leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall.a new aerobic, gram-positive, nonsporulating rod-shaped organism is described: strain ifo 15245t (t = type strain) has the following characteristics: the menaquinone contains a side chain with 11 isoprenyl units; the guanine-plus-cytosine content of the dna is 66.2 mol%; 2,4-diaminobutyric acid, glutamic acid, alanine, glycine, and gamma-aminobutyric acid are present in the cell wall at a molar ratio of ca. 1:1:2:1:1; and glucose and galactose are also present in the cell wall. a comparison of p ...19968863425
[characterization and phylogenetic analysis of the new species of the leucobacter tropicalis sp. nov].a new aerobic, gram-positive, non-sporulating, rod-shaped organism is described. strain f-8 was isolated from tropical soil, and has the following characteritics: the menaquinone contains a side chain with 11 isoprenyl units(mk-11); 2,4-diaminobutyric acid, glutamic acid, alanine, glycine and a small amount of gamma-aminobutyric acid present in the cell wall at a molar ratio of 0.77:1.0:1.66:0.95. the major cellular sugar were rhamnose, galactose and glucose. the g + c content of dna is 68 mol%. ...199912555519
zimmermannella helvola gen. nov., sp. nov., zimmermannella alba sp. nov., zimmermannella bifida sp. nov., zimmermannella faecalis sp. nov. and leucobacter albus sp. nov., novel members of the family strains of actinobacteria, isolated from soil, wounds, urine, cow faeces, human blood and butter, were characterized by a polyphasic approach to clarify their taxonomic position. on the basis of chemotaxonomy, 16s rrna gene analysis and dna relatedness, strain iam 14851t can be classified within the cluster of the genus leucobacter and is proposed as a novel species, leucobacter albus sp. nov., with strain iam 14851t (=tistr 1515t) as the type strain. the other six strains formed a phyloge ...200415388726
leucobacter chromiireducens sp. nov, and leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment.two strains designated strains l-1t and l-9t were isolated from activated sludge of a treatment plant that receives wastewater from the tannery industry contaminated with chromium. phylogenetic analysis showed that the organisms represented two new species of the genus leucobacter. strains l-1t and l-9t could be distinguished from the type strain of l. komagatae and from the type strain of "l. albus" by the b-type peptidoglycan composition, fatty acid composition, several phenotypic and physiolo ...200415612621
leucobacter luti sp. nov., and leucobacter alluvii sp. nov., two new species of the genus leucobacter isolated under chromium stress.two strains designated rf6(t) and rb10(t) were isolated, from activated sludge and from river sediments, respectively, both systems receiving chromium contaminated water. phylogenetic analysis showed that strain rf6(t)and strain rb10(t) represented two new species of the genus leucobacter. strain rb10(t) can be distinguished from rf6(t) by its ability to grow at 37 degrees c, by showing a different optimum ph, by cell wall amino acids different relative amount and by having the fatty acid strait ...200616316733
characterization of microbial contamination in united states air force aviation fuel tanks.bacteria and fungi, isolated from united states air force (usaf) aviation fuel samples, were identified by gas chromatograph fatty acid methyl ester (gc-fame) profiling and 16s or 18s rrna gene sequencing. thirty-six samples from 11 geographically separated usaf bases were collected. at each base, an above-ground storage tank, a refueling truck, and an aircraft wing tank were sampled at the lowest sample point, or sump, to investigate microbial diversity and dispersion within the fuel distributi ...200616328508
leucobacter iarius sp. nov., in the family microbacteriaceae.a novel gram-positive bacterium, strain 40(t), was isolated in the course of identifying bacteria from infective juveniles of the entomopathogenic nematode steinernema thermophilum. based on 16s rrna gene analysis, strain 40(t) was found to be related to the type strains of recognized species of the genus leucobacter, family microbacteriaceae. the 16s rrna gene sequence similarity values of strain 40(t) and leucobacter albus iam 14851(t), leucobacter luti lmg 23118(t), leucobacter alluvii lmg 23 ...200717392186
comparison of in vitro cr(vi) reduction by cfes of chromate resistant bacteria isolated from chromate contaminated soil.chromate resistant and reducing strains were isolated from chromium contaminated soil and identified as bacillus sp. (kch2 and kch3), leucobacter sp. (kch4) and exiguobacterium sp. (kch5). kch3 and kch4 showed higher cr(vi) tolerance (2 mm) and cr(vi) reduction (1.5 mm) than kch5 (1.5 mm and 0.75 mm, respectively). cr(vi) reduction by cfes of kch3 and kch4 showed nad(p)h dependence, optimum activity at ph 5.5, low k(m) (45-55 microm) and substrate inhibition by cr(vi) (>75 microm), whereas that ...200817920879
microbial interactions within a cheese microbial community.the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ...200817981942
leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode caenorhabditis elegans, and emended description of l. chromiireducens.a yellow-pigmented, gram-positive, aerobic, non-motile, non-spore-forming, irregular rod-shaped bacterium (strain tan 31504(t)) was isolated from the bacteriophagous nematode caenorhabditis elegans. based on 16s rrna gene sequence similarity, dna g+c content of 69.5 mol%, 2,4-diaminobutyric acid in the cell-wall peptidoglycan, major menaquinone mk-11, abundance of anteiso- and iso-fatty acids, polar lipids diphosphatidylglycerol and phosphatidylglycerol and a number of shared biochemical charact ...200718048723
potential-ph diagram for "leucobacter sp. ch-1-cr-h(2)o" system.laboratory experiments were used to investigate the effects of initial ph and applied potential on the growth of leucobacter sp. ch-1 and cr (vi) reduction and establish the ph-eh diagram for leucobacter sp. ch-1-cr-h(2)o system. the results showed that the preferred initial ph for leucobacter sp. ch-1 growth was from 7.0 to 11.0. at this ph range, 56.2-99.5% of cr (vi) was reduced by ch-1 strain. the applied potentials from -700 to 0 mv, from -800 to +300 mv and from -800 to +400 mv at 7.0, 9.0 ...200818329801
virulence of leucobacter chromiireducens subsp. solipictus to caenorhabditis elegans: characterization of a novel host-pathogen interaction.we describe the pathogenic interaction between a newly described gram-positive bacterium, leucobacter chromiireducens subsp. solipictus strain tan 31504, and the nematode caenorhabditis elegans. tan 31504 pathogenesis on c. elegans is exerted primarily through infection of the adult nematode uterus. tan 31504 enters the uterus through the external vulval opening, and the ensuing uterine infection is strongly correlated with a significant reduction in host life span. young worms can feed and deve ...200818487405
identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens.after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ...200818650355
leucobacter tardus sp. nov., isolated from the phyllosphere of solanum tuberosum the course of studying the composition of the microbial community in the phyllosphere of potato plants, a gram-positive bacterium, strain k 70/01(t), was isolated. on the basis of 16s rrna gene sequence analysis, it was shown that the investigated isolate represents a novel species in the genus leucobacter; the closest phylogenetic neighbour was leucobacter chromiireducens subsp. chromiireducens, with a sequence similarity of 96.8 % to the type strain. chemotaxonomic characteristics supported ...200818984695
leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass.a gram-positive, rod-shaped, aerobic, non-motile, yellow-pigmented bacterial strain, designated mm2lb(t), was isolated from a chironomid egg mass that was sampled in a waste stabilization pond in northern israel. 16s rrna gene sequence analysis indicated that the isolate should be placed in the genus leucobacter (family microbacteriaceae). levels of 16s rrna gene sequence similarity between strain mm2lb(t) and the type strains of recognized leucobacter species were 97.1 % or lower. the dominant ...200919329584
assessment of the microbial diversity at the surface of livarot cheese using culture-dependent and independent approaches.the microbial diversity of the surface of a commercial red-smear cheese, livarot cheese, sold on the retail market was studied using culture-dependent and independent approaches. forty yeasts and 40 bacteria from the cheese surface were collected, dereplicated using single-strand conformation polymorphism (sscp) analysis and identified using rrna gene sequencing for the culture-dependent approach. the culture-independent approach involved cloning and sequencing of the 16s rrna gene and sscp anal ...200919481828
leucobacter aerolatus sp. nov., from the air of a duck barn.a gram-positive, non-spore-forming actinobacterium (sj 10(t)) was isolated on tryptone soy agar from the air of a duck barn after filter sampling. based on 16s rrna gene sequence similarity studies, strain sj 10(t) was shown to belong to the genus leucobacter and was closely related to leucobacter chromiireducens subsp. chromiireducens l-1(t) (97.8 %), leucobacter tardus dsm 19811(t) (97.3 %) and leucobacter luti rf6(t) (97.3 %). the peptidoglycan of strain sj 10(t) contained 2,4-diaminobutyric ...201020081017
leucobacter salsicius sp. nov., from a salt-fermented food.strain m1-8(t) was isolated from jeotgal, a korean salt-fermented food. cells were aerobic, non-motile, gram-reaction-positive and rod-shaped. colonies were cream-coloured and circular with entire margins. strain m1-8(t) exhibited optimal growth at 25-30 °c and ph 7.0-8.0 and in 0-4  % (w/v) nacl. the strain tolerated up to 10.0 mm cr(vi). phylogenetic analyses of 16s rrna gene sequences indicated that strain m1-8(t) represents a novel species in the genus leucobacter. the 16s rrna gene sequence ...201120363927
leucobacter chromiiresistens sp. nov., a chromate-resistant strain.a gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain jg 31(t), was isolated in the course of identifying chromium-resistant soil bacteria. 16s rrna gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus leucobacter. binary 16s rrna gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97 % with leucobacter allu ...201120511468
[the midgut bacterial flora in lab-reared anopheles sinensis].to screen gram-negative bacteria in the midguts of lab-reared anopheles sinensis at 3 development stages (larva, unfed adult and engorged adult).201020666322
diversity and abundance of oil-degrading bacteria and alkane hydroxylase (alkb) genes in the subtropical seawater of xiamen this report, the diversity of oil-degrading bacteria and alkb gene was surveyed in the seawater around xiamen island. forty-four isolates unique in 16s rrna sequence were obtained after enrichment with crude oil. most of the obtained isolates exhibited growth with diesel oil and crude oil. alkb genes were positively detected in 16 isolates by degenerate polymerase chain reaction (pcr). and for the first time, alkb genes were found in bacteria of gallaecimonas, castellaniella, paracoccus, and ...201020683589
leucobacter celer sp. nov., isolated from korean fermented seafood.a novel, gram-positive, aerobic, rod-shaped, and non-motile bacterial strain, nal101(t), was isolated from gajami-sikhae, a traditional korean fermented seafood made of flatfish. growth occurred at 4-45°c and ph 5-10, and in 0-12 % (w/v) nacl. optimum growth occurred at 30-37°c and ph 8, and in 0-1 % (w/v) nacl. the cell wall amino acids were 2,4-diaminobutyric acid, alanine, glycine, threonine, and glutamic acid, and the major fatty acids were anteiso-c(15:0), iso-c(16:0), and anteiso-c(17:0). ...201021037031
surface properties of bacteria from activated sludge in relation to bioflocculation.two bacterial stains were isolated from the activated sludge and identified as leucobacter sp. and alcaligenesfaecalis by 16s rdna sequencing. pure cultures of these two strains, representing well or poorly settled bacteria, were used to investigate the mechanism of bioflocculation in activated sludge. based on the analyses of the characteristics of cells hydrophobicity, zeta-potential, flocculation ability and extracellular polymeric substance (eps) composition under different growth stages, it ...201021462699
leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost.a gram-positive bacterium, strain k-540b(t), which was separated from a mixed culture isolated from compost, was subjected to a polyphasic taxonomic study. strain k-540b(t) was found to have 95.5%-98.7% sequence similarity with the genus leucobacter based on 16s rrna gene sequence analyis. phenotypic and chemical properties of strain k-540b(t) were consistent with its classification in the genus leucobacter. the major quinone system compound was mk-11 and major fatty acids were anteiso-c(15:0) a ...201121478645
identification and characterization of leucobacter sp. n-4 for ni (ii) biosorption by response surface the present study, batch experiments were carried out to characterize and optimize the removal process of ni (ii) by a nickel tolerant strain leucobacter sp. n-4, which was isolated from the soil samples. the effects of operating parameters with respect to initial solution ph (3.0-6.5), initial nickel concentration (50-100mg/l) and biomass dosage (1-10g/l) on ni (ii) biosorption were investigated by response surface methodology (rsm). the maximal ni (ii) removal efficiency (nearly 99%) was ac ...201121531505
surface microbial consortia from livarot, a french smear-ripened cheese.the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ...201121815832
Leucobacter kyeonggiensis sp. nov., a new species isolated from dye waste water.A Gram-positive, aerobic, non-motile bacterium designated F3-P9(T), was isolated from dye waste water in Korea and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain F3-P9(T) belongs to genus Leucobacter. The 16S rRNA gene sequence similarities among strain F3-P9(T) and validated representatives of the genus Leucobacter ranged from 95.9-97.4%. Strain F3-P9(T) exhibited DNA-DNA relatedness values below 48% with respect to Leucob ...201122203572
draft genome sequence of leucobacter chromiiresistens, an extremely chromium-tolerant we present the draft genome of leucobacter chromiiresistens. this is the first genome sequence of an organism belonging to the genus leucobacter. l. chromiiresistens was sequenced due to its capability to tolerate up to 300 mm cr(vi) in the medium, which is so far a unique feature for microorganisms.201222207746
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