia associated with lespedeza spp. in temperate and subtropical regions of china. | eighty-eight root-nodule isolates from lespedeza spp. grown in temperate and subtropical regions of china were characterized by a polyphasic approach. nine clusters were defined in numerical taxonomy and sds-page analysis of whole cell proteins. based upon further characterizations of amplified 16s rdna restriction analysis (ardra), pcr-based restriction fragment length polymorphism of ribosomal igs, 16s rdna sequence analysis and dna-dna hybridization, these isolates were identified as bradyrhi ... | 2007 | 17530227 |
| salt-tolerant rhizobia isolated from a tunisian oasis that are highly effective for symbiotic n2-fixation with phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of sinorhizobium meliloti. | nodulation of common bean was explored in six oases in the south of tunisia. nineteen isolates were characterized by pcr-rflp of 16s rdna. three species of rhizobia were identified, rhizobium etli, rhizobium gallicum and sinorhizobium meliloti. the diversity of the symbiotic genes was then assessed by pcr-rflp of nodc and nifh genes. the majority of the symbiotic genotypes were conserved between oases and other soils of the north of the country. sinorhizobia isolated from bean were then compared ... | 2007 | 17019605 |
| agrobacterium strains isolated from root nodules of common bean specifically reduce nodulation by rhizobium gallicum. | in a previous work, we showed that non-nodulating agrobacteria strains were able to colonize root nodules of common bean. both rhizobia and agrobacteria co-existed in the infected nodules. no impact on symbiosis was found in laboratory conditions when using sterile gravel as a support for growth. in this study, soil samples originating from different geographic and agronomic regions in tunisia were inoculated with a mixture of agrobacteria strains isolated previously from root nodules of common ... | 2006 | 16629759 |
| structural determination of the nod factors produced by rhizobium gallicum bv. gallicum r602. | rhizobium gallicum is a fast-growing bacterium found in european, australian and african soils; it was first isolated in france. it is a microsymbiont which is able to nodulate plants of the genus phaseolus. rhizobium gallicum bv. gallicum r602 produces four extracellular signal molecules consisting of a linear backbone of n-acetyl glucosamine, bearing on the nonreducing terminal residue an n-methyl group and different n-acyl substituents. the four acyloligosaccharides terminate with a sulfated ... | 2006 | 16436076 |
| evolutionary genetics and biogeographic structure of rhizobium gallicum sensu lato, a widely distributed bacterial symbiont of diverse legumes. | we used phylogenetic and population genetics approaches to evaluate the importance of the evolutionary forces on shaping the genetic structure of rhizobium gallicum and related species. we analysed 54 strains from several populations distributed in the northern hemisphere, using nucleotide sequences of three 'core' chromosomal genes (rrs, glnii and atpd) and two 'auxiliary' symbiotic genes (nifh and nodb) to elucidate the biogeographic history of the species and symbiotic ecotypes (biovarieties) ... | 2005 | 16262857 |
| characteristics of the rhizobia associated with dalea spp. in the ordway, kellogg-weaver dunes, and hayden prairies. | habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. in this paper, we consider the diversity of the rhizobia associated with the indigenous legume dalea purpurea in 3 residual prairie areas in minnesota and iowa. using dalea purpurea as a trap host, 218 rhizobia were recovered from these soils then characterized using boxa1r pcr. three major and 13 minor groups were distinguished b ... | 2005 | 15782230 |
| identification of fast-growing rhizobia nodulating tropical legumes from puerto rico as rhizobium gallicum and rhizobium tropici. | fifteen isolates from several nodulated tropical legumes from puerto rico (usa) were characterised by their phenotypic, molecular and symbiotic features. the identification of isolates was based on a polyphasic approach, including phenotypic characteristics, 16s rrna sequencing, low molecular weight (lmw) rna profiles, two primers-rapd patterns, and restriction patterns from 16s rdna molecules. despite of the variety of hosts included in this study the 15 isolates were separated into only two gr ... | 2004 | 15368853 |
| diversity of 16s rdna sequences of rhizobium spp. implications for species determinations. | comparative analysis of 70 16s rdna sequences representing 20 rhizobium species (including pathogenic agrobacterium spp.) was conducted using maximum likelihood to establish relationships of species using multiple sequences. there is no significant internal division of the rhizobium clade to suggest that it represents more than one genus. plant pathogenic (agrobacterium) species are distributed within the genus. the analysis supported the synonymy of some species (rhizobium gallicum and rhizobiu ... | 2004 | 15336412 |
| ethiopian soils harbor natural populations of rhizobia that form symbioses with common bean ( phaseolus vulgaris l.). | the diversity and taxonomic relationships of 83 bean-nodulating rhizobia indigenous to ethiopian soils were characterized by pcr-rflp of the internally transcribed spacer (its) region between the 16s and 23s rrna genes, 16s rrna gene sequence analysis, multilocus enzyme electrophoresis (mlee), and amplified fragment-length polymorphism. the isolates fell into 13 distinct genotypes according to pcr-rflp analysis of the its region. based on mlee, the majority of these genotypes (70%) was genetical ... | 2004 | 14685648 |
| rhizobium etli and rhizobium gallicum nodulate common bean (phaseolus vulgaris) in a traditionally managed milpa plot in mexico: population genetics and biogeographic implications. | the stability of the genetic structure of rhizobial populations nodulating phaseolus vulgaris cultivated in a traditionally managed milpa plot in mexico was studied over three consecutive years. the set of molecular markers analyzed (including partial rrs, glnii, nifh, and nodb sequences), along with host range experiments, placed the isolates examined in rhizobium etli bv. phaseoli and rhizobium gallicum bv. gallicum. cluster analysis of multilocus enzyme electrophoresis and plasmid profile dat ... | 2003 | 12571008 |
| rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in china. | a novel rhizobial group, cluster 9, defined in previous research [tan, z. y., wang, e. t., peng, g. x., zhu, m. e., martinez-romero, e. & chen, w. x. (1999). int j syst bacteriol 49, 1457-1469], was further characterized by determination of dna base composition, whole-cell protein sds-page analysis, dna-dna hybridization, 16s rrna gene sequencing and host specificity. these isolates were collected from the wild legumes amphicarpaea trisperma, coronilla varia and gueldenstaedtia multiflora growin ... | 2001 | 11411714 |
| characterization of rhizobial isolates of phaseolus vulgaris by staircase electrophoresis of low-molecular-weight rna. | low-molecular-weight (lmw) rna molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in spain. since lmw rna profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from spanish soils presumptively could be characterized as rhizobium etli, rhizobium gallicum, rhizobium giardinii, rhizobium leguminosarum bv. viciae and bv. trifolii, and sinorhizobium fredii. | 2001 | 11157280 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| classification of austrian rhizobia and the mexican isolate fl27 obtained from phaseolus vulgaris l. as rhizobium gallicum. | the phylogenetic positions of four rhizobial strains obtained from nodules of common bean plants (phaseolus vulgaris l.) grown in an austrian soil and of the mexican bean isolate fl27 are described. analysis of the 16s rrna genes revealed sequences almost identical to that of the rhizobium gallicum type strain, r602sp, with a maximum of two nucleotide substitutions. comparison of the 16s rrna gene sequences with those from other bacteria indicated highest similarity to rhizobium sp. strain ok-50 ... | 1997 | 9336913 |
| rhizobium gallicum sp. nov. and rhizobium giardinii sp. nov., from phaseolus vulgaris nodules. | thirty-one strains of two new genomic species (genomic species 1 and 2) of rhizobia isolated from root nodules of phaseolus vulgaris and originating from various locations in france were compared with reference strains of rhizobia by performing a numerical analysis of 64 phenotypic features. each genomic species formed a distinct phenon and was separated from the other rhizobial species. a comparison of the complete 16s rrna gene sequences of a representative of genomic species 1 (strain r602spt ... | 1997 | 9336898 |
| rhizobium halotolerans sp. nov., isolated from chloroethylenes contaminated soil. | the strain designated as ab21(t) was isolated from chloroethylenes contaminated soil. cells are gram-negative, aerobic, non-spore-forming, and motile rods. phylogenetic analysis based on 16s rrna gene sequence showed that it belonged to the genus rhizobium, and was closely related to rhizobium sullae is 123(t) (97.4 %), rhizobium yanglingense sh 22623(t) (97.2 %), rhizobium gallicum r 602sp(t) (97.1 %), rhizobium alamii gbv 016(t) (97.0 %), and rhizobium monogolense usda 1844(t) (97.0 %). it sho ... | 2013 | 23377488 |
| complete genome sequences of three rhizobium gallicum symbionts associated with common bean (phaseolus vulgaris). | the whole-genome sequences of three strains of rhizobium gallicum reported here support the concept that the distinct nodulation host ranges displayed by the symbiovars gallicum and phaseoli can be largely explained by different symbiotic plasmids. | 2017 | 28302777 |
| genomic and phenotypic characterization of rhizobium gallicum phage vb_rgls_p106b. | the phage p106b (vb_rgls_p106b) is a siphoviridae phage with a narrow spectrum of infectivity, which has been isolated from soils with a history of pea cultivation. the trapping host of p106b is an indigenous strain of rhizobium gallicum (so14b-4) isolated from soils associated with vicia cracca. phenotypic characterization of the phage revealed that p106b has an approximate burst size of 21 p.f.u. per infected cell with 60 min and 100 min eclipse and latent periods, respectively. phage p106b wa ... | 2015 | 25627439 |
| rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of phaseolus vulgaris. | three microbial strains isolated from common beans, 23c2t (tunisia), gr42 (spain) and ie4868 (mexico), which have been identified previously as representing a genomic group closely related to rhizobium gallicum, are further studied here. their 16s rrna genes showed 98.5-99% similarity with rhizobium loessense ccbau 7190bt, r. gallicum r602spt, rhizobium mongolense usda 1844t and rhizobium yanglingense ccbau 71623t. phylogenetic analysis based on reca, atpd, dnak and thrc sequences showed that th ... | 2014 | 24478208 |
| diverse nodule bacteria were associated with astragalus species in arid region of northwestern china. | the legume species of astragalus as traditional chinese medicine source and environmental protection plants showed an extensive distribution in the arid region of northwestern china. however, few rhizobia associating with astragalus have been investigated in this region so far. in this study, 78 endophytic bacteria were isolated from root nodules of 12 astragalus species and characterized by the pcr-rflp of 16s rrna gene and symbiotic genes together with the phylogenetic analysis. results showed ... | 2015 | 24115208 |