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erwinia persicinus, a new species isolated from plants.five strains of a gram-negative, oxidase-negative, facultatively anaerobic, fermentative, motile, rod-shaped bacterium with the general characteristics of the family enterobacteriaceae were isolated from tomatoes (three strains), a banana, and a cucumber. all of the strains produced a water-soluble pink pigment. as determined by dna hybridization (hydroxyapatite method) these five strains were 85 to 100% related in 60 and 75 degrees c reactions, and related sequences exhibited 1% or less base se ...19902275853
"erwinia nulandii" is a subjective synonym of erwinia persicinus.the organism named "erwinia nulandii" was isolated in 1979 from bean seeds and was described in 1981, but the name was never validated. the results of biochemical tests and membrane protein profile and dna relatedness studies indicated that this name is synonymous with erwinia persicinus, a validly published name for a species previously isolated from, but not shown to be pathogenic for, tomatoes, bananas, and cucumbers. pathogenicity tests revealed that all e. persicinus strains, including an " ...19948186091
proferrorosamines and phytopathogenicity in erwinia spp.proferrorosamine a (pfr a) of the plant pathogenic bacterium erwinia rhapontici was shown to inhibit growth of wheat and cress seedlings at the > or = 10 ppm level. when the seeds were continuously exposed to 100 ppm pfr a, the germination of cress and wheat seeds was inhibited up to 90% and 80%, respectively. the inhibition could be reversed through addition of equimolar amounts of ferrous iron, which indicates that the strong iron chelating capability of pfr a is responsible for the observed e ...19979002178
phylogenetic analysis of erwinia species based on 16s rrna gene sequences.the phylogenetic relationships of the type strains of 16 erwinia species were investigated by performing a comparative analysis of the sequences of the 16s rrna genes of these organisms. the sequence data were analyzed by the neighbor-joining method, and each branch was supported by moderate bootstrap values. the phylogenetic tree and sequence analyses confirmed that the genus erwinia is composed of species that exhibit considerable heterogeneity and form four clades that are intermixed with mem ...19979336906
first report of a human isolate of erwinia persicinus.erwinia persicinus was first described in 1990 after being isolated from a variety of fruits and vegetables, including bananas, cucumbers, and tomatoes. in 1994, it was shown to be the causative agent of necrosis of bean pods. we now report the first human isolate of e. persicinus. the strain was isolated from the urine of an 88-year-old woman who presented with a urinary tract infection. by the hydroxyapatite method, dna from this strain was shown to be 94.5% related at 60 degrees c and 86% rel ...19989431957
reclassification of non-pigmented erwinia herbicola strains from trees as erwinia billingiae sp. nov.twenty-two erwinia-like strains, isolated from trees since the late fifties and belonging to a distinct phenotypic group with resemblance to pantoea agglomerans, were further characterized by conventional biochemical tests, the biolog metabolic fingerprinting system and fatty acid analysis. their phylogenetic positions were determined by comparing the 16s rrna gene sequence of a representative strain to available sequences of erwinia, pantoea, pectobacterium and brenneria species. the strains we ...199910319458
genotyping of bacteria belonging to the former erwinia genus by pcr-rflp analysis of a reca gene fragment.genotypic characterization, based on the analysis of restriction fragment length polymorphism of the reca gene fragment pcr product (reca pcr-rflp), was performed on members of the former erwinia genus. pcr primers deduced from published reca gene sequences of erwinia carotovora allowed the amplification of an approximately 730 bp dna fragment from each of the 19 erwinia species tested. amplified reca fragments were compared using rflp analysis with four endonucleases (alui, hinfi, tasi and tru1 ...200211832521
identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina.from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ...200212384342
structural studies on the exopolysaccharide from erwinia persicina.the gram-negative bacterial strain hki 0380 was isolated from biofilms located on palaeolithic rock paintings in the cave of bats in zuheros, southern spain. it was identified as the phytopathogenic erwinia persicina and attracted attention due to the production of considerable quantities of slime. the acidic exopolysaccharide produced by the e. persicina was studied after o-deacylation by sugar and methylation analyses, along with (1)h and (13)c nmr spectroscopy. the following structure of the ...200515992784
'candidatus erwinia dacicola', a coevolved symbiotic bacterium of the olive fly bactrocera oleae (gmelin).the taxonomic identity of the hereditary prokaryotic symbiont of the olive fly bactrocera oleae (diptera: tephritidae) was investigated. in order to avoid superficial microbial contaminants and loosely associated saprophytic biota, flies were surface-sterilized at the larval stage and reared under aseptic conditions until adult emergence. b. oleae flies originating from different geographical locations and collected at different times of the year were tested. bacterial isolation was undertaken f ...200516014495
presence of specific symbiotic bacteria in flies of the subfamily tephritinae (diptera tephritidae) and their phylogenetic relationships: proposal of 'candidatus stammerula tephritidis'.the presence of symbiotic bacteria in flies belonging to the subfamily tephritinae, which predominantly infest the flower heads of composite flowers (asteraceae), was investigated. twenty-five species of flies, collected mainly in northern italy, were examined. the bacteria adhered to the midgut epithelium in a space external to the peritrophic membrane and therefore not in direct contact with the gut contents. specific, unique and live, but unculturable bacteria were consistently found in the m ...200818523165
potential of bulb-associated bacteria for biocontrol of hyacinth soft rot caused by dickeya zeae.dickeya zeae is a pectinolytic bacterium responsible for soft rot disease in flower bulb crops. in this study, the possibility of controlling soft rot disease in hyacinth by using antagonistic bacteria isolated from hyacinth bulbs was explored.200919054227
bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri.to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage.201121204884
effect of plant growth-promoting rhizobacteria (pgprs) on plant growth, yield, and quality of tomato (lycopersicon esculentum mill.) under simulated seawater irrigation.to determine the effects of three pgprs on plant growth, yield, and quality of tomato under simulated seawater irrigation, a two consecutive seasons' field experiment was conducted in yancheng teachers university plot from april to june and august to october, 2011. the results showed that erwinia persicinus ra2 containing acc deaminase exhibited the best ability compared with bacillus pumilus wp8 and pseudomonas putida rbp1 which had no acc deaminase activity to enhance marketable yields of fres ...201222990485
bacteria associated with piezodorus guildinii (hemiptera: pentatomidae), with special reference to those transmitted by feeding.the redbanded stink bug, piezodorus guildinii (westwood) (hemiptera: heteroptera: pentatomidae), is a rapidly growing pest damaging southern us agriculture. pentatomid stink bugs are known to vector bacterial, fungal, and viral plant diseases. however, bacteria associated with redbanded stink bugs and their vector potential have not yet been assessed. in this study, we 1) cultured and identified bacteria transmitted by feeding of redbanded stink bug and 2) described bacteria from guts of redband ...201728025221
microbiological diversity associated with the spontaneous wet method of coffee fermentation.the evaluation of the microbiota present during coffee wet fermentation was done in two distinct regions of minas gerais, brazil: one farm in the south of minas gerais (lavras=l) and another farm in the savannah region (monte carmelo=mc). the yeast population ranged from 2.48 to 4.92 log cfu/g and from 2 to 4.81 log cfu/g, the mesophilic bacteria population ranged from 3.83 to 8.47 log cfu/g and from 5.37 to 7.36 log cfu/g, and the lab population ranged from 2.57 to 5.66 log cfu/g and from 3.40 ...201526119187
storage time and temperature effects on histamine production in tuna salad preparations.scombrotoxin fish poisoning (sfp), also known as histamine (hst) poisoning, has been associated with consumption of scombroid-type fish, including tuna and tuna fish products. preparation of commercial tuna salad contaminated with hstproducing bacteria (hpb), combined with time-temperature abuse, can present a food safety hazard. a potential source of hpb is raw ingredients, such as celery and onions. the objectives of this study were to determine whether raw ingredients can be a source of hpb a ...201526197286
novel phl-producing genotypes of finger millet rhizosphere associated pseudomonads and assessment of their functional and genetic diversity.genetic diversity of phld gene, an essential gene in the biosynthesis of 2,4-diacetylphloroglucinol, was studied by restriction fragment length polymorphism (rflp) in 20 phl-producing pseudomonads isolated from finger millet rhizosphere. rflp analysis of phld gene displayed three patterns with haeiii and taqi enzymes. phld gene sequence closely correlated with rflp results and revealed the existence of three new genotypes g, h and i. further, the phylogenetic and concatenated sequence analysis o ...201424819774
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