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a new specific endonuclease present in xanthomonas holcicola, xanthomonas papavericola and brevibacterium luteum. 1978625054
isolation and partial characterization of bstvi, a thermostable isoschizomer of xhoi.a type ii restriction endonuclease, which has been named bstvi, was isolated and partially purified from a spore-forming, gram-positive thermophilic bacilli. on the basis of its digestion patterns on various dna's, it was concluded that this enzyme is an isoschizomer of xhoi, isolated originally from xanthomonas holcicola. besides being highly thermostable, the enzyme is produced in very large amounts by this bacterial strain. a single purification step renders it free of unspecific nucleases an ...19852992515
evaluation of the dna fingerprinting method aflp as an new tool in bacterial taxonomy.we investigated the usefulness of a novel dna fingerprinting technique, aflp, which is based on the selective amplification of genomic restriction fragments by pcr, to differentiate bacterial strains at the subgeneric level. in totals, 147 bacterial strains were subjected to aflp fingerprinting: 36 xanthomonas strains, including 23 pathovars of xanthomonas axonopodis and six pathovars of xanthomonas vasicola, one strain of stenotrophomonas, 90 genotypically characterized strains comprising all 1 ...19968757752
phylogenetic analysis of xanthomonas species based upon 16s-23s rdna intergenic spacer sequences.phylogenetic relationships of 17 species of xanthomonas were assessed based on analysis of 16s-23s rdna intergenic spacer (its) sequences; a higher level of resolution was obtained than that revealed by 16s rdna analysis. its sequences varied in size from 492 to 578 nt within the genus and the similarity among sequences ranged from 63 to 99%. major differences were found for the hyacinthi group, which included xanthomonas albilineans, xanthomonas hyacinthi, xanthomonas sacchari, xanthomonas thei ...200211931143
genome-wide sequencing data reveals virulence factors implicated in banana xanthomonas wilt.banana xanthomonas wilt is a newly emerging disease that is currently threatening the livelihoods of millions of farmers in east africa. the causative agent is xanthomonas campestris pathovar musacearum (xcm), but previous work suggests that this pathogen is much more closely related to species xanthomonas vasicola than to x. campestris. we have generated draft genome sequences for a banana-pathogenic strain of xcm isolated in uganda and for a very closely related strain of x. vasicola pathovar ...201020695894
genomes-based phylogeny of the genus xanthomonas.the genus xanthomonas comprises several plant pathogenic bacteria affecting a wide range of hosts. despite the economic, industrial and biological importance of xanthomonas, the classification and phylogenetic relationships within the genus are still under active debate. some of the relationships between pathovars and species have not been thoroughly clarified, with old pathovars becoming new species. a change in the genus name has been recently suggested for xanthomonas albilineans, an early br ...201222443110
genome sequencing of xanthomonas vasicola pathovar vasculorum reveals variation in plasmids and genes encoding lipopolysaccharide synthesis, type-iv pilus and type-iii secretion effectors.xanthomonas vasicola pathovar vasculorum (xvv) is the bacterial agent causing gumming disease in sugarcane. here, we compare complete genome sequences for five isolates of xvv originating from sugarcane and one from maize. this identified two distinct types of lipopolysaccharide synthesis gene clusters among xvv isolates: one is similar to that of xanthomonas axonopodis pathovar citri (xac) and is probably the ancestral type, while the other is similar to those of the sugarcane-inhabiting specie ...201425437615
biochemical and molecular tools reveal two diverse xanthomonas groups in bananas.xanthomonas campestris pv. musacearum (xcm) causing the banana xanthomonas wilt (bxw) disease has been the main xanthomonad associated with bananas in east and central africa based on phenotypic and biochemical characteristics. however, biochemical methods cannot effectively distinguish between pathogenic and non-pathogenic xanthomonads. in this study, gram-negative and yellow-pigmented mucoid bacteria were isolated from bxw symptomatic and symptomless bananas collected from different parts of u ...201626805624
rapid, specific, simple, in-field detection of xanthomonas campestris pathovar musacearum by loop-mediated isothermal amplification.to develop and evaluate a loop-mediated isothermal amplification (lamp) assay for xanthomonas campestris pathovar musacearum (xcm), the causal agent of banana xanthomonas wilt, a major disease of banana in africa.201526425811
detection and characterization of xanthomonas vasicola pv. vasculorum (cobb 1894) comb. nov. causing bacterial leaf streak of corn in the united states.bacterial leaf streak of corn (zea mays) recently reached epidemic levels in three corn-growing states, and has been detected in another six states in the central united states. xanthomonas vasicola was identified as the causal agent of this disease. a multi-locus sequence alignment (mlsa) of six housekeeping genes and comparison of average nucleotide identity (ani) from draft genome sequence were used to confirm phylogenetic relationships and classification of this bacterium relative to other x ...201728677478
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