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persistent infection of pets within a household with three bartonella species.we monitored by blood culture and immunofluorescence assay (ifa) bartonella infection in one dog and eight cats in a household to determine the prevalence and persistence of the infection as well as its transmissibility to humans. ectoparasite control was rigorously exercised. during a 3-year period, bartonella clarridgeiae was recovered from one cat on two occasions, and b. henselae was isolated from another cat on four occasions. during a 16-month period, b. vinsonii subsp. berkhoffii was isol ...19989621208
chest-wall abscess due to cat-scratch disease (csd) in an adult with antibodies to bartonella clarridgeiae: case report and review of the thoracopulmonary manifestations of csd.we describe a patient who presented with a massive chest-wall abscess after a severe debilitating illness that lasted 3 months. steroid therapy, administered for 4 weeks, masked the slow development of an extensive axillary and chest-wall abscess. after multiple negative tests, the patient's prolonged illness was diagnosed as cat-scratch disease (csd). an indirect fluorescent antibody test revealed that two convalescent serum samples were positive for igg to bartonella clarridgeiae, but no other ...19989709886
prevalence of bartonella henselae and bartonella clarridgeiae in an urban indonesian cat population.we studied evidence of bartonella henselae and bartonella clarridgeiae infection in 54 cats living in jakarta, indonesia. by using an indirect immunofluorescence assay, we found immunoglobulin g antibody to b. henselae in 40 of 74 cats (54%). the blood of 14 feral cats was cultured on rabbit blood agar plates for 28 days. bartonella-like colonies were identified as b. henselae or b. clarridgeiae by using restriction fragment length polymorphism analysis and direct sequencing of the pcr amplicons ...19999874661
clinical and pathologic evaluation of chronic bartonella henselae or bartonella clarridgeiae infection in cats.human bartonella infections result in diverse medical presentations, whereas many cats appear to tolerate chronic bacteremia without obvious clinical abnormalities. eighteen specific-pathogen-free cats were inoculated with bartonella henselae- and/or bartonella clarridgeiae-infected cat blood and monitored for 454 days. relapsing bacteremia did not correlate with changes in protein profiles or differences in antigenic protein recognition. intradermal skin testing did not induce a delayed type hy ...199910203518
bartonella henselae and bartonella clarridgeiae infection in domestic cats from the philippines.one hundred seven domestic cats from the philippines were serologically tested to establish the prevalence of bartonella infection. a subset of 31 of these cats also had whole blood collected to tentatively isolate bartonella strains. bartonella henselae and b. clarridgeiae were isolated from 19 (61%) of these cats. bartonella henselae type i was isolated from 17 (89%) of the 19 culture-positive cats. six cats (31%) were infected with b. clarridgeiae, of which four were coinfected with b. hensel ...199910348234
prevalence of bartonella henselae, bartonella clarridgeiae and the 16s rrna gene types of bartonella henselae among pet cats in japan.the authors investigated bacteriologically the prevalence of bartonella infection among 690 pet cats derived from 10 private animal hospitals in six cities (sapporo, hokkaido prefecture; sendai, miyagi prefecture; joetsu, niigata prefecture; fujisawa, kanagawa prefecutre; kyoto, kyoto prefecture; sanda, hyogo prefecutre) and 4 counties (mishima, osaka prefecture; hikawa, shimane prefecture; aira, kagoshima prefecture; shimajiri, okinawa prefecture) located from the north to the south of japan. b ...200010770599
characterization of bartonella clarridgeiae flagellin (flaa) and detection of antiflagellin antibodies in patients with lymphadenopathy.cat scratch disease (csd) is a frequent clinical outcome of bartonella henselae infection in humans. recently, two case reports indicated bartonella clarridgeiae as an additional causative agent of csd. both pathogens have been isolated from domestic cats, which are considered to be their natural reservoir. b. clarridgeiae and b. henselae can be distinguished phenotypically by the presence or absence of flagella, respectively. separation of the protein content of purified flagella of b. clarridg ...200010921956
bartonella schoenbuchii sp. nov., isolated from the blood of wild roe deer.the genus bartonella comprises two human-specific pathogens and a growing number of zoonotic or animal-specific species. domesticated as well as wild mammals can serve as reservoir hosts for the zoonotic agents and transmission to humans may occur by blood sucking arthropods or by direct blood to blood contact. humans may come into intimate contact with free-ranging mammals during hunting, especially during evisceration with bare hands, when accidental blood to blood contact frequently occurs. t ...200111491358
aortic valve endocarditis in a dog due to bartonella clarridgeiae.we report the first documented case of endocarditis associated with bartonella clarridgeiae in any species. b. clarridgeiae was identified as a possible etiological agent of human cat scratch disease. infective vegetative valvular aortic endocarditis was diagnosed in a 2.5-year-old male neutered boxer. historically, the dog had been diagnosed with a systolic murmur at 16 months of age and underwent balloon valvuloplasty for severe valvular aortic stenosis. six months later, the dog was brought t ...200111574571
characterization of the first bartonella henselae strain isolated from a cat in italy.bartonella henselae has been identified and characterized for the first time in italy. a strain, designed pavia-1, was isolated from the blood of a cat whose owner developed cat scratch disease (csd). pavia-1 and two american b. henselae strains (houston-1, atcc 49882, type i and strain 269608, uc davis, type ii) were compared by whole-cell fatty analysis (cfa), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) for protein profiles, western immunoblotting (wb) for reactivity w ...200212135236
detection of bartonella henselae and bartonella clarridgeiae dna in hepatic specimens from two dogs with hepatic disease.a 4-year-old basset hound and a 6-year-old doberman pinscher were referred for diagnostic evaluation following documentation of persistently increased hepatic enzyme activities and hepatic dysfunction. histologic evaluation of hepatic biopsy specimens from the 2 dogs revealed granulomatous hepatitis in the basset hound and lymphocytic hepatitis with fibrosis and copper accumulation in the doberman pinscher. no etiologic agents were identified histologically. bartonella henselae dna was subsequen ...200312523479
identification of rickettsia spp. and bartonella spp. in ffrom the thai-myanmar border.during a survey for possible rickettsial vectors in villages of the central part of the thai-myanmar border from september 2001 to february 2002, four species of fleas were collected from common peridomestic animals. all fleas were tested by pcr to detect dna of bacteria of the genera rickettsia (glta and ompb genes) and bartonella (its and ftsz genes). sequencing of pcr-amplified products was done using glta fragments for rickettsia and ftsz fragments for bartonella: two genotypes related to ri ...200312860622
prevalence of bartonella clarridgeiae and bartonella henselae in domestic cats from france and detection of the organisms in erythrocytes by immunofluorescence.the prevalence of bartonella infection in a pet cat population from france was found to be 8.1% (8 of 99 cats). the intraerythrocytic location of bartonella clarridgeiae is shown for the first time, and we show that immunofluorescence detection of the organism in erythrocytes correlates with the number of bacteria in blood.200415013998
bartonella clarridgeiae and b. henselae in dogs, gabon. 200415672535
prevalence of selected infectious disease agents in cats from arizona.the objective of this study was to use polymerase chain reaction (pcr) assays to determine the prevalence of ehrlichia species, anaplasma phagocytophilum, mycoplasma haemofelis, 'candidatus mycoplasma haemominutum' and bartonella species from feral and relinquished cats in phoenix and nogales, arizona. dna from one or more of the organisms was amplified from 31 of 112 blood samples (27.7%). dna consistent with bartonella clarridgeiae 15 (13.4%), bartonella henselae 14 (12.5%), 'candidatus m haem ...200616443383
prevalence of rickettsia felis-like and bartonella spp. in ctenocephalides felis and ctenocephalides canis from la rioja (northern spain).our aim was to determine the presence of rickettsia spp. and bartonella spp. in ctenocephalides felis and ctenocephalides canis from la rioja (spain). a total of 88 specimens were tested by polymerase chain reaction (pcr) using glta and ompb genes as targets for rickettsia spp., and 16s rrna and ribc genes for bartonella spp. rickettsia felis-like (28.4%), bartonella clarridgeiae (6.8%), and bartonella henselae (3.4%) were detected in ctenocephalides spp. other bartonella sp. different from b. c ...200617114721
bartonella infections in fleas (siphonaptera: pulicidae) and lack of bartonellae in ticks (acari: ixodidae) from hungary.fleas (95 pulex irritans, 50 ctenocephalides felis, 45 ctenocephalides canis) and ixodid ticks (223 ixodes ricinus, 231 dermacentor reticulatus, 204 haemaphysalis concinna) were collected in hungary and tested, in assays based on pcr, for bartonella infection. low percentages of p. irritans (4.2%) and c. felis (4.0%) were found to be infected. the groel sequences of the four isolates from p. irritans were different from all the homologous sequences for bartonellae previously stored in genbank bu ...200617252927
gray foxes (urocyon cinereoargenteus) as a potential reservoir of a bartonella clarridgeiae-like bacterium and domestic dogs as part of a sentinel system for surveillance of zoonotic arthropod-borne pathogens in northern california.two species of bartonella, a novel bartonella clarridgeiae-like bacterium and b. vinsonii subsp. berkhoffii, were isolated from rural dogs and gray foxes in northern california. a novel b. clarridgeiae-like species was isolated from 3 (1.7%) of 182 dogs and 22 (42%) of 53 gray foxes, while b. vinsonii subsp. berkhoffii was isolated from 1 dog (0.5%) and 5 gray foxes (9.4%). pcr and dna sequence analyses of the citrate synthase (glta) gene and the 16s-23s intergenic spacer region suggested that s ...200717553970
mixed infections, cryptic diversity, and vector-borne pathogens: evidence from polygenis fleas and bartonella species.coinfections within hosts present opportunities for horizontal gene transfer between strains and competitive interactions between genotypes and thus can be a critical element of the lifestyles of pathogens. bartonella spp. are alphaproteobacteria that parasitize mammalian erythrocytes and endothelial cells. their vectors are thought to be various biting arthropods, such as fleas, ticks, mites, and lice, and they are commonly cited as agents of various emerging diseases. coinfections by different ...200717693558
phylogenetic analysis of bartonella detected in rodent fleas in yunnan, china.previous studies have demonstrated a diversity of bartonella spp. in rodent populations in yunnan province, china. although bartonella spp. have been isolated from cat fleas and cattle ticks collected from their animal hosts, little is known about bartonella carried by rodent fleas. in this study, bartonella dna was detected by polymerase chain reaction (pcr) in two of five species of rodent fleas. these included xenopsylla cheopis and ctenophthalmus lushuiensis, which were collected from rattus ...200717984255
vector-borne infections in cats: molecular study in barcelona area (spain).previous serological surveys have reported the presence of different organisms in cats from spain but little reports exist about the exact identity of these organisms. the purpose of the study reported here was to assess the presence of dna of several vector-borne infections in a population of cats from barcelona area. one hundred blood samples obtained from cats admitted to the uab-vth were entered into the study and classified as healthy (n=48) or unhealthy (n=52). edta-blood samples were assa ...200818079064
ectoparasites and associated pathogens of free-roaming and captive animals in zoos of south carolina.a survey of ectoparasites and their associated pathogens was conducted in two south carolina zoos, from 2004 to 2007. dead, wild birds and mammals, as well as captive animals examined during routine veterinary checks constituted the study populations. ectoparasites were tested for species of anaplasma, bartonella, coxiella burnetii, ehrlichia, rickettsia, and trypanosoma. forty-six species of ectoparasites were collected from 133 free-roaming and captive hosts and their associated nesting and be ...200918973443
seroprevalence of bartonella spp. in the endangered island fox (urocyon littoralis).bartonella clarridgeiae-like strains, presently b. rochalimae, were isolated in gray foxes (urocyon cinereoargenteus) in mainland california. the objective of this study was to investigate the presence of bartonella infection in the endangered island fox (urocyon littoralis) found only on the channel islands off the californian coast. between 2001 and 2004, 263 serum samples were collected. antibodies against bartonella vinsonii subsp. berkhoffii (bvb) and b. clarridgeiae (bc) were detected usin ...200919058928
bartonella clarridgeiae in a cat in the uk. 200919136687
investigation of bartonella henselae in cats in ankara, turkey.the purpose of this study was to determine bartonella henselae prevalance in cats in ankara. whole bloods and sera collected from 256 cats were investigated for the presence feline bartonella species by culture and sera were tested for the presence of antibodies against b. henselae igg using immunofluorescence assay. bartonella species were isolated by blood culture from 24 (9.4%) cats. bartonella isolates were subjected to restriction fragment length polymorphism (rflp) by using taqi and hhai e ...200918990198
microbial culture of blood samples and serologic testing for bartonellosis in cats with chronic rhinosinusitis.to assess the role of bartonella spp in chronic rhinosinusitis (crs) by determining detection rates for the organism by serologic testing and microbial culture of blood samples for bartonella spp in cats with crs and control cats (cats with other nasal diseases, cats with systemic illnesses, and healthy cats).200818828717
molecular evidence for bartonella spp. in cat and dog fleas from germany and france.nine hundred and fifty-two fleas were collected from 148 cats and 133 dogs at 18 widely distributed geographic locations in germany and france and examined for the presence of six different bartonella spp. (bartonella bacilliformis, bartonella clarridgeiae, bartonella elizabethae, bartonella henselae, bartonella quintana, bartonella vinsonii subsp. berkhoffii) by pcr. thirty-five specimens (3.7%) tested positive for either b. henselae (14 positive fleas) or b. clarridgeiae (21 positive fleas). d ...200818489542
rickettsia felis and bartonella clarridgeiae in fleas from new caledonia.dog fleas collected in new caledonia harbored flea-borne pathogens rickettsia felis and bartonella clarridgeiae in 81% and 5%, respectively.201120569017
evaluation of indirect fluorescence antibody assay for detection of bartonella clarridgeiae and seroprevalence of b. clarridgeiae among patients with suspected cat scratch disease.the possibility of bartonella clarridgeiae being a causative agent of cat scratch disease (csd) was investigated by using indirect fluorescence antibody assays with 288 suspected csd patients. immunoglobulin g antibody to noncocultivated b. clarridgeiae was suitable only for detection of b. clarridgeiae antibody. significant cross-reactivity between bartonella henselae and b. clarridgeiae was noted, and no csd case caused by b. clarridgeiae was detected.200415243113
molecular evidence of bartonella infection in domestic dogs from algeria, north africa, by polymerase chain reaction (pcr).bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. bartonella dna extracted from blood samples was obtained from domestic dogs in algiers, algeria. polymerase chain reaction (pcr) and dna sequence analyses of the ftsz gene and the 16s-23s intergenic spacer region (its) were performed. three bartonella species: bartonella vinsonii subsp. berkhoffii, bartonella clarridgeiae, and bartonells elizabethae were ...201020682871
[cat-scratch disease neuroretinitis].we report a case of cat-scratch disease neuroretinitis for which systemic and ocular investigations proved the responsibility of bartonella henselae. an 11-year-old boy was referred to the hospital in november 2002 for severe visual loss in the left eye over the preceding 2 weeks. at the same time, he also developed a flu-like illness. the best corrected acuity in the left eye was counting fingers at 30 cm. posterior segment examination on the left eye showed an optic disk edema with papillary a ...200415039632
detection and characterization of feline bartonella henselae in the czech republic.the aims of the study were to characterize isolates of bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of prague, czech republic. five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubated without previous freezing. four of these isolates originated from flea infested stray cats (n=6) and one from a shelter cat without any ectoparasites (n=21). none of the 34 previously frozen specime ...200312695049
a prospective study of cat-scratch disease in lima-peru.cat-scratch disease (csd) is a benign lymphadenitis that may progress to severe or recurrent forms, and it is occasionally associated with morbidity. between january of 1998 and march of 1999, forty-three suspected csd patients were assessed in the hospital cayetano heredia and the instituto de salud del niño, in lima, peru. twelve patients had a confirmed diagnosis, 8 of whom were women, and the mean age was 10 years old. the majority (53%) of the cases were encountered in the summer. all patie ...200212532216
prevalence of rickettsia felis and the first identification of bartonella henselae fizz/cal-1 in cat fleas (siphonaptera: pulicidae) from taiwan.cat fleas (ctenocephalides felis [bouché]) are the primary ectoparasites of dog and cat populations. in this study, we report the monthly population dynamics of rickettsia felis and bartonella spp. (two zoonotic pathogens that can cause human disease) in cat fleas collected from dogs and cats in taipei, taiwan, from december 2006 to december 2007. natural r. felis infection in individual cat fleas was assessed by polymerase chain reaction (pcr) using prf-, ompb-, and glta-specific primer pairs. ...201121485388
absence of zoonotic bartonella species in questing ticks: first detection of bartonella clarridgeiae and rickettsia felis in cat fleas in the netherlands.abstract:201121501464
infection and re-infection of domestic cats with various bartonella species or types: b. henselae type i is protective against heterologous challenge with b. henselae type ii.four bartonella species have been isolated from domestic cats, of which two serotypes/genotypes of bartonella henselae and possibly b. clarridgeiae are human pathogens, causing cat scratch disease (csd).our objectives were to evaluate infection and potential cross-protection during re-infection in domestic cats with various bartonella species or types.thirty-six cats were primarily inoculated with b. henselae type i (n=16), b. henselae type ii (n=10), b. clarridgeiae (n=6) or b. koehlerae (n=4). ...200312488072
genomic variation of bartonella henselae strains detected in lymph nodes of patients with cat scratch disease.bartonella henselae is the primary agent of cat scratch disease (csd). in order to study the genetic variation of b. henselae and the correlation of the various genotypes with epidemiological and clinical findings, two seminested, groel- and pap31-based pcr assays were carried out with specimens from 273 patients. amplicons were sequenced to determine the genotype of the causative bartonella species. compared to our reference intergenic spacer region-based pcr, the groel- and pap31-based assays ...200211880432
bartonella and rickettsia in arthropods from the lao pdr and from borneo, malaysia.rickettsioses and bartonelloses are arthropod-borne diseases of mammals with widespread geographical distributions. yet their occurrence in specific regions, their association with different vectors and hosts and the infection rate of arthropod-vectors with these agents remain poorly studied in south-east asia. we conducted entomological field surveys in the lao pdr (laos) and borneo, malaysia by surveying fleas, ticks, and lice from domestic dogs and collected additional samples from domestic c ...201122153360
phylogenetic classification of bartonella species by comparing groel sequences.bartonella is a bacterial genus classified in the alpha-proteobacteria on the basis of 165 rdna sequence comparison. the highly conserved heat-shock chaperonin protein, groel, has proved to be a valuable resolving tool to classify ten bartonella species. the groel gene was amplified and sequenced from ten bartonella isolates: bartonella alsatica, bartonella vinsonii subsp. arupensis, bartonella taylorii, bartonella tribocorum, bartonella birtlesii, bartonella henselae marseille (urlly8), b. hens ...200211837299
bartonella clarridgeiae in fleas, tahiti, french polynesia. 201121888825
bartonella clarridgeiae, b. henselae and rickettsia felis in fleas from morocco.a total of 554 fleas were collected in the moroccan casablanca and tiznit regions from domesticated animals and ruminants between august 2007 and october 2008 and were tested for the presence of rickettsia spp. and bartonella spp. using molecular methods. for the first time in morocco, we found rickettsia felis, the agent of flea-borne spotted fever in ctenocephalides felis; b. henselae, an agent of cat scratch disease; and bartonella clarridgeiae, a cat pathogen and potentially a human pathoge ...201122185943
bacterial and protozoal agents of feline vector-borne diseases in domestic and stray cats from southern portugal.feline vector-borne diseases (fvbd) have emerged in recent years, showing a wider geographic distribution and increased global prevalence. in addition to their veterinary importance, domestic cats play a central role in the transmission cycles of some fvbd agents by acting as reservoirs and sentinels, a circumstance that requires a one health approach. the aim of the present work was to molecularly detect feline vector-borne bacteria and protozoa with veterinary and zoonotic importance, and to a ...201424655431
molecular phylogeny of the genus bartonella: what is the current knowledge?species of the genus bartonella are involved in an increasing variety of human diseases. in addition to the 14 currently recognized species, several bartonella strains have been recovered from a wide range of wild and domestic mammals in europe and america. such a high diversity of geographic distributions, animal reservoirs, arthropod vectors and pathogenic properties makes clarification of our knowledge about the phylogeny of bartonella species necessary. phylogenetic data have been inferred m ...200111410341
epidemiology of bartonella infection in domestic cats in france.blood samples were collected between february and june 1996 from a convenience sample of 436 domestic french cats living in paris and its environs and were tested for bartonella bacteremia and seropositivity. seventy-two cats (16.5%) were bartonella bacteremic, of which 36 cats (50%) were infected with bartonella henselae type ii (b.h. ii) only, 15 cats (21%) were infected with bartonella clarridgeiae (b.c.) only, and 11 cats (15%) were infected with b. henselae type i (b.h. i) only. eight cats ...200111295338
genetic variability and prevalence of bartonella henselae in cats in berlin, germany, and analysis of its genetic relatedness to a strain from berlin that is pathogenic for humans.nineteen bartonella henselae strains and one bartonella clarridgeiae strain were isolated from blood samples of 97 pet cats and 96 stray cats from berlin, germany, indicating prevalence rates of 1 and 18.7%, respectively, for b. henselae and 0 and 1%, respectively, for b. clarridgeiae. eighteen of 19 b. henselae isolates corresponded to 16s rrna type ii. pulsed-field gel electrophoresis (pfge) analysis revealed seven different pfge types among the feline b. henselae strains. interestingly, all f ...200111158141
study on ticks and tick-borne zoonoses in public parks in italy.a survey on tick density and on tick-borne zoonoses was carried out in four public parks in the outskirts of imola (northern italy) from june to october 2006. all stages of ixodes ricinus and only larvae of riphicephalus sanguineus were recovered by dragging, performed on 100-m transects. almost all ticks (99%) were harvested in one park. i. ricinus density (nymphs/100 m(2) ) ranged from 0 in park l to 6.3 in park f. nymphs and adults of i. ricinus were subjected to pcr for anaplasma phagocytoph ...201222551055
recent advances in diagnosis and treatment of cat scratch disease.the cause of cat scratch disease (csd), first described in france in 1950 and in the united states in 1951, was unknown until 1983 when the bacterium in lymph nodes was detected using a warthin-starry silver stain. afipia felis has been an infrequent cause of csd since1988, when this gram-negative bacterium was first isolated from 10 patients with csd. in 1992 bartonella organisms were isolated from immunocompetent and immunocompromised patients. an indirect fluorescent antibody test to detect b ...200011095849
a bacteriophage-like particle from bartonella bacilliformis.bartonella bacilliformis and bartonella henselae, the respective agents of oroya fever and cat-scratch disease in humans, are known to produce bacteriophage-like particles (blps) that package 14 kbp segments of the host chromosome. data from this study suggest that other bartonella species including bartonella quintana, bartonella doshiae and bartonella grahamii also contain similar blps, as evidenced by the presence of a 14 kbp extrachromosomal dna element in their genomes, whereas bartonella e ...200010746763
efficacy of enrofloxacin or doxycycline for treatment of bartonella henselae or bartonella clarridgeiae infection in cats.enrofloxacin and doxycycline are antimicrobial agents used to treat bacterial diseases of cats. in vitro susceptibility data indicate that either drug should be effective against bartonella species. in vivo efficacies of these drugs for eradication of chronic bartonella henselae or bartonella clarridgeiae infections were examined in 18 experimentally infected cats and 25 naturally exposed cats treated with enrofloxacin (22.7 mg given orally [po] every 12 h [q12h] [14 days, n = 10; 28 days, n = 1 ...19979371348
intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in continental and insular greece.this survey investigated the distribution of various intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in four regions of greece. a total number of one hundred and fifty cats living in three islands (crete, mykonos and skopelos) and in athens municipality was established as a realistic aim to be accomplished in the study areas. all cats were examined with different microscopic, serological and molecular assays aiming at evaluating the occurrence of intestinal ...201728141857
prevalence of bartonella species infections in cats in southern germany.bartonella species are zoonotic pathogens, and infections in cats are common. however, prevalence in cats in southern germany is still unknown. therefore, prevalence of bartonella species dna in blood of 479 southern german cats was determined using a previously published conventional pcr targeting a fragment of the 16s-23s rrna intergenic spacer region. associations between bartonella bacteraemia, housing conditions, feline immunodeficiency virus (fiv) and feline leukaemia virus (felv) status, ...201728130406
coinfection with bartonella clarridgeiae and bartonella henselae and with different bartonella henselae strains in domestic cats.bartonella clarridgeiae and several strains of bartonella henselae, the agent of cat scratch disease, with variations in the 16s rrna gene have been found to infect the blood of cats. an epidemiologic study of bartonella infection in domestic french cats revealed that of 436 cats sampled, 5 cats (1.1%) were coinfected with b. henselae and b. clarridgeiae and 2 cats (0.5%) were coinfected with two strains of b. henselae with variations in the 16s rrna gene, b. henselae type i and type ii. in an i ...19979230394
bartonella clarridgeiae, a newly recognized zoonotic pathogen causing inoculation papules, fever, and lymphadenopathy (cat scratch disease).shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. initial blood cultures from the cat and veterinarian were sterile. repeat cultures from the cat grew bartonella-like organisms with lophotrichous flagella. sera from the veterinarian were not reactive against bartonella henselae, b. quintana, or b. elizabethae antigens but were seroreactive (reciprocal titer, 1,024) against ...19979196200
molecular evidence of vector-borne pathogens in dogs and cats and their ectoparasites in algiers, algeria.in algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. the aim of the present work was to detect by qpcr vector-associated bacteria in stray dogs and cats and their ectoparasites from algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. coxiella burnetii and bartonella henselae were identified in 1/117 (0.85%) dog individually. ehrlichia canis dna was detected in 17/117 ...201627012917
fleas and flea-associated bartonella species in dogs and cats from peru.in the present study, we investigated 238 fleas collected from cats and dogs in three regions of peru (ancash, cajamarca, and lima) for the presence of bartonella dna. bartonella spp. were detected by amplification of the citrate synthase gene (16.4%) and the 16s-23s intergenic spacer region (20.6%). bartonella rochalimae was the most common species detected followed by bartonella clarridgeiae and bartonella henselae. our results demonstrate that dogs and cats in peru are infested with fleas har ...201526363063
assessment of a quantitative 5' nuclease real-time polymerase chain reaction using the nicotinamide adenine dinucleotide dehydrogenase gamma subunit (nuog) for bartonella species in domiciled and stray cats in brazil.the objective of this study was to develop a quantitative 5' nuclease real-time polymerase chain reaction (pcr) assay to diagnose infections caused by bartonella species.201626138812
molecular detection of bartonella clarridgeiae in domestic cats from midwest brazil. 201526100436
prevalence of bartonella henselae and bartonella clarridgeiae in stray cats.the aim of the present work was to determine by blood culture the prevalence of blood infection with bartonella species in a well-defined, european, urban stray cat population. therefore, 94 stray cats were trapped from 10 cat colonies. blood samples of these cats were cultured on both blood agar and liquid medium in order to raise the likelihood of bacterial detection. fifty blood samples (53%) gave a positive culture result for bartonella species. isolate identification was performed by sequen ...19979163438
detection and identification of bartonella sp. in fleas from carnivorous mammals in andalusia, spain.a total of 559 fleas representing four species (pulex irritans, ctenocephalides felis, ctenocephalides canis and spilopsyllus cuniculi) collected on carnivores (five iberian lynx lynx pardinus, six european wildcat felis silvestris, 10 common genet genetta genetta, three eurasian badger meles meles, 22 red fox vulpes vulpes, 87 dogs and 23 cats) in andalusia, southern spain, were distributed in 156 pools of monospecific flea from each carnivore, and tested for bartonella infection in an assay ba ...200919941605
[biological and molecular characteristics of a cat-borne bartonella clarridgeiae].to characterize a bartonella strain m9hn-shq from a blood culture of cat from henan province,china.200919621628
first detection of rickettsia felis and bartonella clarridgeiae in fleas from laos. 200919438611
prevalence of bartonella henselae and bartonella clarridgeiae in cats and dogs in korea.blood, saliva, and nail samples were collected from 54 dogs and 151 cats and analyzed for the presence of bartonella henselae with a novel nested polymerase chain reaction (pcr) method. bartonella (b.) henselae was detected in feral cat blood (41.8%), saliva (44.1%), and nail (42.7%) samples. b. henselae was also detected in pet cat blood (33.3%), saliva (43.5%), and nail (29.5%) samples and in pet dog blood (16.6%), saliva (18.5%), and nail (29.6%) samples. nine samples were infected with b. cl ...200919255530
the prevalence of bartonella, hemoplasma, and rickettsia felis infections in domestic cats and in cat fleas in ontario.the prevalence of persistent bacteremic bartonella spp. and hemoplasma infections was determined in healthy pet cats in ontario. blood samples from healthy cats sent to a diagnostic laboratory for routine health assessment over the course of 1 y were tested for bartonella spp. using both polymerase chain reaction (pcr) and blood culture, and for the presence of hemoplasma by pcr. the overall prevalence of bartonella spp. by pcr and by culture combined was 4.3% (28/646) [3.7% (24/646) bartonella ...200819086373
molecular detection of bartonella henselae and bartonella clarridgeiae in clinical samples of pet cats from southern italy.bartonella henselae is considered an emerging pathogen of veterinary and medical interest that can be occasionally transmitted to humans. cats are considered to be the only reservoir host for b. henselae. in this study, we used a nested-pcr assay to investigate the prevalence of b.henselae and bartonella clarridgeiae dna in peripheral blood samples, fine needle lymph node aspirate specimens and oral swabs from 85 cats in order to develop an easy diagnostic strategy for the selection of infection ...201019963231
bartonellosis, an increasingly recognized zoonosis.cat scratch disease is the most common zoonotic infection caused by bartonella bacteria. among the many mammals infected with bartonella spp., cats represent a large reservoir for human infection, as they are the main reservoir for bartonella henselae, bartonella clarridgeiae and bartonella koehlerae. bartonella spp. are vector-borne bacteria, and transmission of b. henselae by cat fleas occurs mainly through infected flea faeces, although new potential vectors (ticks and biting flies) have been ...201020148999
isolation and characterization of bartonella strains in cats in italy.bartonella henselae and bartonella clarridgeiae were isolated in two cats in sardinia, italy. infection by b. clarridgeiae was characterized by fever and submandibular lymph nodes enlargement while b. henselae infection was asymptomatic. this is the first report of b. clarridgeiae in a cat in italy and the first isolation of b. henselae and b. clarridgeiae in sardinia.201020345572
seroepidemiology of bartonella infection in gray foxes from texas.gray foxes (urocyon cinereoargenteus) were shown to be naturally infected with bartonella rochalimae, a bartonella species similar to bartonella clarridgeiae (b.c.), and bartonella vinsonii subspecies berkhoffii (b.v.berkhoffii) in northern california. a serological survey was performed to investigate the presence of bartonella infection in 132 gray foxes from west/central texas. using an immunofluorescence antibody test directed against b.v.berkhoffii and b.c., the antibody prevalence was 50% ( ...201222217178
combined mlst and aflp typing of bartonella henselae isolated from cats reveals new sequence types and suggests clonal evolution.bartonella species are gram-negative, fastidious bacteria. bartonella henselae is found in cats and transmitted to humans via cat scratches or bites causing cat-scratch disease, characterized by clinical symptoms with varying severity. the prevalence of bartonellosis among humans in germany appears to be high, and severe clinical cases have been described. however, epidemiological data of b. henselae in cats are rare. in this study we determined the detection rates of bartonella ssp. in cats by ...201020863631
prevalence of bartonella henselae and bartonella clarridgeiae in cats in the south of brazil: a molecular study.bartonella spp are the causative agent of cat scratch disease in humans. cats are the natural reservoir of these bacteria and may infect humans through scratches, bites or fleas. blood samples from 47 cats aged up to 12 months were collected for this study. all animals were lodged in municipal animal shelters in the vale do sinos region, rio grande do sul, brazil. bartonella spp were detected by genus-specific polymerase chain reaction (pcr) and when the pcr was positive, the species were determ ...201021120356
bartonella infection in shelter cats and dogs and their ectoparasites.abstract mainly through vector transmission, domestic cats and dogs are infected by several bartonella spp. and represent a large reservoir for human infections. this study investigated the relationship of prevalences of bartonella infection in shelter dogs and cats and various ectoparasite species infesting them (fleas, ticks, and lice). moreover, relationships between bartonella infection and animal gender and age and presence of ectoparasites were analyzed. blood samples were collected from 1 ...201021142966
rickettsia, ehrlichia, anaplasma, and bartonella in ticks and fleas from dogs and cats in bangkok.abstract flea and tick specimens (5-10 fleas or ticks) on dogs and cats from various sites in bangkok were tested by polymerase chain reaction and dna sequencing to detect dna of bacteria rickettsia (glta and 17 kda genes), anaplasmataceae (16s rrna gene), and bartonella (pap31 and its genes). we confirmed that rickettsia sp. related to rickettsia felis was detected in 66 of 98 (67.4%) flea specimens from dogs, whereas 8 bartonella henselae and 2 bartonella clarridgeiae were detected in 10 of 54 ...201121612535
association between bartonella species infection and disease in pet cats as determined using serology and culture.this study's objective was to determine whether a relationship exists between infection or seropositivity to bartonella species and clinical illness in cats. blood samples were obtained for bartonella species isolation and immunofluorescent antibody serology from 298 cats presenting to a tertiary referral hospital. medical records were searched and the history, physical examination findings and the results of diagnostic testing relating to the visit at which bartonella species testing was perfor ...201020570199
zoonotic bartonella species in fleas and blood from red foxes in australia.abstract bartonella are arthropod-borne, fastidious, gram-negative, and aerobic bacilli distributed by fleas, lice, sand flies, and, possibly, ticks. the zoonotic bartonella species, bartonella henselae and bartonella clarridgeiae, which are the causes of cat scratch disease and endocarditis in humans, have been reported from cats, cat fleas, and humans in australia. however, to date, there has been no report of b. henselae or b. clarridgeiae in australian wild animals and their ectoparasites. ...201121919728
multiplex sybr® green-real time pcr (qpcr) assay for the detection and differentiation of bartonella henselae and bartonella clarridgeiae in cats.a novel sybr® green-real time polymerase chain reaction (qpcr) was developed to detect two bartonella species, b. henselae and b. clarridgeiae, directly from blood samples. the test was used in blood samples obtained from cats living in animal shelters in southern brazil. results were compared with those obtained by conventional pcr targeting bartonella spp. among the 47 samples analyzed, eight were positive using the conventional pcr and 12 were positive using qpcr. importantly, the new qpcr de ...024626408
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
molecular epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in japanese iriomote and tsushima leopard cats.the iriomote cat (ic; prionailurus iriomotensis) and the tsushima leopard cat (tlc; prionailurus bengalensis euptilura) are endangered wild felids in japan. as a part of ongoing conservation activities, we conducted a molecular, epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in wild ic and tlc populations. blood samples (47 from 33 individual ic; 22 from 13 tlc) were collected between august 2002 and january 2011. using pcr analysis, we confirmed the presence of bartonel ...201323778615
prevalence and potential risk factors for bartonella infection in tunisian stray dogs.bartonellae are blood-borne and vector-transmitted pathogens, some are zoonotic, which have been reported in several mediterranean countries. transmission from dogs to humans is suspected, but has not been clearly demonstrated. our objectives were to determine the seroprevalence of bartonella henselae, bartonella vinsonii subsp. berkhoffii, bartonella clarridgeiae, and bartonella bovis (as a proxy for candidatus bartonella merieuxii) in stray dogs from tunisia, identify the bartonella species in ...201728346866
experimental infection of cats with afipia felis and various bartonella species or subspecies.based upon prior studies, domestic cats have been shown to be the natural reservoir for bartonella henselae, bartonella clarridgeiae and bartonella koehlerae. however, other bartonella species, such as bartonella vinsonii subsp. berkhoffii, bartonella quintana or bartonella bovis (ex weissii) have been either isolated from or bartonella dna sequences pcr amplified and sequenced. in the late 1980s, before b. henselae was confirmed as the etiological agent of cat scratch disease, afipia felis had ...201424972870
bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the caatinga biome, northeastern brazil: a serological and molecular study.this study verified the occurrence of bartonella spp. in dogs, cats, wild mammals and their ectoparasites in petrolina and lagoa grande counties, pernambuco, located in a semi-arid region in northeastern brazil. anti-bartonella spp. antibodies were detected by indirect immunofluorescence assay (ifa) in 24.8% of dogs (27/109) and in 15% of cats (6/40). bartonella sp. dna was identified by pcr performed on dna extracted from blood and ectoparasites using primers targeting bartonella sp. glta and r ...201728249707
cat fleas (ctenocephalides felis) from cats and dogs in new zealand: molecular characterisation, presence of rickettsia felis and bartonella clarridgeiae and comparison with australia.the cat flea (ctenocephalides felis) is the most common flea species parasitising both domestic cats and dogs globally. fleas are known vectors of zoonotic pathogens such as vector borne rickettsia and bartonella. this study compared cat fleas from domestic cats and dogs in new zealand's north and south islands to australian cat fleas, using the mitochondrial dna (mtdna) marker, cytochrome c oxidase subunit i and ii (cox1, cox2). we assessed the prevalence of rickettsia and bartonella using genu ...201728115179
prevalence, hematological findings and genetic diversity of bartonella spp. in domestic cats from valdivia, southern chile.the present study determined the prevalence, hematological findings and genetic diversity of bartonella spp. in domestic cats from valdivia, southern chile. a complete blood count and nuog gene real-time quantitative pcr (qpcr) for bartonella spp. were performed in 370 blood samples from cats in valdivia, southern chile. nuog qpcr-positive samples were submitted to conventional pcr for the glta gene and sequencing for species differentiation and phylogenetic analysis. alignment of glta gene was ...201627938417
bartonella species in fleas from palestinian territories: prevalence and genetic diversity.bartonellosis is an infectious bacterial disease. the prevalence and genetic characteristics of bartonella spp. in fleas of wild and domestic animals from palestinian territories are described. flea samples (n=289) were collected from 121 cats, 135 dogs, 26 hyraxes and seven rats from northern (n=165), central (n=113), and southern palestinian territories (n=11). the prevalent flea species were: ctenocephalides felis (n=119/289; 41.2%), ctenocephalides canis (n=159/289; 55%), and xenopsylla sp. ...201425424254
bartonella clarridgeiae bacteremia detected in an asymptomatic blood donor.human exposure to bartonella clarridgeiae has been reported only on the basis of antibody detection. we report for the first time an asymptomatic human blood donor infected with b. clarridgeiae, as documented by enrichment blood culture, pcr, and dna sequencing.201525392353
bartonella clarridgeiae and bartonella vinsonii subsp. berkhoffii exposure in captive wild canids in brazil.summary wild canids are potential hosts for numerous species of bartonella, yet little research has been done to quantify their infection rates in south america. we sought to investigate bartonella seroprevalence in captive wild canids from 19 zoos in são paulo and mato grosso states, brazil. blood samples were collected from 97 wild canids belonging to four different native species and three european wolves (canis lupus). indirect immunofluorescent antibody testing was performed to detect the p ...201524892580
flea species infesting dogs in florida and bartonella spp. prevalence rates.several bartonella spp. associated with fleas can induce a variety of clinical syndromes in both dogs and humans. however, few studies have investigated the prevalence of bartonella in the blood of dogs and their fleas. the objectives of this study were to determine the genera of fleas infesting shelter dogs in florida, the prevalence of bartonella spp. within the fleas, and the prevalence of bartonella spp. within the blood of healthy dogs from which the fleas were collected. fleas, serum, and ...201424268654
bartonellae in domestic and stray cats from israel: comparison of bacterial cultures and high-resolution melt real-time pcr as diagnostic methods.to determine the occurrence of feline bartonellosis in israel, blood samples were collected from 179 stray and 155 domestic cats from 18 cities or villages in central and northcentral israel. samples were screened for bartonella infection by culture isolation and molecular detection using high-resolution melt (hrm) real-time pcr assay targeting the 16s-23s rrna internal transcribed spacer (its). all positive samples were confirmed by two additional hrm real-time pcr assays targeting two fragment ...201324107217
ctenocephalides felis an in vitro potential vector for five bartonella species.the blood-sucking arthropod ctenocephalides felis has been confirmed as a vector for bartonella henselae and is a suspected vector for bartonella clarridgeiae, bartonella quintana and bartonella koehlerae in bartonella transmission to mammals. to understand the absence of other bartonella species in the cat flea, we have developed an artificial flea-feeding method with blood infected successively with five different bartonella species. the results demonstrated the ability of these five bartonell ...201323200028
molecular characterisation of bartonella species in cats from são luís, state of maranhão, north-eastern brazil.bartonella species are fastidious bacteria that predominantly infect mammalian erythrocytes and endothelial cells and cause long-lasting bacteraemia in their reservoir hosts. reports that describe the epidemiology of bartonellosis in brazil are limited. this study aimed to detect and characterise bartonella spp dna from cat blood samples in são luís, maranhão, north-eastern brazil. among 200 cats tested for multiple genes, nine (4.5%) were positive for bartonella spp: six cats for bartonella hen ...201222990968
prevalence of bartonella species, hemoplasmas, and rickettsia felis dna in blood and fleas of cats in bangkok, thailand.flea infestations are common in thailand, but little is known about the flea-borne infections. fifty flea pools and 153 blood samples were collected from client-owned cats between june and august 2009 from veterinary hospitals in bangkok, thailand. total dna was extracted from all samples, and then assessed by conventional pcr assays. the prevalence rates of bartonella spp. in blood and flea samples were 17% and 32%, respectively, with dna of bartonella henselae and bartonella clarridgeiae being ...201222521739
human seroreactivity against bartonella species in the democratic republic of congo.to assess the presence and identity of bartonella species in a pool of human blood samples from drc congo.201121771478
relationship between the presence of bartonella species and bacterial loads in cats and cat fleas (ctenocephalides felis) under natural conditions.cats are considered the main reservoir of three zoonotic bartonella species: bartonella henselae, bartonella clarridgeiae, and bartonella koehlerae. cat fleas (ctenocephalides felis) have been experimentally demonstrated to be a competent vector of b. henselae and have been proposed as the potential vector of the two other bartonella species. previous studies have reported a lack of association between the bartonella species infection status (infected or uninfected) and/or bacteremia levels of c ...201526070666
bartonella spp. bacteremia in blood donors from campinas, brazil.bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. this pathogen can survive for over a month in stored blood. however, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. we investigated bartonella spp. prevalence in 500 blood donors from campinas, brazil, based on a cro ...201525590435
seroprevalence of antibodies against bartonella species and evaluation of risk factors and clinical signs associated with seropositivity in dogs.to determine the seroprevalence of antibodies against bartonella spp in a population of sick dogs from northern california and identify potential risk factors and clinical signs associated with seropositivity.200515900951
isolation or detection of bartonella vinsonii subspecies berkhoffii and bartonella rochalimae in the endangered island foxes (urocyon littoralis).bartonella rochalimae (b.r.) and bartonella vinsonii subsp. berkhoffii (b.v.b.) have been isolated from gray foxes (urocyon cinereoargenteus) in mainland california and high bartonella seroprevalence was reported in island foxes (u. litorralis), especially from santa cruz and santa rosa islands. as a follow-up study, the objectives were to determine the prevalence of bartonella bacteremia and seropositivity and to identify the bartonella species infecting a convenience sample of 51 island foxes ...201121802869
molecular survey of bartonella henselae and bartonella clarridgeiae in pet cats across japan by species-specific nested-pcr.cats are known to be the main reservoir for bartonella henselae and bartonella clarridgeiae, which are the agents of 'cat-scratch disease' in humans. in the present study, we investigated the prevalence of the two bartonella species on 1754 cat bloods collected from all prefectures in japan during 2007-2008 by a nested-polymerase chain reaction (pcr) targeting the 16s-23s rrna internal transcribed spacer region. overall, bartonella dna was detected in 4·6% (80/1754) of the cats examined. the nes ...201728780918
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