| plasmids isolated from the sugar beet phyllosphere show little or no homology to molecular probes currently available for plasmid typing. | from a representative sample of bacteria, isolated from mature sugar beet leaves (beta vulgaris) grown at three separate locations in the uk, 79 (18%) were shown to contain plasmids ranging in size from 10 kb to 200 kb. a sensitive colony blot method was developed to facilitate the screening of both gram-negative and gram-positive isolates to determine the distribution of known plasmid incompatibility groups among plasmids isolated from the natural environment using the collection of inc/rep pro ... | 1994 | 8180694 |
| phylogenetic analysis of erwinia species based on 16s rrna gene sequences. | the phylogenetic relationships of the type strains of 16 erwinia species were investigated by performing a comparative analysis of the sequences of the 16s rrna genes of these organisms. the sequence data were analyzed by the neighbor-joining method, and each branch was supported by moderate bootstrap values. the phylogenetic tree and sequence analyses confirmed that the genus erwinia is composed of species that exhibit considerable heterogeneity and form four clades that are intermixed with mem ... | 1997 | 9336906 |
| watermark disease in willow and its relation to brenneria salicis dominance in the wood. | | 2005 | 16637165 |
| identification of quorum sensing signal molecules and oligolignols associated with watermark disease in willow (salix sp.). | the bacterium brenneria salicis is the causal agent of watermark disease in willow. this work shows the importance of in situ studies and high-resolution separation of biological samples with ultrahigh performance liquid chromatography combined with ion trap mass spectrometry to unambiguously identify molecular compounds associated with this disease. approximately 40 oligolignols accumulated in wood sap of watermark diseased willow, and are indicative for degradation of the xylem cell wall, of w ... | 2008 | 18675599 |
| willow wood sap promotes the density-dependent pathogenesis of brenneria salicis. | brenneria salicis resides in symptomless willow (salix spp.) and other tree species, but only willow trees develop watermark disease. to understand the conversion of b. salicis into a pathogen, its pathogenicity and differential growth in the various tree species are studied. brenneria salicis was detected by plating and polymerase chain reaction-based techniques. cell wall degradation and quorum sensing (qs) were assayed as possible pathogenicity mechanisms in wood. differences in b. salicis gr ... | 2009 | 19220403 |
| brenneria salicis, the bacterium causing watermark disease in willow, resides as an endophyte in wood. | brenneria salicis has been studied in willow wood only in relation to watermark disease. in this pathogenic condition, the bacterium occurs at high concentrations. pathogenicity of b. salicis is still uncontrollable and the disease unpredictable because the plant-bacteria interaction is not understood. thanks to molecular techniques b. salicis can be detected at low concentrations, which are found in most non-pathogenic interactions. brenneria salicis was identified and traced with a new specifi ... | 2009 | 19220404 |
| genotyping of bacteria belonging to the former erwinia genus by pcr-rflp analysis of a reca gene fragment. | genotypic characterization, based on the analysis of restriction fragment length polymorphism of the reca gene fragment pcr product (reca pcr-rflp), was performed on members of the former erwinia genus. pcr primers deduced from published reca gene sequences of erwinia carotovora allowed the amplification of an approximately 730 bp dna fragment from each of the 19 erwinia species tested. amplified reca fragments were compared using rflp analysis with four endonucleases (alui, hinfi, tasi and tru1 ... | 2002 | 11832521 |
| pcr-based detection of the causal agent of watermark disease in willows (salix spp.) | the watermark disease, caused by brenneria salicis (formerly erwinia salicis), is of significant concern wherever tree-forming willows are grown or occur naturally. the movement of infected, asymptomatic cuttings is a major cause of pathogen dispersal. a reliable and sensitive diagnostic procedure is necessary for the safe movement of willow planting material. we derived primers from the nucleotide sequence of the 16s rrna gene of b. salicis for the development of a pcr to detect this pathogen. ... | 1998 | 9758827 |
| erwinia salicis: its metabolism and variability in vitro, and a method to demonstrate the pathogen in the host. | morphological, biochemical and serological features of eleven erwinia salicis isolates were examined. three groups were demonstrated, one of which comprises the dutch isolates. serological techniques proved to be a valuable addition to conventional plating techniques for detecting the pathogen. willow wood extract with 5% sucrose, 0.06% "lab lemco" broth and 1.5% agar appeared to be a suitable medium for the isolation of e. salicis, because of its selectivity. | 1976 | 1087859 |