| a strain of fusarium kyushuense is able to produce aflatoxin b1 and g 1. | a strain of f. kyushuense is able to produce aflatoxin b1 and g1 under certain growth conditions. this was ultimately demonstrated by lc-ms analysis. by using a microarray covering oligonucleotides specific for the aflatoxin pathway genes of a. flavus, the expression of homologues of nearly the complete aflatoxin biosynthesis gene cluster was detected in f. kyushuense under producing conditions, e.g., after growth in liquid yes medium. in contrast, after growth of f. kyushuense on solid yes medi ... | 2009 | 23605093 |
| metabolic effects of azoxystrobin and kresoxim-methyl against fusarium kyushuense examined using the biolog ff microplate. | azoxystrobin and kresoxim-methyl are strobilurin fungicides, and are effective in controlling many plant diseases, including fusarium wilt. the mode of action of this kind of chemical is inhibition of respiration. this research investigated the sensitivities of fusarium kyushuense to azoxystrobin and kresoxim-methyl, and to the alternative oxidase inhibitor salicylhydroxamic acid (sham). the biolog ff microplate is designed to examine substrate utilization and metabolic profiling of micro-organi ... | 2016 | 27155484 |
| pentahydroxyscirpene-producing strains, formation in planta, and natural occurrence. | trichothecenes are a class of structurally diverse mycotoxins with more than 200 naturally occurring compounds. previously, a new compound, pentahydroxyscirpene (phs), was reported as a byproduct of a nivalenol producing fusarium strain, ifa189. phs contains a hydroxy group at c-8 instead of the keto group of type b trichothecenes. in this work, we demonstrate that ifa189 belongs to the species fusarium kyushuense using molecular tools. production of phs in vitro was also observed for several is ... | 0 | 27754401 |
| specific detection of fusarium langsethiae and related species by dgge and arms-pcr of a beta-tubulin (tub1) gene fragment. | fusarium langsethiae was recently described as a new toxigenic fusarium species, which morphologically resembles fusarium poae, but exhibits a mycotoxin pattern related to fusarium sporotrichioides. to develop tools for early and specific detection of f. langsethiae and distinguishing it from related species of section sporotrichiella and discolor (f. poae, f. sporotrichioides, fusarium kyushuense, fusarium robustum, fusarium sambucinum and fusarium tumidum) sequence variations in their beta-tub ... | 2004 | 15337597 |
| igs-rflp analysis and development of molecular markers for identification of fusarium poae, fusarium langsethiae, fusarium sporotrichioides and fusarium kyushuense. | the intergenic spacer (igs) regions of the rdna of several fusarium spp. strains obtained from the collaborative researchers (int. j. food microbiol. (2003)) were amplified by polymerase chain reaction (pcr), and an igs-rflp analysis was performed. restriction digestion with alui, mspi and psti allowed differentiation between the related fusarium poae and fusarium kyushuense species. fusarium langsethiae was also separated from fusarium sporotrichioides (including var. minus) on the basis of the ... | 2004 | 15337596 |
| the use of tri5 gene sequences for pcr detection and taxonomy of trichothecene-producing species in the fusarium section sporotrichiella. | purified dna from isolates of fusarium poae, fusarium sporotrichioides, fusarium kyushuense and fusarium langsethiae was used as a template to amplify a 658-bp fragment from the trichodiene synthase (tri5) gene of these fungi with the gene-specific pcr primer pair tox5-1/tox5-2. fragments obtained were isolated and sequenced. dna sequence alignments revealed high similarity between the sequences derived from f. sporotrichioides and f. langsethiae (98.7%) and less similarity between the latter sp ... | 2004 | 15337595 |
| phylogenetic analyses of the fusarium poae, fusarium sporotrichioides and fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene. | phylogenetic relationships between four fusarium species were studied using parts of the nuclear translation elongation factor-1 alpha (ef-1alpha) gene as a phylogenetic marker. sequences from 12 isolates of fusarium poae, 10 isolates of fusarium sporotrichioides and 12 isolates of fusarium langsethiae yielded 4, 5 and 5 haplotypes, respectively. in addition, we included one isolate of fusarium kyushuense. the aligned sequences were subjected to neighbor-joining (nj), maximum parsimony and maxim ... | 2004 | 15337593 |
| phylogenetic relationship of fusarium langsethiae to fusarium poae and fusarium sporotrichioides as inferred by igs, its, beta-tubulin sequences and up-pcr hybridization analysis. | fusarium langsethiae was recently described to accommodate "powdery" isolates of fusarium poae, which morphologically resemble f. poae, but whose metabolite profile is similar to that of fusarium sporotrichioides. in order to investigate the phylogenetic relationship of f. langsethiae to closely related species, we sequenced the internal transcribed spacer (its) regions 1 and 2 and part of the intergenic spacer (igs) region of the rdna cluster and part of the beta-tubulin gene from 109 strains o ... | 2004 | 15337592 |
| expression of inducible nitric oxide synthase and enzymes of arginine metabolism in fusarium kyushuense-exposed mouse lung. | expression of inducible nitric oxide (no) synthase (inos) and related enzymes of arginine metabolism in the mouse lung exposed to filamentous fungus fusarium kyushuense was studied by rna blot, immunoblot, and histological analyses. when mice were exposed intranasally to the fungi only once, no induction of inos mrna was observed. however, when the animals were infected again 6 days after the first exposure, inos mrna was induced, reached a maximum 12-24 h after the exposure, and decreased to an ... | 1999 | 10444369 |