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[comparative study of the fractional protein composition of healthy and xanthomonas vesicatoria and corynebacterium michiganense infected tomato leaves]. 1977865315
[catalase isozyme spectrum of healthy tomato leaves and those inoculated with xanthomonas vesicatoria and corynebacterium michiganense]. 1977865321
development of a diagnostic dna probe for xanthomonads causing bacterial spot of peppers and tomatoes.xanthomonas vesicatoria and xanthomonas axonopodis pv. vesicatoria, causal agents for bacterial spot of tomatoes and peppers, are difficult to distinguish from other xanthomonads found on field-grown plants. a genomic subtraction technique with subtracter dna from nonpathogenic epiphytic xanthomonads was used to enrich for sequences that could serve as diagnostic probes for these pathogens. a 1.75-kb psti-noti fragment (kk1750) that preferentially hybridized to x. vesicatoria dna and x. axonopod ...19979361433
systematic analysis of xanthomonads (xanthomonas spp.) associated with pepper and tomato lesions.the taxonomy and evolutionary relationships among members of the genus xanthomonas associated with tomato and pepper have been a matter of considerable controversy since their original description in 1921. these bacteria, which are a major affliction of tomato and pepper crops in warm and humid regions, were originally described as a single species, but subsequent research has shown the existence of at least two genetic groups differentiated by physiological, biochemical and pathological charact ...200010843065
phylogenetic analysis of xanthomonas species based upon 16s-23s rdna intergenic spacer sequences.phylogenetic relationships of 17 species of xanthomonas were assessed based on analysis of 16s-23s rdna intergenic spacer (its) sequences; a higher level of resolution was obtained than that revealed by 16s rdna analysis. its sequences varied in size from 492 to 578 nt within the genus and the similarity among sequences ranged from 63 to 99%. major differences were found for the hyacinthi group, which included xanthomonas albilineans, xanthomonas hyacinthi, xanthomonas sacchari, xanthomonas thei ...200211931143
esterase este from xanthomonas vesicatoria ( xv_este) is an outer membrane protein capable of hydrolyzing long-chain polar esters.a new esterase gene from xanthomonas vesicatoria (formerly x. campestris) dsm 50861 was identified, cloned from a chromosomal gene library and overexpressed in escherichia coli. the corresponding dna fragment contains an orf of 1,818 bp, encoding a hydrolase of the gdsl esterase family. a protein of about 67 kda, named xv_este, was expressed from this fragment. a n-terminal signal peptide was processed under low-expression conditions, yielding a 63-kda mature protein. the predicted amino acid se ...200312764562
[certain biochemical changes in tomato shoots during artificial xanthomonas vesicatoria infection]. 195813600040
[changes in the intensity of respiration and activity of oxidizing enzymes in various resistant strains of tomatoes under the influence of xanthomonas vesicatoria infection]. 196013768352
[biology of the pathogen of bacterial black spots in tomatoes--xanthomonas vesicatoria. i. growth and toxin production by x. vesicatoria in some culture media]. 196114471478
isolation and antifungal and antioomycete activities of staurosporine from streptomyces roseoflavus strain ls-a24.the actinomycete strain ls-a24 active against some plant fungal and oomycete pathogens was isolated from a soil sample of the sunghwan lake in korea. the cell wall composition and spore shape of strain ls-a24 were ll-diaminopimelic acid and spiral type, respectively. on the basis of the physiological and biochemical characteristics and 16s ribosomal dna sequence analysis, strain ls-a24 was identical to streptomyces roseoflavus. an antifungal and antioomycete antibiotic was isolated from ls-a24 u ...200616608228
inhibition of plant-pathogenic bacteria by short synthetic cecropin a-melittin hybrid peptides.short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria erwinia amylovora, pseudomonas syringae, and xanthomonas vesicatoria and compared to the previously described peptide pep3 (wklfkkilkvl-nh(2)). the antimicrobial activity of pep3 and 22 analogues was evaluated in terms of the mic and the 50% effective dose (ed(50)) for growth. peptide cytotoxicity against human red blood cells and peptide stability toward protease degradation we ...200616672470
de novo designed cyclic cationic peptides as inhibitors of plant pathogenic bacteria.head-to-tail cyclic peptides of 4-10 residues consisting of alternating hydrophilic (lys) and hydrophobic (leu and phe) amino acids were synthesized and tested against the economically important plant pathogenic bacteria erwinia amylovora, xanthomonas vesicatoria and pseudomonas syringae. the antibacterial activity, evaluated as the minimal inhibitory concentration (mic), the cytotoxicity against human red blood cells and stability towards protease degradation were determined. the influence of c ...200616730857
improvement of cyclic decapeptides against plant pathogenic bacteria using a combinatorial chemistry approach.cyclic decapeptides were developed based on the previously reported peptide c(lysleulysleulysphelysleulysgln). these compounds were active against the economically important plant pathogenic bacteria erwinia amylovora, pseudomonas syringae and xanthomonas vesicatoria. a library of 56 cyclic decapeptides was prepared and screened for antibacterial activity and eukaryotic cytotoxicity, and led to the identification of peptides with improved minimum inhibitory concentration (mic) against p. syringa ...200616762457
antibacterial phenolic compounds from the spines of gleditsia sinensis lam.this study was to isolate antibacterial compounds from gleditsia sinensis lam. spines through bioassay-guided fractionation (against a gram-positive bacterium xanthomonas vesicatoria and a gram-negative bacterium bacillus subtilis). the crude ethanol extract of g. sinensis spines was partitioned sequentially with solvents of increasing polarity. the ethyl acetate fraction, which exhibited the most significant antibacterial activities among all the solvent fractions, was further separated by colu ...200717479415
fungal endophytes from dioscorea zingiberensis rhizomes and their antibacterial activity.the aim of the study was to isolate and characterize the endophytic fungi from the rhizomes of the chinese traditional medicinal plant dioscorea zingiberensis and to detect their antibacterial activities.200818086193
protein domain library generation by overlap extension (pdlgo): a tool for enzyme engineering.we introduce an upgraded version of the error-prone polymerase chain reaction (eppcr) comprising three dna polymerase-catalyzed steps. it improves the common eppcr strategy such that random mutations can be confined exactly to a distinct, but freely selectable, sequence region within a gene without the need for flanking restriction endonuclease sites. the new method is called protein domain library generation by overlap extension (pdlgo). to validate pdlgo, we generated a random library of este, ...200818452697
antimicrobial activity of globulol isolated from the fruits of eucalyptus globulus labill.an antimicrobial sesquiterpene was separated by bioassay-guided isolation from the petroleum ether fraction of the ethanol crude extract of eucalyptus globulus labill (myrtaceae) fruits, and was identified as globulol by physicochemical properties and spectroscopic analysis. mycelial growth method revealed that the median effective inhibitory concentration (ic50) values of globulol on alternaria solani, fusarium oxysporum f.sp. niverum, f. graminearum, rhizoctonia solani and venturia pirina were ...200818569693
antimicrobial phenolic compounds from anabasis aphylla l.bioassay-guided fractionation of an ethyl acetate extract from the aerial parts of anabasis aphylla, a chenopodiaceous species widely distributed in the northwest of china, led to the isolation of six phenolic compounds, which were identified by means of spectrometric analysis as 1-(2-hydroxy-4,6-dimethoxyphenyl)-ethanone (1), 3,4-dihydroxy cinnamic acid tetracosyl ester (2), 4-hydroxy-3-methoxy benzoic acid (3), 2-hydroxy benzoic acid (4), 3,4-dihydroxy cinnamic acid methyl ester (5) and 4-hydr ...200919413118
chemical composition and in vitro antimicrobial activity of the volatile oils from gliomastix murorum and pichia guilliermondii, two endophytic fungi in paris polyphylla var. yunnanensis.volatile oils were obtained by hydro-distillation from gliomastix murorum and pichia guilliermondii, two endophytic fungi isolated from the traditional chinese medicinal herb paris polyphylla var. yunnanensis. the oils were analyzed for their chemical composition by gas chromatography-mass spectrometry (gc-ms). palmitic acid (15.5%), (e)-9-octadecenoic acid (11.6%), 6-pentyl-5,6-dihydropyran-2-one (9.7%), and (7z,10z)-7,10- hexadecadienoic acid (8.3%) were the major compounds of the 40 identifie ...200919967981
beauvericin from the endophytic fungus, fusarium redolens, isolated from dioscorea zingiberensis and its antibacterial activity.from the fungal endophyte, fusarium redolens dzf2, isolated from rhizomes of the chinese medicinal plant dioscorea zingiberensis, beauvericin was obtained by tlc in combination with bioautographic antibacterial assay. the compound was identified by spectroscopic and physicochemical means. the median effective inhibitory concentration (ic50) values of beauvericin against six test bacteria (bacillus subtilis, staphylococcus haemolyticus, pseudomonas lachrymans, agrobacterium tumefaciens, escherich ...201020521553
antimicrobial flavonoids from the twigs of populus nigra × populus deltoides.a bioassay-guided fractionation of the ethyl acetate extract from the twigs of the hybrid poplar 'neva', populus nigra l. × populus deltoides marsh, led to the isolation of three flavonoids, which were identified by means of spectrometric and physicochemical analysis as 5-hydroxy-7-methoxy-flavone (1), 5,7-dihydoxy-flavone (2) and 5,7-dihydroxy-flavonol (3). these compounds were further screened for their antimicrobial activity against plant pathogens, including three bacteria (pseudomonas lachr ...201121416454
de-novo design of antimicrobial peptides for plant protection.this work describes the de-novo design of peptides that inhibit a broad range of plant pathogens. four structurally different groups of peptides were developed that differ in size and position of their charged and hydrophobic clusters and were assayed for their ability to inhibit bacterial growth and fungal spore germination. several peptides are highly active at concentrations between 0,1 and 1 µg/ml against plant pathogenic bacteria, such as pseudomonas syringae, pectobacterium carotovorum, an ...201323951222
functional analysis of the ferric uptake regulator gene fur in xanthomonas vesicatoria.iron is essential for the growth and survival of many organisms. intracellular iron homeostasis must be maintained for cell survival and protection against iron toxicity. the ferric uptake regulator protein (fur) regulates the high-affinity ferric uptake system in many bacteria. to investigate the function of the fur gene in xanthomonas vesicatoria (xv), we generated a fur mutant strain, fur-m, by site-directed mutagenesis. whereas siderophore production increased in the xv fur mutant, extracell ...201626910324
bioactive dibenzo-α-pyrone derivatives from the endophytic fungus rhizopycnis vagum nitaf22.six new dibenzo-α-pyrones, rhizopycnolides a (1) and b (2) and rhizopycnins a-d (3-6), together with eight known congeners (7-14), were isolated from the endophytic fungus rhizopycnis vagum nitaf22 obtained from nicotiana tabacum. the structures of the new compounds were unambiguously elucidated using nmr, hresims, tddft ecd calculation, and x-ray crystallography data. rhizopycnolides a (1) and b (2) feature an uncommon γ-butyrolactone-fused dibenzo-α-pyrone tetracyclic skeleton (6/6/6/5), while ...201627441892
bioactive spirobisnaphthalenes from the endophytic fungus berkleasmium sp.nine new spirobisnaphthalenes, palmarumycins b1-b9 (1-9), along with 13 known compounds (10-22), were isolated from cultures of the fungus berkleasmium sp., an endophyte isolated from the medicinal plant dioscorea zingiberensis c. h. wright. the structures of the new compounds were elucidated by analysis of the 1d and 2d nmr and hresims spectra and by comparison with known compounds. compounds 7-9 contain an uncommon 2,3-dihydro-1h-inden-1-one unit. all isolated compounds were evaluated for thei ...201425237727
screening for antimicrobial activity of wood rotting higher basidiomycetes mushrooms from uruguay against phytopathogens.in this work, the antimicrobial activity of extracts of wood rotting higher basidiomycetes mushrooms isolated from eucalyptus plantations in uruguay was studied using bacterial and fungal phytopathogens as targets. fifty-one extracts from mycelia and growth broth were prepared from higher basidiomycetes mushrooms, from which eight extracts (from ganoderma resinaceum, laetiporus sulphureus, dictyopanus pusillus, and bjerkandera adusta) showed antimicrobial activity against xanthomonas vesicatoria ...201627481160
high-antibacterial activity of urtica spp. seed extracts on food and plant pathogenic bacteria.the aim of this study was to comparatively evaluate antibacterial activities of methanol (metoh) and aqueous (dw) leaf (l), root (r) and seed (s) extracts of urtica dioica l. (ud; stinging nettle) and urtica pilulifera l. (up; roman nettle) on both food- and plant-borne pathogens, with total phenolic contents and dpph radical scavenging activities (drsa). metoh extracts of leaves and roots of u. dioica had the highest drsa. extracts with high antibacterial activity were in the order up-lmetoh (1 ...201323067263
dibenzo-α-pyrones: a new class of larvicidal metabolites against aedes aegypti from the endophytic fungus hyalodendriella sp. ponipodef12.in our search for new agrochemicals from endophytic fungi, the crude extract of the endophytic hyalodendriella sp. ponipodef12 associated with the hybrid 'neva' of populus deltoides marsh × p. nigra l. was found to possess larvicidal activity against aedes aegypti.201627862895
isolation and characterization of plant and human pathogenic bacteria from green pepper (capsicum annum l.) in riyadh, saudi arabia.forty-three bacterial isolates in five genera were recovered from naturally infected green pepper fruits (38 samples) showing dark brown, irregular-shaped splotches. the pathogenicity test was performed on healthy green pepper fruits and red colonies were from inoculated fruits showing the same symptoms and the infected area developed into soft rot. their identification was based on phenotypic characters and sequence of the gene fragment coding 16s rrna. of 43 isolates, 10 showing splotches on g ...201428324470
production of a de-novo designed antimicrobial peptide in nicotiana benthamiana.antimicrobial peptides are important defense compounds of higher organisms that can be used as therapeutic agents against bacterial and/or viral infections. we designed several antimicrobial peptides containing hydrophobic and positively charged clusters that are active against plant and human pathogens. especially peptide sp1-1 is highly active with a mic value of 0.1 μg/ml against xanthomonas vesicatoria, pseudomonas corrugata and pseudomonas syringae pv syringae. however, for commercial appli ...201323242916
complete genome sequences of six copper-resistant xanthomonas strains causing bacterial spot of solaneous plants, belonging to x. gardneri, x. euvesicatoria, and x. vesicatoria, using long-read technology.xanthomonas vesicatoria, xanthomonas euvesicatoria, and xanthomonas gardneri cause bacterial spot disease. copper has been applied since the 1920s as part of integrated management programs. the first copper-resistant strains were reported some decades later. here, we fully sequenced six xanthomonas strains pathogenic to tomato and/or pepper and having a copper-resistant phenotype.201728232425
bacterial spot of tomato and pepper: diverse xanthomonas species with a wide variety of virulence factors posing a worldwide challenge.bacteria; phylum proteobacteria; class gammaproteobacteria; order xanthomonadales; family xanthomonadaceae; genus xanthomonas; species xanthomonas euvesicatoria, xanthomonas vesicatoria, xanthomonas perforans and xanthomonas gardneri.201525649754
role of aromatic amino acids in lipopolysaccharide and membrane interactions of antimicrobial peptides for use in plant disease control.kye28 (kyeittihnlfrklthrlfrrnfgyt-lr), the representative sequence of helix d of heparin co-factor ii, was demonstrated to be potent against agronomically important gram-negative plant pathogens xanthomonas vesicatoria and xanthomonas oryzae, capable of inhibiting disease symptoms in detached tomato leaves. nmr studies in the presence of lipopolysaccharide provided structural insights into the mechanisms underlying this, notably in relationship to outer membrane permeabilization. the three-dimen ...201627137928
draft genome sequences of two xanthomonas vesicatoria strains from the balkan peninsula.xanthomonas vesicatoria causes bacterial spot disease of pepper and tomato plants. we report here the first genome sequences of x. vesicatoria strains that have been isolated from pepper plants. these data will be used for comparative genomics and will allow the development of new detection and typing tools for epidemiological surveillance.201525676765
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