| agrobacterium tumefaciens-mediated transformation of filamentous fungi. | agrobacterium tumefaciens transfers part of its ti plasmid, the t-dna, to plant cells during tumorigenesis. it is routinely used for the genetic modification of a wide range of plant species. we report that a. tumefaciens can also transfer its t-dna efficiently to the filamentous fungus aspergillus awamori, demonstrating dna transfer between a prokaryote and a filamentous fungus. we transformed both protoplasts and conidia with frequencies that were improved up to 600-fold as compared with conve ... | 1998 | 9743116 |
| combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in fusarium venenatum. | fusarium venenatum jers 325, a strain which produces recombinant glucoamylase under control of a growth rate independent promoter was transformed with a plasmid carrying the aspergillus niger glucoamylase gene under control of its own growth rate correlated promoter. some disruption of the original recombinant genes occurred and at ph 5.8 the double transformant did not produce as much glucoamylase as jers 325 in batch culture. however, the double transformant still produced as much glucoamylase ... | 2001 | 11164313 |
| siderophore production by fusarium venenatum a3/5. | fusarium venenatum a3/5 was grown in iron-restricted batch cultures and iron-limited chemostat cultures to determine how environmental conditions affected siderophore production. the specific growth rate in iron-restricted batch cultures was 0.22 h(-1), which was reduced to 0.12 h(-1) when no iron was added to the culture. d(crit) in iron-limited chemostat culture was 0.1 h(-1). siderophore production was correlated with specific growth rate, with the highest siderophore production occurring at ... | 2002 | 12196167 |
| sensitivity to quorn mycoprotein (fusarium venenatum) in a mould allergic patient. | | 2002 | 12401831 |
| evaluation of certain food additives. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents), assessments of intake, and the establishment and revision of spe ... | 2006 | 17069402 |
| myco-protein from fusarium venenatum: a well-established product for human consumption. | fusarium venenatum a3/5 was first chosen for development as a myco-protein in the late 1960s. it was intended as a protein source for humans and after 12 years of intensive testing, f. venenatum a3/5 was approved for sale as food by the ministry of agriculture, fisheries and food in the united kingdom in 1984. today, myco-protein is produced in two 150,000 l pressure-cycle fermenters in a continuous process which outputs around 300 kg biomass/h. the continuous process is typically operated for a ... | 2002 | 11954786 |
| evolution of a recombinant (gucoamylase-producing) strain of fusarium venenatum a3/5 in chemostat culture. | fusarium venenatum jers 325 is a transformant of strain a3/5 which produces aspergillus niger glucoamylase (gam) under the control of a fusarium oxysporum trypsin-like protease promoter. the evolution of jers 325 was studied in glucose-limited chemostat cultures grown on nano3 or (nh4)2so4 as the nitrogen source. thirteen mutants which were more highly branched and four mutants which were more sparsely branched than the parental strain were isolated from the nano3 chemostat. the highly branched ... | 2001 | 11255162 |
| toxicological studies on thermomyces lanuginosus xylanase expressed by fusarium venenatum, intended for use in food. | the xylanase used in this study was produced by a submerged fermentation of fusarium venenatum and contained a gene code originating from thermomyces lanuginosus. the enzyme was subject to a 13-week toxicological test in rats and in vitro tests to document its safety in use. the enzyme is to be applied as a processing aid in the baking industry to improve handling and stability of dough. the enzyme was not found to be mutagenic in the salmonella typhimurium reverse mutation assay, nor did it cau ... | 2000 | 11091786 |
| a non-specific aminopeptidase from aspergillus. | a fermentation broth supernatant of the aspergillus oryzae strain atcc20386 contains aminopeptidase activity that releases a wide variety of amino acids from natural peptides. the supernatant was fractionated by anion exchange chromatography. based on the primary amino acid sequence data obtained from proteins in certain fractions, polymerase chain reaction (pcr) primers were made and a pcr product was generated. this pcr product was used to screen an a. oryzae cdna library from which the full l ... | 2000 | 10899618 |
| growth-rate-independent production of recombinant glucoamylase by fusarium venenatum jers 325. | most recombinant proteins generated in filamentous fungi are produced in fed-batch cultures, in which specific growth rate normally decreases progressively with time. because of this, such cultures are more suited to the production of products that are produced efficiently at low-growth rates (e.g., penicillin) than to products which are produced more efficiently at high-growth rates (e. g., glucoamylase). fusarium venenatum a3/5 has been transformed (jers 325) to produce aspergillus niger gluco ... | 2000 | 10745192 |
| deletion of the trichodiene synthase gene of fusarium venenatum: two systems for repeated gene deletions. | the trichodiene synthase (tri5) gene of fusarium venenatum was cloned from a genomic library. vectors were created in which the tri5 coding sequence was replaced with the neurospora crassa nitrate reductase (nit3) gene and with the aspergillus nidulans acetamidase (amds) gene flanked by direct repeats. the first vector was utilized to transform a nitrate reductase (niad) mutant of f. venenatum to prototrophy, and the second vector was utilized to confer acetamide utilization to the wild-type str ... | 1999 | 10512673 |
| purification, characterization, and heterologous expression in fusarium venenatum of a novel serine carboxypeptidase from aspergillus oryzae. | a novel serine carboxypeptidase (ec 3.4.16.1) was found in an aspergillus oryzae fermentation broth and was purified to homogeneity. this enzyme has a molecular weight of ca. 67,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity is 21 u/mg for carbobenzoxy (z)-ala-glu at ph 4.5 and 25 degrees c. it has a ratio of bimolecular constants for z-ala-lys and z-ala-phe of 3.75. optimal enzyme activity occurs at ph 4 to 4.5 and 58 to 60 degrees c f ... | 1999 | 10427010 |
| ph regulation of recombinant glucoamylase production in fusarium venenatum jers 325, a transformant with a fusarium oxysporum alkaline (trypsin-like) protease promoter. | fusarium venenatum (formerly fusarium graminearum) jers 325 produces heterologous glucoamylase (gam) under the regulation of a fusarium oxysporum alkaline (trypsin-like) protease promoter. the glucoamylase gene was used as a reporter gene to study the effects of ammonium and ph on gam production under the control of the alkaline protease promoter. between ph 4.0 and 5.8, gam production in glucose-limited chemostat cultures of jers 325 grown at a dilution rate of 0.10 h-1 (doubling time, 6.9 h) o ... | 1999 | 10397874 |
| molecular phylogenetic, morphological, and mycotoxin data support reidentification of the quorn mycoprotein fungus as fusarium venenatum. | | 1998 | 9806808 |
| molecular characterization and expression of a phytase gene from the thermophilic fungus thermomyces lanuginosus. | the phya gene encoding an extracellular phytase from the thermophilic fungus thermomyces lanuginosus was cloned and heterologously expressed, and the recombinant gene product was biochemically characterized. the phya gene encodes a primary translation product (phya) of 475 amino acids (aa) which includes a putative signal peptide (23 aa) and propeptide (10 aa). the deduced amino acid sequence of phya has limited sequence identity (ca. 47%) with aspergillus niger phytase. the phya gene was insert ... | 1998 | 9797301 |
| molecular phylogenetic, morphological, and mycotoxin data support reidentification of the quorn mycoprotein fungus as fusarium venenatum. | molecular phylogenetic, morphological, and mycotoxin data were obtained in order to investigate the relationships and identity of the quorn mycoprotein fungus within fusarium and to examine quorn strains and commercial quorn food products for trichothecene mycotoxins. phylogenetic analyses of aligned dna sequences obtained via the polymerase chain reaction from the nuclear 28s ribosomal dna, nuclear ribosomal internal transcribed spacer region, and beta-tubulin gene exons and introns indicate th ... | 1998 | 9501477 |
| chemical and physiological characterization of taxa in the fusarium sambucinum complex. | forty-one isolates of fusarium sambucinum sensu lato were screened for production of secondary metabolites in agar cultures. of 16 strains of f. sambucinum sensu stricto all but two strains produced diacetoxyscirpenol and two unidentified metabolites, tb1 and tb2 respectively. the two remaining f. sambucinum strains produced t-2 toxin, tb1 and tb2. fusarium venenotum (6 strains) produced diacetoxyscirpenol and an unidentified metabolite bb. fusarium torulosum (8 strains) produced wortmannin and ... | 1995 | 7566056 |
| employing central composite design for evaluation of biomass production by fusarium venenatum: in vivo antioxidant and antihyperlipidemic properties. | the present study deals with the cost effective production of biomass from fusarium venenatum using different carbon sources (cane sugar, brown sugar, malt and fructose). optimization of selected carbon sources and seed size using central composite response surface design (ccrsd) indicated that sucrose (1.64 g/100 ml) and seed size (10% v/v) were optimal in maximizing biomass yield (0.5602 g/100 ml, p < 0.0001) and protein yield (49.99%, p < 0.01) of fusarium venenatum. the acetonitrile and meth ... | 2017 | 28194718 |
| utilizing pretreatment and fungal incubation to enhance the nutritional value of canola meal. | the objective of this study was to determine the optimal pretreatment and fungal strain to reduce glucosinolates (gls), fibre and residual sugars while increasing the nutritional value of canola meal. | 2017 | 28703403 |
| screening survey of co-production of fusaric acid, fusarin c, and fumonisins b₁, b₂ and b₃ by fusarium strains grown in maize grains. | fusarium species isolated from belgian maize were screened for their ability to produce fusarin c, fusaric acid, fumonisins b1 (fb1), fb2 and fb3 in maize grains. first, cultivation of fusarium species in myro liquid medium allowed overcoming the shortage of the standard of fusarin c on the market. all fusarium verticillioides produced much higher contents of mycotoxins in myro compared to fusarium graminearum or fusarium venenatum. the optimization of the lc-ms/ms method resulted in low limits ... | 2014 | 25270005 |
| engineered production of fungal anticancer cyclooligomer depsipeptides in saccharomyces cerevisiae. | two fungal cyclooligomer depsipeptide synthetases(codss), bbbeas (352 kda) and bbbsls (348 kda) from beauveria bassiana atcc7159, were reconstituted in saccharomyces cerevisiae bj5464-npga, leading to the production of the corresponding anticancer natural products, beauvericins and bassianolide, respectively. the titers of beauvericins (33.8 ± 1.4 mg/l) and bassianolide (21.7± 0.1 mg/l) in the engineered s. cerevisiae bj5464-npga strains were comparable to those in the native producer b. bassian ... | 2013 | 23608474 |
| evaluation of certain food additives. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to concluding as to safety concerns and to preparing specifications for identity and purity. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of and assessment of dietary exposure to food additives, including flavouring agents. a summary follows of the commi ... | 2012 | 23600165 |
| evaluation of certain food additives and contaminants. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to recommending acceptable daily intakes (adis) and to preparing specifications for identity and purity. the committee also evaluated the risk posed by two food contaminants, with the aim of advising on risk management options for the purpose of public health protection. the first part of the report contains a general discu ... | 2007 | 18551832 |
| production of fusarium solani f. sp. pisi cutinase in fusarium venenatum a3/5. | fusarium venenatum a3/5 was transformed using the aspergillus niger expression plasmid, pigf, in which the coding sequence for the f. solani f. sp. pisi cutinase gene had been inserted in frame, with a kex2 cleavage site, with the truncated a. niger glucoamylase gene under control of the a. niger glucoamylase promoter. the transformant produced up to 21 u cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. glucoamylase (165 u l(-1) or 8 mg l(-1)) was also ... | 2007 | 17505784 |
| modification of milk and whey surface properties by enzymatic hydrolysis of milk phospholipids. | phospholipase a1 were shown to improve foaming properties of skim milk and whey, implying that phospholipases can be useful tools for modifying the functionality of dairy products and ingredients. the ability of three fungal phospholipases and porcine pancreatic phospholipase a2 to hydrolyze milk phospholipids was investigated by using sodium deoxycholate-solubilized milk phospholipid and whole milk. the enzyme with the highest activity in milk was fusarium venenatum phospholipase a1. milk and w ... | 2007 | 17373808 |
| fusarin c biosynthesis in fusarium moniliforme and fusarium venenatum. | fragments of polyketide synthase (pks) genes were amplified from complementary dna (cdna) of the fusarin c producing filamentous fungi fusarium moniliforme and fusarium venenatum by using degenerate oligonucleotides designed to select for fungal pks c-methyltransferase (cmet) domains. the pcr products, which were highly homologous to fragments of known fungal pks cmet domains, were used to probe cdna and genomic dna (gdna) libraries of f. moniliforme and f. venenatum. a 4.0 kb cdna clone from f. ... | 2004 | 15368570 |
| evaluation of certain food additives and contaminants. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents) and contaminants, assessments of intake, and the establishment an ... | 2004 | 15354533 |
| toxicological studies on lactose oxidase from microdochium nivale expressed in fusarium venenatum. | a new carbohydrate oxidase, lactose oxidase, with high specificity of oxidizing the disaccharide lactose to lactobionic acid has been found. this enzyme opens up for a variety of applications. a programme of toxicological studies was conducted to establish the safety of lactose oxidase to be used as a processing aid in the food industry. the enzyme used in this study was produced by a submerged fermentation of fusarium venenatum and contained a gene code from microdochium nivale. oral administra ... | 2004 | 15135207 |
| immediate-type hypersensitivity reaction to ingestion of mycoprotein (quorn) in a patient allergic to molds caused by acidic ribosomal protein p2. | quorn is the brand name for a line of foods made with so-called "mycoprotein," which springs from the mold fusarium venenatum. since the introduction on the food market, there have been complaints from consumers reporting adverse gastrointestinal reactions after ingestion of mycoprotein. to date, it is not clear whether the reported symptoms are ige-mediated. | 2003 | 12743577 |