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occurrence of faecal bacteria, salmonella and antigens associated with hepatitis a virus in shellfish.an investigation was carried out over a one year period to examine jointly the occurrence of faecal bacteria, salmonella and the presence of antigens associated with the hepatitis a virus (hav) in oysters (crassostrea gigas), mussels (mytilus edulis, mytilus galloprovincialis) and cockles (cerastoderma edule), taken from 8 shellfish farming areas or natural beds along the french coast. for the faecal coliforms (fc) and faecal streptococci (fs), statistical analysis of the 176 samples examined sh ...19911663750
transfer across the human gut of environmental plutonium, americium, cobalt, caesium and technetium: studies with cockles (cerastoderma edule) from the irish sea.our previous studies have indicated lower values of the gut transfer factor ('f1 values') for plutonium and americium in winkles (littorina littorea) than adopted by icrp. the present study was undertaken primarily to investigate whether this observation extends to other species. samples of cockles (carastoderma edule) from ravenglass, cumbria were eaten by volunteers who provided 24 h samples of urine and faeces. urine samples indicated f1 values for cockles which were higher than for winkles; ...19989656190
ultrastructural studies on the tegument of the metacercariae of meiogymnophallus minutus and meiogymnophallus fossarum (digenea: gymnophallidae) in cerastoderma edule (bivalvia) from portugal.unencysted metacercariae of meiogymnophallus minutus (cobbold, 1859) and the sibling species meiogymnophallus fossarum (bartoli, 1965) infect the edible cockle, cerastoderma edule (l.), with free metacercariae occurring simultaneously in the same host specimens in portugal. observations on the tegument of both species show that changes in the morphology of the body surface occurs in the intermediate host. scanning and transmission electron microscopy revealed an alveoli-like tegument with palmat ...19989714200
bioaccumulation of mycotoxins by shellfish: contamination of mussels by metabolites of a trichoderma koningii strain isolated in the marine environment.to determine whether toxic metabolites produced by fungi could cause shellfish toxicities, mussels were contaminated in laboratory conditions by sterile filtrates of a liquid culture of a strain of the fungus trichoderma koningii previously isolated from a shellfish, the cockle (cerastoderma edule). mussels were kept in aerated natural seawater and fed with a culture of the microalga isochrysis galbana, to which a filtrate of liquid fungal culture was added. mussels were exposed to contamination ...19999920482
detection of infectious cryptosporidium parvum oocysts in mussels (mytilus galloprovincialis) and cockles (cerastoderma edule).infective cryptosporidium parvum oocysts were detected in mussels (mytilus galloprovincialis) and cockles (cerastoderma edule) from a shellfish-producing region (gallaecia, northwest spain, bounded by the atlantic ocean) that accounts for the majority of european shellfish production. shellfish were collected from bay sites with different degrees of organic pollution. shellfish harboring c. parvum oocysts were recovered only from areas located near the mouths of rivers with a high density of gra ...200010788352
the life cycle of monorchis parvus (digenea: monorchiidae) demonstrated by developmental and molecular data.cercaria cerastodermae i, a digenean parasite of cerastoderma edule, was recorded for the first time in the atlantic ocean off the iberian peninsula. sporocysts were present in the hemolymph of the digestive gland, gonad, gills, and foot of the mollusc. most of the cercariae present within sporocysts were encysted as metacercariae. the corresponding adult stages were obtained after experimental infection of several diplodus sargus artificially reared in fish farms and that had previously been pr ...200010864243
acute and acclimated digestive responses of the cockle cerastoderma edule (l.) to changes in food quality and quantity. i. feeding and absorption of biochemical components.cockles cerastoderma edule were fed two different concentrations ( approximately 0.8 and 2 mm(3) l(-1)) of two diets with different qualities ( approximately 10 and 60% of organic content) which were achieved by mixing different proportions of ashed silt particles with cells of the microalgae tetraselmis suecica. clearance, ingestion and absorption rates of organic matter and biochemical components were measured after 3 days (acute response) and 11 days (acclimated response) of exposure to the d ...200010967333
acute and acclimated digestive responses of the cockle cerastoderma edule (l.) to changes in the food quality and quantity. ii. enzymatic, cellular and tissular responses of the digestive gland.cockles cerastoderma edule were fed two different concentrations of two diets with different qualities which were achieved by mixing different proportions of ashed silt particles with cells of the microalgae tetraselmis suecica. after 3 days (acute response) and 11 days (acclimated response) of exposure to the diets, we analysed the digestive activity of the digestive gland using cyto-histological and enzymatic techniques. we measured (i) the volumetric fraction of digestive and basophilic cells ...200010967334
disseminated neoplasia and large foci indicating heavy haemocytic infiltration in cockles cerastoderma edule from galicia (nw spain).a histopathological survey was performed to search for the cause of high mortality in the cockle cerastoderma edule in northern rias of galicia (northwestern spain). from limited sampling, 2 severe pathological conditions with high prevalence were found: disseminated neoplasia and the occurrence of numerous, large foci of heavy haemocytic infiltration. both conditions were detected in areas affected by high mortality and were absent (foci of haemocytic infiltration) or with lower prevalence (dis ...200111710555
parasites and pathologic conditions of the cockle cerastoderma edule populations of the coast of galicia (nw spain).a survey of pathological conditions affecting cockle populations of the most economically important natural beds of galician estuaries in nw spain was performed. samples of 30 adult cockles were collected from each of 34 natural beds in the spring of 1999 and processed by histological techniques. disseminated neoplasia were seen in samples from most of the natural beds, in some cases with a high prevalence. the gregarine nematopsis sp., larval trematode stages, and branchial extracellular large ...200111812111
pectenotoxin-2 seco acid, 7-epi-pectenotoxin-2 seco acid and pectenotoxin-2 in shellfish and plankton from portugal.pectenotoxin-2 seco acid (ptx2sa) and 7-epi-pectenotoxin-2 seco acid (7-epi-ptx2sa) were found in portuguese shellfish both by fluorescence detection after adam derivatisation and, liquid chromatography coupled with mass spectrometry detection. two time-series both with blue mussel (mytilus edulis) and common cockle (cerastoderma edule) from aveiro lagoon illustrate how ptx2sa has a strong association with dinophysis acuta occurrence in the plankton, as well as dinophysis fortii. data so far exc ...200212076652
cockle emergence at the sediment surface: 'favourization' mechanism by digenean parasites?the aim of the present work was to assess the effect of digenean trematodes on indirect mortality of the cockle cerastoderma edule, an infaunal bivalve. the tested hypothesis was that parasites altered the burrowing capacity of cockles and thus exposed them at the sediment surface, where they are more vulnerable to predators. if the predator is the final host, this mechanism, which drives the cockle out of the sediment, is considered as a 'favourization'. cockle populations from 2 stations in ar ...200212542091
virus-like particles associated with large foci of heavy hemocytic infiltration in cockles cerastoderma edule from galicia (nw spain). 200314726246
infection of the cockle cerastoderma edule in the baie des veys (france) by the microsporidian parasite steinhausia sp.we report the occurrence of the microsporidian parasite steinhausia sp. in the oocytes of the common cockle cerastoderma edule in a natural population in france, where high mortalities occurred. steinhausia sp. appeared primarily as sporocysts containing many small spores, and putative earlier developmental stages were also observed. both its prevalence and infection intensity were low, and no host defence reaction was recognized, suggesting that steinhausia sp. had no detrimental effect on c. e ...200314735931
interspecific variation of metal concentrations in three bivalve mollusks from galicia.there has been growing concern about the inflow of metals to the coastal areas because they can be toxic to aquatic and human life. some studies have demonstrated the existence of species-specific differences in the metal concentrations of mollusks. we compared metal concentrations between mytilus galloprovincialis, used as a water quality indicator, and two other bivalve species collected for human consumption (venerupis pullastra and cerastoderma edule) in different locations on the galician c ...200415386128
geochemical survey and metal bioaccumulation of three bivalve species (crassostrea gigas, cerastoderma edule and ruditapes philippinarum) in the nord medoc salt marshes (gironde estuary, france).a 15-month experiment combining a geochemical survey of cd, cu, zn and hg with a bioaccumulation study for three filter-feeding bivalve species (oysters, crassostrea gigas; cockles, cerastoderma edule; and clams, ruditapes philippinarum) was conducted in a breeding basin of the nord medoc salt marshes connected to the gironde estuary, which is affected by historic polymetallic pollution. regular manual surface measurements of temperature, salinity, ph and dissolved o(2) concentration and hourly ...200515626396
proteomic approach to probe for larval proteins of the mussel mytilus galloprovincialis.a proteomic approach was used to search for larval proteins specific to the mussel mytilus galloprovincialis from galicia in northwest spain. the study included both a comparative analysis, through two-dimensional electrophoresis, of protein expression maps of the larvae of the mussel and of 5 abundant and commercially important bivalve species from the region (ostrea edulis, cerastoderma edule, pecten maximus, tapes decussatus, and venarupis pullastra) and subsequent mass spectrometric analysis ...200515976943
urosporidium sp. hyperparasite of the turbellarian paravortex cardii in the cockle cerastoderma edule.cysts with spores showing different degree of maturity and a single plasmodium were observed in the connective tissue of the turbellarian paravortex cardii located in the digestive lumen of the cockle cerastoderma edule. the study of spore morphology by transmission electron microscopy revealed that they correspond to an haplosporidian belonging to the genus urosporidium. spore ornaments were similar to those described from urosporidium spisuli, infecting a nematode parasite of the atlantic surf ...200516214165
distribution of aliphatic compounds in bivalve mollusks from galicia after the prestige oil spill: spatial and temporal trends.the content and distribution of n-alkane (c8-c35) and isoprenoid (pristane and phytane) hydrocarbons were investigated in two species of bivalve mollusk, mussel (mytilus galloprovincialis) and cockle (cerastoderma edule), collected at different points of the galicia littoral zone during the period from december 2002 to february 2003 (after the prestige oil spill). samples were analyzed by high-resolution gas chromatography equipped with a flame ionization detector. the highest levels were found ...200616253223
impact of digenean parasite infection on metallothionein synthesis by the cockle (cerastoderma edule): a multivariate field monitoring.metallothioneins (mt) are proteins that play an important role in metabolism of essential metals and detoxification of trace metals from living organisms. their synthesis is induced by metal pollution but can also be exacerbated by other factors such as reproduction processes. in this context, we monitored mt concentrations in a cockle cerastoderma edule (marine bivalve) population and highlighted the effect of a castrating digenean parasite, labratrema minimus. in spent cockles, mt levels were ...200616257015
[structure and bioassessment of benthic communities of a lagoonal ecosystem of the atlantic moroccan coast].the merja zerga lagoon is a semi-enclosed marine ecosystem in which various types of human activities have been developed. this paper characterizes the biosedimentary units of the lagoon and defines a reference status of the quality and health of the macrozoobenthic communities that can be used as bioindicators of the quality of the global marine environment. specific and functional diversity were high: 147 taxa were identified; they were distributed within seven main trophic groups. trophic str ...200516286087
differential dynamics of dinophysistoxins and pectenotoxins, part ii: offshore bivalve species.different dinophysistoxin's profiles have been found repeatedly amongst some offshore bivalve molluscs. species such as the clam, spisula solida, esterify dinophysistoxins to a great extent, and contain always more okadaic acid (oa) than dinophysistoxin-2 (dtx2). in contrast, the clam donax trunculus has a much higher percentage of non-esterified toxins, and often contains more dtx2 than oa. a detoxification experiment with d. trunculus and s. solida showed that the higher percentage of dtx2 in ...200616321414
flow cytometric dna content analysis of neoplastic cells in haemolymph of the cockle cerastoderma edule.epizootiological outbreaks of disseminated neoplasia (dn) have been reported in association with mass mortalities in various bivalve species including the cockle cerastoderma edule. a flow cytometric (fcm) procedure to study dna content was successfully adapted and tested in haemolymph cells (haemocytes and neoplastic cells) of the cockle. the fcm results were similar to those obtained by histological analysis (dn diagnosis and haemolymph cell features). fcm analysis revealed differences in dna ...200516385819
molecular fingerprinting of vibrio tapetis strains using three pcr-based methods: eric-pcr, rep-pcr and rapd.brown ring disease (brd) is a bacterial disease caused by vibrio tapetis which affects cultured clams and causes heavy economic losses. in this study, 28 v. tapetis strains isolated from 5 different hosts were intraspecifically characterized by 3 different polymerase chain reaction- (pcr-) based typing methods: enterobacteria repetitive intergenic consensus (eric)-pcr, repetitive extragenic palindromic (rep)-pcr and randomly amplified polymorphic dna (rapd)-pcr. cluster analysis of genetic profi ...200616724561
digenean larvae parasitizing cerastoderma edule (bivalvia) and nassarius reticulatus (gastropoda) from ria de aveiro, portugal.this work reviews the significant community of digenean trematodes observed infecting the edible cockle, cerastoderma edule, and the snail nassarius reticulatus in the channel of s. jacinto, ria de aveiro estuary, portugal. the digenea observed comprise 15 species, belonging to the families bucephallidae (1), echinostomatidae (4), gymnophallidae (3), lepocreadiidae (1), microphallidae (1), monorchiidae (1), renicolidae (1), strigeidae, (1), and zoogonidae (2). the species were studied by light m ...200616729689
first record and description of metacercariae of curtuteria arguinae n. sp. (digenea: echinostomatidae), parasite of cockles cerastoderma edule (mollusca: bivalvia) in arcachon bay, france.a new himasthlinae species, curtuteria arguinae, is described as metacercariae from the cockle cerastoderma edule (l.), collected at banc d'arguin (southwestern france). these metacercariae encysted preferentially in the mantle and also in the foot of cockles. encysted and chemically excysted metacercariae were studied by light microscopy and scanning electron microscopy, respectively. excysted metacercariae were elongated and curved ventrally. they bore a 33-spine circumoral collar. sensory pap ...200616884002
mercury content in tinned molluscs (mussel, cockle, variegated scallop, and razor shell) normally consumed in spain, 2005.concentrations of mercury were determined for tinned molluscs (mollusca, bivalvia), i.e., mussels (mytilus spp.), cockles (cerastoderma edule), variegated scallops (chlamys varia), and razor shells (ensis spp.), consumed in spain. a total of 220 samples were analyzed: 120 mussels, 40 cockles, 24 variegated scallops, and 36 razor shells. samples were obtained weekly from markets in santa cruz de tenerife (canary islands) over a period of 12 months. all observed concentrations of mercury were belo ...200616995530
evidence of an altered protective effect of metallothioneins after cadmium exposure in the digenean parasite-infected cockle (cerastoderma edule).the aim of the present study was to analyse the relation between parasitism and subsequent metallothioneins (mt) in the case of metal contamination. experimental exposure of parasitized and unparasitized cockles (cerastoderma edule) to cadmium (cd) was performed, with the cockle as first or second intermediate host of 2 digenean species. after 7 days of cd exposure in microcosms, cockles infected as first intermediate host by labratrema minimus exhibited metal concentrations in tissues double th ...200717032474
habitat and transmission--effect of tidal level and upstream host density on metacercarial load in an intertidal bivalve.transmission of parasites may be mediated by their habitat, consisting of abiotic and biotic components. i investigated the effect of 2 important habitat components in intertidal ecosystems, tidal level (abiotic) and density of upstream hosts (biotic), on the transmission of trematode cercariae to cockle (cerastoderma edule) hosts. a field survey showed no general trend in metacercarial loads of cockles regarding tidal level but species-dependent reactions. parasites originating from littorina l ...200717076926
evidence of retroviral etiology for disseminated neoplasia in cockles (cerastoderma edule).epizootiologic outbreaks of disseminated neoplasia have been reported in association with massive mortalities of various bivalve species. in cockles, cerastoderma edule, this pathological condition was described in ireland and france. since 1997, different populations affected by this pathology have been detected in galicia (nw spain). transmission electron microscopy allowed the visualization of virus-like particles in neoplastic cells, resembling a retrovirus-like agent. to confirm this hypoth ...200717092515
shellfish dredging pushes a flexible avian top predator out of a marine protected area.there is a widespread concern about the direct and indirect effects of industrial fisheries; this concern is particularly pertinent for so-called "marine protected areas" (mpas), which should be safeguarded by national and international law. the intertidal flats of the dutch wadden sea are a state nature monument and are protected under the ramsar convention and the european union's habitat and birds directives. until 2004, the dutch government granted permission for ~75% of the intertidal flats ...200617105350
development and validation of a rapid headspace gas chromatography-mass spectrometry method for the determination of diethyl ether and acetone residues in tween extracts of shellfish intended for mouse bioassay for diarrhoetic toxins.a validated analytical method using headspace capillary gas chromatography with mass spectrometric detection, which utilises deuterated analogues of the target analytes as internal standards has been developed and applied to the determination of acetone and diethyl ether in tween extracts of cockles (cerastoderma edule) and mussels (mytilus edulis) destined for mouse bioassay for lipophilic toxins. the optimal conditions for headspace incubation were 50 degrees c for 6 min. the limits of detecti ...200717499755
effects of body-size and season on digestive organ size and the energy balance of cockles fed with a constant diet of phytoplankton.seasonal variation in size-dependence of seawater clearance rate, absorption efficiency, oxygen consumption, gill area, length of the crystalline style and dry weight of digestive gland was analyzed in cockles cerastoderma edule from the mundaka estuary, spain. experimental determinations were performed monthly (from july 1998 to november 1999) in cockles being fed with tetraselmis suecica (organic content: 87.84 +/- 1.95%) at a concentration of 3 mm(3)/l for 3 days. analysis of covariance revea ...200818193430
the human alimentary tract transfer and body retention of environmental polonium-210.this paper presents the results of a 4 year study to investigate the human alimentary tract transfer factor (f(a) value) and body retention of 210 po in shellfish. in the first 3 years, mussels (mytilus edulis l.), cockles (cerastoderma edule l.) and brown meat from crab (cancer pagurus l.) were successively studied. in each year five volunteers (from a pool of seven) ate a suitable portion of the shellfish and provided 24 h samples of excreta usually for 3 days before and for at least 7 days du ...200718268372
confirmation by lc-ms/ms of azaspiracids in shellfish from the portuguese north-western coast.the search for azaspiracids (azas) in shellfish on the portuguese coast started in 2002, but the presence of these toxins could not be demonstrated until the summer of 2006. analysis by liquid-chromatography-tandem mass spectrometry (lc-ms/ms) allowed the confirmation of aza2 as a dominant compound, followed by aza1, in blue mussel (mytilus galloprovincialis), common cockle (cerastoderma edule), clams (venerupis senegalensis, ruditapes decussatus), razor clam (solen marginatus) and oyster (crass ...200818471847
identification and description of bucephalus minimus (digenea: bucephalidae) life cycle in portugal: morphological, histopathological, and molecular data.the cercaria of bucephalus minimus infects the digestive gland and gonads of its first intermediate host, the edible cockle, cerastoderma edule. light microscopy (lm) and scanning electron microscopy (sem) of the cercaria showed a tail formed by a central stem, with 2 long contractile arms presenting distinct morphological surfaces. the encysted metacercaria naturally infected the flathead grey mullet, mugil cephalus. the cysts found in the heart, liver, and spleen were shown to be identical by ...200918710298
the macrobenthic community along a mercury contamination in a temperate estuarine system (ria de aveiro, portugal).mercury is a widely distributed environmental pollutant and a toxic element to all living organisms. this study represents an attempt to evaluate its correlation with the macrobenthic community structure in a temperate estuarine system, the ria de aveiro (portugal). the macrobenthic community structure showed significant differences along the mercury gradient. overall, the increase of mercury contamination was associated with reduced total abundance, lower species richness, and dominance of tole ...200818765161
oxidative stress responses in bivalves (scrobicularia plana, cerastoderma edule) from the oued souss estuary (morocco).the aim of this work was to study the responses of oxidative-stress biomarkers (catalase: cat, thiobarbituric acid reactive substances: tbars) in bivalves (scrobicularia plana and cerastoderma edule) from the oued souss estuary (morocco). this estuary, subjected to large amounts of sewage discharges and industrial effluents, do not receive since november 2002 any waste outlets due to their connection to a wastewater purification plant. high cat and tbars values were recorded in both species coll ...200918995904
morphological and molecular studies on life cycle stages of diphtherostomum brusinae (digenea: zoogonidae) from northern portugal.diphtherostomum brusinae was first recorded by the present study in the north of portugal. sporocysts, containing cercariae and encysted metacercariae, were observed in the gonads and digestive gland of the gastropod nassarius reticulatus. metacercariae were also found infecting the foot, mantle border and gills of the cockle cerastoderma edule. the adult form was lodged in the rectum of the definitive host diplodus sargus. the morphology of the three parasitic stages was studied by light (lm) a ...200919265558
digenean trematodes-marine mollusc relationships: a stable isotope study.the stable carbon and nitrogen isotopic composition of digenean trematode parasites and their marine mollusc hosts was investigated to describe the potential influence of parasites on their host and its different tissues, and to obtain further insight into their trophic relationships. four parasite-host systems were studied: labratrema minimus-cerastoderma edule, monorchis parvus-c. edule, lepocreadiidae parasites-nassarius reticulatus and zoogonidae parasites-n. reticulatus. among the 4 samplin ...200919419008
diopatra neapolitana (polychaeta: onuphidae) as a second intermediate host of gymnophallus choledochus (digenea: gymnophallidae) in the aveiro estuary (portugal): distribution within the host and histopathology.this is the first host record of gymnophallus choledochus metacercariae infecting the polychaete diopatra neapolitana in the aveiro estuary (portugal). the metacercariae were found unencysted, and their morphology is similar to that presented by metacercariae harbored by nereis diversicolor and cerastoderma edule, although they are larger in size. in d. neapolitana, the prevalence and mean intensity of the infection was very high, at 100% and 202 +/- 139 metacercariae per host, respectively, sug ...200919441876
metabolites of saxitoxin analogues in bivalves contaminated by gymnodinium catenatum.bivalve metabolites of saxitoxin analogues, not present in microalgae, were recently described as an important toxin fraction in mussels contaminated by alexandrium tamarense. these possess very low fluorescence, and require mass spectrometry detection. hilic-ms was implemented to look for these metabolites in bivalves contaminated during gymnodinium catenatum blooms at the portuguese coast. the presence of m1 was tentatively identified in several bivalves, ranging from estuarine (mytilus gallop ...201019622366
brominated dioxins (pbdd/fs) and pbdes in marine shellfish in the uk.the occurrence of brominated dioxins (pbdd/fs) and polybrominated diphenyl ethers (pbdes) was investigated in commonly consumed species of marine shellfish in the uk. individual samples of pacific oysters (crassostrea gigas), native oysters (ostrea edulis), mussels (mytilus edulis), scallops (pecten maximus), and cockles (cerastoderma edule) were collected from different coastal regions between 2006 and 2007. samples of a particular species from each site were composited and 60 samples were anal ...200919680967
effect of some habitual cooking processes on the domoic acid concentration in the cockle (cerastoderma edule) and manila clam (ruditapes philippinarum).the effect of cooking on the concentration and burden of domoic acid in two bivalve molluscs was studied. the manila clam (ruditapes philippinarum) and cockle (cerastoderma edule) were subjected to steaming and boiling, respectively. in both cases, factorial plans were used to evaluate the effects of common cooking methods and the variations likely to take place during the cooking procedure (cooking time and salt concentration in both species, in addition to ethanol percentage in manila clam). t ...200919680984
interactive effects of metal contamination and pathogenic organisms on the marine bivalve cerastoderma edule.the present study evaluated the interactive effects of cadmium contamination and pathogenic organisms (trematodes himasthla elongata and bacteria vibrio tapetis) singularly and in combination during 7 days on the bivalve cerastoderma edule. some defense-related activities were analyzed such as genetic expression, metallothionein and immune responses. trematode metacercarial infection, similar whatever the treatment, induced the strongest responses of immune parameters. particularly, the interact ...201020031177
autochthonous clams monitoring of ganzirri lake (sicily).two autochthonous clams, venerupis aurea laeta and cerastoderma edule/glaucum, collected for a period of 1 year, from may 2007 to january 2008, were studied in the cape peloro lagoon. on the samples, it was evaluated the presence of pathogenic microorganisms and proteins, lipids and yield edible part were determined. it was evaluated the concentration of mineral elements (cd, cu, mn, se, pb, zn) and organic contaminants (∑ddt and pcbs). salmonella is never present in any sample. total and faecal ...201020033279
genotyping of hepatitis a virus detected in bivalve shellfish in galicia (nw spain).hepatitis a virus (hav) represents a significant public health problem due to its high persistence in the environment and its transmission through contaminated water and food. bivalve shellfish are filter feeders that can bioaccumulate human pathogens found in contaminated waters, their consumption being a potential cause of hepatitis a outbreaks. in this work, cultured and wild bivalve shellfish from the ría de vigo (galicia, nw spain) were analysed for the presence and genotyping of hav. a tot ...201020057087
prevalence, intensity, and aneuploidy patterns of disseminated neoplasia in cockles (cerastoderma edule) from arcachon bay: seasonal variation and position in sediment.the present report presents the first evidence of disseminated neoplasia (dn) in cockles cerastoderma edule from arcachon bay (france). aneuploidy of neoplastic cells allowed the use of flow cytometry (fcm) to diagnose and stage dn. a 1year survey (2007) of the prevalence and intensity (% of aneuploid circulating cells in neoplastic cockles) was conducted. prevalences ranged from 2.2% (june) to 13.6% (may), and disease intensity ranged from 18.7% (june) to 95.5% (september). these percentages we ...201020159021
morphological and molecular analysis of metacercariae of diphtherostomum brusinae (stossich, 1888) stossich, 1903 from a new bivalve host mytilus galloprovincialis.the digenean trematode diphtherostomum brusinae (stossich, 1888) stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. the present study represents the first description of the metacercariae from d. brusinae infecting the labial palps of a new intermediate host, mytilus galloprovincialis, in the aveiro estuary, portugal. the morphology of this parasitic stage was studied by light (lm) and scanning electron microscopy (sem) and some differences were report ...201020701832
expression of mutant protein p53 and hsp70 and hsp90 chaperones in cockles cerastoderma edule affected by neoplasia.high prevalence of disseminated neoplasia has been found in cockles cerastoderma edule of galicia (nw spain). disseminated neoplasia has been associated with high mortalities of various bivalve species. in vertebrates, proteins such as p53 and heat shock proteins (hsps) play important roles in carcinogenesis. the protein p53 has been detected in neoplastic cells of bivalve molluscs such as mytilus edulis, mytilus trossulus, mya arenaria, spisula solidissima, crassostrea rhizophorae and crassostr ...201020815330
source and impact of lead contamination on δ-aminolevulinic acid dehydratase activity in several marine bivalve species along the gulf of cadiz.coastal areas and estuaries are particularly sensitive to metal contamination from anthropogenic sources and in the last few decades the study of space-time distribution and variation of metals has been extensively researched. the gulf of cadiz is no exception, with several rivers draining one of the largest concentrations of sulphide deposits in the world, the iberian pyrite belt (ipb). of these rivers, the guadiana, one of the most important in the iberian peninsula, together with smaller rive ...201020965582
comparison of antibacterial activity in the hemolymph of marine bivalves from galicia (nw spain).a screening study of in vitro antibacterial activity was conducted in marine bivalves with economical importance and widespread along the coast of galicia (nw spain). hemocyte lysate supernatant (hls) and plasma of mytilus galloprovincialis, ostrea edulis, crassostrea gigas, ruditapes decussatus, ruditapes philippinarum, and cerastoderma edule were incubated with vibrio splendidus and micrococcus sp. hls and plasma for all the species demonstrated antibacterial activity, and c. edule had the hig ...201021134379
health concerns of consuming cockles (cerastoderma edule l.) from a low contaminated coastal system.commercial and recreational harvesting of shellfish within the coastal systems is usually very extensive. since these ecosystems are frequently subjected to contamination, namely from agricultural, urban and industrial activities, and shellfish generally display a high capacity to bioaccumulate metals, populations may be at risk in terms of toxic metal exposure as a consequence of the harvesting and ingestion of near shore coastal marine organisms. shellfish is regularly tested for concentration ...201121507485
norovirus, hepatitis a virus and enterovirus presence in shellfish from high quality harvesting areas in portugal.this is the first report on the screening of shellfish from portugal for the presence of human enteropathogenic viruses. approximately 2000 shellfish (curbicula fluminea, ruditapes decussatus, tellina crassa, spisula solida, dosinia exoleta, ensis spp., mytilus spp., ostrea edulis and cerastoderma edule), organized in 49 batches, were collected between march 2008 and february 2009. they were tested for norovirus (nov), hepatitis a virus (hav) and enterovirus (ev) by rt-pcr followed by nucleotide ...201121569936
dynamics of bacterial communities in cockles (cerastoderma edule) with respect to trematode parasite (bucephalus minimus) infestation.the bacterial communities associated with the cockle (cerastoderma edule) were investigated at the individual level through a 10-month monitoring programme. temporal changes and those changes associated with a common parasite of the cockle, bucephalus minimus, were investigated by monthly sampling of individuals, selected based on their shell length (cohort monitoring). cockle bacterial community abundance (cbca) and diversity (cbcd) were estimated by epifluorescence microscopy counts and automa ...201121584755
Looking for suitable biomarkers in benthic macroinvertebrates inhabiting coastal areas with low metal contamination: comparison between the bivalve Cerastoderma edule and the Polychaete Diopatra neapolitana.Metals accumulated in marine sediments are often a threat to benthic communities. With the recognized importance and wide use of stress biochemical responses as indicators of metal contamination it becomes essential to compare these markers between different species and verify their ubiquity and accuracy. Using wild Diopatra neapolitana and Cerastoderma edule, collected at several areas differing in metal contamination, this study aimed to assess the use of these two macrobenthic species as sent ...201221890202
quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography-mass spectrometry: inter-laboratory validation study.thirteen laboratories participated in an inter-laboratory study to evaluate the method performance characteristics of a liquid chromatography-tandem mass spectrometric method (lc-ms/ms) for marine lipophilic shellfish toxins. method performance characteristics were evaluated for mussel (mytilus edulis), oyster (crassostrea gigas) and cockle (cerastoderma edule) matrices. the specific toxin analogues tested included okadaic acid (oa), dinophysistoxins-1 and -2 (dtx1, -2), azaspiracids-1, -2 and - ...201121995816
biological availability of sediment-bound cadmium to the edible cockle, cerastoderma edule. 2004519047
review: bucephalus minimus, a deleterious trematode parasite of cockles cerastoderma spp.trematodes are the most prevalent and abundant macroparasites in coastal waters. they display a complex life cycle with alternation of free-living and parasitic stages generally involving three host species. the most deleterious stage is in the first intermediate host (a mollusc) where the parasite penetrates as miracidium larvae and asexually multiplicates in sporocysts/rediae to provide cercariae larvae. however, due to basic low prevalence in ecosystems, this system remains difficult to study ...201525681142
application of phage therapy during bivalve depuration improves escherichia coli decontamination.the present study investigated the potential application of the bacteriophage (or phage) pht4a, eca2 and the phage cocktail pht4a/eca2 to decrease the concentration of escherichia coli during the depuration of natural and artificially contaminated cockles. depuration in static seawater at multiplicity of infection (moi) of 1 with single phage suspensions of pht4a and eca2 was the best condition, as it decreased by ∼2.0 log cfu/g the concentration of e. coli in artificially contaminated cockles a ...201727697159
detection and quantification of hepatitis a virus and norovirus in spanish authorized shellfish harvesting areas.an 18-month survey was conducted in ten class "b" harvesting areas from two galician rias (nw of spain), the most important bivalve production area in europe, to determine the prevalence of hepatitis a virus (hav) and human norovirus (nov), including genogroups i (gi) and ii (gii). quantification was performed by reverse transcription real-time pcr (rt-qpcr), according to the recently developed standard method iso/ts 15216-1:2013. four bivalve species were studied, including wild and cultured mu ...201525462922
perkinsus chesapeaki observed in a new host, the european common edible cockle cerastoderma edule, in the spanish mediterranean coast.histological observations showed the presence of a perkinsus sp. parasite in cerastoderma edule tissues for the first time in the spanish mediterranean coast. its molecular characterization by pcr-rflp, in situ hybridization and sequencing, identified the parasite as perkinsus chesapeaki, with a maximum identity of 99-100% with genbank p. chesapeaki sequences from france and 97% with p. chesapeaki sequences of north american origin when blast analysis was carried out. furthermore, phylogenetic s ...201424525498
understanding spatial distributions: negative density-dependence in prey causes predators to trade-off prey quantity with quality.negative density-dependence is generally studied within a single trophic level, thereby neglecting its effect on higher trophic levels. the 'functional response' couples a predator's intake rate to prey density. most widespread is a type ii functional response, where intake rate increases asymptotically with prey density; this predicts the highest predator densities at the highest prey densities. in one of the most stringent tests of this generality to date, we measured density and quality of bi ...201627053747
natural selection by pulsed predation: survival of the thickest.selective predation can lead to natural selection in prey populations and may alleviate competition among surviving individuals. the processes of selection and competition can have substantial effects on prey population dynamics, but are rarely studied simultaneously. moreover, field studies of predator-induced short-term selection pressures on prey populations are scarce. here we report measurements of density dependence in body composition in a bivalve prey (edible cockle, cerastoderma edule) ...201526378316
ingested water equilibrates isotopically with the body water pool of a shorebird with unrivaled water fluxes.we investigated the applicability of (2)h to measure the amount of body water (tbw) and water fluxes in relation to diet type and level of food intake in a mollusk-eating shorebird, the red knot (calidris canutus). six birds were exposed to eight experimental indoor conditions. average fractional (2)h turnover rates ranged between 0. 182 day(-1) (sd = 0.0219) for fasting birds and 7.759 day(-1) (sd = 0.4535) for birds feeding on cockles (cerastoderma edule). average tbw estimates obtained with t ...200011049863
ambient fauna impairs parasite transmission in a marine parasite-host system.to understand possible factors controlling transmission of trematode larvae between first and second intermediate hosts we examined the impact of ambient fauna on parasite transmission in a marine intertidal parasite-host association. cockle hosts (cerastoderma edule) kept together with selected co-occurring macrozoobenthic species in mesocosms acquired a lower parasite load compared to cockles kept alone, when targeted by cercariae of the trematode himasthla elongata. the reduction of parasite ...200818561867
amphidoma languida (amphidomatacea, dinophyceae) with a novel azaspiracid toxin profile identified as the cause of molluscan contamination at the atlantic coast of southern spain.azaspiracids (aza) are a group of food poisoning phycotoxins that are known to accumulate in shellfish. they are produced by some species of the planktonic dinophycean taxon amphidomataceae. azaspiracids have been first discovered in ireland but are now reported in shellfish from numerous global sites thus showing a wide distribution. in shellfish samples collected in 2009 near huelva (spain), aza was also found along the andalusian atlantic coast for the first time. analysis using lc-ms/ms reve ...201728118886
simulated leakage of high pco2 water negatively impacts bivalve dominated infaunal communities from the western baltic sea.carbon capture and storage is promoted as a mitigation method counteracting the increase of atmospheric co2 levels. however, at this stage, environmental consequences of potential co2 leakage from sub-seabed storage sites are still largely unknown. in a 3-month-long mesocosm experiment, this study assessed the impact of elevated pco2 levels (1,500 to 24,400 μatm) on cerastoderma edule dominated benthic communities from the baltic sea. mortality of c. edule was significantly increased in the high ...201627538361
digestive selection underlies differential utilization of phytoplankton and sedimentary organics by infaunal bivalves: experiments with cockles (cerastoderma edule) using cross-labelled mixed diets.differential utilization of phytoplankton and detrital particles present in natural sediments of mud-flats was studied in a series of experiments performed on the infaunal bivalve cerastoderma edule. in order to assess digestive selection, parameters of food processing (organic ingestion rate: oir, gross absorption efficiency: gae and gut passage time: gpt) were recorded for each organic component in different combinations of food particles radio-labelled with (14)c. experimental design included ...201627494189
widespread transmission of independent cancer lineages within multiple bivalve species.most cancers arise from oncogenic changes in the genomes of somatic cells, and while the cells may migrate by metastasis, they remain within that single individual. natural transmission of cancer cells from one individual to another has been observed in two distinct cases in mammals (tasmanian devils and dogs), but these are generally considered to be rare exceptions in nature. the discovery of transmissible cancer in soft-shell clams (mya arenaria) suggested that this phenomenon might be more w ...201627338791
impact of high pco2 on shell structure of the bivalve cerastoderma edule.raised atmospheric emissions of carbon dioxide (co2) result in an increased ocean pco2 level and decreased carbonate saturation state. ocean acidification potentially represents a major threat to calcifying organisms, specifically mollusks. the present study focuses on the impact of elevated pco2 on shell microstructural and mechanical properties of the bivalve cerastoderma edule. the mollusks were collected from the baltic sea and kept in flow-through systems at six different pco2 levels from 9 ...201627285613
influence of intertidal recreational fisheries and 'bouchot' mussel culture on bivalve recruitment.in coastal environments, fishing and aquaculture may be important sources of disturbance to ecosystem functioning, the quantification of which must be assessed to make them more sustainable. in the chausey archipelago, france, recreational fishing and commercial shellfish farming are the only two evident anthropogenic activities, dominated by bivalve hand-raking and 'bouchot' mussel culture, respectively. this study evaluates the impact of both activities on bivalve recruitment dynamics by compa ...201627039134
propensity to metal accumulation and oxidative stress responses of two benthic species (cerastoderma edule and nephtys hombergii): are tolerance processes limiting their responsiveness?the chronic exposure of benthic organisms to metals in sediments can lead to the development of tolerance mechanisms, thus diminishing their responsiveness. this study aims to evaluate the accumulation profiles of v, cr, co, ni, as, cd, pb and hg and antioxidant system responses of two benthic organisms (cerastoderma edule, bivalvia; nephtys hombergii, polychaeta). this approach will provide clarifications about the ability of each species to signalise metal contamination. organisms of both spec ...201626911481
changes of paralytic shellfish toxins in gills and digestive glands of the cockle cerastoderma edule under post-bloom natural conditions.concentrations of the paralytic shellfish toxins c1+2, c3+4, gtx5, gtx6, dcgtx2+3, dcstx, dcneo, gtx2+3, gtx1+4, stx and neo were determined by lc-fld in composite samples of digestive glands and gills of cerastoderma edule cockle. the specimens were sampled in aveiro lagoon, portugal, under natural depuration conditions (days 0, 8, 12, 14, 19, 21 and 25) after exposure to a bloom of gymnodinium catenatum. individual paralytic shellfish toxins indicated different pathways of elimination and biot ...201626874623
long-term epidemiological study of disseminated neoplasia of cockles in galicia (nw spain): temporal patterns at individual and population levels, influence of environmental and cockle-based factors and lethality.the dynamics of disseminated neoplasia (dn) affecting cockles cerastoderma edule (l.) in galicia was addressed at individual and population levels. early stage of dn was characterized by isolated neoplastic cells occurring in branchial vessels or in the connective tissue of gills, mantle, gonad or digestive gland. as disease progressed, the neoplastic cells appeared loose in foci and became widely distributed throughout the organs. in advanced stages, the connective tissue of most organs was inf ...201626813312
prevalence and genetic diversity of human sapoviruses in shellfish from commercial production areas in galicia, spain.the prevalence of human forms of sapovirus, an emerging pathogen of human gastroenteritis, was investigated in an 18-month survey from class b mollusc-harvesting areas in two galician rias (northwest spain). the detection and quantification of sapovirus was performed by reverse transcription-real-time pcr, according to the recently developed standard method iso/ts 15216-1:2013, and genotyping by reverse transcription-nested pcr. the bivalve species studied were wild and cultured mussels (mytilus ...201526655761
accumulation of sellafield-derived radiocarbon ((14)c) in irish sea and west of scotland intertidal shells and sediments.the nuclear energy industry produces radioactive waste at various stages of the fuel cycle. in the united kingdom, spent fuel is reprocessed at the sellafield facility in cumbria on the north west coast of england. waste generated at the site comprises a wide range of radionuclides including radiocarbon ((14)c) which is disposed of in various forms including highly soluble inorganic carbon within the low level liquid radioactive effluent, via pipelines into the irish sea. this (14)c is rapidly i ...201626555367
taxonomic diversity and structure of the molluscan fauna in oualidia lagoon (moroccan atlantic coast).the spatial distribution of the molluscan fauna of oualidia lagoon (moroccan atlantic coast) was studied during winter 2013. samples were collected from 43 stations over the whole of the lagoon. twenty-eight taxa (19 species of gastropods, 7 species of bivalves, 1 species of polyplacophora, and 1 species of cephalopod) were listed, 21 of which are newly reported for oualidia lagoon. four taxa, hydrobia sp. (78.29%), followed by abra alba (13.99 ), nassarius pfeifferi (5.07%), and cerastoderma ed ...201526231976
trace element fingerprinting of cockle (cerastoderma edule) shells can reveal harvesting location in adjacent areas.determining seafood geographic origin is critical for controlling its quality and safeguarding the interest of consumers. here, we use trace element fingerprinting (tef) of bivalve shells to discriminate the geographic origin of specimens. barium (ba), manganese (mn), magnesium (mg), strontium (sr) and lead (pb) were quantified in cockle shells (cerastoderma edule) captured with two fishing methods (by hand and by hand-raking) and from five adjacent fishing locations within an estuarine system ( ...201526149418
potential use of fatty acid profiles of the adductor muscle of cockles (cerastoderma edule) for traceability of collection site.geographic traceability of seafood is key for controlling its quality and safeguarding consumers' interest. the present study assessed if the fatty acid (fa) profile of the adductor muscle (am) of fresh cockles (cerastoderma edule) can be used to discriminate the origin of specimens collected in different bivalve capture/production areas legally defined within a coastal lagoon. results suggest that this biochemical approach holds the potential to trace sampling locations with a spatial resolutio ...201526084395
mesocosm validation of the marine no effect concentration of dissolved copper derived from a species sensitivity distribution.the predicted no effect concentration (pnec) for dissolved copper based on the species sensitivity distribution (ssd) of 24 marine single species tests was validated in marine mesocosms. to achieve this, the impact of actively maintained concentrations of dissolved copper on a marine benthic and planktonic community was studied in 18 outdoor 4.6m(3) mesocosms. five treatment levels, ranging from 2.9 to 31μg dissolved cu/l, were created in triplicate and maintained for 82days. clear effects were ...201525829294
profiles of paralytic shellfish toxins in bivalves of low and elevated toxicities following exposure to gymnodinium catenatum blooms in portuguese estuarine and coastal waters.profiles of paralytic shellfish toxins (psts) were examined in 405 composite samples of mytilus spp., cerastoderma edule, donax trunculus and spisula solida collected between 2007 and 2012 from natural production areas in two estuaries (aveiro and mondego), two coastal lagoons (óbidos and formosa), and three open coastal areas (aguda, comporta and culatra). toxin concentrations were obtained from the biotoxin monitoring programme database. episodes of pst toxicity in portugal have been associate ...201525616737
estimation of density-dependent mortality of juvenile bivalves in the wadden sea.we investigated density-dependent mortality within the early months of life of the bivalves macoma balthica (baltic tellin) and cerastoderma edule (common cockle) in the wadden sea. mortality is thought to be density-dependent in juvenile bivalves, because there is no proportional relationship between the size of the reproductive adult stocks and the numbers of recruits for both species. it is not known however, when exactly density dependence in the pre-recruitment phase occurs and how prevalen ...201425105293
disseminated neoplasia in the soft-shell clam mya arenaria: membrane lipid composition and functional parameters of circulating cells.in a previous study we compared lipid composition and functional parameters of circulating cells from cerastoderma edule affected or not by disseminated neoplasia (neoplastic cells vs hemocytes) (le grand et al. chem phys lipids 167:9-20 2013). neoplastic cells presented morpho-functional modifications concomitant to striking membrane lipid alterations: the proportion of particular plasmalogen molecular species was drastically decreased. we wanted to test whether this pattern was representative ...201424934587
cockle cerastoderma edule fishery collapse in the ría de arousa (galicia, nw spain) associated with the protistan parasite marteilia cochillia.the highest shellfishery catch in galicia (nw spain) has traditionally been cockle cerastoderma edule. the shellfish bed located in lombos do ulla (ría de arousa) used to be among those with the highest cockle production; however, cockle mortality rate increased sharply in this bed in april 2012, reaching 100% in may 2012. salinity and temperature were discounted as potential causes of the mortality. marteiliosis, which was first detected in february 2012 and reached 100% prevalence in april 201 ...201424781796
bmaa in shellfish from two portuguese transitional water bodies suggests the marine dinoflagellate gymnodinium catenatum as a potential bmaa source.the neurotoxin β-n-methylamino-l-alanine (bmaa) and its putative role in multiple neurodegenerative diseases have been intensely studied since 2005 when the toxin was discovered to be produced by worldwide-distributed cyanobacterial species inhabiting terrestrial, marine, brackish, and freshwater ecosystems. recently, bmaa production was also associated with one eukaryotic group, namely, diatoms, raising questions about its production by other phytoplanktonic groups. to test for bmaa bioavailabi ...201424747603
partitioning of paralytic shellfish toxins in sub-cellular fractions of the digestive gland of the cockle cerastoderma edule: changes under post-bloom natural conditions.concentrations of paralytic shellfish toxins (c1+2, b1, dcgtx2+3, dcstx, gtx2+3 and stx) were determined by lc-fld in composite samples of digestive glands of the cockle cerastoderma edule and in each sub-cellular particulate fractions obtained after differential centrifugation (nuclei+debris, mitochondria, lysosomes and microsomes). the specimens were sampled during the exposure to a bloom of gymnodinium catenatum (day 0) and in the subsequent 8, 12, 14, 19, 21 and 25 days under natural depurat ...201424736027
trematode communities in cockles (cerastoderma edule) of the ria de aveiro (portugal): influence of inorganic contamination.this work aims to assess the trematode parasites infecting the edible cockle cerastoderma edule, collected in the ria de aveiro lagoon, one of the most relevant biodiversity hotspots of the western iberia, and evaluate the relationship between the observed patterns and environmental descriptors. a total of 11 of the 16 trematode species known to infect c. edule were identified, including himasthla continua and psilostomum brevicolle as new occurrences in this lagoon. parvatrema minutum was the m ...201424680719
a mixed modeling approach to predict the effect of environmental modification on species distributions.human infrastructures can modify ecosystems, thereby affecting the occurrence and spatial distribution of organisms, as well as ecosystem functionality. sustainable development requires the ability to predict responses of species to anthropogenic pressures. we investigated the large scale, long term effect of important human alterations of benthic habitats with an integrated approach combining engineering and ecological modelling. we focused our analysis on the oosterschelde basin (the netherlan ...201424586545
limitations of metallothioneins in common cockles (cerastoderma edule) and sponges (haliclona oculata) as biomarkers of metal contamination in a semi-enclosed coastal area.poole harbour is typical of many heavily anthropologically impacted semi-enclosed estuarine coastal areas under pressure from metal pollution across the world. this study examined the physiological significance of metal burdens within that sensitive area, and assessed the potential use of metallothionein (mt) concentrations in two organisms: the common cockle (cerastoderma edule) and the mermaid's glove sponge (haliclona oculata) for mapping the spatial extent of the biological response to metal ...201424378929
ingestion and absorption of particles derived from different macrophyta in the cockle cerastoderma edule: effects of food ration.we analyzed the capacity of the common cockle cerastoderma edule to utilize detrital food particles obtained from three different macrophytes: the vascular plant juncus maritimus and two green macroalgae (ulva lactuca and enteromorpha sp.). we measured feeding and digestive parameters at three concentrations of detritus (0.5, 1.0 and 3.0 mm(3) l(-1)), so that functional relationships between ingestive and digestive processes could be assessed. increasing concentrations of detritus (food) resulte ...201424178188
assessing the spatial variability, level and source of organic chemical contaminants in bivalve fishing grounds on the galician coast (nw spain).polychlorinated biphenyls, organochlorine pesticides, organotin compounds and triazines were quantified in sediments and bivalves collected in four areas on the galician coast. one or several species were analysed at each site depending on their availability, including mussels (mytilus galloprovincialis), cockles (cerastoderma edule), clams (venerupis pullastra and ruditapes decussatus) and razor shells (ensis siliqua). the general spatial distribution of contaminants was consistent in spite of ...201323849952
cytomorphology and pcna expression pattern in bivalves mytilus galloprovincialis and cerastoderma edule with haemic neoplasia.haemic neoplasia (hn) is a pathologic condition reported in several bivalve species in different geographic areas. in this study we describe the cytomorphological features and the proliferative behaviour, assessed by the proliferating cell nuclear antigen (pcna), of hn in common cockle cerastoderma edule and mediterranean mussel mytilus galloprovicialis. in mussels the presence of at least 5 types of atypical haemocytes was detected, including a- and b-type cells, previously described in m. edul ...201323836773
conditional responses of benthic communities to interference from an intertidal bivalve.habitat-modifying organisms that impact other organisms and local functioning are important in determining ecosystem resilience. however, it is often unclear how the outcome of interactions performed by key species varies depending on the spatial and temporal disturbance context which makes the prediction of disturbance-driven regime shifts difficult. we investigated the strength and generality of effects of the filter feeding cockle cerastoderma edule on its ambient intertidal benthic physical ...201323824689
matrix solid-phase dispersion combined with gas chromatography-mass spectrometry for the determination of fifteen halogenated flame retardants in mollusks.this study presents the development and validation of a new analytical method for the simultaneous determination of fifteen analytes classified as halogenated flame retardants (hfrs) - nine brominated diphenyl ethers (bdes) and six novel hfrs - in different kinds of mollusks using matrix solid-phase dispersion (mspd) followed by gas chromatography coupled to negative chemical ionization-mass spectrometry (gc-nci-ms). the proposed method is the first one developed for such a broad range of hfrs i ...201323809805
cross-habitat interactions among bivalve species control community structure on intertidal flats.increasing evidence shows that spatial interactions between sedentary organisms can structure communities and promote landscape complexity in many ecosystems. here we tested the hypothesis that reef-forming mussels (mytilus edulis l.), a dominant intertidal ecosystem engineer in the wadden sea, promote abundances of the burrowing bivalve cerastoderma edule l. (cockle) in neighboring habitats at relatively long distances coastward from mussel beds. field surveys within and around three mussel bed ...201323691667
biomarkers and transcription levels of cancer-related genes in cockles cerastoderma edule from galicia (nw spain) with disseminated neoplasia.disseminated neoplasia (dn) is a pathological condition reported for several species of marine bivalves throughout the world, but its aetiology has not yet been satisfactorily explained. it has been suggested that chemical contamination could be a factor contributing to neoplasia. the aim of the present study was to compare cell and tissue biomarkers and the transcription level of cancer-related genes in cockles (cerastoderma edule) affected by dn with those of healthy cockles in relation to che ...201323665240
disseminated neoplasia causes changes in ploidy and apoptosis frequency in cockles cerastoderma edule.a proliferative disease, usually referred as disseminated neoplasia (dn), shows high prevalence in some cockle cerastoderma edule beds of galicia (nw spain). chromosome counts, examination of chromosome morphology, dna quantification by flow cytometry and estimation of apoptosis frequency by tunel assay and flow cytometry were performed in cockles with different dn severity. metaphases obtained from gills of dn-affected cockles displayed a chromosome number ranging from 41 to 145, while normal n ...201323583807
climate change impacts on potential recruitment in an ecosystem engineer.climate variability and the rapid warming of seas undoubtedly have huge ramifications for biological processes such as reproduction. as such, gametogenesis and spawning were investigated at two sites over 200 km apart on the south coast of ireland in an ecosystem engineer, the common cockle, cerastoderma edule. both sites are classed as special areas of conservation (sacs), but are of different water quality. cerastoderma edule plays a significant biological role by recycling nutrients and affec ...201323532482
statistical analysis of heavy metals in cerastoderma edule glaucum and venerupis aurea laeta from ganzirri lake, messina (italy).our study was carried out on two species of clams, venerupis aurea laeta and cerastoderma edule glaucum, from ganzirri lake considered as "environmental biomarkers" for their changes in physiology, morphology or distribution under the influence of substances in the environment. the aim of the present study was to conduct a statistical analysis on venerupis and cerastoderma to investigate the difference between the two autochthonous clams according to the presence of metals, and to link metal con ...201323412955
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